CN108851041A - A kind of composition and preparation method thereof containing dendrobium leaf with reduction postprandial blood sugar effect - Google Patents
A kind of composition and preparation method thereof containing dendrobium leaf with reduction postprandial blood sugar effect Download PDFInfo
- Publication number
- CN108851041A CN108851041A CN201810516340.8A CN201810516340A CN108851041A CN 108851041 A CN108851041 A CN 108851041A CN 201810516340 A CN201810516340 A CN 201810516340A CN 108851041 A CN108851041 A CN 108851041A
- Authority
- CN
- China
- Prior art keywords
- freeze
- dried powder
- dendrobium leaf
- blood sugar
- drying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001523681 Dendrobium Species 0.000 title claims abstract description 60
- 239000008280 blood Substances 0.000 title claims abstract description 57
- 210000004369 blood Anatomy 0.000 title claims abstract description 57
- 235000000346 sugar Nutrition 0.000 title claims abstract description 40
- 230000000291 postprandial effect Effects 0.000 title claims abstract description 34
- 239000000203 mixture Substances 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 230000000694 effects Effects 0.000 title claims abstract description 21
- 230000009467 reduction Effects 0.000 title description 6
- 238000004108 freeze drying Methods 0.000 claims abstract description 34
- 239000000843 powder Substances 0.000 claims abstract description 34
- 240000000588 Hericium erinaceus Species 0.000 claims abstract description 30
- 235000007328 Hericium erinaceus Nutrition 0.000 claims abstract description 30
- 230000007062 hydrolysis Effects 0.000 claims abstract description 30
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 30
- 108090000790 Enzymes Proteins 0.000 claims abstract description 28
- 102000004190 Enzymes Human genes 0.000 claims abstract description 28
- 229940088598 enzyme Drugs 0.000 claims abstract description 28
- 238000000034 method Methods 0.000 claims abstract description 28
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 19
- 108010059892 Cellulase Proteins 0.000 claims abstract description 18
- 239000002253 acid Substances 0.000 claims abstract description 18
- 241001092040 Crataegus Species 0.000 claims abstract description 17
- 235000009917 Crataegus X brevipes Nutrition 0.000 claims abstract description 17
- 235000013204 Crataegus X haemacarpa Nutrition 0.000 claims abstract description 17
- 235000009685 Crataegus X maligna Nutrition 0.000 claims abstract description 17
- 235000009444 Crataegus X rubrocarnea Nutrition 0.000 claims abstract description 17
- 235000009486 Crataegus bullatus Nutrition 0.000 claims abstract description 17
- 235000017181 Crataegus chrysocarpa Nutrition 0.000 claims abstract description 17
- 235000009682 Crataegus limnophila Nutrition 0.000 claims abstract description 17
- 235000004423 Crataegus monogyna Nutrition 0.000 claims abstract description 17
- 235000002313 Crataegus paludosa Nutrition 0.000 claims abstract description 17
- 235000009840 Crataegus x incaedua Nutrition 0.000 claims abstract description 17
- 229940106157 cellulase Drugs 0.000 claims abstract description 16
- 230000001603 reducing effect Effects 0.000 claims abstract description 15
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 239000007787 solid Substances 0.000 claims description 19
- 230000008569 process Effects 0.000 claims description 17
- 238000004140 cleaning Methods 0.000 claims description 15
- 238000001035 drying Methods 0.000 claims description 15
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 14
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 14
- 238000007710 freezing Methods 0.000 claims description 11
- 230000008014 freezing Effects 0.000 claims description 11
- 235000003599 food sweetener Nutrition 0.000 claims description 9
- 239000003765 sweetening agent Substances 0.000 claims description 9
- 239000002552 dosage form Substances 0.000 claims description 8
- 240000002853 Nelumbo nucifera Species 0.000 claims description 6
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims description 6
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 239000008187 granular material Substances 0.000 claims description 6
- 229940098465 tincture Drugs 0.000 claims description 6
- 238000005520 cutting process Methods 0.000 claims description 5
- 239000006187 pill Substances 0.000 claims description 5
- 238000005360 mashing Methods 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 3
- 235000007106 Crataegus suborbiculata Nutrition 0.000 claims description 3
- 241000073432 Crataegus suborbiculata Species 0.000 claims description 3
- 235000013202 a hawthorn Nutrition 0.000 claims description 3
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 3
- 239000001630 malic acid Substances 0.000 claims description 3
- 235000011090 malic acid Nutrition 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 239000003826 tablet Substances 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 235000005979 Citrus limon Nutrition 0.000 claims 2
- 244000131522 Citrus pyriformis Species 0.000 claims 2
- 238000003756 stirring Methods 0.000 claims 1
- 239000001913 cellulose Substances 0.000 abstract description 13
- 229920002678 cellulose Polymers 0.000 abstract description 13
- 235000013305 food Nutrition 0.000 abstract description 12
- 239000000463 material Substances 0.000 abstract description 8
- 239000001814 pectin Substances 0.000 abstract description 8
- 235000010987 pectin Nutrition 0.000 abstract description 8
- 229920001277 pectin Polymers 0.000 abstract description 8
- 239000003814 drug Substances 0.000 abstract description 7
- 229940079593 drug Drugs 0.000 abstract description 6
- 240000004638 Dendrobium nobile Species 0.000 abstract description 5
- 239000000047 product Substances 0.000 description 18
- 150000004676 glycans Chemical class 0.000 description 17
- 229920001282 polysaccharide Polymers 0.000 description 16
- 239000005017 polysaccharide Substances 0.000 description 16
- 239000008103 glucose Substances 0.000 description 15
- 239000000126 substance Substances 0.000 description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 13
- 230000003078 antioxidant effect Effects 0.000 description 8
- 239000003963 antioxidant agent Substances 0.000 description 7
- 235000006708 antioxidants Nutrition 0.000 description 7
- 229930003935 flavonoid Natural products 0.000 description 7
- 150000002215 flavonoids Chemical class 0.000 description 7
- 235000017173 flavonoids Nutrition 0.000 description 7
- 238000012545 processing Methods 0.000 description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 230000002218 hypoglycaemic effect Effects 0.000 description 6
