CN103053792B - Production process of composite probiotics - Google Patents
Production process of composite probiotics Download PDFInfo
- Publication number
- CN103053792B CN103053792B CN201310007392.XA CN201310007392A CN103053792B CN 103053792 B CN103053792 B CN 103053792B CN 201310007392 A CN201310007392 A CN 201310007392A CN 103053792 B CN103053792 B CN 103053792B
- Authority
- CN
- China
- Prior art keywords
- bacterium liquid
- bacillus subtilis
- bifidobacterium
- lactic acid
- acid bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000006041 probiotic Substances 0.000 title claims abstract description 41
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 41
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 20
- 239000002131 composite material Substances 0.000 title abstract 3
- 241000894006 Bacteria Species 0.000 claims abstract description 194
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 152
- 238000000855 fermentation Methods 0.000 claims abstract description 91
- 230000004151 fermentation Effects 0.000 claims abstract description 91
- 239000000463 material Substances 0.000 claims abstract description 89
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 88
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 88
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 88
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 76
- 239000004310 lactic acid Substances 0.000 claims abstract description 76
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 76
- 238000001035 drying Methods 0.000 claims abstract description 50
- 238000009395 breeding Methods 0.000 claims abstract description 37
- 230000001488 breeding effect Effects 0.000 claims abstract description 37
- 239000011343 solid material Substances 0.000 claims abstract description 32
- 230000001954 sterilising effect Effects 0.000 claims abstract description 31
- 239000001963 growth medium Substances 0.000 claims abstract description 22
- 238000001816 cooling Methods 0.000 claims abstract description 20
- 238000002156 mixing Methods 0.000 claims abstract description 17
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 10
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims description 141
- 238000010790 dilution Methods 0.000 claims description 79
- 239000012895 dilution Substances 0.000 claims description 79
- 150000001875 compounds Chemical class 0.000 claims description 38
- 238000011081 inoculation Methods 0.000 claims description 38
- 230000000529 probiotic effect Effects 0.000 claims description 37
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 27
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 18
- 239000012153 distilled water Substances 0.000 claims description 18
- 238000012856 packing Methods 0.000 claims description 18
- 238000007789 sealing Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 238000005516 engineering process Methods 0.000 claims description 16
- 239000002054 inoculum Substances 0.000 claims description 15
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 12
- 108010089934 carbohydrase Proteins 0.000 claims description 12
- 230000001580 bacterial effect Effects 0.000 claims description 10
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 claims description 10
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 229920001817 Agar Polymers 0.000 claims description 9
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 9
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 9
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 9
- 239000008272 agar Substances 0.000 claims description 9
- 238000009835 boiling Methods 0.000 claims description 9
- 238000004140 cleaning Methods 0.000 claims description 9
- CEYULKASIQJZGP-UHFFFAOYSA-L disodium;2-(carboxymethyl)-2-hydroxybutanedioate Chemical compound [Na+].[Na+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O CEYULKASIQJZGP-UHFFFAOYSA-L 0.000 claims description 9
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 9
- 239000011790 ferrous sulphate Substances 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 9
- 238000009413 insulation Methods 0.000 claims description 9
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 9
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 9
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 9
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 9
- 235000002867 manganese chloride Nutrition 0.000 claims description 9
- 239000011565 manganese chloride Substances 0.000 claims description 9
- 229940099607 manganese chloride Drugs 0.000 claims description 9
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 9
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 9
- 238000010298 pulverizing process Methods 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- SEEPANYCNGTZFQ-UHFFFAOYSA-N sulfadiazine Chemical compound C1=CC(N)=CC=C1S(=O)(=O)NC1=NC=CC=N1 SEEPANYCNGTZFQ-UHFFFAOYSA-N 0.000 claims description 9
- 229960004306 sulfadiazine Drugs 0.000 claims description 9
- 238000010792 warming Methods 0.000 claims description 9
- 235000015099 wheat brans Nutrition 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 238000000034 method Methods 0.000 abstract description 4
- 238000009776 industrial production Methods 0.000 abstract description 3
- 238000004806 packaging method and process Methods 0.000 abstract 2
- 238000010924 continuous production Methods 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000007865 diluting Methods 0.000 abstract 1
- 239000003085 diluting agent Substances 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 238000011031 large-scale manufacturing process Methods 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 description 7
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 239000003910 polypeptide antibiotic agent Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 208000031295 Animal disease Diseases 0.000 description 1
- 108010001478 Bacitracin Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 244000057717 Streptococcus lactis Species 0.000 description 1
- 235000014897 Streptococcus lactis Nutrition 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229960003071 bacitracin Drugs 0.000 description 1
- 229930184125 bacitracin Natural products 0.000 description 1
- CLKOFPXJLQSYAH-ABRJDSQDSA-N bacitracin A Chemical compound C1SC([C@@H](N)[C@@H](C)CC)=N[C@@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]1C(=O)N[C@H](CCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2N=CNC=2)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)NCCCC1 CLKOFPXJLQSYAH-ABRJDSQDSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 244000005709 gut microbiome Species 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a production process of composite probiotics. The process comprises the following specific steps of: 1, breeding, namely (1) preparing a culture solution, and heating, dissolving and saccharifying the culture solution to obtain a saccharified solution; (2) sterilizing the saccharified solution at a high temperature, and cooling the sterilized saccharified solution to obtain a breeding culture medium; and (3) inoculating strains of lactic acid bacteria, bifidobacterium, bacillus subtilis and candida utilis into the breeding culture medium for culturing, thus obtaining four mature bacterium solutions; 2, fermentation, namely (1) mixing and sterilizing bean pulp and bran, thus obtaining a solid material; (2) respectively adding the four mature bacterium solutions into a diluent, thus obtaining four diluted bacterium solutions after diluting; and (3) respectively inoculating strains of the four diluted bacterium solutions into the solid material for fermentation, thus obtaining four fermented materials; and 3, drying and packaging, namely (1) mixing the four fermented materials and drying the mixed materials for the first time; (2) drying the mixed materials for the second time; (3) drying the mixed materials for the third time; and (4) crushing, outputting and packaging the dried materials to obtain the composite probiotics. The process can realize large-scale and continuous production and is suitable for industrial production.
Description
Technical field
The invention belongs to biological technical field, relate in particular to a kind of production technology of compound probiotic.
Background technology
Fast development along with current feed industry, all feeds additive also presents diversified trend, because antibiotic various disadvantages manifests gradually, compound probiotic with its adjustable animal intestinal microflora, the good characteristic that improves digestive enzyme activity in animal body, improve breeding performonce fo animals, improve animal body immunocompetence becomes gradually the indispensable feed of livestock breeding industry and adds component.
At present, the development of China's compound probiotic and application are still in the junior stage, the production method of most compound probiotics is only laboratory preparation method's simple extension, be unsuitable for industrial production extensive, serialization, and strict not to the control of temperature, fermentation time, cause existing the problem that the shelf-life is short, probio population structure develops, biologically active is low.
