CN102160642A - Method for preparing Cordyceps rice food - Google Patents

Method for preparing Cordyceps rice food Download PDF

Info

Publication number
CN102160642A
CN102160642A CN2011100644594A CN201110064459A CN102160642A CN 102160642 A CN102160642 A CN 102160642A CN 2011100644594 A CN2011100644594 A CN 2011100644594A CN 201110064459 A CN201110064459 A CN 201110064459A CN 102160642 A CN102160642 A CN 102160642A
Authority
CN
China
Prior art keywords
northern
caterpillar fungus
chinese caterpillar
temperature
cultivated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN2011100644594A
Other languages
Chinese (zh)
Other versions
CN102160642B (en
Inventor
梁轶聪
李佃场
王漪
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nantong Seven Tourism Development Co Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN2011100644594A priority Critical patent/CN102160642B/en
Publication of CN102160642A publication Critical patent/CN102160642A/en
Application granted granted Critical
Publication of CN102160642B publication Critical patent/CN102160642B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • Y02P60/216

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a method for preparing Cordyceps rice foods. The method has the advantages of short production cycle, stable quality of products and no pollution, and ensures that the requirements for food safety can be met. The method comprises the following specific steps: inoculating a Cordyceps liquid seed suspension which is obtained by virtue of liquid culture to rice which is swollen by water and sterilized; performing solid fermentation in a dark room and a light room alternately to produce Cordyceps-fermented rice; and further processing to produce the Cordyceps rice foods.