- 230000000144 pharmacologic effect Effects 0.000 description 6
- 238000006731 degradation reaction Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 229920001542 oligosaccharide Polymers 0.000 description 5
- 150000002482 oligosaccharides Chemical class 0.000 description 5
- 150000003648 triterpenes Chemical class 0.000 description 5
- 241000026010 Dendrobium candidum Species 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 230000015556 catabolic process Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- 239000003755 preservative agent Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 240000007711 Peperomia pellucida Species 0.000 description 3
- 238000010241 blood sampling Methods 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 230000037213 diet Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000019441 ethanol Nutrition 0.000 description 3
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000002335 preservative effect Effects 0.000 description 3
- 241000228245 Aspergillus niger Species 0.000 description 2
- 235000017159 Crataegus pinnatifida Nutrition 0.000 description 2
- 241000657480 Crataegus pinnatifida Species 0.000 description 2
- -1 DPPH free radical Chemical class 0.000 description 2
- 241001076416 Dendrobium tosaense Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000123222 Hericium Species 0.000 description 2
- 241000209477 Nymphaeaceae Species 0.000 description 2
- 108010029182 Pectin lyase Proteins 0.000 description 2
- 235000004789 Rosa xanthina Nutrition 0.000 description 2
- 241000220222 Rosaceae Species 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930013930 alkaloid Natural products 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 230000003110 anti-inflammatory effect Effects 0.000 description 2
- 102000006995 beta-Glucosidase Human genes 0.000 description 2
- 108010047754 beta-Glucosidase Proteins 0.000 description 2
- 230000036772 blood pressure Effects 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000001684 chronic effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 201000001421 hyperglycemia Diseases 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 150000002772 monosaccharides Chemical class 0.000 description 2
- 230000002107 myocardial effect Effects 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 108020004410 pectinesterase Proteins 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 150000003505 terpenes Chemical class 0.000 description 2
- 235000007586 terpenes Nutrition 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 108010084185 Cellulases Proteins 0.000 description 1
- 102000005575 Cellulases Human genes 0.000 description 1
- 108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 description 1
- 208000028698 Cognitive impairment Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- AEMOLEFTQBMNLQ-YMDCURPLSA-N D-galactopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-YMDCURPLSA-N 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
- 108010088406 Glucagon-Like Peptides Proteins 0.000 description 1
- 229940122069 Glycosidase inhibitor Drugs 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 208000013016 Hypoglycemia Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 206010054805 Macroangiopathy Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241000606860 Pasteurella Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 241000208966 Polygala Species 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 208000017442 Retinal disease Diseases 0.000 description 1
- 206010038923 Retinopathy Diseases 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 206010049418 Sudden Cardiac Death Diseases 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 208000010877 cognitive disease Diseases 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 230000008694 endothelial dysfunction Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 230000002431 foraging effect Effects 0.000 description 1
- 230000001408 fungistatic effect Effects 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000003316 glycosidase inhibitor Substances 0.000 description 1
- 235000021552 granulated sugar Nutrition 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 239000004026 insulin derivative Substances 0.000 description 1
- 230000003914 insulin secretion Effects 0.000 description 1
- 239000012263 liquid product Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000004224 protection Effects 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 238000005059 solid analysis Methods 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000002936 tranquilizing effect Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 238000004260 weight control Methods 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/09—Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L25/00—Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof
- A23L25/30—Mashed or comminuted products, e.g. pulp, pastes, meal, powders; Products made therefrom, e.g. blocks, flakes, snacks; Liquid or semi-liquid products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/10—General methods of cooking foods, e.g. by roasting or frying
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention provides a kind of with the composition containing dendrobium leaf for reducing postprandial blood sugar effect, is made of the raw material of following parts by weight：1-3 parts of the freeze-dried powder of dendrobium leaf hydrolyzate；1-3 parts of the freeze-dried powder of Hericium erinaceus hydrolyzate；1-3 parts of embryo nelumbinis freeze-dried powder；1-3 parts of hawthorn freeze-dried powder；1-3 parts of sugar-acids mouthfeel instrumentality.The present invention also provides the preparation methods of above-mentioned composition, cellulase and pectin enzyme hydrolysis and freeze-drying method including dendrobium leaf；The cellulose hydrolyzation and freeze-drying method of Hericium erinaceus；Embryo nelumbinis and hawthorn freeze-dried powder；Above-mentioned each material is proportionally mixed, composition is prepared into.Postprandial blood sugar can be effectively reduced in product of the present invention, and each material is dual-purpose of drug and food material, can be taken for a long time, safe and in good taste.Meanwhile the present invention has expanded the utilization scope of dendrobium leaf, sufficiently develops and uses other positions other than stem, improves the utilization rate of dendrobium nobile plant.
Description
Technical field
The invention belongs to field of food, are related to a kind of health food, specifically a kind of that there is reduction postprandial blood sugar to make
Composition and preparation method thereof containing dendrobium leaf.
