WO2020004655A1 - 生体試料の加温方法、生体試料の加温容器、及び生体試料を加温するためのキット - Google Patents
生体試料の加温方法、生体試料の加温容器、及び生体試料を加温するためのキット Download PDFInfo
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- WO2020004655A1 WO2020004655A1 PCT/JP2019/025962 JP2019025962W WO2020004655A1 WO 2020004655 A1 WO2020004655 A1 WO 2020004655A1 JP 2019025962 W JP2019025962 W JP 2019025962W WO 2020004655 A1 WO2020004655 A1 WO 2020004655A1
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- biological sample
- heat medium
- storage container
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- heating
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/20—Heating; Cooling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/22—Means for packing or storing viable microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
Definitions
- the present invention relates to a biological sample heating method, a biological sample heating container, and a kit for heating a biological sample.
- Patent Document 1 describes a frozen cell thawing apparatus that heats and thaws cryopreserved cells and tissues with a heater that heats the cells and tissues to a temperature higher than their melting points.
- ⁇ Cell freezing technology is indispensable in the production of regenerative medicine cell products and cell research. By freezing and thawing cells stably without changing the properties of the cells, it is possible to improve the productivity of cell products for regenerative medicine, and has high reliability with little variation in cell research. Data can be obtained.
- a typical conventional thawing method there is known a method of thawing frozen cells using a water bath.
- a water bath requires a large amount of water, and has a problem that the volume and weight of the device are large.
- various bacteria grow in the water serving as a heat medium. It's easy to do.
- a conventional frozen cell thawing apparatus that uses a heater as a heat source and thaws through a solid-phase heat medium, that is, a heat block thermostat (heat block) does not require a large amount of water, and the apparatus is small.
- a heat block thermostat heat block
- the heat block has a lower heat transfer efficiency than the water bath, and the thawing time is longer, so that the risk of damage to the cells due to the concentration gradient or the temperature gradient during thawing increases. .
- One embodiment of the present invention has been made to solve the above problems, and an object thereof is to realize a simple and safe heating method in which damage to a biological sample is suppressed.
- a heating method is a method for heating a biological sample, wherein the biological medium containing the biological sample is placed in a heat medium containing container containing a heat medium.
- a heating container is a heating container for a biological sample, and a heating medium storage container that stores a biological sample storage container that stores a biological sample and that stores a heat medium therein. And a position defining unit provided on the heat medium container for defining the position of the biological sample container.
- a kit for heating a biological sample includes a heat medium storage container for storing a heat medium and a biological sample storage container, and the heat medium storage container includes the biological sample storage container. It has a position defining part for defining the position of the container, and the biological sample storage container is for storing a biological sample.
- damage to a biological sample can be suppressed, and the biological sample can be easily and safely heated.
- FIG. 2 is a schematic diagram illustrating a heating container used in the heating method according to one embodiment of the present invention. It is a mimetic diagram showing the heating container used with the heating method concerning other modes of the present invention. It is a schematic diagram which shows the heating container which concerns on another aspect. It is a schematic diagram which shows the heating container which concerns on another aspect. It is a schematic diagram which shows the heating container which concerns on another aspect. It is a schematic diagram which shows the heating container which concerns on another aspect. It is a schematic diagram which shows the heating container which concerns on another aspect. It is a schematic diagram which shows the heating container which concerns on another aspect.
- FIG. 2 is a schematic view illustrating a heating kit according to one embodiment of the present invention. It is a figure showing an example of inversion mixing.
- FIG. 3 is a diagram illustrating an example of dimensions of a resin film. It is a figure showing an example of the state where a heating container was grasped by a human hand. It is the figure which expanded a part of FIG. It is the figure which looked at an example of the heating container from the top. It is a figure showing an example of the state where a living body sample container was stored in a heat carrier container. It is a graph which shows a decompression result. It is a graph which shows a decompression result. It is a graph which shows a decompression result. It is a graph which shows a decompression result. It is a graph which shows the proliferation ability of the thawed cell.
- the heating method is a method for heating a biological sample, and a heating medium containing a heat medium, and a housing step of housing a biological sample container containing the biological sample.
- a heating method is a method for heating a biological sample, wherein a housing step of housing a biological sample housing container housing a biological sample in a heat medium housing container housing a heat medium. And after the housing step, a closing step of closing an inlet of the heat medium of the heat medium housing container so that the heat medium does not leak out of the heat medium housing container; and an opening of the heat medium is closed. Moving the heat medium container together.
- the “biological sample” means a sample derived from a living body, and is preferably at least one selected from the group consisting of cells, cell masses, tissues, and tissue fragments.
- the cells as biological samples include various useful cells, for example, mesenchymal stem cells (MSCs) derived from various tissues, iPS cells and cell lines derived therefrom, ES cells and cell lines derived therefrom, hematopoietic stem cells And other stem cells such as neural stem cells, cancer cells, vascular progenitor cells, vascular cells, myoblasts, umbilical cord-derived cells, chondrocytes, osteoblasts, intervertebral disc cells, genetically modified cells, and the like.
- MSCs mesenchymal stem cells
- Tissue as a biological sample includes various useful tissues, for example, bone marrow fluid, cord blood, cord tissue, various bone marrow-derived cell fractions, adipose tissue pieces, sperm, eggs, cadaver-derived allogeneic or autologous cartilage tissue, Bone tissue and the like.
- the tissue as a biological sample includes an ES cell-derived tissue, an iPS cell-derived tissue, and a tissue for transplantation produced by tissue engineering containing various cells.
- the biological sample to be heated may be a frozen biological sample or an unfrozen biological sample. That is, the heating method according to one embodiment of the present invention can also thaw a frozen sample, and, for example, when recovering cells and tissues stored by a non-freezing cryopreservation method to room temperature or body temperature. Can also be used.
- the “frozen biological sample” is also referred to as a “frozen sample”.
- “Frozen sample” means a sample that has been cryopreserved.
- the frozen sample is preferably a sample stored in a very low temperature environment such as ⁇ 250 ° C. or higher and ⁇ 60 ° C. or lower for a certain period of time such as several hours to several years or more.
- the method for freezing the biological sample to be heated in the heating method according to one embodiment of the present invention is not particularly limited, and a conventionally known freezing method may be used.
- the biological sample may be a biological sample frozen while maintaining a three-dimensional structure by a method such as using a scaffold, or a biological sample frozen and stored using a cryopreservation solution.
- the solution for cryopreservation may contain a fatty acid, a phospholipid, a surfactant, and the like, and one or more of these may be used.
- the “biological sample that has not been frozen” includes a biological sample stored for a certain period of time in an ultra-low temperature environment.
- a biological sample include cells and tissues preserved by the above-described non-freezing cryopreservation method, cadaver-derived allogeneic or autologous cartilage tissues preserved by the non-freezing cryopreservation method, and cells for various transplantation. No.
- the non-freezing low-temperature preservation method is a preservation method in a low-temperature and non-freezing environment such as refrigeration (5 ° C. to 10 ° C.) or chilled (0 ° C. to 5 ° C.). It means a method of preserving a tissue, a cell, a cell mass, or the like, preferably in a state of being immersed in an isotonic solution containing the same.
- heating means increasing the temperature by applying heat to a biological sample.
- the biological sample is a frozen sample
- thawed means that at least a part of the solid phase of the frozen sample is converted to a liquid phase. It means completely melting and returning to the liquid phase.
- cryopreservation and non-frozen low-temperature preservation may be simply referred to as “low-temperature preservation”.
- the biological sample accommodation container accommodating the biological sample is accommodated in the heat medium accommodation container accommodating the heat medium.
- “contains B in container A” means (case 1) of container A having a space inside container A, putting B in the space, or (case 2) of container A This means that B in contact with the outer surface is wrapped by the container A. (Case 2) further means that the container A is made of a deformable material.
- the accommodation step will be described in detail by taking (case 1) as an example.
