CN108851041A - A kind of composition and preparation method thereof containing dendrobium leaf with reduction postprandial blood sugar effect - Google Patents
A kind of composition and preparation method thereof containing dendrobium leaf with reduction postprandial blood sugar effect Download PDFInfo
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- CN108851041A CN108851041A CN201810516340.8A CN201810516340A CN108851041A CN 108851041 A CN108851041 A CN 108851041A CN 201810516340 A CN201810516340 A CN 201810516340A CN 108851041 A CN108851041 A CN 108851041A
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- freeze
- dried powder
- dendrobium leaf
- blood sugar
- drying
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/09—Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L25/00—Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof
- A23L25/30—Mashed or comminuted products, e.g. pulp, pastes, meal, powders; Products made therefrom, e.g. blocks, flakes, snacks; Liquid or semi-liquid products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/10—General methods of cooking foods, e.g. by roasting or frying
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention provides a kind of with the composition containing dendrobium leaf for reducing postprandial blood sugar effect, is made of the raw material of following parts by weight:1-3 parts of the freeze-dried powder of dendrobium leaf hydrolyzate;1-3 parts of the freeze-dried powder of Hericium erinaceus hydrolyzate;1-3 parts of embryo nelumbinis freeze-dried powder;1-3 parts of hawthorn freeze-dried powder;1-3 parts of sugar-acids mouthfeel instrumentality.The present invention also provides the preparation methods of above-mentioned composition, cellulase and pectin enzyme hydrolysis and freeze-drying method including dendrobium leaf;The cellulose hydrolyzation and freeze-drying method of Hericium erinaceus;Embryo nelumbinis and hawthorn freeze-dried powder;Above-mentioned each material is proportionally mixed, composition is prepared into.Postprandial blood sugar can be effectively reduced in product of the present invention, and each material is dual-purpose of drug and food material, can be taken for a long time, safe and in good taste.Meanwhile the present invention has expanded the utilization scope of dendrobium leaf, sufficiently develops and uses other positions other than stem, improves the utilization rate of dendrobium nobile plant.
Description
Technical field
The invention belongs to field of food, are related to a kind of health food, specifically a kind of that there is reduction postprandial blood sugar to make
Composition and preparation method thereof containing dendrobium leaf.
Background technique
With closing on for aging society, chronic epidemic disease becomes main epidemic disease.Postprandial hyperglycemia is the only of macrovascular diseases
Vertical risk factor.Postprandial hyperglycemia and retinopathy risk increase, Carotid Intima-media Thickness increases, myocardial blood volume and
Myocardial blood flow decline, risk of cancer are increased and the elderly's cognitive impairment is related, can also cause oxidative stress, inflammation and endothelium
Dysfunction.Epidemiological study the result shows that:Reduce the generation that postprandial blood sugar can be effectively reduced sudden cardiac death.
There are many modes of control blood glucose:Low suger diet is beneficial to control postprandial blood sugar;Some drugs are to the postprandial blood of control
It is sugared preferable;Diet intervention, physical exertion and body weight control are still the foundation stone of blood glucose control.Regulation postprandial blood sugar drug include
Glycosidase inhibitor, Drugs Promoting Insulin Secretion, insulin and its insulin analog, glucagon-like peptide etc..Drug is controlled
It is higher to treat price, and takes for a long time with certain toxic side effect (such as hypoglycemia, weight gain and gastrointestinal reaction), makees
Method to control postprandial blood sugar throughout one's life has certain drawbacks.
Contemporary society, cardiovascular and cerebrovascular epidemic disease have become the major chronic epidemic disease of person in middle and old age.Safety on domestic market
The effective health care product for adjusting postprandial blood sugar is less, therefore the health product exploitation of the reduction postprandial blood sugar based on natural food,
Have great importance.