Background technique
With closing on for aging society, chronic epidemic disease becomes main epidemic disease.Postprandial hyperglycemia is the only of macrovascular diseases
Vertical risk factor.Postprandial hyperglycemia and retinopathy risk increase, Carotid Intima-media Thickness increases, myocardial blood volume and
Myocardial blood flow decline, risk of cancer are increased and the elderly's cognitive impairment is related, can also cause oxidative stress, inflammation and endothelium
Dysfunction.Epidemiological study the result shows that：Reduce the generation that postprandial blood sugar can be effectively reduced sudden cardiac death.
There are many modes of control blood glucose：Low suger diet is beneficial to control postprandial blood sugar；Some drugs are to the postprandial blood of control
It is sugared preferable；Diet intervention, physical exertion and body weight control are still the foundation stone of blood glucose control.Regulation postprandial blood sugar drug include
Glycosidase inhibitor, Drugs Promoting Insulin Secretion, insulin and its insulin analog, glucagon-like peptide etc..Drug is controlled
It is higher to treat price, and takes for a long time with certain toxic side effect (such as hypoglycemia, weight gain and gastrointestinal reaction), makees
Method to control postprandial blood sugar throughout one's life has certain drawbacks.
Contemporary society, cardiovascular and cerebrovascular epidemic disease have become the major chronic epidemic disease of person in middle and old age.Safety on domestic market
The effective health care product for adjusting postprandial blood sugar is less, therefore the health product exploitation of the reduction postprandial blood sugar based on natural food,
Have great importance.
Summary of the invention
For above-mentioned technical problem in the prior art, the present invention provides a kind of containing with reduction postprandial blood sugar effect
There are the composition and preparation method thereof of dendrobium leaf, this combination containing dendrobium leaf with reduction postprandial blood sugar effect
Object and preparation method thereof, which will solve food in the prior art, reduces the ineffective technical problem of postprandial blood sugar.
The present invention provides a kind of with the composition containing dendrobium leaf for reducing postprandial blood sugar effect, by following parts by weight
Several raw materials are prepared：
Further, the dosage form of composition can be pulvis, granule, pill, tablet, tincture or aqua.
Further, the sugar-acids mouthfeel Auto-regulator is made of sweetener and acid, sweetener and acid
Mass ratio be 50-800：1, the sweetener is the combination of any one or two kinds in sorbierite or protein sugar；Institute
The acid stated is the combination of citric acid, any one or two kinds in malic acid.
The present invention also provides a kind of above-mentioned systems with the composition containing dendrobium leaf for reducing postprandial blood sugar effect
Preparation Method includes the following steps：
1) a preparation dendrobium leaf hydrolyzate and the step of freeze-dried powder, by the sorting of fresh dendrobium leaf, cleaning and drained
Afterwards, according to mass ratio 1:1 adds water mashing, successively hydrolyzes dendrobium leaf using cellulase and pectase and prepares its homogenate, fiber
The enzymatic hydrolysis condition of plain enzyme is：Solid-liquid ratio is 1g：60-100ml, enzyme concentration are the 1.0-1.5% of feed liquid gross mass, and hydrolysis temperature is
40-60 DEG C, enzymolysis time 2-8h, enzymatic hydrolysis pH is 5.0-9.0；The enzymatic hydrolysis condition of pectase is：Solid-liquid ratio is 1g：60-
100ml；Enzyme concentration is the 0.3-0.8% of feed liquid gross mass, hydrolysis temperature is 40-60 DEG C, enzymolysis time 1-3h；Digesting pH is
3.0-7.0；5~15min is boiled after hydrolysis；Cooling is placed on -80 DEG C of refrigerator freezings for 24 hours, then carries out freeze-drying process, sets
- 60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h；Solid after freeze-drying is crushed with pulverizer, is collected after crossing 60 meshes
It is spare；
2) a preparation Hericium erinaceus hydrolyzate and the step of freeze-dried powder；After dry Hericium erinaceus cleaning, crushes, cross 100 mesh
Classifying screen, screenings add water and cellulase to be hydrolyzed, and enzyme hydrolysis treatment conditions are：Material-water ratio 1g：40-80ml adds
Enzyme amount is the 0.6-1.5% for expecting water gross mass, and enzymolysis time 3-9h, hydrolysis temperature is 30-50 DEG C, pH value of solution 6.0-8.0,
5~15min is boiled after hydrolysis；Cooling is placed on -80 DEG C of refrigerator freezings for 24 hours, then carries out freeze-drying process, sets cold-trap temperature
- 60 DEG C, vacuum degree 1MPa, drying time 48h of degree；Solid after freeze-drying is crushed with pulverizer, is collected after 60 meshes excessively spare；
3) preparation step of the freeze-dried powder of an embryo nelumbinis：Embryo nelumbinis is placed in -80 DEG C of refrigerator freezings by sorting, cleaning
For 24 hours, then freeze-drying process is carried out；- 60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h of setting；Solid after freeze-drying
It is crushed, is collected after 60 meshes excessively spare with pulverizer.
4) preparation step of the freeze-dried powder of a hawthorn：Hawthorn is sorted, cleaning, is enucleated, after cutting, is placed in -80 DEG C of ice
Case freezes for 24 hours, then carries out freeze-drying process, sets -60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h；After freeze-drying
Solid crushed with pulverizer, cross 60 meshes after collect it is spare；
5) preparation step of a composition product：By the freeze-drying of the freeze-dried powder of dendrobium leaf hydrolyzate, Hericium erinaceus hydrolyzate
Powder, embryo nelumbinis freeze-dried powder, hawthorn freeze-dried powder and sugar-acids mouthfeel instrumentality are mixed according to mass fraction, are stirred evenly, and are made each
Kind dosage form has the composition containing dendrobium leaf for reducing postprandial blood sugar effect, and the dosage form includes pulvis, granule, ball
Agent, tincture or aqua.
