CN109956813A - A kind of liquid fertilizer and preparation method thereof improving fruit corn fruit quality - Google Patents
A kind of liquid fertilizer and preparation method thereof improving fruit corn fruit quality Download PDFInfo
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- CN109956813A CN109956813A CN201711427364.8A CN201711427364A CN109956813A CN 109956813 A CN109956813 A CN 109956813A CN 201711427364 A CN201711427364 A CN 201711427364A CN 109956813 A CN109956813 A CN 109956813A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a kind of liquid fertilizers and preparation method thereof for improving fruit corn fruit quality, which includes 1000 parts of active liquid fertilizer substrate preparation, 0.1 ~ 3 part of chitosan oligosaccharide, 62 ~ 191 parts of amino acid 1,2 ~ 10 parts of potassium sulfate, 52 ~ 68 parts of calcium superphosphate, 5 ~ 18 parts of boric acid, 1 ~ 8 part of calcium gluconate, 3 ~ 12 parts of white vitriol；The liquid fertilizer not only effectively increases the fruit quality of fruit corn, increases fruit yield, while having selectivity to the effect of different corns, the most significant to the effect of fruit corn.
Description
Technical field
The invention belongs to the technical fields of liquid fertilizer, more particularly, to a kind of raising fruit corn fruit quality
Liquid fertilizer and preparation method thereof.
Background technique
Fruit corn is a kind of super-sweet corn for being suitble to eat it raw, and green stick stage thin skin, juice is more, matter is crisp and sweet tea, can directly be given birth to
Eat, for very thin epidermis once stinging brokenly, the juice of faint scent overflows tooth cheek, eat it raw it is ripe eat all special sweet tea, especially crisp, as fruit,
Therefore it is referred to as " fruit corn ".Fruit corn belongs to one of corn, is the developed countries such as American-European, South Korea and Japan
One of main vegetables, because that it is with nutrition abundant, sweet tea, fresh, crisp, tender characteristic is deep by each stratum consumers.Production
In corn can be divided into conventional corn, super-sweet corn and reinforced corn three classes, super-sweet corn is high, suitable due to sugar content
Suitable picking time is long and is planted extensively.Corn also has cultivation in China.Plant is shorter, and tillering ability is stronger.On fruit ear bract
There is boot leaf.Seed is yellowish or white, and embryo is larger, and milk stage is tender in fact, is rich in water-soluble polysaccharide, vitamin A, vitamin C, rouge
Fat and protein etc..
Streptomyces (streptomyces) is a category for Actinomycetal.Substrate mycelium is not broken, and aerial hyphae usually sends out
Educate good, the fibrillae of spores of formation long (sometimes short).Spore cannot move, and often have the shapes jewelry such as wart, thorn or hair on epitheca.Strepto-
Bacterium is widely present in soil, is Filamentous gram-positive bacterium, mainly by sporogenesis, most streptomycetes are to people
Body is harmless.Streptomycete is the main source for generating various antibiotic, the antibiotic derived from streptomycete include erythromycin, tetracycline,
Streptomysin, chloramphenicol, neomycin, nystatin, kanamycins, cycloheximide and anphotericin etc., therefore, for streptomycete
Existing research focuses primarily upon bacterial strain and generates ability and size of antibacterial substance etc..
Partially to fertilize, especially inclined nitrogen fertilizer application easily lacks water along with land occupation condition is poor for traditional fertilising habit happiness
The elements such as the big phosphorus potassium calcium of fruit corn plants body demand, are easier to cause the shortage of microelement, influence plant and normally grow hair
It educates, causes low nutriment contained by fruit corn, inane fruit, suppresses fruit, seriously affected corn yield.