Summary of the invention
The object of the invention is, for above-mentioned problems of the prior art, provides a kind of production technology of compound probiotic.
The technical scheme that the present invention adopts is for achieving the above object: a kind of production technology of compound probiotic, comprises the following steps:
(1) breeding:
1. the nutrient solution preparing is added in material-compound tank, be heated to after boiling, regulate pH value to proceed in saccharifying tank to 4.5-5.0, in saccharifying tank, add carbohydrase, be incubated saccharification 2-4h at 60 ℃, obtain saccharified liquid, wherein, the addition of carbohydrase is 300-400U/g fructus hordei germinatus;
2. saccharified liquid is proceeded in fluid heater, in 5-8s, be warming up to 95-100 ℃, insulation 1-1.5h, high-temperature sterilization, the cooling pipeline of flowing through of the saccharified liquid after sterilizing, is cooled to 30-40 ℃ in 5-8s, obtain breeding culture medium;
3. by breeding culture medium mean allocation to four chemostat, by the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis, the bacterial classification of candida utili accesses respectively in four chemostats with arrow formula inoculation device, at 30-40 ℃, constant temperature stir culture is 3 days, obtain respectively lactic acid bacteria, Bifidobacterium, bacillus subtilis, the ripe bacterium liquid of candida utili, wherein, the inoculum concentration of lactic acid bacteria is 2-4%, Bifidobacterium, the inoculum concentration of bacillus subtilis is 4-6%, the inoculum concentration of candida utili is 5-7%, lactic acid bacteria, Bifidobacterium sealing is cultivated, inoculation bacillus subtilis, the throughput of the chemostat of candida utili is 1.8L/ minute,
(2) fermentation:
1. after the dregs of beans after cleaning, pulverizing, wheat bran being mixed by the weight ratio of 5:1, pack in solid material sterilizer, at 120 ℃, keep 0.5-1h, then by the stereoscopic fermentation tank of the solid material mean allocation to four after sterilizing;
2. the ripe bacterium liquid of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili is proceeded to respectively in four thinning tanks that all fill equivalent dilution, obtain the dilution bacterium liquid of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili, wherein, the weight ratio of the gross weight of dilution gross weight and four kinds of ripe bacterium liquid is 1:1-2, the component of dilution and content are: ferrous sulfate 0.025-0.03%, sulphadiazine 0.002-0.007%, manganese chloride 0.01-0.02%, citric acid 2%, Sodium Polyacrylate 1-5%, surplus is distilled water;
3. the dilution bacterium liquid of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili is inoculated into respectively in four stereoscopic fermentation tanks that solid material is housed, 29-35 ℃ of bottom fermentation 36-48h, inoculation has the stereoscopic fermentation tank sealing and fermenting of lactic acid bacteria, Bifidobacterium, it is 3L/ minute that inoculation has the throughput of the stereoscopic fermentation tank of bacillus subtilis, candida utili, obtains the fermentation materials of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili;
(3) dry, packing:
1. first the fermentation materials of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili is added in mixer and mixed, after mixing, be transported in the first drying machine, hot blast temperature 110-120 ℃, dry 3-5s;
2. the material of the first drying machine output enters the second drying machine, and hot blast temperature is 80-100 ℃, dry 3-4s;
3. the material of the second drying machine output enters the 3rd drying machine, and hot blast temperature is 60-80 ℃, dry 2-4s;
4. by crushing material to 60 order after being dried, through the output of cooling conveying worm, packing, obtains compound probiotic.
The weight proportion of the nutrient solution of breeding phase step described in is 1.: fructus hordei germinatus 7.0-12.0g/L, peptone 6.0-8.0g/L, agar 10.0-12.0 g/L, natrium citricum 3.0-4.0 g/L, potassium dihydrogen phosphate 1.5-1.8 g/L, magnesium sulfate 0.075-0.095 g/L, sodium acid carbonate 2.0-3.0 g/L, distilled water is supplied.
Fermentation stage step 3. described in the weight ratio of lactic acid bacteria, Bifidobacterium, bacillus subtilis, the dilution bacterium liquid gross weight of candida utili and the gross weight of solid material be 1:1.2-2.
The production technology of a kind of compound probiotic of the present invention, concrete processing step is as follows:
(1) breeding:
1. 2.85kg fructus hordei germinatus, 2.1kg peptone, 3.3kg agar, 1.05kg natrium citricum, 0.495kg potassium dihydrogen phosphate, 0.0255kg magnesium sulfate, 0.75kg sodium acid carbonate are added in material-compound tank, distilled water complements to 300L, be heated to after boiling, after regulating pH value to 4.8, proceed in saccharifying tank, in saccharifying tank, add 9.98 * 10
5the carbohydrase of U, is incubated saccharification 3h at 60 ℃, obtains 311.9kg saccharified liquid;
2. 311.9kg saccharified liquid is proceeded in fluid heater, in 6s, be warming up to 98 ℃, insulation 1.3h, high-temperature sterilization, the cooling pipeline of flowing through of the saccharified liquid after sterilizing, is cooled to 35 ℃ in 7s, obtain 310.08kg breeding culture medium;
3. by 310.08kg breeding culture medium mean allocation to four chemostat, by the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis, the bacterial classification of candida utili presses 3% respectively with arrow formula inoculation device, 5%, 5%, in four chemostats of inoculum concentration access of 6%, at 35 ℃, constant temperature stir culture is 3 days, lactic acid bacteria, Bifidobacterium sealing is cultivated, inoculation bacillus subtilis, the throughput of the chemostat of candida utili is 1.8L/ minute, obtain respectively the ripe bacterium liquid of 74.52kg lactic acid bacteria, the ripe bacterium liquid of 76.84kg Bifidobacterium, the ripe bacterium liquid of 77.5kg bacillus subtilis, the ripe bacterium liquid of 77.43kg candida utili,
(2) fermentation:
1. 1054.4kg dregs of beans, 210.88kg wheat bran after cleaning, pulverizing are packed in solid material sterilizer after mixing, at 120 ℃, keep 0.8h, then by the stereoscopic fermentation tank of the solid material mean allocation to four after sterilizing;
2. by the ripe bacterium liquid of 74.52kg lactic acid bacteria, the ripe bacterium liquid of 76.84kg Bifidobacterium, the ripe bacterium liquid of 77.5kg bacillus subtilis, the ripe bacterium liquid of 77.43kg candida utili proceeds to respectively in four thinning tanks that all fill 143.93kg dilution, obtain the dilution bacterium liquid of 221.42kg lactic acid bacteria, the dilution bacterium liquid of 216.45kg Bifidobacterium, the dilution bacterium liquid of 219.67kg bacillus subtilis, the dilution bacterium liquid of 221.43kg candida utili, the component of dilution and content are: ferrous sulfate 0.028%, sulphadiazine 0.0045%, manganese chloride 0.015%, citric acid 2%, Sodium Polyacrylate 3%, surplus is distilled water,
3. by the dilution bacterium liquid of 221.42kg lactic acid bacteria, the dilution bacterium liquid of 216.45kg Bifidobacterium, the dilution bacterium liquid of 219.67kg bacillus subtilis, the dilution bacterium liquid of 221.43kg candida utili is inoculated into respectively in four stereoscopic fermentation tanks that 352.8kg solid material is housed, 32 ℃ of bottom fermentation 42h, inoculation has lactic acid bacteria, the stereoscopic fermentation tank sealing and fermenting of Bifidobacterium, inoculation has bacillus subtilis, the throughput of the stereoscopic fermentation tank of candida utili is 3L/ minute, obtain 570.25kg lactobacillus-fermented material, 571.54kg bifidus bacillus fermented product material, 572.14kg fermentation of bacillus subtilis material, 571.01kg candida utili fermentation materials,
(3) dry, packing:
1. first 570.25kg lactobacillus-fermented material, 571.54kg bifidus bacillus fermented product material, 572.14kg fermentation of bacillus subtilis material, 571.01kg candida utili fermentation materials are added in mixer and mixed, after mixing, be transported in the first drying machine, 115 ℃ of hot blast temperatures, dry 5s;
2. the material of the first drying machine output enters the second drying machine, and hot blast temperature is 90 ℃, dry 3s;
3. the material of the second drying machine output enters the 3rd drying machine, and hot blast temperature is 70 ℃, dry 3s;
4. by crushing material to 60 order after being dried, through the output of cooling conveying worm, packing, obtains 2185.12kg compound probiotic.
