CN102919057A - Method for utilizing pine stump to plant Indian buead - Google Patents

Abstract

The invention provides a method for utilizing pine stump to plant Indian buead, which comprises the following steps: peeling, branch root cutting, seed selection, mycelium primers, inoculation, management, pestis prevention and cure and other steps. The method has the characteristics of simplicity in technology, low cost, quick effect and the like.

Description

A kind of method with pine tree root and stem of certain plants plantation Poria cocos

Technical field

The present invention relates to a kind of implantation methods of Chinese herbal medicine, relate in particular to a kind of implantation methods of Poria cocos.

Background technology

The Poria cocos nature and flavor are light flat, enter the heart, lung, the spleen channel.Has eliminating dampness and diuresis, strengthening the spleen and stomach, the effect of antitoxic heart-soothing and sedative.Can control difficult urination, the oedema turgor, phlegm and retained fluid is coughed contrary, vomits contrary, and vomiting during early pregnancy is had loose bowels, seminal emission, stranguria with turbid discharge, palpitation with fear, forgetful disease such as grade.The Li Shui of Poria cocos reaches by strong fortune spleen PFT, and is different from the Chinese medicine of other direct Li Shui.Linggui zhugan decoction, Sijunzi Tang, four Siberian cocklebur soup etc. all are prescriptions commonly used that the Poria cocos compatibility is arranged.Along with the development of China's herbal pharmaceutical industry, the demand of Poria cocos is increasing, and wild Poria cocos is difficult to the demand of satisfying the market.

Summary of the invention

Purpose of the present invention is exactly to provide a kind of artificial method with pine tree root and stem of certain plants plantation Poria cocos for above-mentioned situation.Purpose of the present invention can realize by following scheme: a kind of method with pine tree root and stem of certain plants plantation Poria cocos, and its feature may further comprise the steps: 1, peel, pine tree root and stem of certain plants exocuticle is eliminated; 2, cut lateral root, reveal in the winter time when shining butt, dig 20 centimetres of furrow widths of circle, the degree of depth and root at one meter of distance butt deeply fixed, lateral root is chopped off; 3, seed selection, select mycelia to draw, it is Poria cocos bacterial classification through artificial cultivation that described mycelia is drawn, with potato 200 grams, glucose 20 grams, agar 20 grams, 1000 milliliters in water is made medium, with potato decortication, section, put and add water boil half an hour in the pot, use the multilayer filtered through gauze, add agar and glucose in the filtrate, be sub-packed in the test tube after dissolving, make the test tube slant, the mouth of pipe is tampon beyond the Great Wall, through autoclaving, then under sterile working, be placed on the off-the-shelf test tube slant medium with the white Siberian cocklebur meat of disinfection inoculation pin scraping soya bean size, under 22 ℃ of-25 ℃ of conditions, cultivate, test tube slant, one all left and right sides can cover with white velvet-like mycelia, and female kind that Here it is is planted mother to enlarge again and cultivated, the bacterial classification that this expansion is cultivated is in original seed, the prescription of cultivating the original seed used medium is: clean pine sawdust 30-40 kilogram, 5 kilograms in wheat bran, 2 kilograms in brown sugar, 0.5 kilogram of land plaster, add water an amount of, prepare in 500 milliliters the wide-mouth bottle of packing into behind the material compacting, a hole at the bottom of reaching bottle is pricked by central authorities, cotton balls on the bottle plug, autoclaving is inoculated under aseptic condition after the cooling; 4, inoculation is when Clear and Bright to Slight Heat, with axe will winter, ready pine tree root and stem of certain plants top be chopped to 40 centimetres of side brachiums then, wide 10-20 centimetre, thick 3 centimetres new openning, bacterial classification is sticked, and earthing covered butt inoculation part, then sprinkled on every side about Furadan 100 grams of the ant of going out at butt to get final product, butt preferably places the paulownia material, in order to pass and to draw soon, also can be used as to mend kiln, butt is put the bacterial classification amount, take 30 centimetres of root and stem of certain plants diameters as criterion calculation, each root and stem of certain plants is put kinds 8 bottles; 5, management, general inspection in 7-15 days is once found to pass and is drawn after the inoculation, should be in time with bacterial classification or get to pass and draw material wood benefit kiln in the good kiln, in order to prevent rain drop erosion, the drain ditch has been dug in sloping periphery on the Siberian cocklebur field, and primary stage of inoculation prevents that people and animals from stepping on, in order to avoid introduction comes off, after inoculation survives, just enter the knot Siberian cocklebur phase to White Dew, the sclerotium growth is answered running check Siberian cocklebur field behind the knot Siberian cocklebur rapidly, find polygon ground, should in time earth up, otherwise, when excessive rainfall, water infiltrates and to cause the pest field to rot the Siberian cocklebur phenomenon on the Poria cocos, when the weather is cold and the ground is frozen, many blindings antifreeze, insulation is after the beginning of spring, pull down blocked up soil, impel the Poria cocos growth, must prevent that people and animals from stepping on, in order to avoid affect the Poria cocos growth; 6, pest control mainly is termite control, in case termite occurs, available moderate liquor is sprayed in the kiln or with it the termite, at once blinding.It is simple that the present invention has technique, and cost is low, the characteristics such as produce effects fast.

