CN101921345A - Method for extracting polysaccharide and flavone from bamboo leaves - Google Patents
Method for extracting polysaccharide and flavone from bamboo leaves Download PDFInfo
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- CN101921345A CN101921345A CN 201010252986 CN201010252986A CN101921345A CN 101921345 A CN101921345 A CN 101921345A CN 201010252986 CN201010252986 CN 201010252986 CN 201010252986 A CN201010252986 A CN 201010252986A CN 101921345 A CN101921345 A CN 101921345A
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- polysaccharide
- flavone
- bamboo leaves
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Abstract
The invention discloses a method for extracting polysaccharide and flavone from bamboo leaves, which is characterized by comprising the following steps of: adding the bamboo leaves into an extruding cavity of an extruder, controlling the temperature in the cavity to between 105 and 160 DEG C, and controlling the pressure to between 3 and 15 MPa to obtain the mixed liquid of the polysaccharide and the flavone. Compared with the conventional method, the method for extracting the polysaccharide and the flavone from the bamboo leaves has roughly equivalent yield, saves solvents and time cost, and has remarkable effect; the method for extracting the polysaccharide and the flavone from the bamboo leaves serving as a novel extraction method has the characteristics of rapidness, high efficiency, no use or low use of reagents as possible and the like, minimizes environmental pollution, is easy to continuously operate, meets the requirement of large-scale industrial production, breaks through the separation and the purification of natural products, and has important significance for radically changing the low development level, the extensive production mode, the out-dated technology for natural products in China.
Description
Technical field
The invention belongs to the extraction field of natural product, particularly the extraction of polysaccharide, flavones.
Background technology
The extracting method of present domestic natural product mainly contains methods such as solvent method, ultrasonic method, microwave method, enzyme process, macroporous resin adsorption isolation technique, supercritical fluid extraction, high speed adverse current chromatogram extraction and aqueous two phase extraction technique, and in the majority with traditional solvent extration.
But traditional extraction and separation method generally all has low or leaching process time of extraction efficiency long or solvent load is big or complex operation or be unfavorable for the extraction of thermally labile component and drawback that cost is high etc.And the product that extracts of conventional solvent, generally there is the dissolvent residual problem.
Summary of the invention
The objective of the invention is to propose a kind of method of extracting leaf of bamboo polysaccharide and flavones by extruding.
The present invention is based on heat, the pressure effect of extrusion technique, characteristic at high fibre contents of raw material such as mao bamboons, modified to containing coarse-fibred material extruding, extract the natural product of different physico-chemical properties in the material at short notice comprehensively, efficiently, all sidedly, in the hope of realizing the industrial applications of extrusion technique in natural product extraction.
Specifically, the present invention is achieved by the following technical solutions.
Adopt screw Extrusion Technology, the leaf of bamboo is added in the extrusion chamber of extrusion machine, the intravital temperature in chamber is controlled at 105 ℃-160 ℃, and pressure is 3-15MPa, obtains the mixing liquid of polysaccharide and flavones.
The present invention also can once wash the bamboo slag after the extruding, adds in the squeezed fluid again, and the feed liquid mass ratio of washing is 1: 3-5 is good with 1: 4 effect.
On the basis of the mixing liquid of polysaccharide and flavones, available prior art is separated polysaccharide with flavones.
As can be seen from the table, extrusion process extraction acquisition polysaccharide and flavones have all surpassed the economic development utility value lower limit more than 0.1%.Simultaneously, carry out after an auxiliary water carries pushing back bamboo slag, the polysaccharide total amount is compared with traditional method and is almost wanted to work as; The flavones total amount then accounts for 45%-65%, compares with traditional method, and yield is about the same.But extrusion process has been saved solvent and time cost.In a word, the extrusion process effect is obvious.
The present invention has fast, efficiently and as far as possible uses no or little characteristics such as reagent as a kind of novel extractive technique, and bottom line is reduced in environmental pollution, realizes operate continuously easily, is fit to the requirement of large-scale commercial production.On the Separation of Natural Products purifying, make a breakthrough, low to thorough change China natural product exploitation level, the mode of production is extensive, backward in technique etc. playing an important role, and is significant to China's plant utilization simultaneously.
Embodiment
The present invention will be described further by following examples.
Polysaccharide determination method of the present invention adopts the phenolsulfuric acid colorimetry, and described content of total flavone is measured and adopted aluminum nitrate complexing spectrophotometry.
