CN100435641C - Method for cotton bough cuttage and its special cuttage rooting agent - Google Patents

Method for cotton bough cuttage and its special cuttage rooting agent Download PDF

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Publication number
CN100435641C
CN100435641C CNB2005100889628A CN200510088962A CN100435641C CN 100435641 C CN100435641 C CN 100435641C CN B2005100889628 A CNB2005100889628 A CN B2005100889628A CN 200510088962 A CN200510088962 A CN 200510088962A CN 100435641 C CN100435641 C CN 100435641C
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China
Prior art keywords
cotton
root
branch cutting
cotton branch
growing agent
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Expired - Fee Related
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CNB2005100889628A
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Chinese (zh)
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CN1907031A (en
Inventor
李付广
张朝军
李凤莲
王玉芬
武芝侠
刘传亮
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Henan Zhongyuan middle breed improved breed Co., Ltd.
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Institute of Cotton Research of Chinese Academy of Agricultural Sciences
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  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a cotton bough cottage method and special-purpose root-growing agent for cottage, wherein each 1000mL of the water solution of the agent contains the following substances, KI0.004-0.02g, MnSO4*4H2O 0.017-0.085g, HBO3 0.003-0.012g, ZnSO4*7H2O 0.0043-0.035g, IAA 10-30mg, KT 1-10mg, and 6-BA 1-10mg. The powder preparation of the agent comprises KI, MgSO4*4H2O, HBO3, ZnSO4*7H2O, IAA, KT and 6-BA by the weight ratio of 4-20:17-85:3-12:4.3-35:10-30:1-10:1-10.

