WO2020227852A1 - Rh2酶法规模化生产*** - Google Patents

Rh2酶法规模化生产*** Download PDF

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Publication number
WO2020227852A1
WO2020227852A1 PCT/CN2019/086413 CN2019086413W WO2020227852A1 WO 2020227852 A1 WO2020227852 A1 WO 2020227852A1 CN 2019086413 W CN2019086413 W CN 2019086413W WO 2020227852 A1 WO2020227852 A1 WO 2020227852A1
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WIPO (PCT)
Prior art keywords
reaction kettle
production system
liquid separation
solenoid valve
kettle
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PCT/CN2019/086413
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English (en)
French (fr)
Inventor
周志刚
蒋小龙
张琦
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邦泰生物工程(深圳)有限公司
邦泰合盛生物科技（深圳）有限公司
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Application filed by 邦泰生物工程(深圳)有限公司, 邦泰合盛生物科技（深圳）有限公司 filed Critical 邦泰生物工程(深圳)有限公司
Priority to CN201990000212.6U priority Critical patent/CN212077054U/zh
Priority to PCT/CN2019/086413 priority patent/WO2020227852A1/zh
Publication of WO2020227852A1 publication Critical patent/WO2020227852A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control

Definitions

  • the invention relates to the technical field of biocatalysis technology, in particular to an industrial production system that utilizes bioenzyme catalytic technology to produce Rh2 on a large scale.
  • Rh2 is one of the main pharmacologically active ingredients in ginseng saponins-one of ginsenosides. It is a monomer component with significant anti-cancer activity in ginsenosides. It almost represents all the positive effects of ginseng and is regarded as the best Potential natural anti-cancer substance.
  • Rh2 in Panax genus medicinal materials is extremely scarce, and extraction is very difficult
  • companies represented by Bangtai Biotechnology have developed a method for producing Rh2 using biological enzyme catalysis technology, which has the advantages of simple operation, short time, energy saving and environmental protection.
  • biological enzyme catalytic technology Although there have been many reports on the production of Rh2 by biological enzyme catalytic technology, there are no reports on the industrial production system of Rh2 by enzymatic method. It is well-known in the industry that when a new process is applied to large-scale industrial production from small-scale and pilot-scale, there is actually a considerable degree of difficulty in technical transformation. These technical problems often hinder the Rh2 enzymatic preparation process. Used in large-scale industrial production.
  • the purpose of the present invention is to provide a system suitable for large-scale production of Rh2 enzymatically, so that the currently developed Rh2 enzymatic production process can be more conveniently, quickly and directly applied to scale. In the chemical industry production, thus filling the blank of the Rh2 enzymatic industrial production system.
  • the present invention provides a Rh2 enzymatic large-scale production system, which includes a reactor, a cooling water circulation device, a hot steam circulation device, an acid storage tank, an alkali storage tank, and a reactor for detecting The detection device for the degree of reaction, a solid-liquid separation device for solid-liquid separation treatment of materials from the reactor, a washing tank for cleaning the materials after a solid-liquid separation, and a A secondary solid-liquid separation device for solid-liquid separation processing of the material, and a drying device for drying the material after the secondary solid-liquid separation; wherein:
  • the reactor is equipped with a feeding port, a discharging port, a thermometer for measuring the temperature in the kettle, a pH meter for measuring the pH value in the kettle, and a stirring device for stirring the materials in the kettle.
  • a jacket layer for the circulation of cooling water and hot steam;
  • the cooling water circulation device communicates with the jacket layer of the reactor through a pipeline, and is used to provide cooling water for the jacket layer of the reactor to lower the temperature in the reactor;
  • the hot steam circulation device communicates with the jacket layer of the reactor through a pipeline, and is used to provide the jacket layer of the reactor with hot steam for raising the temperature in the reactor;
  • the acid storage tank is connected with the inside of the reaction kettle through a pipeline;
  • the alkali storage tank is connected with the inside of the reaction kettle through a pipeline;
  • the primary solid-liquid separation device is located below the discharge port of the reactor;
  • the washing tank includes a tank body and a stirring device for stirring the materials in the tank body;
  • the secondary solid-liquid separation device is arranged below the discharge port of the washing tank.
  • the inner walls of the reaction kettle, water washing tank, acid storage tank, and alkali storage tank in the above production system are all equipped with acid and alkali resistant corrosion layers, or the corresponding tank body and tank body are made of acid and alkali resistant materials.