Summary of the invention
Small, inane fruit that the technical problem to be solved by the present invention is to overcome existing fruit corn fruits suppresses fruit, contained nutriment
Less, the problem of low output, poor taste, a kind of liquid fertilizer and preparation method thereof for improving fruit corn fruit quality is provided.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of liquid fertilizer improving fruit corn fruit quality, by the material composition of following parts by weight:
1000 parts of active liquid fertilizer substrate preparation, 0.1~3 part of chitosan oligosaccharide, 62~191 parts of amino acid 1, potassium sulfate 2~10
Part, 52~68 parts of calcium superphosphate, 5~18 parts of boric acid, 1~8 part of calcium gluconate, 3~12 parts of white vitriol；The Liquid Fertilizer
Total nitrogen content >=2.5mg/g in material, amino nitrogen content >=0.4mg/g, the content of organic matter are 110~165g/L.The activity liquid
Body fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation；The culture medium of streptomyces bacterial strain " TY-001# " are as follows:
After taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, it is beaten, is filtered；Then after sugar being added into filtrate, add water fixed
Hold the concentration >=100g/L sugared into culture medium, pH is 7.4~7.6；
The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017
The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is that Guangdong province, China is wide
State city martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
Preferably, the liquid fertilizer is by following material composition: 1000 parts of active liquid fertilizer substrate preparation,
0.5~1.5 part of chitosan oligosaccharide, 70~178 parts of amino acid 1,3~8 parts of potassium sulfate, 56~62 parts of calcium superphosphate, boric acid 8
~12 parts, 3~5 parts of calcium gluconate, 6~9 parts of white vitriol；Total nitrogen content >=2.5mg/g, amino in the liquid fertilizer
Nitrogen content >=0.4mg/g, the content of organic matter are 115~130g/L.
Preferably, the liquid fertilizer is by following material composition: 1000 parts of active liquid fertilizer substrate preparation, 1 part of chitosan oligosaccharide,
75 parts of amino acid 1,5 parts of potassium sulfate, 60 parts of calcium superphosphate, 10 parts of boric acid, 3 parts of calcium gluconate, 7 parts of white vitriol；It is described
Total nitrogen content >=2.5mg/g in liquid fertilizer, amino nitrogen content >=0.4mg/g, the content of organic matter are 119~126g/L.
Further, the weight ratio of N, P, K are 25~32:12~18:1, preferably 28:16:1 in the liquid fertilizer.
Further, the mixing that the amino acid is poly- asparagus fern door propylhomoserin, lysine, tryptophan are prepared in 1:1:1 ratio
Aminoacids solution.
Further, 3~12 parts of 5~18 parts of the boric acid, 1~8 part of calcium gluconate, white vitriol each component ratios
For 2~5:2:3~6.
A kind of preparation method of the liquid fertilizer improving fruit corn fruit quality, comprising the following steps:
S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then past
After sugar is added in filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, continuing fermentation 7~8 days at 20~28 DEG C
Afterwards, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；
S5. chitosan oligosaccharide, amino acid, potassium sulfate, mistake the configuration of liquid fertilizer: are added toward the active liquid fertilizer substrate preparation
Calcium phosphate, boric acid, calcium gluconate, white vitriol, selenite obtain the liquid fertilizer.
Further, the pre-fermented step are as follows:
S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e.
It can；
S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；
S13. the pre fermentation time is 3~5 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, in fermentation raw material
When portion temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continue fermentation 2~3 days.
Preferably, adding water to be settled to concentration sugared in culture medium in the step S1 is 100g/L.
Preferably, its amino-acid nitrogen content >=4.5g/L after the active liquid fertilizer substrate preparation is stored 15 days.
Preferably, in the liquid spawn every milliliter have 200,000,000 viable bacterias；The ratio of the liquid spawn and culture medium is
165g:250L。
Preferably, the morphological feature of streptomyces bacterial strain " TY-001# " are as follows: bacterium colony is rounded, milky, the smooth of the edge,
Show there is condensed milk shape gloss；Spore Gram's staining is positive.