Description

One grow cotton branch cutting method and special-purpose cuttage root-taking agent thereof
Technical field
The invention belongs to one in the agricultural technology field grow cotton branch cutting method and special-purpose cuttage root-taking agent thereof.
Background technology
In cotton breeding work, the seed selection of new cotton variety finally is that the selection final election by fine individual plant obtains, and the selections of phase for a long time such as seedling stage, flower bud phase, flowering and boll-setting period, the term of opening bolls are mainly passed through in the selection of cotton fine individual plant, finally determine fine individual plant.Because cotton is the cross pollination crop, there is serious genetic variation in the seed of fine individual plant, and the person that therefore needs the breeding work carries out for many years pedigree and selects just might obtain the strain system close with selected individual plant proterties.Not only can preserve fine individual plant and use cotton branch cutting technology, individual plant can also be expanded microcommunity of numerous one-tenth, improve the accuracy of selecting.
Cotton is the same with other annual crop, and the genetic variation of improved seeds, degeneration are one of difficult problems of puzzlement breeding circle.The method that solves this difficult problem normally by the breeder purify, rejuvenation.Therefore, annual all need the professional and technical personnel to drop into a large amount of energy, but actual effect is unsatisfactory, for example: the kind of once winning the cotton institute 12 of cotton variety of national first class invetion award does not just obtain complete preservation.The good characteristic of cotton material can be along with increase a large amount of variations with expanding numerous, the degeneration of reproductive order of generation.This is because biological person's character determines.How to solve breeding is the research emphasis that plant germplasm resource is preserved with the contradiction of preserving always.Cotton branch cutting technology can thoroughly solve this contradiction.
The application of cotton crossbreed mainly is subjected to the restriction of seeding technique.The cotton seed production technology mainly adopts the method for artificial emasculation hybridization at present, and because of the amount of labour used is big, the seed costs height makes crossbreed on the high side.The effect of utilizing cotton branch cutting technology can produce a production of hybrid seeds, use for many years.
But because the branch cutting of cotton is easy to take root not as woody plant like that, cause survival rate very low, be not applied always.
Summary of the invention
The purpose of this invention is to provide grow cotton a branch cutting method and a special-purpose cuttage root-taking agent thereof.
The cotton branch cutting provided by the present invention aqua of taking root contains following substances: KI0.004-0.02g, MnSO in every 1000mL water 44H 2O 0.017-0.085g, HBO 30.003-0.012g, ZnSO 47H 2O0.0043-0.035g, IAA 10-30mg, KT 1-10mg, 6-BA 1-10mg.
The preferred cotton branch cutting aqua prescription of taking root is to contain following substances in every 1000mL water: KI 0.005g, MnSO 44H 2O 0.045g, HBO 30.007g, ZnSO 47H 2O 0.015g, IAA 15mg, KT 5mg, 6-BA 8mg.
For obtaining rooting efficiency preferably, above-mentioned cotton branch cutting is taken root and also is added with trace mineral supplement in the aqua, comprises in the following trace mineral supplement one or more: VB 10.2-0.8mg, VB 60.2-0.8mg and inositol 75-150mg; Be preferably: VB 10.4mg, VB 60.4mg and inositol 100mg.
For instant packed, also cotton branch cutting root-growing agent of the present invention can be made pulvis.The described cotton branch cutting pulvis of taking root includes KI, the MnSO that ratio of weight and number is 4-20: 17-85: 3-12: 4.3-35: 10-30: 1-10: 1-10 44H 2O, HBO 3, ZnSO 47H 2O, IAA, KT and 6-BA.
It is 12: 51: 7 that preferred cotton branch cutting root-inducing powder agent prescription includes ratio of weight and number: 20: 20: 5: 5 KI, MnSO 44H 2O, HBO 3, ZnSO 47H 2O, IAA, KT and 6-BA.
The cotton branch cutting is taken root and also can be added with the VB that ratio of weight and number is 0.2-0.8: 0.2-0.8: 75-150 in the pulvis 1, VB 6Or/and one or more trace mineral supplements in the inositol.
If adopt the formulation of pulvis, before use, only need it is soluble in water by a certain percentage, wiring solution-forming gets final product.
Root-growing agent of the present invention can adopt the conventional method preparation.
Second purpose of the present invention provides the above-mentioned cotton branch cutting of a kind of usefulness root-growing agent to carry out the method for cotton branch cutting.
The method of cotton branch cutting provided by the present invention is that cuttage was added a cover the plastic film heat and moisture preserving and cultivated after 25-40 days in seedling medium, can transplant after the base portion of cotton branch was soaked 20-28 hour with above-mentioned cotton branch cutting root-growing agent.Wherein, the preferred root-growing agent processing time is 24 hours, and raffinate can water on the cutting medium.
In actual applications, be the expansion face of taking root, can be with the wedge shape that is whittled into of branch base portion, being beneficial to root is constantly stimulated and is taken root, and reaches the effect in strong root, strong sprout.
The cotton branch that available said method carries out the cuttage cultivation is fruit branch, vegetative shoot, superfluous bud or the stem etc. of cotton.
The invention provides a kind of root-growing agent that the cotton branch cutting is taken root that is suitable for, is to be host with plant growth regulator with the micronutrient element compound relevant with root development, the pulvis or the solution that obtain by certain technology and additive compound.The present invention can shorten between the root differentiation phase when the cotton branch cutting, improves the amount of taking root, rooting rate and the cuttage survival rate of cotton branch, and survival rate can reach more than 95%; Thoroughly solved the problem that cotton branch cutting technology exists in application, helped cotton branch cutting technology, will in soilless-cultivated seedling, factorial seedling growth and the germ plasm resource preservation of cotton, play a significant role scientific research and applying of producing.
Embodiment
Method therefor is conventional method if no special instructions among the following embodiment.
The preparation of embodiment 1, cotton branch cutting root-growing agent
Take by weighing following material: KI 0.005g, MnSO 44H 2O 0.05g, HBO 30.009g, ZnSO 47H 2O0.02g, IAA 20mg, KT 5mg, 6-BA 5mg, trace mineral supplement: VB 10.4mg, VB 60.4mg, inositol 100mg.Be dissolved in 1 liter of distilled water above-mentioned substance standby.
The test of taking root of embodiment 2, cotton branch cutting
The influence that the cotton branch cutting root-growing agent of check embodiment 1 preparation is taken root to the cotton branch, concrete grammar is: select the healthy and strong anosis cotton branch that has 2-3 sheet true leaf for use, base portion is whittled into the wedge shape that is about 1cm, immerses in the root-growing agent of embodiment 1 preparation, soaked 24 hours.Then on the Cultivation of Cotton Seedlings Without Soil medium of cuttage in the plastic casing of growing seedlings (being convenient to get root observes) (this culture medium prescription is referring to the patent " a kind of Cultivation of Cotton Seedlings Without Soil matrix " of The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute's cultivating chamber, number of patent application: 03149367.X), water on the above-mentioned seedling medium after 50 times of the raffinate of the used root-growing agent dilutions, add a cover plastic film insulation, the cultivation of preserving moisture, the cotton branch of contrast for not handling through root-growing agent, this test repeats twice; A month " Invest, Then Investigate " seeding quality utilizes MRS-2400TPFU scanner scanning Levant Cotton Root length, root surface area, and utilizes DT-scan software to carry out data and handle, and the result is as shown in table 1, and contrast only detects a branch that survives.
Table 1 detects the result that cotton branch cutting root-growing agent is taken root and influenced branch
Survival rate/% Root dry weight/g Total root length/mm Root surface area/mm 2
Contrast 13.2 0.01 32.6 21.3
The branch cutting root-growing agent is handled plant 95.80 0.02 372.7 146.8
Table 1 data show, use cotton branch cutting root-growing agent that the survival rate of regrowth is brought up to more than 95%, and root dry weight increases by 4 times, and the root length overall is 11.4 times of contrast, and root surface area is 6.89 times of contrast, and rooting effect is obvious.

