CN109761687A - A kind of liquid fertilizer and preparation method thereof improving Quality of Tomato Fruit - Google Patents

Abstract

The invention discloses a kind of liquid fertilizers and preparation method thereof for improving Quality of Tomato Fruit.The liquid fertilizer is by 1000 parts of active liquid fertilizer substrate preparation；26 ~ 42 parts of nitrogen, 36 ~ 68 parts of potassium, 6 ~ 14 parts of phosphorus, 4 ~ 10 parts of boron, 6 ~ 14 parts of calcium, 2 ~ 10 parts of magnesium；Contain 9 ~ 18 parts of proline, 14 ~ 26 parts of glycine, 15 ~ 23 parts of glutamic acid, 4 ~ 14 parts of aspartic acid, 6 ~ 18 parts of tryptophan, 1 ~ 6 part of alanine in the liquid fertilizer；Total nitrogen content >=2.5mg/g, amino nitrogen content >=0.4mg/g, the content of organic matter are 90 ~ 155g/L.The liquid fertilizer, which increases the fruit diameter growth rate, per mu yield and fruit quality of tomato, has remarkable effect.

Description

A kind of liquid fertilizer and preparation method thereof improving Quality of Tomato Fruit

Technical field

The invention belongs to the technical fields of liquid fertilizer, more particularly, to a kind of liquid for improving Quality of Tomato Fruit Fertilizer and preparation method thereof.

Background technique

Tomato is also known as tomato, and tomato is annual herb plant, and 0.6~2 meter of plant height, the raw cement glandular hairs of entirety have strong Strong smell.Inflorescence always obstructs 2~5 centimetres long, normal 3~7 flowers；Bennet is 1~1.5 centimetre long；Corolla is yellow spoke shape, diameter about 2 Centimetre.Berry oblate spheroid shape is close spherical, meat and many juice, is in orange colour or cerise, surface is smooth, and seed is yellow.Flower Fruiting period is summer and autumn.

Streptomyces (streptomyces) is a category for Actinomycetal.Substrate mycelium is not broken, and aerial hyphae usually sends out Educate good, the fibrillae of spores of formation long (sometimes short).Spore cannot move, and often have the shapes jewelry such as wart, thorn or hair on epitheca.Strepto- Bacterium is widely present in soil, is Filamentous gram-positive bacterium, mainly by sporogenesis, most streptomycetes are to people Body is harmless.Streptomycete is the main source for generating various antibiotic, the antibiotic derived from streptomycete include erythromycin, tetracycline, Streptomysin, chloramphenicol, neomycin, nystatin, kanamycins, cycloheximide and anphotericin etc., therefore, for streptomycete Existing research focuses primarily upon bacterial strain and generates ability and size of antibacterial substance etc..

The phenomenon and growth period fruit drop phenomenon that blooming less often occurs in tomato, result is few etc..Except tomato itself Outside hereditary capacity, mainly since the shortage of element needed for growing in cultivation leads to the quality of bud, the development of floral organ Situation is obstructed.

Summary of the invention

The technical problem to be solved by the present invention is to overcome existing tomato occur result is few, fruit is small and growth period fallen flowers The problem of shedding, provides a kind of liquid fertilizer and preparation method thereof for improving Quality of Tomato Fruit, during tomato cultivation Using.

Above-mentioned purpose of the present invention is achieved through the following technical solutions: a kind of liquid fertilizer improving Quality of Tomato Fruit, by The material composition of following parts by weight:

1000 parts of active liquid fertilizer substrate preparation；26~42 parts of nitrogen, 36~68 parts of potassium, 6~14 parts of phosphorus, 4~10 parts of boron, 6~14 parts of calcium, 2~10 parts of magnesium；Contain 9~18 parts of proline, 14~26 parts of glycine, glutamic acid 15 in the liquid fertilizer ~23 parts, 4~14 parts of aspartic acid, 6~18 parts of tryptophan, 1~6 part of alanine；Total nitrogen content >=2.5mg/g, ammonia nitrogen contain Amount >=0.4mg/g, the content of organic matter are 90~155g/L.

