CA2369272A1 - Medicinal compositions and their method of preparation - Google Patents
Medicinal compositions and their method of preparation Download PDFInfo
- Publication number
- CA2369272A1 CA2369272A1 CA002369272A CA2369272A CA2369272A1 CA 2369272 A1 CA2369272 A1 CA 2369272A1 CA 002369272 A CA002369272 A CA 002369272A CA 2369272 A CA2369272 A CA 2369272A CA 2369272 A1 CA2369272 A1 CA 2369272A1
- Authority
- CA
- Canada
- Prior art keywords
- composition
- glycoalkaloid
- group
- bec
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 109
- 238000000034 method Methods 0.000 title claims description 47
- 238000002360 preparation method Methods 0.000 title claims description 25
- 229930008677 glyco alkaloid Natural products 0.000 claims abstract description 74
- 235000000346 sugar Nutrition 0.000 claims abstract description 54
- 150000008163 sugars Chemical class 0.000 claims abstract description 25
- 150000001875 compounds Chemical class 0.000 claims abstract description 17
- 230000003993 interaction Effects 0.000 claims abstract description 5
- 238000011282 treatment Methods 0.000 claims description 55
- 206010028980 Neoplasm Diseases 0.000 claims description 50
- 210000004027 cell Anatomy 0.000 claims description 32
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 27
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- -1 enol salts Chemical class 0.000 claims description 24
- 229930182470 glycoside Natural products 0.000 claims description 24
- KNLOWJPFLKGYGQ-UHFFFAOYSA-N Solasodine 3-O-??-L-rhamnopyranosyl (1‘Â∆2)-O-[??-D-glucopyranosyl (1‘Â∆4)]-??-D-glucopyranoside Natural products O1C2(NCC(C)CC2)C(C)C(C2(CCC3C4(C)CC5)C)C1CC2C3CC=C4CC5OC(C(C1O)OC2C(C(O)C(O)C(C)O2)O)OC(CO)C1OC1OC(CO)C(O)C(O)C1O KNLOWJPFLKGYGQ-UHFFFAOYSA-N 0.000 claims description 16
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- 239000007857 degradation product Substances 0.000 claims description 4
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
- A61K31/58—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids containing heterocyclic rings, e.g. danazol, stanozolol, pancuronium or digitogenin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
- A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
- A61K31/704—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
- A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/12—Keratolytics, e.g. wart or anti-corn preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
Abstract
A medicinal composition comprising at least one compound which can interact with a target cell, the at least one compound being a glycoalkaloid of general formula (I) wherein: the composition is essentially without free sugars of the type which inhibit the interaction between the at least one glycoalkaloid and a target cell.
Description
MEDICINAL COMPOSITIONS AND THEIR METHOD OF PREPARATION
FIELD OF THE INVENTION
The present invention relates to medicinal compositions and in particular therapeutic compositions comprising glycoalkaloids. Such compositions may be used in the treatment, control and diagnosis of cancers and tumors in mammals, contraception and termination of pregnancy. The present invention is particularly directed towards a composition comprising a mixture of solasodine glycosides.
The present invention is also directed towards a method of preparing a medicinal composition and a method of treatment, control or diagnosis of cancers and tumors in mammals.
BACKGROUND ART
Glycoalkaloids are steroidal alkaloids which have a sugar moiety bound to the alkaloid moiety. The sugar moiety can be a monosaccharide, disaccharide, oligosaccharide or polysaccharide. Certain glycoalkaloids derived from plants have been observed to have anti-cancer properties.
Of particular interest are glycoalkaloids extracted from the Solanum genus. Glycoalkaloids from the species Solanum Sodomaeum L. have been shown to be active against cancer in animals and skin tumors in humans. The glycoalkaloids extracted from the fruit of Solanum Sodomaeum L. include the triglycosides solasonine,[(22R, 25R) - spiro-5-en-3(3-yl-a-L-rhamnopyranosyl-(1->2ga1)-0-(3-D-glucopyranosyl-(1->3ga1)-(3-D-galactopyranose] (33%) , solamargine' (22R, 25R) -spiro-5-en-3(3-yl-a-L-rhamnopyranosyl-(1->2glu)-O-a-L-rhamnopyranosyl-(1->4glu)-(3-D-gluco-pyranose](33%), and their corresponding di- and monoglycosides (34%). All the glycosides contain the same aglycone, solasodine.
This mixture of glycosides which includes solasonine and solamargine is commonly referred to as BEC. The structures of solasonine and solamargine are shown below:
FIELD OF THE INVENTION
The present invention relates to medicinal compositions and in particular therapeutic compositions comprising glycoalkaloids. Such compositions may be used in the treatment, control and diagnosis of cancers and tumors in mammals, contraception and termination of pregnancy. The present invention is particularly directed towards a composition comprising a mixture of solasodine glycosides.
The present invention is also directed towards a method of preparing a medicinal composition and a method of treatment, control or diagnosis of cancers and tumors in mammals.
BACKGROUND ART
Glycoalkaloids are steroidal alkaloids which have a sugar moiety bound to the alkaloid moiety. The sugar moiety can be a monosaccharide, disaccharide, oligosaccharide or polysaccharide. Certain glycoalkaloids derived from plants have been observed to have anti-cancer properties.
Of particular interest are glycoalkaloids extracted from the Solanum genus. Glycoalkaloids from the species Solanum Sodomaeum L. have been shown to be active against cancer in animals and skin tumors in humans. The glycoalkaloids extracted from the fruit of Solanum Sodomaeum L. include the triglycosides solasonine,[(22R, 25R) - spiro-5-en-3(3-yl-a-L-rhamnopyranosyl-(1->2ga1)-0-(3-D-glucopyranosyl-(1->3ga1)-(3-D-galactopyranose] (33%) , solamargine' (22R, 25R) -spiro-5-en-3(3-yl-a-L-rhamnopyranosyl-(1->2glu)-O-a-L-rhamnopyranosyl-(1->4glu)-(3-D-gluco-pyranose](33%), and their corresponding di- and monoglycosides (34%). All the glycosides contain the same aglycone, solasodine.
This mixture of glycosides which includes solasonine and solamargine is commonly referred to as BEC. The structures of solasonine and solamargine are shown below:
H ~ C ~~~CH3 I
H0 Ol OH < I0 0 ~~0 HO
HO . 0 OH OH
~; ~~oH
CH3 So(asonine OH
H
HOC H
H3 C ..CH3 HOC ~
OH
~ ~0 0.%''~0 .
H,C 0 HO~ ~0 HO OH
OH OH OOH
cH~ Solama~gine HO
The anti-cancer properties of BEC has been studied in vivo with mice inoculated with murine sarcoma 180 and in cell culture studies. BEC was observed to selectively destroy tumor cells relative to normal cells.
The efficacy and specificity of BEC was also observed to be dependent upon the type of tumor. These observations were attributed to the presence of endogenous endocytic lectins or EEL' s present on the membranes of those cells observed to be susceptible to BEC. EEL's are endogenous receptors which have been reported to be expressed during human embryogenesis and carcinogenesis. Interaction of the EEL with a molecule or ligand for which it is a receptor results in internalization of the EEL and bound ligand.
It is believed that the tumor cells susceptible to BEC have EEL receptors specific for the glycoside portion of the glycoalkaloids in BEC. These EEL's selectively recognize and bind the sugar moiety of the glycoalkaloid. The glycoalkaloid is subsequently internalized and the result is destruction of the cell.
The mechanism of cell destruction is believed to be by cell lysis.
That there is an EEL specific for the glycoside moiety of the glycoalkaloid is supported by a number of observations. First, the aglycone, solasodine, when administered at levels at which BEC is effective is ineffective against tumor cells. The sugar portion of the glycoalkaloid on its own is also ineffective.
Second, competition studies have also shown that at least a three fold molar excess of the sugar rhamnose is required to inhibit the cytotoxicity of BEC.
Solamargine contains two molecules of rhamnose and solasonine, one molecule. It should be noted that rhamnose is a plant sugar and is rarely found in mammalian cells. Thus, it is unlikely that normal mammalian cells have a receptor for rhamnose.
The aforementioned competition studies were conducted on mice with murine sarcoma 180. Untreated mice died in 2-3 weeks. Four doses of 8mg/kg BEC given on consecutive days resulted in survival of virtually all animals. Five mg rhamnose/kg decreased the survival to 75%, lOmg rhamnose/kg decreased the survival to 50o and l5mg rhamnose/kg decreased the survival to 42%. Similar concentrations of rhamnose were observed to have no effect on 5180 activity in the absence of BEC.
Acute toxicity studies for BEC were also carried out in mice. These studies showed that for single intraperitoneal (ip) doses of BEC, the LDso was 30mg/kg. For administration of 14 daily single ip doses, the LDSO for mice was lOmg/kg. In contrast it was shown that the EDso (quantal effective level for 500 of the population after given a single dose) for a single dose of BEC was 9mg/kg. With 3 and 4 administrations at 9mg/kg BEC to mice with Sarcoma 180, greater than 95 0 of the mice were rendered cancer free for the remainder of their life span. The quantal effective levels (EDso) of BEC for single administrations were similar to the lethal levels (LDSO) for multiple administrations of lOmg/kg at 14 daily ip doses. BEC has also been observed to be effective for melanoma and ovarian tumor cells grown in cell culture. The therapeutic index (LDso/EDSO) for these cell culture trials was about 3.
It can be seen that a disadvantage of BEC is the toxicity of the preparation when administered at the very high levels required to successfully treat internal cancers. It would therefore be desirable to obtain a glycoalkaloid composition which is more effective than BEC for the treatment of cancers.
The above toxicity studies also provide further support for the EEL mediated activity of glycoalkaloids against cancer cells. Mice with advanced cancer activity could tolerate up to three times the LDloo of BEC. This could be explained by selective absorption of BEC by the cancer cells which were present in abundance. Thus the bioavailablity of BEC to normal cells could be reduced.
These toxicity studies also showed that ingestion of BEC
into normal cells can occur by routes other than selective recognition by EEL's at high concentrations.
For example, BEC at high concentrations may diffuse through the plasma membrane of the cell.
Treatment of premalignant and malignant skin lesions with BEC in humans has also been studied.
Topical application of BEC has been observed to be effective for the treatment of lesions consisting of keratosis, basal cell carcinoma and squamous cell carcinoma. Creams containing 10% and 0.005°s BEC when applied topically showed complete clinical and histological regression when applied twice daily over treatment periods of up to about three months. Although the final result of the 10% and 0.005% treatments were comparable in relation to regression of the disease, the duration of the treatment with the 0.005% BEC required for regression of the lesions was considerably longer 5 than for the 10% BEC. Typically the treatment period required for the low concentration of BEC was about 13 to 14 weeks.