  • the detection device in the above-mentioned production system may be any currently known device capable of detecting the progress of the reaction in the reactor, such as a liquid chromatograph.
  • the solid-liquid separation device in the above-mentioned production system can be any device that can separate solids and liquids in materials without destroying the components in the materials, such as centrifuges, plate and frame filter presses, and membranes. Concentration equipment, etc.
  • the drying device in the above-mentioned production system can be any device that can remove moisture from Rh2 products without reducing the content of Rh2 and not destroying the structure of Rh2, such as vacuum dryers, freeze dryers, etc.
  • the acid storage tank and the alkali storage tank are arranged above the reaction vessel, so that the acid liquid and the alkaline liquid can flow into the reaction vessel naturally by gravity, thereby reducing the use of pumps.
  • the above-mentioned production system provided by the present invention further includes a condensing device for cooling the heated and volatilized solvent in the reactor of the reactor, the condensing device is arranged above the reactor and is connected to the reactor through a pipe to form a supply The cooled solvent flows back to the channel in the kettle.
  • the above-mentioned production system provided by the present invention further includes a transfer device for transferring materials, by which the materials can be conveniently put into the reactor, and the materials after a solid-liquid separation can be transferred to the washing tank, or The material after the secondary solid-liquid separation is transferred to the drying device.
  • the transfer device may be a plurality of fixed devices respectively arranged beside each device that needs to transfer materials, or one or more non-fixed devices that can move freely among multiple devices that need to transfer materials. From the perspective of resource and space saving, the transfer device is preferably a non-fixed device, and preferably, the transfer device is a traveling vehicle.
  • the above-mentioned production system provided by the present invention further includes an automatic control system, and the automatic control system includes a temperature automatic control system and a pH automatic control system, wherein:
  • the temperature automatic control system includes a temperature reading device, a temperature control unit, a cooling water solenoid valve and a hot steam solenoid valve; among them, one end of the temperature reading device is electrically connected to the thermometer, the other end is electrically connected to the temperature control unit, and the cooling water solenoid valve is provided On the cooling water circulation device, it is used to control the opening and closing of the cooling water circulation device.
  • the hot steam solenoid valve is installed on the hot steam circulation device to control the opening and closing of the hot steam circulation device.
  • the temperature control unit controls the cooling water solenoid valve separately And hot steam solenoid valve opening and closing;
  • the pH automatic control system includes a pH reading device, a pH control unit, an acid solenoid valve and a lye solenoid valve; among them, one end of the pH reading device is electrically connected to the pH meter, the other end is electrically connected to the pH control unit, and the acid solenoid valve Set on the pipe connecting the acid storage tank and the reaction kettle and close to one end of the reaction kettle, used to control the delivery and stop of the acid liquid into the reaction kettle, and the lye solenoid valve is set on the pipe connecting the alkali storage tank and the reaction kettle The upper part and one end close to the reaction kettle are used to control the delivery and stop of the lye into the reaction kettle.
  • the pH control unit controls the opening and closing of the acid solenoid valve and the lye solenoid valve respectively.
  • the above-mentioned production system provided by the present invention further includes a sewage treatment system for purifying liquid after solid-liquid separation treatment.
  • Rh2 enzymatic large-scale production system provided by the invention has simple structure, perfect function, compact arrangement, space and cost saving, automatic control of temperature and pH value, and labor saving.
  • a separate water washing kettle and corresponding solid-liquid separation device can be set up to realize continuous and uninterrupted reaction of two batches of materials.
  • Figure 1 is a schematic diagram of the structure of the Rh2 enzymatic large-scale production system provided by an embodiment of the present invention
  • this embodiment provides a large-scale Rh2 enzymatic production system.
  • the production system includes a reactor 1, a washing tank 2, an acid storage tank 3, an alkali storage tank 4, a condenser 5, and a hot steam cycle.
  • the inner walls of the acid storage tank 3 and the alkali storage tank 4 are provided with an acid and alkali resistant corrosion layer. among them:
  • the acid storage tank 3 and the alkali storage tank 4 are arranged above the reactor 1 and are respectively communicated with the inside of the reactor 1 through pipes, and an acid solenoid valve 31 is respectively provided at one end of the respective pipes close to the reactor 1 And lye solenoid valve 41;
  • the top of the reactor 1 is provided with a feeding port 11, the bottom is provided with a discharge port 12 and a sampling port 13, and the outer wall is provided with a jacket layer 14.
