AR114547A1 - Microorganismos con producción mejorada de 1,3-propanodiol y ácido butírico - Google Patents
Microorganismos con producción mejorada de 1,3-propanodiol y ácido butíricoInfo
- Publication number
- AR114547A1 AR114547A1 ARP190102267A ARP190102267A AR114547A1 AR 114547 A1 AR114547 A1 AR 114547A1 AR P190102267 A ARP190102267 A AR P190102267A AR P190102267 A ARP190102267 A AR P190102267A AR 114547 A1 AR114547 A1 AR 114547A1
- Authority
- AR
- Argentina
- Prior art keywords
- mutant strain
- clostridium
- propanodiol
- microorganisms
- butyric acid
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/52—Propionic acid; Butyric acids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0067—Oxidoreductases (1.) acting on hydrogen as donor (1.12)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y112/00—Oxidoreductases acting on hydrogen as donor (1.12)
- C12Y112/07—Oxidoreductases acting on hydrogen as donor (1.12) with an iron-sulfur protein as acceptor (1.12.7)
- C12Y112/07002—Ferredoxin hydrogenase (1.12.7.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/0103—Glycerol kinase (2.7.1.30)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente se refiere a una cepa mutante de Clostridium acetobutylicum que comprende actividad atenuada de glicerol quinasa, Además, se refiere a un consorcio de Clostridium que comprende por lo menos dicha cepa mutante y por lo menos otra especie de Clostridium elegida entre C. sporogenes y C. sphenoides. Como esta cepa modificada puede adaptarse para el crecimiento y para la producción de 1,3-propanodiol en un medio de cultivo apropiado con alto contenido de glicerol, la presente además se refiere a un método para la producción de 1,3-propanodiol y ácido butírico, mediante el cultivo de al menos esta cepa mutante en un medio de cultivo apropiado. Reivindicación 1: Una cepa mutante de Clostridium acetobutylicum que expresa los genes dhaB1, dhaB2 y dhaT de C. butyricum y que comprende actividad atenuada de glicerol quinasa.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP18306099 | 2018-08-10 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR114547A1 true AR114547A1 (es) | 2020-09-16 |
Family
ID=63449410
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP190102267A AR114547A1 (es) | 2018-08-10 | 2019-08-09 | Microorganismos con producción mejorada de 1,3-propanodiol y ácido butírico |
Country Status (13)
Country | Link |
---|---|
US (1) | US11814663B2 (es) |
EP (1) | EP3833770A1 (es) |
JP (1) | JP2021533764A (es) |
KR (1) | KR20210043606A (es) |
CN (1) | CN112567044A (es) |
AR (1) | AR114547A1 (es) |
AU (1) | AU2019319045A1 (es) |
BR (1) | BR112021002573A2 (es) |
CA (1) | CA3107573A1 (es) |
IL (1) | IL280749A (es) |
MX (1) | MX2021001619A (es) |
SG (1) | SG11202101283WA (es) |
WO (1) | WO2020030775A1 (es) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT202000031979A1 (it) | 2020-12-22 | 2022-06-22 | Novamont Spa | Processo di purificazione di una miscela comprendente dioli e acetali |
IT202100006419A1 (it) | 2021-03-17 | 2022-09-17 | Novamont Spa | Recupero di dioli da una miscela |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2633939B1 (fr) | 1988-07-06 | 1991-05-31 | Inst Francais Du Petrole | Procede de production d'acide butyrique normal par fermentation a partir d'un substrat sucre en presence d'au moins une souche de l'espece clostridium |
FR2796081B1 (fr) | 1999-07-09 | 2003-09-26 | Agronomique Inst Nat Rech | Procede de preparation du 1,3-propanediol par un micro-organisme recombinant en l'absence de coenzyme b12 ou de l'un de ses precurseurs |
WO2002077183A2 (en) * | 2001-03-21 | 2002-10-03 | Elitra Pharmaceuticals, Inc. | Identification of essential genes in microorganisms |