Description

A kind of preparation method of northern Chinese caterpillar fungus paddy food
Technical field
The present invention relates to a kind of method that adopts solid fermentation method to produce northern Chinese caterpillar fungus paddy food.
Background technology
Northern Chinese caterpillar Fungus (being called for short northern Chinese caterpillar fungus) is also named Cordyceps militaris, belongs to the Clavicipitaceae Cordyceps, belongs to xenogenesis together with Cordyceps sinensis, is a kind of medicinal fungi of preciousness.Chinese caterpillar fungus is lower for the requirement of growing environment in north, liquid fermentation can form mycelium, artificial extensive solid culture can obtain fructification, medical value similar (Cordyceps sinensis polysaccharide of northern Chinese caterpillar fungus and cordycepic acid to the Cordyceps sinensis that has won fame both at home and abroad, suitable with the content of natural cordyceps, content of cordycepin is 35 times of natural cordyceps).Along with development of science and technology, people have reached brand-new stage to the development and use of northern Chinese caterpillar fungus, multiple northern Cordyceps occurred, and still, northern Chinese caterpillar Fungus should not arbitrarily add in food as medicinal material.
And along with growth in the living standard, people are more and more higher to the requirement of the nutrition of food and function, the paddy that occurs composition such as many interpolations health care medicinal materials thus, the patent 200410015616.7 of University Of Science and Technology Of Tianjin provides a kind of solid state fermentation to produce the method for ferment rice bran, edible bacteriums such as glossy ganoderma are inoculated in the rice bran, make foods such as rice bran soymilk powder, thereby improve the nutritional labeling of rice bran; Patent 98103039.4 " preparation method of Chinese caterpillar fungus health food " also is that liquid spawn is inserted in rice or the wheat broth, but foregoing invention is not carried out strictness control to cultural hypha and seeded process, is difficult to satisfy the great attention of current society for food security.
Summary of the invention
The solid fermentation preparation method that the purpose of this invention is to provide a kind of northern Chinese caterpillar fungus paddy, the requirement of food security is satisfied in short production cycle, the constant product quality of this method, no any pollution.
Concrete technical scheme is as follows: will be inoculated in the paddy through soaking, sterilizing through the northern Chinese caterpillar fungus liquid seeds liquid of Liquid Culture, the solid fermentation method that adopts dark, bright chamber to replace prepares northern Chinese caterpillar fungus fermentation paddy.
1, northern cordyceps species inclined-plane cultivation
Northern cordyceps species is inoculated on slant medium, under 24~27 ℃ temperature, cultivated the consisting of of its slant medium 3~6 days: dehydrated potato powder 13~18%, corn flour 0.5~9%, wheat bran 0~10%, glucose 1~2.5%, peptone 0.2~0.5%, yeast extract 0.3~0.8%, magnesium sulfate 0.1~0.3%, Cobastab 10~0.02%, agar 2.5~3%, skimmed milk power 1~2%, the pH value of control culture medium is 5~7.5, preferred 5~7, ideal situation is 6.5.
Discover that glucose is as the important component of growth, consumption should not surpass 2.5%, and the too high meeting of concentration of glucose causes the osmotic pressure of solution too high, and the moisture in the bacterial classification precipitate in the solution, causes bacterium cell death; The present invention adopts dehydrated potato powder, corn flour, wheat bran and glucose that carbon source is provided, and milk powder and yeast extract replenish nitrogenous source, and the optimum content of carbon source, nitrogenous source is a dehydrated potato powder 14~16%, corn flour 5~8%, wheat bran 3~8%, glucose 2~2.5%, yeast extract 0.5~0.8%; Peptone is rich in amino acid, adds the energy for growth that peptone improves bacterial classification, and its content is preferably 0.4~0.5%.In addition, in culture medium, add Cobastab 1Can make the sturdy of mycelial growth, because Cobastab in the wheat bran 1Content is higher, as not adding wheat bran, can select to add Cobastab 10~0.02%; Vitamin E promotes growing of mycelia, also can add 0.02~0.04% vitamin E promotion bacterial classification growth in ideal conditions.
The growth of bacterial classification also needs mineral matter element, for example iron, magnesium, phosphorus, potassium, sodium, copper etc.The present invention adds the elements such as phosphorus, iron and other vitamin that the skimmed milk power that is rich in a large amount of trace elements provides growth to need, and its addition is 1~2%, is preferably 1~1.5%.
Temperature is for the bigger factor of the growth effect of bacterial classification, and generally speaking, temperature is high more, and mycelial growth is fast more, but growth should not store polysaccharide soon, does not advise that temperature is higher than 27 ℃, and under the lower situation of temperature, bacterial classification can stop growing.Therefore, suitable temperature is 24~27 ℃, and preferred optimum growth temp is 26.5~27 ℃.
2, the cultivation of northern Chinese caterpillar fungus liquid seeds liquid
