BR9909789A - Método de purificação eficiente de adenovìrus - Google Patents
Método de purificação eficiente de adenovìrusInfo
- Publication number
- BR9909789A BR9909789A BR9909789-3A BR9909789A BR9909789A BR 9909789 A BR9909789 A BR 9909789A BR 9909789 A BR9909789 A BR 9909789A BR 9909789 A BR9909789 A BR 9909789A
- Authority
- BR
- Brazil
- Prior art keywords
- adenovirus
- solution
- chromatography resin
- purification method
- sample solution
- Prior art date
Links
- 241000701161 unidentified adenovirus Species 0.000 title abstract 13
- 238000000034 method Methods 0.000 title abstract 6
- 238000000746 purification Methods 0.000 title abstract 3
- 239000012539 chromatography resin Substances 0.000 abstract 5
- -1 dimethylaminopropyl Chemical group 0.000 abstract 4
- 239000012488 sample solution Substances 0.000 abstract 3
- 239000000243 solution Substances 0.000 abstract 3
- 238000005571 anion exchange chromatography Methods 0.000 abstract 2
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 abstract 2
- 238000010828 elution Methods 0.000 abstract 2
- 239000002245 particle Substances 0.000 abstract 2
- 238000002835 absorbance Methods 0.000 abstract 1
- 230000006037 cell lysis Effects 0.000 abstract 1
- 230000009089 cytolysis Effects 0.000 abstract 1
- 239000011259 mixed solution Substances 0.000 abstract 1
- 238000011002 quantification Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/20—Anion exchangers for chromatographic processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/34—Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
- B01D15/345—Perfusive chromatography
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10351—Methods of production or purification of viral material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N2030/621—Detectors specially adapted therefor signal-to-noise ratio
- G01N2030/625—Detectors specially adapted therefor signal-to-noise ratio by measuring reference material, e.g. carrier without sample
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/01—DNA viruses
- G01N2333/075—Adenoviridae
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
Abstract
<B> MéTODO DE PURIFICAçãO EFICIENTE DE ADENOVìRUS <D> Um método de enriquecimento de uma solução de um adenovírus compreendendo a plicação de uma solução misturada compreendendo adenovírus e pelo menos um tipo indesejável de biomolécula a uma resina de cromatografia de troca aniónica contendo uma porção de ligação selecionada do grupo que consiste em dimetilaminopropil, dimetilaminobutil, dimetilaminoisobutil, e dimetilamino pentil e eluição do adenovírus a partir da resina de cromatografia. Também é fornecido um método de purificação de adenovírus de células infectadas por adenovírus compreendendo a lise de tais células, aplicação do produto de lise de célula a uma resina de cromatografia única, eluindo o adenovírus da resina de cromatografia, e coletando uma fração contendo adenovírus que é substancialmente tão pura quanto o adenovírus purificado por gradiente de densidade de CsCl triplo. O presente método também apresenta um método de quantificar de forma precisa o número de partículas adenovirais em uma solução de adenovírus compreendendo a aplicação e eluição de uma resina de cromatografia de troca aniónica uma solução de amostra de adenovírus, comparando a bsorvência da solução de amostra de adenovírus e a absorbância de uma solução padrão de adenovírus, e quantificação do número de partículas adenovirais na solução de amostra.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US8262898P | 1998-04-22 | 1998-04-22 | |