Summary of the invention
For above-mentioned technical problem in the prior art, the present invention provides a kind of containing with reduction postprandial blood sugar effect
There are the composition and preparation method thereof of dendrobium leaf, this combination containing dendrobium leaf with reduction postprandial blood sugar effect
Object and preparation method thereof, which will solve food in the prior art, reduces the ineffective technical problem of postprandial blood sugar.
The present invention provides a kind of with the composition containing dendrobium leaf for reducing postprandial blood sugar effect, by following parts by weight
Several raw materials are prepared:
Further, the dosage form of composition can be pulvis, granule, pill, tablet, tincture or aqua.
Further, the sugar-acids mouthfeel Auto-regulator is made of sweetener and acid, sweetener and acid
Mass ratio be 50-800:1, the sweetener is the combination of any one or two kinds in sorbierite or protein sugar;Institute
The acid stated is the combination of citric acid, any one or two kinds in malic acid.
The present invention also provides a kind of above-mentioned systems with the composition containing dendrobium leaf for reducing postprandial blood sugar effect
Preparation Method includes the following steps:
1) a preparation dendrobium leaf hydrolyzate and the step of freeze-dried powder, by the sorting of fresh dendrobium leaf, cleaning and drained
Afterwards, according to mass ratio 1:1 adds water mashing, successively hydrolyzes dendrobium leaf using cellulase and pectase and prepares its homogenate, fiber
The enzymatic hydrolysis condition of plain enzyme is:Solid-liquid ratio is 1g:60-100ml, enzyme concentration are the 1.0-1.5% of feed liquid gross mass, and hydrolysis temperature is
40-60 DEG C, enzymolysis time 2-8h, enzymatic hydrolysis pH is 5.0-9.0;The enzymatic hydrolysis condition of pectase is:Solid-liquid ratio is 1g:60-
100ml;Enzyme concentration is the 0.3-0.8% of feed liquid gross mass, hydrolysis temperature is 40-60 DEG C, enzymolysis time 1-3h;Digesting pH is
3.0-7.0;5~15min is boiled after hydrolysis;Cooling is placed on -80 DEG C of refrigerator freezings for 24 hours, then carries out freeze-drying process, sets
- 60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h;Solid after freeze-drying is crushed with pulverizer, is collected after crossing 60 meshes
It is spare;
2) a preparation Hericium erinaceus hydrolyzate and the step of freeze-dried powder;After dry Hericium erinaceus cleaning, crushes, cross 100 mesh
Classifying screen, screenings add water and cellulase to be hydrolyzed, and enzyme hydrolysis treatment conditions are:Material-water ratio 1g:40-80ml adds
Enzyme amount is the 0.6-1.5% for expecting water gross mass, and enzymolysis time 3-9h, hydrolysis temperature is 30-50 DEG C, pH value of solution 6.0-8.0,
5~15min is boiled after hydrolysis;Cooling is placed on -80 DEG C of refrigerator freezings for 24 hours, then carries out freeze-drying process, sets cold-trap temperature
- 60 DEG C, vacuum degree 1MPa, drying time 48h of degree;Solid after freeze-drying is crushed with pulverizer, is collected after 60 meshes excessively spare;
3) preparation step of the freeze-dried powder of an embryo nelumbinis:Embryo nelumbinis is placed in -80 DEG C of refrigerator freezings by sorting, cleaning
For 24 hours, then freeze-drying process is carried out;- 60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h of setting;Solid after freeze-drying
It is crushed, is collected after 60 meshes excessively spare with pulverizer.
4) preparation step of the freeze-dried powder of a hawthorn:Hawthorn is sorted, cleaning, is enucleated, after cutting, is placed in -80 DEG C of ice
Case freezes for 24 hours, then carries out freeze-drying process, sets -60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h;After freeze-drying
Solid crushed with pulverizer, cross 60 meshes after collect it is spare;
5) preparation step of a composition product:By the freeze-drying of the freeze-dried powder of dendrobium leaf hydrolyzate, Hericium erinaceus hydrolyzate
Powder, embryo nelumbinis freeze-dried powder, hawthorn freeze-dried powder and sugar-acids mouthfeel instrumentality are mixed according to mass fraction, are stirred evenly, and are made each
Kind dosage form has the composition containing dendrobium leaf for reducing postprandial blood sugar effect, and the dosage form includes pulvis, granule, ball
Agent, tincture or aqua.
