CN100434442C - Method for enriching beta glucan from highland barley - Google Patents
Method for enriching beta glucan from highland barley Download PDFInfo
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- CN100434442C CN100434442C CNB2006100430965A CN200610043096A CN100434442C CN 100434442 C CN100434442 C CN 100434442C CN B2006100430965 A CNB2006100430965 A CN B2006100430965A CN 200610043096 A CN200610043096 A CN 200610043096A CN 100434442 C CN100434442 C CN 100434442C
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- 235000007340 Hordeum vulgare Nutrition 0.000 title claims abstract description 99
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 title claims abstract description 43
- 229920002498 Beta-glucan Polymers 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 17
- 240000005979 Hordeum vulgare Species 0.000 title 1
- 241000209219 Hordeum Species 0.000 claims abstract description 98
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000001035 drying Methods 0.000 claims abstract description 8
- 235000015099 wheat brans Nutrition 0.000 claims description 35
- 239000012535 impurity Substances 0.000 claims description 14
- 235000013339 cereals Nutrition 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 241000209140 Triticum Species 0.000 claims description 7
- 235000021307 Triticum Nutrition 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- 239000004575 stone Substances 0.000 claims description 7
- 238000012856 packing Methods 0.000 claims description 6
- 238000001125 extrusion Methods 0.000 claims description 4
- 230000001007 puffing effect Effects 0.000 claims description 4
- 239000002699 waste material Substances 0.000 abstract description 5
- 238000000605 extraction Methods 0.000 abstract description 3
- 239000003960 organic solvent Substances 0.000 abstract description 3
- 239000002904 solvent Substances 0.000 abstract description 2
- 238000010298 pulverizing process Methods 0.000 abstract 2
- 229940098396 barley grain Drugs 0.000 abstract 1
- 238000004806 packaging method and process Methods 0.000 abstract 1
- 238000000053 physical method Methods 0.000 abstract 1
- 239000000843 powder Substances 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 10
- 230000000694 effects Effects 0.000 description 7
- 235000012000 cholesterol Nutrition 0.000 description 5
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 230000000259 anti-tumor effect Effects 0.000 description 3
- 210000000941 bile Anatomy 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000222336 Ganoderma Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 238000009461 vacuum packaging Methods 0.000 description 2
- 229920000018 Callose Polymers 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 201000010989 colorectal carcinoma Diseases 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000036186 satiety Effects 0.000 description 1
- 235000019627 satiety Nutrition 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention provides a physical method for enriching beta-glucan in highland barley, which comprises the processing steps of highland barley grain drying, pulverization, highland barley bran separation, superfine comminution, bulking, pulverization, packaging, etc. Tests show that the content of beta-glucan in highland barley bran fine powder prepared by the method of the present invention can reach 28 to 35%. As the present invention enriches the beta-glucan in highland barley in a physical mode without any solvent, no organic solvent is left in beta-glucan extract, and meanwhile, both water soluble and insoluble beta-glucan in the highland barley can be enriched. Loss and waste caused by that the water insoluble beta-glucan flows away with waste material in other extraction methods are avoided.
Description
Technical field
The present invention relates to a kind of preparation technology of beta-glucan, relate in particular to a kind of method of from highland barley, extracting beta-glucan.
Background technology
Studies show that beta-glucan has blood sugar regulation, improves immunizing power, antitumor etc., and present biomedical boundary of effect and nutrition educational circles generally believe that the physiological action of beta-glucan mainly contains 4 aspects:
The gut purge effect: beta-glucan is as food fibre, and high molecular is occupied certain volume in the digestion organs of human body, be difficult to be digested, can help the wriggling of stomach and intestine, thereby promote the drainage of intestinal toxic thing, the factor of curing the disease in the minimizing intestines contacts with intestinal mucosa, prevents colorectal carcinoma indirectly.
Blood sugar regulation: beta-glucan is the carbohydrate of non-starch based, itself is low sugar, low in calories, and its coarse fiber structure can delay the release of blood sugar again, increases satiety, stablizes the blood sugar effect thereby play.Help the auxiliary dietotherapy of prevent diabetes or diabetic subject.
