CA2214800C - Chambre d'electroporation en flux et procede associe - Google Patents
Chambre d'electroporation en flux et procede associe Download PDFInfo
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- CA2214800C CA2214800C CA002214800A CA2214800A CA2214800C CA 2214800 C CA2214800 C CA 2214800C CA 002214800 A CA002214800 A CA 002214800A CA 2214800 A CA2214800 A CA 2214800A CA 2214800 C CA2214800 C CA 2214800C
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- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
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- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3621—Extra-corporeal blood circuits
- A61M1/3623—Means for actively controlling temperature of blood
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- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3692—Washing or rinsing blood or blood constituents
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- G—PHYSICS
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- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
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- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
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- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/04—Liquids
- A61M2202/0413—Blood
- A61M2202/0429—Red blood cells; Erythrocytes
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- A—HUMAN NECESSITIES
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- A61M2205/3306—Optical measuring means
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- A61M2205/00—General characteristics of the apparatus
- A61M2205/33—Controlling, regulating or measuring
- A61M2205/3379—Masses, volumes, levels of fluids in reservoirs, flow rates
- A61M2205/3382—Upper level detectors
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- A61M2205/00—General characteristics of the apparatus
- A61M2205/36—General characteristics of the apparatus related to heating or cooling
- A61M2205/3606—General characteristics of the apparatus related to heating or cooling cooled
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- A61M2205/00—General characteristics of the apparatus
- A61M2205/36—General characteristics of the apparatus related to heating or cooling
- A61M2205/3673—General characteristics of the apparatus related to heating or cooling thermo-electric, e.g. Peltier effect, thermocouples, semi-conductors
Landscapes
- Health & Medical Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
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- Hematology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Biotechnology (AREA)
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- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sustainable Development (AREA)
- Cell Biology (AREA)
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- General Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Automation & Control Theory (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Electrotherapy Devices (AREA)
- External Artificial Organs (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Glass Compositions (AREA)
- Physical Deposition Of Substances That Are Components Of Semiconductor Devices (AREA)
- Apparatuses And Processes For Manufacturing Resistors (AREA)
Abstract
Cette invention se rapporte à un procédé et à un appareil qui permettent d'encapsuler des substances biologiquement actives dans des globules rouges du sang et qui se caractérisent par un système d'électroporation autonome à flux continu éventuellement automatisé (5), qui permet de prélever du sang d'un patient, de séparer les globules rouges et, éventuellement, de recombiner le plasma et les globules rouges modifiés, afin de produire du sang ayant des caractéristiques biologiques modifiées. Cette invention est particulièrement adaptée pour encapsuler des effecteurs allostériques d'hémoglobine, afin de réduire l'affinité des hérythrocytes pour l'oxygène et afin d'améliorer la libération de l'oxygène contenue dans les hérythrocytes de tissus. Le sang est introduit dans le système (5) par une entrée (11) et mélangé à un anticoagulant (27). Le mélange de sang et d'anticoagulant est ensuite amené à passer à travers un filtre (18), jusqu'à atteindre un bol (44) de séparation et de lavage du sang. Le plasma et les globules blancs sont introduits dans le réservoir de plasma (89), tandis que les globules rouges sont retenus dans le bol de lavage (44). Les globules rouges séparés sont ensuite extraits par pompage et mélangés au niveau d'une jonction (67) avec une solution d'hexaphosphate d'inositol (HPI) provenant d'un réservoir (50). Ce mélange est refroidi par un serpentin de refroidissement (68) et ensuite pompé dans une chambre d'électroporation (72) pour y être traité.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002363548A CA2363548A1 (fr) | 1995-03-10 | 1996-03-11 | Chambre d'electroporation en flux et procede associe |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/402,145 US5720921A (en) | 1995-03-10 | 1995-03-10 | Flow electroporation chamber and method |
| US490695P | 1995-10-06 | 1995-10-06 | |
| US08/402,145 | 1995-10-06 | ||
| US60/004,906 | 1995-10-06 | ||