The purpose of the present invention is developing a kind of composition for adjusting postprandial blood sugar, the Component Source in the composition is in food
Object has effects that inhibit postprandial blood sugar rapid increase, does not have illeffects to human body, and sensory properties is easy to be accepted.
To achieve the goals above, for the present invention by pharmacological techniques, biotechnology and foods processing technique develop one
Kind adjusts the food-based composition of postprandial blood sugar.
The present invention also provides the preparation methods of above-mentioned composition, the cellulase and pectin enzyme hydrolysis including dendrobium leaf and
Freeze-drying method;The cellulase and pectin enzyme hydrolyzate and freeze-drying method of Hericium erinaceus;Embryo nelumbinis and hawthorn freeze-dried powder
Preparation method and final composition product preparation step, product of the present invention is proportionally to mix above-mentioned each material, system
For at composition.
Dendrobium candidum (Dendrobiumofficinale Kimura et Migo) mainly has enhancing immune, antifatigue, anti-
The pharmacological actions such as oxidation, hypoglycemic, blood pressure lowering.Dendrobium leaf is the leaf of dendrobium candidum plant.Dendrobium nobile leaf is often used as food
With drug processing waste and abandon.Research shows that:Dendrobium candidum stem from leaf respectively contain higher different effective components, always
Polysaccharide focuses primarily upon stem, and total alkaloid and general flavone focus primarily upon leaf.Stem and Ye Jun can be used as edible medicinal resource and filled
Divide and utilizes.In antioxidant activity experiment, remove DPPH free radical the results show that leaf portion polysaccharide is substantially better than stem's polysaccharide,
Show its anti-oxidant application value with higher.But the hypoglycemic effect of dendrobium nobile leaf has not been reported.
Hericium erinaceus (Hericium ernaceus) is a kind of fungi, and hericium erinaceum polysaccharide is the main active of Hericium erinaceus.
Modern medicine and pharmacological many researchs have shown that hericium erinaceum polysaccharide has and improve immunity, antitumor, anti-aging, reducing blood lipid etc.
Different physiological roles.Polysaccharide and oligosaccharides in Hericium erinaceus have hypoglycemic effect.Terpene and phenols in Hericium erinaceus is to golden yellow
Staphylococcus, Escherichia coli, bacillus subtilis all have certain fungistatic effect.
Lotus Plumule (Nelumbinis plumula) is drying spire and radicle in the mature seed of nymphaeaceae plant lotus,
With clearing away the heart fire and tranquillizing, relieving cough and reducing sputum, hemostasis is anti-inflammatory and other effects.Chemical component in Lotus Plumule is mainly alkaloids, additionally
There are the ingredients such as flavonoids, polysaccharide and volatile oil, there are the multiple pharmacological effects such as reducing blood lipid, anti-oxidant, anti-inflammatory.Embryo nelumbinis water mentions
Object has in various degree escherichia coli, streptococcus, staphylococcus aureus, salmonella typhimurium, Pasteurella etc.
Bacteriostasis.Therefore by the bacteriostatic activity of addition embryo nelumbinis, microbial growth in product can be prevented, preservative is played
Effect.Therefore, embryo nelumbinis can not only provide nutriment, can also play the role of antioxidant and preservative, extend and protect
The matter phase avoids the use of exogenous additive.
Hawthorn (Crataegus pinnatifida) is the fruit of rosaceae may.It is rich in hawthorn
Flavonoids, terpene and vitamin substances have the function of blood pressure lowering, anticoagulation, anti-oxidant and protection endothelial cell.
