ATE420170T1 - Matrizengerichtete split-and-mix-synthese von bibliotheken kleiner moleküle - Google Patents
Matrizengerichtete split-and-mix-synthese von bibliotheken kleiner moleküleInfo
- Publication number
- ATE420170T1 ATE420170T1 AT05806601T AT05806601T ATE420170T1 AT E420170 T1 ATE420170 T1 AT E420170T1 AT 05806601 T AT05806601 T AT 05806601T AT 05806601 T AT05806601 T AT 05806601T AT E420170 T1 ATE420170 T1 AT E420170T1
- Authority
- AT
- Austria
- Prior art keywords
- molecule
- oligonucleotide
- mattrice
- small molecule
- reaction wells
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA200401809 | 2004-11-22 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE420170T1 true ATE420170T1 (de) | 2009-01-15 |
Family
ID=36407493
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT05806601T ATE420170T1 (de) | 2004-11-22 | 2005-11-22 | Matrizengerichtete split-and-mix-synthese von bibliotheken kleiner moleküle |
Country Status (6)
Country | Link |
---|---|
US (8) | US8168381B2 (de) |
EP (1) | EP1828381B1 (de) |
AT (1) | ATE420170T1 (de) |
DE (1) | DE602005012319D1 (de) |
DK (1) | DK1828381T3 (de) |
WO (1) | WO2006053571A2 (de) |
Families Citing this family (73)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002102820A1 (en) | 2001-06-20 | 2002-12-27 | Nuevolution A/S | Nucleoside derivatives for library preparation |
DK1487978T3 (da) | 2002-03-15 | 2009-03-23 | Nuevolution As | Forbedret fremgangsmåde til syntese af templatemolekyler |
EP1539980B1 (de) | 2002-08-01 | 2016-02-17 | Nuevolution A/S | MEHRSTUFENSYNTHESE VON MIT MATRIZEN ASSOZIIERTE MOLEKüLEN |
EP1558744B1 (de) | 2002-10-30 | 2011-06-15 | Nuevolution A/S | Enzymatisches codieren |
WO2004056994A2 (en) | 2002-12-19 | 2004-07-08 | Nuevolution A/S | Quasirandom structure and function guided synthesis methods |
WO2004074429A2 (en) | 2003-02-21 | 2004-09-02 | Nuevolution A/S | Method for producing second-generation library |
WO2004083427A2 (en) | 2003-03-20 | 2004-09-30 | Nuevolution A/S | Ligational encoding of small molecules |
US11118215B2 (en) | 2003-09-18 | 2021-09-14 | Nuevolution A/S | Method for obtaining structural information concerning an encoded molecule and method for selecting compounds |
US7972994B2 (en) | 2003-12-17 | 2011-07-05 | Glaxosmithkline Llc | Methods for synthesis of encoded libraries |
EP2366705A1 (de) | 2003-12-17 | 2011-09-21 | Praecis Pharmaceuticals Incorporated | Verfahren zur Synthese von codierten Bibliotheken |
EP1730277B1 (de) | 2004-03-22 | 2009-10-28 | Nuevolution A/S | Ligationscodierung unter verwendung von oligonukleotidbausteinen |
ATE420170T1 (de) * | 2004-11-22 | 2009-01-15 | Peter Birk Rasmussen | Matrizengerichtete split-and-mix-synthese von bibliotheken kleiner moleküle |
PT1910538E (pt) * | 2005-06-09 | 2011-07-28 | Praecis Pharm Inc | Métodos para a síntese de bibliotecas codificadas |
EP2368868A1 (de) | 2005-10-28 | 2011-09-28 | Praecis Pharmaceuticals Inc. | Verfahren zur Identifikation von Verbindungen von Interesse mithilfe kodierter Bibliotheken |
LT3018206T (lt) | 2005-12-01 | 2021-12-10 | Nuevolution A/S | Fermentiniai kodavimo būdai, skirti didelių bibliotekų efektyviai sintezei |
GB0712245D0 (en) * | 2007-06-25 | 2007-08-01 | Cythera Technologies Ltd | Combinatorial libraries and related methods |
EP3653763A1 (de) | 2009-02-13 | 2020-05-20 | X-Chem, Inc. | Verfahren zur erstellung und durchsuchung von dna-codierten bibliotheken |
US8716191B2 (en) | 2009-06-22 | 2014-05-06 | Universite De Strasbourg | Method of preparing an adduct |
US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
SI2558577T1 (sl) | 2010-04-16 | 2019-05-31 | Nuevolution A/S | Bifunkcionalni kompleksi in metode za pripravo in uporabo takšnih kompleksov |
CN103119165B (zh) | 2010-09-27 | 2017-04-12 | 威泊根私人有限公司 | 制备富集文库的方法 |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
US9695530B2 (en) * | 2011-08-11 | 2017-07-04 | Deborah Jean Hall | Apparatus to facilitate the commencement and execution of off-loom bead weaving stitches and method(s) of using same |