The purpose of the present invention is developing a kind of composition for adjusting postprandial blood sugar, the Component Source in the composition is in food
Object has effects that inhibit postprandial blood sugar rapid increase, does not have illeffects to human body, and sensory properties is easy to be accepted.
To achieve the goals above, for the present invention by pharmacological techniques, biotechnology and foods processing technique develop one
Kind adjusts the food-based composition of postprandial blood sugar.
The present invention also provides the preparation methods of above-mentioned composition, the cellulase and pectin enzyme hydrolysis including dendrobium leaf and
Freeze-drying method；The cellulase and pectin enzyme hydrolyzate and freeze-drying method of Hericium erinaceus；Embryo nelumbinis and hawthorn freeze-dried powder
Preparation method and final composition product preparation step, product of the present invention is proportionally to mix above-mentioned each material, system
For at composition.
Dendrobium candidum (Dendrobiumofficinale Kimura et Migo) mainly has enhancing immune, antifatigue, anti-
The pharmacological actions such as oxidation, hypoglycemic, blood pressure lowering.Dendrobium leaf is the leaf of dendrobium candidum plant.Dendrobium nobile leaf is often used as food
With drug processing waste and abandon.Research shows that：Dendrobium candidum stem from leaf respectively contain higher different effective components, always
Polysaccharide focuses primarily upon stem, and total alkaloid and general flavone focus primarily upon leaf.Stem and Ye Jun can be used as edible medicinal resource and filled
Divide and utilizes.In antioxidant activity experiment, remove DPPH free radical the results show that leaf portion polysaccharide is substantially better than stem's polysaccharide,
Show its anti-oxidant application value with higher.But the hypoglycemic effect of dendrobium nobile leaf has not been reported.
Hericium erinaceus (Hericium ernaceus) is a kind of fungi, and hericium erinaceum polysaccharide is the main active of Hericium erinaceus.
Modern medicine and pharmacological many researchs have shown that hericium erinaceum polysaccharide has and improve immunity, antitumor, anti-aging, reducing blood lipid etc.
Different physiological roles.Polysaccharide and oligosaccharides in Hericium erinaceus have hypoglycemic effect.Terpene and phenols in Hericium erinaceus is to golden yellow
Staphylococcus, Escherichia coli, bacillus subtilis all have certain fungistatic effect.
Lotus Plumule (Nelumbinis plumula) is drying spire and radicle in the mature seed of nymphaeaceae plant lotus,
With clearing away the heart fire and tranquillizing, relieving cough and reducing sputum, hemostasis is anti-inflammatory and other effects.Chemical component in Lotus Plumule is mainly alkaloids, additionally
There are the ingredients such as flavonoids, polysaccharide and volatile oil, there are the multiple pharmacological effects such as reducing blood lipid, anti-oxidant, anti-inflammatory.Embryo nelumbinis water mentions
Object has in various degree escherichia coli, streptococcus, staphylococcus aureus, salmonella typhimurium, Pasteurella etc.
Bacteriostasis.Therefore by the bacteriostatic activity of addition embryo nelumbinis, microbial growth in product can be prevented, preservative is played
Effect.Therefore, embryo nelumbinis can not only provide nutriment, can also play the role of antioxidant and preservative, extend and protect
The matter phase avoids the use of exogenous additive.
Hawthorn (Crataegus pinnatifida) is the fruit of rosaceae may.It is rich in hawthorn
Flavonoids, terpene and vitamin substances have the function of blood pressure lowering, anticoagulation, anti-oxidant and protection endothelial cell.
This patent cooperates dendrobium leaf, Hericium erinaceus, embryo nelumbinis and hawthorn, adds in conjunction with pharmacological experiment technology, biology
Work technology and food processing means carry out product formula optimization, product processing and functional verification to it, and developing dendrobium leaf is using newly
The utilization rate of dendrobium nobile is improved in field, has great importance to the scientific and reasonable diet of the special populations such as dysglycemia.
The raw materials such as dendrobium leaf, Hericium erinaceus, embryo nelumbinis and hawthorn, traditional processing mode are that water mentions or alcohol extracting, again to extract
It is further processed.Since the water solubility of polysaccharide and triterpenes, Flavonoid substances has very big difference, water is mentioned or alcohol extracting can
Lead to a large amount of losses of other non-solubility ingredients.Therefore, cellulose hydrolyzation is used in this patent, on the one hand improves polysaccharide
Bioavilability, another aspect cellulose hydrolysis after triterpene substance can be released, finally collect hydrolysis after it is complete
Portion's liquid.Due to not extracting a certain substance individually, the loss of other function ingredient in extraction process is avoided.To the maximum extent
Remain the content of polysaccharide and triterpenes, Flavonoid substances.
The dendrobium candidum (Dendrobiumofficinale Kimura et Migo) of dendrobium leaf involved in the present invention it is green
Color blade.Dendrobium leaf hydrolyzate involved in the present invention is dendrobium leaf by cellulase and pectin enzyme hydrolysis and filtered liquid
State product, containing the various polysaccharide materials and the nutriments such as mineral element, protein in dendrobium leaf in solution, and meanwhile it is solvable
Property carbohydrate (oligosaccharide such as monosaccharide, oligosaccharides, dextrin and soluble polysaccharide) content obviously increase than not hydrolyzing blade, be mostly derived from stone
The degradation of cellulose and pectin substance in dry measure used in former times leaf.Cellulase involved in the present invention is that catalytic degradation cellulose generates glucose
It is main with the general name of one group of enzyme of low polymerization degree fiber, including endoglucanase, 1,4-BETA-D-glucancellobio-hydrolase and cellobiase 3
Component (SIGMA reagent, C1184 derive from Aspergillus niger).Pectase involved in the present invention is mixture,
CAS:9032-75-1, Sigma Products, 3 main components of pectase are pectinesterase (EC 3.1.1.11), poly- gala
Uronic acid enzyme (EC3.2.1.15), pectin lyase (EC 4.2.2.10), can make hydrolyzed pectin sucrose and galacturonic acid.
