CN103274874B - Method for preparing microbial fertilizer special for spinach - Google Patents

Abstract

The invention discloses a method for preparing a microbial fertilizer special for spinach. The method specifically comprises the steps as follows: fermented cultivation of microorganism, processing of crop straws, addition of poultry excrement and wastes of a slaughter house, fermentation treatment, dissolution of an additive, mixing, drying, smashing, canning for obtaining a finished product and the like. The microbial fertilizer prepared by the invention enables yield of spinach, content of vitamin C and iron element to be increased and enables content of nitrate to be decreased, so that the spinach quality is improved greatly.

Description

A kind of preparation method of microbial fertilizer special for spinach

Technical field

The present invention relates to a kind of preparation method of microorganism fertilizer, particularly a kind of preparation method of microbial fertilizer special for spinach, belongs to fertilizer field.

Background technology

Spinach belongs to one of vegetables that people often eat, and has and mends iron effect, very popular.At present along with the raising of spinach cultivation technique and the increase of agrochemical product consumption, the output of spinach is also greatly improved, but also create minor face effect, as quality decline, taste are deteriorated, pollute aggravation etc., the favor of people to organic spinach is also more and more higher simultaneously.But the output of organic vegetable is not high, can not meet people's needs.Its concrete reason one is that fertilizer nutrient content is unstable, and the ratio of main nutrient elements is unreasonable, causes organic vegetable output significantly to reduce.Two is that some fertilizer contains objectionable impurities, such as, be that the fertilizer of main material production contains fodder additives residue with animal excrement, heavy metal etc. is contained with the fertilizer that sewage work's biochemical sludge is main material production, even if use these fertilizer spinach completely according to the operation of organic farm products production standard, the security of its product is also difficult to ensure.This just needs to change existing organic fertilizer production pattern, thus makes the nutrients release in fertilizer meet the requirement of spinach nutrition absorption, also rarely found to the research of spinach special microorganism fertilizer at present.

Summary of the invention

The technical problem that the present invention mainly solves is to provide a kind of preparation method of spinach special microorganism fertilizer, can reduce the pollution of mineral manure, can also improve the yield and quality.

For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:

A preparation method for microbial fertilizer special for spinach, comprises the following steps:

(1) fermentation culture of microorganism:

1. be inoculated on the inclined-plane of PDA substratum by bacillus amyloliquefaciens, bacillus thuringiensis, bacillusmusilaginosiengineering, the initial pH of substratum controls at 6-8; Culture temperature controls at 20-40 DEG C; Cultivate and make bacteria suspension after 12-20 hour, more in proportion 2% inoculum size by the bacterial suspension inoculation for preparing in PDA liquid nutrient medium, (shaking table revolution is 160 revs/min) is cultivated in concussion, and temperature controls at 25-30 DEG C, cultivates 48-72 hour;

2. frankia, fish spore bacterium, Geodermatophilus are being improved on Camp-BAP substratum, the initial pH of substratum controls about 7, temperature is 20-25 DEG C, cultivate 12-16 days, increase the temperature to 28-30 DEG C to continue to cultivate 10-14 days, the inoculum size of 1% is inoculated in improvement Camp-BAP liquid nutrient medium and ferments in proportion again, at 25-30 DEG C of temperature, cultivate 72-96 hour;

(2) process of crop material:

Spread maize straw, cotton stalk, soybean stalk out airing to moisture content below 10%; Then smashed in cutting machine internal cutting by above-mentioned stalk, the length after cutting is 1.5-2cm;

(3) feces of livestock and poultry and slaughterhouse waste is added:

Pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste that weight ratio is 2:3:1:1 is added in crop material after being pulverized; The weight ratio of adding above-mentioned substance and stalk is 50: 30-50, both is mixed after interpolation;