A kind of cultural method of the streptomyces bacterial strain (Streptomyces), comprising the following steps:
Y1. streptomyces bacterial strain " TY-001# " is taken to line on solid medium, 25~30 DEG C of inversion activation cultures 3~7
It, is made bacterial strain after activation；
Y2. strain inoculated is into fluid nutrient medium after taking step S1 activation obtained, temperature is 25~30 DEG C, revolution is
Under conditions of 150~220 revs/min, shaking table culture 3~7 days, bacterium solution is obtained.
Preferably, every liter of component of the fluid nutrient medium is as follows: soybean, dregs of beans 100g being taken to wear into soya-bean milk filtering, waste；It is white
Granulated sugar, sucrose or brown sugar 100g；Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
15~25g of agar, water are settled to 1 liter, and pH is 7.4~7.6.
Compared with prior art, the invention has the following advantages:
Liquid fertilizer nutrition prepared by the present invention is balanced, hormone-free, and complete water-soluble, amino nitrogen is easy to absorb, and does not hurt plant
Strain, use are safe.Growth deformity, low quality, the resistance difference etc. that crop generates due to a lack of microelement can be effectively relieved
Phenomenon enhances disease resistance, improves crop quality.
The fruit quality that liquid fertilizer of the invention can effectively facilitate fruit corn improves, and improves fruit mouthfeel, and have
Conducive to the growth and development of corn, the generation that fruit corn inane fruit can be effectively prevent, suppress fruit.
It applies protein content in the fruit corn of liquid fertilizer of the invention and increases 78.0% compared to the control group, compare
Ratio group has been higher by 20.7%；Amino acid content increases 1 times compared to the control group, increases 36.8% compared to comparative example group, in fact
Existing per mu yield increases that the nearly three times liquid fertilizers is significant to the improvement effect of the fruit quality of fruit corn, and soluble solid contains
Amount increases 44.8%.However, the liquid fertilizer, which increases the fruit per mu yield and fruit quality of black waxy corn, only has lesser work
With.As it can be seen that liquid fertilizer of the invention has very significant effect to the raising fruit quality of fruit corn, to different cultivars
Corn improve fruit quality fruit selectivity with higher.
Streptomyces bacterial strain " TY-001# " provided by the invention, the bacterial strain can adapt to aerobic fementation under hyperosmosis and train
It supports.
Specific embodiment
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of
It limits.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method and apparatus.
Unless stated otherwise, following embodiment agents useful for same and material are commercially available.
The separation and identification of 1 streptomyces bacterial strain of embodiment (Streptomyces)
1. experimental material and condition
(1) in the collected pedotheque in Hunan Province Chenzhou City somewhere.
(2) every liter of component of fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, water are settled to 1
It rises, pH 7.5.
Every liter of component of solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, 15~25g of agar,
Water is settled to 1 liter, pH 7.5.
Culture medium packing, high-pressure sterilizing pot sterilize (121 DEG C, 30min).
(3) aseptic technique: all vessel and apparatus must sterilize (121 DEG C, 30min) through high-pressure sterilizing pot, inoculation
Equal operations carry out in superclean bench.
(4) strain culturing condition: being placed in culture in 25 ± 1 DEG C of illumination insulating boxs (14L:10D), after bacterium colony is formed, turns
The inclined-plane PDA is moved on to, then is transferred in 5 DEG C of refrigerators and saves.
2. strain isolation and screening
(1) separation obtains a streptomycete category bacterial strain T0 from the pedotheque in Hunan Province Chenzhou City somewhere.
(2) by inclined-plane squamous subculture in isolated streptomyces bacterial strain T0 access solid medium, then 5 DEG C of ice are transferred to
It is saved in case.
(3) by the bacterial strain T0 of squamous subculture in fluid nutrient medium, on oscillation shaking table under the conditions of 220 revs/min, 25 DEG C
Culture 5 days.
(4) the T0 liquid bacteria after cultivating 5 days, is placed at 20 watts of ultraviolet radiator 25cm and irradiates 2 hours.