This patent cooperates dendrobium leaf, Hericium erinaceus, embryo nelumbinis and hawthorn, adds in conjunction with pharmacological experiment technology, biology
Work technology and food processing means carry out product formula optimization, product processing and functional verification to it, and developing dendrobium leaf is using newly
The utilization rate of dendrobium nobile is improved in field, has great importance to the scientific and reasonable diet of the special populations such as dysglycemia.
The raw materials such as dendrobium leaf, Hericium erinaceus, embryo nelumbinis and hawthorn, traditional processing mode are that water mentions or alcohol extracting, again to extract
It is further processed.Since the water solubility of polysaccharide and triterpenes, Flavonoid substances has very big difference, water is mentioned or alcohol extracting can
Lead to a large amount of losses of other non-solubility ingredients.Therefore, cellulose hydrolyzation is used in this patent, on the one hand improves polysaccharide
Bioavilability, another aspect cellulose hydrolysis after triterpene substance can be released, finally collect hydrolysis after it is complete
Portion's liquid.Due to not extracting a certain substance individually, the loss of other function ingredient in extraction process is avoided.To the maximum extent
Remain the content of polysaccharide and triterpenes, Flavonoid substances.
The dendrobium candidum (Dendrobiumofficinale Kimura et Migo) of dendrobium leaf involved in the present invention it is green
Color blade.Dendrobium leaf hydrolyzate involved in the present invention is dendrobium leaf by cellulase and pectin enzyme hydrolysis and filtered liquid
State product, containing the various polysaccharide materials and the nutriments such as mineral element, protein in dendrobium leaf in solution, and meanwhile it is solvable
Property carbohydrate (oligosaccharide such as monosaccharide, oligosaccharides, dextrin and soluble polysaccharide) content obviously increase than not hydrolyzing blade, be mostly derived from stone
The degradation of cellulose and pectin substance in dry measure used in former times leaf.Cellulase involved in the present invention is that catalytic degradation cellulose generates glucose
It is main with the general name of one group of enzyme of low polymerization degree fiber, including endoglucanase, 1,4-BETA-D-glucancellobio-hydrolase and cellobiase 3
Component (SIGMA reagent, C1184 derive from Aspergillus niger).Pectase involved in the present invention is mixture,
CAS:9032-75-1, Sigma Products, 3 main components of pectase are pectinesterase (EC 3.1.1.11), poly- gala
Uronic acid enzyme (EC3.2.1.15), pectin lyase (EC 4.2.2.10), can make hydrolyzed pectin sucrose and galacturonic acid.
It is crushed after dendrobium leaf cleaning, weighs and wiring solution-forming carries out enzyme hydrolysis processing.
The Hericium erinaceus (Hericium ernaceus) that the present invention uses is the fructification after a kind of fungi is dry.The present invention
Hericium erinaceus hydrolyzate to be Hericium erinaceus contain in Hericium erinaceus by cellulose hydrolyzation and filtered liquid product, solution
The nutritional ingredients such as polysaccharide, Flavonoid substances, minerals, but soluble saccharide (monosaccharide, oligosaccharides, oligosaccharide and soluble polysaccharide) contains
Amount does not hydrolyze Hericium erinaceus than original and obviously increases, the cellulose degraded being mostly derived from fruit.Cellulose involved in the present invention
Enzyme is the general name for one group of enzyme that catalytic degradation cellulose generates glucose and low polymerization degree fiber, including endoglucanase, Portugal
3 main components of glycan excision enzyme and cellobiase (SIGMA reagent, C1184 derive from Aspergillus niger).Claim
After taking quantitative Hericium erinaceus slurries, wiring solution-forming carries out cellulase hydrolysis.
