CN101513172B - Method for propagating tridentiger trigonocephalus with artificial comprehensive regulation ecological factors - Google Patents
Method for propagating tridentiger trigonocephalus with artificial comprehensive regulation ecological factors Download PDFInfo
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- CN101513172B CN101513172B CN200810033701XA CN200810033701A CN101513172B CN 101513172 B CN101513172 B CN 101513172B CN 200810033701X A CN200810033701X A CN 200810033701XA CN 200810033701 A CN200810033701 A CN 200810033701A CN 101513172 B CN101513172 B CN 101513172B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention provides a method for propagating tridentiger trigonocephalus with artificial comprehensive regulation ecological factors and relates to a method for propagating tridentiger trigonocephalus with regulation ecological factors. The method is to put parent fish in a spawning box, and is characterized in that: the ratio of the female parent fish to the male parent fish is 1:1 and each pair of fishes takes up an area of 0.25 m<2> in the spawning box; the spawning box is provided with holes with rough inner walls; the temperature of the water in the spawning box is 18 to 21 DEG C, with a 0.3 DEC C rise each day; the salinity of the water is 2 to 6; the concentration of dissolved oxygen in the water is 3.5 to 6.5mg/ml; the pH value of the water is 7.5 to 9.0; 8-hour illumination and 16-hour darkness are provided for the propagation environment each 24 hours, with an illumination intensity less than 600Lx; the environmental noise level is less than 15 dcibels; the bottom and the four sides of the spawning box are black; the female fish are taken out of an incubator after spawning, the flow rate of the water in the incubator is 2 to 4 cm/s, one third of the water is changed each day, the incubation lasts for 7 to 11 days; and the fish fries coming out of membrane are transferred to a nursery box till the propagation process completes. The method is used for artificial propagation of tridentiger trigonocephalus.
Description
Technical field:
The present invention relates to the artificial fecundation method of line thin white silk used in ancient China goby.
Background technology
Wen Gao Acenthogobius flavimanus (Tridentiger trigonocephalus) belongs to Perciformes, and ((Gobiidae) Gao Acenthogobius flavimanus belongs to (Tridentiger), and this fish happiness is lived in caves, and is food with shrimp, small fish etc., and the male and female individuality has notable difference in Perciformes) Acenthogobius flavimanus section.Acenthogobius flavimanus section (Gobiidae) fish kind number is more, most kind individualities are little, be littoral eurysalinity benthic fishes, distributed more widely, mainly be distributed in the pacific rim of northeast, Asia, being mainly seen in waters such as the yellow Bohai Sea, the East Sea, the South Sea and the Taiwan Straits in China, also is the common small fishes in low salinity zone, entrance of Changjiang River.Reproductive biology research to Acenthogobius flavimanus section at present mainly comprises reports such as individual reproduction power, reproductive habit and colony form, the early stage history of life, furthers investigate considerably less.
Zhao Bin, Fan Liping, people such as Wang Jian are in the breeding situation of the multiple goby of report lance tail on " fishery modernization " periodical in 2005, rely on the multiple goby parent population of lance tail of natural maturation, in the pond natural spawning and hatch fry after, the bait such as soya-bean milk of manually throwing something and feeding are fished for the fry in the pond then.This breeding is the breeding under the natural conditions, and its parent is the parent population of physical maturity in the natural surroundings.Do not see at present the report of relevant Wen Gao Acenthogobius flavimanus artificial propagation techniques.