Get a certain amount of slant medium and cultivate the northern cordyceps species of activation, receive fluid nutrient medium, under 24~27 ℃ temperature, cultivated 5~10 days, and made northern Chinese caterpillar fungus liquid spawn, the consisting of of fluid nutrient medium: potato 18~22%, glucose 1~2.5%, skimmed milk power 0.1~0.5%, potassium dihydrogen phosphate 0.01~0.06%, magnesium sulfate 0~0.3%.
3, paddy and water were soaked in fermentation tank 2 hours according to the ratio of 2:1~6:1, then at 115~130 ℃ of temperature, 1.1Kg/cm 2Under the pressure, sterilized 15~30 minutes, wait to inoculate after being cooled to normal temperature.
4, with cultured northern Chinese caterpillar fungus liquid seeds liquid, receive on the sterilization paddy by 10~22% inoculum concentration, carry out dark, the bright solid fermentation that replaces.Lucifuge was cultivated 5~10 days under 24~27 ℃ of conditions, cultivated under illumination condition 10~20 days then, at room temperature carried out drying with natural wind at last, reached about 8-10% up to moisture.
5, further be processed into paddy food.
Through the northern Chinese caterpillar fungus liquid seeds liquid of inclined-plane cultivation and Liquid Culture, preferably receive in the sterilization paddy by 15~20% inoculum concentration, alternately cultivate in darkroom and bright chamber, carry out solid fermentation.After the fermentation ends, carry out air dry, its moisture is reached about 9%.
Bacterial classification production of the present invention is carried out in strict accordance with the sterile working rules, the quality of culture medium raw material and additive detects through strict, the cereal that uses is through high-temperature sterilization, and sweat is got rid of assorted bacterium and noxious pollutant disturbs, thereby makes northern Chinese caterpillar fungus fermentation cereal possess good edible safety.
The specific embodiment
Embodiment 1
Preparing northern Chinese caterpillar fungus rice step by solid fermentation method describes.
1, northern cordyceps species inclined-plane cultivation
Northern cordyceps species is inoculated on slant medium, under 26.5~27 ℃ temperature, cultivated 3~6 days, the consisting of of its slant medium: dehydrated potato powder 15%, corn flour 6%, wheat bran 3%, glucose 2.5%, peptone 0.3%, yeast extract 0.8%, magnesium sulfate 0.1%, Cobastab 10.02%, agar 2%, skimmed milk power 2%, the pH value of control culture medium is 6.5.
2, the cultivation of northern Chinese caterpillar fungus liquid seeds liquid
Get a certain amount of slant medium and cultivate the northern cordyceps species of activation, receive fluid nutrient medium, under 24~27 ℃ temperature, cultivated 7 days, and made northern Chinese caterpillar fungus liquid spawn, the consisting of of fluid nutrient medium: potato 22%, glucose 2.5%, skimmed milk power 0.5%, potassium dihydrogen phosphate 0.01%, magnesium sulfate 0.3%.
3, rice and water were soaked in fermentation tank 2 hours according to the ratio of 5:1, then at 121 ℃ of temperature, 1.1Kg/cm 2Under the pressure, sterilized 30 minutes, wait to inoculate after being cooled to behind the normal temperature.
4, with cultured northern Chinese caterpillar fungus liquid seeds liquid, receive on the sterilization paddy by 15% inoculum concentration, carry out dark, the bright solid fermentation that replaces.Lucifuge was cultivated 10 days under 24~27 ℃ of conditions, cultivated 15 days under illumination condition then, at room temperature carried out drying with natural wind at last, made its moisture reach 10%.
Embodiment 2
Preparing northern Chinese caterpillar fungus rice bran step by solid fermentation method describes.
1, northern cordyceps species inclined-plane cultivation
Northern cordyceps species is inoculated on slant medium, under 26.5~27 ℃ temperature, cultivated 5 days, consisting of of its slant medium: dehydrated potato powder 18%, corn flour 0.5%, wheat bran 10%, glucose 2.5%, peptone 0.4%, yeast extract 0.6%, magnesium sulfate 0.3%, vitamin E 0.02%, agar 3%, skimmed milk power 2%, the pH value of control culture medium is 6.
2, the cultivation of northern Chinese caterpillar fungus liquid seeds liquid
Get a certain amount of slant medium and cultivate the northern cordyceps species of activation, receive fluid nutrient medium, under 25~26.5 ℃ temperature, cultivated 7 days, and made northern Chinese caterpillar fungus liquid spawn, the consisting of of fluid nutrient medium: potato 22%, glucose 2.5%, skimmed milk power 0.5%, potassium dihydrogen phosphate 0.01%, magnesium sulfate 0.3%.
3, rice bran and water were soaked in fermentation tank 2 hours according to the ratio of 6:1, then at 115 ℃ of temperature, 1.1Kg/cm 2Under the pressure, sterilized 30 minutes, wait to inoculate after being cooled to behind the normal temperature.
4, with cultured northern Chinese caterpillar fungus liquid seeds liquid, receive on the sterilization paddy by 22% inoculum concentration, carry out dark, the bright solid fermentation that replaces.Lucifuge was cultivated 10 days under 25~26.5 ℃ of conditions, cultivated 15 days under illumination condition then, at room temperature carried out drying with natural wind at last, made its moisture reach 10%.
5, the further processing of northern Chinese caterpillar fungus rice bran is added in the cereal preparation.