Claims (1)

1. method with pine tree root and stem of certain plants plantation Poria cocos, its feature may further comprise the steps: peel (1), and pine tree root and stem of certain plants exocuticle is eliminated; (2) cut lateral root, reveal in the winter time when shining butt, dig 20 centimetres of furrow widths of circle, the degree of depth and root at one meter of distance butt deeply fixed, lateral root is chopped off; (3) seed selection, select mycelia to draw, it is Poria cocos bacterial classification through artificial cultivation that described mycelia is drawn, with potato 200 grams, glucose 20 grams, agar 20 grams, 1000 milliliters in water is made medium, with potato decortication, section, put and add water boil half an hour in the pot, use the multilayer filtered through gauze, add agar and glucose in the filtrate, be sub-packed in the test tube after dissolving, make the test tube slant, the mouth of pipe is tampon beyond the Great Wall, through autoclaving, then under sterile working, be placed on the off-the-shelf test tube slant medium with the white Siberian cocklebur meat of disinfection inoculation pin scraping soya bean size, under 22 ℃ of-25 ℃ of conditions, cultivate, test tube slant, one all left and right sides can cover with white velvet-like mycelia, and female kind that Here it is is planted mother to enlarge again and cultivated, the bacterial classification that this expansion is cultivated is in original seed, the prescription of cultivating the original seed used medium is: clean pine sawdust 30-40 kilogram, 5 kilograms in wheat bran, 2 kilograms in brown sugar, 0.5 kilogram of land plaster, add water an amount of, prepare in 500 milliliters the wide-mouth bottle of packing into behind the material compacting, a hole at the bottom of reaching bottle is pricked by central authorities, cotton balls on the bottle plug, autoclaving is inoculated under aseptic condition after the cooling; (4) inoculation is when Clear and Bright to Slight Heat, with axe will winter, ready pine tree root and stem of certain plants top be chopped to 40 centimetres of side brachiums then, wide 10-20 centimetre, thick 3 centimetres new openning, bacterial classification is sticked, and earthing covered butt inoculation part, then sprinkled on every side about Furadan 100 grams of the ant of going out at butt to get final product, butt preferably places the paulownia material, in order to pass and to draw soon, also can be used as to mend kiln, butt is put the bacterial classification amount, take 30 centimetres of root and stem of certain plants diameters as criterion calculation, each root and stem of certain plants is put kinds 8 bottles; (5) management, general inspection in 7-15 days is once found to pass and is drawn after the inoculation, should be in time with bacterial classification or get to pass and draw material wood benefit kiln in the good kiln, in order to prevent rain drop erosion, the drain ditch has been dug in sloping periphery on the Siberian cocklebur field, and primary stage of inoculation prevents that people and animals from stepping on, in order to avoid introduction comes off, after inoculation survives, just enter the knot Siberian cocklebur phase to White Dew, the sclerotium growth is answered running check Siberian cocklebur field behind the knot Siberian cocklebur rapidly, find polygon ground, should in time earth up, otherwise, when excessive rainfall, water infiltrates and to cause the pest field to rot the Siberian cocklebur phenomenon on the Poria cocos, when the weather is cold and the ground is frozen, many blindings antifreeze, insulation is after the beginning of spring, pull down blocked up soil, impel the Poria cocos growth, must prevent that people and animals from stepping on, in order to avoid affect the Poria cocos growth; (6) pest control mainly is termite control, in case termite occurs, available moderate liquor is sprayed in the kiln or with it the termite, at once blinding.