PCT/US1999/008843 WO1999054441A1 (en) | 1998-04-22 | 1999-04-22 | Efficient purification of adenovirus |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9909789A true BR9909789A (pt) | 2000-12-26 |
Family
ID=22172352
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9909789-3A BR9909789A (pt) | 1998-04-22 | 1999-04-22 | Método de purificação eficiente de adenovìrus |
Country Status (20)
Country | Link |
---|---|
US (3) | US6383795B1 (pt) |
EP (1) | EP1073721B1 (pt) |
JP (1) | JP2002512361A (pt) |
AT (1) | ATE303434T1 (pt) |
AU (1) | AU756889B2 (pt) |
BG (1) | BG104928A (pt) |
BR (1) | BR9909789A (pt) |
CA (1) | CA2328462C (pt) |
DE (1) | DE69927013T2 (pt) |
EA (1) | EA200001096A1 (pt) |
EE (1) | EE200000605A (pt) |
HU (1) | HUP0102209A2 (pt) |
IL (1) | IL139040A0 (pt) |
IS (1) | IS5673A (pt) |
MX (1) | MXPA00010338A (pt) |
NO (1) | NO20005295L (pt) |
PL (1) | PL343630A1 (pt) |
SK (1) | SK15682000A3 (pt) |
WO (1) | WO1999054441A1 (pt) |
ZA (1) | ZA200005363B (pt) |
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US7732129B1 (en) * | 1998-12-01 | 2010-06-08 | Crucell Holland B.V. | Method for the production and purification of adenoviral vectors |
JP4492826B2 (ja) | 1996-11-20 | 2010-06-30 | イントロジェン セラピューティクス,インコーポレイテッド | アデノウイルスベクターの産生および精製のための改良された方法 |
US6689600B1 (en) | 1998-11-16 | 2004-02-10 | Introgen Therapeutics, Inc. | Formulation of adenovirus for gene therapy |
EP1155120B1 (fr) | 1999-02-22 | 2006-07-05 | Transgene S.A. | Procede d'obtention d'une preparation virale purifiee |
GB9915413D0 (en) * | 1999-07-01 | 1999-09-01 | Glaxo Group Ltd | Propagation method |
US6447995B1 (en) | 2000-10-04 | 2002-09-10 | Genvec, Inc. | Utilizing intrinsic fluorescence to detect adenovirus |
US7754208B2 (en) | 2001-01-17 | 2010-07-13 | Trubion Pharmaceuticals, Inc. | Binding domain-immunoglobulin fusion proteins |
US7829084B2 (en) | 2001-01-17 | 2010-11-09 | Trubion Pharmaceuticals, Inc. | Binding constructs and methods for use thereof |
JP3547715B2 (ja) * | 2001-03-06 | 2004-07-28 | 榮 新垣 | ベクター精製用装置及び方法 |
CA2455499C (en) * | 2001-08-08 | 2011-06-21 | The Trustees Of The University Of Pennsylvania | Method for purification of viral vectors having proteins which bind sialic acid |
EP1371723A1 (en) * | 2002-06-12 | 2003-12-17 | Procorde GmbH | Process for preparing an adenovirus-containing preparation |
US20040248798A1 (en) | 2003-02-14 | 2004-12-09 | Peter Sutovsky | Contraceptive methods and compositions related to proteasomal interference |
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CN1816619A (zh) * | 2003-06-18 | 2006-08-09 | 昂尼克斯药物公司 | 纯化病毒的方法 |
WO2005012537A2 (en) * | 2003-07-25 | 2005-02-10 | Genvec, Inc. | Adenoviral vector-based vaccines |
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ATE435906T1 (de) * | 2004-02-23 | 2009-07-15 | Crucell Holland Bv | Verfahren zur reinigung von viren |
JP2007532656A (ja) | 2004-04-12 | 2007-11-15 | アメリカ合衆国 | アデノウイルスベクターを用いて免疫応答を誘導するための方法 |