The active liquid fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation；Streptomyces bacterial strain The culture medium of " TY-001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, be beaten, filtered；Then toward filter After sugar is added in liquid, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；The streptomyces bacterium Strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, and classification naming is Streptomyces., deposit number is GDMCC No.:60152, and preservation address is Xianlie Mid Road, Guangzhou City, Guangdong Province, China No. 100 5 building, the buildings of compound the 59th.

Further, described 1000 parts of active liquid fertilizer substrate preparation；28~36 parts of nitrogen, 46~58 parts of potassium, phosphorus 8~12 Part, 6~9 parts of boron, 8~12 parts of calcium, 5~8 parts of magnesium.

Further, in the liquid fertilizer containing 11~16 parts of proline, 18~22 parts of glycine, glutamic acid 17~ 20 parts, 6~10 parts of aspartic acid, 11~13 parts of tryptophan, 2~4 parts of alanine；Total nitrogen content >=2.5mg/g, amino nitrogen content >=0.4mg/g, the content of organic matter are 110~142g/L.

Preferably, it is described improve Quality of Tomato Fruit liquid fertilizer by following parts by weight material composition:

1000 parts of active liquid fertilizer substrate preparation；31 parts of nitrogen, 50 parts of potassium, 10 parts of phosphorus, 7 parts of boron, 10 parts of calcium, 7 parts of magnesium；Institute It states and contains 14 parts of proline, 20 parts of glycine, 18 parts of glutamic acid, 8 parts of aspartic acid, 12 parts of tryptophan, the third ammonia in liquid fertilizer 4 parts of acid；Total nitrogen content >=2.5mg/g, amino nitrogen content >=0.4mg/g, content of organic matter 120g/L.

Further, the active liquid fertilizer substrate preparation is prepared by the following steps to obtain:

S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then past After sugar is added in filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；

S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, the continuing fermentation 7~8 at 20~28 DEG C After it, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；

The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017 The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is that Guangdong province, China is wide 5 building, the building of compound the 59th of state city martyr Road 100.

Preferably, the pre-fermented step are as follows:

S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e. It can；

S12. it is covered on dregs of beans or dregs of beans surface with film, left no gaps, timing starts pre fermentation process；

S13. the pre fermentation time is 3~5 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, in fermentation raw material When portion temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continue fermentation 2~3 days.

Preferably, in the liquid spawn every milliliter have 200,000,000 viable bacterias；The ratio of the liquid spawn and culture medium is 165g:250L。

Preferably, the ratio between the active liquid fertilizer substrate preparation nitrogen and potassium are 1:1.5~1.8.

Preferably, the ratio between the aspartic acid, tryptophan, alanine are 2:3:1.

Preferably, its amino-acid nitrogen content >=4.5g/L after the active liquid fertilizer substrate preparation is stored 15 days.

Preferably, adding water to be settled to concentration sugared in culture medium in the step S1 is 100g/L.

A kind of preparation method of the liquid fertilizer improving Quality of Tomato Fruit, comprising the following steps:

S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then past After sugar is added in filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；

S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, the continuing fermentation 7~8 at 20~28 DEG C After it, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；

S5. proline, glutamic acid, glycine, third configuration of liquid fertilizer: are added toward the active liquid fertilizer substrate preparation One or more of propylhomoserin, aspartic acid, tryptophan or nitrogen, phosphorus, potassium, boron, calcium, magnesium obtain the liquid fertilizer；The chain Mould category bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, and classification naming is Streptomyces., deposit number is GDMCC No.:60152, and preservation address is Xianlie Mid Road, Guangzhou City, Guangdong Province, China No. 100 5 building, the buildings of compound the 59th.

Preferably, the sugar in the step S1 is one or more of white granulated sugar, sucrose or brown sugar.

Preferably, the morphological feature of streptomyces bacterial strain " TY-001# " are as follows: bacterium colony is rounded, milky, the smooth of the edge, Show there is condensed milk shape gloss；Spore Gram's staining is positive.

A kind of strain cultivation method of the streptomyces bacterial strain (Streptomyces), comprising the following steps:

S21. streptomyces bacterial strain " TY-001# " is taken to line on solid medium, 25~30 DEG C of inversion activation cultures 3~ 7 days, bacterial strain after activation is made；

S22. strain inoculated is 25~30 DEG C, revolution in temperature into fluid nutrient medium after taking step S1 activation obtained Under conditions of 150~220 revs/min, shaking table culture 3~7 days, bacterium solution is obtained.