Reducing cholesterol: what beta-glucan can reducing cholesterol is synthetic.Research thinks that this may be because beta-glucan has the effect that absorbs bile acide and impel bile acide to excrete, and promotes that cholesterol transforms to bile acide, effectively suppresses the rising of cholesterol in serum.The convenient effect of beta-glucan simultaneously also helps the cholesterol in the food not have to stop before absorbing just to be drained.
Improve immunizing power: beta-glucan can combine with scavenger cell among the human immune system, strengthens macrophage activity and phagocytic activity, thereby strengthens the resistance against diseases of human body.Some experts think that this function of beta-glucan is to studying its antitumous effect highly significant.The ganoderan that contains in the modern study discovery glossy ganoderma, major part all is a dextran, this also is that glossy ganoderma has one of important substance basis of immunomodulatory, anti-tumor function.
According to the data introduction, Tibet highland barley new variety cereal glucan content is very abundant, and in dark blue 25 the highland barley new variety, beta-glucan content is up to 8.62%.Beta-glucan is present in hullessbarley seed endosperm, the gluten cell wall in the highland barley, and its chemical name is: (1-3) (1-4)-callose is a kind of non-starch polysaccharide, can be water-soluble and water-insoluble two classes by its solvability branch.The preparation method of existing beta-glucan mostly be chemical method, the water-soluble beta-glucan of Enzymatic Extraction, and the water-insoluble beta-glucan discards with waste material, and more or less use organic solvent in extraction, causes organic solvent residual in extract.
Summary of the invention
The purpose of this invention is to provide a kind of method by beta-glucan (comprising water-soluble beta-glucan and water-insoluble beta-glucan) in the mode enrichment highland barley of physics, make the residual of beta-glucan organic solvent-free, cost is low, yield is high.
Summary of the invention
The method of the present invention's enriching beta glucan from highland barley comprises following processing step:
1. clean: impurity such as flat grain in the removal hullessbarley seed and stone, highland barley shell;
2. eluriate: the hullessbarley seed water is eluriated, removed in the highland barley impurity such as miscellaneous silt;
3. dry: the hullessbarley seed after eluriating is carried out drying, and moisture content＜8% of control hullessbarley seed;
4. grind: by fluffing machine highland barley wheat bran is separated with highland barley wheat face dried hullessbarley seed, collect highland barley wheat bran;
5. enzyme goes out: highland barley wheat bran was toasted 0.1～10 hour at 80～300 ℃;
6. micronizing: isolated highland barley wheat bran is carried out micronizing, make the granularity of highland barley bran micropowder be not more than 75 μ m;
7. expanded: as in the highland barley bran micropowder, to add the water of highland barley bran micropowder weight 5～40%, after mixing, under 95～250 ℃ of temperature, pressure 0.1～5MPa, carry out expanded; Or adopt screw extrusion press and mould to carry out puffing;
8. pulverize: it is 90～150 μ m that the highland barley wheat bran after expanded is crushed to granularity;
9. packing: airtight package or vacuum-packed or charge into the rare gas element packing.
After measured, in the highland barley bran micropowder that the inventive method is produced, the content of beta-glucan can reach about 35%, purifies as further processing, can optimize high-content beta-glucan preparation technology.
Because the present invention adopts beta-glucan in the mode enrichment highland barley of physics,, make in the beta-glucan extract of enrichment organic solvent-free residual without any the participation of solvent.
Simultaneously, make water-soluble beta-glucan and water-insoluble beta-glucan in the highland barley all obtain enrichment, avoided that the water-insoluble beta-glucan discards loss and the waste that causes in other extracting method with waste material owing to adopt physics mode.
In addition, enriching method technology of the present invention is simple, cost is low, and the yield height is a kind of effective beta-glucan enriching method.
