BRPI0312441B8 - método para produzir um pré-adipócitos de cultura primária para terapia genética e composição de implante para terapia genética - Google Patents

método para produzir um pré-adipócitos de cultura primária para terapia genética e composição de implante para terapia genética

Info

Publication number
BRPI0312441B8
BRPI0312441B8 BRPI0312441A BR0312441A BRPI0312441B8 BR PI0312441 B8 BRPI0312441 B8 BR PI0312441B8 BR PI0312441 A BRPI0312441 A BR PI0312441A BR 0312441 A BR0312441 A BR 0312441A BR PI0312441 B8 BRPI0312441 B8 BR PI0312441B8
Authority
BR
Brazil
Prior art keywords
gene therapy
cells
present
adipocytes
preadipocyte
Prior art date
Application number
BRPI0312441A
Other languages
English (en)
Other versions
BRPI0312441B1 (pt
BR0312441A (pt
Inventor
Ito Masashi
Saito Yasushi
Original Assignee
Eisai Co Ltd
Eisai R&D Man Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eisai Co Ltd, Eisai R&D Man Co Ltd filed Critical Eisai Co Ltd
Publication of BR0312441A publication Critical patent/BR0312441A/pt
Publication of BRPI0312441B1 publication Critical patent/BRPI0312441B1/pt
Publication of BRPI0312441B8 publication Critical patent/BRPI0312441B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Diabetes (AREA)
  • Rheumatology (AREA)
  • Endocrinology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Emergency Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

"adipócitos de cultura primária para terapia genética". a presente invenção refere-se a adipócitos de cultura primária para terapia genética, onde os adipócitos mantêm de forma estável um gene estranho que codifica uma proteína que é secretada fora de células. esta invenção fornece células apropriadas para terapia genética, que podem repor células da medula óssea e células hepáticas usadas para terapia genética ex vivo convencional. a presente invenção estabeleceu métodos para transferir genes estranhos para dentro de adipócitos de cultura primária, que são apropriados para terapia genética ex vivo; pode ser coletados e implantados facilmente; e podem ser removidos após o implante. especificamente, a presente invenção estabeleceu métodos tais que usam vetores retrovirais. a presente invenção estabeleceu também adipócitos de cultura primária para terapia genética, onde os adipócitos mantêm de forma estável um gene estranho que codifica uma proteína que é secretada fora de células.
BRPI0312441A 2002-06-18 2003-06-18 método para produzir um pré-adipócitos de cultura primária para terapia genética e composição de implante para terapia genética BRPI0312441B8 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2002/177648 2002-06-18
JP2002177648 2002-06-18
JP2002/237974 2002-08-19
JP2002237974 2002-08-19
PCT/JP2003/007721 WO2003106663A1 (ja) 2002-06-18 2003-06-18 遺伝子治療用初代培養脂肪細胞

Publications (3)

Publication Number Publication Date
BR0312441A BR0312441A (pt) 2005-04-19
BRPI0312441B1 BRPI0312441B1 (pt) 2018-03-20
BRPI0312441B8 true BRPI0312441B8 (pt) 2021-05-25

Family

ID=29738440

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0312441A BRPI0312441B8 (pt) 2002-06-18 2003-06-18 método para produzir um pré-adipócitos de cultura primária para terapia genética e composição de implante para terapia genética

Country Status (20)

Country Link
US (2) US7820438B2 (pt)
EP (2) EP2213730B1 (pt)
JP (1) JPWO2003106663A1 (pt)
KR (1) KR101004086B1 (pt)
CN (1) CN1671836B (pt)
AT (1) ATE492630T1 (pt)
AU (1) AU2003242459B2 (pt)
BR (1) BRPI0312441B8 (pt)
CA (1) CA2488414C (pt)
DE (1) DE60335473D1 (pt)
DK (2) DK1541674T3 (pt)
ES (1) ES2559430T3 (pt)
HU (1) HUE025872T2 (pt)
MA (1) MA27324A1 (pt)
MX (1) MXPA04012859A (pt)
NZ (1) NZ537072A (pt)
PT (2) PT2213730E (pt)
SI (1) SI1541674T1 (pt)
WO (1) WO2003106663A1 (pt)
ZA (1) ZA200500464B (pt)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4879867B2 (ja) * 2002-06-18 2012-02-22 エーザイ・アール・アンド・ディー・マネジメント株式会社 遺伝子治療用初代培養脂肪細胞
US8835177B2 (en) 2005-06-15 2014-09-16 Takara Bio Inc. Method for transfer of gene into fat cell or progenitor fat cell
JPWO2008108344A1 (ja) * 2007-03-02 2010-06-17 セルジェンテック株式会社 Ｌｃａｔ欠損症の遺伝子治療用細胞並びに遺伝子治療用細胞組成物
TW200927074A (en) * 2007-12-25 2009-07-01 Univ Nat Taiwan Colloidal frame used in tissue engineering
JP2009165403A (ja) * 2008-01-16 2009-07-30 Hokkaido Univ ハニカム状多孔質体を用いた脂肪細胞の長期培養方法
EP2350274B1 (en) 2008-10-21 2019-01-23 The General Hospital Corporation Cell transplantation
WO2011090091A1 (ja) * 2010-01-21 2011-07-28 セルジェンテック株式会社 遺伝子治療用脂肪細胞の調製のための外来遺伝子の導入に適した細胞集団かどうかを判定する方法
BR112013002859B1 (pt) 2010-08-06 2020-10-06 The General Hospital Corporation D/B/A Massachusetts General Hospital Método e aparelho para tratamento de células
US20140065659A1 (en) * 2011-03-16 2014-03-06 Kuraray Co., Ltd. Culture method, group of mature adipocytes, drug screening method
JP2023002772A (ja) * 2011-09-23 2023-01-10 レジニアス リミテッド 脂肪細胞および細胞分泌物を使用する治療
US20230210945A1 (en) 2020-05-29 2023-07-06 Numt Inc. Adipocytes Over-Expressing FFAR4 and Use Thereof
EP4213863A1 (en) * 2020-09-15 2023-07-26 The Brigham and Women's Hospital, Inc. In vivo adipose bioreactor and kits for the production and delivery of biologic agents
WO2023249904A1 (en) * 2022-06-20 2023-12-28 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for treating diabetes