It is crushed after dendrobium leaf cleaning, weighs and wiring solution-forming carries out enzyme hydrolysis processing.
The Hericium erinaceus (Hericium ernaceus) that the present invention uses is the fructification after a kind of fungi is dry.The present invention
Hericium erinaceus hydrolyzate to be Hericium erinaceus contain in Hericium erinaceus by cellulose hydrolyzation and filtered liquid product, solution
The nutritional ingredients such as polysaccharide, Flavonoid substances, minerals, but soluble saccharide (monosaccharide, oligosaccharides, oligosaccharide and soluble polysaccharide) contains
Amount does not hydrolyze Hericium erinaceus than original and obviously increases, the cellulose degraded being mostly derived from fruit.Cellulose involved in the present invention
Enzyme is the general name for one group of enzyme that catalytic degradation cellulose generates glucose and low polymerization degree fiber, including endoglucanase, Portugal
3 main components of glycan excision enzyme and cellobiase (SIGMA reagent, C1184 derive from Aspergillus niger).Claim
After taking quantitative Hericium erinaceus slurries, wiring solution-forming carries out cellulase hydrolysis.
The Lotus Plumule (Nelumbinis plumula) that the present invention uses is dry in the mature seed of nymphaeaceae plant lotus
Dry spire and radicle.Embryo nelumbinis is placed in -80 DEG C of refrigerator freezings for 24 hours, then carry out freeze-drying process by sorting, cleaning.Setting
- 60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h.；Solid after freeze-drying is crushed with pulverizer, crosses 60 meshes.
The hawthorn (Crataegus pinnatifida) that the present invention uses is the fruit of rosaceae may.Hawthorn
After sorted, cleaning, stoning, cutting, it is placed in -80 DEG C of refrigerator freezings for 24 hours, then carry out freeze-drying process.Set condenser temperature-
60 DEG C, vacuum degree 1MPa, drying time 48h.；Solid after freeze-drying is crushed with pulverizer, crosses 60 meshes.
Object of the present invention is to carry out compatibility by dendrobium leaf, Hericium erinaceus, embryo nelumbinis and hawthorn, using pharmacological outcomes as matching
The Testing index of Fang Youhua develops a kind of product for reducing postprandial blood sugar, reaches nutrition and functional complementation, and improve digestion
And absorptivity, and solid form facilitates storage and transportation or the addition in other food as primary products.
Present invention employs the combination of the freeze-dried powder of dendrobium leaf, the cellulase of Hericium erinaceus and pectin protease hydrolysate, nutrition
And have complementary functions, on the one hand enhance trophism and functionality, enhance hypoglycemic healthcare function, improve more in product
The digestibility and absorptivity of carbohydrate and cellulose substances；Free state polysaccharide in finished product, triterpene substance, Huang are improved simultaneously
Letones and other function content of material, enhance functional components, and the Flavonoid substances in embryo nelumbinis and hawthorn have
Antibacterial antioxidation can extend the shelf-life of final products in the case where not outer adding preservative agent and antioxidant.It reduces
The usage amount of antioxidant and preservative in product.
The composition sour and sweet palatability of method preparation of the invention, no bitter taste, color yellowish white is direct-edible, or
Beverage is diluted to liquid (water or ethyl alcohol) to eat, and be can also be used as ingredient and is added in other food.
The present invention is compared with prior art, and technological progress is significant.Present invention employs cellulases and pectase
Hydrolysis, on the one hand improves the bioavilability of insoluble polysaccharide in dendrobium leaf and Hericium erinaceus；On the other hand, cellulose or pectin
Triterpene substance, Flavonoid substances and the phenolic substances etc. in dendrobium leaf and Hericium erinaceus can be released after hydrolysis, not only be had
Effect of functional complementation also has and cooperates with anti-oxidant and anti-aging effects.The technology of the present invention is simple, noresidue, pollution-free, no
It is limited by room and time, be there is no adverse effect to processing space facility and people.
Detailed description of the invention
Fig. 1 shows influence of the dendrobium leaf hydrolyzate freeze-dried powder to animal postprandial blood sugar.
Specific embodiment
Embodiment 1:
The present invention provides a kind of with the composition containing dendrobium leaf for reducing postprandial blood sugar effect, by following parts by weight
Several raw materials are prepared：
Further, dosage form can be pulvis, granule, pill, tablet, tincture or aqua.
Further, the sugar-acids mouthfeel Auto-regulator is made of sweetener and acid, sweetener and acid
Mass ratio be 50-800：1, the sweetener is the combination of sorbierite, any one or two kinds in protein sugar；Described
Acid is the combination of any one or two kinds in citric acid or malic acid.