- the heat medium storage container stores a heat medium therein, and has an opening serving as an inlet for the heat medium. An example of the heat medium storage container will be described with reference to FIG. FIG.
- FIG. 1 is a schematic diagram illustrating a heating vessel used in the heating method according to one embodiment of the present invention.
- the heating container 1 has a heat medium storage container 2.
- the heat medium container 2 is provided with an opening 3 for injecting the heat medium 4.
- the heat medium storage container 2 stores a biological sample storage container 5 that stores a biological sample.
- FIG. 1 shows, as an example, a state in which the opening 3 is closed by a lid 6 in a closing step described later.
- the heat medium storage container may be any container that can store the heat medium. As will be described later, the heat medium used in the present invention does not reach a high temperature, so that the heat medium container does not need to be heat resistant. Specifically, a container made of a synthetic resin such as polyethylene, polypropylene, polystyrene, or polyethylene terephthalate is more preferable as the heat medium storage container.
- the heat medium storage container is preferably one that can be easily discarded after use, thereby preventing cross contamination.
- the heat medium storage container is preferably a cylindrical structure having one end closed and the other end open so that it can be easily grasped by human hands, and a cross section perpendicular to the longitudinal direction of the cylindrical structure. Is more preferably 5 mm or more and 200 mm or less. Further, it is preferable that the heat medium storage container be portable by human hands.
- a commercially available centrifuge tube can be suitably used as the heat medium storage container.
- the heat medium container may further include a position defining unit for defining the position of the biological sample container inside.
- a position defining unit for defining the position of the biological sample container inside.
- FIG. 2 is a schematic diagram showing a heating vessel 10 used in a heating method according to another embodiment of the present invention. As shown in FIG. 2, the heating container 10 is different from the heating container 1 shown in FIG. 1 in that the heating container 10 includes a resin film 11 functioning as a position defining unit.
- the resin film 11 has a bag shape so that the biological sample container 5 can be accommodated therein.
- the resin film 11 is preferably an elastic film, and examples thereof include nitrile rubber, polyurethane, and natural rubber.
- the resin film 11 is provided so as to cover the opening 3, hook a part of the entrance of the bag on the opening 3, and adhere to the heat medium container 2.
- the position where the biological sample storage container 5 is stored is isolated by the resin film 11 so that the heat medium 4 and the biological sample storage container 5 are not physically in contact with each other.
- FIG. 2 shows an example in which the opening 3 is closed by the lid 6 in the closing step.
- the biological sample storage container since the movement of the biological sample storage container in the heat medium storage container is restricted by the position of the heat medium storage container provided with the position defining portion, the biological sample storage container largely moves in the exercise process described below. This can prevent the container from being damaged by colliding with the lid or the like.
- the position defining section particularly has a function of isolating the physical contact between the heat medium 4 and the biological sample storage container 5, the heat medium and the biological sample storage container do not come into direct contact in the heat medium storage container. In addition, the risk that the heat medium flows into the biological sample container and the biological sample is contaminated can be reduced.
- the heat medium is stored in the heat medium storage container through the opening.
- the heat medium may be any fluid that can exchange heat with the biological sample in the biological sample container, and any fluid having a heat capacity that can maintain a predetermined temperature range for a predetermined time. Therefore, a fluid having a high specific heat is preferable because it exhibits a high heat capacity even when the amount of the heat medium is small.
- the heat medium is preferably at least one selected from the group consisting of water, an isotonic solution, and water in which an antibacterial agent is dissolved.
- the amount of the heat medium to be accommodated in the heat medium storage container is set in consideration of conditions such as the amount of the biological sample and the temperature of the heat medium, so that the heat medium has a sufficient heat capacity to heat the biological sample. Good.
- the biological sample storage container is stored on the outer surface thereof (case 2).
- the heat medium storage container wraps the biological sample storage container. Therefore, the heat medium storage container of (Case 2) is a container made of a flexible material.
- Order estimation based on how much temperature change occurs in the heat medium due to heat exchange between the biological sample and the heat medium, order estimation of the amount of the heat medium and the capacity of the heat medium storage container is performed as follows. Can be. Based on the result of such order estimation, the type, amount and temperature of the heat medium, the volume of the heat medium container, and the like may be designed.
- the biological sample and the heat medium in the biological sample container have the same specific heat, specific gravity, melting point, etc. as water or ice.
- the weight change of the heat medium is calculated when the weight of the biological sample is 1 g, the weight of the heat medium is 40 g, and the initial temperature of the biological sample is ⁇ 80 ° C. and the initial temperature of the heat medium is 24 ° C. Examples will be described, but the present invention is not limited to these values.
- the heat medium does not exchange heat with anything other than the biological sample, and it is assumed that all heat of the heat medium is used to change the state and temperature of the biological sample. Estimate order by three steps of calculation.
- ⁇ means multiplication
- “/” means division
- ⁇ ” means power.
- a numerical value representing a physical quantity Q of a certain size in a certain unit u is expressed as Q [u].
- the temperature T of the heat medium after the process i is approximately 23 ° C.
- the initial temperature of the heat medium is 24 ° C., and the heat medium does not reach the freezing point at such a temperature change, and the temperature change is very small. Therefore, heat exchange with the external environment can occur, and thus can be ignored in actual use. Temperature change.
- the temperature of the heat medium that has gone through the processes i and ii is approximately 21 ° C. With such a temperature change, since the change in heat is very small, it is a temperature change that can be ignored in actual use where heat exchange with the external environment can occur.
- ⁇ Iii Process of changing biological sample from 0 ° C. liquid to equilibrium temperature liquid> According to the law of conservation of heat, the equilibrium temperature T ⁇ when a fluid having a temperature T, a mass M, and a specific heat C is brought into contact with a fluid having a temperature T ′, a mass M ′, and a specific heat C ′ undergoes a phase change.
- the temperature T of the heating medium changes from 24 ° C. to 20.5 ° C. due to the melting in the steps i to iii, so that the temperature drops 3.5 ° C. as a whole.
- heat transfer between the heat medium and the external environment ignored in the order estimation exists, so that the temperature drop of the heat medium as described above is negligible in actual use.
- various thermal conditions such as the type, amount, and temperature of the heat medium are set so as to be substantially the same as the above-described temperature change.
- the amount of the heat medium is such that forced convection occurs in the exercise process described below, and the heat medium and the biological sample can be brought into sufficient thermal contact with each other. Any quantity characterized by a negligible temperature change may be used.
- the biological sample may be stored in the biological sample storage container for each unit to be heated, or a plurality of units may be collectively stored in the biological sample storage container.
- a biological sample stored in the biological sample storage container for each unit to be heated it is only necessary to take out the biological sample storage container from the storage location of the biological sample storage container.
- the biological sample storage container is taken out from the storage location, and then transferred to another biological sample storage container for each unit for use.
- the biological sample container may contain a sample used for freezing or a frozen sample, or a sample stored by a non-freezing and low-temperature preservation method, and therefore may be any container that can withstand freezing and low temperatures.
- the biological sample container is preferably capable of withstanding ⁇ 80 ° C., and more preferably capable of withstanding ⁇ 250 ° C.
- a container made of a synthetic resin such as polyethylene, polypropylene, polystyrene, or polyethylene terephthalate is more preferable as the biological sample container.
- the biological sample container is sealed in order to prevent the biological sample in the biological sample container from being contaminated and to prevent the biological sample from flowing out of the biological sample container and contaminating the worker or the workplace. Is preferred. Since the biological sample storage container is stored in the heat medium storage container that stores the heat medium, it is particularly preferable that the container be hermetically sealed so that the heat medium does not flow.
- the biological sample container is accommodated in the heat medium container so that the contained biological sample and the heat medium can exchange heat via the biological sample container.
- the biological sample container is placed inside the heat medium container so that the outer wall of the biological sample container and the heat medium physically contact at least in part. May be accommodated. That is, it is preferable to store the biological sample container in the heat medium container so that at least a part of the biological sample container is immersed in the heat medium.