The extended duration of the treatment for the low concentration BEC formulations has a number of disadvantages. First there is a difficulty with patient compliance. For optimum effectiveness, the BEC
formulation must be applied at regular intervals, typically twice a day, until clinical regression is observed. Many patients find it difficult to comply with such a regime for up to 14 weeks. During this period, patients may experience an unacceptable amount of pain due to high salicyclic acid concentrations. Further, during treatment, as the affected cells undergo lysis, the lesions ulcerate and should be covered by a dressing.
From a cosmetic and patient comfort perspective it would be desirable to be able to reduce the duration of treatment. Although such a reduction can be achieved by increasing the dose of BEC, this is undesirable in view of the toxicity of BEC. Still further, as large amounts of plant product are required to produce small amounts of BEC, the lOoBEC preparation is quite expensive to produce . It is therefore desirable to be able to obtain a low dose glycoalkaloid composition for the treatment of skin conditions, which results in clinical regression in a relatively short period of time and is also cost effective.
OBJECT OF THE INVENTION
It is therefore an object of the present invention to provide an improved glycoalkaloid composition for interaction with target cells and which may be used for the treatment of cancer and tumors in mammals and which may at least partially overcome the above disadvantages or provide the public with a useful choice.
SUMMARY OF THE INVENTION
Glycoalkaloids can undergo degradation in which the glycoside moiety or a saccharide unit thereof is cleaved from the alkaloid. Where the glycoside moiety of the glycoalkaloid includes two or more saccharide units, there are a number of possible degradation products including free sugars such as monosaccharides, disaccharides and trisaccharides; the aglycone and mono and diglycosides.
It has been surprisingly and unexpectedly discovered that the efficacy of a glycoalkaloid formulation against cancer, other abnormal cells or other target cells having EEL's can be inhibited by very low amounts of free sugars which may be produced as a result of degradation of the glycoalkaloid.
In the present specification and claims, the term "free sugars" refers to any sugar such as a mono, di, trisaccharide, oligosaccharide or polysaccharide or derivative thereof which is not bound to an alkaloid.
According to a first broad form of the invention, there is provided a medicinal composition comprising at least one compound which can interact with a target cell, the at least one compound being a glycoalkaloid of the general formula I:
R
A
3 o R3 i i r ~~ Rl Rl Rl ~R~ Rl wherein:
either one of the dotted lines represents a double bond, and the other a single bond, or both represent single bonds;
A: represents a radical selected from the following radicals of general formulae ( II ) to (V) R3 N R3 R3 X ~R3 R_'~O O
(II) (III)' H
or N ~O ORQ
(IV) (V) each of R1 is a radical separately selected from the group consisting of hydrogen, amino, oxo and OR4; each of Rz is a radical separately selected from the group consisting of hydrogen, amino and OR4~ each of R3 is a radical separately selected from the group consisting of hydrogen, alkyl and R4-alkylene; each of R4 is a radical separately selected from the group consisting of hydrogen, carbohydrate and a carbohydrate derivative;" X"
is a radical selected from the group comprising -CHZ-, -O-and -NHZ - ;
wherein the compound includes at least one R4 group in which R' is a carbohydrate or a derivative thereof;
together with a pharmaceutically acceptable carrier, adjuvant, excipient and/or diluent, wherein the composition is essentially without free sugars of the type which inhibit the interaction between the at least one glycoalkaloid and a target cell.
Preferred carbohydrate radicals R' are glyceric aldehyde; glycerose; erythrose; threose; ribose;
arabinose; xylose; lyxose; altrose; allose; gulose;
mannose; glucose; idose; galactose; talose; rhamnose;
dihydroxyactone; erythrulose; ribulose; xylulose;
psicose; fructose; sorbose; tagatose; and other hexoses ( C6H12O6 ) ; heptoses ( C,H140, ) ; octoses ( C8H1608 ) ; nanoses (C9H18O9) ; decoses (CloHzoOlo) : deoxysugars with branched chains (eg. apiose, hamamelose, streptose, cordycepose, mycarose and cladinose); compounds wherein the aldehyde, ketone or hydroxyl groups have been substituted (eg. N-acetyl, acetyl, methyl, replacement of CHZOH); sugar alcohols; sugar acids; benzimidazoles; the enol salts of the carbohydrates; saccharinic acids; sugar phosphates.
The more preferred compounds are solasonine, solamargine, solanine and tomatine.
Other preferred compounds of the general formula (1) are solanocapsine and 26-aminofurostane.
It will be appreciated that the various compounds referred to throughout this specification may be chiral and the present invention relates both to the individual stereoisomers and to any mixtures thereof including mixtures of enantiomers and/or diastereoisomers.
A preferred composition of the present invention is a solasodine glycoside composition which includes solasonine, solamargine and their di and monoglycosides in the same or similar proportion as the aforementioned BEC.
The composition of the present invention typically comprises naturally occurring glycoalkaloids extracted from a plant source. Generally, the plant extract is treated to remove essentially all of any free sugars which can inhibit the efficacy of the glycoalkaloids prior to formulation of the composition of the present invention. Although it may be possible that one or more free sugars do not inhibit the efficacy of the glycoalkaloids and do not need to be removed, typically all of the free sugars will be removed from the plant extract.
According to a further broad form of the invention there is provided a method of preparing a glycoalkaloid preparation comprising at least one glycoalkaloid according to formula I, as hereinbefore defined, the method including extracting the at least one glycoalkaloid from a suitable plant material to form a crude extract, and removing essentially all free sugars from the crude extract.
The crude extract may be obtained by any suitable method. When the plant material is Solanum Sodomaeum a preferred method is to extract coarsely ground plant material with acetic acid. The extract is filtered and the pH adjusted to about 9 to 10 to obtain a precipitate. The precipitate may be dissolved in acetic acid and re-precipitated at high pH. The precipitate is typically further extracted with ethanol to provide the solasodine glycoside mixture or BEC as a semicrystalline powder.
The free sugars may be removed from the plant extract by any suitable method. A preferred method is to wash the crude extract in water or other suitable solvent. Generally, the free sugars are removed to below detectable limits or are at least removed to a level below which an inhibitory effect can be detected.
Generally, the composition of the present invention is essentially without all free sugars. However, it will be appreciated that free sugars which do not inhibit the cytotoxicity of the glycoalkaloids may be present.
The composition of the present invention may also be formulated from a synthetic glycoalkaloid or a mixture of glycoalkaloids. In this case, the synthetic glycoalkaloids would typically be treated prior to formulation of the composition to remove any sugars present as a result of glycoalkaloid degradation.
The glycoalkaloids in the composition of the present invention may also be obtained from chemical modification of naturally occurring glycoalkaloids. In this case, the naturally occurring sugar moiety of the 5 glycoalkaloid can be modified by removing or adding a saccharide unit or units. Suitable methods of carbohydrate modification are known and include chemical or enzymatic hydrolysis. Alternatively, the sugar moiety may be completely removed and replaced with a different 10 sugar moiety. An advantage of such modification of the sugar group of a glycoalkaloid is to be able to modify the efficacy or selectivity of that glycoalkaloid towards a desired target cell.
It is believed that the mode of action of glycoalkaloids against target cells is by EEL mediated endocytosis in which an EEL recognizes the sugar moiety of the glycoalkaloid and subsequent internalization of the EEL and glycoalkaloid. Thus, by identifying those sugars which can be recognized by receptors on a desired target cell, a modified glycoalkaloid may be derived which is specific to that receptor. In this way a glycoalkaloid can be designed to target a desired cell type.
The products of glycoalkaloid degradation may also include the aglycone. Preferably, any aglycone is also removed prior to formulation of the therapeutic compositions of the present invention. Removal of the aglycone may be conducted by any suitable means and is typically removed by solvent extraction. Suitable solvents include the chlorinated hydrocarbon solvents and chloroform is particularly preferred.
Under normal storage conditions, some degradation of glycoalkaloids in a pure or semi-pure crystalline or semicrystalline form can occur. Thus, it is preferred, that where storage has occurred, the aforementioned sugar removal and if desired aglycone removal of stored glycoalkaloid be conducted immediately prior to formulation of the therapeutic compositions of the invention. Typically the composition is stabilized against glycoalkaloid degradation. Typically, the composition is acidic and preferably includes acetic or lactic acid. The acidic conditions minimize degradation to produce free sugars.
Alternatively, sugar free glycoalkaloid preparations including the crystalline form may be prepared and then stored under stable conditions prior to formulation of the therapeutic composition of the present invention. The sugar free preparation may be stored in an acidic solution and/or at low temperature.
According to a further broad. form of the present invention, there is provided a method of preparing a therapeutic composition which comprises a therapeutically effective amount of at least one glycoalkaloid according to formula I, as hereinbefore defined, the method including obtaining at least one glycoalkaloid, removing any free sugars from the glycoalkaloid and mixing the glycoalkaloid with a pharmaceutically acceptable stabilizer.
The amount of the glycoalkaloid present in the therapeutic composition of the present invention may depend on the dose rate, patient, the type of condition being treated and in the case of a tumor the type, size and position of the tumor to be treated. In the preferred composition which includes solasodine glycosides, a typical composition for the treatment of skin tumors would typically include between about 5 to about 0.001%, preferably about 0.005% solasodine glycosides.
The therapeutic composition of the present invention may be used in the treatment and control of conditions which may be treated or controlled by selective cellular destruction or modification. Such uses include the treatment or control of cancer, contraception, termination of pregnancy, removal of pathogenic organisms and removal of abnormal cellular growth.
H0 Ol OH < I0 0 ~~0 HO
HO . 0 OH OH
~; ~~oH
CH3 So(asonine OH
H
HOC H
H3 C ..CH3 HOC ~
OH
~ ~0 0.%''~0 .
H,C 0 HO~ ~0 HO OH
OH OH OOH
cH~ Solama~gine HO
The anti-cancer properties of BEC has been studied in vivo with mice inoculated with murine sarcoma 180 and in cell culture studies. BEC was observed to selectively destroy tumor cells relative to normal cells.
The efficacy and specificity of BEC was also observed to be dependent upon the type of tumor. These observations were attributed to the presence of endogenous endocytic lectins or EEL' s present on the membranes of those cells observed to be susceptible to BEC. EEL's are endogenous receptors which have been reported to be expressed during human embryogenesis and carcinogenesis. Interaction of the EEL with a molecule or ligand for which it is a receptor results in internalization of the EEL and bound ligand.
It is believed that the tumor cells susceptible to BEC have EEL receptors specific for the glycoside portion of the glycoalkaloids in BEC. These EEL's selectively recognize and bind the sugar moiety of the glycoalkaloid. The glycoalkaloid is subsequently internalized and the result is destruction of the cell.
The mechanism of cell destruction is believed to be by cell lysis.