Preferably, every liter of component of the fluid nutrient medium is as follows: soybean, dregs of beans 100g being taken to wear into soya-bean milk filtering, waste；It is white Granulated sugar, sucrose or brown sugar 100g；Water is settled to 1 liter, and pH is 7.4~7.6.

Preferably, every liter of component of the fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, Water is settled to 1 liter, and pH is 7.4~7.6.

Preferably, every liter of component of the solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, 15~25g of agar, water are settled to 1 liter, and pH is 7.4~7.6.

Compared with prior art, the invention has the following advantages:

Liquid fertilizer of the invention is capable of the fruit quality of conspicuousness raising tomato, can quickly supplement needed for tomato growth Nutrient, promote flowers and fruits growth, be conducive to the growth and development of plant.The tamato fruit transverse diameter for applying liquid fertilizer of the invention increases Long rate is up to 248.0%, has been higher by 98% than control group, and single fresh fruit also increases 5% again, realizes that per mu yield growth rate is high Up to 71%, which, which increases the fruit diameter growth rate, per mu yield and fruit quality of tomato, has remarkable effect, and effect is very It is considerable, it improves a lot to the fruit quality of tomato, soluble solid content increases 39.3%.As it can be seen that of the invention Liquid fertilizer has very significant effect to the quality of tomato.Streptomyces bacterial strain " TY-001# " provided by the invention, the bacterium Strain can adapt to aerobic fementation culture under hyperosmosis.

Specific embodiment

Further illustrate the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of It limits.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method and apparatus. Unless stated otherwise, following embodiment agents useful for same and material are commercially available.

The separation and identification of 1 streptomyces bacterial strain of embodiment (Streptomyces)

1. experimental material and condition

(1) in the collected pedotheque in Hunan Province Chenzhou City somewhere.

(2) every liter of component of fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, water are settled to 1 It rises, pH 7.5.

Every liter of component of solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, agar 15~ 25g, water are settled to 1 liter, pH 7.5.

Culture medium packing, high-pressure sterilizing pot sterilize (121 DEG C, 30min).

(3) aseptic technique: all vessel and apparatus must sterilize (121 DEG C, 30min) through high-pressure sterilizing pot, inoculation Equal operations carry out in superclean bench.

(4) strain culturing condition: being placed in culture in 25 ± 1 DEG C of illumination insulating boxs (14L:10D), after bacterium colony is formed, turns The inclined-plane PDA is moved on to, then is transferred in 5 DEG C of refrigerators and saves.

2. strain isolation and screening

(1) separation obtains a streptomycete category bacterial strain T0 from the pedotheque in Hunan Province Chenzhou City somewhere.

(2) by inclined-plane squamous subculture in isolated streptomyces bacterial strain T0 access solid medium, then 5 DEG C of ice are transferred to It is saved in case.

(3) by the bacterial strain T0 of squamous subculture in fluid nutrient medium, 220 revs/min on oscillation shaking table, 25 DEG C of conditions Lower culture 5 days.

(4) the T0 liquid bacteria after cultivating 5 days, is placed at 20 watts of ultraviolet radiator 25cm and irradiates 2 hours.

(5) it will be cultivated 5 days in the T0 liquid bacteria access solid slope culture medium after irradiation 25 DEG C, then choose and grow most Good surviving colonies access solid medium squamous subculture.

(6) several processes all over step (2)~(5) are repeated to recycle.Each in repetitive process circulation, solid medium All increase by 1 gram with the sodium chloride dosage in fluid nutrient medium, until the sodium chloride dosage in solid medium and fluid nutrient medium Reach 25 grams.The healthy and strong bacterial strain finally survived is subjected to squamous subculture, then is transferred in 5 DEG C of refrigerators and saves.

