BR112015013647A2 - variante isolada de uma enzima celobio-hidrolase (cbh) parental, polinucleotídeo isolado, vetor, célula hospedeira, composição detergente, aditivo alimentar, método para hidrolisar um substrato celulósico, sobrenadante de cultura celular, métodos de produção de um polipeptídeo cbh variante e sobrenadante de cultura celular - Google Patents

variante isolada de uma enzima celobio-hidrolase (cbh) parental, polinucleotídeo isolado, vetor, célula hospedeira, composição detergente, aditivo alimentar, método para hidrolisar um substrato celulósico, sobrenadante de cultura celular, métodos de produção de um polipeptídeo cbh variante e sobrenadante de cultura celular

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Publication number
BR112015013647A2
BR112015013647A2 BR112015013647A BR112015013647A BR112015013647A2 BR 112015013647 A2 BR112015013647 A2 BR 112015013647A2 BR 112015013647 A BR112015013647 A BR 112015013647A BR 112015013647 A BR112015013647 A BR 112015013647A BR 112015013647 A2 BR112015013647 A2 BR 112015013647A2
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BR
Brazil
Prior art keywords
cell culture
variant
cbh
supernatant
isolated
Prior art date
Application number
BR112015013647A
Other languages
English (en)
Inventor
R Kelemen Bradley
Nikolaev Igor
Van Lieshout Johannes
Foukaraki Maria
Sandgren Mats
R Bott Richard
Hommes Ronaldus
Van Stigt Thans Sander
Kralj Slavko
Kaper Thijs
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of BR112015013647A2 publication Critical patent/BR112015013647A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

resumo patente de invenção: "variantes de celobio-hidrolases". a presente invenção refere-se a vários homólogos e variantes de cel7a de hypocrea jecorina (anteriormente celobio-hidrolase 1 ou cbh1 de trichoderma reesei), ácidos nucleicos que codificam os mesmos e métodos para produzir os mesmos. as celulases homólogas e variantes têm a sequência de aminoácidos de uma glicosil hidrolase da família 7a em que um ou mais resíduos de aminoácidos são substituídos e/ou deletados.
BR112015013647A 2012-12-12 2013-12-10 variante isolada de uma enzima celobio-hidrolase (cbh) parental, polinucleotídeo isolado, vetor, célula hospedeira, composição detergente, aditivo alimentar, método para hidrolisar um substrato celulósico, sobrenadante de cultura celular, métodos de produção de um polipeptídeo cbh variante e sobrenadante de cultura celular BR112015013647A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261736315P 2012-12-12 2012-12-12
PCT/US2013/074005 WO2014093275A1 (en) 2012-12-12 2013-12-10 Variants of cellobiohydrolases

Publications (1)

Publication Number Publication Date
BR112015013647A2 true BR112015013647A2 (pt) 2017-11-14

Family

ID=49887301

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015013647A BR112015013647A2 (pt) 2012-12-12 2013-12-10 variante isolada de uma enzima celobio-hidrolase (cbh) parental, polinucleotídeo isolado, vetor, célula hospedeira, composição detergente, aditivo alimentar, método para hidrolisar um substrato celulósico, sobrenadante de cultura celular, métodos de produção de um polipeptídeo cbh variante e sobrenadante de cultura celular

Country Status (8)

Country Link
US (1) US9938551B2 (pt)
EP (2) EP2931887A1 (pt)
KR (1) KR20150093190A (pt)
CN (1) CN104870644A (pt)
BR (1) BR112015013647A2 (pt)
CA (1) CA2892786A1 (pt)
MX (1) MX2015007234A (pt)
WO (1) WO2014093275A1 (pt)

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EP3433358B1 (en) * 2016-03-24 2022-07-06 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
CN105969753B (zh) * 2016-07-15 2019-08-20 湖北工业大学 一种提高黑曲霉纤维素外切酶在高盐溶液中热稳定性的方法
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WO2019074828A1 (en) 2017-10-09 2019-04-18 Danisco Us Inc CELLOBIOSE DEHYDROGENASE VARIANTS AND METHODS OF USE
CN109797160A (zh) * 2018-09-26 2019-05-24 天津科技大学 一种毕赤酵母中异源表达外切葡聚糖酶Cel6A,Cel7A的构建方法
CN109456954B (zh) * 2018-11-21 2021-08-20 中山大学 热稳定性提高的β-葡萄糖苷酶突变体及其应用
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WO2023102315A1 (en) 2021-12-01 2023-06-08 Danisco Us Inc. Compositions and methods for enhanced protein production in fungal cells

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Also Published As

Publication number Publication date
MX2015007234A (es) 2015-10-29
CA2892786A1 (en) 2014-06-19
CN104870644A (zh) 2015-08-26
EP3290515A1 (en) 2018-03-07
US20160201104A1 (en) 2016-07-14
US9938551B2 (en) 2018-04-10
WO2014093275A1 (en) 2014-06-19
EP2931887A1 (en) 2015-10-21
KR20150093190A (ko) 2015-08-17

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