That there is an EEL specific for the glycoside moiety of the glycoalkaloid is supported by a number of observations. First, the aglycone, solasodine, when administered at levels at which BEC is effective is ineffective against tumor cells. The sugar portion of the glycoalkaloid on its own is also ineffective.
Second, competition studies have also shown that at least a three fold molar excess of the sugar rhamnose is required to inhibit the cytotoxicity of BEC.
Solamargine contains two molecules of rhamnose and solasonine, one molecule. It should be noted that rhamnose is a plant sugar and is rarely found in mammalian cells. Thus, it is unlikely that normal mammalian cells have a receptor for rhamnose.
The aforementioned competition studies were conducted on mice with murine sarcoma 180. Untreated mice died in 2-3 weeks. Four doses of 8mg/kg BEC given on consecutive days resulted in survival of virtually all animals. Five mg rhamnose/kg decreased the survival to 75%, lOmg rhamnose/kg decreased the survival to 50o and l5mg rhamnose/kg decreased the survival to 42%. Similar concentrations of rhamnose were observed to have no effect on 5180 activity in the absence of BEC.
Acute toxicity studies for BEC were also carried out in mice. These studies showed that for single intraperitoneal (ip) doses of BEC, the LDso was 30mg/kg. For administration of 14 daily single ip doses, the LDSO for mice was lOmg/kg. In contrast it was shown that the EDso (quantal effective level for 500 of the population after given a single dose) for a single dose of BEC was 9mg/kg. With 3 and 4 administrations at 9mg/kg BEC to mice with Sarcoma 180, greater than 95 0 of the mice were rendered cancer free for the remainder of their life span. The quantal effective levels (EDso) of BEC for single administrations were similar to the lethal levels (LDSO) for multiple administrations of lOmg/kg at 14 daily ip doses. BEC has also been observed to be effective for melanoma and ovarian tumor cells grown in cell culture. The therapeutic index (LDso/EDSO) for these cell culture trials was about 3.
It can be seen that a disadvantage of BEC is the toxicity of the preparation when administered at the very high levels required to successfully treat internal cancers. It would therefore be desirable to obtain a glycoalkaloid composition which is more effective than BEC for the treatment of cancers.
The above toxicity studies also provide further support for the EEL mediated activity of glycoalkaloids against cancer cells. Mice with advanced cancer activity could tolerate up to three times the LDloo of BEC. This could be explained by selective absorption of BEC by the cancer cells which were present in abundance. Thus the bioavailablity of BEC to normal cells could be reduced.
These toxicity studies also showed that ingestion of BEC
into normal cells can occur by routes other than selective recognition by EEL's at high concentrations.
For example, BEC at high concentrations may diffuse through the plasma membrane of the cell.
Treatment of premalignant and malignant skin lesions with BEC in humans has also been studied.
Topical application of BEC has been observed to be effective for the treatment of lesions consisting of keratosis, basal cell carcinoma and squamous cell carcinoma. Creams containing 10% and 0.005°s BEC when applied topically showed complete clinical and histological regression when applied twice daily over treatment periods of up to about three months. Although the final result of the 10% and 0.005% treatments were comparable in relation to regression of the disease, the duration of the treatment with the 0.005% BEC required for regression of the lesions was considerably longer 5 than for the 10% BEC. Typically the treatment period required for the low concentration of BEC was about 13 to 14 weeks.
The extended duration of the treatment for the low concentration BEC formulations has a number of disadvantages. First there is a difficulty with patient compliance. For optimum effectiveness, the BEC
formulation must be applied at regular intervals, typically twice a day, until clinical regression is observed. Many patients find it difficult to comply with such a regime for up to 14 weeks. During this period, patients may experience an unacceptable amount of pain due to high salicyclic acid concentrations. Further, during treatment, as the affected cells undergo lysis, the lesions ulcerate and should be covered by a dressing.
From a cosmetic and patient comfort perspective it would be desirable to be able to reduce the duration of treatment. Although such a reduction can be achieved by increasing the dose of BEC, this is undesirable in view of the toxicity of BEC. Still further, as large amounts of plant product are required to produce small amounts of BEC, the lOoBEC preparation is quite expensive to produce . It is therefore desirable to be able to obtain a low dose glycoalkaloid composition for the treatment of skin conditions, which results in clinical regression in a relatively short period of time and is also cost effective.
OBJECT OF THE INVENTION
It is therefore an object of the present invention to provide an improved glycoalkaloid composition for interaction with target cells and which may be used for the treatment of cancer and tumors in mammals and which may at least partially overcome the above disadvantages or provide the public with a useful choice.
SUMMARY OF THE INVENTION
Glycoalkaloids can undergo degradation in which the glycoside moiety or a saccharide unit thereof is cleaved from the alkaloid. Where the glycoside moiety of the glycoalkaloid includes two or more saccharide units, there are a number of possible degradation products including free sugars such as monosaccharides, disaccharides and trisaccharides; the aglycone and mono and diglycosides.
It has been surprisingly and unexpectedly discovered that the efficacy of a glycoalkaloid formulation against cancer, other abnormal cells or other target cells having EEL's can be inhibited by very low amounts of free sugars which may be produced as a result of degradation of the glycoalkaloid.
In the present specification and claims, the term "free sugars" refers to any sugar such as a mono, di, trisaccharide, oligosaccharide or polysaccharide or derivative thereof which is not bound to an alkaloid.
According to a first broad form of the invention, there is provided a medicinal composition comprising at least one compound which can interact with a target cell, the at least one compound being a glycoalkaloid of the general formula I:
R
A
3 o R3 i i r ~~ Rl Rl Rl ~R~ Rl wherein:
either one of the dotted lines represents a double bond, and the other a single bond, or both represent single bonds;
A: represents a radical selected from the following radicals of general formulae ( II ) to (V) R3 N R3 R3 X ~R3 R_'~O O
(II) (III)' H
or N ~O ORQ
(IV) (V) each of R1 is a radical separately selected from the group consisting of hydrogen, amino, oxo and OR4; each of Rz is a radical separately selected from the group consisting of hydrogen, amino and OR4~ each of R3 is a radical separately selected from the group consisting of hydrogen, alkyl and R4-alkylene; each of R4 is a radical separately selected from the group consisting of hydrogen, carbohydrate and a carbohydrate derivative;" X"
is a radical selected from the group comprising -CHZ-, -O-and -NHZ - ;
wherein the compound includes at least one R4 group in which R' is a carbohydrate or a derivative thereof;
together with a pharmaceutically acceptable carrier, adjuvant, excipient and/or diluent, wherein the composition is essentially without free sugars of the type which inhibit the interaction between the at least one glycoalkaloid and a target cell.
Preferred carbohydrate radicals R' are glyceric aldehyde; glycerose; erythrose; threose; ribose;
arabinose; xylose; lyxose; altrose; allose; gulose;
mannose; glucose; idose; galactose; talose; rhamnose;
dihydroxyactone; erythrulose; ribulose; xylulose;
psicose; fructose; sorbose; tagatose; and other hexoses ( C6H12O6 ) ; heptoses ( C,H140, ) ; octoses ( C8H1608 ) ; nanoses (C9H18O9) ; decoses (CloHzoOlo) : deoxysugars with branched chains (eg. apiose, hamamelose, streptose, cordycepose, mycarose and cladinose); compounds wherein the aldehyde, ketone or hydroxyl groups have been substituted (eg. N-acetyl, acetyl, methyl, replacement of CHZOH); sugar alcohols; sugar acids; benzimidazoles; the enol salts of the carbohydrates; saccharinic acids; sugar phosphates.
The more preferred compounds are solasonine, solamargine, solanine and tomatine.
Other preferred compounds of the general formula (1) are solanocapsine and 26-aminofurostane.
It will be appreciated that the various compounds referred to throughout this specification may be chiral and the present invention relates both to the individual stereoisomers and to any mixtures thereof including mixtures of enantiomers and/or diastereoisomers.
A preferred composition of the present invention is a solasodine glycoside composition which includes solasonine, solamargine and their di and monoglycosides in the same or similar proportion as the aforementioned BEC.
The composition of the present invention typically comprises naturally occurring glycoalkaloids extracted from a plant source. Generally, the plant extract is treated to remove essentially all of any free sugars which can inhibit the efficacy of the glycoalkaloids prior to formulation of the composition of the present invention. Although it may be possible that one or more free sugars do not inhibit the efficacy of the glycoalkaloids and do not need to be removed, typically all of the free sugars will be removed from the plant extract.
According to a further broad form of the invention there is provided a method of preparing a glycoalkaloid preparation comprising at least one glycoalkaloid according to formula I, as hereinbefore defined, the method including extracting the at least one glycoalkaloid from a suitable plant material to form a crude extract, and removing essentially all free sugars from the crude extract.
The crude extract may be obtained by any suitable method. When the plant material is Solanum Sodomaeum a preferred method is to extract coarsely ground plant material with acetic acid. The extract is filtered and the pH adjusted to about 9 to 10 to obtain a precipitate. The precipitate may be dissolved in acetic acid and re-precipitated at high pH. The precipitate is typically further extracted with ethanol to provide the solasodine glycoside mixture or BEC as a semicrystalline powder.
The free sugars may be removed from the plant extract by any suitable method. A preferred method is to wash the crude extract in water or other suitable solvent. Generally, the free sugars are removed to below detectable limits or are at least removed to a level below which an inhibitory effect can be detected.
Generally, the composition of the present invention is essentially without all free sugars. However, it will be appreciated that free sugars which do not inhibit the cytotoxicity of the glycoalkaloids may be present.
The composition of the present invention may also be formulated from a synthetic glycoalkaloid or a mixture of glycoalkaloids. In this case, the synthetic glycoalkaloids would typically be treated prior to formulation of the composition to remove any sugars present as a result of glycoalkaloid degradation.
The glycoalkaloids in the composition of the present invention may also be obtained from chemical modification of naturally occurring glycoalkaloids. In this case, the naturally occurring sugar moiety of the 5 glycoalkaloid can be modified by removing or adding a saccharide unit or units. Suitable methods of carbohydrate modification are known and include chemical or enzymatic hydrolysis. Alternatively, the sugar moiety may be completely removed and replaced with a different 10 sugar moiety. An advantage of such modification of the sugar group of a glycoalkaloid is to be able to modify the efficacy or selectivity of that glycoalkaloid towards a desired target cell.
It is believed that the mode of action of glycoalkaloids against target cells is by EEL mediated endocytosis in which an EEL recognizes the sugar moiety of the glycoalkaloid and subsequent internalization of the EEL and glycoalkaloid. Thus, by identifying those sugars which can be recognized by receptors on a desired target cell, a modified glycoalkaloid may be derived which is specific to that receptor. In this way a glycoalkaloid can be designed to target a desired cell type.