Embodiment 1.
The leaf of bamboo is added in the chamber of screw extruder, the intravital temperature in chamber is controlled at 105 ℃, and pressure-controlling obtains the mixing liquid of polysaccharide and flavones at 3MPa, and wherein polysaccharide is 0.153%, and flavones is 0.107%.
Bamboo slag after the extruding is once washed, added in the squeezed fluid again, the feed liquid mass ratio of washing is 1: 3.Getting the polysaccharide total amount is 0.328%, and the flavones total amount is 0.282%.
Embodiment 2.
The leaf of bamboo is added in the chamber of screw extruder, the intravital temperature in chamber is controlled at 160 ℃, and pressure-controlling obtains the mixing liquid of polysaccharide and flavones at 12MPa, and wherein polysaccharide is 0.179%, and flavones is 0.103%.
Bamboo slag after the extruding is once washed, added in the squeezed fluid again, the feed liquid mass ratio of washing is 1: 5.Getting the polysaccharide total amount is 0.379%, and the flavones total amount is 0.302%.
Embodiment 3.
The leaf of bamboo is added in the chamber of screw extruder, the intravital temperature in chamber is controlled at 115 ℃, and pressure-controlling obtains the mixing liquid of polysaccharide and flavones at 15MPa, and wherein polysaccharide is 0.176%, and flavones is 0.115%.
Bamboo slag after the extruding is once washed, added in the squeezed fluid again, the feed liquid mass ratio of washing is 1: 4.Getting the polysaccharide total amount is 0.388%, and the flavones total amount is 0.334%.
Embodiment 4.
The leaf of bamboo is added in the chamber of screw extruder, the intravital temperature in chamber is controlled at 150 ℃, and pressure-controlling obtains the mixing liquid of polysaccharide and flavones at 5MPa, and polysaccharide is 0.171%, and flavones is 0.106%.
Bamboo slag after the extruding is once washed, added in the squeezed fluid again, the feed liquid mass ratio of washing is 1: 4.Getting the polysaccharide total amount is 0.359%, and the flavones total amount is 0.319%.
Claims (3)
1. a method of extracting leaf of bamboo polysaccharide and flavones is characterized in that the leaf of bamboo is added in the extrusion chamber of extrusion machine, and the intravital temperature in chamber is controlled at 105 ℃-160 ℃, and pressure-controlling gets the mixing liquid of polysaccharide and flavones at 3-15MPa.
2. method according to claim 1 is characterized in that the bamboo slag after the extruding is once washed, and adds in the squeezed fluid again, and the feed liquid mass ratio of washing is 1: 3-5.
3. method according to claim 2 is characterized in that the feed liquid mass ratio of washing is 1: 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010252986 CN101921345A (en) | 2010-08-13 | 2010-08-13 | Method for extracting polysaccharide and flavone from bamboo leaves |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010252986 CN101921345A (en) | 2010-08-13 | 2010-08-13 | Method for extracting polysaccharide and flavone from bamboo leaves |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101921345A true CN101921345A (en) | 2010-12-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010252986 Pending CN101921345A (en) | 2010-08-13 | 2010-08-13 | Method for extracting polysaccharide and flavone from bamboo leaves |
Country Status (1)
| Country | Link |
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| CN (1) | CN101921345A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1872884A (en) * | 2006-06-26 | 2006-12-06 | 甘肃奇正藏药有限公司 | Method for enriching beta glucan from highland barley |
| CN101519458A (en) * | 2009-03-26 | 2009-09-02 | 天津大学 | Method for extracting tea polysaccharides and tea polyphenol from coarse old green tea |
-
2010
- 2010-08-13 CN CN 201010252986 patent/CN101921345A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1872884A (en) * | 2006-06-26 | 2006-12-06 | 甘肃奇正藏药有限公司 | Method for enriching beta glucan from highland barley |
| CN101519458A (en) * | 2009-03-26 | 2009-09-02 | 天津大学 | Method for extracting tea polysaccharides and tea polyphenol from coarse old green tea |
Non-Patent Citations (1)
| Title |
|---|
| 《中国食品学报》 20100630 顾振宇等 挤压法提取竹叶多糖和黄酮的研究 1-3 第10卷, 第3期 2 * |
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Open date: 20101222 |