BR112014031080A2 - métodos e composições de geração de alelos knock-out condicionais. - Google Patents
métodos e composições de geração de alelos knock-out condicionais.Info
- Publication number
- BR112014031080A2 BR112014031080A2 BR112014031080A BR112014031080A BR112014031080A2 BR 112014031080 A2 BR112014031080 A2 BR 112014031080A2 BR 112014031080 A BR112014031080 A BR 112014031080A BR 112014031080 A BR112014031080 A BR 112014031080A BR 112014031080 A2 BR112014031080 A2 BR 112014031080A2
- Authority
- BR
- Brazil
- Prior art keywords
- compositions
- methods
- conditional knock
- generating conditional
- out alleles
- Prior art date
Links
- 108700028369 Alleles Proteins 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0276—Knock-out vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Veterinary Medicine (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Animal Behavior & Ethology (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261658670P | 2012-06-12 | 2012-06-12 | |
PCT/US2013/045382 WO2013188522A2 (en) | 2012-06-12 | 2013-06-12 | Methods and compositions for generating conditional knock-out alleles |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112014031080A2 true BR112014031080A2 (pt) | 2018-05-08 |
Family
ID=49758880
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112014031080A BR112014031080A2 (pt) | 2012-06-12 | 2013-06-12 | métodos e composições de geração de alelos knock-out condicionais. |
Country Status (11)
Country | Link |
---|---|
US (1) | US20150128300A1 (pt) |
EP (1) | EP2858486A4 (pt) |
JP (1) | JP6279562B2 (pt) |
KR (1) | KR20150023670A (pt) |
CN (1) | CN104540382A (pt) |
BR (1) | BR112014031080A2 (pt) |
CA (1) | CA2876076A1 (pt) |
HK (1) | HK1209276A1 (pt) |
MX (1) | MX2014015204A (pt) |
RU (1) | RU2014153918A (pt) |
WO (1) | WO2013188522A2 (pt) |
Families Citing this family (77)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
US9834786B2 (en) | 2012-04-25 | 2017-12-05 | Regeneron Pharmaceuticals, Inc. | Nuclease-mediated targeting with large targeting vectors |
MX362866B (es) | 2012-05-25 | 2019-02-20 | Univ California | Metodos y composiciones para la modificacion de adn objetivo dirigida por arn y para la modulacion de la transcripcion dirigida por arn. |
EP3372679A1 (en) * | 2012-10-23 | 2018-09-12 | Toolgen Incorporated | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof |
PT3138910T (pt) | 2012-12-06 | 2017-10-18 | Sigma Aldrich Co Llc | Modificação e regulação de genoma baseado em crispr |
WO2014131833A1 (en) * | 2013-02-27 | 2014-09-04 | Helmholtz Zentrum München Deutsches Forschungszentrum Für Gesundheit Und Umwelt (Gmbh) | Gene editing in the oocyte by cas9 nucleases |
JP2016519652A (ja) | 2013-03-14 | 2016-07-07 | カリブー・バイオサイエンシーズ・インコーポレイテッド | 核酸ターゲティング核酸の組成物および方法 |
SG11201508028QA (en) | 2013-04-16 | 2015-10-29 | Regeneron Pharma | Targeted modification of rat genome |
US9593356B2 (en) * | 2013-06-11 | 2017-03-14 | Takara Bio Usa, Inc. | Protein enriched microvesicles and methods of making and using the same |
US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
TW201542816A (zh) | 2013-09-18 | 2015-11-16 | Kymab Ltd | 方法、細胞與生物體 |
