CN1208575A - Method for preparing health-care food contg. Chinese caterpillar fungus - Google Patents
Method for preparing health-care food contg. Chinese caterpillar fungus Download PDFInfo
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- CN1208575A CN1208575A CN98103039A CN98103039A CN1208575A CN 1208575 A CN1208575 A CN 1208575A CN 98103039 A CN98103039 A CN 98103039A CN 98103039 A CN98103039 A CN 98103039A CN 1208575 A CN1208575 A CN 1208575A
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Abstract
A health-care food is prepared from three kinds of edible fungus of cordyceps series. The three fungi are cultured separately on slant culture media, then inocculated with the cultured strains on a composite culture media to obtain liquid strain, further it is inocculated into rice or wheat culture media to obtain solid mycellium which is oven-dried, ground and mixed with milk powder, lotus seed powder, Job's tears seed powder, seasame, etc. as auxiliary ingredients, and then it is added with seasonings, packaged and sterilized to obtain the product. It features simple in method and suitable for large scale prodn.
Description
That the present invention relates to is the preparation method of Chinese caterpillar fungus health food, belongs to fungus solids and cultivates the mycelial growth technical field of bioengineering.
Along with scientific technological advance, biotechnology enters each production application field thereupon, particularly since the nineties, utilize the food drink with health role of biotechnology preparation to come out one after another, in a feast for the eyes both at home and abroad at present food drink, most kinds are that with food and medicine consangunity, the same merit of food medicine, food medicine in like manner instant medicine one is seen and is the product innovation of foundational development.Wherein nutritional type occupies the majority, and health is less, and especially comprehensive health care's food powdered agent of being made by multiple biological raw material is more rare, and this is the shortcoming of prior art.
The object of the present invention is to provide a kind of preparation method of Chinese caterpillar fungus health food, can prepare health foodstuff series such as Chinese caterpillar fungus milk extract, Chinese caterpillar fungus lotus heart of a lotus seed powder, the fine and soft electuary of Chinese caterpillar fungus with this method.
Design of the present invention is such, select Cordyceps strain for use, the Grifola frondosa bacterial classification, the mycelium that Agaricus blazei is cultivated is a main raw material, adopt the healthy cell breeding method, according to genetic principle healthy cell is imported in the circulation breeding process, pick bad stay excellent, again pure strong mycelium is separated into little mycelia piece with biotechnology, insert in the semisynthetic medium respectively, cultivate into slant strains, again slant strains is cultivated into liquid spawn in same complex medium, in the ripe rice or wheat broth of the malicious boiling that then its access disappeared, cultivate into the solid mycelium, with the oven dry of grain grain solid mycelium process, pulverize, be mixed, seasoning, packing, sterilization gets product.
Particularly, the preparation method of Chinese caterpillar fungus health food of the present invention comprises the steps:
A, select for use the mycelia piece of worm summer in winter bacterial classification, Grifola frondosa bacterial classification, Agaricus blazei to insert in the semisynthetic medium respectively, cultivate into slant strains;
B, get above-mentioned three kinds of slant strains and insert in the same complex medium, cultivate into liquid spawn;
C, get liquid spawn and insert again in the malicious boiling that the disappeared ripe rice or wheat broth, cultivate into the solid mycelium;
D, with grain grain solid mycelium through oven dry, pulverize, be mixed, seasoning, packing, sterilization get product.
Among the above-mentioned preparation method, the semisynthetic medium of cultivating three kinds of slant strains is identical, the composition of substratum is by weight being expressed as, potato: wheat bran: glucose: peptone: yeast extract: potassium primary phosphate: sal epsom: agar=40: 4: 4: 0.4: 0.2: 0.2: 0.05: 4, and it is strong plain to add micro-mushroom therein, 18～28 ℃ of culture temperature, 5～15 days time.
Among the above-mentioned preparation method, three kinds of slant strains insert in the complex medium container with equivalent, the composition of its substratum is by weight being expressed as, wheat bran: Semen Maydis powder: analysis for soybean powder: sucrose: peptone: yeast extract: potassium primary phosphate: sal epsom=1: 1: 0.5: 2: 0.5: 0.2: 0.1: 0.05,20～28 ℃ of culture temperature, incubation time 7～10 days.