The production technology of a kind of compound probiotic of the present invention realizes production serialization, is convenient to control the technological parameters such as each stage material input, temperature, time, can effectively guarantee the impact on product number of viable and biological effectiveness such as temperature, time.Breeding phase adopts the TRANSIENT HIGH TEMPERATURE sterilizing of flowing, and effectively sterilization, prevents the pollution of breeding culture medium; The nutriment adding in fermentation stage dilution can promote the further increment of bacterial classification, and Sodium Polyacrylate plays good freezing action, to keep product forms; Later stage adopts staged cooling and drying, guarantees not destroy the internal structure of compound probiotic when effectively removing product moisture content, is specially adapted to large-scale industrial production.
In addition, the production technology of a kind of compound probiotic of the present invention can be secreted the multiple natural antibacterial peptides such as bacitracin, streptococcus lactis peptide when probio carries out fermented and cultured, has good antibacterial, bactericidal activity.Multiple natural antibacterial peptide coordinates compound probiotic effect can reach certain synergistic effect.
Total amount of probiotics with the compound probiotic product of explained hereafter of the present invention reaches 45,000,000,000/g, and number of viable is greater than 4 * 10
9cfu/g, antibacterial peptide content reaches 80wu/g, there is good antibacterial effect, both can be used as the effective substitute antibiotics of feed addictive and prevented Animal diseases, Escherichia coli, salmonella, staphylococcus aureus are all had to stronger inhibitory action, also can directly as medicine, effectively treat animal diarrhoea simultaneously.
The compound probiotic product of producing with the production technology of a kind of compound probiotic of the present invention, pig farm, Taizhou weanling pig of take is experimental subjects, employing take compound probiotic and antibiotic mixed in equal amounts as control group, be all the test method that compound probiotic is experimental group, carry out animal feeding test, grice diarrhoea rate: experimental group is 5.2%, control group is 17%; The piglet death rate: test group is 0, control group is 2.44%.Result of the test shows, the compound probiotic of producing with production technology of the present invention has stronger antibacterial activity.
The specific embodiment
The production technology of a kind of compound probiotic of the present invention, comprises the following steps:
(1) breeding:
1. the nutrient solution preparing is added in material-compound tank, be heated to after boiling, regulate pH value to proceed in saccharifying tank to 4.5-5.0, in saccharifying tank, add carbohydrase, be incubated saccharification 2-4h at 60 ℃, obtain saccharified liquid, wherein, the addition of carbohydrase is 300-400U/g fructus hordei germinatus;
2. saccharified liquid is proceeded in fluid heater, in 5-8s, be warming up to 95-100 ℃, insulation 1-1.5h, high-temperature sterilization, the cooling pipeline of flowing through of the saccharified liquid after sterilizing, is cooled to 30-40 ℃ in 5-8s, obtain breeding culture medium;
3. by breeding culture medium mean allocation to four chemostat, by the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis, the bacterial classification of candida utili accesses respectively in four chemostats with arrow formula inoculation device, at 30-40 ℃, constant temperature stir culture is 3 days, obtain respectively lactic acid bacteria, Bifidobacterium, bacillus subtilis, the ripe bacterium liquid of candida utili, wherein, the inoculum concentration of lactic acid bacteria is 2-4%, Bifidobacterium, the inoculum concentration of bacillus subtilis is 4-6%, the inoculum concentration of candida utili is 5-7%, lactic acid bacteria, Bifidobacterium sealing is cultivated, inoculation bacillus subtilis, the throughput of the chemostat of candida utili is 1.8L/ minute,
(2) fermentation:
1. after the dregs of beans after cleaning, pulverizing, wheat bran being mixed by the weight ratio of 5:1, pack in solid material sterilizer, at 120 ℃, keep 0.5-1h, then by the stereoscopic fermentation tank of the solid material mean allocation to four after sterilizing;
2. the ripe bacterium liquid of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili is proceeded to respectively in four thinning tanks that all fill equivalent dilution, obtain the dilution bacterium liquid of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili, wherein, the weight ratio of the gross weight of dilution gross weight and four kinds of ripe bacterium liquid is 1:1-2, the component of dilution and content are: ferrous sulfate 0.025-0.03%, sulphadiazine 0.002-0.007%, manganese chloride 0.01-0.02%, citric acid 2%, Sodium Polyacrylate 1-5%, surplus is distilled water;
3. the dilution bacterium liquid of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili is inoculated into respectively in four stereoscopic fermentation tanks that solid material is housed, 29-35 ℃ of bottom fermentation 36-48h, inoculation has the stereoscopic fermentation tank sealing and fermenting of lactic acid bacteria, Bifidobacterium, it is 3L/ minute that inoculation has the throughput of the stereoscopic fermentation tank of bacillus subtilis, candida utili, obtains the fermentation materials of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili;
(3) dry, packing:
1. first the fermentation materials of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili is added in mixer and mixed, after mixing, be transported in the first drying machine, hot blast temperature 110-120 ℃, dry 3-5s;
2. the material of the first drying machine output enters the second drying machine, and hot blast temperature is 80-100 ℃, dry 3-4s;
3. the material of the second drying machine output enters the 3rd drying machine, and hot blast temperature is 60-80 ℃, dry 2-4s;
4. by crushing material to 60 order after being dried, through the output of cooling conveying worm, packing, obtains compound probiotic.