GB0411081D0 (en) * | 2004-05-18 | 2004-06-23 | Glaxo Group Ltd | Novel apparatus |
EP1784493A2 (en) * | 2004-09-01 | 2007-05-16 | The Government of the United States of America as Represented by The Department of Health and Human Services | Adenoviral vectors able to transduce apcs, potential use in immune response generation |
CA2586107A1 (en) | 2004-11-03 | 2006-05-18 | Introgen Therapeutics Inc. | A novel method for the production and purification of adenoviral vectors |
JP4626350B2 (ja) * | 2005-03-22 | 2011-02-09 | ソニー株式会社 | ハイブリダイゼーション検出に適する反応部などを有する流路系、該流路系を用いるハイブリダイゼーション検出装置 |
DK2567976T3 (en) | 2005-03-23 | 2017-10-23 | Genmab As | ANTIBODIES AGAINST CD38 FOR TREATMENT OF MULTIPLE MYLOMA |
DK1869171T4 (en) * | 2005-04-11 | 2016-01-18 | Crucell Holland Bv | Virus cleaning using ultrafiltration |
PL1912675T3 (pl) | 2005-07-25 | 2014-10-31 | Emergent Product Dev Seattle | Zmniejszanie liczby komórek B za pomocą cząsteczek wiążących swoistych dla antygenów CD37 i CD20 |
BRPI0618441B8 (pt) * | 2005-11-10 | 2021-07-27 | Genvec Inc | vetor adenoviral |
EP1808697A1 (en) * | 2006-01-13 | 2007-07-18 | Novartis Vaccines and Diagnostics, Inc. | Use of an ion exchange matrix for determining the concentration of virus particles and/or virus antigens |
NZ596865A (en) | 2006-06-12 | 2013-07-26 | Emergent Product Dev Seattle | Single-chain multivalent binding proteins with effector function |
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EP2385065A1 (en) | 2007-11-01 | 2011-11-09 | Perseid Therapeutics LLC | Immunosuppressive polypeptides and nucleic acids |
CN102099377A (zh) * | 2008-04-11 | 2011-06-15 | 新兴产品开发西雅图有限公司 | Cd37免疫治疗剂及其与双功能化学治疗剂的联合 |
US10041049B2 (en) * | 2008-11-03 | 2018-08-07 | Janssen Vaccines & Prevention B.V. | Method for the production of adenoviral vectors |
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CA2786835C (en) | 2010-02-15 | 2021-08-31 | Crucell Holland B.V. | Method for the production of ad26 adenoviral vectors |
JP2013527753A (ja) | 2010-03-23 | 2013-07-04 | イントレキソン コーポレーション | 治療タンパク質を条件的に発現するベクター、該ベクターを含む宿主細胞およびそれらの使用 |
JP6189754B2 (ja) | 2011-03-04 | 2017-08-30 | イントレキソン コーポレーション | タンパク質を条件的に発現するベクター |
CN102590431B (zh) * | 2012-02-10 | 2014-04-02 | 济南康众医药科技开发有限公司 | 一种治疗咳嗽的中药药物组合物检测方法 |
MX2017002934A (es) | 2014-09-07 | 2017-05-30 | Selecta Biosciences Inc | Metodos y composiciones para atenuar las respuestas inmunes anti-vector de transferencia viral que modula la expresion de genes. |
TWI710635B (zh) | 2014-10-09 | 2020-11-21 | 美商珍維克公司 | 編碼人類無調同源物-1(hath1)之腺病毒載體 |
CN108367004B (zh) | 2015-09-21 | 2022-09-13 | 阿帕特夫研究和发展有限公司 | Cd3结合多肽 |
AU2018205496A1 (en) | 2017-01-07 | 2019-07-25 | Selecta Biosciences, Inc. | Patterned dosing of immunosuppressants coupled to synthetic nanocarriers |
AU2018347583A1 (en) | 2017-10-13 | 2020-05-21 | Selecta Biosciences, Inc. | Methods and compositions for attenuating anti-viral transfer vector IgM responses |
GB201806736D0 (en) * | 2018-04-25 | 2018-06-06 | Ge Healthcare Bioprocess R&D Ab | Method for virus purification |
WO2020172509A1 (en) | 2019-02-21 | 2020-08-27 | Unleash Immuno Oncolytics, Inc. | Oncolytic adenoviral vector and methods of use |
MX2021013163A (es) | 2019-04-28 | 2022-02-21 | Selecta Biosciences Inc | Métodos para el tratamiento de sujetos con inmunidad preexistente a vectores de transferencia viral. |