Embodiment
Embodiment 1, from highland barley the method for enriching beta glucan:
1. clean: impurity such as flat grain in the removal hullessbarley seed and stone, highland barley shell;
2. eluriate: the hullessbarley seed water is eluriated, removed in the highland barley impurity such as miscellaneous silt;
3. dry: the hullessbarley seed after eluriating is carried out drying, and moisture content＜8% of control hullessbarley seed;
4. grind: by fluffing machine highland barley wheat bran is separated with highland barley wheat face dried hullessbarley seed, collect highland barley wheat bran;
5. enzyme goes out: highland barley wheat bran was toasted 0.1 hour at 300 ℃;
6. micronizing: isolated highland barley wheat bran is carried out micronizing, make the granularity of highland barley bran micropowder be not more than 75 μ m;
7. expanded: as in the highland barley bran micropowder, to add the water of highland barley bran micropowder weight 40%, after mixing, under 250 ℃ of temperature, pressure 0.1MPa, carry out expanded;
8. pulverize: it is 90～150 μ m that the highland barley wheat bran after expanded is crushed to granularity;
9. pack: airtight package.
Embodiment 2, from highland barley the method for enriching beta glucan:
1. clean: impurity such as flat grain in the removal hullessbarley seed and stone, highland barley shell;
2. eluriate: the hullessbarley seed water is eluriated, removed in the highland barley impurity such as miscellaneous silt;
3. dry: the hullessbarley seed after eluriating is carried out drying, and moisture content＜8% of control hullessbarley seed;
4. grind: by fluffing machine highland barley wheat bran is separated with highland barley wheat face dried hullessbarley seed, collect highland barley wheat bran;
5. enzyme goes out: highland barley wheat bran was toasted 0.5 hour at 200 ℃;
6. micronizing: isolated highland barley wheat bran is carried out micronizing, make the granularity of highland barley bran micropowder be not more than 75 μ m;
7. expanded: as in the highland barley bran micropowder, to add the water of highland barley bran micropowder weight 30%, after mixing, under 200 ℃ of temperature, pressure 0.5MPa, carry out expanded;
8. pulverize: it is 90～150 μ m that the highland barley wheat bran after expanded is crushed to granularity;
9. pack: vacuum packaging.
Embodiment 3, from highland barley the method for enriching beta glucan:
1. clean: impurity such as flat grain in the removal hullessbarley seed and stone, highland barley shell;
2. eluriate: the hullessbarley seed water is eluriated, removed in the highland barley impurity such as miscellaneous silt;
3. dry: the hullessbarley seed after eluriating is carried out drying, and moisture content＜8% of control hullessbarley seed;
4. grind: by fluffing machine highland barley wheat bran is separated with highland barley wheat face dried hullessbarley seed, collect highland barley wheat bran;
5. enzyme goes out: highland barley wheat bran was toasted 1 hour at 120 ℃;
6. micronizing: isolated highland barley wheat bran is carried out micronizing, make the granularity of highland barley bran micropowder be not more than 75 μ m;
7. expanded: as in the highland barley bran micropowder, to add the water of highland barley bran micropowder weight 20%, after mixing, under 150 ℃ of temperature, pressure 1MPa, carry out expanded;
8. pulverize: it is 90～150 μ m that the highland barley wheat bran after expanded is crushed to granularity;
9. pack: charge into the rare gas element packing.
Embodiment 4, from highland barley the method for enriching beta glucan:
1. clean: impurity such as flat grain in the removal hullessbarley seed and stone, highland barley shell;
2. eluriate: the hullessbarley seed water is eluriated, removed in the highland barley impurity such as miscellaneous silt;
3. dry: the hullessbarley seed after eluriating is carried out drying, and moisture content＜8% of control hullessbarley seed;
4. grind: by fluffing machine highland barley wheat bran is separated with highland barley wheat face dried hullessbarley seed, collect highland barley wheat bran;
5. enzyme goes out: highland barley wheat bran was toasted 10 hours at 80 ℃;
6. micronizing: isolated highland barley wheat bran is carried out micronizing, make the granularity of highland barley bran micropowder be not more than 75 μ m;
7. expanded: as in the highland barley bran micropowder, to add the water of highland barley bran micropowder weight 20%, after mixing, adopt screw extrusion press and mould to carry out puffing;
8. pulverize: it is 90～150 μ m that the highland barley wheat bran after expanded is crushed to granularity;
9. pack: vacuum packaging.