The weight proportion of the nutrient solution of breeding phase step described in is 1.: fructus hordei germinatus 7.0-12.0g/L, peptone 6.0-8.0g/L, agar 10.0-12.0 g/L, natrium citricum 3.0-4.0 g/L, potassium dihydrogen phosphate 1.5-1.8 g/L, magnesium sulfate 0.075-0.095 g/L, sodium acid carbonate 2.0-3.0 g/L, distilled water is supplied.The culture medium that the formula of preferred nutrient solution finally makes can provide good growing environment for four kinds of probios in technique of the present invention, makes to expand increment and maximizes.
Fermentation stage step 3. described in the weight ratio of lactic acid bacteria, Bifidobacterium, bacillus subtilis, the dilution bacterium liquid gross weight of candida utili and the gross weight of solid material be 1:1.2-2.Preferred weight ratio can guarantee that the protein content in the compound probiotic product of technique of the present invention remains on suitable scope, after the animal that is conducive to accept to feed absorbs probio, antibacterial peptide, reaches optimal effectiveness.
The production technology of a kind of compound probiotic of the present invention can be also that Control System of Microcomputer is controlled by each stage material metage, input and temperature, the many places operation setting such as control of time, realizes automated production, thus further simplification of flowsheet.
The production technology of a kind of compound probiotic of the present invention can have following specific embodiment: wherein, the arrow formula inoculation device that the embodiment of the present invention adopts is 200 type arrow formula inoculation devices.
Embodiment 1, and concrete processing step is as follows:
(1) breeding:
1. 700g fructus hordei germinatus, 600g peptone, 1000g agar, 300g natrium citricum, 150g potassium dihydrogen phosphate, 7.5g magnesium sulfate, 200g sodium acid carbonate are added in material-compound tank, distilled water complements to 100L, be heated to after boiling, after regulating pH value to 4.5, proceed in saccharifying tank, in saccharifying tank, add 2.1 * 10
5the carbohydrase of U, is incubated saccharification 2h at 60 ℃, obtains 104.6kg saccharified liquid;
2. 104.6kg saccharified liquid is proceeded in fluid heater, in 5s, be warming up to 95 ℃, insulation 1h, high-temperature sterilization, the cooling pipeline of flowing through of the saccharified liquid after sterilizing, is cooled to 30 ℃ in 5s, obtain 103.8kg breeding culture medium;
3. by 103.9kg breeding culture medium mean allocation to four chemostat, by the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis, the bacterial classification of candida utili presses 2% respectively with arrow formula inoculation device, 4%, 4%, in four chemostats of inoculum concentration access of 5%, at 30 ℃, constant temperature stir culture is 3 days, lactic acid bacteria, Bifidobacterium sealing is cultivated, inoculation bacillus subtilis, the throughput of the chemostat of candida utili is 1.8L/ minute, obtain respectively the ripe bacterium liquid of 26.1kg lactic acid bacteria, the ripe bacterium liquid of 26.23kg Bifidobacterium, the ripe bacterium liquid of 26.31kg bacillus subtilis, the ripe bacterium liquid of 26.27kg candida utili,
(2) fermentation:
1. 209.71kg dregs of beans, 41.94kg wheat bran after cleaning, pulverizing are packed in solid material sterilizer after mixing, at 120 ℃, keep 0.5h, then by the stereoscopic fermentation tank of the solid material mean allocation to four after sterilizing;
2. by the ripe bacterium liquid of 26.1kg lactic acid bacteria, the ripe bacterium liquid of 26.23kg Bifidobacterium, the ripe bacterium liquid of 26.31kg bacillus subtilis, the ripe bacterium liquid of 26.27kg candida utili proceeds to respectively in four thinning tanks that all fill 26.2kg dilution, obtain the dilution bacterium liquid of 52.3kg lactic acid bacteria, the dilution bacterium liquid of 52.43kg Bifidobacterium, the dilution bacterium liquid of 52.51kg bacillus subtilis, the dilution bacterium liquid of 52.47kg candida utili, the component of dilution and content are: ferrous sulfate 0.025%, sulphadiazine 0.002%, manganese chloride 0.01%, citric acid 2%, Sodium Polyacrylate 1%, surplus is distilled water,
3. by the dilution bacterium liquid of 52.3kg lactic acid bacteria, the dilution bacterium liquid of 52.43kg Bifidobacterium, the dilution bacterium liquid of 52.51kg bacillus subtilis, the dilution bacterium liquid of 52.47kg candida utili is inoculated into respectively in four stereoscopic fermentation tanks that 62.91kg solid material is housed, 29 ℃ of bottom fermentation 36h, inoculation has lactic acid bacteria, the stereoscopic fermentation tank sealing and fermenting of Bifidobacterium, inoculation has bacillus subtilis, the throughput of the stereoscopic fermentation tank of candida utili is 3L/ minute, obtain 115.21kg lactobacillus-fermented material, 115.34kg bifidus bacillus fermented product material, 115.42kg fermentation of bacillus subtilis material, 115.38kg candida utili fermentation materials,
(3) dry, packing:
1. first 115.21kg lactobacillus-fermented material, 115.34kg bifidus bacillus fermented product material, 115.42kg fermentation of bacillus subtilis material, 115.38kg candida utili fermentation materials are added in mixer and mixed, after mixing, be transported in the first drying machine, 110 ℃ of hot blast temperatures, dry 3s;
2. the material of the first drying machine output enters the second drying machine, and hot blast temperature is 80 ℃, dry 3s;
3. the material of the second drying machine output enters the 3rd drying machine, and hot blast temperature is 60 ℃, dry 2s;
4. by crushing material to 60 order after being dried, through the output of cooling conveying worm, packing, obtains 452.42kg compound probiotic, detects compound probiotic quantity and reaches 42,500,000,000/g.