The present invention also provides a kind of above-mentioned systems with the composition containing dendrobium leaf for reducing postprandial blood sugar effect
Preparation Method includes the following steps：
1) a preparation dendrobium leaf hydrolyzate and the step of freeze-dried powder, by the sorting of fresh dendrobium leaf, cleaning and drained
Afterwards, according to mass ratio 1:1 adds water mashing, successively hydrolyzes dendrobium leaf using cellulase and pectase and prepares its homogenate, fiber
The enzymatic hydrolysis condition of plain enzyme is：Solid-liquid ratio is 1g：60-100ml, enzyme concentration are the 1.0-1.5% of feed liquid gross mass, and hydrolysis temperature is
40-60 DEG C, enzymolysis time 2-8h, enzymatic hydrolysis pH is 5.0-9.0；The enzymatic hydrolysis condition of pectase is：Solid-liquid ratio is 1g：60-
100ml；Enzyme concentration is the 0.3-0.8% of feed liquid gross mass, hydrolysis temperature is 40-60 DEG C, enzymolysis time 1-3h；Digesting pH is
3.0-7.0；5~15min is boiled after hydrolysis；Cooling is placed on -80 DEG C of refrigerator freezings for 24 hours, then carries out freeze-drying process, sets
- 60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h；Solid after freeze-drying is crushed with pulverizer, is collected after crossing 60 meshes
It is spare；
2) a preparation Hericium erinaceus hydrolyzate and the step of freeze-dried powder；After dry Hericium erinaceus cleaning, crushes, cross 100 mesh
Classifying screen, screenings add water and cellulase to be hydrolyzed, and enzyme hydrolysis treatment conditions are：Material-water ratio 1g：40-80ml adds
Enzyme amount is the 0.6-1.5% for expecting water gross mass, and enzymolysis time 3-9h, hydrolysis temperature is 30-50 DEG C, pH value of solution 6.0-8.0,
5~15min is boiled after hydrolysis；Cooling is placed on -80 DEG C of refrigerator freezings for 24 hours, then carries out freeze-drying process, sets cold-trap temperature
- 60 DEG C, vacuum degree 1MPa, drying time 48h of degree；Solid after freeze-drying is crushed with pulverizer, is collected after 60 meshes excessively spare；
3) preparation step of the freeze-dried powder of an embryo nelumbinis：Embryo nelumbinis is placed in -80 DEG C of refrigerator freezings by sorting, cleaning
For 24 hours, then freeze-drying process is carried out；- 60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h of setting；Solid after freeze-drying
It is crushed, is collected after 60 meshes excessively spare with pulverizer.
4) preparation step of the freeze-dried powder of a hawthorn：Hawthorn is sorted, cleaning, is enucleated, after cutting, is placed in -80 DEG C of ice
Case freezes for 24 hours, then carries out freeze-drying process, sets -60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h；After freeze-drying
Solid crushed with pulverizer, cross 60 meshes after collect it is spare；
5) preparation step of a composition product：By the freeze-drying of the freeze-dried powder of dendrobium leaf hydrolyzate, Hericium erinaceus hydrolyzate
Powder, embryo nelumbinis freeze-dried powder, hawthorn freeze-dried powder and sugar-acids mouthfeel instrumentality are mixed according to mass fraction, are stirred evenly, and are made each
Kind dosage form product, including pulvis, granule, pill, tincture or aqua.
Embodiment 2:
1, dendrobium leaf pre-processes and cellulase degradation process flow
Dendrobium leaf sorting → cutting → weighing → plus water → mashing → enzyme hydrolysis → filtering → detection
Process description：
(1) raw material is selected：Take fresh dendrobium leaf free from insect pests damaged portion.
(2) it is divided into fritter：Fresh dendrobium leaf is divided into fritter with pocket knife, is put into blender and facilitates crushing.
(3) it weighs：Fresh dendrobium leaf is put into specified containers, is weighed with electronic balance.
(4) add water：With fresh dendrobium leaf with 1g：The material-water ratio of 10ml, is deployed.
(5) it crushes：Fresh dendrobium leaf is put into blender, is quickly beaten, every beat stops primary, progress 3-4 for 10 seconds
Secondary whipping.
(6) enzyme hydrolysis：Five groups of hydrolysis temperature, enzymatic hydrolysis pH, enzyme concentration, enzymolysis time factors are carried out respectively to be tested.
(7) soluble solid is analyzed:Soluble solid analysis is carried out using abbe's refractometer
The experimental result of solid-liquid ratio is as shown in table 1.
The experiment of single factor of solid-liquid ratio during dendrobium leaf cellulose hydrolyzation is 50 DEG C, when enzymatic hydrolysis according to hydrolysis temperature
Between be 1h, enzymatic hydrolysis pH be 7.0, enzyme concentration be feed liquid gross mass 1%, take 1g：80ml,1g：100ml,1g：120ml,1g：
Tetra- groups of solid-liquid ratios of 140ml, carry out four groups of experiments respectively, finally compare soluble solid content, experiment knot with hand-held saccharometer
Shown in fruit table 1.
Influence of the enzymatic hydrolysis solid-liquid ratio to soluble solid content in dendrobium leaf liquid as seen from the results in Table 1, with solid-liquid ratio
Raising soluble solid change rate be present downward trend, therefore solid-liquid ratio be 1g:When 80ml, soluble solid
Highest.Therefore the best cellulase degradation solid-liquid ratio 1g of dendrobium leaf:80ml.
Influence of 1 solid-liquid ratio of table to dendrobium leaf cellulose hydrolyzation effect
Embodiment 3:
3, animal postprandial blood sugar measures and the blood sugar decreasing effect of dendrobium leaf
3.1 determination method of blood sugar：
(1) capsule of the oral consumption containing quantitative material, after adopted using blood collecting pen by male rabbit ear end every 15 minutes
Blood；
(2) blood sugar test paper is inserted into mating blood glucose meter, waits several seconds, blood glucose meter start completion；
(3) blood sample is touched into blood sugar test paper blood inlet end mouth, drop of blood is inhaled into automatically；
(4) confirmation blood sampling volume is enough；
(5) it waits 8 seconds, screen shows blood glucose test results.