- the heat medium container which is a cylindrical body
- the heat is applied so that the entire biological sample in the biological sample container is located below the liquid surface of the heat medium in the vertical direction.
- the biological sample container may be accommodated in the medium container.
- the heat medium was convected in the exercise process described below, even if the outer wall of the biological sample storage container was not in physical contact with the heat medium. At this time, if the outer wall of the biological sample storage container and the heat medium physically contact each other, more efficient heat exchange between the biological sample and the heat medium becomes possible.
- the biological sample storage container may be stored in the heat medium storage container so that the heat medium and the biological sample can exchange heat via the position determining portion.
- heat exchange between the heat medium and the biological sample is performed, but since the heat medium does not directly contact the biological sample container, there is a risk that the heat medium flows into the biological sample container and the biological sample is contaminated. Can be reduced.
- the inlet of the heat medium of the heat medium storage container is closed so that the heat medium does not leak out of the heat medium storage container.
- it can be closed by covering the heat medium inlet of the heat medium storage container with a lid.
- the position defining unit or the like does not have a function of sealing outflow of the heat medium from the heat medium storage container (for example, see FIG. 5 described later).
- the position defining portion is not provided (see, for example, FIG. 1), the heat medium and the biological sample container are ejected from the heat medium inlet even when the heat medium container is moved in the movement step described later. Therefore, contamination of the workers and the workplace can be prevented.
- the closing step is not limited to the order in which it is performed as long as the purpose (the heat medium does not leak out of the heat medium storage container) is achieved.
- the closing step is performed after the storing step.
- An accommodation step may be performed.
- the closing step is not limited to the member that closes the entrance as long as the purpose is achieved.
- the resin film 11 in FIG. 2 functions as a position fixing portion for fixing the position of the biological sample storage container 5
- the resin film 11 also functions to prevent the heat medium 4 from leaking out of the heat medium storage container 2.
- the member for preventing the heat medium from leaking out of the heat medium storage container from the inlet is provided before the movement step (at any time after the heat medium 4 is stored in the heat medium storage container 2).
- Providing a mouth corresponds to a closing step.
- the lid 6 in FIG. 2 covers the opening 3, and prevents the biological sample storage container 5 from jumping out of the heat medium storage container 2 in the exercise process described later. Since the heat medium in the heat medium storage container does not leak from the pocket-shaped position defining portion 3 to the space where the biological sample storage container is arranged, the heat medium storage container 2 No heat medium leaks from inside to outside.
- the exercising step it is preferable to move the heat medium accommodating container with the heat medium inlet closed.
- the heat medium container is moved together with the biological sample container so that the heat medium convects in the heat medium container and the heat medium circulates in the heat medium container.
- the exercise step is not limited to the means of implementation as long as the purpose of exercising the heat medium in the heat medium container 2 whose inlet is closed is achieved.
- a nozzle that supplies a fluid into the heat medium storage container 2 may partially close the inlet.
- a fan may be provided in the heat medium storage container 2 in which the inlet is closed. The nozzle and the fan (means for circulating the heat medium) both cause the heat medium in the heat medium container 2 to circulate. If the nozzle or the fan is provided, it is not necessary to move the heat medium storage container 2 in the exercise step.
- the exercising step may be performed using a device that applies vibration to the heat medium storage container, but it is preferable that the heat medium storage container be moved by grasping and shaking the heat medium storage container by hand. Since the heat medium storage container is shaken while grasping the heat medium storage container by hand, it is not necessary to use a device that applies vibration, which is simpler. In particular, in the case where cells to be heated are heated in an operating room where transplantation surgery is performed, it is difficult to bring in the above-described device, and it is more preferable to shake the cells by hand. .
- a device that applies vibration to the entire heat medium accommodating container a device known in the art (a tube rotator, a shaker, and the like) is used.
- a mechanism for circulating the heat medium without directly applying vibration to the heat medium storage container for example, a nozzle or a fluid for ejecting a fluid into the heating container is circulated in the heating container, It is conceivable to provide a circulating means such as a fan or the like for increasing the efficiency of heat exchange between the material storage container and the heat medium.
- the exercise step is performed in a state where the heat medium is at least 20 ° C and at most 40 ° C.
- the biological sample can be heated in a shorter time while suppressing damage to the biological sample.
- the heating medium is in a state of 20 ° C. or more and 40 ° C. or less, the heating temperature is almost the same as the heating at 37 ° C. in a conventional thawing method using a water bath.
- the temperature of the heat medium means the temperature before heat exchange with the biological sample.
- the exercise step is performed in a state where the heat medium is at least 20 ° C. and no more than 27 ° C. Accordingly, since the temperature of the heat medium is substantially equal to the room temperature, there is no need to heat the heat medium, and a heat source as a heating element for heating the heat medium is unnecessary. In addition, even when the heat medium is lower than the above temperature range, the temperature can be heated to the above temperature range by the body temperature only by grasping the heat medium storage container by hand.
- the exercise step it is preferable to shake the heat medium storage container for 10 seconds or more and 600 seconds or less.
- the time for shaking the heat medium storage container is within the above range, the heat medium efficiently convects in the heat medium storage container, and heat exchange between the heat medium and the biological sample can be performed more efficiently.
- the heat medium container In the exercise step, it is preferable to shake the heat medium container at 30 rpm or more and 120 rpm or less.
- the cycle of shaking the heat medium storage container is within the above range, the heat medium efficiently convects in the heat medium storage container, and heat exchange between the heat medium and the biological sample can be performed more efficiently.
- the heat medium storage container has a cylindrical structure in which one end is closed and the other end is open
- one end in the longitudinal direction of the cylindrical structure is fixed, and the swing angle of the other end is increased. It is preferable to shake the heat medium storage container so that it becomes 90 degrees or more. Shaking the heat medium storage container in this manner is referred to as overturn mixing in this specification.
- the swing angle of the overturning mixing is more preferably 120 degrees or more, and even more preferably 150 degrees or more. Further, it is preferable that the heat medium storage container is overturned and mixed so that the vertical positions of both ends in the length direction of the cylindrical body are switched at least once in the vertical direction. That is, when one end of the heat medium storage container is gripped by a human hand so that the length direction of the heat medium storage container is vertical, and the other end is shaken in the horizontal direction, the heat medium storage container is overturned and mixed. It is preferable to increase the swing width so that the other end is located above the one end because the heat medium convects more.
- the heat medium is circulated to such an extent that the forced convection of the heat medium occurs sufficiently efficiently. Therefore, surprisingly, even when the temperature of the heat medium is low, such as 22 ° C., the heat medium can be added in a short time. Warm is possible. Since this temperature is lower than the standard temperature of 37 ° C. which is a standard temperature in a conventional general thawing method using a water bath or the like, thermal damage to a biological sample is significantly less than in the conventional method. In addition, since 22 ° C. is a general room temperature, a heat source for warming the heat medium is not required for inverting and mixing the heat medium container.
- the conventional method using a water bath requires a large amount of water of the order of 10 L as a heat medium, is not easy to carry, is troublesome for daily cleaning, and when neglecting daily cleaning, germs can propagate and become improper. There were various problems of hygiene.
- the heating method of one embodiment of the present invention since only a very small amount of heat medium, for example, 50 mL or less, is required, it is easy to carry, and is easily discarded after use and daily cleaning is performed. Not required.
- the conventional method of thawing a biological sample by heating with a heater does not require a large amount of water and the device is small, but the biological sample is easily damaged because the heater temporarily becomes hot. There was a problem. In addition, there was a variation in performance between heater devices used, and reproducibility of heating could not be ensured in some cases.
- the heating method according to one embodiment of the present invention since the heating medium can be heated in a short time even at a low temperature close to room temperature, heat damage to the biological sample is reduced. Further, a heat source for heating the heat medium is not required. Furthermore, since the heating is carried out by a very simple operation of holding and shaking the heat medium storage container containing the heat medium with the hand of a person, variation does not easily occur with each heating.