  • the upper part of the jacket layer 14 is provided with a hot steam inlet 141 and a cooling water outlet 144, respectively.
  • the lower part of the jacket layer 14 is respectively provided with a cooling water inlet 142 and a hot steam outlet 143, and the hot steam inlet 141 and the hot steam outlet 143 are respectively arranged on both sides of the reactor, and the cooling water inlet 142 and the cooling water outlet 144 are respectively arranged On both sides of the reactor;
  • the reactor 1 is also provided with a stirrer 15, a thermometer 16 and a pH meter 17, which extend into the reactor, and a condenser 5 that communicates with the interior of the reactor 1 through a pipe;
  • the hot steam circulation device 6 is respectively connected with the hot steam inlet 141 and the hot steam outlet 143 through pipes to form a circulation of hot steam in the jacket layer 14.
  • the hot steam circulation device 6 is opened and closed by the hot steam solenoid valve 61;
  • the cooling water circulation device 7 is communicated with the cooling water inlet 142 and the cooling water outlet 144, and the condensate inlet 51 and the condensate outlet 52 of the condenser 5 through pipelines, forming a circulation of cooling water in the jacket layer 14 and in the condensing
  • the cooling water circulation device 7 transmits and stops the cooling water circulation in the jacket layer 14 through the cooling water solenoid valve 71 independently provided on the cooling water circulation pipeline;
  • the centrifuge 8 in this embodiment is composed of a primary centrifuge located below the reactor 1 and a secondary centrifuge located below the washing tank 2.
  • the feed inlet of the primary centrifuge is connected to the outlet of the reactor 1 through a pipe.
  • the port 12 is connected, the feed port of the secondary centrifuge is connected to the discharge port 21 of the washing tank 2 through a pipe, and the liquid outlet of the centrifuge 8 is connected to the sewage treatment device 9 through a pipe;
  • the Rh2 enzymatic large-scale production system provided in this embodiment also includes a temperature automatic control system and a pH automatic control system.
  • the temperature automatic control system consists of a temperature reading device (not shown), a temperature control unit (not shown), and a thermal
  • the steam solenoid valve 61 and the cooling water solenoid valve 71 are composed;
  • the pH automatic control system is composed of a pH reading device (not shown), a pH control unit (not shown), an acid solenoid valve 31 and a lye solenoid valve 41.
  • the thermometer 16 measures the temperature in the reactor 1 in real time.
  • the temperature reading device reads the actual temperature value measured by the thermometer 16 and transmits it to the temperature control unit.
  • the temperature control unit compares the actual temperature value with the set temperature value. If the actual temperature value is If the temperature is lower than the set temperature value, the temperature control unit turns on the hot steam circulation device 6 by opening the hot steam solenoid valve 61 to provide circulating hot steam to the jacket layer 14 to heat the reactor 1;
  • the temperature control unit closes the hot steam circulation device 6 by closing the hot steam solenoid valve 61 to stop heating the reactor 1; if the actual temperature value is higher than the set temperature value, the temperature control unit opens The cooling water solenoid valve 71 circulates cooling water to the jacket layer 14 to cool the reactor 1; when the actual temperature value is equal to the set temperature value, the temperature control unit stops the clamping by closing the cooling water solenoid valve 71
  • the jacket 14 conveys cooling water, thereby stopping the continued cooling of the reactor 1.
  • the pH meter 17 measures the pH value in the reactor 1 in real time.
  • the pH reading device reads the actual pH value measured by the pH meter 17 and transmits it to the pH control unit.
  • the pH control unit compares the actual pH value with the set pH value.
  • the pH control unit delivers acid liquid into the reactor 1 by opening the acid liquid solenoid valve 31; when the actual pH value is equal to the set pH value, the pH control unit closes The acid solenoid valve 31 stops sending the acid liquid into the reactor 1; if the actual pH value is lower than the set pH value, the pH control unit sends the lye into the reactor 1 by opening the lye solenoid valve 41; When the pH value is equal to the set pH value, the pH control unit stops delivering lye into the reactor 1 by closing the lye solenoid valve 41;
  • the volatile solvent in the reactor 1 is heated and volatilized, and then enters the condenser 5 through the pipeline, and is cooled and then flows back to the reactor 1 through the pipeline to continue to participate in the reaction. Firstly, it can save the cost of solvent, and secondly, it can prevent the internal cause of the reactor 1. Too much gas is produced and the pressure rises.