The products of glycoalkaloid degradation may also include the aglycone. Preferably, any aglycone is also removed prior to formulation of the therapeutic compositions of the present invention. Removal of the aglycone may be conducted by any suitable means and is typically removed by solvent extraction. Suitable solvents include the chlorinated hydrocarbon solvents and chloroform is particularly preferred.
Under normal storage conditions, some degradation of glycoalkaloids in a pure or semi-pure crystalline or semicrystalline form can occur. Thus, it is preferred, that where storage has occurred, the aforementioned sugar removal and if desired aglycone removal of stored glycoalkaloid be conducted immediately prior to formulation of the therapeutic compositions of the invention. Typically the composition is stabilized against glycoalkaloid degradation. Typically, the composition is acidic and preferably includes acetic or lactic acid. The acidic conditions minimize degradation to produce free sugars.
Alternatively, sugar free glycoalkaloid preparations including the crystalline form may be prepared and then stored under stable conditions prior to formulation of the therapeutic composition of the present invention. The sugar free preparation may be stored in an acidic solution and/or at low temperature.
According to a further broad. form of the present invention, there is provided a method of preparing a therapeutic composition which comprises a therapeutically effective amount of at least one glycoalkaloid according to formula I, as hereinbefore defined, the method including obtaining at least one glycoalkaloid, removing any free sugars from the glycoalkaloid and mixing the glycoalkaloid with a pharmaceutically acceptable stabilizer.
The amount of the glycoalkaloid present in the therapeutic composition of the present invention may depend on the dose rate, patient, the type of condition being treated and in the case of a tumor the type, size and position of the tumor to be treated. In the preferred composition which includes solasodine glycosides, a typical composition for the treatment of skin tumors would typically include between about 5 to about 0.001%, preferably about 0.005% solasodine glycosides.
The therapeutic composition of the present invention may be used in the treatment and control of conditions which may be treated or controlled by selective cellular destruction or modification. Such uses include the treatment or control of cancer, contraception, termination of pregnancy, removal of pathogenic organisms and removal of abnormal cellular growth.
According to a further broad form of the present invention there is provided a method for the treatment or control of cancer, contraception, termination of pregnancy, removal of pathogenic organisms and removal of abnormal cellular growth in a mammal requiring such treatment, the method comprising administering to the mammal an effective amount of a medicinal composition or preparation of the present invention.
The medicinal composition of the present invention may be formulated in any suitable manner including injectable compositions, tablets, suppositories, capsules and topical formulations.. In a preferred formulation for the treatment of skin tumors or lesions, the formulation is a cream for topical administration or an injectable formulation. In the case of an internal cancer or sarcoid, the composition may be an injectable formulation for intraperitoneal or intralesional injection.
Typically, the injectable composition is administered in an amount of between about 50 to about 200 mg of sugar free glycoalkaloid composition per kg of tumour. Animal and human studies (as illustrated in the following examples) show that successful treatment of some tumors and cancers may be accomplished with as few as two to four injections. The injection may be given at one, two or three daily intervals, preferably the treatment is given twice, at day 1 and day 3. Treatment by injection may also be given in association with topical administration if desired or considered necessary.
It has also been surprisingly discovered that the therapeutic composition of the present invention may also be used to diagnose skin conditions before such conditions can be detected by visual inspection.
Such diagnosis may be carried out by broadly applying a composition of the present invention to an area of skin to be tested. The composition is left on the skin for a pre-determined period of time. During this time, any abnormal cells are selectively destroyed.
This produces a detectable inflammation of the affected areas which may then be identified and treated.
According to a further broad form of the present invention there is provided a method of diagnosing a skin condition in a mammal, the skin condition being caused by the presence of abnormal cells, wherein the method includes applying an effective amount of a composition or preparation of the present invention to an area of skin to be diagnosed, leaving the composition on the skin for a pre-determined period of time, removing the composition and detecting any change to any areas of skin.
The diagnostic method of the present invention is particularly suitable for diagnosing skin conditions of humans. Typical conditions which may be diagnosed include Keratoses, basal cell carcinomas, squamous cell carcinomas, melanomas or other skin cancers.
A particularly preferred diagnostic composition is a solasodine glycoside mixture having about the same glycoside composition as BEC but without free sugars or the aglycone, solasodine. In trials conducted by the present inventor it has been observed that the normal healthy skin tissue is unaffected by the composition.
This demonstrates the selectivity of glycoalkaloids for abnormal cells.
This method of diagnosis allows skin conditions to be detected and treated at an early stage, typically before the condition produces visible skin lesions.
It should be appreciated that such a method of diagnosis would not be possible with conventional skin treatment compositions which adversely affect all cells.
A further advantage of such specificity is that during application, should the composition be inadvertently applied to a patients' healthy skin, the healthy skin will not be damaged. This does not occur with conventional skin treatment where care must be exercised to avoid contact with healthy skin.
Further, in view of the suprisingly improved efficacy of the present invention in treatment of skin conditions, the diagnosis can be conducted using very low concentrations of solasodine glycosides.
BRIEF DESCRIPTION OF THE DRAWINGS
Figures 1 and 3 and Figures 2 and 4 illustrate HPLC spectra for unwashed and washed BEC respectively.
Figures 5 to 8 illustrate a sarcoid tumor in a horse, before (Figure 5), during (Figures 6 and 7) and after (Figure 8) treatment with a preferred composition of the present invention.
Figures 9 to 12 illustrate the stages in the treatment of a horse with a penile sarcoma before (Figure 9) during (Figures 10 and 11) and after (Figure 12) treatment with a preferred composition of the present invention.
Figures 13 to 15 illustrate stages in the treatment of a human with squamous cell carcinoma before (Figure 13) and during (Figures 14 and 15) treatment.
BEST MODE
The present invention will now be described with reference to the following non-limiting examples.
Example 1 A sugar free solasodine glycoside preparation was prepared according to the following:
50kg Solanum Sodomaeum berries are put through commercial meat mincer (fitted with 1.HP electric motor 1425 rpm) with a sieve size of 3mm.
The slurry is diluted with 3o acetic acid (pH
2.5) (food grade) to a volume of 200L. This semi-solid solution is treated with a Silverson homogenizer for 15 minutes. Mixing is continued for another 4 hours using a SS rod with arms mixer at room temperature at 30 rpm (Flamingo CMG 0.75kw variable speed control meter).
The solution is allowed to stand overnight without mixing. The solution is subsequently filtered through a muslin cloth. The filtrate is then subjected to a flow through centrifuge (3.5HP) at 1455 rpm. The clear filtrate is heated to 50°C in a stainless steel double jacketed bowl. Concentrated ammonia (L R Grade) 5 is added until pH ~ 10. A precipitate is observed. The precipitate is allowed to settle and cool (approx. 24 hrs). The supernatant is carefully decanted. The precipitate is dissolved in 25L 3% aqueous acetic acid.
The solution is centrifuged through flow through 10 centrifuge as above. The supernatant is collected in an SS double jacketed bowl and heated to 50°C with continuous stirring (30 rpm, 30min).
The glycoalkaloids are re-precipitated by the addition of concentrated ammonia solution until pH ~ 10.
The medicinal composition of the present invention may be formulated in any suitable manner including injectable compositions, tablets, suppositories, capsules and topical formulations.. In a preferred formulation for the treatment of skin tumors or lesions, the formulation is a cream for topical administration or an injectable formulation. In the case of an internal cancer or sarcoid, the composition may be an injectable formulation for intraperitoneal or intralesional injection.
Typically, the injectable composition is administered in an amount of between about 50 to about 200 mg of sugar free glycoalkaloid composition per kg of tumour. Animal and human studies (as illustrated in the following examples) show that successful treatment of some tumors and cancers may be accomplished with as few as two to four injections. The injection may be given at one, two or three daily intervals, preferably the treatment is given twice, at day 1 and day 3. Treatment by injection may also be given in association with topical administration if desired or considered necessary.
It has also been surprisingly discovered that the therapeutic composition of the present invention may also be used to diagnose skin conditions before such conditions can be detected by visual inspection.
Such diagnosis may be carried out by broadly applying a composition of the present invention to an area of skin to be tested. The composition is left on the skin for a pre-determined period of time. During this time, any abnormal cells are selectively destroyed.
This produces a detectable inflammation of the affected areas which may then be identified and treated.
According to a further broad form of the present invention there is provided a method of diagnosing a skin condition in a mammal, the skin condition being caused by the presence of abnormal cells, wherein the method includes applying an effective amount of a composition or preparation of the present invention to an area of skin to be diagnosed, leaving the composition on the skin for a pre-determined period of time, removing the composition and detecting any change to any areas of skin.
The diagnostic method of the present invention is particularly suitable for diagnosing skin conditions of humans. Typical conditions which may be diagnosed include Keratoses, basal cell carcinomas, squamous cell carcinomas, melanomas or other skin cancers.
A particularly preferred diagnostic composition is a solasodine glycoside mixture having about the same glycoside composition as BEC but without free sugars or the aglycone, solasodine. In trials conducted by the present inventor it has been observed that the normal healthy skin tissue is unaffected by the composition.
This demonstrates the selectivity of glycoalkaloids for abnormal cells.
This method of diagnosis allows skin conditions to be detected and treated at an early stage, typically before the condition produces visible skin lesions.
It should be appreciated that such a method of diagnosis would not be possible with conventional skin treatment compositions which adversely affect all cells.
A further advantage of such specificity is that during application, should the composition be inadvertently applied to a patients' healthy skin, the healthy skin will not be damaged. This does not occur with conventional skin treatment where care must be exercised to avoid contact with healthy skin.
Further, in view of the suprisingly improved efficacy of the present invention in treatment of skin conditions, the diagnosis can be conducted using very low concentrations of solasodine glycosides.
BRIEF DESCRIPTION OF THE DRAWINGS
Figures 1 and 3 and Figures 2 and 4 illustrate HPLC spectra for unwashed and washed BEC respectively.
Figures 5 to 8 illustrate a sarcoid tumor in a horse, before (Figure 5), during (Figures 6 and 7) and after (Figure 8) treatment with a preferred composition of the present invention.
Figures 9 to 12 illustrate the stages in the treatment of a horse with a penile sarcoma before (Figure 9) during (Figures 10 and 11) and after (Figure 12) treatment with a preferred composition of the present invention.
Figures 13 to 15 illustrate stages in the treatment of a human with squamous cell carcinoma before (Figure 13) and during (Figures 14 and 15) treatment.
BEST MODE
The present invention will now be described with reference to the following non-limiting examples.
Example 1 A sugar free solasodine glycoside preparation was prepared according to the following:
50kg Solanum Sodomaeum berries are put through commercial meat mincer (fitted with 1.HP electric motor 1425 rpm) with a sieve size of 3mm.