The Lotus Plumule (Nelumbinis plumula) that the present invention uses is dry in the mature seed of nymphaeaceae plant lotus
Dry spire and radicle.Embryo nelumbinis is placed in -80 DEG C of refrigerator freezings for 24 hours, then carry out freeze-drying process by sorting, cleaning.Setting
- 60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h.;Solid after freeze-drying is crushed with pulverizer, crosses 60 meshes.
The hawthorn (Crataegus pinnatifida) that the present invention uses is the fruit of rosaceae may.Hawthorn
After sorted, cleaning, stoning, cutting, it is placed in -80 DEG C of refrigerator freezings for 24 hours, then carry out freeze-drying process.Set condenser temperature-
60 DEG C, vacuum degree 1MPa, drying time 48h.;Solid after freeze-drying is crushed with pulverizer, crosses 60 meshes.
Object of the present invention is to carry out compatibility by dendrobium leaf, Hericium erinaceus, embryo nelumbinis and hawthorn, using pharmacological outcomes as matching
The Testing index of Fang Youhua develops a kind of product for reducing postprandial blood sugar, reaches nutrition and functional complementation, and improve digestion
And absorptivity, and solid form facilitates storage and transportation or the addition in other food as primary products.
Present invention employs the combination of the freeze-dried powder of dendrobium leaf, the cellulase of Hericium erinaceus and pectin protease hydrolysate, nutrition
And have complementary functions, on the one hand enhance trophism and functionality, enhance hypoglycemic healthcare function, improve more in product
The digestibility and absorptivity of carbohydrate and cellulose substances;Free state polysaccharide in finished product, triterpene substance, Huang are improved simultaneously
Letones and other function content of material, enhance functional components, and the Flavonoid substances in embryo nelumbinis and hawthorn have
Antibacterial antioxidation can extend the shelf-life of final products in the case where not outer adding preservative agent and antioxidant.It reduces
The usage amount of antioxidant and preservative in product.
The composition sour and sweet palatability of method preparation of the invention, no bitter taste, color yellowish white is direct-edible, or
Beverage is diluted to liquid (water or ethyl alcohol) to eat, and be can also be used as ingredient and is added in other food.
The present invention is compared with prior art, and technological progress is significant.Present invention employs cellulases and pectase
Hydrolysis, on the one hand improves the bioavilability of insoluble polysaccharide in dendrobium leaf and Hericium erinaceus;On the other hand, cellulose or pectin
Triterpene substance, Flavonoid substances and the phenolic substances etc. in dendrobium leaf and Hericium erinaceus can be released after hydrolysis, not only be had
Effect of functional complementation also has and cooperates with anti-oxidant and anti-aging effects.The technology of the present invention is simple, noresidue, pollution-free, no
It is limited by room and time, be there is no adverse effect to processing space facility and people.
Detailed description of the invention
Fig. 1 shows influence of the dendrobium leaf hydrolyzate freeze-dried powder to animal postprandial blood sugar.
Specific embodiment
Embodiment 1:
The present invention provides a kind of with the composition containing dendrobium leaf for reducing postprandial blood sugar effect, by following parts by weight
Several raw materials are prepared:
Further, dosage form can be pulvis, granule, pill, tablet, tincture or aqua.
Further, the sugar-acids mouthfeel Auto-regulator is made of sweetener and acid, sweetener and acid
Mass ratio be 50-800:1, the sweetener is the combination of sorbierite, any one or two kinds in protein sugar;Described
Acid is the combination of any one or two kinds in citric acid or malic acid.
