ATE363491T1 - Reinigung von antikörpern durch protein a und ionenaustauschromatographie - Google Patents
Reinigung von antikörpern durch protein a und ionenaustauschromatographieInfo
- Publication number
- ATE363491T1 ATE363491T1 AT04715906T AT04715906T ATE363491T1 AT E363491 T1 ATE363491 T1 AT E363491T1 AT 04715906 T AT04715906 T AT 04715906T AT 04715906 T AT04715906 T AT 04715906T AT E363491 T1 ATE363491 T1 AT E363491T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- antibodies
- purification
- ion exchange
- exchange chromatography
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
- C07K17/02—Peptides being immobilised on, or in, an organic carrier
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0304576.2A GB0304576D0 (en) | 2003-02-28 | 2003-02-28 | Protein a chromatography |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE363491T1 true ATE363491T1 (de) | 2007-06-15 |
Family
ID=9953825
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT04715906T ATE363491T1 (de) | 2003-02-28 | 2004-03-01 | Reinigung von antikörpern durch protein a und ionenaustauschromatographie |
Country Status (9)
Country | Link |
---|---|
US (3) | US20060030696A1 (de) |
JP (2) | JP2007525412A (de) |
KR (1) | KR101200732B1 (de) |
CN (1) | CN100384874C (de) |
AT (1) | ATE363491T1 (de) |
DE (1) | DE602004006725T2 (de) |
ES (1) | ES2288252T3 (de) |
GB (1) | GB0304576D0 (de) |
TW (1) | TWI325428B (de) |
Families Citing this family (60)
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US20160279239A1 (en) | 2011-05-02 | 2016-09-29 | Immunomedics, Inc. | Subcutaneous administration of anti-cd74 antibody for systemic lupus erythematosus and autoimmune disease |
GB0220894D0 (en) * | 2002-09-09 | 2002-10-16 | Millipore Uk Ltd | Method of separation |
GB0304576D0 (en) * | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
US7479222B2 (en) * | 2004-02-05 | 2009-01-20 | Millipore Corporation | Porous adsorptive or chromatographic media |
EA200700751A1 (ru) * | 2004-10-05 | 2008-06-30 | Элан Фарма Интернэшнл Лимитед | Способы и композиции для улучшения продуцирования рекомбинантного белка |
US20160355591A1 (en) | 2011-05-02 | 2016-12-08 | Immunomedics, Inc. | Subcutaneous anti-hla-dr monoclonal antibody for treatment of hematologic malignancies |
US8067182B2 (en) * | 2005-03-11 | 2011-11-29 | Wyeth Llc | Method of weak partitioning chromatography |
EP1896504B1 (de) * | 2005-06-17 | 2012-11-21 | Wyeth LLC | VERFAHREN ZUR REINIGUNG FC-REGIONSHALTIGER ANTIKöRPER |
MY157846A (en) * | 2006-08-28 | 2016-07-29 | Ares Trading Sa | Process for the purification of fc-containing proteins |
BRPI0812288A2 (pt) | 2007-06-01 | 2014-11-25 | Hoffmann La Roche | Purificação de imunoglobulina. |
US9433922B2 (en) | 2007-08-14 | 2016-09-06 | Emd Millipore Corporation | Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same |
US20090130738A1 (en) * | 2007-11-19 | 2009-05-21 | Mikhail Kozlov | Media for membrane ion exchange chromatography |
US20090286283A1 (en) * | 2008-05-16 | 2009-11-19 | Samsung Electronics Co., Ltd. | Method and affinity column for purifying proteins |
US8592555B2 (en) | 2008-08-11 | 2013-11-26 | Emd Millipore Corporation | Immunoglobulin-binding proteins with improved specificity |
ES2391931T3 (es) * | 2008-09-15 | 2012-12-03 | Emd Millipore Corporation | Procedimientos para cuantificar la filtración de proteína desde resinas de cromatografía de afinidad basada en proteína |
EP2339339A4 (de) * | 2008-09-25 | 2016-10-12 | Jsr Corp | Füllmittel für affinitätschromatographie |