Embodiment 2, and concrete processing step is as follows:
(1) breeding:
1. 2.4kg fructus hordei germinatus, 1.6kg peptone, 2.4kg agar, 0.8kg natrium citricum, 0.36kg potassium dihydrogen phosphate, 0.019kg magnesium sulfate, 0.6kg sodium acid carbonate are added in material-compound tank, distilled water complements to 200L, be heated to after boiling, after regulating pH value to 5.0, proceed in saccharifying tank, in saccharifying tank, add 9.6 * 10
5the carbohydrase of U, is incubated saccharification 4h at 60 ℃, obtains 212.44kg saccharified liquid;
2. 212.44kg saccharified liquid is proceeded in fluid heater, in 8s, be warming up to 100 ℃, insulation 1.5h, high-temperature sterilization, the cooling pipeline of flowing through of the saccharified liquid after sterilizing, is cooled to 40 ℃ in 8s, obtain 210.18kg breeding culture medium;
3. by 210.18kg breeding culture medium mean allocation to four chemostat, by the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis, the bacterial classification of candida utili presses 4% respectively with arrow formula inoculation device, 6%, 6%, in four chemostats of inoculum concentration access of 7%, at 40 ℃, constant temperature stir culture is 3 days, lactic acid bacteria, Bifidobacterium sealing is cultivated, inoculation bacillus subtilis, the throughput of the chemostat of candida utili is 1.8L/ minute, obtain respectively the ripe bacterium liquid of 52.10kg lactic acid bacteria, the ripe bacterium liquid of 53.06kg Bifidobacterium, the ripe bacterium liquid of 52.59kg bacillus subtilis, the ripe bacterium liquid of 53.13kg candida utili,
(2) fermentation:
1. 1054.4kg dregs of beans, 210.88kg wheat bran after cleaning, pulverizing are packed in solid material sterilizer after mixing, at 120 ℃, keep 1h, then by the stereoscopic fermentation tank of the solid material mean allocation to four after sterilizing;
2. by the ripe bacterium liquid of 52.10kg lactic acid bacteria, the ripe bacterium liquid of 53.06kg Bifidobacterium, the ripe bacterium liquid of 52.59kg bacillus subtilis, the ripe bacterium liquid of 53.13kg candida utili proceeds to respectively in four thinning tanks that all fill 105.44kg dilution, obtain the dilution bacterium liquid of 157.54kg lactic acid bacteria, the dilution bacterium liquid of 158.5kg Bifidobacterium, the dilution bacterium liquid of 158.03kg bacillus subtilis, the dilution bacterium liquid of 158.57kg candida utili, the component of dilution and content are: ferrous sulfate 0.03%, sulphadiazine 0.007%, manganese chloride 0.02%, citric acid 2%, Sodium Polyacrylate 5%, surplus is distilled water,
3. by the dilution bacterium liquid of 157.54kg lactic acid bacteria, the dilution bacterium liquid of 158.5kg Bifidobacterium, the dilution bacterium liquid of 158.03kg bacillus subtilis, the dilution bacterium liquid of 158.57kg candida utili is inoculated into respectively in four stereoscopic fermentation tanks that 316.32kg solid material is housed, 35 ℃ of bottom fermentation 48h, inoculation has lactic acid bacteria, the stereoscopic fermentation tank sealing and fermenting of Bifidobacterium, inoculation has bacillus subtilis, the throughput of the stereoscopic fermentation tank of candida utili is 3L/ minute, obtain 473.86kg lactobacillus-fermented material, 474.82kg bifidus bacillus fermented product material, 474.35kg fermentation of bacillus subtilis material, 474.89kg candida utili fermentation materials,
(3) dry, packing:
1. first 473.86kg lactobacillus-fermented material, 474.82kg bifidus bacillus fermented product material, 474.35kg fermentation of bacillus subtilis material, 474.89kg candida utili fermentation materials are added in mixer and mixed, after mixing, be transported in the first drying machine, 120 ℃ of hot blast temperatures, dry 5s;
2. the material of the first drying machine output enters the second drying machine, and hot blast temperature is 100 ℃, dry 4s;
3. the material of the second drying machine output enters the 3rd drying machine, and hot blast temperature is 80 ℃, dry 4s;
4. by crushing material to 60 order after being dried, through the output of cooling conveying worm, packing, obtains 1872.92kg compound probiotic, detects compound probiotic quantity and reaches 43,500,000,000/g.
Embodiment 3, and concrete processing step is as follows:
(1) breeding:
1. 2.85kg fructus hordei germinatus, 2.1kg peptone, 3.3kg agar, 1.05kg natrium citricum, 0.495kg potassium dihydrogen phosphate, 0.0255kg magnesium sulfate, 0.75kg sodium acid carbonate are added in material-compound tank, distilled water complements to 300L, be heated to after boiling, after regulating pH value to 4.8, proceed in saccharifying tank, in saccharifying tank, add 9.98 * 10
5the carbohydrase of U, is incubated saccharification 3h at 60 ℃, obtains 311.9kg saccharified liquid;
2. 311.9kg saccharified liquid is proceeded in fluid heater, in 6s, be warming up to 98 ℃, insulation 1.3h, high-temperature sterilization, the cooling pipeline of flowing through of the saccharified liquid after sterilizing, is cooled to 35 ℃ in 7s, obtain 310.08kg breeding culture medium;
3. by 310.08kg breeding culture medium mean allocation to four chemostat, by the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis, the bacterial classification of candida utili presses 3% respectively with arrow formula inoculation device, 5%, 5%, in four chemostats of inoculum concentration access of 6%, at 35 ℃, constant temperature stir culture is 3 days, lactic acid bacteria, Bifidobacterium sealing is cultivated, inoculation bacillus subtilis, the throughput of the chemostat of candida utili is 1.8L/ minute, obtain respectively the ripe bacterium liquid of 74.52kg lactic acid bacteria, the ripe bacterium liquid of 76.84kg Bifidobacterium, the ripe bacterium liquid of 77.5kg bacillus subtilis, the ripe bacterium liquid of 77.43kg candida utili,
(2) fermentation:
1. 1054.4kg dregs of beans, 210.88kg wheat bran after cleaning, pulverizing are packed in solid material sterilizer after mixing, at 120 ℃, keep 0.8h, then by the stereoscopic fermentation tank of the solid material mean allocation to four after sterilizing;
2. by the ripe bacterium liquid of 74.52kg lactic acid bacteria, the ripe bacterium liquid of 76.84kg Bifidobacterium, the ripe bacterium liquid of 77.5kg bacillus subtilis, the ripe bacterium liquid of 77.43kg candida utili proceeds to respectively in four thinning tanks that all fill 143.93kg dilution, obtain the dilution bacterium liquid of 221.42kg lactic acid bacteria, the dilution bacterium liquid of 216.45kg Bifidobacterium, the dilution bacterium liquid of 219.67kg bacillus subtilis, the dilution bacterium liquid of 221.43kg candida utili, the component of dilution and content are: ferrous sulfate 0.028%, sulphadiazine 0.0045%, manganese chloride 0.015%, citric acid 2%, Sodium Polyacrylate 3%, surplus is distilled water,
3. by the dilution bacterium liquid of 221.42kg lactic acid bacteria, the dilution bacterium liquid of 216.45kg Bifidobacterium, the dilution bacterium liquid of 219.67kg bacillus subtilis, the dilution bacterium liquid of 221.43kg candida utili is inoculated into respectively in four stereoscopic fermentation tanks that 352.8kg solid material is housed, 32 ℃ of bottom fermentation 42h, inoculation has lactic acid bacteria, the stereoscopic fermentation tank sealing and fermenting of Bifidobacterium, inoculation has bacillus subtilis, the throughput of the stereoscopic fermentation tank of candida utili is 3L/ minute, obtain 570.25kg lactobacillus-fermented material, 571.54kg bifidus bacillus fermented product material, 572.14kg fermentation of bacillus subtilis material, 571.01kg candida utili fermentation materials,
(3) dry, packing:
1. first 570.25kg lactobacillus-fermented material, 571.54kg bifidus bacillus fermented product material, 572.14kg fermentation of bacillus subtilis material, 571.01kg candida utili fermentation materials are added in mixer and mixed, after mixing, be transported in the first drying machine, 115 ℃ of hot blast temperatures, dry 5s;
2. the material of the first drying machine output enters the second drying machine, and hot blast temperature is 90 ℃, dry 3s;
3. the material of the second drying machine output enters the 3rd drying machine, and hot blast temperature is 70 ℃, dry 3s;
4. by crushing material to 60 order after being dried, through the output of cooling conveying worm, packing, obtains 2185.12kg compound probiotic, detects compound probiotic quantity and reaches 45,000,000,000/g.