LT3594340T (lt) * | 2011-08-26 | 2021-10-25 | Gen9, Inc. | Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai |
KR102124613B1 (ko) | 2011-09-07 | 2020-06-19 | 엑스-켐, 인크. | Dna-코딩된 라이브러리에 태그부착하는 방법 |
DK2872680T3 (en) | 2012-07-13 | 2018-07-09 | X Chem Inc | DNA-encoded libraries with coding oligonucleotide compounds that cannot be read with polymerases |
US10603385B2 (en) | 2013-06-04 | 2020-03-31 | Tribiotica Llc | Methods and compositions for templated assembly of nucleic acid specific heterocompounds |
US9879313B2 (en) | 2013-06-25 | 2018-01-30 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
US9765375B2 (en) | 2013-06-28 | 2017-09-19 | General Electric Company | Methods for developing binding-elements and uses thereof |
EP3146094B1 (de) * | 2014-05-23 | 2019-08-28 | Centrillion Technology Holdings Corporation | Oligonukleotidsondeninversionsverfahren für in-situ-synthetisierte sondenarrays |
EP3218479A1 (de) | 2014-11-11 | 2017-09-20 | Nanocore ApS | Verfahren zur identifizierung von molekülen mit gewünschten eigenschaften |
NL2013857B1 (en) * | 2014-11-21 | 2016-10-11 | Piculet Biosciences Tech B V | Self-assembled bivalent ligand complex (SABLC) libraries and methods for screening such libraries. |
CA2968552A1 (en) | 2014-12-02 | 2016-06-09 | Tribiotica Llc | Methods and kits for theranostic applications |
EP4119677B1 (de) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Räumlich getrennte multiplex-nukleinsäureanalyse von biologischen proben |
US10695735B2 (en) | 2015-08-18 | 2020-06-30 | Centrillion Technology Holdings Corporation | Probe inversion process for in situ synthesized probe arrays |
US11124791B2 (en) | 2015-09-14 | 2021-09-21 | Arizona Board Of Regents On Behalf Of Arizona State University | Generating recombinant affinity reagents with arrayed targets |
CN115820806A (zh) | 2016-06-16 | 2023-03-21 | 哈斯达克科学公司 | 寡核苷酸指导和记录的编码探针分子的组合合成 |
EP3541940A4 (de) * | 2016-11-21 | 2020-10-14 | Tribiotica Llc | Verfahren für gerichtete faltassemblierung oder dimerisierung von proteinen durch template-reaktionen |
US11186839B2 (en) | 2016-11-21 | 2021-11-30 | Tribiotica Llc | Methods for preventing titration of bimolecular templated assembly reactions by structurally-determined differential hybridizations |
CN110191953A (zh) * | 2016-11-21 | 2019-08-30 | 特里比奥迪卡有限责任公司 | 通过接近增强的反应性实现***蛋白模板组装的方法 |
WO2018166532A1 (en) | 2017-03-17 | 2018-09-20 | Hitgen Ltd. | Methods and compositions for synthesis of encoded libraries |
EP3619340A4 (de) | 2017-05-02 | 2021-01-20 | Haystack Sciences Corporation | Moleküle zur verifizierung von oligonukleotidgerichteter kombinatorischer synthese und verfahren zur herstellung und verwendung davon |
US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
US20220025447A1 (en) | 2018-12-10 | 2022-01-27 | 10X Genomics, Inc. | Generating spatial arrays with gradients |
US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
SG11202107913VA (en) | 2019-01-22 | 2021-08-30 | Vipergen Aps | A method for screening of an in vitro display library within a cell |
JP2022523362A (ja) * | 2019-02-21 | 2022-04-22 | ストラトス ゲノミクス インコーポレイテッド | 単一分子配列決定における使用のための拡張可能ポリマーの固体合成のための方法、組成物、およびデバイス |
WO2020243579A1 (en) | 2019-05-30 | 2020-12-03 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
CN112794874A (zh) * | 2019-11-13 | 2021-05-14 | 成都先导药物开发股份有限公司 | 一种On-DNA Petasis反应的合成方法 |
EP3891300B1 (de) | 2019-12-23 | 2023-03-29 | 10X Genomics, Inc. | Verfahren zur räumlichen analyse mittels rna-template-ligation |
US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
ES2965354T3 (es) | 2020-04-22 | 2024-04-12 | 10X Genomics Inc | Métodos para análisis espacial que usan eliminación de ARN elegido como diana |
WO2021237087A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
EP4153775A1 (de) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Simultane räumlich-zeitliche messung der genexpression und der zellaktivität |
WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
EP4162074B1 (de) | 2020-06-08 | 2024-04-24 | 10X Genomics, Inc. | Verfahren zur bestimmung eines chirurgischen randes und verfahren zur verwendung davon |
EP4165207A1 (de) | 2020-06-10 | 2023-04-19 | 10X Genomics, Inc. | Verfahren zur bestimmung einer position eines analyten in einer biologischen probe |
AU2021294334A1 (en) | 2020-06-25 | 2023-02-02 | 10X Genomics, Inc. | Spatial analysis of DNA methylation |
US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
EP4121555A1 (de) | 2020-12-21 | 2023-01-25 | 10X Genomics, Inc. | Verfahren, zusammensetzungen und systeme zur erfassung von sonden und/oder barcodes |
IL309435A (en) * | 2021-06-17 | 2024-02-01 | Insitro Inc | Methods of preparing bivalent molecules |
EP4196605A1 (de) | 2021-09-01 | 2023-06-21 | 10X Genomics, Inc. | Verfahren, zusammensetzungen und kits zur blockierung einer erfassungssonde auf einer räumlichen anordnung |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1993006121A1 (en) * | 1991-09-18 | 1993-04-01 | Affymax Technologies N.V. | Method of synthesizing diverse collections of oligomers |
US5573905A (en) | 1992-03-30 | 1996-11-12 | The Scripps Research Institute | Encoded combinatorial chemical libraries |
ES2271306T5 (es) | 2001-03-19 | 2013-10-16 | President And Fellows Of Harvard College | Evolución de una nueva función molecular |
NZ530098A (en) * | 2001-06-20 | 2006-02-24 | Nuevolution As | Templated molecules and methods for using such molecules |
DK1487978T3 (da) | 2002-03-15 | 2009-03-23 | Nuevolution As | Forbedret fremgangsmåde til syntese af templatemolekyler |
JP4657919B2 (ja) * | 2002-08-19 | 2011-03-23 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 進化する新しい分子機能 |
EP1558744B1 (de) * | 2002-10-30 | 2011-06-15 | Nuevolution A/S | Enzymatisches codieren |
WO2004083427A2 (en) * | 2003-03-20 | 2004-09-30 | Nuevolution A/S | Ligational encoding of small molecules |
EP2366705A1 (de) | 2003-12-17 | 2011-09-21 | Praecis Pharmaceuticals Incorporated | Verfahren zur Synthese von codierten Bibliotheken |
ATE420170T1 (de) * | 2004-11-22 | 2009-01-15 | Peter Birk Rasmussen | Matrizengerichtete split-and-mix-synthese von bibliotheken kleiner moleküle |
-
2005
- 2005-11-22 AT AT05806601T patent/ATE420170T1/de not_active IP Right Cessation
- 2005-11-22 DE DE200560012319 patent/DE602005012319D1/de active Active
- 2005-11-22 EP EP05806601A patent/EP1828381B1/de not_active Not-in-force
- 2005-11-22 US US11/719,846 patent/US8168381B2/en not_active Expired - Fee Related
- 2005-11-22 DK DK05806601T patent/DK1828381T3/da active
- 2005-11-22 WO PCT/DK2005/000747 patent/WO2006053571A2/en active Application Filing
-
2012
- 2012-04-09 US US13/442,236 patent/US20130237459A1/en not_active Abandoned
-
2013
- 2013-10-30 US US14/067,789 patent/US8951728B2/en not_active Expired - Fee Related
-
2014
- 2014-03-31 US US14/230,896 patent/US20140235509A1/en not_active Abandoned
-
2015
- 2015-01-06 US US14/590,848 patent/US20150126381A1/en not_active Abandoned
- 2015-04-01 US US14/676,381 patent/US20150203841A1/en not_active Abandoned
-
2018
- 2018-01-30 US US15/884,214 patent/US20180163199A1/en not_active Abandoned
- 2018-05-16 US US15/981,854 patent/US20180251756A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US20150126381A1 (en) | 2015-05-07 |
US20140066336A1 (en) | 2014-03-06 |
US20090209430A1 (en) | 2009-08-20 |
US20180251756A1 (en) | 2018-09-06 |
US20150203841A1 (en) | 2015-07-23 |
US20180163199A1 (en) | 2018-06-14 |
DK1828381T3 (da) | 2009-02-23 |
US8951728B2 (en) | 2015-02-10 |
US20140235509A1 (en) | 2014-08-21 |
US8168381B2 (en) | 2012-05-01 |
EP1828381B1 (de) | 2009-01-07 |
DE602005012319D1 (de) | 2009-02-26 |
WO2006053571A3 (en) | 2006-08-17 |
EP1828381A2 (de) | 2007-09-05 |
US20130237459A1 (en) | 2013-09-12 |
WO2006053571A2 (en) | 2006-05-26 |
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