US10419541B2 (en) | 2008-11-26 | 2019-09-17 | Free Stream Media Corp. | Remotely control devices over a network without authentication or registration |
EP2360183B1 (de) | 2008-12-19 | 2016-07-27 | Takeda Pharmaceutical Company Limited | Antikörperreinigungsverfahren mittels "mixed-mode" chromatography unter verwendung einer arginine-haltigen beladungslösung |
AU2009331897A1 (en) | 2008-12-22 | 2010-07-01 | F. Hoffmann-La Roche Ag | Immunoglobulin purification |
SG162687A1 (en) * | 2008-12-24 | 2010-07-29 | Millipore Corp | Caustic stable chromatography ligands |
CN102712691A (zh) | 2009-08-06 | 2012-10-03 | 弗·哈夫曼-拉罗切有限公司 | 蛋白质纯化中提高病毒去除的方法 |
JP5791602B2 (ja) * | 2009-08-07 | 2015-10-07 | イー・エム・デイー・ミリポア・コーポレイシヨン | 試料中の1つ以上の不純物から標的タンパク質を精製するための方法 |
BR112012005869A2 (pt) * | 2009-09-15 | 2017-01-31 | Althea Tech Inc | cristais da proteína a, cristais reticulados e métodos de utilização dos mesmos |
MX2012004711A (es) | 2009-10-20 | 2012-05-23 | Abbott Lab | Aislamiento y purificacion de los anticuerpos anti-il-13 al usar cromatografia de afinidad con proteina a. |
ES2651065T3 (es) | 2009-12-18 | 2018-01-24 | Novartis Ag | Solución de lavado y método para cromatografía de afinidad |
WO2011090720A2 (en) * | 2009-12-29 | 2011-07-28 | Dr. Reddy's Laboratories Ltd | Purification of proteins |
US20130096284A1 (en) * | 2010-06-21 | 2013-04-18 | Kyowa Hakko Kirin Co., Ltd. | Method for purifying protein using amino acid |
MX2013004720A (es) * | 2010-11-01 | 2013-05-28 | Dsm Ip Assets Bv | Purificacion de anticuerpos mediante cromatografia de intercambio ionico de una uncia unidad. |
MX357821B (es) * | 2010-12-21 | 2018-07-25 | Hoffmann La Roche | Preparación de anticuerpo enriquecida con isoforma y método para obtenerla. |
AU2012250924B2 (en) | 2011-05-02 | 2017-05-25 | Immunomedics, Inc. | Ultrafiltration concentration of allotype selected antibodies for small-volume administration |
IL212911A0 (en) * | 2011-05-16 | 2011-07-31 | Omrix Biopharmaceuticals Ltd | Immunoglobulin reduced in thrombogenic contaminants and preparation thereof |
WO2012160536A1 (en) * | 2011-05-26 | 2012-11-29 | Dr Reddy's Laboratories Limited | Antibody purification |
SG10201604559TA (en) | 2011-06-08 | 2016-07-28 | Emd Millipore Corp | Chromatography matrices including novel staphylococcus aureus protein a based ligands |
DK2729482T3 (en) * | 2011-07-08 | 2018-05-07 | Merck Sharp & Dohme | PROCEDURE FOR CLEANING FC-FUSION PROTEIN |
WO2013028330A2 (en) | 2011-08-19 | 2013-02-28 | Emd Millipore Corporation | Methods of reducing level of one of more impurities in a sample during protein purification |
CN104066748A (zh) * | 2011-11-21 | 2014-09-24 | 霍夫曼-拉罗奇有限公司 | 抗c-met抗体的纯化 |
EP2791176B1 (de) * | 2011-12-15 | 2018-07-11 | Prestige Biopharma Pte. Ltd. | Verfahren zur antikörperreinigung |
EP2682168A1 (de) * | 2012-07-02 | 2014-01-08 | Millipore Corporation | Reinigung biologischer Moleküle |
CN103073616A (zh) * | 2013-01-06 | 2013-05-01 | 西北大学 | 使用混合模式层析技术去除抗体聚集体的方法 |
US9914770B2 (en) | 2013-04-30 | 2018-03-13 | Intas Pharmaceuticals Ltd | Cloning, expression and purification method for the preparation of ranibizumab |
CN105555795A (zh) * | 2013-09-17 | 2016-05-04 | 株式会社钟化 | 新抗体纯化方法和由该方法得到的抗体、以及使用了阳离子交换基团的新抗体纯化法和由该方法得到的抗体 |
KR102123700B1 (ko) * | 2013-12-17 | 2020-06-16 | (주)셀트리온 | 혼합 방식 크로마토그래피를 이용한 침출된 단백질 a 제거 방법 |
US10429359B2 (en) * | 2014-02-14 | 2019-10-01 | Ge Healthcare Bio-Sciences Ab | Automated multi-step purification system |