(7) the osmophilic strain bacterial strain T0 that will be filtered out above carries out fermentation production run.Through 7 days 25 DEG C open wide it is aerobic After fermentation, amino nitrogen in fermentation liquid (measuring ammonia nitrogen standard detection by formol titration) is measured, amino nitrogen is picked out and contains (>=0.7mg/L) highest bacterial strain is measured as final production bacterial strain, is named " TY-001# ".

Culture medium used in fermentation production run is as follows: taking soybean 100g to wear into soya-bean milk filtering, abandons bean dregs；White granulated sugar 100g is settled to 1000mL, pH 7.5.

3. the identification of bacterial strain " TY-001# "

(1) Morphological Identification

Morphological feature are as follows: bacterium colony is rounded, milky, the smooth of the edge, shows there is condensed milk shape gloss；Spore Gram's staining It is positive.

Morphological Identification shows that the bacterial strain belongs to streptomyces.

In conclusion bacterial strain " TY-001# " is streptomyces, Guangdong Province's microbial bacteria is preserved on March 24th, 2017 Kind collection, deposit number are GDMCC No.:60152, and preservation address is Xianlie Mid Road, Guangzhou City, Guangdong Province, China 100 Number 5 building, the building of compound the 59th.

The embodiment 2 a kind of preparation and application of the liquid fertilizer for improving Quality of Tomato Fruit

It is a kind of improve Quality of Tomato Fruit liquid fertilizer preparation method the following steps are included:

S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；

S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.

S5. the configuration of liquid fertilizer: toward 1000 parts of active liquid fertilizer substrate preparations addition proline, glycine, glutamic acid, Aspartic acid, tryptophan, alanine, nitrogen, phosphorus, potassium, boron, calcium, magnesium, obtain liquid fertilizer；

Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city 5 building, the building of compound the 59th of martyr Road 100.

The liquid fertilizer of Quality of Tomato Fruit is improved by the material composition of following parts by weight:

1000 parts of active liquid fertilizer substrate preparation；31 parts of nitrogen, 50 parts of potassium, 10 parts of phosphorus, 7 parts of boron, 10 parts of calcium, 7 parts of magnesium；

In liquid fertilizer contain 14 parts of proline, 20 parts of glycine, 18 parts of glutamic acid, 8 parts of aspartic acid, tryptophan 12 Part, 4 parts of alanine；Total nitrogen content >=2.5mg/g, amino nitrogen content >=0.4mg/g, content of organic matter 120g/L.Wherein N, P, K, Ca, B, Mg are respectively derived from ammonium dihydrogen phosphate and amino acid, ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, sulphur Sour magnesium.

The embodiment 3 a kind of preparation and application of the liquid fertilizer for improving Quality of Tomato Fruit

It is a kind of improve Quality of Tomato Fruit liquid fertilizer preparation method the following steps are included:

S1. it prepares culture medium: after taking 1000g dregs of beans to carry out pre fermentation, being put into fiberizer and be beaten, filter, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

Pre-fermented step are as follows:

S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e. It can；

S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；

S13. the pre fermentation time is 4 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, temperature inside fermentation raw material At >=40 DEG C of degree, pre fermentation dregs of beans is inside and outside overturn once, continues fermentation 2 days.

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；

S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.

S5. the configuration of liquid fertilizer:

Proline, glycine, glutamic acid, aspartic acid, tryptophan, third are added toward 1000 parts of active liquid fertilizer substrate preparations Propylhomoserin, nitrogen, phosphorus, potassium, boron, calcium, magnesium, obtain liquid fertilizer；

Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city 5 building, the building of compound the 59th of martyr Road 100.

The liquid fertilizer of Quality of Tomato Fruit is improved by the material composition of following parts by weight:

1000 parts of active liquid fertilizer substrate preparation；36 parts of nitrogen, 58 parts of potassium, 12 parts of phosphorus, 9 parts of boron, 12 parts of calcium, 8 parts of magnesium；Liquid Contain 16 parts of proline, 22 parts of glycine, 20 parts of glutamic acid, 10 parts of aspartic acid, 15 parts of tryptophan, alanine 5 in body fertilizer Part；, total nitrogen content >=2.5mg/g, amino nitrogen content >=0.4mg/g, content of organic matter 142g/L.Wherein N, P, K, Ca, B, Mg It is respectively derived from ammonium dihydrogen phosphate and amino acid, ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, magnesium sulfate.