Embodiment 4, and concrete processing step is as follows:
(1) breeding:
1. 2.45kg fructus hordei germinatus, 2.8kg peptone, 3.5kg agar, 1.05kg natrium citricum, 0.63kg potassium dihydrogen phosphate, 0.0263kg magnesium sulfate, 1.05kg sodium acid carbonate are added in material-compound tank, distilled water complements to 350L, be heated to after boiling, after regulating pH value to 5.0, proceed in saccharifying tank, in saccharifying tank, add 7.84 * 10
5the carbohydrase of U, is incubated saccharification 2h at 60 ℃, obtains 368.29kg saccharified liquid;
2. 368.29kg saccharified liquid is proceeded in fluid heater, in 5s, be warming up to 100 ℃, insulation 1h, high-temperature sterilization, the cooling pipeline of flowing through of the saccharified liquid after sterilizing, is cooled to 30 ℃ in 8s, obtain 365.42kg breeding culture medium;
3. by 365.42kg breeding culture medium mean allocation to four chemostat, by the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis, the bacterial classification of candida utili presses 2% respectively with arrow formula inoculation device, 6%, 4%, in four chemostats of inoculum concentration access of 6%, at 40 ℃, constant temperature stir culture is 3 days, lactic acid bacteria, Bifidobacterium sealing is cultivated, inoculation bacillus subtilis, the throughput of the chemostat of candida utili is 1.8L/ minute, obtain respectively the ripe bacterium liquid of 90.27kg lactic acid bacteria, the ripe bacterium liquid of 89.36kg Bifidobacterium, the ripe bacterium liquid of 87.15kg bacillus subtilis, the ripe bacterium liquid of 88.54kg candida utili,
(2) fermentation:
1. 1065.95kg dregs of beans, 213.19kg wheat bran after cleaning, pulverizing are packed in solid material sterilizer after mixing, at 120 ℃, keep 1h, then by the stereoscopic fermentation tank of the solid material mean allocation to four after sterilizing;
2. by the ripe bacterium liquid of 90.27kg lactic acid bacteria, the ripe bacterium liquid of 89.36kg Bifidobacterium, the ripe bacterium liquid of 87.15kg bacillus subtilis, the ripe bacterium liquid of 88.54kg candida utili proceeds to respectively in four thinning tanks that all fill 88.83kg dilution, obtain the dilution bacterium liquid of 178.23kg lactic acid bacteria, the dilution bacterium liquid of 175.84kg Bifidobacterium, the dilution bacterium liquid of 175.9kg bacillus subtilis, the dilution bacterium liquid of 176.28kg candida utili, the component of dilution and content are: ferrous sulfate 0.025%, sulphadiazine 0.0059%, manganese chloride 0.02%, citric acid 2%, Sodium Polyacrylate 4%, surplus is distilled water,
3. by the dilution bacterium liquid of 178.23kg lactic acid bacteria, the dilution bacterium liquid of 175.84kg Bifidobacterium, the dilution bacterium liquid of 175.9kg bacillus subtilis, the dilution bacterium liquid of 176.28kg candida utili is inoculated into respectively in four stereoscopic fermentation tanks that 319.78kg solid material is housed, 30 ℃ of bottom fermentation 48h, inoculation has lactic acid bacteria, the stereoscopic fermentation tank sealing and fermenting of Bifidobacterium, inoculation has bacillus subtilis, the throughput of the stereoscopic fermentation tank of candida utili is 3L/ minute, obtain 498.28kg lactobacillus-fermented material, 497.18kg bifidus bacillus fermented product material, 493.25kg fermentation of bacillus subtilis material, 495.21kg candida utili fermentation materials,
(3) dry, packing:
1. first 498.28kg lactobacillus-fermented material, 497.18kg bifidus bacillus fermented product material, 493.25kg fermentation of bacillus subtilis material, 495.21kg candida utili fermentation materials are added in mixer and mixed, after mixing, be transported in the first drying machine, 110 ℃ of hot blast temperatures, dry 5s;
2. the material of the first drying machine output enters the second drying machine, and hot blast temperature is 80 ℃, dry 4s;
3. the material of the second drying machine output enters the 3rd drying machine, and hot blast temperature is 80 ℃, dry 2s;
4. by crushing material to 60 order after being dried, through the output of cooling conveying worm, packing, obtains 1787.96kg compound probiotic, detects compound probiotic quantity and reaches 44,000,000,000/g.