| PCT/US1996/003211 WO1996028199A1 (fr) | 1995-03-10 | 1996-03-11 | Chambre d'electroporation en flux et procede associe |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002363548A Division CA2363548A1 (fr) | 1995-03-10 | 1996-03-11 | Chambre d'electroporation en flux et procede associe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2214800A1 CA2214800A1 (fr) | 1996-09-19 |
| CA2214800C true CA2214800C (fr) | 2002-01-22 |
Family
ID=26673634
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002214800A Expired - Lifetime CA2214800C (fr) | 1995-03-10 | 1996-03-11 | Chambre d'electroporation en flux et procede associe |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0814855A4 (fr) |
| JP (2) | JP3859709B2 (fr) |
| KR (1) | KR19980702848A (fr) |
| CN (1) | CN1195997A (fr) |
| AU (1) | AU5305096A (fr) |
| CA (1) | CA2214800C (fr) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6773669B1 (en) | 1995-03-10 | 2004-08-10 | Maxcyte, Inc. | Flow electroporation chamber and method |
| JP5015379B2 (ja) * | 1999-03-17 | 2012-08-29 | ヘモネティクス・コーポレーション | 血液を処理するための装置及び方法 |
| US7029916B2 (en) | 2001-02-21 | 2006-04-18 | Maxcyte, Inc. | Apparatus and method for flow electroporation of biological samples |
| WO2003018751A2 (fr) | 2001-08-22 | 2003-03-06 | Maxcyte, Inc. | Appareil et procede d'electroporation d'echantillons biologiques |
| US7601268B2 (en) * | 2003-05-27 | 2009-10-13 | Haemonetics Corporation | Continuous blood filtration and method of use |
| CA2565316C (fr) | 2004-05-12 | 2017-05-30 | Maxcyte, Inc. | Procedes et dispositifs associes a une chambre d'electroporation par debit controle |
| ZA200610508B (en) * | 2004-06-12 | 2009-01-28 | Digital Bio Technology Co Ltd | Electroporator having an elongated hollow member |
| AU2005257583A1 (en) * | 2004-06-12 | 2006-01-05 | Digital Bio Technology Co., Ltd. | Electroporator having an elongated hollow member |
| KR101084528B1 (ko) * | 2008-04-15 | 2011-11-18 | 인비트로겐 싱가포르 피티이. 엘티디. | 전기천공 장치용 파이펫 팁 |
| CN101857836B (zh) * | 2009-11-10 | 2013-06-12 | 昆山文曲生物微***有限公司 | 一种流式电穿孔装置及*** |
| EP2338356A1 (fr) * | 2009-12-23 | 2011-06-29 | Südzucker Aktiengesellschaft Mannheim/Ochsenfurt | Système de réacteur pour électroporation |
| GB201012521D0 (en) * | 2010-07-27 | 2010-09-08 | Univ Strathclyde | Integrated perfusion system |
| CN101928666B (zh) * | 2010-07-30 | 2013-04-03 | 北京大学 | 一种流式电穿孔装置及*** |
| US10368937B2 (en) * | 2013-03-13 | 2019-08-06 | Nuvaira, Inc. | Fluid delivery system and method for treatment |
| US10316335B2 (en) | 2013-03-15 | 2019-06-11 | Maxcyte, Inc. | Methods and compositions for generating stable transfected cells |
| EP3277368B1 (fr) * | 2015-03-31 | 2020-05-20 | OncoSec Medical Incorporated | Systèmes pour une électroporation ameliorée basée sur mésures effectuées sur le tissu |
| CA2982759A1 (fr) | 2015-04-13 | 2016-10-20 | Maxcyte, Inc. | Procedes et compositions permettant de modifier l'adn genomique |
| WO2016183032A1 (fr) * | 2015-05-08 | 2016-11-17 | Biomagnetic Solutions Llc | Appareil et procédé de séparation de cellules immunomagnétique |
| WO2017176253A1 (fr) * | 2016-04-05 | 2017-10-12 | General Electiric Comapny | Composition de plaquettes activées ayant un taux de facteur de croissance ajustable |
| CA3062611A1 (fr) | 2017-05-14 | 2018-11-22 | Paragate Medical Ltd. | Systeme implantable d'extraction de fluide |
| WO2019068022A1 (fr) * | 2017-09-30 | 2019-04-04 | Inscripta, Inc. | Instrumentation d'électroporation à flux continu |
| CN108795750A (zh) * | 2018-05-28 | 2018-11-13 | 深圳先进技术研究院 | 一种带冷却功能的电穿孔装置 |
| CA3111720A1 (fr) | 2018-08-31 | 2020-03-05 | The Charles Stark Draper Laboratory, Inc. | Procede et appareil de transfection a haut rendement et a haute efficacite de cellules |
| US11071860B2 (en) | 2019-02-06 | 2021-07-27 | Oncosec Medical Incorporated | Systems and methods for detecting fault conditions in electroporation therapy |
| JP7248721B2 (ja) * | 2021-02-17 | 2023-03-29 | ゼネラル・エレクトリック・カンパニイ | 成長因子レベルを調節可能な活性化血小板組成物 |
| JPWO2022224803A1 (fr) | 2021-04-22 | 2022-10-27 | ||
| WO2023223931A1 (fr) * | 2022-05-16 | 2023-11-23 | 富士フイルム株式会社 | Dispositif d'électroporation et procédé d'électroporation |
-
1996
- 1996-03-11 KR KR1019970706255A patent/KR19980702848A/ko not_active Application Discontinuation
- 1996-03-11 AU AU53050/96A patent/AU5305096A/en not_active Abandoned
- 1996-03-11 CN CN96193177A patent/CN1195997A/zh active Pending
- 1996-03-11 CA CA002214800A patent/CA2214800C/fr not_active Expired - Lifetime
- 1996-03-11 EP EP96909619A patent/EP0814855A4/fr not_active Withdrawn
- 1996-03-11 JP JP52774896A patent/JP3859709B2/ja not_active Expired - Lifetime
-
2006
- 2006-07-25 JP JP2006202504A patent/JP4171755B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP4171755B2 (ja) | 2008-10-29 |
| AU5305096A (en) | 1996-10-02 |
| KR19980702848A (ko) | 1998-08-05 |
| JP2007007430A (ja) | 2007-01-18 |
| CN1195997A (zh) | 1998-10-14 |
| CA2214800A1 (fr) | 1996-09-19 |
| EP0814855A4 (fr) | 2002-07-17 |
| EP0814855A1 (fr) | 1998-01-07 |
| JPH11502133A (ja) | 1999-02-23 |
| JP3859709B2 (ja) | 2006-12-20 |
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| Date | Code | Title | Description |
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| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20160311 |