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Abstract

本申请公开了一种Rh2酶法规模化生产***，该生产***包括反应釜、冷却水循环装置、热蒸汽循环装置、酸储罐、碱储罐、用于检测反应釜中的反应程度的检测装置、用于对从反应釜出来的物料进行固液分离处理的一次固液分离装置、用于对一次固液分离后的物料进行清洗的水洗罐、用于对从水洗罐出来的物料进行固液分离处理的二次固液分离装置，以及用于对二次固液分离后的物料进行干燥处理的干燥装置。该生产***构成简单、功能完善、排布紧凑、节省空间和成本，对温度和pH值可实现自动化控制，节省人力。

Description

Rh2酶法规模化生产*** 技术领域
本发明涉及生物催化工艺技术领域，特别涉及一种利用生物酶催化技术规模化生产Rh2的工业生产***。
 背景技术
Rh2是人参属药材中起药理活性的主成分—人参皂苷中的一种，是人参皂苷中抗癌活性比较显著的单体成分，几乎代表了人参里的全部正面功效，被视为当今最具潜力的天然抗癌物质。
因为Rh2在人参属药材中的含量极为稀少，提取十分困难，所以以邦泰生物为代表的公司开发了利用生物酶催化技术生产Rh2的方法，该方法具有操作简单、用时短、节能环保的优点。虽然目前已有很多关于生物酶催化技术生产Rh2的工艺报道，但是并未见关于Rh2酶法工业化生产***的报道。为业内所公知的是，将一项新工艺从小试、中试应用于规模化工业大生产上时，其实是存在着相当程度的技术转化难度的，这些技术难题往往成为阻碍Rh2酶法制备工艺应用于规模化工业生产的拦路虎。
 技术问题
鉴于现有技术存在的上述不足，本发明的目的在于提供一种适于Rh2酶法规模化生产的***，以便能够将目前已开发的Rh2酶催化生产工艺更方便更快捷更直接地应用于规模化工业生产中，从而填补Rh2酶法工业化生产***的空白。
 技术解决方案
为实现上述目的，本发明提供了一种Rh2酶法规模化生产***，该生产***包括反应釜、冷却水循环装置、热蒸汽循环装置、酸储罐、碱储罐、用于检测反应釜中的反应程度的检测装置、用于对从反应釜出来的物料进行固液分离处理的一次固液分离装置、用于对一次固液分离后的物料进行清洗的水洗罐、用于对从水洗罐出来的物料进行固液分离处理的二次固液分离装置，以及用于对二次固液分离后的物料进行干燥处理的干燥装置；其中：
反应釜设有投料口、出料口、用于测量釜内温度的温度计、用于测量釜内pH值的pH计、以及用于对釜内物料进行搅拌的搅拌装置， 在反应釜的外壁设有供冷却水和热蒸汽流通的夹套层；
冷却水循环装置通过管道与反应釜的夹套层相连通，用于为反应釜的夹套层提供用于降低釜内温度的冷却水；
热蒸汽循环装置通过管道与反应釜的夹套层相连通，用于为反应釜的夹套层提供用于升高釜内温度的热蒸汽；
酸储罐通过管道与反应釜的釜内相连通；
碱储罐通过管道与反应釜的釜内相连通；
一次固液分离装置设于反应釜的出料口的下方；
水洗罐包括罐体和用于对罐体内的物料进行搅拌的搅拌装置；
二次固液分离装置设于水洗罐的出料口的下方。
上述生产***中的反应釜、水洗罐、酸储罐和碱储罐的内壁均设有耐酸碱性的耐腐层，或者相应的釜体和罐体由耐酸碱的材料制成。
上述生产***中的检测装置可以是目前已知的能够检测出反应釜内的反应进行的程度的任何装置，如液相色谱仪等。
上述生产***中的固液分离装置可以是目前已知的能够将物料中的固体和液体进行分离开同时又不会破坏物料中的成分的任何装置，如离心机、板框压滤机、膜浓缩设备等。
上述生产***中的干燥装置可以是目前已知的能够将Rh2产品中的水分除去同时又不会减少Rh2的含量并且不会破坏Rh2的结构的任何装置，如真空干燥机、冷冻干燥机等。
优选地，本发明提供的上述生产***中，酸储罐和碱储罐设于反应釜的上方，这样设置可使酸液、碱液借助重力作用自然流入反应釜中，从而减少泵的使用。