Claims (1)

1. the preparation method of a northern Chinese caterpillar fungus paddy food, it adopts the solid fermentation method preparation, may further comprise the steps:
1), northern cordyceps species inclined-plane cultivation
Northern cordyceps species is inoculated on slant medium, under 24~27 ℃ temperature, cultivated the consisting of of its slant medium 3~6 days: dehydrated potato powder 13~18%, corn flour 0.5~9%, wheat bran 0~10%, glucose 1~2.5%, peptone 0.2~0.5%, yeast extract 0.3~0.8%, magnesium sulfate 0.1~0.3%, Cobastab 10~0.02%, agar 1~3%, skimmed milk power 1~2%, the pH value of control culture medium is 5~7.5, preferred 5~7;
2), the cultivation of northern Chinese caterpillar fungus liquid seeds liquid
Get a certain amount of slant medium and cultivate the northern cordyceps species of activation, receive fluid nutrient medium, under 24~27 ℃ temperature, cultivated 5~10 days, and made northern Chinese caterpillar fungus liquid spawn, the consisting of of fluid nutrient medium: potato 18~22%, glucose 1~2.5%, skimmed milk power 0.1~0.5%, potassium dihydrogen phosphate 0.01~0.06%, magnesium sulfate 0~0.3%;
3), paddy and water were soaked in fermentation tank 2 hours according to the ratio of 2:1~6:1, then at 115~130 ℃ of temperature, 1.1Kg/cm 2Under the pressure, sterilized 15~30 minutes, wait to inoculate after being cooled to normal temperature;
4), with cultured northern Chinese caterpillar fungus liquid seeds liquid, receive on the sterilization paddy by 10~22% inoculum concentration, carry out dark, the bright solid fermentation that replaces: lucifuge was cultivated 5~10 days under 24~27 ℃ of conditions, under illumination condition, cultivated 10~20 days then, at room temperature carry out drying at last, reach about 8-10% up to moisture with natural wind.
CN2011100644594A 2011-03-17 2011-03-17 Method for preparing Cordyceps rice food Expired - Fee Related CN102160642B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2011100644594A CN102160642B (en) 2011-03-17 2011-03-17 Method for preparing Cordyceps rice food

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2011100644594A CN102160642B (en) 2011-03-17 2011-03-17 Method for preparing Cordyceps rice food

Publications (2)

Publication Number Publication Date
CN102160642A true CN102160642A (en) 2011-08-24
CN102160642B CN102160642B (en) 2012-07-04

Family

ID=44462310

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2011100644594A Expired - Fee Related CN102160642B (en) 2011-03-17 2011-03-17 Method for preparing Cordyceps rice food

Country Status (1)

Country Link
CN (1) CN102160642B (en)