In the aforesaid method, the solid mycelium is cultivated, and the grain grain is 95% heavily, and connecing the bacterial classification amount is 5%, 20～28 ℃ of culture temperature, 4～5 days time.
Technical progress of the present invention is: adopt biotechnology, cultivate the solid mycelium, cost is low, technology is easy, and mycelial growth is fast, good quality and high output, be suitable for industrialized mass production, production process is carried out under aseptic condition, and batching does not add any sanitas and pigment.With the product cordycepic acid content 〉=200mg/100g of method of the present invention preparation, long-term edible, can make the body internal recycle be improved significantly, remove superoxide anion in the body, regulate human physiological functions, enhancing immunity ability, the purpose that can reach preventing disease and delay senility.
What Figure of description 1 provided is process flow sheet of the present invention.
The following examples are in order to explanation the present invention.
The mycelium of selecting for use Cordyceps strain, Grifola frondosa bacterial classification, Agaricus blazei to cultivate is a main raw material, and preparation Chinese caterpillar fungus health food comprises the steps:
---peacilomyce hepiahi belongs to bacterial strain, Grifola frondosa---No. 51 bacterial strain, Agaricus blazei Murrill---the Brazilian bacterial strain that a, utilizes rare edible medicinal fungus Cordyceps fungus, adopt high-tech healthy cell breeding method, according to genetic principle healthy cell is imported in the circulation breeding process, pick bad stay excellent, pure the strengthening of long-term maintenance bacterial classification do not degenerated, improve mushroom output, again pure strong mycelium is separated into the fritter bacterial classification with biotechnology, insert in the semisynthetic medium, under 18～28 ℃ of conditions, cultivated 5～15 days, promptly grow into the female kind in inclined-plane;
B, with the inclined-plane female plant to insert shake in bottle liquid nutrient medium, cultivated 7～10 days down at 20～28 ℃, promptly grow up to liquid spawn;
C, will shake bottle liquid spawn and insert again in ripe rice of boiling or the wheat natural medium, under thermophilic and aseptic condition, cultivate, cultivated 4～5 days by biotechnologies such as alternating temperature, logical oxygen, grain grain substratum promptly covers with mycelium, and grow the Chinese caterpillar fungus stroma, promptly cultivate successfully, dry, pulverization process.
D, add the seed of Job's tears, lotus seed, sesame etc. by the different flavor requirement and can make protective foodss such as Chinese caterpillar fungus lotus heart of a lotus seed powder, Chinese caterpillar fungus milk extract, the fine and soft electuary of Chinese caterpillar fungus, sterilization is an edible.
In above-mentioned preparation method, three kinds of different bacterial classifications are cultivated into slant strains (the female kind) at first respectively, and used semisynthetic medium is identical, and its composition is expressed as by weight:
Potato: wheat bran: glucose: peptone: yeast extract: potassium primary phosphate: sal epsom: agar=40: 4: 4: 0.4: 0.2: 0.2: 0.05: 4, and add strong element of micro-mushroom or vitamins B
1, 18～28 ℃ of culture temperature, 5～15 days time.
In present method, three kinds of slant strains (the female kind) equivalent inserts same shaking in the bottle and cultivates, and selects complex medium for use, and its composition is by weight ratio:
Wheat bran: Semen Maydis powder: analysis for soybean powder: sucrose: peptone: yeast extract: potassium primary phosphate: sal epsom=1: 1: 0.5: 2: 0.5: 0.2: 0.1: 0.05,20～28 ℃ of culture temperature, 7～10 days time.
Among this preparation method, send out bacterium with shaking in bottle rice that liquid spawn access boiling is ripe or the wheat broth, the proportion of its grain grain natural medium is 95%, and connecing the bacterial classification amount is 5%, 20～28 ℃ of culture temperature, 4～5 days time.
The Chinese caterpillar fungus health food that utilizes the present invention to prepare has fine and soft distinctive flavour of Chinese caterpillar fungus, Grifola frondosa, Ji Shu and smell, and sesame, lotus seed, the distinctive comprehensive fragrance of the seed of Job's tears, and sugariness is moderate, takes after mixing it with water evenly.After testing, nitrogen content 〉=0.48%, pH value 5.5～6.5, cordycepic acid content 200mg/100g.It is a kind of comparatively ideal protective foods.