US10584358B2 (en) | 2013-10-30 | 2020-03-10 | North Carolina State University | Compositions and methods related to a type-II CRISPR-Cas system in Lactobacillus buchneri |
KR102380245B1 (ko) | 2013-11-07 | 2022-03-30 | 에디타스 메디신, 인코포레이티드 | 지배적인 gRNA를 이용하는 CRISPR-관련 방법 및 조성물 |
CN105980568B (zh) | 2013-12-11 | 2019-12-03 | 瑞泽恩制药公司 | 用于靶向修饰基因组的方法和组合物 |
US20150166982A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting pi3k point mutations |
CN103725712B (zh) * | 2014-01-17 | 2016-03-30 | 上海金卫生物技术有限公司 | 一种无物种限制的条件性基因敲除用中间载体及其制备方法和用途 |
US10787654B2 (en) | 2014-01-24 | 2020-09-29 | North Carolina State University | Methods and compositions for sequence guiding Cas9 targeting |
US11439712B2 (en) | 2014-04-08 | 2022-09-13 | North Carolina State University | Methods and compositions for RNA-directed repression of transcription using CRISPR-associated genes |
US20170029805A1 (en) * | 2014-04-14 | 2017-02-02 | Maxcyte, Inc. | Methods and compositions for modifying genomic dna |
WO2015191911A2 (en) * | 2014-06-12 | 2015-12-17 | Clontech Laboratories, Inc. | Protein enriched microvesicles and methods of making and using the same |
US11254933B2 (en) * | 2014-07-14 | 2022-02-22 | The Regents Of The University Of California | CRISPR/Cas transcriptional modulation |
US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
WO2016025759A1 (en) | 2014-08-14 | 2016-02-18 | Shen Yuelei | Dna knock-in system |
CN107075546B (zh) * | 2014-08-19 | 2021-08-31 | 哈佛学院董事及会员团体 | 用于对核酸探测并作图的rna-引导的*** |
EP3186375A4 (en) | 2014-08-28 | 2019-03-13 | North Carolina State University | NEW CAS9 PROTEINS AND GUIDING ELEMENTS FOR DNA TARGETING AND THE GENOME EDITION |
CN104293833B (zh) * | 2014-10-09 | 2017-10-10 | 西北农林科技大学 | 一种基于TALEN介导的Sp110巨噬细胞特异打靶载体及重组细胞 |
CN104404036B (zh) * | 2014-11-03 | 2017-12-01 | 赛业(苏州)生物科技有限公司 | 基于CRISPR/Cas9技术的条件性基因敲除方法 |
WO2016073990A2 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
JP6190995B2 (ja) | 2014-11-17 | 2017-09-06 | 国立大学法人 東京医科歯科大学 | 簡便で高効率の遺伝子改変非ヒト哺乳動物の作製方法 |
CN113444747A (zh) | 2014-11-21 | 2021-09-28 | 瑞泽恩制药公司 | 使用成对向导rna进行靶向遗传修饰的方法和组合物 |
ES2926467T3 (es) | 2015-04-13 | 2022-10-26 | Maxcyte Inc | Métodos y composiciones para la modificación del ADN genómico |
KR20220139447A (ko) | 2015-05-29 | 2022-10-14 | 노쓰 캐롤라이나 스테이트 유니버시티 | 크리스퍼 핵산을 사용하여 박테리아, 고세균, 조류 및 효모를 스크리닝하는 방법 |
CN115537421A (zh) | 2015-06-15 | 2022-12-30 | 北卡罗来纳州立大学 | 有效递送核酸和基于rna的抗微生物剂的方法和组合物 |
US11279928B2 (en) | 2015-06-29 | 2022-03-22 | Massachusetts Institute Of Technology | Compositions comprising nucleic acids and methods of using the same |
US20180320186A1 (en) * | 2015-08-20 | 2018-11-08 | Inter-University Research Institute Corporation Research Organization Of Information And Systems | Method for site-specific insertion of foreign dna into a genome in an animal cell and a cell obtained using same |
CA2999500A1 (en) | 2015-09-24 | 2017-03-30 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
WO2017058751A1 (en) | 2015-09-28 | 2017-04-06 | North Carolina State University | Methods and compositions for sequence specific antimicrobials |