The slurry is diluted with 3o acetic acid (pH
2.5) (food grade) to a volume of 200L. This semi-solid solution is treated with a Silverson homogenizer for 15 minutes. Mixing is continued for another 4 hours using a SS rod with arms mixer at room temperature at 30 rpm (Flamingo CMG 0.75kw variable speed control meter).
The solution is allowed to stand overnight without mixing. The solution is subsequently filtered through a muslin cloth. The filtrate is then subjected to a flow through centrifuge (3.5HP) at 1455 rpm. The clear filtrate is heated to 50°C in a stainless steel double jacketed bowl. Concentrated ammonia (L R Grade) 5 is added until pH ~ 10. A precipitate is observed. The precipitate is allowed to settle and cool (approx. 24 hrs). The supernatant is carefully decanted. The precipitate is dissolved in 25L 3% aqueous acetic acid.
The solution is centrifuged through flow through 10 centrifuge as above. The supernatant is collected in an SS double jacketed bowl and heated to 50°C with continuous stirring (30 rpm, 30min).
The glycoalkaloids are re-precipitated by the addition of concentrated ammonia solution until pH ~ 10.
15 The solution is allowed to cool and the precipitate is allowed to settle (approx. 24 hrs). The supernatant is carefully decanted and the precipitate is washed with 50L
water and allowed to settle for 24 hrs as before. The supernatant is decanted and this procedure is repeated four times.
The precipitate is finally dissolved in lOL
alcohol at 75°C and filtered whilst hot through Whatman No. 1 filter paper. The supernatant is dried at 50°C.
This yields a fine, semicrystalline powder. The yield is 5058 which is l.Olo.
Any aglycone, solasodine, is removed by washing the extract in chloroform. The solasodine is soluble in the chloroform phase and the sugars are soluble in the aqueous phase. The glycoalkaloids remain insoluble under all these conditions.
Example 2 Cream formulations were prepared from the sugar free solasodine glycoside preparation from Example 1 as follows Percentage Function Composition Active ingredient Solasodine 0.005 % w/w Antineoplast Glycosides (BEC) Other ingredients Cetomacrogol 15.0% w/w Emulsifying agent emulsifying wax White soft paraffin 10.0% w/w Cream base Liguid paraffin 10.0% w/w Cream base Salicylic acid 10.0% w/w Keratolytic Urea 5.0% w/w Keratolytic Propylene glycol 5.0% w/w Emollient Chlorocresol 0.1% w/w Preservative Acetic or lactic qs Solvent acid Purified water qs Solvent/
Cream base Emulsifying wax, white soft paraffin, liquid paraffin, propylene glycol and water were used to provide a cream base of a suitable consistency and viscosity.
Chlorocresol was included in the formulation as a preservative. Salicylic acid and urea were included as keratolytic agents and were considered to be excipients in the cream formulation because their primary function was to enhance the bioavailability of the active ingredient by clearing tissue from around the tumor, thus allowing a higher concentration of the active ingredients to reach the tumor. (The International Pharmaceutical Excipients Council definition of an excipient includes substances which are included in a drug delivery system to protect, support or enhance stability, bioavailability or patient acceptability and to enhance any other attribute of the overall safety and effectiveness of the drug during storage or use).
Acetic or lactic acid was present in the final formulation because a 3°s solution of acetic acid is used as the solvent for the active ingredients during the manufacturing process.
Example 3 White soft paraffin, liquid paraffin and cetomacrogol emulsifying wax were weighed into a sanitized stainless steel container ("Phase A"). This mixture was gently heated on a low burner until the temperature reached 70°C.
Purified water at 70°C, urea, chlorocresol and propylene glycol were added to a suitable stainless steel container and mixed for 2 minutes using a Silverson mixer ("Phase B") .
The melted Phase A was slowed added to Phase B
and thoroughly mixed using a Silverson mixer or follow-through homogenizer. The mixture was allowed to cool to about 50°C and then the salicylic acid was added.
The freshly washed solasodine glycosides were dissolved in acetic acid or lactic acid solution and added to the cream, with mixing to ensure even dispersion. The cream was allowed to cool to room temperature with occasional mixing to ensure an even, smooth texture.
The formulated cream had the following specifications:
Description Smooth, white or slightly off-white cream BEC assay 0.0046 - 0.0054%
Salicylic acid assay 9.5 - 10.50 pH Less than 3 Example 4 The solasodine glycoside preparation from Example 1 and creams from examples 2 and 3 were analyzed for hydrolysis products by MS and HPLC according to the following procedure.
Sample Preparation:
Standard was prepared in 50o CH3CN/Hz0 at lmg/ml and 100ug/ml. Cream was prepared by dissolving 100mg cream in 2m1 methylene chloride, after centrifugation 100u1 of the aqueous phase was removed and made up to lml with methanol.
HPLC Conditions:
A Waters Alliance system was used consisting of a 2690 separations module, and 996 diode .array detector.
A Micromass Waters Platform LCZ mass spectrometer was interfaced and the whole system was controlled by MassLynx chromatography software.
Solvent:
Isocractic analysis was performed with 750 CH3CN/Hz0 Gradients were run from 80% CH3CN to 50 o CH3CN
over 7 minutes Flow Rate was 1.0 or 0.5 ml/min W Detection was from 205nm to 320nm (205nm was extracted for alkaloids and 254 for salicylic acid and chlorocresol) Mass Spec full scans from 400-900 m/z were used for TIC chromatograms and single channels of 869 and 885 were used for quantitation.
Full detailed conditions including cone voltages are attached.
Column High Performance Carbohydrate column (4um) 0.46 *25cm was used.
Results The standard gave three peaks corresponding to Solasonine, Solamargine and an unidentified peak at mass 722. Some smaller peaks were also observed but no indication of mass 414 consistent with the aglycone, hence no obvious hydrolysis of the samples. The cream showed both actives as well as propylene glycol, salicylic acid and chlorocresol. MS results were approximately 1000 times more sensitive than UV.
Example 5 HPLC studies were also conducted on BEC
preparations obtained by conventional extraction procedures (i.e. without any washing steps) and stored for a period of up to about 6 - 8 months. HPLC analysis was conducted on the stored BEC both before and after washing to remove free sugars and solasonine.
Figures 1 to 4 illustrate HPLC spectra of unwashed (Figures 1 and 3) and washed (Figures 2 and 4) BEC respectively.
Compounds marked as I and II had the same elution times as solasonine and solamargine standards.
Figures 1 and 2 show that the unwashed BEC
includes a number of further peaks. By comparison with Figures 3 and 4, it can be seen that these peaks have been removed or significantly decreased upon washing with water and chloroform. These further peaks have been assigned to the various sugar degradation products of BEC.
The compounds represented by peaks I and II
have increased in height relative to the remaining peaks.
It can be seen that BEC undergoes degradation under normal storage conditions. These degradation products may be removed by washing the stored BEC with water and chloroform.
Example 6 A BEC extract was obtained from Solanum Sodomaeum according to conventional extraction procedures. The degradation of the BEC extract was estimated by measuring the change in solamargine and solasonine levels over time. Although degradation of BEC
could also be measured by an increase in sugar levels, in practice HPLC analysis for solamargine and solasonine allowed a more quantative analysis to be conducted and was therefore chosen for this study.
The results are illustrated in the following Table.
Degradation (~) mean +.S.D.
Time/Years Solamargine Solasonine 0.5 4+4 5+5 8+5 10+5 2 12+7 15+8 3 15+8 19+9 5 22+10 23+11 It can be seen that degradation of the solasodine glycosides of the BEC occurs over time. The effectiveness of cream formulations prepared from this BEC was observed to decrease with the time the BEC was stored prior to formulating the cream. This decrease in efficacy resulted in an increase in the duration of treatment required for regression of skin conditions treated by the cream.
It will be appreciated that even after 5 years the relative amounts of free sugars produced by degradation of solamargine and solasonine are present in relatively low amounts. Any inhibition at these low levels could not have been predicted from the observation that a large molar excess of rhamnose in the aforementioned studies. It should also be noted that the decrease in efficacy observed with stored BEC is inconsistent with what would be predicted from the small decrease in concentration of the active agents, solamargine and solasonine.
Example 7 The survival of mice with sarcoma 180 when treated with varying doses of 7mg and 8mg unwashed and washed BEC/kg.
Washed BEC was prepared according to Example 1 and administered immediately after preparation.
Unwashed BEC was prepared in a similar manner but was not washed prior to use. The unwashed crystalline BEC was stored under ambient conditions for about four weeks prior to administration to the mice. By reference to the degradation studies provided in the previous example, the % degradation over the four weeks can be estimated to be between less than 4 to 5% for solamargine and solasonine respectively.
Although, this degree of degradation may be considered to be negligible, it can be seen that there is a significant decrease in efficiency. Thus, BEC should be washed prior to formulation even after storage for even short periods of time (such as about four days).
12 mice with sarcoma 180 were treated with 7mg/kg doses given on consecutive days. The results are shown in the following table.
COMPOUND DOSE NUMBER SURVIVAL ANIMALS
OF DOSES TIME SURVIVED
TREATED
- - - 20.9~5.6 0/12 BEC
Unwashed 7 1 20.96.0 0/12 7 2 29.1+6.6 2/12 7 3 37.516.2 4/12 7 4 42.0+17.1 6/12 BEC
Washed 7 1 25.36.1 0/12 7 2 44 14.2 7/12 7 3 53.010.0 11/12 7 4 56.0 12/12 BEC
Unwashed 8 1 20.9 5.5 0/12 8 2 30.1 15.8 4/12 8 3 48.0 16.2 11/12 8 4 53.0 12.6 11/12 BEC
Washed 8 1 38.3 10.2 7/12 8 2 55.1 6.8 11/12 8 3 56.0 12/12 8 4 56.0 12/12 Each value is the mean ~ S D obtained in groups of twelve tumour - bearing mice treated intraperitoneally 0.5h after tumour implantation (5 x 105 cells/mouse).
a The criterion of survival was taken as 56 days because it was shown that if the treatment was effective against sarcoma 180 for this period, the mice then had a normal life span (approximately 3 years ) .
b Doses given on consecutive days.
Animals surviving after eight weeks.
It can be seen that the % survival for animals treated with the washed BEC was superior when compared with animals treated with an equivalent dose of unwashed BEC. For example, the % survival rate for four doses of unwashed BEC is 50% as compared with 100% for washed BEC.
Example 8 The effect of washed and unwashed BEC or human ovarian cancer cells was compared. The washed and unwashed BEC were prepared as described for Example 8.
Cells (5 x 104) were transferred (200 ~,1/chamber of a microscope slide (Lab Tek Miles Scientific).