(5) it will be cultivated 5 days in the T0 liquid bacteria access solid slope culture medium after irradiation 25 DEG C, then choose and grow most
Good surviving colonies access solid medium squamous subculture.
(6) several processes all over step (2)~(5) are repeated to recycle.Each in repetitive process circulation, solid medium
All increase by 1 gram with the sodium chloride dosage in fluid nutrient medium, until the sodium chloride dosage in solid medium and fluid nutrient medium
Reach 25 grams.The healthy and strong bacterial strain finally survived is subjected to squamous subculture, then is transferred in 5 DEG C of refrigerators and saves.
(7) the osmophilic strain bacterial strain T0 that will be filtered out above carries out fermentation production run.Through 7 days 25 DEG C open wide it is aerobic
After fermentation, amino nitrogen in fermentation liquid (measuring ammonia nitrogen standard detection by formol titration) is measured, amino nitrogen is picked out and contains
(>=0.7mg/L) highest bacterial strain is measured as final production bacterial strain, is named " TY-001# ".
Culture medium used in fermentation production run is as follows: taking soybean 100g to wear into soya-bean milk filtering, abandons bean dregs；White granulated sugar
100g is settled to 1000mL, pH 7.5.
3. the identification of bacterial strain " TY-001# "
(1) Morphological Identification
Morphological feature are as follows: bacterium colony is rounded, milky, the smooth of the edge, shows there is condensed milk shape gloss；Spore Gram's staining
It is positive.
Morphological Identification shows that the bacterial strain belongs to streptomyces.
In conclusion bacterial strain " TY-001# " is streptomyces, Guangdong Province's microbial bacteria is preserved on March 24th, 2017
Kind collection, deposit number are GDMCC No.:60152, and preservation address is that the province of GuangZhou, China city martyr Road 100 is micro-
Biological five building, institute's laboratory building.
A kind of preparation for the liquid fertilizer for improving fruit corn fruit quality of embodiment 2
It is a kind of improve fruit corn fruit quality liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. 0.1 part of chitosan oligosaccharide, amino acid the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
162 parts, 2 parts of potassium sulfate, 52 parts of calcium superphosphate, 5 parts of boric acid, 1 part of calcium gluconate, 3 parts of white vitriol obtain the liquid
Fertilizer.
A kind of preparation for the liquid fertilizer for improving fruit corn fruit quality of embodiment 3
It is a kind of improve fruit corn fruit quality liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. the configuration of liquid fertilizer: addition addition 0.5 part of the chitosan oligosaccharide, ammonia into 1000 parts of active liquid fertilizer substrate preparations
Base acid 170 parts, 3 parts of potassium sulfate, 56 parts of calcium superphosphate, 8 parts of boric acid, 3 parts of calcium gluconate, 6 parts of white vitriol obtain liquid
Fertilizer；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=0.4mg/g, content of organic matter 119g/L.
A kind of preparation for the liquid fertilizer for improving fruit corn fruit quality of embodiment 4
It is a kind of improve fruit corn fruit quality liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. the configuration of liquid fertilizer: addition addition 1 part of the chitosan oligosaccharide, amino into 1000 parts of active liquid fertilizer substrate preparations
Acid 175 parts, 5 parts of potassium sulfate, 60 parts of calcium superphosphate, 10 parts of boric acid, 3 parts of calcium gluconate, 7 parts of white vitriol obtain Liquid Fertilizer
Material；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=0.4mg/g, content of organic matter 123g/L.Its
Middle phosphate fertilizer is ammonium dihydrogen phosphate, and Ca, B, Mg, Zn, Fe, Mn are respectively derived from calcium gluconate, boric acid, magnesium sulfate, chelated zinc, chela
Close iron, Chelated Manganese.
Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017,
Deposit number is GDMCC No.:60152.