The present invention also provides a kind of above-mentioned systems with the composition containing dendrobium leaf for reducing postprandial blood sugar effect
Preparation Method includes the following steps:
1) a preparation dendrobium leaf hydrolyzate and the step of freeze-dried powder, by the sorting of fresh dendrobium leaf, cleaning and drained
Afterwards, according to mass ratio 1:1 adds water mashing, successively hydrolyzes dendrobium leaf using cellulase and pectase and prepares its homogenate, fiber
The enzymatic hydrolysis condition of plain enzyme is:Solid-liquid ratio is 1g:60-100ml, enzyme concentration are the 1.0-1.5% of feed liquid gross mass, and hydrolysis temperature is
40-60 DEG C, enzymolysis time 2-8h, enzymatic hydrolysis pH is 5.0-9.0;The enzymatic hydrolysis condition of pectase is:Solid-liquid ratio is 1g:60-
100ml;Enzyme concentration is the 0.3-0.8% of feed liquid gross mass, hydrolysis temperature is 40-60 DEG C, enzymolysis time 1-3h;Digesting pH is
3.0-7.0;5~15min is boiled after hydrolysis;Cooling is placed on -80 DEG C of refrigerator freezings for 24 hours, then carries out freeze-drying process, sets
- 60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h;Solid after freeze-drying is crushed with pulverizer, is collected after crossing 60 meshes
It is spare;
2) a preparation Hericium erinaceus hydrolyzate and the step of freeze-dried powder;After dry Hericium erinaceus cleaning, crushes, cross 100 mesh
Classifying screen, screenings add water and cellulase to be hydrolyzed, and enzyme hydrolysis treatment conditions are:Material-water ratio 1g:40-80ml adds
Enzyme amount is the 0.6-1.5% for expecting water gross mass, and enzymolysis time 3-9h, hydrolysis temperature is 30-50 DEG C, pH value of solution 6.0-8.0,
5~15min is boiled after hydrolysis;Cooling is placed on -80 DEG C of refrigerator freezings for 24 hours, then carries out freeze-drying process, sets cold-trap temperature
- 60 DEG C, vacuum degree 1MPa, drying time 48h of degree;Solid after freeze-drying is crushed with pulverizer, is collected after 60 meshes excessively spare;
3) preparation step of the freeze-dried powder of an embryo nelumbinis:Embryo nelumbinis is placed in -80 DEG C of refrigerator freezings by sorting, cleaning
For 24 hours, then freeze-drying process is carried out;- 60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h of setting;Solid after freeze-drying
It is crushed, is collected after 60 meshes excessively spare with pulverizer.
4) preparation step of the freeze-dried powder of a hawthorn:Hawthorn is sorted, cleaning, is enucleated, after cutting, is placed in -80 DEG C of ice
Case freezes for 24 hours, then carries out freeze-drying process, sets -60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h;After freeze-drying
Solid crushed with pulverizer, cross 60 meshes after collect it is spare;
5) preparation step of a composition product:By the freeze-drying of the freeze-dried powder of dendrobium leaf hydrolyzate, Hericium erinaceus hydrolyzate
Powder, embryo nelumbinis freeze-dried powder, hawthorn freeze-dried powder and sugar-acids mouthfeel instrumentality are mixed according to mass fraction, are stirred evenly, and are made each
Kind dosage form product, including pulvis, granule, pill, tincture or aqua.
Embodiment 2:
1, dendrobium leaf pre-processes and cellulase degradation process flow
Dendrobium leaf sorting → cutting → weighing → plus water → mashing → enzyme hydrolysis → filtering → detection
Process description:
(1) raw material is selected:Take fresh dendrobium leaf free from insect pests damaged portion.
(2) it is divided into fritter:Fresh dendrobium leaf is divided into fritter with pocket knife, is put into blender and facilitates crushing.
(3) it weighs:Fresh dendrobium leaf is put into specified containers, is weighed with electronic balance.
(4) add water:With fresh dendrobium leaf with 1g:The material-water ratio of 10ml, is deployed.
(5) it crushes:Fresh dendrobium leaf is put into blender, is quickly beaten, every beat stops primary, progress 3-4 for 10 seconds
Secondary whipping.
(6) enzyme hydrolysis:Five groups of hydrolysis temperature, enzymatic hydrolysis pH, enzyme concentration, enzymolysis time factors are carried out respectively to be tested.