优选地，本发明提供的上述生产***还包括用于对反应釜的釜内受热挥发的溶剂进行冷却的冷凝装置，该冷凝装置设于反应釜的上方，并通过管道与反应釜相连通形成供冷却后的溶剂回流至釜内的通道。
优选地，本发明提供的上述生产***还包括用于转移物料的转移装置，通过该转移装置可以便捷地将物料投入反应釜内，以及将一次固液分离后的物料转移至水洗罐内，或者将二次固液分离后的物料转移至干燥装置中。该转移装置可以是多个分别设置在每个需要转移物料的装置旁的固定装置，也可以是一个或多个可以在多个需要转移物料的装置间自由移动的非固定装置。从资源及空间节约的角度考虑，转移装置优先选用非固定装置，优选地，转移装置为行车。
为了实现***的自动化、减少人工操作、降低用工成本，优选地，本发明提供的上述生产***还包括自动控制***，该自动控制***包括温度自控***和pH自控***，其中：
温度自控***包括温度读取装置、温度控制单元、冷却水电磁阀和热蒸汽电磁阀；其中，温度读取装置的一端与温度计电连接，另一端与温度控制单元电连接，冷却水电磁阀设于冷却水循环装置上，用于控制冷却水循环装置的开启和关闭，热蒸汽电磁阀设于热蒸汽循环装置上，用于控制热蒸汽循环装置的开启和关闭，温度控制单元分别控制冷却水电磁阀和热蒸汽电磁阀的打开和关闭；
pH自控***包括pH读取装置、pH控制单元、酸液电磁阀和碱液电磁阀；其中，pH读取装置的一端与pH计电连接，另一端与pH控制单元电连接，酸液电磁阀设于酸储罐与反应釜相连通的管道上且靠近反应釜的一端，用于控制酸液向反应釜内的输送和停止，碱液电磁阀设于碱储罐与反应釜相连通的管道上且靠近反应釜的一端，用于控制碱液向反应釜内的输送和停止，pH控制单元分别控制酸液电磁阀和碱液电磁阀的打开和关闭。
为了更好地预防环境污染，实现可持续发展，优选地，本发明提供的上述生产***还包括用于对经固液分离处理后的液体进行净化处理的污水处理***。
 有益效果
本发明提供的Rh2酶法规模化生产***构成简单、功能完善、排布紧凑、节省空间和成本，对温度和pH值可实现自动化控制，节省人力。另外，单独设立水洗釜以及与之对应的固液分离装置，可实现两批物料的连续不间断反应。
 附图说明
图1是本发明实施例提供的Rh2酶法规模化生产***的结构示意图；
图中：
1. 反应釜，11. 投料口，12. 出料口，13. 取样口，14. 夹套层，141. 热蒸汽进口，142. 冷却水进口，143. 热蒸汽出口，144. 冷却水出口，15. 搅拌器，16. 温度计，17. pH计，2. 水洗罐，21. 出料口，22. 搅拌器，3. 酸储罐，31. 酸液电磁阀，4. 碱储罐，41. 碱液电磁阀，5. 冷凝器，51. 冷凝水进口，52. 冷凝水出口，6. 热蒸汽循环装置，61. 热蒸汽电磁阀，7. 冷却水循环装置，71. 冷却水电磁阀，8. 离心机，9. 污水处理装置。
 本发明的实施方式
下面结合附图和具体实施例对本发明做进一步的详细说明，以下实施例是对本发明的解释，本发明并不局限于以下实施例。
如图1所示，本实施例提供了一种Rh2酶法规模化生产***，该生产***包括反应釜1、水洗罐2、酸储罐3、碱储罐4、冷凝器5、热蒸汽循环装置6、冷却水循环装置7、离心机8、污水处理装置9、行车（未图示）、液相色谱仪（未图示）和真空干燥机（未图示），反应釜1、水洗罐2、酸储罐3和碱储罐4的内壁均设有耐酸碱性的耐腐层。其中：
酸储罐3和碱储罐4设于反应釜1的上方，分别通过管道与反应釜1的内部相连通，并且，在各自的管道上靠近反应釜1的一端分别设置有酸液电磁阀31和碱液电磁阀41；