A kind of preparation for the liquid fertilizer for improving fruit corn fruit quality of embodiment 5
It is a kind of improve fruit corn fruit quality liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. the configuration of liquid fertilizer: addition addition 3 parts of the chitosan oligosaccharide, amino into 1000 parts of active liquid fertilizer substrate preparations
Acid 178 parts, 10 parts of potassium sulfate, 68 parts of calcium superphosphate, 18 parts of boric acid, 8 parts of calcium gluconate, 12 parts of white vitriol obtain liquid
Fertilizer；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=0.4mg/g, content of organic matter 162g/L.
A kind of preparation for the liquid fertilizer for improving fruit corn fruit quality of comparative example 1
It is a kind of improve fruit corn fruit quality liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S3. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S4. the configuration of liquid fertilizer: addition addition 1 part of the chitosan oligosaccharide, poly- day into 1000 parts of active liquid fertilizer substrate preparations
Winter 175 parts of propylhomoserin of door, 30 parts of potassium sulfate, 60 parts of calcium superphosphate, 10 parts of boric acid, 3 parts of calcium gluconate, 7 parts of white vitriol
To liquid fertilizer；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=0.4mg/g, the content of organic matter are
122g/L。
Improve the application effect appraisal of the liquid fertilizer of fruit corn fruit quality:
Nutrient needed for liquid fertilizer can quickly supplement plant strain growth promotes fruit corn growth, improves fruit corn
Mouthfeel and fruit quality.To verify the liquid fertilizer prepared by the present invention practical effect that large-scale demonstration is applied on fruit corn
Fruit, applicant's selection have carried out experiment and demonstration on fruit corn.
1 materials and methods
1.1 for examination place 1 be located in Zixing City fruit corn base, 12 mu of testing ground area, soil fertility level compared with
Uniformly.
It is located in Jianning city black waxy corn base for examination place 2,12 mu of testing ground area, soil fertility level is more equal
It is even.
1.2 for studying 1 fruit corn of object, plant line-spacing 0.8m*0.4m, the growing way golden mean of the Confucian school and consistent.
For studying 2 black waxy corn of object, plant line-spacing 0.8m*0.4m, the growing way golden mean of the Confucian school and consistent.
1.3 liquid fertilizer for trying to improve fruit corn fruit quality made from fertilizer embodiment 2~5, product forms are
Liquid.
1.4 it is each for examination place carry out 3 test process:
1. experimental group: every month, which pours, after plantation applies once for trying fertilizer, 500mL/ mus each, dilutes 200 times.
2. control group (CK): per acre every time with the progress of same experimental group equivalent clear water same time, other measures of fertilizer are the same as processing
①。
3. comparative example group: being carried out every time with the chemical fertilizer same time prepared by the comparative example 1 of same experimental group equivalent per acre, other are applied
The same processing of fertile measure is 1..
Experimental group and control group, comparative example group are managed by local conventional cultivation technology.Each processing is tested with per acre
It for cell, is repeated 3 times, management is consistent.Each group randomly selects 10 plants of fruit corns, and statistical average fruit corn flowers and fruits quantity is put down
Mean value measures fruit situation.
2 results and analysis
The influence of 2.1 pairs of fruits nutrition substances
As shown in Table 1, it applies protein content in the fruit corn of the liquid fertilizer and increases 78.0% compared to the control group,
20.7% has been higher by than comparative example group；Amino acid content increases 1 times compared to the control group, increases compared to comparative example group
36.8%.However, comparing black waxy corn it is found that applying the liquid fertilizer for the rush of the absorption of nutrient ingredients of black waxy corn
It is smaller into acting on.
1 fruit transverse diameter measurement result of table
The influence of 2.2 pairs of per mu yields
Statistics strain number per acre, random 10 points of samplings, count fruiting number, claim fresh fruit weight, calculates fruiting number, single fresh fruit weight
Average value calculates theoretical per mu yield, as shown in table 2.
