AR114547A1 - Microorganismos con producción mejorada de 1,3-propanodiol y ácido butírico - Google Patents

Microorganismos con producción mejorada de 1,3-propanodiol y ácido butírico

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Publication number
AR114547A1
AR114547A1 ARP190102267A ARP190102267A AR114547A1 AR 114547 A1 AR114547 A1 AR 114547A1 AR P190102267 A ARP190102267 A AR P190102267A AR P190102267 A ARP190102267 A AR P190102267A AR 114547 A1 AR114547 A1 AR 114547A1
Authority
AR
Argentina
Prior art keywords
mutant strain
clostridium
propanodiol
microorganisms
butyric acid
Prior art date
Application number
ARP190102267A
Other languages
English (en)
Original Assignee
Metabolic Explorer Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Publication of AR114547A1 publication Critical patent/AR114547A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0067—Oxidoreductases (1.) acting on hydrogen as donor (1.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y112/00—Oxidoreductases acting on hydrogen as donor (1.12)
    • C12Y112/07—Oxidoreductases acting on hydrogen as donor (1.12) with an iron-sulfur protein as acceptor (1.12.7)
    • C12Y112/07002—Ferredoxin hydrogenase (1.12.7.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/0103—Glycerol kinase (2.7.1.30)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente se refiere a una cepa mutante de Clostridium acetobutylicum que comprende actividad atenuada de glicerol quinasa, Además, se refiere a un consorcio de Clostridium que comprende por lo menos dicha cepa mutante y por lo menos otra especie de Clostridium elegida entre C. sporogenes y C. sphenoides. Como esta cepa modificada puede adaptarse para el crecimiento y para la producción de 1,3-propanodiol en un medio de cultivo apropiado con alto contenido de glicerol, la presente además se refiere a un método para la producción de 1,3-propanodiol y ácido butírico, mediante el cultivo de al menos esta cepa mutante en un medio de cultivo apropiado. Reivindicación 1: Una cepa mutante de Clostridium acetobutylicum que expresa los genes dhaB1, dhaB2 y dhaT de C. butyricum y que comprende actividad atenuada de glicerol quinasa.
ARP190102267A 2018-08-10 2019-08-09 Microorganismos con producción mejorada de 1,3-propanodiol y ácido butírico AR114547A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP18306099 2018-08-10

Publications (1)

Publication Number Publication Date
AR114547A1 true AR114547A1 (es) 2020-09-16

Family

ID=63449410

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP190102267A AR114547A1 (es) 2018-08-10 2019-08-09 Microorganismos con producción mejorada de 1,3-propanodiol y ácido butírico

Country Status (13)

Country Link
US (1) US11814663B2 (es)
EP (1) EP3833770A1 (es)
JP (1) JP2021533764A (es)
KR (1) KR20210043606A (es)
CN (1) CN112567044A (es)
AR (1) AR114547A1 (es)
AU (1) AU2019319045A1 (es)
BR (1) BR112021002573A2 (es)
CA (1) CA3107573A1 (es)
IL (1) IL280749A (es)
MX (1) MX2021001619A (es)
SG (1) SG11202101283WA (es)
WO (1) WO2020030775A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT202000031979A1 (it) 2020-12-22 2022-06-22 Novamont Spa Processo di purificazione di una miscela comprendente dioli e acetali
IT202100006419A1 (it) 2021-03-17 2022-09-17 Novamont Spa Recupero di dioli da una miscela

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2633939B1 (fr) 1988-07-06 1991-05-31 Inst Francais Du Petrole Procede de production d'acide butyrique normal par fermentation a partir d'un substrat sucre en presence d'au moins une souche de l'espece clostridium
FR2796081B1 (fr) 1999-07-09 2003-09-26 Agronomique Inst Nat Rech Procede de preparation du 1,3-propanediol par un micro-organisme recombinant en l'absence de coenzyme b12 ou de l'un de ses precurseurs
WO2002077183A2 (en) * 2001-03-21 2002-10-03 Elitra Pharmaceuticals, Inc. Identification of essential genes in microorganisms
ES2356526T3 (es) 2002-10-04 2011-04-08 E.I. Du Pont De Nemours And Company Procedimiento para la producción biológica de 1,3-propanodiol con elevado rendimiento.
CN1327001C (zh) 2005-06-03 2007-07-18 清华大学 利用生物柴油副产物甘油生产1,3－丙二醇的方法
JP5203375B2 (ja) 2006-10-03 2013-06-05 メタボリック エクスプローラー クロストリジウム菌における染色体取り込みおよびｄｎａ配列置換法
EP2400027A1 (en) * 2006-10-31 2011-12-28 Metabolic Explorer Process for the biological production of 1,3-propanediol from glycerol with high yield
BRPI0814640A2 (pt) 2007-07-23 2014-10-14 Dsm Ip Assets Bv Processo para a preparação de butanol e etanol
CN101925567B (zh) 2007-11-30 2014-04-23 代谢探索者公司 用于从发酵液纯化醇的方法
MX2011003558A (es) 2008-10-03 2011-05-02 Metabolic Explorer Sa Metodo para purificar un alcohol de un caldo de fermentacion usando un evaporador de pelicula descendente, un evaporador de pelicula enjuagada, un evaporador de pelicula delgada o un evaporador de trayectoria corta.
EP2248904A1 (en) 2009-05-05 2010-11-10 Metabolic Explorer Continuous culture for 1,3-propanediol production using high glycerine concentration
WO2011042434A1 (en) 2009-10-05 2011-04-14 Metabolic Explorer 1,3-propanediol production with immobilised cells reactors
AR083799A1 (es) * 2010-11-10 2013-03-20 Metabolic Explorer Sa Microorganismos para la produccion de 1,3-propanodiol usando concentracion de glicerina alta
GB201116971D0 (en) * 2011-10-03 2011-11-16 Isis Innovation Cofactor regeneration system
KR20140032057A (ko) * 2012-09-05 2014-03-14 삼성전자주식회사 글리세롤 탈수 반응 산물의 생산 능력이 향상된 재조합 미생물 및 그 용도
WO2016042160A1 (en) * 2014-09-18 2016-03-24 Institut National Des Sciences Appliquees Clostridium acetobutylicum strains unable to produce hydrogen and useful for the continuous production of chemicals and fuels
FR3039167B1 (fr) 2015-07-23 2023-11-10 Afyren Procede d'extraction de metabolites fermentaires produits par fermentation anaerobie a partir de biomasse fermentescible
DK3583221T3 (da) * 2017-02-20 2021-09-06 Metabolic Explorer Sa Mikrobielt konsortium omfattende clostridia til fremstilling af 1,3-propandiol ud fra glycerol

Also Published As

Publication number Publication date
AU2019319045A1 (en) 2021-02-18
US20210254108A1 (en) 2021-08-19
US11814663B2 (en) 2023-11-14
WO2020030775A1 (en) 2020-02-13
JP2021533764A (ja) 2021-12-09
KR20210043606A (ko) 2021-04-21
IL280749A (en) 2021-04-29
CA3107573A1 (en) 2020-02-13
CN112567044A (zh) 2021-03-26
BR112021002573A2 (pt) 2021-05-11
SG11202101283WA (en) 2021-03-30
EP3833770A1 (en) 2021-06-16
MX2021001619A (es) 2021-05-12

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