CN1650686A - Solid culturing method of north cateripillar fungus - Google Patents
Solid culturing method of north cateripillar fungus Download PDFInfo
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- CN1650686A CN1650686A CN 200510037624 CN200510037624A CN1650686A CN 1650686 A CN1650686 A CN 1650686A CN 200510037624 CN200510037624 CN 200510037624 CN 200510037624 A CN200510037624 A CN 200510037624A CN 1650686 A CN1650686 A CN 1650686A
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Abstract
A solid culture method for northern cordyceps features that its solid culture medium is prepared from glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, triammonium citrate, VB, long-grained non-glutinous rice, and silkworm powder. Its advantage is high content of cordycepin in its sporophores.
Description
One, technical field
The present invention is the solid culture based formulas of the functional component content of a kind of raising artificial culture northern Chinese caterpillar Fungus (abbreviation Cordyceps militaris), belongs to the tame technical field of northern Chinese caterpillar Fungus.Be exclusively used in the functional component content that improves in the tame cordyceps militaris fruit body, help improving the health-care efficacy and the medical value of artificial north Chinese caterpillar fungus.
Two, technical background
Northern Chinese caterpillar Fungus is as famous food, medicinal fungi, and its main active is cordycepin (3 '-desoxyadenossine), cordycepic acid (D-mannitol) and Cordyceps sinensis polysaccharide etc.Cordycepin has many physiologically active ingredients and medicinal health care function, as antitumor action, antivirus action, antibacterial action, raise immunity effect etc., and is used as the leading indicator of weighing Chinese caterpillar fungus quality quality.Northern Chinese caterpillar Fungus is the main generation bacterium of cordycepin, and because of the prescription difference of its medium, the content of cordycepin is also different.With cordycepin content in the Cordyceps militaris of medium provided by the invention cultivation is several times and even tens times of wild cordyceps, and the content of cordycepic acid and Cordyceps sinensis polysaccharide is also very high simultaneously.Improving the quality of northern Chinese caterpillar Fungus, mainly is exactly the content, particularly content of cordycepin that further improves cordycepin isoreactivity composition.It has been recognized that the important value of northern Chinese caterpillar Fungus in medical health field, positive active research, cultivation and exploitation northern Chinese caterpillar Fungus.
Lack in the tame culture matrix of northern Chinese caterpillar Fungus that generally uses specially at the biological excitation factor that improves northern Chinese caterpillar Fungus functional component cordycepin at present.The artificial cordyceps functional component content of producing is on the low side.The follow-up exploitation and the industrialization of northern Chinese caterpillar Fungus have been limited.Therefore, in the expansion scale with when increasing output, improve the inside and outside quality of northern Chinese caterpillar Fungus, become the problem that we press for solution.
Three, summary of the invention
The technical problem Cordyceps militaris produces cordycepin and requires medium that a peptide class nutrient component can be provided, and present solid culture medium mostly adopts the interpolation dried silkworm chrysalis meal that nutriment is provided.The present invention is by improving the solid culture based formulas of northern Chinese caterpillar Fungus, makes functional component cordycepin in the artificial cordyceps fruit body (3 '-desoxyadenossine) content improve, and reaches to have that generalization is strong, effect is obvious, has no side effect the purpose of no drug residue.
The solid culture method of a kind of northern Chinese caterpillar Fungus of technical scheme is characterized in that:
The 1000ML Chinese caterpillar fungus is cultivated in the nutrient solution and contains: glucose 15-25g, peptone 15-25g, magnesium sulfate 1.5-2.5g, potassium dihydrogen phosphate 0.5-1.5g, citric acid tri-amonia 1.5-2.5g, Cobastab
1200-250mg;
In every culture bottle solid culture medium: long-grained nonglutinous rice 30-40g, silkworm powder 0.5-1.5g.
The solid culture method of above-mentioned a kind of northern Chinese caterpillar Fungus is characterized in that: glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, citric acid tri-amonia, Cobastab
1It is pure to be analysis.Silkworm powder be four age the silkworm worm to pulverize and cross 60 mesh sieves behind low temperature drying below 40 ℃ standby.The nutrient solution collocation method is earlier with glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, citric acid tri-amonia, Cobastab
1, water dissolves in running water in proportion, adds silkworm powder, long-grained nonglutinous rice again, mixes then, autoclaving got final product after 1.0 hours.