Through calculating, 2000 plants of corns are planted per acre, extract 10 plants of corn fruiting numbers, single fresh fruit counterpoise, fruit ear number and mu
The average value of yield such as table 2.As shown in Table 2, the single fresh fruit weight of the fruit corn of the liquid fertilizer has been used to increase compared to the control group
Add 46.4%, has realized that per mu yield increases nearly three times, effect is considerable.However, fruit retention of the liquid fertilizer to black waxy corn
Rate and per mu yield increase only more small effect.As it can be seen that liquid fertilizer of the invention has the flower and fruit protecting of fruit corn
Very significant effect, to the corn of different cultivars selectivity with higher.
The result situation and per mu yield of the different corn quality different disposals of table 2
| Processing | Fruit ear fruiting number/ | Fresh fruit counterpoise/kg | Per mu yield/kg |
| Fruit corn experimental group | 16 | 0.41 | 1312 |
| Fruit corn control group | 6 | 0.28 | 336 |
| Fruit corn comparative example group | 10 | 0.37 | 740 |
| Black waxy corn experimental group | 10 | 0.39 | 780 |
| Black waxy corn control group | 7 | 0.33 | 462 |
| Black waxy corn comparative example group | 10 | 0.35 | 700 |
The influence of 2.3 pairs of soluble solid contents
It takes several fresh fruit fruits to squeeze the juice at random, measures soluble fixture content, experimental group and control group respectively measure 5
It is secondary, it is averaged.Experimental result is as shown in table 3.
As shown in Table 3, which improves a lot to the fruit quality of corn, and soluble solid content is opposite
44.8% is increased in control group, increases 20.6% compared to comparative example group.However, the fruit quality to black waxy corn promotes effect
Fruit is not obvious.
Table 3
| Processing | Soluble solid content/% |
| Fruit corn experimental group | 15.2 |
| Fruit corn control group | 10.5 |
| Fruit corn comparative example group | 12.6 |
| Black waxy corn experimental group | 11.9 |
| Black waxy corn control group | 10.2 |
| Black waxy corn comparative example group | 11.1 |
The above description is only a preferred embodiment of the present invention, is not intended to restrict the invention, for those skilled in the art
For member, the invention may be variously modified and varied.All within the spirits and principles of the present invention, it is made it is any modification,
Equivalent replacement, improvement etc. should be included within scope of the invention.
Claims (8)
1. a kind of liquid fertilizer for improving fruit corn fruit quality, which is characterized in that the liquid fertilizer is by following substance group
At: 1000 parts of active liquid fertilizer substrate preparation, 62 ~ 191 parts of amino acid 1,2 ~ 10 parts of potassium sulfate, crosses phosphorus at 0.1 ~ 3 part of chitosan oligosaccharide
52 ~ 68 parts of sour calcium, 5 ~ 18 parts of boric acid, 1 ~ 8 part of calcium gluconate, 3 ~ 12 parts of white vitriol；Total nitrogen contains in the liquid fertilizer
Amount >=2.5mg/g, amino nitrogen content >=0.4mg/g, the content of organic matter are 110 ~ 165g/L；The active liquid fertilizer substrate preparation
It is obtained by streptomyces bacterial strain " TY-001# " fermentation；The culture medium of streptomyces bacterial strain " TY-001# " are as follows: soybean is taken to be soaked
After steeping or dregs of beans being taken to carry out pre fermentation, it is beaten, is filtered；Then after sugar being added into filtrate, water is added to be settled in culture medium
Concentration >=100g/L of sugar, pH are 7.4 ~ 7.6；
The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point
Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city
Martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
2. improving the liquid fertilizer of fruit corn fruit quality according to claim 1, which is characterized in that the liquid fertilizer
By following material composition: 1000 parts of active liquid fertilizer substrate preparation, 0.5 ~ 1.5 part of chitosan oligosaccharide, 70 ~ 178 parts of amino acid 1, sulfuric acid
3 ~ 8 parts of potassium, 56 ~ 62 parts of calcium superphosphate, 8 ~ 12 parts of boric acid, 3 ~ 5 parts of calcium gluconate, 6 ~ 9 parts of white vitriol；The Liquid Fertilizer
Total nitrogen content >=2.5mg/g in material, amino nitrogen content >=0.4mg/g, the content of organic matter are 115 ~ 130g/L.