At present in the artificial propagation of fish seed, directly by regular injection exogenous hormone, the maturation that promotes fish with lay eggs, just take manually crowded essence and egg-fetching method after arriving effector phase, then smart ovum is blended in one and carries out artificial insemination.First generation artificial induced spawning propagation technique is to use fish hypophysis gonadotropin, or the blastodisc human chorionic gonadtropin that extracts from people's urine; Second generation artificial induced spawning propagation technique is the using artificial synthetic, comprises LHRH analog, GRIF etc.In the artificial propagation as mudskipper, at first adopting the injection hormone to accelerate the ripening, mainly is LHRH-A3 and HCG.Per injection dosage is LHRH-A3 1ug+HCG 100IU/ tail, and male and female parent population Isodose, injection site are the pectoral fin base portion.After accelerating the ripening, adopt semi-dry process to carry out artificial insemination, promptly extrude female fish egg earlier and check maturity, choose the measured ovum of matter, dissect milter simultaneously and take out spermary, be cut into small pieces, place in the mortar and grind, add a little sea-water activated sperm, then seminal fluid is drenched on ovum, stirring allows it fully mix fertilization, then with the clean sea water washing several times, moves in the incubation vessel and hatches.Such artificial fecundation method not only labour intensity is big, the specification requirement height, and the smart archiblast amount of adopting must be got well, and more to the number of operations of fish, and fish are got a fright, and causes the injured infection of parent population dead after having an injection easily.
After fish reach sexual maturity, being subjected to the condition factors such as natural illumination, temperature, salinity, pH value, dissolved oxygen in mating season stimulates, and its gonad development can transform to the V phase from the IV phase, and under chasing mutually of female milter flirted stimulation, begin to lay eggs, finish the natural propagation process.As utilize the prolongation of illumination, the fish that lays eggs spring is laid eggs in advance, with short illumination the fish that lays eggs autumn is laid eggs in advance, the someone adopts and replenishes illumination and come the extended day photograph, successfully makes laying eggs of the river salmon that is in the aquarium and rainbow salmon do sth. in advance 3～4 months than the egg-laying period of occurring in nature.Carp generally requires water temperature to lay eggs in the time of 18 ℃, if ovary is fully grown, but and also do not reach when laying eggs 18 ℃ of temperature thresholds when water temperature, laying eggs of carp just can not realize.If but manually create conditions when improving water temperature and reaching more than 18 ℃, carp also can lay eggs in the winter time.Therefore in natural surroundings, impelling fish gonad maturity and the environmental factor of laying eggs, is not to be single, but the comprehensive function of some factors just becomes the problem that needs solve but how to regulate and control these composite factors.
Line thin white silk used in ancient China Acenthogobius flavimanus carries out taking 1. to have an injection in the artificial propagation conventional method to hasten parturition: inject the medicament of hastening parturition, less because of fish, this method causes the injured death of parent population easily; 2. directly carry out artificial insemination: after the parent population of catching is screened, directly take out sperm and ovum and carry out artificial insemination, relatively poor because of piscinity gland degree of reaching maturity, exist and grow nonsynchronous problem, make the second-rate of fertilized egg, lethality is higher when causing hatching.Show that by Wen Gao Acenthogobius flavimanus having been carried out the artificial propagation techniques result of study breeding period of Wen Gao Acenthogobius flavimanus is longer, maturity of fish gonads disunity, sperm and ovum exist grows nonsynchronous problem, and raun is more in the parent population of catching, and milter is less.Need to create a series of suitable ecotopes, impel it to lay eggs, save manpower and material resources, avoid the defective that exists in aforementioned 2 kinds of methods, just can reach reproductive effect preferably.
Summary of the invention
The technical issues that need to address of the present invention provide a kind of propagation method of tridentiger trigonocephalus with artificial comprehensive regulation ecological factors.
