CN103725712B - 一种无物种限制的条件性基因敲除用中间载体及其制备方法和用途 - Google Patents
一种无物种限制的条件性基因敲除用中间载体及其制备方法和用途 Download PDFInfo
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Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105087617A (zh) * | 2014-05-07 | 2015-11-25 | 中国农业科学院油料作物研究所 | 一种36bplox位点序列的重组载体及其构建方法与应用 |
CN103981215B (zh) * | 2014-05-23 | 2016-06-29 | 安徽省农业科学院水稻研究所 | 一种用于基因工程的骨干质粒载体及应用 |
CN104404036B (zh) * | 2014-11-03 | 2017-12-01 | 赛业(苏州)生物科技有限公司 | 基于CRISPR/Cas9技术的条件性基因敲除方法 |
CN105255898A (zh) * | 2015-11-27 | 2016-01-20 | 江西农业大学 | 一种沉默水稻过氧化氢酶基因OsCAT3的sgRNA |
CN105255885A (zh) * | 2015-11-27 | 2016-01-20 | 江西农业大学 | 一种沉默水稻过氧化氢酶基因OSCAT2的sgRNA |
CN105296487A (zh) * | 2015-11-27 | 2016-02-03 | 江西农业大学 | 一种沉默水稻过氧化氢酶基因OSCAT1的sgRNA |
CN105647885B (zh) * | 2016-01-20 | 2017-08-18 | 广州元曦生物科技有限公司 | Cas9融合蛋白及其编码序列 |
CN115044619B (zh) * | 2022-05-25 | 2023-09-15 | 郑州大学第一附属医院 | 一种全身诱导性Ppp3ca基因敲除鼠模型的构建方法 |
Citations (2)
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CN102226201A (zh) * | 2011-05-09 | 2011-10-26 | 新疆农垦科学院 | 一种表达载体及其构建和应用 |
WO2013188522A2 (en) * | 2012-06-12 | 2013-12-19 | Genentech, Inc. | Methods and compositions for generating conditional knock-out alleles |
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CN102226201A (zh) * | 2011-05-09 | 2011-10-26 | 新疆农垦科学院 | 一种表达载体及其构建和应用 |
WO2013188522A2 (en) * | 2012-06-12 | 2013-12-19 | Genentech, Inc. | Methods and compositions for generating conditional knock-out alleles |
Non-Patent Citations (5)
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Controlled gene expression in primary Lgr5 organoid cultures;Bon-Kyoung Koo1 等;《nature methods》;20120131;第9卷(第1期);81-84 * |
CRISPR/Cas9新型基因打靶***的研究进展;李辉 等;《江苏农业学报》;20130831;第29卷(第4期);907-911 * |
CRISPR/Cas***:RNA靶向的基因组定向编辑新技术;李君 等;《遗传》;20131130;第35卷(第11期);1265-1273 * |
Generating rats with conditional alleles using CRISPR/Cas9;Yuanwu Ma 等;《Cell Research》;20131231;第24卷;122-125 * |
One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering;Hui Yang 等;《CELL》;20130930;1370-1379 * |
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