3. improving the liquid fertilizer of fruit corn fruit quality according to claim 1, which is characterized in that the liquid fertilizer
By following material composition: 1000 parts of active liquid fertilizer substrate preparation, 75 parts of amino acid 1,5 parts of potassium sulfate, crosses phosphorus at 1 part of chitosan oligosaccharide
60 parts of sour calcium, 10 parts of boric acid, 3 parts of calcium gluconate, 7 parts of white vitriol；Total nitrogen content >=2.5mg/ in the liquid fertilizer
G, amino nitrogen content >=0.4mg/g, the content of organic matter are 119 ~ 126g/L.
4. improving the liquid fertilizer of fruit corn fruit quality according to claim 1, which is characterized in that the liquid fertilizer
The weight ratio of middle N, P, K are 25 ~ 32:12 ~ 18:1, preferably 28:16:1.
5. improving the liquid fertilizer of fruit corn fruit quality according to claim 1, which is characterized in that the amino acid is
The mixed amino acid solution that poly- asparagus fern door propylhomoserin, lysine, tryptophan are prepared in 1:1:1 ratio.
6. improving the liquid fertilizer of fruit corn fruit quality according to claim 1, which is characterized in that the boric acid 5 ~ 18
3 ~ 12 parts of part, 1 ~ 8 part of calcium gluconate, white vitriol each component ratios are 2 ~ 5:2:3 ~ 6.
7. a kind of preparation method for the liquid fertilizer for improving fruit corn fruit quality, which comprises the following steps:
S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then toward filter
After sugar is added in liquid, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4 ~ 7.6；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, at 20 ~ 28 DEG C after continuing fermentation 7 ~ 8 days, obtain
To fermentation liquid；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；
S5. the configuration of liquid fertilizer: toward active liquid fertilizer substrate preparation addition chitosan oligosaccharide, amino acid, potassium sulfate, phosphorus is crossed
Sour calcium, boric acid, calcium gluconate, white vitriol, selenite obtain the liquid fertilizer.
8. improving the preparation method of the liquid fertilizer of fruit corn fruit quality according to claim 7, which is characterized in that institute
State its amino-acid nitrogen content >=4.5g/L after active liquid fertilizer substrate preparation is stored 15 days.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329754A (en) * | 2011-09-29 | 2012-01-25 | 北京可力美施生物科技有限公司 | Novel strain of streptomyces fradiae and application thereof |
| CN104886089A (en) * | 2015-06-23 | 2015-09-09 | 郭红甫 | Application of active polysaccharides in corn top rot control |
| CN106431629A (en) * | 2016-08-31 | 2017-02-22 | 广德明凯蔬果种植家庭农场 | Adjusting foliar fertilizer |
| CN106676040A (en) * | 2016-12-16 | 2017-05-17 | 吉林农业大学 | Streptomyces griseoplanus, application thereof and microbial agent |
-
2017
- 2017-12-25 CN CN201711427364.8A patent/CN109956813A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329754A (en) * | 2011-09-29 | 2012-01-25 | 北京可力美施生物科技有限公司 | Novel strain of streptomyces fradiae and application thereof |
| CN104886089A (en) * | 2015-06-23 | 2015-09-09 | 郭红甫 | Application of active polysaccharides in corn top rot control |
| CN106431629A (en) * | 2016-08-31 | 2017-02-22 | 广德明凯蔬果种植家庭农场 | Adjusting foliar fertilizer |
| CN106676040A (en) * | 2016-12-16 | 2017-05-17 | 吉林农业大学 | Streptomyces griseoplanus, application thereof and microbial agent |
Non-Patent Citations (1)
| Title |
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| 李颖等: "《现代微生物研究技术》", 31 October 2008, 中国农业大学出版社 * |
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