- a heating container is a heating container for a biological sample, which stores a biological sample storage container in which a biological sample is stored, and a heat medium in which a heat medium is stored.
- the container includes a storage container, and a position defining unit provided in the heat medium storage container for defining a position of the biological sample storage container.
- a heating container is a heating container for a biological sample, a biological sample storage container in which a biological sample is stored, and a heat medium storage container in which a heat medium is stored.
- a position defining unit for defining a position of the biological sample container provided in the heat medium container is, for example, a biological sample, preferably a heating container for heating a frozen or refrigerated biological sample in a state of being stored in the biological sample storage container,
- the heating container 1 includes a heat medium storage container 2.
- the heating container 1 is for storing the biological sample storage container 5 in the heat medium storage container 2 and heating the biological sample stored in the biological sample storage container 5.
- the heat medium storage container 2 stores the heat medium, and further stores the biological sample storage container 5 and is closed by the lid 6.
- the heat medium 4 is housed in the heat medium housing container 2.
- the biological sample storage container 5 is stored in the heat medium storage container 2 through the opening 3, and heat exchange between the biological sample in the biological sample storage container 5 and the heat medium 4 is possible via the biological sample storage container 5. .
- the opening 3 is closed by a lid 6 so that the heat medium 4 and the biological sample container 5 do not come out of the heat medium container 2 of the heating container 1.
- the heating container further includes a resin film 11 functioning as a position defining unit, as in the heating container 10 shown in FIG.
- the resin film 11 has a bag shape (pocket shape) so that the biological sample storage container 5 can be stored therein.
- the resin film 11 is provided so as to cover the opening 3, hook a part of the entrance of the bag on the opening 3, and adhere to the heat medium container 2. Therefore, while the resin film 11 in FIG. 2 functions as a position fixing portion for fixing the position of the biological sample storage container 5, the resin film 11 also functions to prevent the heat medium 4 from leaking out of the heat medium storage container 2. .
- a parafilm or the like may be wound around a portion of the resin film 11 hooked on the opening 3.
- the parafilm does not have to be excessively wound, but if it is desired to fix more firmly, the parafilm may be fixed by various kinds of appropriate bonding and winding fixing known to those skilled in the art.
- the heat medium storage container 2 may be any as long as it can store the heat medium inserted from the opening 3. Since the heat medium used in the present invention does not reach a high temperature, the heat medium container 2 does not need to be heat-resistant.
- the heat medium storage container 2 is more preferably a container made of a synthetic resin such as polyethylene, polypropylene, polystyrene, or polyethylene terephthalate.
- the heat medium storage container 2 is preferably one that can be easily discarded after use, so that cross contamination can be prevented. Further, the heat medium storage container 2 is preferably a cylindrical structure having one end closed and the other end open so that it can be easily grasped by a human hand, and is perpendicular to the longitudinal direction of the cylindrical structure. More preferably, the diameter of the cross section is 5 mm or more and 200 mm or less. Further, it is preferable that the heating container be portable by human hands. As the heating vessel, for example, a commercially available centrifuge tube can be suitably used.
- the heat medium storage container 2 does not have to have a centrifugal tubular axially symmetric shape as shown in FIGS. 1 and 2, and various shapes such as a drug solution bottle and a plastic bottle can be suitably used. .
- the heating vessel does not have rigidity such as a bag-shaped structure, the deformation of the heating vessel itself should be minimized when circulating the heat medium in the exercise process, etc.
- the lid 6 may be any as long as it covers the opening 3 and seals the heat medium container 2. As the lid 6, one fitted into the opening 3, a screw wound type that is turned open and closed, and the like can be suitably used.
- the heat medium 4 only needs to be capable of exchanging heat with the biological sample in the biological sample container 5, and may be a fluid having a heat capacity capable of maintaining a predetermined temperature for a predetermined time.
- the heat medium 4 is preferably at least one selected from the group consisting of water, an isotonic solution and water in which an antibacterial agent is dissolved.
- the biological sample is a cell or a cell mass, if an isotonic liquid is used as the heat medium 4, the risk of damaging the cells can be reduced even if the heat medium 4 comes into contact with the biological sample.
- the heating container according to one embodiment of the present invention can be suitably used for the heating method according to one embodiment of the present invention. That is, the heating container according to one embodiment of the present invention can be used in the heating method according to one embodiment of the present invention, and can suppress damage to a biological sample, and can easily and safely heat a biological sample. it can.
- the heating container according to one embodiment of the present invention includes the position defining portion, the movement of the biological sample storage container in the heat medium storage container is limited. Can be prevented from moving greatly and colliding with a lid or the like, thereby damaging the container.
- a commercially available cryotube is used in a form in which the position defining portion has a pocket shape or a container shape and the heat medium and the biological sample housing container are not directly in contact with each other in the heat medium housing container. Therefore, even if a situation such as cracking of the surface or loosening of the cap due to freezing that can occur often occurs, the risk of the heat medium flowing into the biological sample container and contaminating the biological sample can be reduced.
- the heat medium storage container in the above may be a container made of a non-rigid (flexible) material.
- the container is excellent as a heat medium storage container in that the position defining portion is easily formed.
- the heat medium storage container of the specific example 1 is, for example, a bag made of a flexible material.
- the heat medium is enclosed inside the bag.
- the biological sample storage container is sandwiched between the folded bags at an arbitrary position (stored in the bag while being in contact with the outer surface of the bag).
- the bag is filled with a heating medium in advance, and the opening of the bag is closed in advance before coming into contact with the biological sample container. That is, the bag is manufactured by closing the entrance without housing the biological sample housing container therein. Easy to manufacture and fill with heat carrier because it can be manufactured by a general-purpose method such as a process of processing a vinyl or plastic bag from raw materials such as film or a process of closing a packed bag. It is.
- the bag of the specific example 1 may include a structure for mounting and fixing the biological sample container on the outer surface of the bag.
- the structure may be a string-like structure that forms a loop with the outer surface, or a sheet-like structure that forms a pocket with the outer surface.
- the bag of the specific example 1 including the structure can press the biological sample container more strongly against the outer surface of the bag. These function as a position determining unit.
- the bag can be further accommodated in a guide member such as a hollow cylindrical container. Since the cylindrical container maintains a certain shape, the easily deformable bag is easily maintained in a certain folded state (it is easy to reproduce almost the same contact state between the outer surface of the bag and the biological sample container). Since the cylindrical container suppresses the deformation of the bag, the circulation of the heat medium and the thermal contact with the biological sample storage container are hardly hindered by a change in the shape of the bag, and therefore, the heat of the heat medium is reduced. , Can be efficiently transmitted to the biological sample storage container. Vibration is likely to be applied to a cylindrical container having a fixed shape instead of an amorphous bag. It is preferable that air is further contained in the bag.
- the cylindrical container can be made of various rigid materials (paper, wood, resin or metal, etc.) in order to maintain a certain shape.
- the hollow guide member has a cylindrical shape as an example, the cross section may have a curved shape other than a circle or a polygonal shape.
- Another example of the bag is a bag having a depression formed on the outer surface of the bag.
- the depression has an opening provided on the outer surface of the bag, a hollow extending toward the inside of the bag, and a bottom closing the hollow at the opposite side of the opening.
- the depression has a shape capable of accommodating the biological sample accommodation container. It is very easy to provide a recess in a bag made of a flexible material. Therefore, the bag of Example 1 and the bag of Example 2 have the same advantages except for the presence or absence of the depression.
- the bag of the specific example 2 does not need to be folded because the biological sample storage container is stored in the recess. Simply closing the opening of the concave portion or fixing the biological sample container from outside the concave portion can prevent the biological sample container from dropping out of the concave portion in the bag of Example 2.