(7) soluble solid is analyzed:Soluble solid analysis is carried out using abbe's refractometer
The experimental result of solid-liquid ratio is as shown in table 1.
The experiment of single factor of solid-liquid ratio during dendrobium leaf cellulose hydrolyzation is 50 DEG C, when enzymatic hydrolysis according to hydrolysis temperature
Between be 1h, enzymatic hydrolysis pH be 7.0, enzyme concentration be feed liquid gross mass 1%, take 1g:80ml,1g:100ml,1g:120ml,1g:
Tetra- groups of solid-liquid ratios of 140ml, carry out four groups of experiments respectively, finally compare soluble solid content, experiment knot with hand-held saccharometer
Shown in fruit table 1.
Influence of the enzymatic hydrolysis solid-liquid ratio to soluble solid content in dendrobium leaf liquid as seen from the results in Table 1, with solid-liquid ratio
Raising soluble solid change rate be present downward trend, therefore solid-liquid ratio be 1g:When 80ml, soluble solid
Highest.Therefore the best cellulase degradation solid-liquid ratio 1g of dendrobium leaf:80ml.
Influence of 1 solid-liquid ratio of table to dendrobium leaf cellulose hydrolyzation effect
Embodiment 3:
3, animal postprandial blood sugar measures and the blood sugar decreasing effect of dendrobium leaf
3.1 determination method of blood sugar:
(1) capsule of the oral consumption containing quantitative material, after adopted using blood collecting pen by male rabbit ear end every 15 minutes
Blood;
(2) blood sugar test paper is inserted into mating blood glucose meter, waits several seconds, blood glucose meter start completion;
(3) blood sample is touched into blood sugar test paper blood inlet end mouth, drop of blood is inhaled into automatically;
(4) confirmation blood sampling volume is enough;
(5) it waits 8 seconds, screen shows blood glucose test results.
Mensuration program of 3.2 foods to reducing postprandial blood glucose:
(1) oral consumption contains quantitative white granulated sugar capsule, after every 15 minutes by a male rabbit ear end blood sampling blood glucose of measurement,
It measures 5 times altogether, as control;
(2) capsule of the oral consumption containing quantitative material, after every 15 minutes by a male rabbit ear end blood sampling blood glucose of measurement,
It measures 5 times altogether.Compared with the control group.
Experimental result is as shown in Figure 1.The result shows that large ear rabbit (hero) is after ingest quantitative sucrose and dendrobium leaf, blood glucose exists
Amplitude of variation is little in preceding 30min.Blood glucose is begun to decline after 30min, even lower than fasting blood-glucose.The result shows that dendrobium nobile in figure
The leaf hydrolyzate freeze-dried powder hypoglycemic effect duration is long, and blood glucose fall is big, and edible rear animal postprandial blood sugar is even lower than
Fasting blood-glucose, so dendrobium leaf blood sugar decreasing effect highly significant, adjusting in postprandial blood sugar has preferable effect in control process.
Claims (4)
1. a kind of with the composition containing dendrobium leaf for reducing postprandial blood sugar effect, it is characterised in that by following parts by weight
Raw material is prepared:
2. according to claim 1 a kind of with the composition containing dendrobium leaf for reducing postprandial blood sugar effect, feature
It is:The dosage form of composition can be pulvis, granule, pill, tablet, tincture or aqua.
3. according to claim 1 a kind of with the composition containing dendrobium leaf for reducing postprandial blood sugar effect, feature
It is:The sugar-acids mouthfeel Auto-regulator is made of sweetener and acid, and the mass ratio of sweetener and acid is 50-
800:1, the sweetener is the combination of any one or two kinds in sorbierite or protein sugar;The acid is lemon
The combination of lemon acid, any one or two kinds in malic acid.