CA3141863A1 (en) | 2019-05-28 | 2020-12-03 | Selecta Biosciences, Inc. | Methods and compositions for attenuated anti-viral transfer vector immune response |
US11725192B2 (en) * | 2019-07-12 | 2023-08-15 | Sangamo Therapeutics, Inc. | Separation and quantification of empty and full viral capsid particles |
US20230141563A1 (en) | 2021-10-12 | 2023-05-11 | Selecta Biosciences, Inc. | Methods and compositions for attenuating anti-viral transfer vector igm responses |
US20230357437A1 (en) | 2022-03-09 | 2023-11-09 | Selecta Biosciences, Inc. | Immunosuppressants in combination with anti-igm agents and related dosing |
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-
1999
- 1999-04-22 IL IL13904099A patent/IL139040A0/xx unknown
- 1999-04-22 MX MXPA00010338A patent/MXPA00010338A/es unknown
- 1999-04-22 EP EP99918788A patent/EP1073721B1/en not_active Expired - Lifetime
- 1999-04-22 BR BR9909789-3A patent/BR9909789A/pt not_active Application Discontinuation
- 1999-04-22 HU HU0102209A patent/HUP0102209A2/hu unknown
- 1999-04-22 EE EEP200000605A patent/EE200000605A/xx unknown
- 1999-04-22 AT AT99918788T patent/ATE303434T1/de not_active IP Right Cessation
- 1999-04-22 SK SK1568-2000A patent/SK15682000A3/sk unknown
- 1999-04-22 DE DE69927013T patent/DE69927013T2/de not_active Expired - Lifetime
- 1999-04-22 EA EA200001096A patent/EA200001096A1/ru unknown
- 1999-04-22 JP JP2000544773A patent/JP2002512361A/ja active Pending
- 1999-04-22 AU AU36620/99A patent/AU756889B2/en not_active Ceased
- 1999-04-22 CA CA2328462A patent/CA2328462C/en not_active Expired - Lifetime
- 1999-04-22 US US09/296,962 patent/US6383795B1/en not_active Expired - Lifetime
- 1999-04-22 WO PCT/US1999/008843 patent/WO1999054441A1/en active IP Right Grant
- 1999-04-22 PL PL99343630A patent/PL343630A1/xx unknown
-
2000
- 2000-10-03 ZA ZA200005363A patent/ZA200005363B/xx unknown
- 2000-10-20 NO NO20005295A patent/NO20005295L/no not_active Application Discontinuation
- 2000-10-20 IS IS5673A patent/IS5673A/is unknown
- 2000-11-08 BG BG104928A patent/BG104928A/xx unknown
-
2001
- 2001-11-30 US US09/997,909 patent/US6586226B2/en not_active Expired - Lifetime
-
2003
- 2003-05-05 US US10/429,303 patent/US7141406B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
WO1999054441A8 (en) | 2000-02-24 |
EA200001096A1 (ru) | 2001-04-23 |
ATE303434T1 (de) | 2005-09-15 |
US20030203469A1 (en) | 2003-10-30 |
HUP0102209A2 (hu) | 2001-09-28 |
NO20005295L (no) | 2000-11-30 |
PL343630A1 (en) | 2001-08-27 |
IL139040A0 (en) | 2001-11-25 |
US6586226B2 (en) | 2003-07-01 |
IS5673A (is) | 2000-10-20 |
US7141406B2 (en) | 2006-11-28 |
WO1999054441A1 (en) | 1999-10-28 |
AU3662099A (en) | 1999-11-08 |
SK15682000A3 (sk) | 2001-05-10 |
BG104928A (en) | 2001-09-28 |
EP1073721A1 (en) | 2001-02-07 |
EE200000605A (et) | 2002-04-15 |
CA2328462A1 (en) | 1999-10-28 |
JP2002512361A (ja) | 2002-04-23 |
AU756889B2 (en) | 2003-01-23 |
US6383795B1 (en) | 2002-05-07 |
EP1073721B1 (en) | 2005-08-31 |
CA2328462C (en) | 2010-11-09 |
DE69927013T2 (de) | 2006-06-29 |
ZA200005363B (en) | 2001-09-25 |
NO20005295D0 (no) | 2000-10-20 |
US20020034735A1 (en) | 2002-03-21 |
DE69927013D1 (de) | 2005-10-06 |
MXPA00010338A (es) | 2005-06-17 |
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