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102388795A (en) * 2011-11-08 2012-03-28 付湘晋 Method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds
CN102612984A (en) * 2012-03-10 2012-08-01 何寒 Method for preparing cordyceps sinensis mycelia with fresh and tender sweet corn
CN102613368A (en) * 2012-04-24 2012-08-01 福建农大科技开发总公司 North cordyceps sinensis herba epimedii compound tea bag product and producing method thereof
CN102823806A (en) * 2012-09-20 2012-12-19 王永显 Method for producing fermented oatmeal using Cordyceps militaris
CN102940185A (en) * 2012-11-08 2013-02-27 广东省微生物研究所 Edible mushroom flavor nutrient rice milk and preparation method of edible mushroom flavor nutrient rice milk
CN103271281A (en) * 2013-06-09 2013-09-04 食品行业生产力促进中心 Method for producing cordyceps militaris fermentation coarse cereals through solid-state fermentation
CN103652627A (en) * 2013-12-26 2014-03-26 大连盖世生物技术有限公司 Cordyceps rice-flour noodle and processing method thereof
CN103960357A (en) * 2013-02-06 2014-08-06 殷锋科 Ecological transformation process for producing low-glycemic-index staple food by utilizing cordyceps militaris
CN104686202A (en) * 2015-03-20 2015-06-10 江南大学 Cordyceps sinensis sweet potatoes
CN104718987A (en) * 2015-03-20 2015-06-24 江南大学 Cordyceps sinensis chenopodium quinoa
CN104718989A (en) * 2015-03-20 2015-06-24 江南大学 Cordyceps sinensis dasheen
CN104824728A (en) * 2015-03-20 2015-08-12 江南大学 Cordyceps sinensis sesames
CN104823691A (en) * 2015-03-20 2015-08-12 江南大学 Cordyceps corn
CN104824586A (en) * 2015-03-20 2015-08-12 江南大学 Cordyceps sinensis cassava
CN104824574A (en) * 2015-03-20 2015-08-12 江南大学 Cordyceps sinensis broad bean
CN104824588A (en) * 2015-03-20 2015-08-12 江南大学 Cordyceps sinensis potatoes
CN105053807A (en) * 2015-08-18 2015-11-18 雷色香 Chinese caterpillar fungus vermicelli
CN108371282A (en) * 2016-10-12 2018-08-07 浙江泛亚生物医药股份有限公司 A kind of cicada fungus rice and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1208575A (en) * 1998-07-21 1999-02-24 李健 Method for preparing health-care food contg. Chinese caterpillar fungus
CN1650686A (en) * 2005-01-06 2005-08-10 江苏省农业科学院 Solid culturing method of north cateripillar fungus
CN101176521A (en) * 2006-11-06 2008-05-14 张刚 North cordyceps rice flour product and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1208575A (en) * 1998-07-21 1999-02-24 李健 Method for preparing health-care food contg. Chinese caterpillar fungus
CN1650686A (en) * 2005-01-06 2005-08-10 江苏省农业科学院 Solid culturing method of north cateripillar fungus
CN101176521A (en) * 2006-11-06 2008-05-14 张刚 North cordyceps rice flour product and preparation method thereof

Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102388795A (en) * 2011-11-08 2012-03-28 付湘晋 Method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds
CN102612984A (en) * 2012-03-10 2012-08-01 何寒 Method for preparing cordyceps sinensis mycelia with fresh and tender sweet corn
CN102612984B (en) * 2012-03-10 2013-09-04 何寒 Method for preparing cordyceps sinensis mycelia with fresh and tender sweet corn
CN102613368A (en) * 2012-04-24 2012-08-01 福建农大科技开发总公司 North cordyceps sinensis herba epimedii compound tea bag product and producing method thereof
CN102823806A (en) * 2012-09-20 2012-12-19 王永显 Method for producing fermented oatmeal using Cordyceps militaris
CN102823806B (en) * 2012-09-20 2013-09-25 王永显 Method for producing fermented oatmeal using Cordyceps militaris
CN102940185A (en) * 2012-11-08 2013-02-27 广东省微生物研究所 Edible mushroom flavor nutrient rice milk and preparation method of edible mushroom flavor nutrient rice milk
CN103960357A (en) * 2013-02-06 2014-08-06 殷锋科 Ecological transformation process for producing low-glycemic-index staple food by utilizing cordyceps militaris
CN103271281A (en) * 2013-06-09 2013-09-04 食品行业生产力促进中心 Method for producing cordyceps militaris fermentation coarse cereals through solid-state fermentation
CN103652627A (en) * 2013-12-26 2014-03-26 大连盖世生物技术有限公司 Cordyceps rice-flour noodle and processing method thereof
CN104686202A (en) * 2015-03-20 2015-06-10 江南大学 Cordyceps sinensis sweet potatoes
CN104718987A (en) * 2015-03-20 2015-06-24 江南大学 Cordyceps sinensis chenopodium quinoa
CN104718989A (en) * 2015-03-20 2015-06-24 江南大学 Cordyceps sinensis dasheen
CN104824728A (en) * 2015-03-20 2015-08-12 江南大学 Cordyceps sinensis sesames
CN104823691A (en) * 2015-03-20 2015-08-12 江南大学 Cordyceps corn
CN104824586A (en) * 2015-03-20 2015-08-12 江南大学 Cordyceps sinensis cassava
CN104824574A (en) * 2015-03-20 2015-08-12 江南大学 Cordyceps sinensis broad bean
CN104824588A (en) * 2015-03-20 2015-08-12 江南大学 Cordyceps sinensis potatoes
CN105053807A (en) * 2015-08-18 2015-11-18 雷色香 Chinese caterpillar fungus vermicelli
CN108371282A (en) * 2016-10-12 2018-08-07 浙江泛亚生物医药股份有限公司 A kind of cicada fungus rice and preparation method thereof
CN108371282B (en) * 2016-10-12 2021-12-28 浙江泛亚保健食品有限公司 Cordyceps sobolifera rice and preparation method thereof