ES2356526T3 (es) | 2002-10-04 | 2011-04-08 | E.I. Du Pont De Nemours And Company | Procedimiento para la producción biológica de 1,3-propanodiol con elevado rendimiento. |
CN1327001C (zh) | 2005-06-03 | 2007-07-18 | 清华大学 | 利用生物柴油副产物甘油生产1,3-丙二醇的方法 |
JP5203375B2 (ja) | 2006-10-03 | 2013-06-05 | メタボリック エクスプローラー | クロストリジウム菌における染色体取り込みおよびdna配列置換法 |
EP2400027A1 (en) * | 2006-10-31 | 2011-12-28 | Metabolic Explorer | Process for the biological production of 1,3-propanediol from glycerol with high yield |
BRPI0814640A2 (pt) | 2007-07-23 | 2014-10-14 | Dsm Ip Assets Bv | Processo para a preparação de butanol e etanol |
CN101925567B (zh) | 2007-11-30 | 2014-04-23 | 代谢探索者公司 | 用于从发酵液纯化醇的方法 |
MX2011003558A (es) | 2008-10-03 | 2011-05-02 | Metabolic Explorer Sa | Metodo para purificar un alcohol de un caldo de fermentacion usando un evaporador de pelicula descendente, un evaporador de pelicula enjuagada, un evaporador de pelicula delgada o un evaporador de trayectoria corta. |
EP2248904A1 (en) | 2009-05-05 | 2010-11-10 | Metabolic Explorer | Continuous culture for 1,3-propanediol production using high glycerine concentration |
WO2011042434A1 (en) | 2009-10-05 | 2011-04-14 | Metabolic Explorer | 1,3-propanediol production with immobilised cells reactors |
AR083799A1 (es) * | 2010-11-10 | 2013-03-20 | Metabolic Explorer Sa | Microorganismos para la produccion de 1,3-propanodiol usando concentracion de glicerina alta |
GB201116971D0 (en) * | 2011-10-03 | 2011-11-16 | Isis Innovation | Cofactor regeneration system |
KR20140032057A (ko) * | 2012-09-05 | 2014-03-14 | 삼성전자주식회사 | 글리세롤 탈수 반응 산물의 생산 능력이 향상된 재조합 미생물 및 그 용도 |
WO2016042160A1 (en) * | 2014-09-18 | 2016-03-24 | Institut National Des Sciences Appliquees | Clostridium acetobutylicum strains unable to produce hydrogen and useful for the continuous production of chemicals and fuels |
FR3039167B1 (fr) | 2015-07-23 | 2023-11-10 | Afyren | Procede d'extraction de metabolites fermentaires produits par fermentation anaerobie a partir de biomasse fermentescible |
DK3583221T3 (da) * | 2017-02-20 | 2021-09-06 | Metabolic Explorer Sa | Mikrobielt konsortium omfattende clostridia til fremstilling af 1,3-propandiol ud fra glycerol |
-
2019
- 2019-08-09 EP EP19749370.3A patent/EP3833770A1/en active Pending
- 2019-08-09 CN CN201980052879.5A patent/CN112567044A/zh active Pending
- 2019-08-09 AR ARP190102267A patent/AR114547A1/es unknown
- 2019-08-09 CA CA3107573A patent/CA3107573A1/en active Pending
- 2019-08-09 BR BR112021002573-1A patent/BR112021002573A2/pt unknown
- 2019-08-09 US US17/267,347 patent/US11814663B2/en active Active
- 2019-08-09 KR KR1020217006766A patent/KR20210043606A/ko active Search and Examination
- 2019-08-09 SG SG11202101283WA patent/SG11202101283WA/en unknown
- 2019-08-09 WO PCT/EP2019/071392 patent/WO2020030775A1/en unknown
- 2019-08-09 JP JP2021507069A patent/JP2021533764A/ja active Pending
- 2019-08-09 AU AU2019319045A patent/AU2019319045A1/en active Pending
- 2019-08-09 MX MX2021001619A patent/MX2021001619A/es unknown
-
2021
- 2021-02-09 IL IL280749A patent/IL280749A/en unknown
Also Published As
Publication number | Publication date |
---|---|
AU2019319045A1 (en) | 2021-02-18 |
US20210254108A1 (en) | 2021-08-19 |
US11814663B2 (en) | 2023-11-14 |
WO2020030775A1 (en) | 2020-02-13 |
JP2021533764A (ja) | 2021-12-09 |
KR20210043606A (ko) | 2021-04-21 |
IL280749A (en) | 2021-04-29 |
CA3107573A1 (en) | 2020-02-13 |
CN112567044A (zh) | 2021-03-26 |
BR112021002573A2 (pt) | 2021-05-11 |
SG11202101283WA (en) | 2021-03-30 |
EP3833770A1 (en) | 2021-06-16 |
MX2021001619A (es) | 2021-05-12 |
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