Embodiment 5, from highland barley the method for enriching beta glucan:
1. clean: impurity such as flat grain in the removal hullessbarley seed and stone, highland barley shell;
2. eluriate: the hullessbarley seed water is eluriated, removed in the highland barley impurity such as miscellaneous silt;
3. dry: the hullessbarley seed after eluriating is carried out drying, and moisture content＜8% of control hullessbarley seed;
4. grind: by fluffing machine highland barley wheat bran is separated with highland barley wheat face dried hullessbarley seed, collect highland barley wheat bran;
5. enzyme goes out: highland barley wheat bran was toasted 1 hour at 120 ℃;
6. micronizing: isolated highland barley wheat bran is carried out micronizing, make the granularity of highland barley bran micropowder be not more than 75 μ m;
7. expanded: as in the highland barley bran micropowder, to add the water of highland barley bran micropowder weight 5～40%, after mixing, adopt screw extrusion press and mould to carry out puffing;
8. pulverize: it is 90～150 μ m that the highland barley wheat bran after expanded is crushed to granularity;
9. pack: charge into the rare gas element packing.
Claims (1)
1, a kind of method of extracting beta-glucan from highland barley comprises following processing step:
1. clean: flat grain in the removal hullessbarley seed and stone, highland barley shell impurity;
2. eluriate: the hullessbarley seed water is eluriated, removed miscellaneous silt impurity in the highland barley;
3. dry: the hullessbarley seed after eluriating is carried out drying, and moisture content＜8% of control hullessbarley seed;
4. grind: by fluffing machine highland barley wheat bran is separated with highland barley wheat face dried hullessbarley seed, collect highland barley wheat bran;
5. enzyme goes out: highland barley wheat bran was toasted 0.1～10 hour at 80～300 ℃;
6. micronizing: isolated highland barley wheat bran is carried out micronizing, make the granularity of highland barley bran micropowder be not more than 75 μ m;
7. expanded: as in the highland barley bran micropowder, to add the water of highland barley bran micropowder weight 5～40%, after mixing, under 95～250 ℃ of temperature, pressure 0.1～5MPa, carry out expanded; Or adopt screw extrusion press and mould to carry out puffing;
8. pulverize: it is 90～150 μ m that the highland barley wheat bran after expanded is crushed to granularity;
9. packing: airtight package or vacuum-packed or charge into the rare gas element packing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100430965A CN100434442C (en) | 2006-06-26 | 2006-06-26 | Method for enriching beta glucan from highland barley |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100430965A CN100434442C (en) | 2006-06-26 | 2006-06-26 | Method for enriching beta glucan from highland barley |
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| CN1872884A CN1872884A (en) | 2006-12-06 |
| CN100434442C true CN100434442C (en) | 2008-11-19 |
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101120776B (en) * | 2007-06-23 | 2011-04-06 | 西藏天麦力健康品有限公司 | Method for extracting beta-glucan from cereal bran using membrane separation technology |
| CN101433282B (en) * | 2007-11-14 | 2013-04-03 | 厦门伯赛基因转录技术有限公司 | Method for enriching and extracting beta-dextran from cereal |
| CN101283752B (en) * | 2008-05-09 | 2011-03-16 | 甘肃奇正实业集团有限公司 | Processing method of puffing highland barley rice slice |
| CN101921345A (en) * | 2010-08-13 | 2010-12-22 | 南昌大学 | Method for extracting polysaccharide and flavone from bamboo leaves |
| CN102432693B (en) * | 2011-12-12 | 2016-01-13 | 北京工商大学 | A kind of method extracting beta-glucosan from oat bran |