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4956455A (en) 1984-03-05 1990-09-11 The Salk Institute For Biological Studies Bovine fibroblast growth factor
US4829000A (en) 1985-08-30 1989-05-09 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Reconstituted basement membrane complex with biological activity
IE66689B1 (en) 1985-09-12 1996-01-24 Scios Nova Inc Recombinant fibroblast growth factors
IL80944A (en) 1985-12-17 1994-10-07 Synergen Inc Boulder Colo U S A factor in the formation of blood vessels in a human placenta capable of inducing protease synthesis in the inner lining of capillary cells, DNA synthesis. And migration of materials
DK122687A (da) 1986-03-14 1987-09-15 Takeda Chemical Industries Ltd Polypeptid, dna og anvendelse deraf
JP2526965B2 (ja) 1987-02-24 1996-08-21 武田薬品工業株式会社 ムテイン，ｄｎａおよびその用途
WO1989004832A1 (en) 1987-11-24 1989-06-01 Amgen Inc. Analogs of fibroblast growth factor
EP0326907A1 (en) 1988-01-26 1989-08-09 Takeda Chemical Industries, Ltd. Polypeptide, DNA and its use
CA2015313A1 (en) 1989-04-26 1990-10-26 Masaharu Senoo Glycosylated fgf and production thereof
JP3135138B2 (ja) 1990-12-19 2001-02-13 科研製薬株式会社 骨疾患治療剤
EP1179350A3 (en) * 1993-08-12 2003-01-02 Cytotherapeutics, Inc. Encapsulated cell system for implantation into the human CNS
FR2731014B1 (fr) 1995-02-23 1997-03-28 Rhone Poulenc Rorer Sa Molecules d'adn, preparation et utilisation en therapie genique
FR2731710B1 (fr) 1995-03-14 1997-04-30 Rhone Poulenc Rorer Sa Virus recombinants exprimant la lecithine cholesterol acyltransferase et utilisations en therapie genique
US5869037A (en) 1996-06-26 1999-02-09 Cornell Research Foundation, Inc. Adenoviral-mediated gene transfer to adipocytes
GB2327224A (en) 1997-07-09 1999-01-20 Mayo Foundation Gene transfer to adipocytes
WO1999011786A1 (fr) 1997-09-01 1999-03-11 Otsuka Pharmaceutical Co., Ltd. GENE pancpin ET COMPOSITION DE THERAPIE GENIQUE
CA2308575A1 (en) 1997-11-03 1999-05-14 Tom Tsang Hyperthermic inducible expression vectors for gene therapy and methods of use thereof
US6797505B2 (en) 1998-05-27 2004-09-28 Cell Genesys, Inc. Recombinant AAV vectors for gene therapy of hemophilia A
WO2000031267A1 (en) * 1998-11-20 2000-06-02 The Autonomous University Of Barcelona Insulin production by engineered muscle cells
US6153432A (en) * 1999-01-29 2000-11-28 Zen-Bio, Inc Methods for the differentiation of human preadipocytes into adipocytes
US7015037B1 (en) * 1999-08-05 2006-03-21 Regents Of The University Of Minnesota Multiponent adult stem cells and methods for isolation
DK1226233T3 (da) 1999-08-05 2011-10-03 Abt Holding Co Multipotente voksne stamceller og fremgangsmåder til isolering heraf
US20020177551A1 (en) * 2000-05-31 2002-11-28 Terman David S. Compositions and methods for treatment of neoplastic disease
JP2004504024A (ja) * 2000-07-18 2004-02-12 ソシエテ　デ　プロデユイ　ネツスル　ソシエテ　アノニム 前脂肪細胞系
US20050008621A1 (en) * 2001-10-06 2005-01-13 James Kirkland Preadipocyte cell strains and uses therefore
EP1496978A4 (en) * 2002-04-03 2006-11-29 Artecel Sciences Inc IMPROVEMENTS IN DIFFERENTIATED ADULT DIFFERENTIATED STEM CELLS DERIVED FROM ADIPOSE TISSUE AND USES THEREOF
JPWO2008108344A1 (ja) 2007-03-02 2010-06-17 セルジェンテック株式会社 Ｌｃａｔ欠損症の遺伝子治療用細胞並びに遺伝子治療用細胞組成物