Beneficial effect
The present invention compares with existing artificial cordyceps solid culture matrix, has following advantage:
(1) in the present Cordyceps militaris artificial culture matrix mostly with dried silkworm chrysalis meal as the Cordyceps militaris needed protein source of growing.This seminar has carried out Study on Optimization Combination to various biological and inanimate excitation factors, in prescription, added four age silkworm powder, content of cordycepin improves more than 10% than original formulation in the Cordyceps militaris, Cordyceps militaris output improves more than 10%.
(2) the present invention adopts live body silkworm worm in four ages low temperature drying to pulverize in the back adding medium, has avoided the employing dried silkworm chrysalis meal to cause pollution in silkworm chrysalis processing.
(3) culture medium prescription provided by the present invention, cost is low, technology is simple, and the producer is acceptant, has that generalization is strong, effect is obvious, has no side effect the advantage of no drug residue.
Four, embodiment
A kind of solid culture based formulas that improves the functional component content of artificial culture northern Chinese caterpillar Fungus, its constituent content is:
The 1000g Chinese caterpillar fungus contains in cultivating in the nutrient solution: glucose 20g, peptone 20g, magnesium sulfate 2g, potassium dihydrogen phosphate 1g, citric acid tri-amonia 2g, Cobastab
1200mg;
In every culture bottle medium: long-grained nonglutinous rice 35g, silkworm powder 1g.
Its preparation method is:
(1), the nutrient solution compound method: accurate each component in the above-mentioned nutrient solution of weighing, be dissolved in proportion in the water of requirement, standby.
(2), silkworm powder, long-grained nonglutinous rice put into clean high temperature high voltage resistant cultivating container (glass jar), put into long-grained nonglutinous rice 35g, silkworm powder 1g for every bottle.
(3), every bottle of nutrient solution 55～60ML that adding prepares.
(4), select for use the polyacrylic film of 5 high temperature high voltage resistant to seal glass bottle opening.
(5), prepared culture medium is put into high-pressure sterilizing pot, under 121 ℃, the condition of 1.05Mpa, kept 1.0 hours.
(6), culture medium after sterilization matter is put into transfer room, transfer room is carried out inoculating production behind the overall disinfection in superclean bench.
(7) inoculation back Chinese caterpillar fungus culture bottle is put the special-purpose cultivating chamber of Chinese caterpillar fungus into, and, light wet to it by the conventional cultural method of Cordyceps militaris growth different phase, humidity are regulated and control.
Five, a kind of efficacy test that improves artificial culture northern Chinese caterpillar Fungus medium-height grass cellulose content culture medium prescription:
Test method: provide Cordyceps militaris to produce cultivated species CM-4 by academy of agricultural sciences, Jiangsu Province edible mushroom research and development centre.Adopt comparative formula (present existing prescription) to be: glucose 2%, peptone 2%, magnesium sulfate 0.2%, potassium dihydrogen phosphate 0.1%, citric acid tri-amonia 0.2%, Cobastab
1Trace, long-grained nonglutinous rice 93%, dried silkworm chrysalis meal 1g, water 1: 1.5, the preparation method is the same.Every kind of each lab scale of culture matrix is produced 1000 bottles.According to the production technology of artificial cordyceps, medium is through inoculating, send out bacterium, going out grass.Gather and obtain Cordyceps militaris fruiting body and advance the air dry oven low temperature drying to constant weight.Calculate every bottle and get output mean value.
Result of the test: experimental group has for 1000 bottles pollutes 50 bottles.Control group pollutes 120 bottles.
Through the average every bottle of oven dry fruit body weight of experiment with computing group is 2.75g, and every bottle of average yield of control group is that the 2.51g.. experimental group increases 0.24g for average every bottle than control group output, and output improves about 10%.
The mensuration of cordycepin adopts the HPLC method to measure.Random sampling 0.5g.Sample is with 50% ethanol ultrasonic Extraction.Condition determination is to be chromatographic column with AgilentODS (4.6mm * 250mm, 5 μ l), detects wavelength 260nm, flow velocity 1ml/min, and 25 ℃ of conditions of column temperature, acetonitrile-water carries out wash-out.Reference substance is purchased in Nat'l Pharmaceutical ﹠ Biological Products Control Institute.