EP4269577A3 (en) | 2015-10-23 | 2024-01-17 | President and Fellows of Harvard College | Nucleobase editors and uses thereof |
CA3006781A1 (en) * | 2015-12-07 | 2017-06-15 | Arc Bio, Llc | Methods and compositions for the making and using of guide nucleic acids |
WO2017112620A1 (en) | 2015-12-22 | 2017-06-29 | North Carolina State University | Methods and compositions for delivery of crispr based antimicrobials |
CN107022572A (zh) * | 2016-02-01 | 2017-08-08 | 河北伊维沃生物科技有限公司 | Ldl受体基因敲除的基因工程仓鼠 |
WO2017136794A1 (en) | 2016-02-03 | 2017-08-10 | Massachusetts Institute Of Technology | Structure-guided chemical modification of guide rna and its applications |
CN114908093A (zh) * | 2016-02-26 | 2022-08-16 | 朗泽科技新西兰有限公司 | 用于c1固定菌的crispr/cas*** |
WO2017165826A1 (en) | 2016-03-25 | 2017-09-28 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
EP4047092A1 (en) | 2016-04-13 | 2022-08-24 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
CN109688810B (zh) | 2016-06-27 | 2022-06-03 | 贝勒医学院 | 具有缺陷性p450氧化还原酶的人肝嵌合非人动物及其使用方法 |
SG11201900907YA (en) | 2016-08-03 | 2019-02-27 | Harvard College | Adenosine nucleobase editors and uses thereof |
WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
CN107779462B (zh) * | 2016-08-29 | 2021-06-04 | 中国科学院分子细胞科学卓越创新中心 | 双同源重组谱系示踪技术 |
CN110214180A (zh) | 2016-10-14 | 2019-09-06 | 哈佛大学的校长及成员们 | 核碱基编辑器的aav递送 |
GB201617559D0 (en) | 2016-10-17 | 2016-11-30 | University Court Of The University Of Edinburgh The | Swine comprising modified cd163 and associated methods |
CN109906030B (zh) | 2016-11-04 | 2022-03-18 | 安健基因公司 | 用于产生仅重链抗体的经基因修饰的非人动物和方法 |
EP3546575A4 (en) * | 2016-11-28 | 2019-12-11 | Osaka University | GENOMEDITIERUNGSVERFAHREN |
US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
TW201839136A (zh) | 2017-02-06 | 2018-11-01 | 瑞士商諾華公司 | 治療血色素異常症之組合物及方法 |
EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
EP3592777A1 (en) | 2017-03-10 | 2020-01-15 | President and Fellows of Harvard College | Cytosine to guanine base editor |
CN110914426A (zh) | 2017-03-23 | 2020-03-24 | 哈佛大学的校长及成员们 | 包含核酸可编程dna结合蛋白的核碱基编辑器 |
WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
EP3652312A1 (en) | 2017-07-14 | 2020-05-20 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
WO2019023680A1 (en) | 2017-07-28 | 2019-01-31 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE) |
US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
JP2021500036A (ja) | 2017-10-16 | 2021-01-07 | ザ ブロード インスティテュート, インコーポレーテッドThe Broad Institute, Inc. | アデノシン塩基編集因子の使用 |
CN108998483B (zh) * | 2018-08-17 | 2021-04-30 | 武汉核圣生物技术有限公司 | 利用单亚基RNA聚合酶进行体外转录合成sgRNA的方法 |
US10711267B2 (en) | 2018-10-01 | 2020-07-14 | North Carolina State University | Recombinant type I CRISPR-Cas system |
BR112021018607A2 (pt) * | 2019-03-19 | 2021-11-23 | Massachusetts Inst Technology | Métodos e composições para editar sequências de nucleotídeos |
CN110512005B (zh) * | 2019-06-05 | 2021-05-14 | 浙江大学 | 泛素特异性蛋白酶8在调控湖羊肉质及育种中的应用 |
CN116096873A (zh) | 2020-05-08 | 2023-05-09 | 布罗德研究所股份有限公司 | 同时编辑靶标双链核苷酸序列的两条链的方法和组合物 |
CN113678789A (zh) * | 2021-08-26 | 2021-11-23 | 嘉兴学院 | Mir-379/410基因簇敲除的小鼠模型及其构建方法 |