(4) ferment: the above-mentioned each material got ready is put into fermenter, evenly add cultured microorganism species simultaneously, and add brown sugar and water, mixture (maize straw, cotton stalk, soybean stalk, pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste): water: microorganism species: the weight ratio of brown sugar is 1000-1200:200-400:1:2; Throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 1-2 days, by mixture covered rearing with plastic film, become thoroughly decomposed time 2-3 days, when finding temperature more than 65 DEG C, open film and mixture carried out overturning, the timed interval be 1-2 days once, overturning 3-4 time, and then by the process of plastics film jack, every square metre of slotting 10-20 1-2cm ²sky, suitably strengthen amount of oxygen, temperature controls at 45-50 DEG C, when discovery material loosens, does not have the stink that material is original, and slightly ammonia taste, ferments complete, obtain the fermented product that moisture content is 30-40% when producing white hypha in heap;

(5) dissolving of additive: the ratio being 2:1:3:4 according to weight ratio by calcium superphosphate, boron mud, potassium fulvate, water mixes, stirs, is stirred to always and dissolves completely;

(6) joined by the material in step (6) in the fermented product that step (5) produces, mix, the weight ratio of fermented product and additive is 80-100:1;

(7) dry, pulverize: dried through fluidized-bed by above-mentioned material, temperature controls below 80 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 60 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 50-60 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product.

The invention has the beneficial effects as follows:

(1) raw material of special microorganism fertilizer of the present invention does not contain hazardous substance, and does not pollute, to product safety to soil;

(2) bacterial classification that the present invention adopts not only has the effect such as fermented stalk and ight soil, and these microorganism species can continue breeding in being manured into soil, and the bacterial classification in soil coexists, and has protection plant, the effect of kill pests;

(3) materials such as the stalk utilizing microorganism species fermentative processing to cross and ight soil have passed through the stage of becoming thoroughly decomposed fully, have thorough, sufficient feature of fermenting of becoming thoroughly decomposed, are more conducive to the absorption of spinach;

(4) in microbial fertilizer, add the materials such as calcium superphosphate, boron mud, potassium fulvate, effective solution spinach is to the demand of phosphorus, calcium, potassium and other nutritive substance, and make the Ye Geng great of spinach, output is higher.

Embodiment

Below preferred embodiment of the present invention is described in detail, can be easier to make advantages and features of the invention be readily appreciated by one skilled in the art, thus more explicit defining is made to protection scope of the present invention.

embodiment 1

A preparation method for microbial fertilizer special for spinach, comprises the following steps:

(1) fermentation culture of microorganism:

1. be inoculated on the inclined-plane of PDA substratum by bacillus amyloliquefaciens, bacillus thuringiensis, bacillusmusilaginosiengineering, the initial pH of substratum controls 6; Culture temperature controls at 20 DEG C; Cultivate and make bacteria suspension after 20 hours, more in proportion 2% inoculum size by the bacterial suspension inoculation for preparing in PDA liquid nutrient medium, (shaking table revolution is 160 revs/min) is cultivated in concussion, and temperature controls at 30 DEG C, cultivates 48 hours;

2. frankia, fish spore bacterium, Geodermatophilus are being improved on Camp-BAP substratum, the initial pH of substratum controls about 7, temperature is 20 DEG C, cultivate 16 days, increase the temperature to 30 DEG C and continue cultivation 10 days, the inoculum size of 1% is inoculated in improvement Camp-BAP liquid nutrient medium and ferments in proportion again, cultivates 72 hours at 25-30 DEG C of temperature;

(2) process of crop material:

Spread maize straw, cotton stalk, soybean stalk out airing to moisture content below 10%; Then smashed in cutting machine internal cutting by above-mentioned stalk, the length after cutting is 1.5cm;

(3) feces of livestock and poultry and slaughterhouse waste is added:

Pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste that weight ratio is 2:3:1:1 is added in crop material after being pulverized; The weight ratio of adding above-mentioned substance and stalk is 50: 30, both is mixed after interpolation;