Claims (3)
1. a production technology for compound probiotic, is characterized in that: comprise the following steps:
(1) breeding:
1. the nutrient solution preparing is added in material-compound tank, be heated to after boiling, regulate pH value to proceed in saccharifying tank to 4.5-5.0, in saccharifying tank, add carbohydrase, be incubated saccharification 2-4h at 60 ℃, obtain saccharified liquid, wherein, the addition of carbohydrase is 300-400U/g fructus hordei germinatus;
2. saccharified liquid is proceeded in fluid heater, in 5-8s, be warming up to 95-100 ℃, insulation 1-1.5h, high-temperature sterilization, the cooling pipeline of flowing through of the saccharified liquid after sterilizing, is cooled to 30-40 ℃ in 5-8s, obtain breeding culture medium;
3. by breeding culture medium mean allocation to four chemostat, by the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis, the bacterial classification of candida utili accesses respectively in four chemostats with arrow formula inoculation device, at 30-40 ℃, constant temperature stir culture is 3 days, obtain respectively lactic acid bacteria, Bifidobacterium, bacillus subtilis, the ripe bacterium liquid of candida utili, wherein, the inoculum concentration of lactic acid bacteria is 2-4%, Bifidobacterium, the inoculum concentration of bacillus subtilis is 4-6%, the inoculum concentration of candida utili is 5-7%, lactic acid bacteria, Bifidobacterium sealing is cultivated, inoculation bacillus subtilis, the throughput of the chemostat of candida utili is 1.8L/ minute,
(2) fermentation:
1. after the dregs of beans after cleaning, pulverizing, wheat bran being mixed by the weight ratio of 5:1, pack in solid material sterilizer, at 120 ℃, keep 0.5-1h, then by the stereoscopic fermentation tank of the solid material mean allocation to four after sterilizing;
2. the ripe bacterium liquid of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili is proceeded to respectively in four thinning tanks that all fill equivalent dilution, obtain the dilution bacterium liquid of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili, wherein, the weight ratio of the gross weight of dilution gross weight and four kinds of ripe bacterium liquid is 1:1-2, the component of dilution and content are: ferrous sulfate 0.025-0.03%, sulphadiazine 0.002-0.007%, manganese chloride 0.01-0.02%, citric acid 2%, Sodium Polyacrylate 1-5%, surplus is distilled water;
3. the dilution bacterium liquid of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili is inoculated into respectively in four stereoscopic fermentation tanks that solid material is housed, 29-35 ℃ of bottom fermentation 36-48h, inoculation has the stereoscopic fermentation tank sealing and fermenting of lactic acid bacteria, Bifidobacterium, it is 3L/ minute that inoculation has the throughput of the stereoscopic fermentation tank of bacillus subtilis, candida utili, obtains the fermentation materials of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili;
(3) dry, packing:
1. first the fermentation materials of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili is added in mixer and mixed, after mixing, be transported in the first drying machine, hot blast temperature 110-120 ℃, dry 3-5s;
2. the material of the first drying machine output enters the second drying machine, and hot blast temperature is 80-100 ℃, dry 3-4s;
3. the material of the second drying machine output enters the 3rd drying machine, and hot blast temperature is 60-80 ℃, dry 2-4s;
4. by crushing material to 60 order after being dried, through the output of cooling conveying worm, packing, obtains compound probiotic;
The weight proportion of the nutrient solution of breeding phase step described in is 1.: fructus hordei germinatus 7.0-12.0g/L, peptone 6.0-8.0g/L, agar 10.0-12.0 g/L, natrium citricum 3.0-4.0 g/L, potassium dihydrogen phosphate 1.5-1.8 g/L, magnesium sulfate 0.075-0.095 g/L, sodium acid carbonate 2.0-3.0 g/L, distilled water is supplied.
2. the production technology of a kind of compound probiotic according to claim 1, is characterized in that: fermentation stage step 3. described in the weight ratio of lactic acid bacteria, Bifidobacterium, bacillus subtilis, the dilution bacterium liquid gross weight of candida utili and the gross weight of solid material be 1:1.2-2.
3. the production technology of a kind of compound probiotic according to claim 1, is characterized in that: concrete processing step is as follows:
(1) breeding:
1. 2.85kg fructus hordei germinatus, 2.1kg peptone, 3.3kg agar, 1.05kg natrium citricum, 0.495kg potassium dihydrogen phosphate, 0.0255kg magnesium sulfate, 0.75kg sodium acid carbonate are added in material-compound tank, distilled water complements to 300L, be heated to after boiling, after regulating pH value to 4.8, proceed in saccharifying tank, in saccharifying tank, add 9.98 * 10
5the carbohydrase of U, is incubated saccharification 3h at 60 ℃, obtains 311.9kg saccharified liquid;
2. 311.9kg saccharified liquid is proceeded in fluid heater, in 6s, be warming up to 98 ℃, insulation 1.3h, high-temperature sterilization, the cooling pipeline of flowing through of the saccharified liquid after sterilizing, is cooled to 35 ℃ in 7s, obtain 310.08kg breeding culture medium;
3. by 310.08kg breeding culture medium mean allocation to four chemostat, by the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis, the bacterial classification of candida utili presses 3% respectively with arrow formula inoculation device, 5%, 5%, in four chemostats of inoculum concentration access of 6%, at 35 ℃, constant temperature stir culture is 3 days, lactic acid bacteria, Bifidobacterium sealing is cultivated, inoculation bacillus subtilis, the throughput of the chemostat of candida utili is 1.8L/ minute, obtain respectively the ripe bacterium liquid of 74.52kg lactic acid bacteria, the ripe bacterium liquid of 76.84kg Bifidobacterium, the ripe bacterium liquid of 77.5kg bacillus subtilis, the ripe bacterium liquid of 77.43kg candida utili,
(2) fermentation:
1. 1054.4kg dregs of beans, 210.88kg wheat bran after cleaning, pulverizing are packed in solid material sterilizer after mixing, at 120 ℃, keep 0.8h, then by the stereoscopic fermentation tank of the solid material mean allocation to four after sterilizing;
2. by the ripe bacterium liquid of 74.52kg lactic acid bacteria, the ripe bacterium liquid of 76.84kg Bifidobacterium, the ripe bacterium liquid of 77.5kg bacillus subtilis, the ripe bacterium liquid of 77.43kg candida utili proceeds to respectively in four thinning tanks that all fill 143.93kg dilution, obtain the dilution bacterium liquid of 221.42kg lactic acid bacteria, the dilution bacterium liquid of 216.45kg Bifidobacterium, the dilution bacterium liquid of 219.67kg bacillus subtilis, the dilution bacterium liquid of 221.43kg candida utili, the component of dilution and content are: ferrous sulfate 0.028%, sulphadiazine 0.0045%, manganese chloride 0.015%, citric acid 2%, Sodium Polyacrylate 3%, surplus is distilled water,
3. by the dilution bacterium liquid of 221.42kg lactic acid bacteria, the dilution bacterium liquid of 216.45kg Bifidobacterium, the dilution bacterium liquid of 219.67kg bacillus subtilis, the dilution bacterium liquid of 221.43kg candida utili is inoculated into respectively in four stereoscopic fermentation tanks that 352.8kg solid material is housed, 32 ℃ of bottom fermentation 42h, inoculation has lactic acid bacteria, the stereoscopic fermentation tank sealing and fermenting of Bifidobacterium, inoculation has bacillus subtilis, the throughput of the stereoscopic fermentation tank of candida utili is 3L/ minute, obtain 570.25kg lactobacillus-fermented material, 571.54kg bifidus bacillus fermented product material, 572.14kg fermentation of bacillus subtilis material, 571.01kg candida utili fermentation materials,