Controls received 50 ~1 HIFCS/TCM and experimental chambers 50 ~l of solasodine glycosides (BEC) 1.5 - 3.8 ~M/L, washed and unwashed after 7-h preincubation and incubated for a further 17h and 3-15.3 ~M/12L h preincubation and incubated for a further 3h. Similarly, the cells were treated with the aglycone solasodine 19.4-96.8 ~M/L. The cells were fixed and examined by the Papanicolaou method.
The results are shown in the following table.
BEC unwashed %survival BEC washed %survival ~g/mL ~g/ml Solasodine % survival ~g/ml These results again illustrate the surprisingly superior efficiency of the washed BEC.
Example 9 A patient with no visible lesions on the face had a cream as prepared in Example 2 applied to the skin of the face.
The cream was left for 30 minutes before being washed away. The patient's face was then examined. Areas of redness were noted which were identified as pre-malignant or malignant skin lesions in the very early stages of development. The affected areas of skin were subsequently treated with the same cream.
Example 10 A human patient was diagnosed with an intra epithelial penile tumour. The prognosis was that no treatment was available and that amputation was the only option. The patient commenced treatment with the cream as prepared in Exampl e 2 and was applied to the tumour twice daily. Necrosis of the tumour was observed to occur shortly after treatment commenced. Within six weeks, the patient was observed to be free of the tumour.
Example 11 The use of a preferred composition of the present invention was trialed on solid tumors in animals and humans as follows:
Formulation: A sugar and aglycone free solasodine glycoside preparation which was prepared according to Example 1 in DMSO. DMSO is used for its aprotic characteristics and because when pure is sterile.
Models: Horses, Dogs, Humans.
Lesions: Solid Sarcoids and squamous cell carcinoma (SCC).
Procedure: The approximate weight of the sarcoid or SCC is assessed then 100mg of the sugar and aglycone free solasodine glycosides preparation of Example 1 (100mg/ml DMSO of stock solution) is injected intralesionally to lkg tumor weight. Two days later this procedure is repeated.
Results: At day 2 after the first injection, massive necrosis is observed. Two weeks later ablation of tumor is achieved.
Figures 5, 6, 7 and 8 show an example of a sarcoid tumor in a horse, before, during and after treatment. Figure 5 illustrates the sarcoma before treatment. Figures 6 and 7 show the sarcoma after injection with the above composition. Necrosis of the sarcoma can be seen. Figure 4 shows that the sarcoma has fully regressed after treatment.
Figures 9 to 12 show a further example of the 5 treatment of a horse with a penile tumor before, during and after treatment. Figure 9 shows the horse prior to treatment. The horse was anesthetized and the tumor injected with the above formulation. Figure 10 shows the response of the tumor to the composition. The tumor then 10 separated entirely and fell off as shown in Figure 11.
Figure 12 illustrates the penis after the treatment.
Figures 13, 14 and 15 show an example of the treatment of a human SCC. Figure 13 shows the SCC
located on the patient's scalp. The patient was treated 15 with a single injection and recovery of the SCC shortly after treatment occurred as illustrated in Figures 14 and 15.
In the above examples, it can be seen that a composition of the present invention was successful in 20 the treatment of solid sarcoids in animals and SCC in humans. During treatment, necrosis of the lesion was observed to begin almost immediately after injection.
It was also observed that similar treatment with BEC which contains free sugars was less effective 25 than the inventive composition. Treatment with BEC
required much higher dosages before any effect was observed.
The dosages of the compositions in the above examples is 100mg of solasodine glycosides per lkg tumor.
A typical tumor is 1008 such that a typical injection contains lOmg solasodine glycosides. This dose for a 500kg horse corresponds to 0.02mg/kg body weight.
It can be seen that a therapeutic composition of the present invention provides a suprising and unexpected improvement in efficacy of glycoalkaloids in the treatment of cancers and tumors. This increase in efficacy allows disease conditions to be treated with dosages which are well below the threshold level of toxicity for normal cells. This is advantageous for the patient and also allows the inventive compositions to be used as diagnostic tools. Still further, the improved efficacy enables the duration of treatments to be reduced and total dosages to be decreased. This is advantageous for the overall safety and comfort of the patient and also provides a superior treatment regime in terms of cost effectiveness.
Throughout the specification (including claims if present) unless the context requires otherwise, the word " comprise" or variations such as " comprising"
will be understood to imply the inclusion of a stated integer or group of integers but not the exclusion of any other integer or group of integers.
It will be appreciated that modifications and changes may be made to the embodiments described therein without departing from the spirit and scope of the invention as herein described.
water and allowed to settle for 24 hrs as before. The supernatant is decanted and this procedure is repeated four times.
The precipitate is finally dissolved in lOL
alcohol at 75°C and filtered whilst hot through Whatman No. 1 filter paper. The supernatant is dried at 50°C.
This yields a fine, semicrystalline powder. The yield is 5058 which is l.Olo.
Any aglycone, solasodine, is removed by washing the extract in chloroform. The solasodine is soluble in the chloroform phase and the sugars are soluble in the aqueous phase. The glycoalkaloids remain insoluble under all these conditions.
Example 2 Cream formulations were prepared from the sugar free solasodine glycoside preparation from Example 1 as follows Percentage Function Composition Active ingredient Solasodine 0.005 % w/w Antineoplast Glycosides (BEC) Other ingredients Cetomacrogol 15.0% w/w Emulsifying agent emulsifying wax White soft paraffin 10.0% w/w Cream base Liguid paraffin 10.0% w/w Cream base Salicylic acid 10.0% w/w Keratolytic Urea 5.0% w/w Keratolytic Propylene glycol 5.0% w/w Emollient Chlorocresol 0.1% w/w Preservative Acetic or lactic qs Solvent acid Purified water qs Solvent/
Cream base Emulsifying wax, white soft paraffin, liquid paraffin, propylene glycol and water were used to provide a cream base of a suitable consistency and viscosity.
Chlorocresol was included in the formulation as a preservative. Salicylic acid and urea were included as keratolytic agents and were considered to be excipients in the cream formulation because their primary function was to enhance the bioavailability of the active ingredient by clearing tissue from around the tumor, thus allowing a higher concentration of the active ingredients to reach the tumor. (The International Pharmaceutical Excipients Council definition of an excipient includes substances which are included in a drug delivery system to protect, support or enhance stability, bioavailability or patient acceptability and to enhance any other attribute of the overall safety and effectiveness of the drug during storage or use).
Acetic or lactic acid was present in the final formulation because a 3°s solution of acetic acid is used as the solvent for the active ingredients during the manufacturing process.
Example 3 White soft paraffin, liquid paraffin and cetomacrogol emulsifying wax were weighed into a sanitized stainless steel container ("Phase A"). This mixture was gently heated on a low burner until the temperature reached 70°C.
Purified water at 70°C, urea, chlorocresol and propylene glycol were added to a suitable stainless steel container and mixed for 2 minutes using a Silverson mixer ("Phase B") .
The melted Phase A was slowed added to Phase B
and thoroughly mixed using a Silverson mixer or follow-through homogenizer. The mixture was allowed to cool to about 50°C and then the salicylic acid was added.
The freshly washed solasodine glycosides were dissolved in acetic acid or lactic acid solution and added to the cream, with mixing to ensure even dispersion. The cream was allowed to cool to room temperature with occasional mixing to ensure an even, smooth texture.
The formulated cream had the following specifications:
Description Smooth, white or slightly off-white cream BEC assay 0.0046 - 0.0054%
Salicylic acid assay 9.5 - 10.50 pH Less than 3 Example 4 The solasodine glycoside preparation from Example 1 and creams from examples 2 and 3 were analyzed for hydrolysis products by MS and HPLC according to the following procedure.
Sample Preparation:
Standard was prepared in 50o CH3CN/Hz0 at lmg/ml and 100ug/ml. Cream was prepared by dissolving 100mg cream in 2m1 methylene chloride, after centrifugation 100u1 of the aqueous phase was removed and made up to lml with methanol.
HPLC Conditions:
A Waters Alliance system was used consisting of a 2690 separations module, and 996 diode .array detector.
A Micromass Waters Platform LCZ mass spectrometer was interfaced and the whole system was controlled by MassLynx chromatography software.
Solvent:
Isocractic analysis was performed with 750 CH3CN/Hz0 Gradients were run from 80% CH3CN to 50 o CH3CN
over 7 minutes Flow Rate was 1.0 or 0.5 ml/min W Detection was from 205nm to 320nm (205nm was extracted for alkaloids and 254 for salicylic acid and chlorocresol) Mass Spec full scans from 400-900 m/z were used for TIC chromatograms and single channels of 869 and 885 were used for quantitation.
Full detailed conditions including cone voltages are attached.
Column High Performance Carbohydrate column (4um) 0.46 *25cm was used.
Results The standard gave three peaks corresponding to Solasonine, Solamargine and an unidentified peak at mass 722. Some smaller peaks were also observed but no indication of mass 414 consistent with the aglycone, hence no obvious hydrolysis of the samples. The cream showed both actives as well as propylene glycol, salicylic acid and chlorocresol. MS results were approximately 1000 times more sensitive than UV.
Example 5 HPLC studies were also conducted on BEC
preparations obtained by conventional extraction procedures (i.e. without any washing steps) and stored for a period of up to about 6 - 8 months. HPLC analysis was conducted on the stored BEC both before and after washing to remove free sugars and solasonine.
Figures 1 to 4 illustrate HPLC spectra of unwashed (Figures 1 and 3) and washed (Figures 2 and 4) BEC respectively.
Compounds marked as I and II had the same elution times as solasonine and solamargine standards.
Figures 1 and 2 show that the unwashed BEC
includes a number of further peaks. By comparison with Figures 3 and 4, it can be seen that these peaks have been removed or significantly decreased upon washing with water and chloroform. These further peaks have been assigned to the various sugar degradation products of BEC.
The compounds represented by peaks I and II
have increased in height relative to the remaining peaks.
It can be seen that BEC undergoes degradation under normal storage conditions. These degradation products may be removed by washing the stored BEC with water and chloroform.
Example 6 A BEC extract was obtained from Solanum Sodomaeum according to conventional extraction procedures. The degradation of the BEC extract was estimated by measuring the change in solamargine and solasonine levels over time. Although degradation of BEC
could also be measured by an increase in sugar levels, in practice HPLC analysis for solamargine and solasonine allowed a more quantative analysis to be conducted and was therefore chosen for this study.
The results are illustrated in the following Table.
Degradation (~) mean +.S.D.
Time/Years Solamargine Solasonine 0.5 4+4 5+5 8+5 10+5 2 12+7 15+8 3 15+8 19+9 5 22+10 23+11 It can be seen that degradation of the solasodine glycosides of the BEC occurs over time. The effectiveness of cream formulations prepared from this BEC was observed to decrease with the time the BEC was stored prior to formulating the cream. This decrease in efficacy resulted in an increase in the duration of treatment required for regression of skin conditions treated by the cream.
