CN103053792B - 一种复合益生菌的生产工艺 - Google Patents
一种复合益生菌的生产工艺 Download PDFInfo
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- CN103053792B CN103053792B CN201310007392.XA CN201310007392A CN103053792B CN 103053792 B CN103053792 B CN 103053792B CN 201310007392 A CN201310007392 A CN 201310007392A CN 103053792 B CN103053792 B CN 103053792B
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- bacterium liquid
- bacillus subtilis
- bifidobacterium
- lactic acid
- acid bacteria
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- 239000006041 probiotic Substances 0.000 title claims abstract description 41
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 41
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 20
- 239000002131 composite material Substances 0.000 title abstract 3
- 241000894006 Bacteria Species 0.000 claims abstract description 194
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 152
- 238000000855 fermentation Methods 0.000 claims abstract description 91
- 230000004151 fermentation Effects 0.000 claims abstract description 91
- 239000000463 material Substances 0.000 claims abstract description 89
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 88
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 88
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 88
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 76
- 239000004310 lactic acid Substances 0.000 claims abstract description 76
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 76
- 238000001035 drying Methods 0.000 claims abstract description 50
- 238000009395 breeding Methods 0.000 claims abstract description 37
- 230000001488 breeding effect Effects 0.000 claims abstract description 37
- 239000011343 solid material Substances 0.000 claims abstract description 32
- 230000001954 sterilising effect Effects 0.000 claims abstract description 31
- 239000001963 growth medium Substances 0.000 claims abstract description 22
- 238000001816 cooling Methods 0.000 claims abstract description 20
- 238000002156 mixing Methods 0.000 claims abstract description 17
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 10
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims description 141
- 238000010790 dilution Methods 0.000 claims description 79
- 239000012895 dilution Substances 0.000 claims description 79
- 150000001875 compounds Chemical class 0.000 claims description 38
- 238000011081 inoculation Methods 0.000 claims description 38
- 230000000529 probiotic effect Effects 0.000 claims description 37
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 27
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 18
- 239000012153 distilled water Substances 0.000 claims description 18
- 238000012856 packing Methods 0.000 claims description 18
- 238000007789 sealing Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 238000005516 engineering process Methods 0.000 claims description 16
- 239000002054 inoculum Substances 0.000 claims description 15
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 12
- 108010089934 carbohydrase Proteins 0.000 claims description 12
- 230000001580 bacterial effect Effects 0.000 claims description 10
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 claims description 10
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 229920001817 Agar Polymers 0.000 claims description 9
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 9
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 9
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 9
- 239000008272 agar Substances 0.000 claims description 9
- 238000009835 boiling Methods 0.000 claims description 9
- 238000004140 cleaning Methods 0.000 claims description 9
- CEYULKASIQJZGP-UHFFFAOYSA-L disodium;2-(carboxymethyl)-2-hydroxybutanedioate Chemical compound [Na+].[Na+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O CEYULKASIQJZGP-UHFFFAOYSA-L 0.000 claims description 9
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 9
- 239000011790 ferrous sulphate Substances 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 9
- 238000009413 insulation Methods 0.000 claims description 9
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 9
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 9
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 9
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 9
- 235000002867 manganese chloride Nutrition 0.000 claims description 9
- 239000011565 manganese chloride Substances 0.000 claims description 9
- 229940099607 manganese chloride Drugs 0.000 claims description 9
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 9
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 9