EP3118210B1 (de) * | 2014-03-11 | 2019-11-13 | Green Cross Holdings Corporation | Verfahren zur aufreinigung von immunglobulin |
EP3118209B1 (de) * | 2014-03-11 | 2020-02-19 | Green Cross Holdings Corporation | Verfahren zur aufreinigung von immunglobulin |
EP3116531B1 (de) | 2014-03-14 | 2021-10-20 | Merck Sharp & Dohme Corp. | Reinigen von insulin mit kationenaustausch- und umkehrphasenchromatografie in gegenwart eines organischen modifikators und erhöhter temperatur |
JP6451118B2 (ja) * | 2014-07-17 | 2019-01-16 | 東ソー株式会社 | 抗体の分離方法 |
KR101672947B1 (ko) | 2015-03-17 | 2016-11-04 | 주식회사 비에스엘 | 상황 알림 기능을 가진 히어링 디바이스 및 그에 의한 상황 알림 방법 |
US20160280767A1 (en) | 2015-03-23 | 2016-09-29 | Lonza Ltd. | Methods for controlling protein glycosylation |
AU2016288678B2 (en) | 2015-06-30 | 2021-05-27 | Vapotherm, Inc. | Nasal cannula for continuous and simultaneous delivery of aerosolized medicament and high flow therapy |
US11186858B1 (en) | 2016-03-15 | 2021-11-30 | Fresenius Kabi Deutschland Gmbh | Methods for increasing biosimilarity |
EP3487868A4 (de) | 2016-07-25 | 2020-07-29 | Cephalon, Inc. | Waschpuffer für affinitätschromatografie |
CN106380519B (zh) * | 2016-10-17 | 2019-11-01 | 深圳万乐药业有限公司 | 一种单克隆抗体的纯化方法 |
EP3606964A4 (de) | 2017-04-03 | 2020-12-09 | Immunomedics, Inc. | Subkutane verabreichung von antikörper-arzneimittelkonjugaten zur krebstherapie |
US11724056B2 (en) | 2017-09-08 | 2023-08-15 | Vapotherm, Inc. | Birfurcated cannula device |
US11155575B2 (en) | 2018-03-21 | 2021-10-26 | Waters Technologies Corporation | Non-antibody high-affinity-based sample preparation, sorbent, devices and methods |
JP7512891B2 (ja) * | 2018-06-29 | 2024-07-09 | 三菱ケミカル株式会社 | ポリペプチドの分離方法、ポリペプチドの製造方法及びポリペプチドの精製装置 |
CN109324143B (zh) * | 2018-10-19 | 2021-10-29 | 张骐 | 利用二维液相分离制备抗体类产品相关杂质的方法 |
AU2020302871A1 (en) | 2019-06-28 | 2022-01-27 | Vapotherm, Inc. | Variable geometry cannula |
CN114728147A (zh) | 2019-09-26 | 2022-07-08 | 蒸汽热能公司 | 插管内部安装的雾化器 |
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CA1201063A (en) | 1982-07-20 | 1986-02-25 | Winnipeg Rh Institute Inc., (The) | Process for preparing purified immune globulin (igg) |
US5089605A (en) | 1987-03-13 | 1992-02-18 | Repligen Corporation | Immobilized immunoglobulin-binding proteins |
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US5118796A (en) * | 1987-12-09 | 1992-06-02 | Centocor, Incorporated | Efficient large-scale purification of immunoglobulins and derivatives |
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US5115101A (en) * | 1988-06-08 | 1992-05-19 | Miles Inc. | Removal of protein A from antibody preparations |
US5650319A (en) * | 1990-04-20 | 1997-07-22 | Teijin Limited | Human monoclonal antibody to glycoprotein GPIII of varicella zoster virus |
EP0481089B1 (de) | 1990-04-20 | 1997-07-30 | Teijin Limited | Menschliche monoklonale antikörper gegen gpiii des varizella-zoster-virus |
GB9022547D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Purified immunoglobulin |
WO1992018629A1 (en) | 1991-04-19 | 1992-10-29 | Genetics Institute, Inc. | Recombinant 3f8-type antibodies |
DK0669836T3 (da) * | 1992-11-13 | 1996-10-14 | Idec Pharma Corp | Terapeutisk anvendelse af kimære og radioaktivt mærkede antistoffer og humant B-lymfocytbegrænset differentieringsantigen til behandling af B-cellelymfom |
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JP2001502895A (ja) * | 1996-09-20 | 2001-03-06 | ザ・ジェネラル・ホスピタル・コーポレイション | アルファ―2―抗プラスミンに対するキメラ、ヒト化および一本鎖抗体 |