The embodiment 4 a kind of preparation and application of the liquid fertilizer for improving Quality of Tomato Fruit

It is a kind of improve Quality of Tomato Fruit liquid fertilizer preparation method the following steps are included:

S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L

S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.

S5. the configuration of liquid fertilizer: toward 1000 parts of active liquid fertilizer substrate preparations addition proline, glycine, glutamic acid, Aspartic acid, tryptophan, alanine, nitrogen, phosphorus, potassium, boron, calcium, magnesium, obtain liquid fertilizer；

Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city 5 building, the building of compound the 59th of martyr Road 100.

The liquid fertilizer of Quality of Tomato Fruit is improved by the material composition of following parts by weight:

1000 parts of active liquid fertilizer substrate preparation；26 parts of nitrogen, 39 parts of potassium, 6 parts of phosphorus, 4 parts of boron, 6 parts of calcium, 2 parts of magnesium；

In liquid fertilizer containing 9 parts of proline, 14 parts of glycine, 15 parts of glutamic acid, 4 parts of aspartic acid, 6 parts of tryptophan, 2 parts of alanine；Total nitrogen content >=2.5mg/g, amino nitrogen content >=0.4mg/g, content of organic matter 90g/L.Wherein N, P, K, Ca, B, Mg are respectively derived from ammonium dihydrogen phosphate and amino acid, ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, magnesium sulfate.

The embodiment 5 a kind of preparation and application of the liquid fertilizer for improving Quality of Tomato Fruit

It is a kind of improve Quality of Tomato Fruit liquid fertilizer preparation method the following steps are included:

S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；

S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.

S5. the configuration of liquid fertilizer: toward 1000 parts of active liquid fertilizer substrate preparations addition proline, glycine, glutamic acid, Aspartic acid, tryptophan, alanine, nitrogen, phosphorus, potassium, boron, calcium, magnesium, obtain liquid fertilizer；

Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city 5 building, the building of compound the 59th of martyr Road 100.

The liquid fertilizer of Quality of Tomato Fruit is improved by the material composition of following parts by weight:

1000 parts of active liquid fertilizer substrate preparation；42 parts of nitrogen, 68 parts of potassium, 14 parts of phosphorus, 10 parts of boron, 14 parts of calcium, 10 parts of magnesium；

Contain 18 parts of proline, 26 parts of glycine, 23 parts of glutamic acid, 12 parts of aspartic acid, tryptophan 18 in liquid fertilizer Part, 6 parts of alanine；Total nitrogen content >=2.5mg/g, amino nitrogen content >=0.4mg/g, content of organic matter 155g/L.Wherein N, P, K, Ca, B, Mg are respectively derived from ammonium dihydrogen phosphate and amino acid, ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, sulphur Sour magnesium.

The comparative example 1 a kind of preparation and application of the liquid fertilizer for improving Quality of Tomato Fruit

It is a kind of improve Quality of Tomato Fruit liquid fertilizer preparation method the following steps are included:

S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

S2. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S3. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation.

S4. the configuration of liquid fertilizer: toward 1000 parts of active liquid fertilizer substrate preparations addition proline, glycine, glutamic acid, Aspartic acid, tryptophan, alanine, nitrogen, phosphorus, potassium, boron, calcium, magnesium obtain the liquid fertilizer；

The liquid fertilizer of Quality of Tomato Fruit is improved by the material composition of following parts by weight:

1000 parts of active liquid fertilizer substrate preparation；26 parts of nitrogen, 39 parts of potassium, 6 parts of phosphorus, 4 parts of boron, 6 parts of calcium, 2 parts of magnesium；

In liquid fertilizer containing 9 parts of proline, 14 parts of glycine, 15 parts of glutamic acid, 4 parts of aspartic acid, 6 parts of tryptophan, 2 parts of alanine；Total nitrogen content >=2.5mg/g, amino nitrogen content >=0.4mg/g, content of organic matter 90g/L.Wherein N, P, K, Ca, B, Mg are respectively derived from ammonium dihydrogen phosphate and amino acid, ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, magnesium sulfate.