GB202118058D0 (en) | 2021-12-14 | 2022-01-26 | Univ Warwick | Methods to increase yields in crops |
GB2621813A (en) | 2022-06-30 | 2024-02-28 | Univ Newcastle | Preventing disease recurrence in Mitochondrial replacement therapy |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US713608A (en) | 1901-07-06 | 1902-11-18 | William J Condlon | Course-indicator for ships. |
US5436150A (en) | 1992-04-03 | 1995-07-25 | The Johns Hopkins University | Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease |
WO1994018313A1 (en) | 1993-02-12 | 1994-08-18 | The Johns-Hopkins University | Functional domains in flavobacterium okeanokoites (foki) restriction endonuclease |
US7135608B1 (en) | 1997-08-28 | 2006-11-14 | The Salk Institute For Biological Studies | Site-specific recombination in eukaryotes and constructs useful therefor |
US6534261B1 (en) | 1999-01-12 | 2003-03-18 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
BR0209563A (pt) * | 2001-05-11 | 2004-12-07 | Genome Theraupeutics Corp | ácido nucleico, polipeptìdeo, vetor, célula, anticorpo, métodos de diagnosticar um fenótipo e um paciente como expressando um ácido nucleico e de identificar agentes que modulam a atividade de um ácido nucleico e de uma proteìna e lrp5, lrp6, ou hbm e compostos que modulam a interação de dkk com o caminho de sinalização wnt, composição para modular a massa óssea e/ou os nìveis de lipìdeo em um paciente, animal transgênico, embrião e modelo de animal, e, agente |
US20070218071A1 (en) * | 2003-09-15 | 2007-09-20 | Morris David W | Novel therapeutic targets in cancer |
EP1913149A4 (en) * | 2005-07-26 | 2009-08-05 | Sangamo Biosciences Inc | TARGETED INTEGRATION AND EXPRESSION OF EXOGENOUS NUCLEIC ACID SEQUENCES |
AU2007339753A1 (en) * | 2006-12-29 | 2008-07-10 | Osprey Pharmaceuticals Usa, Inc. | Methods of selecting and producing modified toxins, conjugates containing modified toxins and uses thereof |
DK2602323T3 (en) * | 2007-06-01 | 2018-04-16 | Open Monoclonal Tech Inc | Compositions and Methods for Inhibiting Endogenous Immunoglobin Genes and Producing Transgenic Human Idiotypic Antibodies |
US8936936B2 (en) * | 2007-10-25 | 2015-01-20 | Sangamo Biosciences, Inc. | Methods and compositions for targeted integration |
US20110023143A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Genomic editing of neurodevelopmental genes in animals |
CA2767377A1 (en) * | 2009-07-24 | 2011-01-27 | Sigma-Aldrich Co. Llc | Method for genome editing |
US20120192298A1 (en) * | 2009-07-24 | 2012-07-26 | Sigma Aldrich Co. Llc | Method for genome editing |
AU2010327998B2 (en) | 2009-12-10 | 2015-11-12 | Iowa State University Research Foundation, Inc. | TAL effector-mediated DNA modification |
US20120204282A1 (en) * | 2011-02-04 | 2012-08-09 | Sangamo Biosciences, Inc. | Methods and compositions for treating occular disorders |
MX362866B (es) * | 2012-05-25 | 2019-02-20 | Univ California | Metodos y composiciones para la modificacion de adn objetivo dirigida por arn y para la modulacion de la transcripcion dirigida por arn. |
-
2013
- 2013-06-12 US US14/407,869 patent/US20150128300A1/en not_active Abandoned
- 2013-06-12 RU RU2014153918A patent/RU2014153918A/ru not_active Application Discontinuation