| CN103330140A (en) * | 2013-06-17 | 2013-10-02 | 西藏现代农业有限公司 | Highland barley oatmeal as well as preparation method thereof |
| CN104012877B (en) * | 2014-06-25 | 2018-09-07 | 叶炳年 | A kind of preparation method of unstrained highland barley spirit |
| CN104286687B (en) * | 2014-10-11 | 2017-07-21 | 天津北洋百川生物技术有限公司 | A kind of preparation method rich in beta glucan malt extract |
| CN105053802A (en) * | 2015-08-10 | 2015-11-18 | 康巴情人食品有限责任公司 | Highland barley instant powder and preparation method thereof |
| CN108208587A (en) * | 2016-12-09 | 2018-06-29 | 浙江大学自贡创新中心 | A kind of processing method of grain dust of enriching beta glucan and its equipment of use |
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| US5512287A (en) * | 1994-05-12 | 1996-04-30 | Centennial Foods, Inc. | Production of β-glucan and β-glucan product |
| CN1597702A (en) * | 2004-08-13 | 2005-03-23 | 西藏青稞研究与发展中心 | Method of extrating bela dextran from highland barley |
| CN1597701A (en) * | 2004-08-13 | 2005-03-23 | 西藏青稞研究与发展中心 | Beta dextran preparation extracted from highland barley and its composition |
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2006
- 2006-06-26 CN CNB2006100430965A patent/CN100434442C/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5512287A (en) * | 1994-05-12 | 1996-04-30 | Centennial Foods, Inc. | Production of β-glucan and β-glucan product |
| CN1597702A (en) * | 2004-08-13 | 2005-03-23 | 西藏青稞研究与发展中心 | Method of extrating bela dextran from highland barley |
| CN1597701A (en) * | 2004-08-13 | 2005-03-23 | 西藏青稞研究与发展中心 | Beta dextran preparation extracted from highland barley and its composition |
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| Title |
|---|
| 西藏青稞中beta-葡聚糖提取的研究. 连喜军等.天津农学院学报，第12卷第4期. 2005 |
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Owner name: TIBETAN QIZHENG RAW TYPE HEALTH PRODUCTS CO., LTD Free format text: FORMER OWNER: GANSU QIZHENG TIBETAN MEDICINE CO., LTD. Effective date: 20091030 |
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Effective date of registration: 20091030 Address after: No. 808 Chang Zhang Tan, Chengguan District (Lanzhou hi tech Development Zone), Gansu Patentee after: Tibet Qizheng Original Ecological Health Products Co., Ltd. Address before: Taking Yuzhong County of Gansu Province, Lanzhou City Road Patentee before: Gansu Qizheng Tibetan Medicine Co., Ltd. |
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| C56 | Change in the name or address of the patentee |
Owner name: TIBET TIANMAILI HEALTH PRODUCTS CO., LTD. Free format text: FORMER NAME: TIBETAN QIZHENG RAW TYPE HEALTH PRODUCTS CO., LTD |
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| CP01 | Change in the name or title of a patent holder |
Address after: 730010, No. 808 Chang Zhang Tan, Chengguan District (Lanzhou hi tech Development Zone), Gansu, China Patentee after: Tibet Tianmaili Health Food Co., Ltd. Address before: 730010, No. 808 Chang Zhang Tan, Chengguan District (Lanzhou hi tech Development Zone), Gansu, China Patentee before: Tibet Qizheng Original Ecological Health Products Co., Ltd. |
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| CP03 | Change of name, title or address |
Address after: 851400 No. 11, Linqionggang Road, Lhasa Economic and Technological Development Zone, Tibet Autonomous Region Patentee after: Tibet Qizheng Highland Barley Health Technology Co., Ltd. Address before: 730010, No. 808 Chang Zhang Tan, Chengguan District (Lanzhou hi tech Development Zone), Gansu, China Patentee before: Tibet Tianmaili Health Food Co., Ltd. |
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| CP03 | Change of name, title or address |