Claims (4)
1, the preparation method of Chinese caterpillar fungus health food, selecting Cordyceps strain, Grifola frondosa bacterial classification, Agaricus blazei for use is raw material, it is characterized in that the preparation method comprises the steps:
A, the mycelium of above-mentioned three kinds of bacterial classifications is inserted in the semisynthetic medium respectively, cultivate into slant strains;
B, get above-mentioned three kinds of slant strains and insert in the same complex medium, cultivate into liquid spawn;
C, get liquid spawn and insert again in the malicious boiling that the disappeared ripe rice or wheat broth, cultivate into the solid mycelium;
D, with grain grain solid mycelium through oven dry, pulverize, be mixed, seasoning, packing, sterilize finished product.
2, press the preparation method of the described Chinese caterpillar fungus health food of claim 1, the semisynthetic medium that it is characterized in that cultivating three kinds of slant strains is identical, the composition of semisynthetic medium is expressed as by weight: potato: wheat bran: glucose: peptone: yeast extract: potassium primary phosphate: sal epsom: agar=40: 4: 4: 0.4: 0.2: 0.2: 0.05: 4, and it is strong plain to add micro-mushroom therein, 18 ℃～28 ℃ of culture temperature, 5～15 days time.
3, press the preparation method of the described Chinese caterpillar fungus health food of claim 1, it is characterized in that three kinds of slant strains equivalent are inserted in the container of same complex medium, cultivate into liquid spawn, the composition of complex medium is expressed as by weight, wheat bran: Semen Maydis powder: analysis for soybean powder: sucrose: peptone: yeast extract: potassium primary phosphate: sal epsom=1: 1: 0.5: 2: 0.5: 0.2: 0.1: 0.05,20～28 ℃ of culture temperature, incubation time 7～10 days.
4, by claim 1 or 2 or 3 described methods, it is characterized in that the cultivation of solid mycelium, grain grain weight is 95%, connecing the bacterial classification amount is 5%, 20～28 ℃ of culture temperature, 4～5 days time.
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| Application Number | Priority Date | Filing Date | Title |
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| CN98103039A CN1078051C (en) | 1998-07-21 | 1998-07-21 | Method for preparing health-care food contg. chinese caterpillar fungus |
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| CN98103039A CN1078051C (en) | 1998-07-21 | 1998-07-21 | Method for preparing health-care food contg. chinese caterpillar fungus |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002020727A3 (en) * | 2000-09-05 | 2003-01-16 | Globoasia Llc | Method for propagating fungi using solid state fermentation |
| WO2004064853A1 (en) * | 2001-07-30 | 2004-08-05 | Sichuan Dikang Industry Holdings Group Co., Ltd. | Cordyceps capsule and method for preparation thereof |
| CN1977865B (en) * | 2005-11-30 | 2010-10-06 | 广东省农业科学院农业生物技术研究所 | Method for preparing domestic fungus health care oral liquid |
| CN102160642A (en) * | 2011-03-17 | 2011-08-24 | 李佃场 | Method for preparing Cordyceps rice food |
| CN102172273A (en) * | 2011-03-09 | 2011-09-07 | 王永显 | Method for producing cordyceps militaris fermentation rice by utilizing solid state fermentation of rice |
| CN102178218A (en) * | 2011-03-17 | 2011-09-14 | 李佃场 | Solid fermentation method for Grifola frondosa and rice food |
| CN102578555A (en) * | 2011-12-27 | 2012-07-18 | 天津市林业果树研究所 | Edible fungi health maintenance powder and processing method thereof |
| CN103564421A (en) * | 2012-07-26 | 2014-02-12 | 天水众兴菌业科技股份有限公司 | Preparation method for paecilomyces bepiali chen powder |