Technical scheme of the present invention is included in fishes for parent population the mating season of Wen Gao Acenthogobius flavimanus, and the breeding spot is transported in oxygenation back, chooses gonad development parent population preferably, is placed in the case of laying eggs
The female-male proportion of putting into parent population in the case of laying eggs is 1: 1, and every pair of parent population takies the case area of laying eggs at 0.25m
2More than; The cave that laying eggs has in the case tile to build up pierces for Wen Gao Acenthogobius flavimanus parent population wherein carries out spawning and fertilization; Each case will guarantee that cave quantity is no less than the logarithm of parent population, in order to avoid parent population is fought for the cave, the inwall in cave must be coarse, hatches so that ovum is bonded on the inwall, and the coarse fiber of bottom shop one deck of the case of laying eggs so that some ovum is bonded on the fiber is hatched; The breeding water body ecological condition control of case of laying eggs is as follows: in 18～21 ℃ of scopes of water temperature, improve 0.3 ℃ every day gradually; Salinity maintains in 2～6 scopes; Dissolved oxygen keeps 3.5～6.5mg/ml; PH maintains in 7.5～9.0 scopes; The environmental ecology condition control of breeding is as follows: reduce light application time, reduce intensity of illumination, each takes illumination in 8 hours round the clock, 16 hours dark modes; Intensity of illumination is controlled at below the 600Lx; Reduce noise, the minimizing personnel scaring of walking about is kept environmental noise below 15 decibels as far as possible; In the bottom of the case of laying eggs with spread black ceramic tiles all around, the top keeps the black environment with the shading of sunshade net; After raun lays eggs it is shifted out incubator box, milter is then kept on the fertilized egg side and protects, and milter can be takeed good care of fish-egg every day meticulously, and manually form certain flow condition this moment in incubator box, remain on 2～4cm/s; After this notice that not startling milter protects ovum, only need change the water about 1/3rd every day, brooding time 7～11 days; Behind the fry membrane, pull fry out with net, change in the seedling growing box and cultivate, promptly finish the artificial ecology reproductive process of seed.
The present invention utilizes the physiology and the ecosystem characterization of Wen Gao Acenthogobius flavimanus, create the environmental condition that is fit to its breeding, method by ecological factors such as regulating and controlling temperature, salinity, dissolved oxygen, pH, illumination, substrate, current, reach physical maturity, oestrus, lay eggs, be fertilized, hatch, have the rate of accelerating the ripening, fertilization rate and the emergence rate that improve parent population, reduce the lethality of parent population, reduce the advantage of technical difficulty, make the propagation method of Wen Gao Acenthogobius flavimanus realize breaking through first by artificial comprehensive regulation.
Embodiment
Fish for line thin white silk used in ancient China Acenthogobius flavimanus parent population in mating season of line thin white silk used in ancient China Acenthogobius flavimanus (waters, entrance of Changjiang River 4～May during), the breeding spot is transported in oxygenation back, chooses gonad development parent population preferably, is placed in the case of laying eggs.The discrimination method of parent population gonad development is: the belly of raun expands outstanding, and the gonopore evagination takes on a red color, and gently squeezes belly, and ovum can flow out smoothly; The pectoral fin of milter is glassy yellow, and obvious black splotch is arranged on pectoral fin.The female-male proportion of putting into parent population in the case of laying eggs is 1: 1, and every pair of parent population takies the case area of laying eggs at 0.25m
2More than; In 5 square metres of cement pits, put into 20 pairs of Wen Gao Acenthogobius flavimanus parent populations, promptly put into the male Wen Gao of the female Wen Gao of 20 tails Acenthogobius flavimanus parent population and 20 tails Acenthogobius flavimanus parent population.The cave that has 25 tiles to build up in the cement pit can pierce wherein for line thin white silk used in ancient China Acenthogobius flavimanus parent population, and carry out spawning and fertilization.The lay eggs coarse fiber of bottom shop one deck of case so that some ovum is bonded on the fiber is hatched.The control of cement pit breeding water body ecological condition is as follows: 18～21 ℃ of water temperatures, improve 0.3 ℃ every day gradually; Salinity maintains in 2～6 scopes; Dissolved oxygen keeps 3.5～6.5mg/ml; PH maintains in 7.5～9.0 scopes.Each takes illumination in 8 hours round the clock, 16 hours dark modes; Intensity of illumination is controlled at below the 600Lx; Reduce noise, the minimizing personnel scaring of walking about is kept environmental noise below 15 decibels as far as possible; In the bottom of cement pit with spread black ceramic tiles all around, the top keeps the black environment with the shading of sunshade net.After this female milter exchanges in the cave mutually by constantly passing in and out the cave, and successful matching in the cave at last can be laid eggs in the cave.It can be shifted out incubator box after raun lays eggs, milter is then kept on the fertilized egg side and protects, and milter can be takeed good care of fish-egg every day meticulously.This moment is the certain flow condition of artificial formation in incubator box, remains on 2～4cm/s.After this notice that not startling milter protects ovum, only need change the water about 1/3rd every day, brooding time 7～11 days.Behind the fry membrane, pull fry out with net, change in the seedling growing box and cultivate, promptly finish the artificial ecology reproductive process of seed.