Mensuration program of 3.2 foods to reducing postprandial blood glucose：
(1) oral consumption contains quantitative white granulated sugar capsule, after every 15 minutes by a male rabbit ear end blood sampling blood glucose of measurement,
It measures 5 times altogether, as control；
(2) capsule of the oral consumption containing quantitative material, after every 15 minutes by a male rabbit ear end blood sampling blood glucose of measurement,
It measures 5 times altogether.Compared with the control group.
Experimental result is as shown in Figure 1.The result shows that large ear rabbit (hero) is after ingest quantitative sucrose and dendrobium leaf, blood glucose exists
Amplitude of variation is little in preceding 30min.Blood glucose is begun to decline after 30min, even lower than fasting blood-glucose.The result shows that dendrobium nobile in figure
The leaf hydrolyzate freeze-dried powder hypoglycemic effect duration is long, and blood glucose fall is big, and edible rear animal postprandial blood sugar is even lower than
Fasting blood-glucose, so dendrobium leaf blood sugar decreasing effect highly significant, adjusting in postprandial blood sugar has preferable effect in control process.
Claims (4)
1. a kind of with the composition containing dendrobium leaf for reducing postprandial blood sugar effect, it is characterised in that by following parts by weight
Raw material is prepared：
2. according to claim 1 a kind of with the composition containing dendrobium leaf for reducing postprandial blood sugar effect, feature
It is：The dosage form of composition can be pulvis, granule, pill, tablet, tincture or aqua.
3. according to claim 1 a kind of with the composition containing dendrobium leaf for reducing postprandial blood sugar effect, feature
It is：The sugar-acids mouthfeel Auto-regulator is made of sweetener and acid, and the mass ratio of sweetener and acid is 50-
800：1, the sweetener is the combination of any one or two kinds in sorbierite or protein sugar；The acid is lemon
The combination of lemon acid, any one or two kinds in malic acid.
4. a kind of preparation method with the composition containing dendrobium leaf for reducing postprandial blood sugar effect described in claim 1,
It is characterized by comprising following steps：
1) a preparation dendrobium leaf hydrolyzate and the step of freeze-dried powder, by the sorting of fresh dendrobium leaf, cleaning and it is drained after, press
According to mass ratio 1:1 adds water mashing, successively hydrolyzes dendrobium leaf using cellulase and pectase and prepares its homogenate, cellulase
Enzymatic hydrolysis condition is：Solid-liquid ratio is 1g：60-100ml, enzyme concentration are the 1.0-1.5%, hydrolysis temperature 40-60 of feed liquid gross mass
DEG C, enzymolysis time 2-8h, enzymatic hydrolysis pH are 5.0-9.0；The enzymatic hydrolysis condition of pectase is：Solid-liquid ratio is 1g：60-100ml；It is enzyme
Amount be feed liquid gross mass 0.3-0.8%, hydrolysis temperature be 40-60 DEG C, enzymolysis time 1-3h；Enzymatic hydrolysis pH is 3.0-7.0；Water
5~15min is boiled after solution；Cooling is placed on -80 DEG C of refrigerator freezings for 24 hours, then carries out freeze-drying process, sets condenser temperature -
60 DEG C, vacuum degree 1MPa, drying time 48h；Solid after freeze-drying is crushed with pulverizer, is collected after 60 meshes excessively spare；
2) a preparation Hericium erinaceus hydrolyzate and the step of freeze-dried powder；It after dry Hericium erinaceus cleaning, crushes, crosses 100 mesh and be classified
Sieve, screenings add water and cellulase to be hydrolyzed, and enzyme hydrolysis treatment conditions are：Material-water ratio 1g：40-80ml, enzyme concentration
For the 0.6-1.5% for expecting water gross mass, enzymolysis time 3-9h, hydrolysis temperature is 30-50 DEG C, pH value of solution 6.0-8.0, hydrolysis
After boil 5~15min；Cooling is placed on -80 DEG C of refrigerator freezings for 24 hours, then carries out freeze-drying process, sets condenser temperature -60
DEG C, vacuum degree 1MPa, drying time 48h；Solid after freeze-drying is crushed with pulverizer, is collected after 60 meshes excessively spare；
3) preparation step of the freeze-dried powder of an embryo nelumbinis：Embryo nelumbinis is placed in -80 DEG C of refrigerator freezings for 24 hours by sorting, cleaning,
Freeze-drying process is carried out again；- 60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h of setting；Solid powder after freeze-drying
Broken machine crushes, and collects after 60 meshes excessively spare.