| CN104207121A (en) * | 2014-07-07 | 2014-12-17 | 郑州乐嫒商贸有限公司 | Immunity-enhancing, blood-glucose-reducing, blood-pressure-reducing and blood-fat-reducing diet-therapy health-caring product |
| CN104770660A (en) * | 2014-01-13 | 2015-07-15 | 于汇 | Cordyceps sinensis rice with rich cordyceps militaris nutrients and rich medicinal values, and production process thereof |
| CN104823716A (en) * | 2015-05-08 | 2015-08-12 | 郭亚涛 | Culture and preparing method of fungus symbiotic hypha powder |
| CN105077028A (en) * | 2015-09-10 | 2015-11-25 | 即墨市第一职业中等专业学校 | Preparation method of cordyceps militaris health care rice |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100374543C (en) * | 2004-11-16 | 2008-03-12 | 中国科学院微生物研究所 | Semi-synthetic culture medium of cordyceps sineusis |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1078872A (en) * | 1993-05-20 | 1993-12-01 | 田子成 | Health drink prepared by mushroom double bacteria liquid fermentation method |
| CN1036531C (en) * | 1994-08-25 | 1997-11-26 | 俞永信 | Deep fermentation technology for cordyceps sinensis sacc |
| CN1148623A (en) * | 1996-10-04 | 1997-04-30 | 海南兆隆实业有限公司 | North cordyceps mycelium fermentation technology |
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1998
- 1998-07-21 CN CN98103039A patent/CN1078051C/en not_active Expired - Fee Related
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6558943B1 (en) | 2000-09-05 | 2003-05-06 | Sun Ten Pharmaceutical Co., Ltd. | Method for propagating fungi using solid state fermentation |
| WO2002020727A3 (en) * | 2000-09-05 | 2003-01-16 | Globoasia Llc | Method for propagating fungi using solid state fermentation |
| WO2004064853A1 (en) * | 2001-07-30 | 2004-08-05 | Sichuan Dikang Industry Holdings Group Co., Ltd. | Cordyceps capsule and method for preparation thereof |
| CN1977865B (en) * | 2005-11-30 | 2010-10-06 | 广东省农业科学院农业生物技术研究所 | Method for preparing domestic fungus health care oral liquid |
| CN102172273A (en) * | 2011-03-09 | 2011-09-07 | 王永显 | Method for producing cordyceps militaris fermentation rice by utilizing solid state fermentation of rice |
| CN102160642B (en) * | 2011-03-17 | 2012-07-04 | 李佃场 | Method for preparing Cordyceps rice food |
| CN102178218A (en) * | 2011-03-17 | 2011-09-14 | 李佃场 | Solid fermentation method for Grifola frondosa and rice food |
| CN102178218B (en) * | 2011-03-17 | 2012-06-27 | 李佃场 | Solid fermentation method for Grifola frondosa and rice food |
| CN102160642A (en) * | 2011-03-17 | 2011-08-24 | 李佃场 | Method for preparing Cordyceps rice food |
| CN102578555A (en) * | 2011-12-27 | 2012-07-18 | 天津市林业果树研究所 | Edible fungi health maintenance powder and processing method thereof |
| CN103564421A (en) * | 2012-07-26 | 2014-02-12 | 天水众兴菌业科技股份有限公司 | Preparation method for paecilomyces bepiali chen powder |
| CN104770660A (en) * | 2014-01-13 | 2015-07-15 | 于汇 | Cordyceps sinensis rice with rich cordyceps militaris nutrients and rich medicinal values, and production process thereof |
| CN104207121A (en) * | 2014-07-07 | 2014-12-17 | 郑州乐嫒商贸有限公司 | Immunity-enhancing, blood-glucose-reducing, blood-pressure-reducing and blood-fat-reducing diet-therapy health-caring product |
| CN104207121B (en) * | 2014-07-07 | 2016-01-06 | 郑州乐嫒商贸有限公司 | A kind of immunity that improves reduces by three high food therapeutic healths |
| CN104823716A (en) * | 2015-05-08 | 2015-08-12 | 郭亚涛 | Culture and preparing method of fungus symbiotic hypha powder |
| CN105077028A (en) * | 2015-09-10 | 2015-11-25 | 即墨市第一职业中等专业学校 | Preparation method of cordyceps militaris health care rice |
| CN105077028B (en) * | 2015-09-10 | 2018-10-16 | 即墨市第一职业中等专业学校 | A kind of preparation method of Cordceps militaris health-care rice |
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| CN1078051C (en) | 2002-01-23 |
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