- the bag of the specific example 2 can realize the same advantages as the bag of the specific example 1 even if it is not housed in the guide member. Storing the bag of Example 2 in the guide member further enhances the advantages of the bag of Example 2.
- FIGS. 3 to 7 are schematic views showing a heating container according to still another embodiment of the present invention.
- 1030 in FIG. 3 shows a state before the biological sample storage container 21 is stored in the heating container 20, and 1031 in FIG. 3 shows a state after the biological sample storage container 21 is stored in the heating container 20. Is shown.
- the heat medium storage container 23 stores the heat medium 25
- the biological sample storage container 21 stores the biological sample 24.
- a fixing portion 22 for fixing the position of the biological sample storage container 21 in the heat medium storage container 23 is provided on the opening of the biological sample storage container 21.
- the fixing portion 22 has a portion protruding outside the outer edge of the biological sample container 21. Therefore, when the biological sample storage container 21 is stored in the heat medium storage container 23, the protruding portion comes into contact with the opening 26 of the heating container 20, and the biological sample storage container 21 enters inside from the position. By preventing this, the position of the biological sample storage container 21 in the heat medium storage container 23 is fixed.
- the portion of the fixing portion 22 protruding outside the outer edge of the biological sample storage container 21 may be a wing-like structure.
- the fixing unit 22 may be provided on a lid that seals the biological sample storage container 21, or the fixing unit 22 itself may function as a lid of the biological sample storage container 21. .
- the biological sample is stored in the heat medium storage container 23 when the heating container 20 is subjected to a movement process such as shaking.
- the movement of the storage container 21 can be suppressed. Accordingly, mechanical collision between the biological sample storage container 21 and the heat medium storage container 23 can be avoided.
- the biological sample storage container 21 is lifted by its own buoyancy to hinder thermal contact with the heat medium 25.
- the fixing portion 22 also functions as a lid for closing the opening 26 of the heating container 20. Since the fixing part 22 functions as a lid of the heating container 20, the outer diameter of the fixing part 22 is larger than the outer diameter of the opening 26. Note that a lid for the heating container 20 may be separately provided. Further, when the outer diameter of the biological sample storage container 21 is small and the heat transfer characteristic from the surface to the inside of the biological sample storage container 21 is good, a motion process such as shaking at the time of heating is unnecessary or the heat medium 25 is not used. It is not necessary to separately provide the lid of the heating vessel 20 if the movement step such as shaking to the extent that the liquid level does not greatly fluctuate is acceptable.
- ⁇ Modification 2> 4 indicates a state before storing the biological sample storage container 31 in the heating container 30, and 1041 in FIG. 4 indicates a state after storing the biological sample storage container 31 in the heating container 30. Is shown.
- 1040 in FIG. 4 a heat medium 35 is stored in the heat medium storage container 33, and a biological sample 34 is stored in the biological sample storage container 31.
- the heating container 30 differs from the heating container 20 in that a donut-shaped fixing portion 36 is provided.
- the biological sample container 31 is sealed by a lid 32, and the lid 32 projects at least partially outside the outer edge of the biological sample container 31.
- the protruding portion of the lid 32 comes into contact with the fixing portion 36, so that the biological sample container 31 does not enter inside from that position. I have.
- the fixing unit 36 contacts the opening 38 of the heating container 30, and the heat medium of the biological sample storage container 31 The position in the storage container 33 is fixed.
- the outer diameter of the fixing portion 36 is larger than the outer diameter of the opening 38, and the fixing portion 36 functions as a lid of the heating vessel 30, but may have a separate lid.
- the biological sample in the heat medium storage container 33 is subjected to a movement process such as shaking the heating container 30.
- the movement of the sample storage container 31 is suppressed, and mechanical collision between the biological sample storage container 31 and the heat medium storage container 33 can be avoided. Further, there is no problem that the biological sample container 31 is lifted up by its own buoyancy to hinder thermal contact with the heat medium 35.
- a small-diameter container such as a microtube for storing a small amount of a biological sample such as a template, a primer, or a protein extract is used as a biological sample storage container.
- a tubular projection 37 may be provided at the lower end of the fixing portion 36, thereby preventing the fixing portion 36 from slipping off from the heating container 30. it can.
- ⁇ Modification 3> 5 indicates a state before storing the biological sample storage container 41 in the heating container 40
- 1051 in FIG. 5 indicates a state after storing the biological sample storage container 41 in the heating container 40. Is shown.
- a heat medium 45 is stored in the heat medium storage container 43
- a biological sample 44 is stored in the biological sample storage container 41, which is sealed by the lid 42.
- the heating container 40 differs from the heating container 20 in that a contact member 48 is provided in the heat medium storage container 43.
- the contact member 48 is provided on the side of the opening 49 in the heat medium container 43, and the outside surface of the biological sample container 41 abuts on the contact member 48 so that the biological sample container 41 is moved from that position. So that they cannot enter the inside.
- the contact between the contact member 48 and the outer surface of the biological sample container 41 may be point contact, line contact, or surface contact. It is preferable that the surface area of contact with the metal be larger.
- the biological sample storage container 41 is stored in the heat medium storage container 43 so as to abut on the contact member 48, and the heating container 40 is closed by the lid 46.
- the lid 46 has a contact member 47 that contacts the biological sample container 41 when the heating container 40 is closed.
- the contact member 47 comes into contact with the biological sample storage container 41, and the lid 46 presses the biological sample storage container 41 from above.
- the biological sample storage container 41 can be fixed in the heat medium storage container 43. Accordingly, mechanical collision between the biological sample storage container 41 and the heat medium storage container 43 can be avoided. Further, there is no problem that the biological sample container 41 is lifted up by its own buoyancy to hinder thermal contact with the heat medium 45.
- ⁇ Modification 4> 6 indicates a state before storing the biological sample storage container 51 in the heating container 50
- 1061 in FIG. 6 indicates a state after storing the biological sample storage container 51 in the heating container 50. Is shown.
- a heat medium 55 is stored in the heat medium storage container 53
- a biological sample 54 is stored in the biological sample storage container 51.
- the heating container 50 is different from the heating container 20 in that the heating container 50 includes a cover 52.
- the biological sample storage container 51 is sealed with a lid 56, the diameter of the lid 56 is larger than the diameter of the opening of the biological sample storage container 51, and the outer edge of the lid 56 is located outside the outer edge of the biological sample storage container 51. It is protruding.
- a skirt-shaped cover 52 that covers the periphery of the biological sample storage container 51 with the biological sample storage container 51 sealed is provided near the outer edge of the lid 56 that is in contact with the biological sample storage container 51.
- the cover 52 has a fitting portion 57 on the inner surface on the open end side.
- a fitting portion 58 is provided on the outer surface of the heat medium container 53 near the opening 59.
- the fitting portion 57 and the fitting portion 58 may be configured by, for example, screw surfaces that can be fitted to each other.
- the inner diameter of the cover 52 is larger than the outer diameter of the opening 59 by the fitting portion 57 and the fitting portion 58. Therefore, as shown by 1061 in FIG. 6, when the biological sample storage container 51 is stored through the opening 59 of the heat medium storage container 53, a part of the cover 52 covers the heat medium storage container 53, and the fitting portion 57 is The biological sample storage container 51 is fitted in the fitting portion 58, and the position of the biological sample storage container 51 in the heat medium storage container 53 is fixed. Accordingly, mechanical collision between the biological sample storage container 51 and the heat medium storage container 53 can be avoided. Further, there is no problem that the biological sample storage container 51 is lifted by its own buoyancy, thereby preventing thermal contact with the heat medium 55.
- 1070 in FIG. 7 shows a state before storing the biological sample container 61 in the heating container 60
- 1071 in FIG. 7 shows a state after storing the biological sample container 61 in the heating container 60. Is shown.
- the heat medium 65 is stored in the heat medium container 63
- the biological sample 64 is stored in the biological sample container 61.