4. a kind of preparation method with the composition containing dendrobium leaf for reducing postprandial blood sugar effect described in claim 1,
It is characterized by comprising following steps:
1) a preparation dendrobium leaf hydrolyzate and the step of freeze-dried powder, by the sorting of fresh dendrobium leaf, cleaning and it is drained after, press
According to mass ratio 1:1 adds water mashing, successively hydrolyzes dendrobium leaf using cellulase and pectase and prepares its homogenate, cellulase
Enzymatic hydrolysis condition is:Solid-liquid ratio is 1g:60-100ml, enzyme concentration are the 1.0-1.5%, hydrolysis temperature 40-60 of feed liquid gross mass
DEG C, enzymolysis time 2-8h, enzymatic hydrolysis pH are 5.0-9.0;The enzymatic hydrolysis condition of pectase is:Solid-liquid ratio is 1g:60-100ml;It is enzyme
Amount be feed liquid gross mass 0.3-0.8%, hydrolysis temperature be 40-60 DEG C, enzymolysis time 1-3h;Enzymatic hydrolysis pH is 3.0-7.0;Water
5~15min is boiled after solution;Cooling is placed on -80 DEG C of refrigerator freezings for 24 hours, then carries out freeze-drying process, sets condenser temperature -
60 DEG C, vacuum degree 1MPa, drying time 48h;Solid after freeze-drying is crushed with pulverizer, is collected after 60 meshes excessively spare;
2) a preparation Hericium erinaceus hydrolyzate and the step of freeze-dried powder;It after dry Hericium erinaceus cleaning, crushes, crosses 100 mesh and be classified
Sieve, screenings add water and cellulase to be hydrolyzed, and enzyme hydrolysis treatment conditions are:Material-water ratio 1g:40-80ml, enzyme concentration
For the 0.6-1.5% for expecting water gross mass, enzymolysis time 3-9h, hydrolysis temperature is 30-50 DEG C, pH value of solution 6.0-8.0, hydrolysis
After boil 5~15min;Cooling is placed on -80 DEG C of refrigerator freezings for 24 hours, then carries out freeze-drying process, sets condenser temperature -60
DEG C, vacuum degree 1MPa, drying time 48h;Solid after freeze-drying is crushed with pulverizer, is collected after 60 meshes excessively spare;
3) preparation step of the freeze-dried powder of an embryo nelumbinis:Embryo nelumbinis is placed in -80 DEG C of refrigerator freezings for 24 hours by sorting, cleaning,
Freeze-drying process is carried out again;- 60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h of setting;Solid powder after freeze-drying
Broken machine crushes, and collects after 60 meshes excessively spare.
4) preparation step of the freeze-dried powder of a hawthorn:Hawthorn is sorted, cleaning, is enucleated, after cutting, and it is cold to be placed in -80 DEG C of refrigerators
Freeze for 24 hours, then carry out freeze-drying process, sets -60 DEG C of condenser temperature, vacuum degree 1MPa, drying time 48h;Consolidating after freeze-drying
Body is crushed with pulverizer, is collected after 60 meshes excessively spare;
5) preparation step of a composition product:By the freeze-dried powder of dendrobium leaf hydrolyzate, the freeze-dried powder of Hericium erinaceus hydrolyzate, lotus
Sub- core freeze-dried powder, hawthorn freeze-dried powder and sugar-acids mouthfeel instrumentality are mixed according to mass fraction, stir evenly, various dosage forms are made
Have reduce postprandial blood sugar effect the composition containing dendrobium leaf, the dosage form includes pulvis, granule, pill, tincture
Agent or aqua.
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CN109730320A (en) * | 2019-03-21 | 2019-05-10 | 安徽农业大学 | A kind of preparation method of dendrobium nobile instant powder |
CN110432491A (en) * | 2019-08-12 | 2019-11-12 | 上海应用技术大学 | A kind of dendrobium leaf effervescent tablet and preparation method thereof |
CN114601158A (en) * | 2022-04-03 | 2022-06-10 | 安徽农业大学 | Preparation method of dendrobium officinale leaf meal replacement powder with health care function |
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