4) preparation step of the freeze-dried powder of a hawthorn：Hawthorn is sorted, cleaning, is enucleated, after cutting, and it is cold to be placed in -80 DEG C of refrigerators
Freeze for 24 hours, then carry out freeze-drying process, sets -60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h；Consolidating after freeze-drying
Body is crushed with pulverizer, is collected after 60 meshes excessively spare；
5) preparation step of a composition product：By the freeze-dried powder of dendrobium leaf hydrolyzate, the freeze-dried powder of Hericium erinaceus hydrolyzate, lotus
Sub- core freeze-dried powder, hawthorn freeze-dried powder and sugar-acids mouthfeel instrumentality are mixed according to mass fraction, stir evenly, various dosage forms are made
Have reduce postprandial blood sugar effect the composition containing dendrobium leaf, the dosage form includes pulvis, granule, pill, tincture
Agent or aqua.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810516340.8A CN108851041A (en) | 2018-05-25 | 2018-05-25 | A kind of composition and preparation method thereof containing dendrobium leaf with reduction postprandial blood sugar effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810516340.8A CN108851041A (en) | 2018-05-25 | 2018-05-25 | A kind of composition and preparation method thereof containing dendrobium leaf with reduction postprandial blood sugar effect |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108851041A true CN108851041A (en) | 2018-11-23 |
Family
ID=64333833
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810516340.8A Pending CN108851041A (en) | 2018-05-25 | 2018-05-25 | A kind of composition and preparation method thereof containing dendrobium leaf with reduction postprandial blood sugar effect |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108851041A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109730320A (en) * | 2019-03-21 | 2019-05-10 | 安徽农业大学 | A kind of preparation method of dendrobium nobile instant powder |
| CN110432491A (en) * | 2019-08-12 | 2019-11-12 | 上海应用技术大学 | A kind of dendrobium leaf effervescent tablet and preparation method thereof |
| CN114601158A (en) * | 2022-04-03 | 2022-06-10 | 安徽农业大学 | Preparation method of dendrobium officinale leaf meal replacement powder with health care function |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106039027A (en) * | 2016-06-23 | 2016-10-26 | 云南润紫源生物科技股份有限公司 | Dendrobium candidum leaf lozenge as well as preparation method and application thereof |
| CN106692361A (en) * | 2017-02-14 | 2017-05-24 | 云南国威生物科技有限公司 | Fagopyrum tataricum composition, preparation method thereof, preparations and application |
-
2018
- 2018-05-25 CN CN201810516340.8A patent/CN108851041A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106039027A (en) * | 2016-06-23 | 2016-10-26 | 云南润紫源生物科技股份有限公司 | Dendrobium candidum leaf lozenge as well as preparation method and application thereof |
| CN106692361A (en) * | 2017-02-14 | 2017-05-24 | 云南国威生物科技有限公司 | Fagopyrum tataricum composition, preparation method thereof, preparations and application |
Non-Patent Citations (5)
| Title |
|---|
| 唐政等: "混合酶提法提取铁皮石斛中石斛多糖的优化工艺研究", 《北方园艺》 * |
| 张廷模等: "《中国石斛品汇集要》", 30 April 2018, 中国医药科技出版社 * |
| 王强虎等: "《糖尿病患者怎样稳定血糖》", 31 August 2017, 金盾出版社 * |
| 田心: "《跑赢糖尿病》", 31 January 2015, 吉林科学技术出版社 * |
| 罗晓妙: "《凉山州野生食用菌综合开发利用》", 31 December 2013, 四川大学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109730320A (en) * | 2019-03-21 | 2019-05-10 | 安徽农业大学 | A kind of preparation method of dendrobium nobile instant powder |
| CN110432491A (en) * | 2019-08-12 | 2019-11-12 | 上海应用技术大学 | A kind of dendrobium leaf effervescent tablet and preparation method thereof |
| CN114601158A (en) * | 2022-04-03 | 2022-06-10 | 安徽农业大学 | Preparation method of dendrobium officinale leaf meal replacement powder with health care function |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104855830A (en) | Edible grain, bean and potato coarse cereal rice composition and preparing method thereof | |
| CN104621495A (en) | Dragon fruit peel active dietary fiber powder and preparation method thereof | |
| Jayasuriya et al. | Oral hypoglycemic activity of culinary-medicinal mushrooms Pleurotus ostreatus and P. cystidiosus (higher basidiomycetes) in normal and alloxan-induced diabetic Wistar rats | |
| CN108851041A (en) | A kind of composition and preparation method thereof containing dendrobium leaf with reduction postprandial blood sugar effect | |
| CN103621884A (en) | Composite nutritional rice capable of providing various nutrients and manufacturing process thereof | |
| CN108041201A (en) | A kind of Fu tea plants powder and preparation method thereof | |
| KR101105591B1 (en) | Functional rice for blood sugar down and making method thereof | |
| CN103431492B (en) | Production method and product of plasmod-radix puerariae electuary | |
| Srivastava et al. | Effects of microwave, ultrasound, and various treatments on the reduction of antinutritional factors in elephant foot yam: A review | |
| CN114009777A (en) | Glucoraphanin and moringa oleifera extract composition buccal tablet and application thereof | |
| CN102488130B (en) | Desaccharified selenium-containing chewing tablet and preparation method thereof | |
| Rodiah et al. | Physico-chemical and antioxidant properties of mesocarp and exocarp from Borassus flabellifer. | |
| JP2012039980A (en) | Burdock tea leaf powder and method of manufacturing the same | |
| CN104055119A (en) | Women healthcare lozenges prepared by tea flowers and maca powder | |
| CN101455377A (en) | Raw fiber food and preparation method thereof | |
| KR20150053524A (en) | Dried Noodle Using Helianthus tuberosus and Dangjo Pepper and Their of Method | |
| CN101147541B (en) | Sweet potato fresh bine tip vermicelli and its preparation method | |
| KR100733130B1 (en) | manufacturing method of garlic snack | |
| CN104855831A (en) | Rice mate and preparation method thereof | |
| CN104921014A (en) | Natural edible plant micro powder product capable of improving nutrient releasing efficiency and eating safety and preparation method of natural edible plant micro powder product | |
| CN107624943A (en) | A kind of jerusalem artichoke pressed candy and preparation method thereof | |
| CN103876126B (en) | The healthy vegetarian diet of edible mushroom corn and production method thereof | |
| KR102219735B1 (en) | Manufacturing method for jam using andong yam and jam manufactured by the same | |
| CN113068842A (en) | Preparation method of pear dietary fiber | |
| KR20040063693A (en) | Functional milk product |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181123 |