Measure cordycepin content through the HPLC method.Every group of three of survey is parallel, results averaged.The experimental group cordycepin content is 1.342%, and the control group cordycepin content has improved about 10% for the 0.122%. cordycepin content.
Promote checking
The present invention in academy of agricultural sciences, Jiangsu Province edible mushroom research and development centre Demonstration Application 3 batches of totally 5 ten thousand bottles of Cordyceps militaris.Its Chinese caterpillar fungus yield and quality all improved a lot than originally, and wherein content of cordycepin improves 10%, and the output of its fruit body improves 13%.Meet the efficacy test result.
Cordycepin content culture medium prescription effect is summed up in a kind of raising artificial culture northern Chinese caterpillar Fungus:
Through the result of overtesting and Demonstration Application, prove that prescription of the present invention can improve the content of the major function composition in the artificial cultivation Cordyceps militaris fruiting body, content of cordycepin can improve about 10%.Also can improve simultaneously the output about 10% of its fruit body.
Claims (6)
1. the solid culture method of a northern Chinese caterpillar Fungus is characterized in that:
The 1000ML Chinese caterpillar fungus is cultivated in the nutrient solution and contains: glucose 15-25g, peptone 15-25g, magnesium sulfate 1.5-2.5g, potassium dihydrogen phosphate 0.5-1.5g, citric acid tri-amonia 1.5-2.5g, Cobastab
1200-250mg;
In every culture bottle solid culture medium: long-grained nonglutinous rice 30-40g, silkworm powder 0.5-1.5g.
2. according to the solid culture method of the described a kind of northern Chinese caterpillar Fungus of claim 1, it is characterized in that: glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, citric acid tri-amonia, Cobastab
1It is pure to be analysis.
3. according to the solid culture method of claim 1 or 2 described a kind of northern Chinese caterpillar Fungus, it is characterized in that: silkworm powder be four age the silkworm worm to pulverize and cross 60 mesh sieves behind low temperature drying below 40 ℃ standby.
4. according to the solid culture method of claim 1 or 2 described a kind of northern Chinese caterpillar Fungus, it is characterized in that: the nutrient solution collocation method is earlier with glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, citric acid tri-amonia, Cobastab
1, water dissolves in running water in proportion, adds silkworm powder, long-grained nonglutinous rice again, mixes then, autoclaving got final product after 1.0 hours.
5. according to the solid culture method of the described a kind of northern Chinese caterpillar Fungus of claim 3, it is characterized in that: the nutrient solution collocation method is earlier with glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, citric acid tri-amonia, Cobastab
1, water dissolves in running water in proportion, adds silkworm powder, long-grained nonglutinous rice again, mixes then, autoclaving got final product after 1.0 hours.
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100375784C (en) * | 2006-01-23 | 2008-03-19 | 孟震 | Complex function type northern Chinese caterpillar fungus and its production method |
| CN101314759B (en) * | 2007-05-29 | 2010-07-21 | 徐良洪 | Artificial north Chinese caterpillar fungus culture medium and preparation method thereof |
| CN102160642A (en) * | 2011-03-17 | 2011-08-24 | 李佃场 | Method for preparing Cordyceps rice food |
| CN102342218A (en) * | 2011-05-07 | 2012-02-08 | 华中农业大学 | Method for producing cordyceps sinensis hyphae by virtue of solid-state fermentation |
| CN102342217A (en) * | 2010-07-29 | 2012-02-08 | 于兴军 | Pollution-free cordyceps militaris strain production or fermentation method |
| CN101066090B (en) * | 2007-05-28 | 2012-05-23 | 李治德 | Aweto activated reinforced immunological feed additive and its production process |