Also Published As

Publication number Publication date
CN102160642B (en) 2012-07-04

Similar Documents

Publication Publication Date Title
CN102160642B (en) Method for preparing Cordyceps rice food
CN102132881B (en) Method for preparing lucid ganoderma rice food
CN101897275B (en) Selenium-rich cordyceps culturing method
CN101611767B (en) Method for producing microbial fermentation bait for sea cucumbers
CN102351605B (en) Culture method for liquid fermentation of rare edible-medicinal fungus Sparassis crispa and special medium thereof
CN101113415A (en) Method for solid culture of Cordyceps sinensis edible mushroom
CN107043280A (en) A kind of biological compound fertilizer and preparation method thereof
CN105981581B (en) A kind of artificial culture method of cicada fungus
CN104429604A (en) Sparassis crispa liquid strain culture medium and culture method
CN101991045A (en) Cordyceps militaris rice and solid fermentation preparation method thereof
CN102172275B (en) Method for producing agaricus blazei rice
CN110106090A (en) A kind of Neuraspora crassa strain and its application
CN101194917B (en) Grifola frondosa oral liquid rich in gastrodine, organic selenium and method for preparing the same
CN108795819B (en) Compound microorganism culture and application thereof in production of carotenoid
CN102102095B (en) Method for preparing lysozyme by fermenting marine streptomyces
CN105441334B (en) Produce bacterial strain and its application of grifolan
CN100572548C (en) Improve the high temperature revulsion method of lentinan output
CN108179124B (en) Composite culture medium for culturing spirulina platensis
CN103160550A (en) Preparation method of compound polysaccharide consisting of schizophyllum commune exopolysaccharide and oat polysaccharide
CN110713956A (en) Lysine bacillus S12 and application thereof
Yao et al. Relationship between saccharifying capacity and isolation sources for strains of the Rhizopus arrhizus complex
CN103849575A (en) Production method of single-cell protein
CN110004068A (en) A kind of mushroom strain storage medium and method for preserving
CN104255300A (en) Cultivation method of selenium-enriched cord cepsmilitaris
CN104711208B (en) A kind of lactic acid bacteria with high starch capacity of decomposition

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
ASS Succession or assignment of patent right

Owner name: NANTONG WEEK TOURISM DEVELOPMENT CO., LTD.

Free format text: FORMER OWNER: LI DIANCHANG

Effective date: 20130716

C41 Transfer of patent application or patent right or utility model
COR Change of bibliographic data

Free format text: CORRECT: ADDRESS; FROM: 266061 QINGDAO, SHANDONG PROVINCE TO: 226121 NANTONG, JIANGSU PROVINCE

TR01 Transfer of patent right

Effective date of registration: 20130716

Address after: 226121 Jiangsu province Nantong markets three factory town Road No. 79

Patentee after: Nantong seven Tourism Development Co., Ltd.

Address before: 266061 phase 12-2-402, Li Hai garden, Laoshan District, Shandong, Qingdao

Patentee before: Li Dianchang

CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120704

Termination date: 20180317

CF01 Termination of patent right due to non-payment of annual fee