- the heating container 60 is different from the heating container 20 in that the heating container 60 includes a position defining unit 68.
- the heating container 60 includes a position defining portion 68 provided so as to cover the opening 69.
- the position defining section 68 accommodates the biological sample container 61 inside, and is provided such that an entrance portion thereof is caught by the opening 69 of the heat medium container 63.
- the position defining section 68 is provided so that the biological sample 64 and the heat medium 65 can exchange heat via the position defining section 68 when the biological sample storage container 61 is stored. Since the heat medium 65 does not enter the position defining section 68, the biological sample container 61 and the heat medium 65 do not come into direct contact with each other.
- the inner surface of the position defining portion 68 is shaped along the outer surface of the biological sample container 61, and when the biological sample container 61 is stored, the inner surface of the position defining portion 68 and the outer surface of the biological sample container 61 Are in physical contact.
- the position defining section 68 is a rigid structure, and may be, for example, a metal container, a plastic container, or the like.
- the position defining portion 68 is a rigid structure, the risk of breakage is low and cleaning is easy. However, compared to the position defining portion formed of the resin film 11 shown in FIG. There is a possibility that the adhesiveness to the sample container 61 is low, and the heat conduction characteristics are reduced. In order to prevent such a decrease in heat conduction characteristics, the position defining portion 68 may be formed of a material having high thermal conductivity such as aluminum, or a material having high thermal conductivity and plasticity may be formed on the entire inner surface of the position defining portion 68. It is conceivable to newly provide a member constituted by the above.
- a liquid ceramic paint such as Shellac ⁇ (registered trademark) can be exemplified.
- Shellac ⁇ registered trademark
- First Paste registered trademark
- the heating container 60 further includes a lid 66, and seals the heating container 60 by closing an opening of the position defining portion 68 in which the biological sample container 61 is stored.
- the lid 66 has a contact member 67 that is fitted into the opening of the position defining portion 68 when the heating container 60 is sealed, and that contacts the biological sample container 61.
- the contact member 67 abuts on the biological sample storage container 61, so that the lid 66 presses the biological sample storage container 61 from above.
- the biological sample storage container 61 can be prevented from jumping out when subjected to a movement process such as shaking of the biological sample 60, and the biological sample storage container 61 and the position defining portion 68 can be more firmly contacted.
- a kit for heating a biological sample according to one embodiment of the present invention includes a heat medium storage container for storing a heat medium and a biological sample storage container.
- the heat medium storage container has a position defining portion for specifying the position of the biological sample storage container, and the biological sample storage container is for storing a biological sample.
- a kit for heating a biological sample according to a preferred embodiment of the present invention is a heating container for housing a heat medium, and is for housing a biological sample, and can be stored in the heating container.
- the kit for heating a biological sample may further include a washing solution for washing the biological sample, and the kit for heating the biological sample may be a storage solution for storing the washed biological sample. May be provided.
- FIG. 8 is a schematic diagram illustrating a kit (heating kit) for heating a biological sample according to one embodiment of the present invention.
- the heating kit 70 is an example of a package kit for bringing a regenerative medicine product into a clean environment such as a hospital room or an operating room.
- the heating kit 70 includes a biological sample container package 71 in which a biological sample container 72 is enclosed, a heating container package 73 in which a heating container 74 is enclosed, and a cleaning container 76.
- a set of a sealed cleaning container package 75 and a storage container package 77 in which a storage container 78 is sealed is provided.
- the biological sample storage container 72 stores a biological sample in advance, and the biological sample storage container 72 may be sterilized and stored in a sealed container.
- the biological sample storage container 72 the biological sample storage container used in the above-described heating method according to one embodiment of the present invention can be used.
- a heating medium is accommodated in the heating container 74.
- the heating container the above-described heating container according to one embodiment of the present invention can be used.
- the cleaning container 76 contains a cleaning liquid for cleaning a heated biological sample. Examples of the washing solution include an isotonic solution such as Ringer's lactate solution or PBS.
- the storage container 78 contains a storage solution for storing the washed biological sample. Examples of the storage solution include Ringer's lactate solution and PBS.
- a commercially available centrifuge tube with a lid attached may be used.
- a biological sample container 72 containing a biological sample in advance cannot be subjected to ⁇ -ray sterilization, but a heating container package 73 containing a heating container 74 and a cleaning container package containing a cleaning container 76.
- the storage container package 75 in which the storage container 75 and the storage container 78 are sealed is previously packaged and sterilized by ⁇ -ray sterilization or the like.
- the heating kit 70 may include gripping means such as tweezers for removing the biological sample from the biological sample container 72, and these are also packaged and subjected to ⁇ -ray sterilization.
- kits 70 a biological sample stored at a low temperature is previously stored in the biological sample holding container 72, and the kit 70 may further include a cold-retaining means for maintaining a low temperature state of the biological sample.
- a cool keeping mechanism or a cool keeping agent may be used, and the biological sample holding container 72 containing the biological sample may be stored in the cool keeping agent.
- the method of using the heating kit 70 will be described by taking as an example a case where the biological sample is mesenchymal stem cells (MSC).
- MSC mesenchymal stem cells
- the biological sample container 72 taken out by opening the biological sample container package 71 is taken out by opening the heating container package 73. It is stored in the container 74.
- the heating container 74 that accommodates the biological sample container 72 is heated to quickly heat the MSC, thereby sufficiently melting the MSC.
- the heated MSC is transferred to the cleaning container 76 taken out by opening the cleaning container package 75 for cleaning.
- the washed MSC is transferred to a storage container 78 taken out by opening the storage container package 77 and stored until immediately before transplantation.
- heating condition evaluation The heating conditions in the heating method according to one embodiment of the present invention were evaluated in a simulated environment.
- the heating method of the present invention is referred to as “Tube in Tube method”.
- a simulated environment heating conditions were evaluated using not a cell but a frozen sample obtained by freezing a 1 mL cryopreservation solution as a biological sample.
- the composition of the cryopreservation solution is 90% (v / v) STK®2 (cytokine free) + 10% (v / v) DMSO (Wako 031-24051).
- a centrifuge tube having no position defining part was used as a heating vessel.
- cryopreservation solution was stored in a freezing vessel (cryogenic vial: WHEATON, Cat. W98865) as a biological sample storage container, and frozen under a deep freezer at ⁇ 80 ° C.
- the frozen vessel was stored in a centrifuge tube (50 mL centrifuge tube: Sumitomo Bakelite Co., Ltd. Cat. MS-56501) containing the heat medium having the temperature and amount shown in Table 1, and the heat medium was circulated by the method shown in Table 1, respectively. Then, the biological sample in the frozen vessel was heated and thawed.
- thawing was performed by a water bath method using a 37 ° C. water bath. Thawing using a water bath at 37 ° C. shows the most preferable result among the conventional thawing methods in the study of the present inventors.
- Table 1 shows the thawing conditions of the frozen sample and the results of thawing under each thawing condition (thawing time).
- condition 0 when the condition 0 is compared with the conditions 1 and 2, compared with the water bath method at 37 ° C., in the Tube in Tube method at 37 ° C., when the heat medium is allowed to stand without shaking, A 1.6-fold thawing time was required.
- condition 2 when the heat medium was shaken to circulate, the thawing was possible in the same thawing time as in the water bath method. From these results, by shaking the heating vessel and circulating the heat medium, even with a simple configuration such as the heating vessel according to one embodiment of the present invention, when using a conventional water bath It was shown that it can be thawed equally.
- the temperature of the heat medium is about room temperature (24 ° C.), and the condition 3 for shaking the heat medium requires a thawing time 1.5 times as long as the conditions 0 and 2.
- the reason for this is that, as in the case of the order estimation in the column of the above embodiment, a theoretical calculation was performed on the temperature change of the heat medium when there was no heat exchange with the outside. Was found to decrease by 13 ° C. That is, when the temperature of the heat medium is 24 ° C., it is considered that it is necessary to take sufficient heat capacity of the heat medium.