| CN102696402A (en) * | 2012-07-04 | 2012-10-03 | 黑龙江大学 | Culturing method of liquorice-based cordyceps militaris |
| CN103299821A (en) * | 2012-03-08 | 2013-09-18 | 黄友鹰 | Original ecological cordyceps sinensis high yielding and breeding method |
| CN103396193A (en) * | 2013-08-08 | 2013-11-20 | 董亚贤 | Cordycepsmilitaris strain liquid culture medium and preparation method therefor |
| CN103563649A (en) * | 2013-10-10 | 2014-02-12 | 山东晨阳菌业有限公司 | Cultivation method of cordyceps militaris |
| CN104031848A (en) * | 2014-06-30 | 2014-09-10 | 安徽吾悦食品有限公司 | Cordyceps militaris culturing media |
| CN104370620A (en) * | 2013-08-15 | 2015-02-25 | 河北嘉真农业科技有限公司 | Cordyceps militaris cultivation culture media for increasing carotenoid content, and culture method |
| CN105152742A (en) * | 2015-07-15 | 2015-12-16 | 十堰神农武当医药科技园有限公司 | Solid culture medium for Cordyceps sinensis and preparation method |
| CN106718031A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris hot weather cultivating technique |
| CN115005013A (en) * | 2022-07-21 | 2022-09-06 | 重庆华绿北草生物科技有限公司 | Cordyceps militaris high-yield cultivation medium and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1094091A (en) * | 1994-02-05 | 1994-10-26 | 李春燕 | The seed selection of cordyceps militaris excellent species and high yield cultivating method |
| CN1445359A (en) * | 2002-03-14 | 2003-10-01 | 李健 | Technique for artificial breeding Chinese caterpillar fungus with out polypide |
-
2005
- 2005-01-06 CN CNB2005100376241A patent/CN100339471C/en not_active Expired - Fee Related
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100375784C (en) * | 2006-01-23 | 2008-03-19 | 孟震 | Complex function type northern Chinese caterpillar fungus and its production method |
| CN101066090B (en) * | 2007-05-28 | 2012-05-23 | 李治德 | Aweto activated reinforced immunological feed additive and its production process |
| CN101314759B (en) * | 2007-05-29 | 2010-07-21 | 徐良洪 | Artificial north Chinese caterpillar fungus culture medium and preparation method thereof |
| CN102342217A (en) * | 2010-07-29 | 2012-02-08 | 于兴军 | Pollution-free cordyceps militaris strain production or fermentation method |
| CN102160642B (en) * | 2011-03-17 | 2012-07-04 | 李佃场 | Method for preparing Cordyceps rice food |
| CN102160642A (en) * | 2011-03-17 | 2011-08-24 | 李佃场 | Method for preparing Cordyceps rice food |
| CN102342218B (en) * | 2011-05-07 | 2013-06-05 | 华中农业大学 | Method for producing cordyceps sinensis hyphae by virtue of solid-state fermentation |
| CN102342218A (en) * | 2011-05-07 | 2012-02-08 | 华中农业大学 | Method for producing cordyceps sinensis hyphae by virtue of solid-state fermentation |
| CN103299821A (en) * | 2012-03-08 | 2013-09-18 | 黄友鹰 | Original ecological cordyceps sinensis high yielding and breeding method |
| CN102696402A (en) * | 2012-07-04 | 2012-10-03 | 黑龙江大学 | Culturing method of liquorice-based cordyceps militaris |
| CN103396193A (en) * | 2013-08-08 | 2013-11-20 | 董亚贤 | Cordycepsmilitaris strain liquid culture medium and preparation method therefor |
| CN104370620A (en) * | 2013-08-15 | 2015-02-25 | 河北嘉真农业科技有限公司 | Cordyceps militaris cultivation culture media for increasing carotenoid content, and culture method |
| CN103563649A (en) * | 2013-10-10 | 2014-02-12 | 山东晨阳菌业有限公司 | Cultivation method of cordyceps militaris |
| CN103563649B (en) * | 2013-10-10 | 2016-07-06 | 山东晨阳菌业有限公司 | A kind of cordyceps militaris cultivation method |
| CN104031848A (en) * | 2014-06-30 | 2014-09-10 | 安徽吾悦食品有限公司 | Cordyceps militaris culturing media |
| CN104031848B (en) * | 2014-06-30 | 2016-08-17 | 安徽吾悦食品有限公司 | A kind of Cordyceps culture medium |
| CN105152742A (en) * | 2015-07-15 | 2015-12-16 | 十堰神农武当医药科技园有限公司 | Solid culture medium for Cordyceps sinensis and preparation method |
| CN106718031A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris hot weather cultivating technique |
| CN115005013A (en) * | 2022-07-21 | 2022-09-06 | 重庆华绿北草生物科技有限公司 | Cordyceps militaris high-yield cultivation medium and preparation method thereof |
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