It will be appreciated that even after 5 years the relative amounts of free sugars produced by degradation of solamargine and solasonine are present in relatively low amounts. Any inhibition at these low levels could not have been predicted from the observation that a large molar excess of rhamnose in the aforementioned studies. It should also be noted that the decrease in efficacy observed with stored BEC is inconsistent with what would be predicted from the small decrease in concentration of the active agents, solamargine and solasonine.
Example 7 The survival of mice with sarcoma 180 when treated with varying doses of 7mg and 8mg unwashed and washed BEC/kg.
Washed BEC was prepared according to Example 1 and administered immediately after preparation.
Unwashed BEC was prepared in a similar manner but was not washed prior to use. The unwashed crystalline BEC was stored under ambient conditions for about four weeks prior to administration to the mice. By reference to the degradation studies provided in the previous example, the % degradation over the four weeks can be estimated to be between less than 4 to 5% for solamargine and solasonine respectively.
Although, this degree of degradation may be considered to be negligible, it can be seen that there is a significant decrease in efficiency. Thus, BEC should be washed prior to formulation even after storage for even short periods of time (such as about four days).
12 mice with sarcoma 180 were treated with 7mg/kg doses given on consecutive days. The results are shown in the following table.
COMPOUND DOSE NUMBER SURVIVAL ANIMALS
OF DOSES TIME SURVIVED
TREATED
- - - 20.9~5.6 0/12 BEC
Unwashed 7 1 20.96.0 0/12 7 2 29.1+6.6 2/12 7 3 37.516.2 4/12 7 4 42.0+17.1 6/12 BEC
Washed 7 1 25.36.1 0/12 7 2 44 14.2 7/12 7 3 53.010.0 11/12 7 4 56.0 12/12 BEC
Unwashed 8 1 20.9 5.5 0/12 8 2 30.1 15.8 4/12 8 3 48.0 16.2 11/12 8 4 53.0 12.6 11/12 BEC
Washed 8 1 38.3 10.2 7/12 8 2 55.1 6.8 11/12 8 3 56.0 12/12 8 4 56.0 12/12 Each value is the mean ~ S D obtained in groups of twelve tumour - bearing mice treated intraperitoneally 0.5h after tumour implantation (5 x 105 cells/mouse).
a The criterion of survival was taken as 56 days because it was shown that if the treatment was effective against sarcoma 180 for this period, the mice then had a normal life span (approximately 3 years ) .
b Doses given on consecutive days.
Animals surviving after eight weeks.
It can be seen that the % survival for animals treated with the washed BEC was superior when compared with animals treated with an equivalent dose of unwashed BEC. For example, the % survival rate for four doses of unwashed BEC is 50% as compared with 100% for washed BEC.
Example 8 The effect of washed and unwashed BEC or human ovarian cancer cells was compared. The washed and unwashed BEC were prepared as described for Example 8.
Cells (5 x 104) were transferred (200 ~,1/chamber of a microscope slide (Lab Tek Miles Scientific).
Controls received 50 ~1 HIFCS/TCM and experimental chambers 50 ~l of solasodine glycosides (BEC) 1.5 - 3.8 ~M/L, washed and unwashed after 7-h preincubation and incubated for a further 17h and 3-15.3 ~M/12L h preincubation and incubated for a further 3h. Similarly, the cells were treated with the aglycone solasodine 19.4-96.8 ~M/L. The cells were fixed and examined by the Papanicolaou method.
The results are shown in the following table.
BEC unwashed %survival BEC washed %survival ~g/mL ~g/ml Solasodine % survival ~g/ml These results again illustrate the surprisingly superior efficiency of the washed BEC.
Example 9 A patient with no visible lesions on the face had a cream as prepared in Example 2 applied to the skin of the face.
The cream was left for 30 minutes before being washed away. The patient's face was then examined. Areas of redness were noted which were identified as pre-malignant or malignant skin lesions in the very early stages of development. The affected areas of skin were subsequently treated with the same cream.
Example 10 A human patient was diagnosed with an intra epithelial penile tumour. The prognosis was that no treatment was available and that amputation was the only option. The patient commenced treatment with the cream as prepared in Exampl e 2 and was applied to the tumour twice daily. Necrosis of the tumour was observed to occur shortly after treatment commenced. Within six weeks, the patient was observed to be free of the tumour.
Example 11 The use of a preferred composition of the present invention was trialed on solid tumors in animals and humans as follows:
Formulation: A sugar and aglycone free solasodine glycoside preparation which was prepared according to Example 1 in DMSO. DMSO is used for its aprotic characteristics and because when pure is sterile.
Models: Horses, Dogs, Humans.
Lesions: Solid Sarcoids and squamous cell carcinoma (SCC).
Procedure: The approximate weight of the sarcoid or SCC is assessed then 100mg of the sugar and aglycone free solasodine glycosides preparation of Example 1 (100mg/ml DMSO of stock solution) is injected intralesionally to lkg tumor weight. Two days later this procedure is repeated.
Results: At day 2 after the first injection, massive necrosis is observed. Two weeks later ablation of tumor is achieved.
Figures 5, 6, 7 and 8 show an example of a sarcoid tumor in a horse, before, during and after treatment. Figure 5 illustrates the sarcoma before treatment. Figures 6 and 7 show the sarcoma after injection with the above composition. Necrosis of the sarcoma can be seen. Figure 4 shows that the sarcoma has fully regressed after treatment.
Figures 9 to 12 show a further example of the 5 treatment of a horse with a penile tumor before, during and after treatment. Figure 9 shows the horse prior to treatment. The horse was anesthetized and the tumor injected with the above formulation. Figure 10 shows the response of the tumor to the composition. The tumor then 10 separated entirely and fell off as shown in Figure 11.
Figure 12 illustrates the penis after the treatment.
Figures 13, 14 and 15 show an example of the treatment of a human SCC. Figure 13 shows the SCC
located on the patient's scalp. The patient was treated 15 with a single injection and recovery of the SCC shortly after treatment occurred as illustrated in Figures 14 and 15.
In the above examples, it can be seen that a composition of the present invention was successful in 20 the treatment of solid sarcoids in animals and SCC in humans. During treatment, necrosis of the lesion was observed to begin almost immediately after injection.
It was also observed that similar treatment with BEC which contains free sugars was less effective 25 than the inventive composition. Treatment with BEC
required much higher dosages before any effect was observed.
The dosages of the compositions in the above examples is 100mg of solasodine glycosides per lkg tumor.
A typical tumor is 1008 such that a typical injection contains lOmg solasodine glycosides. This dose for a 500kg horse corresponds to 0.02mg/kg body weight.
It can be seen that a therapeutic composition of the present invention provides a suprising and unexpected improvement in efficacy of glycoalkaloids in the treatment of cancers and tumors. This increase in efficacy allows disease conditions to be treated with dosages which are well below the threshold level of toxicity for normal cells. This is advantageous for the patient and also allows the inventive compositions to be used as diagnostic tools. Still further, the improved efficacy enables the duration of treatments to be reduced and total dosages to be decreased. This is advantageous for the overall safety and comfort of the patient and also provides a superior treatment regime in terms of cost effectiveness.
Throughout the specification (including claims if present) unless the context requires otherwise, the word " comprise" or variations such as " comprising"
will be understood to imply the inclusion of a stated integer or group of integers but not the exclusion of any other integer or group of integers.
It will be appreciated that modifications and changes may be made to the embodiments described therein without departing from the spirit and scope of the invention as herein described.
Claims (29)
1. A medicinal composition comprising at least one compound which can interact with a target cell, the at least one compound being a glycoalkaloid of the general formula I:
wherein:
either one of the dotted lines represents a double bond, and the other a single bond, or both represent single bonds;
A: represents a radical selected from the following radicals of general formulae (II) to (V):
each of R1 is a radical separately selected from the group consisting of hydrogen, amino, oxo and OR4; each of R2 is a radical separately selected from the group consisting of hydrogen, amino and OR4; each of R3 is a radical separately selected from the group consisting of hydrogen, alkyl and R4-alkylene; each of R4 is a radical separately selected from the group consisting of hydrogen, carbohydrate and a carbohydrate derivative; "X" is a radical selected from the group comprising -CH2-, -O- and -NH2-;
wherein the compound includes at least one R4 group in which R4 is a carbohydrate or a derivative thereof;
together with a pharmaceutically acceptable carrier, adjuvant, excipient and/or diluent, wherein the composition is essentially without free sugars of the type which inhibit the interaction between the at least one glycoalkaloid and a target cell.
wherein:
either one of the dotted lines represents a double bond, and the other a single bond, or both represent single bonds;
A: represents a radical selected from the following radicals of general formulae (II) to (V):
each of R1 is a radical separately selected from the group consisting of hydrogen, amino, oxo and OR4; each of R2 is a radical separately selected from the group consisting of hydrogen, amino and OR4; each of R3 is a radical separately selected from the group consisting of hydrogen, alkyl and R4-alkylene; each of R4 is a radical separately selected from the group consisting of hydrogen, carbohydrate and a carbohydrate derivative; "X" is a radical selected from the group comprising -CH2-, -O- and -NH2-;
wherein the compound includes at least one R4 group in which R4 is a carbohydrate or a derivative thereof;
together with a pharmaceutically acceptable carrier, adjuvant, excipient and/or diluent, wherein the composition is essentially without free sugars of the type which inhibit the interaction between the at least one glycoalkaloid and a target cell.
2. The composition of claim 1, wherein R4 is selected from the group consisting of glyceric aldehyde; glycerose;
erythrose; threose; ribose; arabinose; xylose; lyxose;
altrose; allose; gulose; mannose; glucose; idose; galactose;
talose; rhamnose; dihydroxyactone; erythrulose; ribulose;
xylulose; psicose; fructose; sorbose; tagatose; and other hexoses (C6H12O6) heptoses (C7H14O7); octoses (C8H16O8);
nanoses (C9H18O9); decoses (C10H20O10); deoxysugars with branched chains (eg. apiose, hamamelose, streptose, cordycepose, mycarose and cladinose); compounds wherein the aldehyde, ketone or hydroxyl groups have been substituted (eg. N-acetyl, acetyl, methyl, replacement of CH2OH); sugar alcohols; sugar acids; benzimidazoles; the enol salts of the carbohydrates; saccharinic acids; sugar phosphates.
erythrose; threose; ribose; arabinose; xylose; lyxose;
altrose; allose; gulose; mannose; glucose; idose; galactose;
talose; rhamnose; dihydroxyactone; erythrulose; ribulose;
xylulose; psicose; fructose; sorbose; tagatose; and other hexoses (C6H12O6) heptoses (C7H14O7); octoses (C8H16O8);
nanoses (C9H18O9); decoses (C10H20O10); deoxysugars with branched chains (eg. apiose, hamamelose, streptose, cordycepose, mycarose and cladinose); compounds wherein the aldehyde, ketone or hydroxyl groups have been substituted (eg. N-acetyl, acetyl, methyl, replacement of CH2OH); sugar alcohols; sugar acids; benzimidazoles; the enol salts of the carbohydrates; saccharinic acids; sugar phosphates.