Claims (10)

1, the branch cutting aqua of taking root that grows cotton contains following substances: KI 0.004-0.02g, MnSO in every 1000mL water 44H 2O 0.017-0.085g, HBO 30.003-0.012g, ZnSO 47H 2O 0.0043-0.035g, IAA10-30mg, KT 1-10mg, 6-BA 1-10mg.
2, the cotton branch cutting according to claim 1 aqua of taking root is characterized in that: contain following substances KI 0.005g, MnSO in described every 1000mL water 44H 2O 0.045g, HBO 30.007g, ZnSO 47H 2O 0.015g, IAA 15mg, KT 5mg, 6-BA 8mg.
3, the cotton branch cutting according to claim 1 and 2 aqua of taking root is characterized in that: be added with in the following trace mineral supplement one or more: VB in the described cotton branch cutting root-growing agent 10.2-0.8mg, VB 60.2-0.8mg and inositol 75-150mg.
4, the branch cutting pulvis of taking root that grows cotton includes KI, MnSO that ratio of weight and number is 4-20: 17-85: 3-12: 4.3-35: 10-30: 1-10: 1-10 44H 2O, HBO 3, ZnSO 47H 2O, IAA, KT and 6-BA.
5, the cotton branch cutting according to claim 4 pulvis of taking root is characterized in that: described cotton branch cutting is taken root, and to include ratio of weight and number be 12: 51: 7 to pulvis: 20: 20: 5: 5 KI, MnSO 44H 2O, HBO 3, ZnSO 47H 2O, IAA, KT and 6-BA.
6, according to claim 4 or the 5 described cotton branch cuttings pulvis of taking root, it is characterized in that: be added with the VB that ratio of weight and number is 0.2-O.8: 0.2-0.8: 75-150 in the described cotton branch cutting root-growing agent 1, VB 6And inositol.
7, a kind of method of carrying out the cotton branch cutting with the arbitrary described cotton branch cutting root-growing agent of claim 1-6, be after the base portion of cotton branch is soaked 20-28 hour with above-mentioned cotton branch cutting root-growing agent, cuttage is added a cover the cultivation of plastic film heat and moisture preserving and is transplanted after 25-40 days in seedling medium.
8, method according to claim 7 is characterized in that: the described root-growing agent processing time is 24 hours.
9, method according to claim 7 is characterized in that: before handling with root-growing agent, the base portion with the cotton branch is whittled into wedge shape earlier.
10, according to claim 7 or 8 or 9 described methods, it is characterized in that: the fruit branch that described cotton branch is a cotton, vegetative shoot, superfluous bud or stem.
CNB2005100889628A 2005-08-03 2005-08-03 Method for cotton bough cuttage and its special cuttage rooting agent Expired - Fee Related CN100435641C (en)

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CN100435641C true CN100435641C (en) 2008-11-26

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Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102550605B (en) * 2012-01-05 2013-10-30 南京轩凯生物科技有限公司 Gamma-polyglutamic acid and naphthylacetic acid combined type rooting agent and using method thereof
CN106376619A (en) * 2016-08-31 2017-02-08 山东胜伟园林科技有限公司 Rooting agent containing ferric citrate and preparation method thereof
CN107006247A (en) * 2017-03-24 2017-08-04 合肥云都棉花有限公司 One cottage method grown cotton
CN106856953A (en) * 2017-03-24 2017-06-20 合肥云都棉花有限公司 One grows cotton cuttage root-taking matrix and its cottage method
CN111685123A (en) * 2020-07-17 2020-09-22 深圳万禾农业科技有限公司 Plant growth regulator composition and preparation method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6072808A (en) * 1983-08-23 1985-04-24 チバ−ガイギ− アクチエンゲゼルシヤフト Plant growth regulation
WO1999063819A1 (en) * 1998-06-08 1999-12-16 Donlar Corporation Plants grown utilizing poly (organic acid) as a nutrient absorption enhancer
CN1504088A (en) * 2002-12-03 2004-06-16 中国农业科学院棉花研究所 Root inducing agent and its preparation and use

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6072808A (en) * 1983-08-23 1985-04-24 チバ−ガイギ− アクチエンゲゼルシヤフト Plant growth regulation
WO1999063819A1 (en) * 1998-06-08 1999-12-16 Donlar Corporation Plants grown utilizing poly (organic acid) as a nutrient absorption enhancer
CN1504088A (en) * 2002-12-03 2004-06-16 中国农业科学院棉花研究所 Root inducing agent and its preparation and use

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