- FIG. 9 shows an example of a method of inversion mixing, and shows a state in which a centrifuge tube in which a frozen vessel is not inserted is inverted.
- FIG. 9 shows an example of the operation, the operator does not take necessary measures such as gloves, but it is preferable to take necessary protection and contamination prevention measures at a clinical site or the like.
- the centrifuge tube 82 is gripped by the hand 81 of a person and twisted with the wrist to cause overturning and mixing.
- 9 shows a state in which the lower end of the centrifuge tube 82 is swung in a counterclockwise direction
- 1091 in FIG. 9 shows a state in which the lower end of the centrifuge tube 82 is swung in a clockwise direction.
- a dashed line 83 indicating the central axis of the centrifuge tube 82 and a broken line 84 indicating the vertical direction are annotations described for convenience of explanation, and are not actual components.
- the angle between the broken line 84 and the dashed line 83 (the counterclockwise direction is defined as the positive direction of the angular dimension) is approximately ⁇ 30 ° in 1090 in FIG. 9 and approximately 120 ° in 1091 in FIG.
- the centrifuge tube swings the centrifuge tube between approximately ⁇ 30 ° and approximately 120 °, and the swing angle is approximately 150 °.
- the thawing could be performed at almost the same speed (1.1 times) as the 37 ° C. water bath method of the condition 1.
- the liquid volume of the heat medium is sufficient, and the temperature of the heat medium is reduced to room temperature by performing upside-down mixing that can sufficiently generate forced heat convection.
- a thawing speed almost equal to that of the conventional method using a 37 ° C. water bath could be realized.
- FIG. 10 shows an example of a heat medium storage container and a biological sample storage container.
- a tube 91 serving as a heat medium storage container, a resin film 93 for forming a position defining portion, a lid 94, and a frozen vessel 92 were used as a biological sample storage container. Note that a 50 mL centrifuge tube was used as the tube 91.
- the resin film 93 has a bag shape, and the frozen vessel 92 is stored in the bag, and the resin film 93 is dimensioned to fit the frozen vessel 92. Specific dimensions of the resin film 93 will be described with reference to FIG. As can be seen from a comparison with the scale 102 (0.5 cm per square), the resin film 93 has a length of about 4.5 cm and a width of about 1.5 cm. Here, the resin film 93 was formed by cutting a part of a finger part of an experimental rubber glove called Labendonitrile (registered trademark).
- FIG. 12 shows a state where the heating container 111 is gripped by a human hand 112.
- the tube 91 has a shape symmetrical with a dashed line 115 indicating the central axis thereof, but a tube having a shape that is not axially symmetric may be used. Note that the dashed line 115 is an annotation described for convenience of explanation.
- the tube 91 has an opening 116 and a bottom 113. Although a tube with a scale is used, a tube without a scale may be used.
- FIG. 13 shows an enlarged view of the frame 114 in FIG.
- Approximately 40 mL of the heat medium is stored in the tube 91, and the liquid level is indicated by a broken line 123.
- the resin film 93 was attached such that the bag-shaped entrance was widened and hooked on the opening 116 of the tube 91, and a parafilm was wound around the frame 122 to fix the resin film 93.
- the position of the resin film 93 attached to the opening 116 is indicated by a dashed line 121.
- the dashed line 121 and the dashed line 123 are annotations described for convenience of explanation.
- the heating conditions of the biological sample were examined using the heating container 111 thus manufactured.
- the heating method of the present invention using the heating vessel 111 is referred to as a non-contact Tube-in-Tube method.
- a non-contact Tube was When the biological sample was heated and thawed by the in Tube method, the thawing time varied (170 seconds to 220 seconds). This thawing time was equivalent to 1.1 to 1.4 times the thawing time of Condition 4 in Table 1, and the thawing time was prolonged.
- there is a problem in that there is a variation of about one minute in total Thus, it was found that there was a problem in reproducibility of the thawing characteristics. Therefore, as a result of further study, it was found that the thawing time becomes longer when there is an air layer between the resin film 93 and the freezing vessel 92 or when the resin film 93 has slack.
- FIG. 15 shows a state before the frozen vessel 92 is pushed into the resin film 93
- 1151 in FIG. 15 shows a state where the frozen vessel 92 is pushed into the resin film 93. Note that the frozen vessel 92 was pushed into the resin film 93 by a human hand 112.
- the lower end of the resin film 93 before the frozen vessel 92 was pushed was at the position shown by the broken line 602, but when the frozen vessel 92 was pushed by the human hand 112, the lower end of the resin film 93 was at the position shown by the broken line 603. , About 1 cm frozen vessel 92 was pushed in. By pushing the frozen vessel 92 in this way, the adhesion between the frozen vessel 92 and the resin film 93 was improved against the elastic resistance of the resin film 93.
- the thaw was able to be thawed in a thaw time equivalent to that of a 37 ° C. water bath even when the temperature of the heat medium was 22 ° C.
- FIG. 15 shows an example in which the freezing vessel 92 is pushed in by a human hand 112 in order to clearly show the pushing amount of the freezing vessel 92, but actually, the freezing vessel 92 is pushed by the lid 94. , The frozen vessel 92 was pushed into the resin film 93.
- FIG. 15 shows the operation for the purpose of illustration, the operator does not take necessary protective measures such as gloves, but it is preferable to take necessary protective and contamination preventive measures at a clinical site or the like.
- the washed synovial tissue is cut into small pieces of 5 mm or less using sterile scissors on a container, and further suspended in DMEM containing gentamicin. Then, the synovial tissue pieces are placed in a 50 mL centrifuge tube. Collected. Thereafter, centrifugation was performed at room temperature at 1500 rpm for 5 minutes, and the supernatant was removed.
- STK registered trademark 1 (serum-free medium for establishment of primary MSC, DS Pharma Biomedical Co., Ltd.) was added, and the seeding density was adjusted to 150 mg so that the seeding density was 2.5 mg (synovial tissue piece) / cm 2 (culture dish surface area).
- the cells were seeded on a 2 dish (Sumitomo Bakelite Co., Ltd.), and cultured at 37 ° C. for 14 days at a CO 2 concentration of 5% (medium exchange was performed on days 5, 8, and 11).
- the cells pelleted down from the single cell suspension were suspended again in the washing medium, and the number of cells was counted by trypan blue staining.
- the cells were seeded on a 150 cm 2 dish (Sumitomo Bakelite Co., Ltd.) with STK (registered trademark) 2 at a concentration of 5000 cells / cm 2, and cultured at a CO 2 concentration of 5% at 37 ° C. for 5 days (on the third day).
- the medium was replaced) and the same operation was repeated up to the third passage.
- the cells were further centrifuged at 1500 rpm for 5 minutes at room temperature, pelleted down, and the supernatant was removed.
- the cells were suspended with STK (registered trademark) 2, and the number of cells was counted by trypan blue staining.
- STK registered trademark 2 2
- cells were seeded at a high seeding density of 40 ⁇ 10 4 cells / cm 2 on a 6-well plate (Sumitomo Bakelite Co., Ltd.). Therefore, the number of cells at the time of high-density seeding is 3.68 million cells per MSC.
- the cells were cultured at 37 ° C. in a 5% CO 2 incubator for 7 days. Medium exchange was performed on the third day and the fifth day.
- MSCs strain 1, strain 2, strain 3 of three strains (different donors for different strains) established based on the above 2-1 were obtained according to the method of 2-2 to 2-4 above.
- GMSC registered trademark 1 derived from each strain was divided into 4 groups of a non-frozen group, a thawing method A group, a thawing method B group, and a thawing method C group (thus there are a total of 12 groups of 3 strains x 4 groups).
- the non-frozen group gMSC (registered trademark) 1 was digested and decomposed to a single cell suspension according to the above 2-5, and the number of cells was measured under the conditions of 2-5.