3. The composition of claim 1, wherein the at least one glycoalkaloid is selected from the group consisting of solasonine, solamargine, and tomatine.
4. The composition of claim 1, wherein the at least one glycoalkaloid has been extracted from a plant source.
5. The composition of claim 4, wherein the plant source is from the Solanum genus.
6. The composition of claim 5, wherein the composition is a BEC mixture of solasodine glycosides.
7. The composition of claim 1, wherein the free sugar is rhammose, or a disaccharide, trisaccharide, oligesaccharide or polysaccharide having rhannose as a sugar moiety thereof.
8. The composition of claim 1 which is essentially free of any aglycone degradation product of the glycoalkaloid.
9. The composition of claim 1 in a form suitable for topical administration.
10. The composition of claim 9, which includes between about 0.001% to about 5 wt% of the at least one glycoalkaloid.
11. The composition of claim 1, which is in a form suitable for administration by injection.
12. The composition of claim 11, which includes a liquid carrier selected from the group consisting of DMSO, acetic acid and lactic acid.
13. The composition of claim 1, which includes a stablizing agent for stabilizing the at least one glycoalkaloid.
14. A method of preparing a glycoalkaloid preparation which comprises at least one glycoalkaloid of the general formula I:
wherein:
either one of the dotted lines represents a double bond, and the other a single bond, or both represent single bonds;
A: represents a radical selected from the following radicals of general formulae (II) to (V):
each of R1 is a radical separately selected from the group consisting of hydrogen, amino, oxo and OR4; each of R2 is a radical separately selected from the group consisting of hydrogen, amino and OR4; each of R3 is a radical separately selected from the group consisting of hydrogen, alkyl and R4-alkylene; each of R4 is a radical separately selected from the group consisting of hydrogen, carbohydrate and a carbohydrate derivative;" X"
is a radical selected from the group comprising -CH2-, -O-and -NH2-;
wherein the compound includes at least one R4 group in which R4 is a carbohydrate or a derivative thereof;
the method including extracting the at least one glycoalkaloid from a suitable plant material to form an extract and removing essentially all free sugars from the extract.
wherein:
either one of the dotted lines represents a double bond, and the other a single bond, or both represent single bonds;
A: represents a radical selected from the following radicals of general formulae (II) to (V):
each of R1 is a radical separately selected from the group consisting of hydrogen, amino, oxo and OR4; each of R2 is a radical separately selected from the group consisting of hydrogen, amino and OR4; each of R3 is a radical separately selected from the group consisting of hydrogen, alkyl and R4-alkylene; each of R4 is a radical separately selected from the group consisting of hydrogen, carbohydrate and a carbohydrate derivative;" X"
is a radical selected from the group comprising -CH2-, -O-and -NH2-;
wherein the compound includes at least one R4 group in which R4 is a carbohydrate or a derivative thereof;
the method including extracting the at least one glycoalkaloid from a suitable plant material to form an extract and removing essentially all free sugars from the extract.
15. The method of claim 14, wherein the plant material is from the Solanum genus.
16. A method of preparing the composition of claim 1, including obtaining a glycoalkaloid preparation which comprises at least one glycoalkaloid according to general formula I and treating the preparation to remove essentially all of any free sugars from the preparation prior to addition of a pharmaceutically acceptable carrier, adjuvant, excipient and/or diluent.
17. The method of claim 15 wherein the preparation is further treated to remove any aglycone therefrom.
18. The method of claim 16, wherein the preparation is washed with an aqueous solvent.
19. The method of claim 16, wherein the glycoalkaloid preparation is extracted from a plant source.
20. The method of claim 18, wherein the plant source is from the Solanum genus.
21. The method of claim 18, wherein the glycoalkaloid preparation is a BEC mixture of solasodine glycosides.
22. The method of claim 18, wherein a time period of at least about 7 days has elapsed between the extraction and free sugar removal steps.
23. A method for the treatment or control of cancer, contraception, termination of pathogenic organisms and removal of abnormal cellular growth in a mammal requiring such treatment, the method comprising administering to said mammal an effective amount of the medicinal composition of claim 1.
24. A method for the treatment or control of cancers or tumours in a mammal, the method comprising injecting into or about the cancer or tumour a anticarcinogenically effective amount of the composition of claim 11.
25. The method of claim 23, wherein the composition is injected at intervals of one, two or three days.
26. The method of claim 24, wherein the amount of glycoalkaloid injected is between about 50 to about 200 mg per kg of the cancer or tumour.
27. A method of treating a skin lesion of a mammal, the method comprising applying to the lesion an effective amount of the composition of claim 9.
28. The method of claim 27, wherein said lesion is selected from the group consisting of keratoses, basal cell carcinomas, squamous cell carcinomas, melanomas and intra epethelial tumours.
29. A method of diagnosing a skin condition in a mammal, the skin condition being caused by abnormal cells, wherein the method includes applying an effective amount of the composition of claim 9 to an area of skin to be diagnosed, leaving the composition on the skin for a predetermined period of time, removing the composition and detecting any change to the area of skin.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AUPP9686A AUPP968699A0 (en) | 1999-04-09 | 1999-04-09 | Therapeutic compositions and method for their preparation |
AUPP9686 | 1999-04-09 | ||
PCT/AU2000/000300 WO2000061153A1 (en) | 1999-04-09 | 2000-04-10 | Medicinal compositions and their method of preparation |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2369272A1 true CA2369272A1 (en) | 2000-10-19 |
Family
ID=3813885
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002369272A Abandoned CA2369272A1 (en) | 1999-04-09 | 2000-04-10 | Medicinal compositions and their method of preparation |
Country Status (4)
Country | Link |
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EP (1) | EP1181022A4 (en) |
AU (1) | AUPP968699A0 (en) |
CA (1) | CA2369272A1 (en) |
WO (1) | WO2000061153A1 (en) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9923076D0 (en) | 1999-09-29 | 1999-12-01 | Phytopharm Plc | Sapogenin derivatives and their use |
ATE312118T1 (en) * | 2001-09-28 | 2005-12-15 | Glycomed Sciences Ltd | SOLVENT EXTRACTION METHOD |
WO2003030915A1 (en) * | 2001-10-09 | 2003-04-17 | Glycomed Sciences Limited | Use of solamargine for treating skin tumors |
AU2002350513A1 (en) * | 2001-10-09 | 2003-04-22 | Glycomed Sciences Limited | Use of solasonine for the treatment of skin tumors |
AUPS326302A0 (en) * | 2002-06-28 | 2002-07-18 | Solbec Pharmaceuticals Limited | Method for the separation of solamargine and solasonine |
US6984725B2 (en) | 2002-06-28 | 2006-01-10 | Solbec Pharmaceuticals Ltd. | Method for the separation of triglycoalkaloids |
AU2003900194A0 (en) * | 2003-01-15 | 2003-01-30 | Solbec Pharmaceuticals Limited | Methods of modulating il-6 |
TWI300352B (en) * | 2003-06-05 | 2008-09-01 | G & E Herbal Biotechnology Co Ltd | Water soluble extract from plant of solanum genus and the preparation process thereof, and pharmaceutical composition containing the water soluble extract |
ES2341232T3 (en) * | 2003-08-22 | 2010-06-17 | G & E HERBAL BIOTECHNOLOGY CO., LTD. | SOLUBLE WATER EXTRACT FROM A SOLANUM GENDER PLANT AND PREPARATION PROCEDURE OF THE SAME, AND PHARMACEUTICAL COMPOSITION CONTAINING THE WATER SOLUBLE EXTRACT. |
US20080182801A1 (en) | 2003-12-22 | 2008-07-31 | Btg International Limited | Core 2 glcnac-t inhibitors |
GB0513881D0 (en) | 2005-07-06 | 2005-08-10 | Btg Int Ltd | Core 2 GLCNAC-T Inhibitors III |
GB0329667D0 (en) | 2003-12-22 | 2004-01-28 | King S College London | Core 2 GlcNAc-T inhibitor |
WO2006092017A1 (en) * | 2005-03-02 | 2006-09-08 | Solbec Pharmaceuticals Limited | Glycoalkaloid and tlr agonist combinations and various uses thereof |
GB0513888D0 (en) | 2005-07-06 | 2005-08-10 | Btg Int Ltd | Core 2 GLCNAC-T Inhibitors II |
CA2622286A1 (en) * | 2005-09-27 | 2007-04-05 | University Of Kentucky Research Foundation | Anti-inflammatory composition comprising berry extract |
TWI414304B (en) * | 2011-05-04 | 2013-11-11 | G & E Herbal Biotechnology Co Ltd | Treatment of wart with a water soluble extract from plant of solanum genus |
TWI476012B (en) | 2011-05-12 | 2015-03-11 | G & E Herbal Biotechnology Co Ltd | Treatment and/or prevention of inflammation and cutaneous photodamage and photoprotection of the skin with a water soluble extract from plant of solanum genus |
EP3520793B1 (en) * | 2011-06-06 | 2023-03-29 | University of Iowa Research Foundation | Substances and compostions for use in methods of inhibiting muscle atrophy |
CN105192820A (en) * | 2015-08-20 | 2015-12-30 | 泰山医学院 | Blood-fat-reducing cordyceps militaris polysaccharide beverage and preparing method thereof |
CA3016729A1 (en) * | 2016-03-03 | 2017-09-08 | Bill Elliot Cham | Glycoalkaloid combinations and various uses thereof |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
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AU540812B2 (en) * | 1979-05-02 | 1984-12-06 | Aruba Qld Pty. Ltd. | Steroid alkaloids |
DE69131861T2 (en) * | 1990-01-18 | 2000-05-18 | Cura Nominees Pty Ltd | GLYCOAL CALALID |
-
1999
- 1999-04-09 AU AUPP9686A patent/AUPP968699A0/en not_active Abandoned
-
2000
- 2000-04-10 CA CA002369272A patent/CA2369272A1/en not_active Abandoned
- 2000-04-10 EP EP00913972A patent/EP1181022A4/en not_active Withdrawn
- 2000-04-10 WO PCT/AU2000/000300 patent/WO2000061153A1/en active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
EP1181022A4 (en) | 2004-02-11 |
EP1181022A1 (en) | 2002-02-27 |
WO2000061153A1 (en) | 2000-10-19 |
AUPP968699A0 (en) | 1999-05-06 |
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