DK0998486T4 (da) | 1997-06-13 | 2012-05-14 | Genentech Inc | Proteinoprensning ved kromatografi efterfulgt af filtrering på en ladet fase |
US5886154A (en) * | 1997-06-20 | 1999-03-23 | Lebing; Wytold R. | Chromatographic method for high yield purification and viral inactivation of antibodies |
TW505655B (en) * | 1997-10-14 | 2002-10-11 | Tanox Inc | Enhanced aggregate removal from bulk-biologicals using ion exchange chromatography |
US6504013B1 (en) | 2000-02-01 | 2003-01-07 | Idexx Laboratories, Inc. | Canine allergy therapeutic recombinant chimeric anti-IgE monoclonal antibody |
BR9909789A (pt) * | 1998-04-22 | 2000-12-26 | Genvec Inc | Método de purificação eficiente de adenovìrus |
DK1084136T3 (da) * | 1998-06-01 | 2005-01-03 | Genentech Inc | Adskillelse af monomere af et antistof fra dets multimere ved anvendelse af ionbytterchromatografi |
GB9902000D0 (en) | 1999-01-30 | 1999-03-17 | Delta Biotechnology Ltd | Process |
AU2002327164A1 (en) * | 2001-01-29 | 2002-12-09 | Idec Pharmaceuticals Corporation | Engineered tetravalent antibodies and methods of use |
PL374311A1 (en) * | 2001-12-21 | 2005-10-03 | Immunex Corporation | Methods for purifying protein |
EP1501369B1 (de) * | 2002-04-26 | 2015-06-24 | Genentech, Inc. | Nichtaffinitätsreinigung von proteinen |
GB0304576D0 (en) * | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
AU2004215653B2 (en) | 2003-02-28 | 2011-03-17 | Lonza Biologics Plc. | Antibody purification by protein A and ion exchange chromatography |
JPWO2004087761A1 (ja) * | 2003-03-31 | 2006-07-27 | 麒麟麦酒株式会社 | ヒトモノクローナル抗体およびヒトポリクローナル抗体の精製 |
WO2005044856A2 (en) * | 2003-10-27 | 2005-05-19 | Wyeth | Removal of high molecular weight aggregates using hydroxyapatite chromatography |
KR20070072510A (ko) * | 2004-08-30 | 2007-07-04 | 론자 바이올로직스 피엘씨 | 항체 정제를 위한 친화도- 및 이온 교환- 크로마토그래피 |
-
2003
- 2003-02-28 GB GBGB0304576.2A patent/GB0304576D0/en not_active Ceased
-
2004
- 2004-03-01 ES ES04715906T patent/ES2288252T3/es not_active Expired - Lifetime
- 2004-03-01 KR KR1020057015912A patent/KR101200732B1/ko not_active IP Right Cessation
- 2004-03-01 AT AT04715906T patent/ATE363491T1/de active
- 2004-03-01 CN CNB2004800093477A patent/CN100384874C/zh not_active Expired - Lifetime
- 2004-03-01 JP JP2006501980A patent/JP2007525412A/ja not_active Withdrawn
- 2004-03-01 DE DE602004006725T patent/DE602004006725T2/de not_active Expired - Lifetime
- 2004-03-11 TW TW093106464A patent/TWI325428B/zh not_active IP Right Cessation
-
2005
- 2005-08-25 US US11/210,669 patent/US20060030696A1/en not_active Abandoned
-
2006
- 2006-05-19 US US11/419,306 patent/US7847071B2/en not_active Expired - Lifetime
-
2010
- 2010-10-22 US US12/910,301 patent/US20110040075A1/en not_active Abandoned
-
2011
- 2011-10-21 JP JP2011231797A patent/JP5752558B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US20060030696A1 (en) | 2006-02-09 |
KR20050113617A (ko) | 2005-12-02 |
DE602004006725D1 (de) | 2007-07-12 |
US20110040075A1 (en) | 2011-02-17 |
US7847071B2 (en) | 2010-12-07 |
JP5752558B2 (ja) | 2015-07-22 |
JP2012067108A (ja) | 2012-04-05 |
DE602004006725T2 (de) | 2008-02-07 |
CN1771260A (zh) | 2006-05-10 |
US20060194953A1 (en) | 2006-08-31 |
JP2007525412A (ja) | 2007-09-06 |
GB0304576D0 (en) | 2003-04-02 |
TWI325428B (en) | 2010-06-01 |
KR101200732B1 (ko) | 2012-11-13 |
CN100384874C (zh) | 2008-04-30 |
TW200530265A (en) | 2005-09-16 |
ES2288252T3 (es) | 2008-01-01 |
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