(4) ferment: the above-mentioned each material got ready is put into fermenter, evenly add cultured microorganism species simultaneously, and add brown sugar and water, mixture (maize straw, cotton stalk, soybean stalk, pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste): water: microorganism species: the weight ratio of brown sugar is 1000:200:1:2; Throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 1 day, by mixture covered rearing with plastic film, become thoroughly decomposed 3 days time, when finding temperature more than 65 DEG C, open film and mixture carried out overturning, the timed interval be 1 day once, overturning 3-4 time, and then by the process of plastics film jack, insert 10 2cm for every square metre ²sky, suitably strengthen amount of oxygen, temperature controls at 45 DEG C, when discovery material loosens, does not have the stink that material is original, and slightly ammonia taste, ferments complete, obtain the fermented product that moisture content is 30% when producing white hypha in heap;

(5) dissolving of additive: the ratio being 2:1:3:4 according to weight ratio by calcium superphosphate, boron mud, potassium fulvate, water mixes, stirs, is stirred to always and dissolves completely;

(6) joined by the material in step (6) in the fermented product that step (5) produces, mix, the weight ratio of fermented product and additive is 80:1;

(7) dry, pulverize: dried through fluidized-bed by above-mentioned material, temperature controls below 80 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 60 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 50 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product.

embodiment 2

A preparation method for microbial fertilizer special for spinach, comprises the following steps:

(1) fermentation culture of microorganism:

1. be inoculated on the inclined-plane of PDA substratum by bacillus amyloliquefaciens, bacillus thuringiensis, bacillusmusilaginosiengineering, the initial pH of substratum controls 7; Culture temperature controls at 30 DEG C; Cultivate and make bacteria suspension after 16 hours, more in proportion 2% inoculum size by the bacterial suspension inoculation for preparing in PDA liquid nutrient medium, (shaking table revolution is 160 revs/min) is cultivated in concussion, and temperature controls at 28 DEG C, cultivates 56 hours;

2. frankia, fish spore bacterium, Geodermatophilus are being improved on Camp-BAP substratum, the initial pH of substratum controls about 7, temperature is 22 DEG C, cultivate 14 days, increase the temperature to 29 DEG C and continue cultivation 12 days, the inoculum size of 1% is inoculated in improvement Camp-BAP liquid nutrient medium and ferments in proportion again, cultivates 84 hours at 28 DEG C of temperature;

(2) process of crop material:

Spread maize straw, cotton stalk, soybean stalk out airing to moisture content below 10%; Then smashed in cutting machine internal cutting by above-mentioned stalk, the length after cutting is 1.5cm;

(3) feces of livestock and poultry and slaughterhouse waste is added:

Pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste that weight ratio is 2:3:1:1 is added in crop material after being pulverized; The weight ratio of adding above-mentioned substance and stalk is 50: 40, both is mixed after interpolation;

(4) ferment: the above-mentioned each material got ready is put into fermenter, evenly add cultured microorganism species simultaneously, and add brown sugar and water, mixture (maize straw, cotton stalk, soybean stalk, pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste): water: microorganism species: the weight ratio of brown sugar is 1100:300:1:2; Throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 2 days, by mixture covered rearing with plastic film, become thoroughly decomposed 2 days time, when finding temperature more than 65 DEG C, open film and mixture carried out overturning, the timed interval be 1 day once, overturning 4 times, and then by the process of plastics film jack, insert 15 1.5cm for every square metre ²sky, suitably strengthen amount of oxygen, temperature controls at 48 DEG C, when discovery material loosens, does not have the stink that material is original, and slightly ammonia taste, ferments complete, obtain the fermented product that moisture content is 35% when producing white hypha in heap;

(5) dissolving of additive: the ratio being 2:1:3:4 according to weight ratio by calcium superphosphate, boron mud, potassium fulvate, water mixes, stirs, is stirred to always and dissolves completely;

(6) joined by the material in step (6) in the fermented product that step (5) produces, mix, the weight ratio of fermented product and additive is 90:1;

(7) dry, pulverize: dried through fluidized-bed by above-mentioned material, temperature controls below 80 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 60 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 55 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product.

embodiment 3

1, a preparation method for microbial fertilizer special for spinach, is characterized in that, comprises the following steps:

(1) fermentation culture of microorganism:

