ATE348146T1 - Sekretion von proteinen mit mehreren disulfidbindungen in bakterien und verwendungen davon - Google Patents

Sekretion von proteinen mit mehreren disulfidbindungen in bakterien und verwendungen davon

Info

Publication number
ATE348146T1
ATE348146T1 AT03716803T AT03716803T ATE348146T1 AT E348146 T1 ATE348146 T1 AT E348146T1 AT 03716803 T AT03716803 T AT 03716803T AT 03716803 T AT03716803 T AT 03716803T AT E348146 T1 ATE348146 T1 AT E348146T1
Authority
AT
Austria
Prior art keywords
secretion
bacteria
disulfide bonds
proteins
multiple disulfide
Prior art date
Application number
AT03716803T
Other languages
English (en)
Inventor
George Georgiou
Lluis Masip
Matthew Delisa
Original Assignee
Res Dev Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Res Dev Foundation filed Critical Res Dev Foundation
Application granted granted Critical
Publication of ATE348146T1 publication Critical patent/ATE348146T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT03716803T 2002-03-23 2003-03-24 Sekretion von proteinen mit mehreren disulfidbindungen in bakterien und verwendungen davon ATE348146T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US36713002P 2002-03-23 2002-03-23

Publications (1)

Publication Number Publication Date
ATE348146T1 true ATE348146T1 (de) 2007-01-15

Family

ID=28675326

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03716803T ATE348146T1 (de) 2002-03-23 2003-03-24 Sekretion von proteinen mit mehreren disulfidbindungen in bakterien und verwendungen davon

Country Status (9)

Country Link
US (2) US20030219870A1 (de)
EP (1) EP1487966B1 (de)
JP (1) JP2005520569A (de)
CN (1) CN100516194C (de)
AT (1) ATE348146T1 (de)
AU (1) AU2003220493B2 (de)
CA (1) CA2480159A1 (de)
DE (1) DE60310385T2 (de)
WO (1) WO2003083056A2 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10256669B4 (de) * 2002-12-04 2006-03-09 Universitätsklinikum Charité an der Humboldt-Universität zu Berlin Technologietransferstelle Gemisch mindestens zweier Fusionsproteine sowie ihre Herstellung und Verwendung
EP1708741B1 (de) * 2003-12-24 2016-03-30 Aduro Biotech Fusionsproteine kodierende rekombinante nucleinsäure-moleküle mit antigenen und bakteriellen sekretorischen signal-polypeptiden, expressionskassetten und bakterien und anwendungsverfahren dafür
US7842289B2 (en) 2003-12-24 2010-11-30 Aduro Biotech Recombinant nucleic acid molecules, expression cassettes, and bacteria, and methods of use thereof
EP1748077B1 (de) * 2004-04-20 2013-02-27 Ajinomoto Co., Inc. Verfahren zur proteinherstellung
JP5235657B2 (ja) * 2005-03-24 2013-07-10 バイオジェネリックス ゲーエムベーハー 原核生物における可溶性活性真核生物グリコシルトランスフェラーゼの発現
US20070026012A1 (en) 2005-08-01 2007-02-01 Cornell Research Foundation, Inc. Compositions and methods for monitoring and altering protein folding and solubility
WO2007024877A2 (en) * 2005-08-22 2007-03-01 Cornell Research Foundation, Inc. Compositions and methods for analyzing protein interactions
KR100742218B1 (ko) 2005-10-21 2007-07-24 학교법인 포항공과대학교 트윈 아르기닌 트랜스로케이션 분비경로를 가지는오가노포스포러스 하이드롤라제 분비서열을 이용한대장균에서의 목적 외래 단백질 분비
US7935804B2 (en) 2006-03-01 2011-05-03 Aduro Biotech Engineered Listeria and methods of use thereof
DK2102239T3 (da) 2006-11-30 2012-05-29 Res Dev Foundation Forbedrede immunoglobulin-biblioteker
US8722584B2 (en) * 2007-01-12 2014-05-13 Cornell University Genetic selection for protein folding and solubility in the bacterial periplasm
WO2008137475A2 (en) 2007-05-01 2008-11-13 Research Development Foundation Immunoglobulin fc libraries
CN101932591B (zh) 2007-07-09 2018-02-16 健泰科生物技术公司 在多肽的重组生产期间防止二硫键还原
CA2766065C (en) * 2009-06-30 2020-07-21 Research Development Foundation Immunoglobulin fc polypeptides
BR112012021070B1 (pt) * 2010-02-23 2020-02-27 Toray Industries Inc. Método para produzir cadaverina
CA2793424A1 (en) * 2010-03-18 2011-09-22 Cornell University Engineering correctly folded antibodies using inner membrane display of twin-arginine translocation intermediates
WO2012109133A1 (en) 2011-02-07 2012-08-16 Research Development Foundation Engineered immunoglobulin fc polypeptides
GB201419899D0 (en) * 2014-11-07 2014-12-24 Univ Kent Canterbury Biopharmaceutical production method
US10457737B2 (en) 2015-02-09 2019-10-29 Research Development Foundation Engineered immunoglobulin Fc polypeptides displaying improved complement activation
WO2017040380A2 (en) 2015-08-28 2017-03-09 Research Development Foundation Engineered antibody fc variants
EP3665195A4 (de) 2017-08-11 2021-05-19 Research Development Foundation Manipulierte antikörper-fc-varianten für erhöhte serumhalbwertszeit
FI3877552T3 (fi) * 2018-11-08 2023-05-11 Sutro Biopharma Inc E coli -kantoja, joilla on oksidativinen sytoplasma
CN110616227A (zh) * 2019-09-30 2019-12-27 天津科技大学 一种来源于黄粉虫的抗冻蛋白的基因、重组表达载体、工程菌株和应用
WO2023239940A1 (en) 2022-06-10 2023-12-14 Research Development Foundation Engineered fcriib selective igg1 fc variants and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19513676A1 (de) * 1995-04-11 1996-10-17 Behringwerke Ag Cytoplasmatische Expression von Antikörpern, Antikörperfragmenten und Antikörperfragmentfusionsmolekülen in E.coli
US6277375B1 (en) * 1997-03-03 2001-08-21 Board Of Regents, The University Of Texas System Immunoglobulin-like domains with increased half-lives
US6022952A (en) * 1998-04-01 2000-02-08 University Of Alberta Compositions and methods for protein secretion
AU2001292749A1 (en) * 2000-09-18 2002-03-26 Genencor International, Inc. Twin-arginine translocation in bacillus
EP1354056B1 (de) * 2000-10-10 2014-11-26 Danisco US Inc. Erhöhte polypeptidsekretion von einem mikroorganismus
US7419783B2 (en) * 2001-11-05 2008-09-02 Research Development Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria

Also Published As

Publication number Publication date
CA2480159A1 (en) 2003-10-09
DE60310385T2 (de) 2007-09-20
US20030219870A1 (en) 2003-11-27
DE60310385D1 (de) 2007-01-25
WO2003083056A2 (en) 2003-10-09
WO2003083056A3 (en) 2004-07-15
US20110046011A1 (en) 2011-02-24
CN1656213A (zh) 2005-08-17
CN100516194C (zh) 2009-07-22
AU2003220493B2 (en) 2008-06-05
AU2003220493A1 (en) 2003-10-13
JP2005520569A (ja) 2005-07-14
EP1487966A2 (de) 2004-12-22
EP1487966A4 (de) 2005-11-23
EP1487966B1 (de) 2006-12-13

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