- 238000010298 pulverizing process Methods 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- SEEPANYCNGTZFQ-UHFFFAOYSA-N sulfadiazine Chemical compound C1=CC(N)=CC=C1S(=O)(=O)NC1=NC=CC=N1 SEEPANYCNGTZFQ-UHFFFAOYSA-N 0.000 claims description 9
- 229960004306 sulfadiazine Drugs 0.000 claims description 9
- 238000010792 warming Methods 0.000 claims description 9
- 235000015099 wheat brans Nutrition 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 238000000034 method Methods 0.000 abstract description 4
- 238000009776 industrial production Methods 0.000 abstract description 3
- 238000004806 packaging method and process Methods 0.000 abstract 2
- 238000010924 continuous production Methods 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000007865 diluting Methods 0.000 abstract 1
- 239000003085 diluting agent Substances 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 238000011031 large-scale manufacturing process Methods 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 description 7
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 239000003910 polypeptide antibiotic agent Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 208000031295 Animal disease Diseases 0.000 description 1
- 108010001478 Bacitracin Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 244000057717 Streptococcus lactis Species 0.000 description 1
- 235000014897 Streptococcus lactis Nutrition 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229960003071 bacitracin Drugs 0.000 description 1
- 229930184125 bacitracin Natural products 0.000 description 1
- CLKOFPXJLQSYAH-ABRJDSQDSA-N bacitracin A Chemical compound C1SC([C@@H](N)[C@@H](C)CC)=N[C@@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]1C(=O)N[C@H](CCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2N=CNC=2)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)NCCCC1 CLKOFPXJLQSYAH-ABRJDSQDSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 244000005709 gut microbiome Species 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
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CN103053792A CN103053792A (zh) | 2013-04-24 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104054903A (zh) * | 2014-06-06 | 2014-09-24 | 李晓叶 | 一种发酵棉粕的生产工艺 |
CN106666091A (zh) * | 2016-12-11 | 2017-05-17 | 唐林元 | 一种肠溶型益生菌发酵酸化剂颗粒的制备方法 |
CN113249253A (zh) * | 2021-05-13 | 2021-08-13 | 内蒙古农业大学 | 一种复合益生菌的生产工艺 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1408760A2 (en) * | 2001-02-19 | 2004-04-21 | Société des Produits Nestlé S.A. | Consumable product containing probiotics |
CN1766095A (zh) * | 2005-09-16 | 2006-05-03 | 保罗生物园科技有限公司 | 一种益生菌复合酶的生产方法 |
CN101889631A (zh) * | 2010-07-07 | 2010-11-24 | 格特生物制药(天津)有限公司 | 一种辅料为山楂籽的畜禽用枯草芽孢杆菌、乳杆菌预混料及其制备方法 |
CN102356912A (zh) * | 2011-11-02 | 2012-02-22 | 黑龙江省龙蛙粮油进出口有限公司 | 一种益生菌发酵米乳的制备方法 |
CN102835555A (zh) * | 2011-06-21 | 2012-12-26 | 纽素乐必佳(天津)药业集团有限公司 | 复合益生菌预混料及其制备方法 |
-
2013
- 2013-01-09 CN CN201310007392.XA patent/CN103053792B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1408760A2 (en) * | 2001-02-19 | 2004-04-21 | Société des Produits Nestlé S.A. | Consumable product containing probiotics |
CN1766095A (zh) * | 2005-09-16 | 2006-05-03 | 保罗生物园科技有限公司 | 一种益生菌复合酶的生产方法 |
CN101889631A (zh) * | 2010-07-07 | 2010-11-24 | 格特生物制药(天津)有限公司 | 一种辅料为山楂籽的畜禽用枯草芽孢杆菌、乳杆菌预混料及其制备方法 |
CN102835555A (zh) * | 2011-06-21 | 2012-12-26 | 纽素乐必佳(天津)药业集团有限公司 | 复合益生菌预混料及其制备方法 |
CN102356912A (zh) * | 2011-11-02 | 2012-02-22 | 黑龙江省龙蛙粮油进出口有限公司 | 一种益生菌发酵米乳的制备方法 |
Non-Patent Citations (4)
Title |
---|
《含木聚糖酶复合益生菌饲料添加剂的研制及应用》;成珍等;《饲料与畜牧》;20120731(第7期);24-26页 * |
《活性益生菌饲料添加剂的开发》;郑德富等;《饲养饲料》;20100831(第8期);50-51页 * |
成珍等.《含木聚糖酶复合益生菌饲料添加剂的研制及应用》.《饲料与畜牧》.2012,(第7期),24-26页. |
郑德富等.《活性益生菌饲料添加剂的开发》.《饲养饲料》.2010,(第8期),50-51页. |
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Address after: Livillage Li Hai town 274916 administrative villages in Shandong province Juye County No. 076 Applicant after: Li Xiaoye Address before: 250132, room 3, unit 1, building 22, 201 Wang Dam Road, Licheng District, Shandong, Ji'nan Applicant before: Li Xiaoye |
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Inventor after: Wang Ye Inventor before: Li Xiaoye |
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Effective date of registration: 20170915 Address after: 510000 Guangdong city of Guangzhou province high tech Industrial Development Zone Ruitailu No. 2 building first layer B Patentee after: Guangzhou puwell sincessta Pharmaceutical Co. Ltd. Address before: Livillage Li Hai town 274916 administrative villages in Shandong province Juye County No. 076 Patentee before: Li Xiaoye |
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Inventor after: Wang Ye Inventor after: Liu Yangyang Inventor after: Wang Congfeng Inventor before: Wang Ye |