1. be inoculated on the inclined-plane of PDA substratum by bacillus amyloliquefaciens, bacillus thuringiensis, bacillusmusilaginosiengineering, the initial pH of substratum controls 8; Culture temperature controls at 40 DEG C; Cultivate and make bacteria suspension after 12 hours, more in proportion 2% inoculum size by the bacterial suspension inoculation for preparing in PDA liquid nutrient medium, (shaking table revolution is 160 revs/min) is cultivated in concussion, and temperature controls at 30 DEG C, cultivates 48 hours;

2. frankia, fish spore bacterium, Geodermatophilus are being improved on Camp-BAP substratum, the initial pH of substratum controls about 7, temperature is 25 DEG C, cultivate 12 days, increase the temperature to 28 DEG C and continue cultivation 14 days, the inoculum size of 1% is inoculated in improvement Camp-BAP liquid nutrient medium and ferments in proportion again, cultivates 72 hours at 30 DEG C of temperature;

(2) process of crop material:

Spread maize straw, cotton stalk, soybean stalk out airing to moisture content below 10%; Then smashed in cutting machine internal cutting by above-mentioned stalk, the length after cutting is 2cm;

(3) feces of livestock and poultry and slaughterhouse waste is added:

Pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste that weight ratio is 2:3:1:1 is added in crop material after being pulverized; The weight ratio of adding above-mentioned substance and stalk is 50: 50, both is mixed after interpolation;

(4) ferment: the above-mentioned each material got ready is put into fermenter, evenly add cultured microorganism species simultaneously, and add brown sugar and water, mixture (maize straw, cotton stalk, soybean stalk, pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste): water: microorganism species: the weight ratio of brown sugar is 1200:400:1:2; Throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 2 days, by mixture covered rearing with plastic film, become thoroughly decomposed 3 days time, when finding temperature more than 65 DEG C, open film and mixture carried out overturning, the timed interval be 2 days once, overturning 3 times, and then by the process of plastics film jack, insert 20 1cm for every square metre ²sky, suitably strengthen amount of oxygen, temperature controls at 50 DEG C, when discovery material loosens, does not have the stink that material is original, and slightly ammonia taste, ferments complete, obtain the fermented product that moisture content is 40% when producing white hypha in heap;

(5) dissolving of additive: the ratio being 2:1:3:4 according to weight ratio by calcium superphosphate, boron mud, potassium fulvate, water mixes, stirs, is stirred to always and dissolves completely;

(6) joined by the material in step (6) in the fermented product that step (5) produces, mix, the weight ratio of fermented product and additive is 100:1;

(7) dry, pulverize: dried through fluidized-bed by above-mentioned material, temperature controls below 80 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 60 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 60 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product.

The bio-organic fertilizer (contrast) that the microbial fertilizer obtain embodiment 1, embodiment 2, embodiment 3 and Guangzhou Nongguan Biotech Co., Ltd. produce is applied to spinach respectively, and spinach is unified plantation, outdoor cropping, unified management; Embodiment is identical with the fertilizer amount of contrast.Respectively its output, vitamins C, ferro element, nitrate content and contrast are compared, obtain its comparing result.Concrete as table 1:

Microbial fertilizer prepared by table 1 the present invention and contrast fertilizer compare

	Output increases (%)	Vitamins C increment (%)	Ferro element increment (%)	Nitrate reduction (%)
					Embodiment 1	12	10	15	30
Embodiment 2	15	12	18	35
					Embodiment 3	13	9	13	26

Find through contrast, microbial fertilizer prepared by the present invention, can make the output of spinach, ascorbic content and ferro element all be increased, and the content of nitrate reduces, and the quality of spinach is greatly enhanced.

The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.