反应釜1的顶部设有投料口11，底部设有出料口12和取样口13，外壁设有夹套层14，夹套层14的上部分别设有热蒸汽进口141和冷却水出口144，夹套层14的下部分别设有冷却水进口142和热蒸汽出口143，并且，热蒸汽进口141和热蒸汽出口143分别设置在反应釜的两侧，冷却水进口142和冷却水出口144分别设置在反应釜的两侧；反应釜1上还设有伸入釜内的搅拌器15、温度计16和pH计17，以及通过管道与反应釜1的内部相连通的冷凝器5；
热蒸汽循环装置6通过管道分别与热蒸汽进口141和热蒸汽出口143相连通，形成热蒸汽在夹套层14的循环流通，热蒸汽循环装置6通过热蒸汽电磁阀61开启和关闭；
冷却水循环装置7通过管道分别与冷却水进口142和冷却水出口144以及冷凝器5上的冷凝水进口51和冷凝水出口52相连通，形成冷却水在夹套层14的循环流通，以及在冷凝器5内的循环流通，冷却水循环装置7通过独立设于冷却水循环管道上的冷却水电磁阀71输送和停止夹套层14内的冷却水循环；
本实施例中的离心机8由分别设于反应釜1下方的一次离心机和设于水洗罐2下方的二次离心机组成，一次离心机的进料口通过管道与反应釜1的出料口12相连通，二次离心机的进料口通过管道与水洗罐2的出料口21相连通，离心机8的液体出口通过管道与污水处理装置9相连通；
另外，本实施例提供的Rh2酶法规模化生产***还包括温度自控***和pH自控***，其中，温度自控***由温度读取装置（未图示）、温度控制单元（未图示）、热蒸汽电磁阀61和冷却水电磁阀71 组成；pH自控***由pH读取装置（未图示）、pH控制单元（未图示）、酸液电磁阀31和碱液电磁阀41组成。
应用本实施例提供的上述生产***时，先将所需物料通过投料口11投入反应釜1内，为温度控制单元设定好所需温度，为pH控制单元设定好所需pH值，开启搅拌器15、温度读取装置、pH读取装置和冷却水循环装置；
温度计16实时测量反应釜1内的温度，温度读取装置读取温度计16测量的实际温度数值并传输给温度控制单元，温度控制单元将实际温度数值与设定温度数值进行比较，如果实际温度数值低于设定温度数值，则温度控制单元通过打开热蒸汽电磁阀61而开启热蒸汽循环装置6，为夹套层14提供循环热蒸汽，以对反应釜1进行加热；待实际温度数值等于设定温度数值时，则温度控制单元通过关闭热蒸汽电磁阀61而关闭热蒸汽循环装置6，从而停止对反应釜1继续加热；如果实际温度数值高于设定温度数值，则温度控制单元通过打开冷却水电磁阀71而向夹套层14循环输送冷却水，以对反应釜1进行降温；待实际温度数值等于设定温度数值时，则温度控制单元通过关闭冷却水电磁阀71而停止向夹套层14输送冷却水，从而停止对反应釜1继续降温。
同时，pH计17实时测量反应釜1内的pH值，pH读取装置读取pH计17测量的实际pH数值并传输给pH控制单元，pH控制单元将实际pH数值与设定pH数值进行比较，如果实际pH数值高于设定pH数值，则pH控制单元通过打开酸液电磁阀31而向反应釜1内输送酸液；待实际pH数值等于设定pH数值时，则pH控制单元通过关闭酸液电磁阀31而停止向反应釜1内输送酸液；如果实际pH数值低于设定pH数值，则pH控制单元通过打开碱液电磁阀41而向反应釜1内输送碱液；待实际pH数值等于设定pH数值时，则pH控制单元通过关闭碱液电磁阀41而停止向反应釜1内输送碱液；
同时，反应釜1内的易挥发溶剂受热挥发后通过管道进入冷凝器5内被冷却后通过管道回流至反应釜1内继续参与反应，一来可以节省溶剂成本，二来可以防止反应釜1内因过多气体产生而压力升高。
同时，通过取样口13实时取样并通过液相色谱仪检测反应釜内的反应进行的程度，待反应完全后，通过出料口12将物料排入离心机8中进行固液分离处理，分离出来的液体通过管道流入污水处理装置9中进行净化处理，待离心结束后，通过行车将分离的固体物料转移至水洗罐2内，加入水后开启搅拌器22进行搅拌，搅拌均匀后通过出料口21排入离心机8中进行固液分离处理，分离出来的液体通过管道流入污水处理装置9中进行净化处理，待离心结束后，通过行车将分离的固体物料转移至真空干燥机内进行干燥处理，即得Rh2产品。