Improve the application effect appraisal of the liquid fertilizer of Quality of Tomato Fruit:

Nutrient needed for liquid fertilizer can quickly supplement tomato growth promotes flowers and fruits growth.It is prepared by the present invention to verify The liquid fertilizer actual effect that large-scale demonstration is applied on tomato, applicant's selection have carried out experiment and demonstration on tomato.

1 materials and methods

1.1 are located in Zixing City tomato base for examination place, and 10 mu of testing ground area, soil fertility level is more uniform.

1.2 for studying object tomato, seeding row spacing 40cm*40cm, the growing way golden mean of the Confucian school and consistent.

1.3 liquid fertilizer for trying to improve Quality of Tomato Fruit made from fertilizer embodiment 2~5, product forms are liquid.

1.4 carry out 3 test process for examination place:

1. experimental group: for examination fertilizer in be colonized in latter week pour apply it is primary, 500mL/ mu, 100 times of dilution；Xie Huahou is sprayed Once, 300mL/ mus, 100 times are diluted；First fringe fruit is poured when starting to expand applies 700mL/ mus, dilutes 100 times, poured and apply every 10 days Once, it pours and applies twice.

2. control group (CK): per acre every time with the progress of same experimental group equivalent clear water same time, other measures of fertilizer are the same as processing ①。

3. comparative example group: being carried out every time with the chemical fertilizer same time prepared by the comparative example 1 of same experimental group equivalent per acre, other are applied The same processing of fertile measure is 1..

Experimental group and control group, comparative example group are managed by local conventional cultivation technology.Each processing is tested with per acre It for cell, is repeated 3 times, random alignment, management is consistent.Determined before and after the picking time at random 5 plants of plant carry out fixed point investigation east, West, south, north, in the average value of all fruit transverse diameters on 5 plant and being averaged for single plant fruiting number and single fresh fruit weight Value calculates growth rate.Count per mu yield, each processing fruit quality of detection.

2 results and analysis

The influence of 2.1 pairs of fruit transverse diameters

As shown in Table 1, the tamato fruit transverse diameter growth rate for applying the liquid fertilizer is up to 248.0%, is higher by than control group 98%.

Table 1

The influence of 2.2 pairs of per mu yields

Strain number, random 5 points of samplings, every points complete stool fruiting number claim fresh fruit weight, calculate single plant fruiting number, list statistics per acre The average value of a fresh fruit weight, calculates theoretical per mu yield.

Through calculating, 4092 plants of tomato trees, the average value such as table of single plant fruiting number, single fresh fruit weight and per mu yield are planted per acre 2.As shown in Table 2, used the tomato fruit retention rate of the liquid fertilizer up to 87.5%, and control group is only 80%；Single fresh fruit weight 5% is also increased, realizes that per mu yield growth rate is up to 71%, effect is considerable.

Table 2

Processing	Single plant fruiting number/	Single fresh fruit weight/kg	Per mu yield/kg
				Tomato experimental group	7	0.21	6015
Tomato control group	4	0.2	3503
				Tomato comparative example group	5	0.2	4902

The influence of 2.3 pairs of soluble solid contents

It squeezes the juice after taking several fresh fruits to remove the peel at random, measures soluble fixture content, experimental group and control group, comparative example Each measurement of group 5 times, is averaged.Experimental result is as shown in table 3.

As shown in Table 3, which improves a lot to the fruit quality of tomato, and soluble solid content increases 39.3%.

Table 3

Processing	Soluble solid content/%
		Tomato experimental group	21.6
Tomato control group	15.5
		Tomato comparative example group	17.1

Claims (10)

1. a kind of liquid fertilizer for improving Quality of Tomato Fruit, which is characterized in that by the material composition of following parts by weight:

1000 parts of active liquid fertilizer substrate preparation；26~42 parts of nitrogen, 36~68 parts of potassium, 6~14 parts of phosphorus, 4~10 parts of boron, calcium 6~ 14 parts, 2~10 parts of magnesium；

In the liquid fertilizer containing 9~18 parts of proline, 14~26 parts of glycine, 15~23 parts of glutamic acid, aspartic acid 4~ 14 parts, 6~18 parts of tryptophan, 1~6 part of alanine；Total nitrogen content >=2.5mg/g, amino nitrogen content >=0.4mg/g, organic matter Content is 90~155g/L；

The active liquid fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation；Streptomyces bacterial strain " TY- The culture medium of 001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, be beaten, filtered；Then into filtrate After sugar is added, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；Streptomyces bacterial strain " the TY- 001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, classification naming Streptomyces., Deposit number is GDMCC No.:60152, and preservation address is No. 59 building 5 of compound of Xianlie Mid Road, Guangzhou City, Guangdong Province, China 100 Building.