Also Published As

Publication number Publication date
KR101004086B1 (ko) 2010-12-27
US8071085B2 (en) 2011-12-06
EP1541674B9 (en) 2011-09-14
DK1541674T3 (da) 2011-03-14
CA2488414A1 (en) 2003-12-24
EP2213730B1 (en) 2015-12-02
BRPI0312441B1 (pt) 2018-03-20
SI1541674T1 (sl) 2011-04-29
CA2488414C (en) 2011-04-26
EP2213730A1 (en) 2010-08-04
AU2003242459B2 (en) 2007-05-17
PT2213730E (pt) 2016-02-26
EP1541674A1 (en) 2005-06-15
PT1541674E (pt) 2011-03-21
KR20070087062A (ko) 2007-08-27
CN1671836B (zh) 2012-02-08
JPWO2003106663A1 (ja) 2005-10-13
BR0312441A (pt) 2005-04-19
DK2213730T3 (en) 2016-01-25
US20060121008A1 (en) 2006-06-08
WO2003106663A1 (ja) 2003-12-24
HUE025872T2 (en) 2016-05-30
ES2559430T3 (es) 2016-02-12
MA27324A1 (fr) 2005-05-02
ATE492630T1 (de) 2011-01-15
MXPA04012859A (es) 2005-03-31
EP2213730A8 (en) 2010-11-10
US20090010901A1 (en) 2009-01-08
CN1671836A (zh) 2005-09-21
DE60335473D1 (de) 2011-02-03
NZ537072A (en) 2006-12-22
US7820438B2 (en) 2010-10-26
ZA200500464B (en) 2005-12-28
EP1541674B1 (en) 2010-12-22
EP1541674A4 (en) 2007-01-10
AU2003242459A1 (en) 2003-12-31

Similar Documents

Publication Publication Date Title
MA27324A1 (fr) Adipocytes de culture primaire utilises en therapie genique.
Onofrillo et al. Biofabrication of human articular cartilage: a path towards the development of a clinical treatment
US20060239980A1 (en) Cartilage-derived stem cells and applications thereof
Neupane et al. Isolation and characterization of canine adipose–derived mesenchymal stem cells
Nesti et al. Differentiation potential of multipotent progenitor cells derived from war-traumatized muscle tissue
Smith et al. Isolation and implantation of autologous equine mesenchymal stem cells from bone marrow into the superficial digital flexor tendon as a potential novel treatment
ES2291253T3 (es) Procedimiento para la produccion de implantes de cartilago por medio de condrocitos cultivados in vitro.
JP5764807B2 (ja) 軟骨欠損の修復に用いる外科用グラフト
PT1062321E (pt) Utilizacoes para celulas estaminais mesenquimais humanas nao autologas
CN109152864A (zh) 一种用于制备三维软骨类器官块的方法
HUP0401945A2 (hu) Zsírszövetből származó stromasejtek, és azok alkalmazása a szaruhártya hibáinak és szemen belüli fogyatékosságok javítására
ES2230157T3 (es) Aislamiento de celulas precursoras y su uso para la reparacion de tejidos.
US20130029292A1 (en) Method of implanting mesenchymal stem cells for natural tooth regeneration in surgically prepared extraction socket and compositions thereof
DK1274464T3 (da) Sammensætninger og fremgangsmåder til regulering af proteinekspression i tarm
Deans et al. Stem cells in musculoskeletal engineered tissue
MX2011000993A (es) Metodo para separar celulas madre activas de celulas madre humanas y celulas madre altamente activas separadas por el mismo.
WO2003011343A1 (en) Method of regenerating bone/chondral tissues by transferring transcriptional factor gene
Mikael et al. A potential translational approach for bone tissue engineering through endochondral ossification
WO2008153813A8 (en) Bone augmentation utilizing muscle-derived progenitor compositions, and treatments thereof
US20090098591A1 (en) Method of Correlating Marker Molecule Concentration To A Specific Cell Potency Level in Chondrocyte Culture
CN103255101A (zh) 一种成釉细胞的分离培养方法
JP2020525107A (ja) 移植可能な軟骨組織の製造方法
MXPA02002444A (es) Metodo para generar celulas y tejidos inmunocompatibles, utilizando tecnicas de transferencia nuclear.
NO20050248L (no) Primaere, dyrkede adipocytter for genterapi
Zong et al. Isolation, culture and biological characteristics of duck periosteum stem cells in vitro

Legal Events

Date Code Title Description
B25A Requested transfer of rights approved

Owner name: EISAI RANDD MANAGEMENT CO., LTD. (JP)

Free format text: TRANSFERIDO DE: EISAI CO., LTD.

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 20/03/2018, OBSERVADAS AS CONDICOES LEGAIS.

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 18/06/2003 OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 21A ANUIDADE.