Claims (1)
1. the propagation method of tridentiger trigonocephalus with artificial comprehensive regulation ecological factors, fish for parent population the mating season that is included in line thin white silk used in ancient China goby, the breeding spot is transported in oxygenation back, choose gonad development parent population preferably, be placed in the case of laying eggs, the female-male proportion of putting into parent population in the case that it is characterized in that laying eggs is 1: 1, and every pair of parent population takies the case area of laying eggs at 0.25m
2More than; The cave that laying eggs has in the case tile to build up pierces for line thin white silk used in ancient China goby parent population wherein carries out spawning and fertilization; Each case of laying eggs will guarantee that cave quantity is no less than the logarithm of parent population, in order to avoid parent population is fought for the cave, the inwall in cave must be coarse, hatches so that ovum is bonded on the inwall, and the coarse fiber of bottom shop one deck of the case of laying eggs so that some ovum is bonded on the fiber is hatched; The breeding water body ecological condition control of case of laying eggs is as follows: in 18～21 ℃ of scopes of water temperature, improve 0.3 ℃ every day gradually; Salinity maintains in 2～6 scopes; Dissolved oxygen keeps 3.5～6.5mg/ml; PH maintains in 7.5～9.0 scopes; The environmental ecology condition control of breeding is as follows: reduce light application time, reduce intensity of illumination, each takes illumination in 8 hours round the clock, 16 hours dark modes; Intensity of illumination is controlled at below the 600Lx; Reduce noise, the minimizing personnel scaring of walking about is kept environmental noise below 15 decibels as far as possible; In the bottom of the case of laying eggs with spread black ceramic tiles all around, the top keeps the black environment with the shading of sunshade net; After raun lays eggs it is shifted out the case of laying eggs, milter is then kept on the fertilized egg side and protects, and milter can be takeed good care of fish-egg every day meticulously, and manually form certain flow condition this moment in the case of laying eggs, remain on 2～4cm/s; After this notice that not startling milter protects ovum, only need change the water about 1/3rd every day, brooding time 7～11 days; Behind the fry membrane, pull fry out with net, change in the seedling growing box and cultivate, promptly finish the artificial ecology reproductive process of seed.
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| CN101513172B true CN101513172B (en) | 2011-04-20 |
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| CN102835340B (en) * | 2012-09-24 | 2013-08-21 | 厦门大学 | Breeding method of Rillengrundel |
| CN103718998B (en) * | 2013-12-18 | 2018-04-27 | 上海市水产研究所 | A kind of method for obtaining Synechogobius ommaturus ecology early-breeding seedlings kind |
| CN105409844A (en) * | 2015-11-28 | 2016-03-23 | 广东海洋大学 | Warning method for toxic gobies |
| CN114375879B (en) * | 2021-12-29 | 2023-07-18 | 广东省鳇鲸海洋生物科技有限公司 | Cultivation method for promoting gonad development of parent yellow lip fish |
| CN115500288B (en) * | 2022-09-30 | 2023-08-11 | 广西宇健农业科技有限公司 | Breeding and seedling selecting method for largemouth black weever fries |
Citations (1)
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| CN101032231A (en) * | 2006-03-09 | 2007-09-12 | 上海能正渔业科技开发有限公司 | Techniques for the artificial propagation and larval rearing of fugu obscurus |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101032231A (en) * | 2006-03-09 | 2007-09-12 | 上海能正渔业科技开发有限公司 | Techniques for the artificial propagation and larval rearing of fugu obscurus |
Non-Patent Citations (5)
| Title |
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| JP特开2002-330706A 2002.11.19 |
| 严志云等.抚仙湖子陵吻鰕虎鱼繁殖策略的可塑性研究.《水生生物学报》.2007,第31卷(第3期),414-418. * |
| 李建军等.裸项栉鰕虎鱼的生物学特性研究.《中国比较医学杂志》.2007,第17卷(第1期),39-41. * |
| 赵斌等.矛尾复鰕虎鱼生物学繁养殖技术初报.《渔业现代化》.2005,(第6期),26-27. * |
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