2. improving the liquid fertilizer of Quality of Tomato Fruit according to claim 1, which is characterized in that by the object of following parts by weight Matter composition:

1000 parts of active liquid fertilizer substrate preparation；28~36 parts of nitrogen, 46~58 parts of potassium, 8~12 parts of phosphorus, 6~9 parts of boron, calcium 8~12 Part, 5~8 parts of magnesium；

Contain 11~16 parts of proline, 18~22 parts of glycine, 17~20 parts of glutamic acid, aspartic acid 6 in the liquid fertilizer ~10 parts, 11~13 parts of tryptophan, 2~4 parts of alanine；Total nitrogen content >=2.5mg/g, amino nitrogen content >=0.4mg/g are organic Matter content is 110~142g/L.

3. improving the liquid fertilizer of Quality of Tomato Fruit according to claim 1, which is characterized in that by the object of following parts by weight Matter composition:

1000 parts of active liquid fertilizer substrate preparation；31 parts of nitrogen, 50 parts of potassium, 10 parts of phosphorus, 7 parts of boron, 10 parts of calcium, 7 parts of magnesium；

Contain 14 parts of proline, 20 parts of glycine, 18 parts of glutamic acid, 8 parts of aspartic acid, tryptophan 12 in the liquid fertilizer Part, 4 parts of alanine；Total nitrogen content >=2.5mg/g, amino nitrogen content >=0.4mg/g, content of organic matter 120g/L.

4. improving the liquid fertilizer of Quality of Tomato Fruit according to claim 1, which is characterized in that the active liquid fertilizer base Bottom preparation is prepared by the following steps to obtain:

S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then toward filtrate After middle addition sugar, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；

S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, at 20~28 DEG C after continuing fermentation 7~8 days, Obtain fermentation liquid；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；

The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city 5 building, the building of compound the 59th of martyr Road 100.

5. improving the liquid fertilizer of Quality of Tomato Fruit according to claim 1, which is characterized in that the pre-fermented step Are as follows:

S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans clenching of the hand reaches the state for dripping into line；

S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；

S13. the pre fermentation time is 3~5 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, temperature inside fermentation raw material At >=40 DEG C of degree, pre fermentation dregs of beans is inside and outside overturn once, continue fermentation 2~3 days.

6. improving the liquid fertilizer of Quality of Tomato Fruit according to claim 1, which is characterized in that the active liquid fertilizer base The ratio between bottom preparation nitrogen and potassium are 1:1.5~1.8.

7. improving the liquid fertilizer of Quality of Tomato Fruit according to claim 1, which is characterized in that the aspartic acid, color The ratio between propylhomoserin, alanine are 2:3:1.

8. improving the liquid fertilizer of Quality of Tomato Fruit according to claim 1, which is characterized in that the active liquid fertilizer base Its amino-acid nitrogen content >=4.5g/L after bottom preparation is stored 15 days.

9. a kind of preparation method for the liquid fertilizer for improving Quality of Tomato Fruit, which comprises the following steps:

S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then toward filtrate After middle addition sugar, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；

S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, at 20~28 DEG C after continuing fermentation 7~8 days, Obtain fermentation liquid；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；

S5. proline, glutamic acid, glycine, the third ammonia the configuration of liquid fertilizer: are added toward the active liquid fertilizer substrate preparation One or more of acid, aspartic acid, tryptophan or nitrogen, phosphorus, potassium, boron, calcium, magnesium obtain the liquid fertilizer；

The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city 5 building, the building of compound the 59th of martyr Road 100.

10. application of claim 1~9 liquid fertilizer for improving Quality of Tomato Fruit during tomato cultivation.