- 2013-06-12 JP JP2015517390A patent/JP6279562B2/ja not_active Expired - Fee Related
- 2013-06-12 MX MX2014015204A patent/MX2014015204A/es unknown
- 2013-06-12 EP EP13804001.9A patent/EP2858486A4/en not_active Withdrawn
- 2013-06-12 CN CN201380042657.8A patent/CN104540382A/zh active Pending
- 2013-06-12 WO PCT/US2013/045382 patent/WO2013188522A2/en active Application Filing
- 2013-06-12 CA CA 2876076 patent/CA2876076A1/en not_active Abandoned
- 2013-06-12 BR BR112014031080A patent/BR112014031080A2/pt not_active IP Right Cessation
- 2013-06-12 KR KR1020157000353A patent/KR20150023670A/ko not_active Application Discontinuation
-
2015
- 2015-10-20 HK HK15110302.5A patent/HK1209276A1/xx unknown
Also Published As
Publication number | Publication date |
---|---|
WO2013188522A2 (en) | 2013-12-19 |
JP6279562B2 (ja) | 2018-02-14 |
EP2858486A2 (en) | 2015-04-15 |
KR20150023670A (ko) | 2015-03-05 |
EP2858486A4 (en) | 2016-04-13 |
HK1209276A1 (en) | 2016-04-01 |
JP2015519082A (ja) | 2015-07-09 |
MX2014015204A (es) | 2015-08-07 |
CA2876076A1 (en) | 2013-12-19 |
RU2014153918A (ru) | 2016-07-27 |
WO2013188522A3 (en) | 2014-04-10 |
US20150128300A1 (en) | 2015-05-07 |
CN104540382A (zh) | 2015-04-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
BR112014031080A2 (pt) | métodos e composições de geração de alelos knock-out condicionais. | |
IL269461B (en) | Cytidine-5-carboxamide modified nucleotide preparations and related methods | |
BR112015003227A2 (pt) | processos de clicoconjugação e composições | |
EP2971280A4 (en) | BIOSENSOR MICROARRAY COMPOSITIONS AND METHOD | |
SG11201602596UA (en) | Stabilized efinaconazole compositions | |
EP2832053A4 (en) | L3 GATEWAY FOR VXLAN | |
BR112014027477A2 (pt) | métodos de processamento de materiais lingnocelulósicos. | |
DK2895100T3 (da) | Digital bideskinne | |
GB201310108D0 (en) | Fragrancing composition | |
DK2875559T3 (da) | Kabeltætning | |
GB201209168D0 (en) | Crowd sourcing | |
BR112014032872A2 (pt) | processamento de biomassa. | |
SG11201605193YA (en) | Curable organosiloxane oligomer compositions | |
BR112013014204A2 (pt) | composições de sucops espumantes | |
BR112015009583A2 (pt) | instalação de distribuição de gás. | |
IL244318B (en) | Repairable organopolysiloxane compositions | |
SG11201502703XA (en) | Property prediction for asphalts from blended sources | |
BR112014027458A2 (pt) | composições de limpeza. | |
FR2986872B1 (fr) | . | |
ZA201508677B (en) | Stabilized mesembrine compositions | |
DK2831477T3 (da) | Støtteanordning til aflang genstand | |
SG11201604904XA (en) | Detergent and rinse-aid compositions and methods | |
BR112014026896A2 (pt) | mensagens de direitos digitais compactadas. | |
BR112013030369A2 (pt) | composições de cb-183,315 e métodos relacionados | |
BR112014027645A2 (pt) | 5-halogenopirazol bifenilcarboxamidas. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 6A ANUIDADE. |
|
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2518 DE 09-04-2019 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |
|
B350 | Update of information on the portal [chapter 15.35 patent gazette] |