(3) dry, packing:
1. first 570.25kg lactobacillus-fermented material, 571.54kg bifidus bacillus fermented product material, 572.14kg fermentation of bacillus subtilis material, 571.01kg candida utili fermentation materials are added in mixer and mixed, after mixing, be transported in the first drying machine, 115 ℃ of hot blast temperatures, dry 5s;
2. the material of the first drying machine output enters the second drying machine, and hot blast temperature is 90 ℃, dry 3s;
3. the material of the second drying machine output enters the 3rd drying machine, and hot blast temperature is 70 ℃, dry 3s;
4. by crushing material to 60 order after being dried, through the output of cooling conveying worm, packing, obtains 2185.12kg compound probiotic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310007392.XA CN103053792B (en) | 2013-01-09 | 2013-01-09 | Production process of composite probiotics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310007392.XA CN103053792B (en) | 2013-01-09 | 2013-01-09 | Production process of composite probiotics |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103053792A CN103053792A (en) | 2013-04-24 |
| CN103053792B true CN103053792B (en) | 2014-02-12 |
Family
ID=48096938
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310007392.XA Active CN103053792B (en) | 2013-01-09 | 2013-01-09 | Production process of composite probiotics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103053792B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104054903A (en) * | 2014-06-06 | 2014-09-24 | 李晓叶 | Production process of fermented cottonseed meal |
| CN106666091A (en) * | 2016-12-11 | 2017-05-17 | 唐林元 | Preparation method of enteric probiotic fermentation acidifier particles |
| CN113249253A (en) * | 2021-05-13 | 2021-08-13 | 内蒙古农业大学 | Production process of composite probiotics |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1408760A2 (en) * | 2001-02-19 | 2004-04-21 | Société des Produits Nestlé S.A. | Consumable product containing probiotics |
| CN1766095A (en) * | 2005-09-16 | 2006-05-03 | 保罗生物园科技有限公司 | Probiotic composite enzyme production method |
| CN101889631A (en) * | 2010-07-07 | 2010-11-24 | 格特生物制药（天津）有限公司 | Bacillus subtilis and lactobacillus premix with hawthorn seed auxiliary material for livestock and poultry and preparation method thereof |
| CN102356912A (en) * | 2011-11-02 | 2012-02-22 | 黑龙江省龙蛙粮油进出口有限公司 | Preparation method of probiotic fermented rice milk |
| CN102835555A (en) * | 2011-06-21 | 2012-12-26 | 纽素乐必佳（天津）药业集团有限公司 | Composite probiotics premix and preparation method thereof |
-
2013
- 2013-01-09 CN CN201310007392.XA patent/CN103053792B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1408760A2 (en) * | 2001-02-19 | 2004-04-21 | Société des Produits Nestlé S.A. | Consumable product containing probiotics |
| CN1766095A (en) * | 2005-09-16 | 2006-05-03 | 保罗生物园科技有限公司 | Probiotic composite enzyme production method |
| CN101889631A (en) * | 2010-07-07 | 2010-11-24 | 格特生物制药（天津）有限公司 | Bacillus subtilis and lactobacillus premix with hawthorn seed auxiliary material for livestock and poultry and preparation method thereof |
| CN102835555A (en) * | 2011-06-21 | 2012-12-26 | 纽素乐必佳（天津）药业集团有限公司 | Composite probiotics premix and preparation method thereof |
| CN102356912A (en) * | 2011-11-02 | 2012-02-22 | 黑龙江省龙蛙粮油进出口有限公司 | Preparation method of probiotic fermented rice milk |
Non-Patent Citations (4)
| Title |
|---|
| 《含木聚糖酶复合益生菌饲料添加剂的研制及应用》;成珍等;《饲料与畜牧》;20120731(第7期);24-26页 * |
| 《活性益生菌饲料添加剂的开发》;郑德富等;《饲养饲料》;20100831(第8期);50-51页 * |
| 成珍等.《含木聚糖酶复合益生菌饲料添加剂的研制及应用》.《饲料与畜牧》.2012,(第7期),24-26页. |
| 郑德富等.《活性益生菌饲料添加剂的开发》.《饲养饲料》.2010,(第8期),50-51页. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103053792A (en) | 2013-04-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105661011B (en) | Functional biological protein feed leavening agent and fermented protein feed | |
| CN104054902A (en) | Process for producing fermented soybean meal through solid state fermentation of mixed culture | |
| CN102178091B (en) | Baking type suckling pig feed before weaning and preparation method thereof | |
| CN105685475A (en) | Method for preparing feed antibiotic substitute from subtilin producing bacillus subtilis fermented Chinese herbal medicine | |
| CN102178035B (en) | Method for fermenting and decomposing gossypol in cottonseed meal by composite strains | |
| CN101912056B (en) | Method for preparing drug residue free feed for feeding live pigs | |
| CN105661013B (en) | A kind of method that fermentation of Chinese herbal medicine prepares compound feed additive for promoting growth | |
| CN103355472A (en) | Microbial fermentation agent for cottonseed meal detoxification and preparation method and application thereof | |
| CN105746881A (en) | Method for preparing substituent of antibiotics for feed by fermenting Chinese herbal medicines through lipopeptide-producing bacillus subtilis | |
| CN105483050A (en) | Lactobacillus plantarum strain and application thereof in fermented feed | |
| CN102234623A (en) | Microbial preparation and fermentation process thereof | |
| CN103211084A (en) | Method for preparing biological enzyme-enriched camellia seed meal fermented feed | |
| CN104782892A (en) | Bagasse fermented feed and preparation method thereof | |
| CN101214006B (en) | Method for using EM original liquid fermentation apple pomace to produce protein feed | |
| CN103478405A (en) | Method used for preparing probiotic preparations by using vitamin B2 fermentation liquid waste | |
| CN104054903A (en) | Production process of fermented cottonseed meal | |
| CN105379947A (en) | Animal feed and preparation method thereof | |
| CN103289937A (en) | Method for producing bacillus laterosporus live bacteria by high density solid fermentation | |
| CN103053792B (en) | Production process of composite probiotics | |
| CN105661012A (en) | High-temperature fermentation method of Chinese herbal medicines | |
| CN102160609B (en) | Swine fermentation complete feed and production method thereof | |
| CN110301526A (en) | Complex micro organism fungicide and its method for preparing bioactive feed | |
| CN102178038B (en) | Method for preparing fermented high-lysine high-protein feed | |
| CN103555611A (en) | Lactobacillus acidophilus culture feed additive and preparation method thereof | |
| CN101717746A (en) | Method for producing spores of clostridium butyricum and application thereof in livestock and poultry rearing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent for invention or patent application | ||
| CB02 | Change of applicant information |
Address after: Livillage Li Hai town 274916 administrative villages in Shandong province Juye County No. 076 Applicant after: Li Xiaoye Address before: 250132, room 3, unit 1, building 22, 201 Wang Dam Road, Licheng District, Shandong, Ji'nan Applicant before: Li Xiaoye |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wang Ye Inventor before: Li Xiaoye |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170915 Address after: 510000 Guangdong city of Guangzhou province high tech Industrial Development Zone Ruitailu No. 2 building first layer B Patentee after: Guangzhou puwell sincessta Pharmaceutical Co. Ltd. Address before: Livillage Li Hai town 274916 administrative villages in Shandong province Juye County No. 076 Patentee before: Li Xiaoye |
|
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wang Ye Inventor after: Liu Yangyang Inventor after: Wang Congfeng Inventor before: Wang Ye |