Claims (3)

1. a preparation method for microbial fertilizer special for spinach, comprises the following steps:

(1) fermentation culture of microorganism:

1. be inoculated on the inclined-plane of PDA substratum by bacillus amyloliquefaciens, bacillus thuringiensis, bacillusmusilaginosiengineering, the initial pH of substratum controls 6; Culture temperature controls at 20 DEG C; Cultivate and make bacteria suspension after 20 hours, more in proportion 2% inoculum size by the bacterial suspension inoculation for preparing in PDA liquid nutrient medium, concussion is cultivated, and shaking table revolution when described concussion is cultivated is 160 revs/min, and temperature controls at 30 DEG C, cultivates 48 hours;

2. frankia, fish spore bacterium, Geodermatophilus are being improved on Camp-BAP substratum, the initial pH of substratum controls about 7, temperature is 20 DEG C, cultivate 16 days, increase the temperature to 30 DEG C and continue cultivation 10 days, the inoculum size of 1% is inoculated in improvement Camp-BAP liquid nutrient medium and ferments in proportion again, cultivates 72 hours at 25-30 DEG C of temperature;

(2) process of crop material:

Spread maize straw, cotton stalk, soybean stalk out airing to moisture content below 10%; Then smashed in cutting machine internal cutting by above-mentioned stalk, the length after cutting is 1.5cm;

(3) feces of livestock and poultry and slaughterhouse waste is added:

Pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste that weight ratio is 2:3:1:1 is added in crop material after being pulverized; The weight ratio of adding above-mentioned substance and stalk is 50: 30, both is mixed after interpolation;

(4) ferment: the above-mentioned each material got ready is put into fermenter, evenly add cultured microorganism species simultaneously, and add brown sugar and water, mixture comprises maize straw, cotton stalk, soybean stalk, pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste: water: microorganism species: the weight ratio of brown sugar is 1000:200:1:2; Throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 1 day, by mixture covered rearing with plastic film, become thoroughly decomposed 3 days time, when finding temperature more than 65 DEG C, open film and mixture carried out overturning, the timed interval be 1 day once, overturning 3-4 time, and then by the process of plastics film jack, insert 10 2cm for every square metre ²sky, suitably strengthen amount of oxygen, temperature controls at 45 DEG C, when discovery material loosens, does not have the stink that material is original, and slightly ammonia taste, ferments complete, obtain the fermented product that moisture content is 30% when producing white hypha in heap;

(5) dissolving of additive: the ratio being 2:1:3:4 according to weight ratio by calcium superphosphate, boron mud, potassium fulvate, water mixes, stirs, is stirred to always and dissolves completely;

(6) joined by the material in step (5) in the fermented product that step (4) produces, mix, the weight ratio of fermented product and additive is 80:1;

(7) dry, pulverize: dried through fluidized-bed by above-mentioned material, temperature controls below 80 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 60 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 50 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product.

2. a preparation method for microbial fertilizer special for spinach, comprises the following steps:

(1) fermentation culture of microorganism:

1. be inoculated on the inclined-plane of PDA substratum by bacillus amyloliquefaciens, bacillus thuringiensis, bacillusmusilaginosiengineering, the initial pH of substratum controls 7; Culture temperature controls at 30 DEG C; Cultivate and make bacteria suspension after 16 hours, more in proportion 2% inoculum size by the bacterial suspension inoculation for preparing in PDA liquid nutrient medium, concussion is cultivated, and shaking table revolution when described concussion is cultivated is 160 revs/min, and temperature controls at 28 DEG C, cultivates 56 hours;

2. frankia, fish spore bacterium, Geodermatophilus are being improved on Camp-BAP substratum, the initial pH of substratum controls about 7, temperature is 22 DEG C, cultivate 14 days, increase the temperature to 29 DEG C and continue cultivation 12 days, the inoculum size of 1% is inoculated in improvement Camp-BAP liquid nutrient medium and ferments in proportion again, cultivates 84 hours at 28 DEG C of temperature;

(2) process of crop material:

Spread maize straw, cotton stalk, soybean stalk out airing to moisture content below 10%; Then smashed in cutting machine internal cutting by above-mentioned stalk, the length after cutting is 1.5cm;

(3) feces of livestock and poultry and slaughterhouse waste is added:

Pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste that weight ratio is 2:3:1:1 is added in crop material after being pulverized; The weight ratio of adding above-mentioned substance and stalk is 50: 40, both is mixed after interpolation;