Claims (9)

  1. Rh2酶法规模化生产***，其特征在于，所述生产***包括：
    反应釜，所述反应釜设有投料口、出料口、用于测量釜内温度的温度计、用于测量釜内pH值的pH计，以及用于对釜内物料进行搅拌的搅拌装置，所述反应釜的外壁设有供冷却水和热蒸汽流通的夹套层；
    冷却水循环装置，所述冷却水循环装置通过管道与所述反应釜的夹套层相连通，用于为所述反应釜的夹套层提供用于降低釜内温度的冷却水；
    热蒸汽循环装置，所述热蒸汽循环装置通过管道与所述反应釜的夹套层相连通，用于为所述反应釜的夹套层提供用于升高釜内温度的热蒸汽；
    酸储罐，所述酸储罐通过管道与所述反应釜的釜内相连通；
    碱储罐，所述碱储罐通过管道与所述反应釜的釜内相连通；
    检测装置，用于检测所述反应釜中的反应程度；
    一次固液分离装置，所述一次固液分离装置设于所述反应釜的出料口的下方，用于对从所述反应釜出来的物料进行固液分离处理；
    水洗罐，包括罐体和用于对罐体内的物料进行搅拌的搅拌装置，所述水洗罐用于对一次固液分离后的物料进行清洗；
    二次固液分离装置，所述二次固液分离装置设于所述水洗罐的出料口的下方，用于对从所述水洗罐出来的物料进行固液分离处理；
    干燥装置，用于对二次固液分离后的物料进行干燥处理。
  2. 根据权利要求1所述的Rh2酶法规模化生产***，其特征在于：所述酸储罐和所述碱储罐设于所述反应釜的上方。
  3. 根据权利要求1所述的Rh2酶法规模化生产***，其特征在于：所述生产***还包括用于对所述反应釜的釜内受热挥发的溶剂进行冷却的冷凝装置，所述冷凝装置设于所述反应釜的上方，并通过管道与所述反应釜相连通形成供冷却后的溶剂回流至釜内的通道。
  4. 根据权利要求1所述的Rh2酶法规模化生产***，其特征在于：所述生产***还包括用于转移物料的转移装置。
  5. 根据权利要求4所述的Rh2酶法规模化生产***，其特征在于：所述转移装置为行车。
  6. 根据权利要求1所述的Rh2酶法规模化生产***，其特征在于：所述生产***还包括自动控制***，所述自动控制***包括用于自动控制所述反应釜的釜内温度升降的温度自控***和用于自动控制所述反应釜的釜内pH值增减的pH自控***。
  7. 根据权利要求6所述的Rh2酶法规模化生产***，其特征在于：所述温度自控***包括温度读取装置、温度控制单元、用于控制所述冷却水循环装置开启和关闭的冷却水电磁阀，以及用于控制所述热蒸汽循环装置开启和关闭的热蒸汽电磁阀；所述温度读取装置的一端与所述温度计电连接，另一端与所述温度控制单元电连接，所述温度控制单元分别控制所述冷却水电磁阀和所述热蒸汽电磁阀的打开和关闭。
  8. 根据权利要求6所述的Rh2酶法规模化生产***，其特征在于：所述pH自控***包括pH读取装置、pH控制单元、用于控制所述酸储罐中的酸液向所述反应釜内输送和停止的酸液电磁阀，以及用于控制所述碱储罐中的碱液向所述反应釜内输送和停止的碱液电磁阀；所述pH读取装置的一端与所述pH计电连接，另一端与所述pH控制单元电连接，所述酸液电磁阀设于所述酸储罐与所述反应釜相连通的管道上且靠近所述反应釜的一端，所述碱液电磁阀设于所述碱储罐与所述反应釜相连通的管道上且靠近所述反应釜的一端，所述pH控制单元分别控制所述酸液电磁阀和所述碱液电磁阀的打开和关闭。
  9. 根据权利要求1至8任一项所述的Rh2酶法规模化生产***，其特征在于：所述生产***还包括用于对经固液分离处理后的液体进行净化处理的污水处理***。
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