(4) ferment: the above-mentioned each material got ready is put into fermenter, evenly add cultured microorganism species simultaneously, and add brown sugar and water, mixture comprises maize straw, cotton stalk, soybean stalk, pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste: water: microorganism species: the weight ratio of brown sugar is 1100:300:1:2; Throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 2 days, by mixture covered rearing with plastic film, become thoroughly decomposed 2 days time, when finding temperature more than 65 DEG C, open film and mixture carried out overturning, the timed interval be 1 day once, overturning 4 times, and then by the process of plastics film jack, insert 15 1.5cm for every square metre ²sky, suitably strengthen amount of oxygen, temperature controls at 48 DEG C, when discovery material loosens, does not have the stink that material is original, and slightly ammonia taste, ferments complete, obtain the fermented product that moisture content is 35% when producing white hypha in heap;

(5) dissolving of additive: the ratio being 2:1:3:4 according to weight ratio by calcium superphosphate, boron mud, potassium fulvate, water mixes, stirs, is stirred to always and dissolves completely;

(6) joined by the material in step (5) in the fermented product that step (4) produces, mix, the weight ratio of fermented product and additive is 90:1;

(7) dry, pulverize: dried through fluidized-bed by above-mentioned material, temperature controls below 80 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 60 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 55 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product.

3. a preparation method for microbial fertilizer special for spinach, is characterized in that, comprises the following steps:

(1) fermentation culture of microorganism:

1. be inoculated on the inclined-plane of PDA substratum by bacillus amyloliquefaciens, bacillus thuringiensis, bacillusmusilaginosiengineering, the initial pH of substratum controls 8; Culture temperature controls at 40 DEG C; Cultivate and make bacteria suspension after 12 hours, more in proportion 2% inoculum size by the bacterial suspension inoculation for preparing in PDA liquid nutrient medium, concussion is cultivated, and shaking table revolution when described concussion is cultivated is 160 revs/min, and temperature controls at 30 DEG C, cultivates 48 hours;

2. frankia, fish spore bacterium, Geodermatophilus are being improved on Camp-BAP substratum, the initial pH of substratum controls about 7, temperature is 25 DEG C, cultivate 12 days, increase the temperature to 28 DEG C and continue cultivation 14 days, the inoculum size of 1% is inoculated in improvement Camp-BAP liquid nutrient medium and ferments in proportion again, cultivates 72 hours at 30 DEG C of temperature;

(2) process of crop material:

Spread maize straw, cotton stalk, soybean stalk out airing to moisture content below 10%; Then smashed in cutting machine internal cutting by above-mentioned stalk, the length after cutting is 2cm;

(3) feces of livestock and poultry and slaughterhouse waste is added:

Pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste that weight ratio is 2:3:1:1 is added in crop material after being pulverized; The weight ratio of adding above-mentioned substance and stalk is 50: 50, both is mixed after interpolation;

(4) ferment: the above-mentioned each material got ready is put into fermenter, evenly add cultured microorganism species simultaneously, and add brown sugar and water, mixture comprises maize straw, cotton stalk, soybean stalk, pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste: water: microorganism species: the weight ratio of brown sugar is 1200:400:1:2; Throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 2 days, by mixture covered rearing with plastic film, become thoroughly decomposed 3 days time, when finding temperature more than 65 DEG C, open film and mixture carried out overturning, the timed interval be 2 days once, overturning 3 times, and then by the process of plastics film jack, insert 20 1 cm for every square metre ²sky, suitably strengthen amount of oxygen, temperature controls at 50 DEG C, when discovery material loosens, does not have the stink that material is original, and slightly ammonia taste, ferments complete, obtain the fermented product that moisture content is 40% when producing white hypha in heap;

(5) dissolving of additive: the ratio being 2:1:3:4 according to weight ratio by calcium superphosphate, boron mud, potassium fulvate, water mixes, stirs, is stirred to always and dissolves completely;

(6) joined by the material in step (5) in the fermented product that step (4) produces, mix, the weight ratio of fermented product and additive is 100:1;

(7) dry, pulverize: dried through fluidized-bed by above-mentioned material, temperature controls below 80 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 60 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 60 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product.