- Thawing methods gMSC®1 of Groups A to C were all frozen and stored according to the above 2-6, and then thawed for each group by the following heating method: -Thawing method group A: Thawing using a 37 ° C water bath for 2.5 minutes.-Thawing method group B: Thawing by inversion mixing at 22 ° C (room temperature) at about 60 rpm for 3 minutes by Tube in Tube method. Group C: Thawed for 4.5 minutes in a heat block set at 37 ° C.
- each of these gMSC (registered trademark) 1 was digested and decomposed into a single cell suspension according to the above 2-5, and the number of cells was measured by the method of 2-5.
- the thawing results for strain 1 are shown in FIG. 16, the thawing results for strain 2 are shown in FIG. 17, and the thawing results for strain 3 are shown in FIG.
- the item axes of the bar graphs shown in FIGS. 16 to 18 correspond to “non-frozen group”, “thawing method group A”, “thawing method group B”, and “thawing method group C” from the left.
- the white bar on the left indicates “total number of cells per gMSC (registered trademark) 1 (1 drop)”
- the black bar on the right indicates “raw cells per gMSC (registered trademark)”. Cell number.
- the error bar in each bar represents the standard deviation.
- the total cell count means the total number of cells (regardless of viability) recovered by the method of 2-5.
- the number of viable cells means the number of surviving cells among the cells recovered by the above method 2-5.
- "recovered cells” are cells that can be recognized as cells in a cell counting method such as a normal cell counter, and include residues that have been damaged in the process of freezing, thawing, collecting, etc. Absent.
- the comparison target of the total cell is the total cell
- the comparison target of the living cell is the living cell.
- the Tube @ in @ Tube method (the thawing method B group) and the 37 ° C. water bath method (the thawing method A group) were compared with the 37 ° C. heat block method (the thawing method C group) in comparison with the total cell number and cell viability after thawing. It was found that the number of cells tended to be large. From the above results, the 37 ° C. heat block method (thawing method group C) causes more damage to cells than the 37 ° C. water bath method (thawing method group A) and the Tube-in-Tube method (thawing method group B). It turns out that there is a tendency.
- the number of cells replated in each well is 50,000 each.
- the cells replated in each well are all cells recovered from one gMSC (registered trademark) 1, and are therefore cells derived from the same strain and thawed by the same heating method.
- the conditions for monolayer culture were almost the same as in 2-2 above, and the culture period was fixed at 5 days.
- the proliferative capacity of cells of strain 1 is shown in FIG. 19, the proliferative capacity of cells of strain 2 is shown in FIG. 20, and the proliferative capacity of cells of strain 3 is shown in FIG.
- the legends of the bar graphs shown in FIGS. 19 to 21 are the same as those in FIGS. 16 to 18. From these results, the cells recovered by the Tube ⁇ in ⁇ Tube method (the thawing method B group) were replated and subcultured for one passage, and when the cells were recovered, both the total cell number and the viable cell number were determined by the thawing method A. It was found that the number was larger than when cells collected in the group and the thawing method group C were used.
- thawed using the Tube ⁇ in ⁇ Tube method (thaw method B group) have a higher proliferative capacity immediately after re-seeding after thawing than cells thawing using the other two methods, that is, immediately after thawing. It was found that the rise was at least equal or higher.
- the non-frozen group gMSC (registered trademark) 1 was digested and decomposed into a single cell suspension according to the above 2-5, and the cell number was measured under the conditions of the above 2-5.
- gMSC Freezing and thawing of gMSC (registered trademark) 1) All gMSCs® 1 assigned to thawing methods A or D were frozen according to 2-6 above and then thawed for each group by the following warming method: Thawing method group A: Thawing using a 37 ° C. water bath for 2.5 minutes Thawing method group D: Non-contact Tube-in-Tube method: Thaw by inverting and mixing at 22 ° C. (room temperature) at about 60 rpm for 3 minutes.
- each of these gMSC (registered trademark) 1 was digested and decomposed into a single cell suspension according to the above 2-5, and the number of cells was measured by the method of 2-5.
- FIG. 22 shows the total cell number and the viable cell number after thawing.
- the legend in FIG. 22 is substantially the same as that in FIGS. 16 to 21.
- the solid line indicates the total cell number (average value) of the thawing method D group
- the dotted line indicates the thawing method D group.
- the number of live cells (average value) of the group is shown. From the results shown in FIG. 22, when the cells were thawed by the non-contact Tube-in-Tube method (the thawing method D group), compared with the 37 ° C. water bath method (the thawing method A group), both the total cell number and the viable cell number were compared. Was found to be equal or better.
- the number of cells replated in each well is 50,000 each.
- the cells replated in each well are all cells recovered from one gMSC (registered trademark) 1, and are therefore cells derived from the same strain and thawed by the same heating method.
- the conditions for monolayer culture were almost the same as in 2-2 above, and the culture period was fixed at 5 days.
- FIG. 23 shows the proliferation ability of the cells after thawing.
- the legend of the bar graph shown in FIG. 23 is the same as that of FIG. From these data, it was found that cells thawed using the non-contact Tube in Tube method (thawing method group D) had the same rise as cells thawed using the 37 ° C water bath method (thawing method group A). Was obtained.
- the washed adipose tissue was cut into small pieces of 5 mm or less using sterilized scissors, and digested with a 0.4% collagenase solution (Worthington Biochemical Corporation) at 37 ° C. for 1.5 hours. Thereafter, the mixture was filtered through a 100 ⁇ m mesh (Greiner Bio-One International GmbH) and collected in a new 50 mL centrifuge tube. After centrifugation, the supernatant was removed, and the cells were suspended in STK (registered trademark) 1 (serum-free medium for establishment of primary MSC, DS Pharma Biomedical Co., Ltd.).
- STK registered trademark
- a part of the cell suspension was stained with 0.4% trypan blue (Thermo Fisher Scientific Inc.), and the number of living cells and the number of dead cells were counted.
- the cell suspension was diluted with STK (TM) 1 to seeding density of 5000 cells / cm 2 (culture dish surface), 150 cm 2 dish were seeded into (Sumitomo Bakelite Co., Ltd.) on, CO 2 concentration 5%, 37 ° C. For 14 days (medium exchange was performed on days 5, 8, and 11).
- MSCs derived from human fat A31, P4, hereinafter referred to as “ADMSC” were cultured by the same operation as described in items 2-2 and 2-3.
- the aforementioned ADMSC is STK (registered trademark) 2 medium, and human dermal fibroblasts (NHDF, P14; hereinafter, referred to as “NHDF”) obtained from Lonza Japan are DMEM medium containing 10% FBS, respectively.
- the cells were cultured at a CO 2 concentration of 5% at 37 ° C.
- the cell suspension after collection (stripping and washing) was transferred to a 2 mL cryovial containing 1 mL of a cell preservation solution.
- the cell preservation solution for ADMSC is CELLBANKER2 (Nippon Zenyaku Kogyo Co., Ltd.), and the cell preservation solution for NHDF is CellBanker1 (Nippon Zenyaku Kogyo Co., Ltd.).
- Method D group (N 3) was obtained. Details of “thawing method A” and “thawing method D” are as described in item 3-1.
- An equal volume of the cell suspension was collected from each sample of the thawing method group A and the thawing method group D, and the collected cell suspensions were stained with trypan blue, and contained in each stained cell suspension. The total number of living cells and the number of living cells were counted.
- FIG. 24 shows the total cell number and the viable cell number after thawing for NHDF (upper panel) and ADMSC (lower panel), respectively.
- the thawing method D group showed almost no decrease in the total cell number and the viable cell number as compared with the thawing method A group.
- the 6-well plate seeded with NHDF was placed in a culture condition of 5% CO 2 and 37 ° C. for 5 days.
- the 6-well plate seeded with ADMSCs was placed in a culture condition of 5% CO 2 and 37 ° C. for 7 days.
- FIG. 25 shows the total cell number and the viable cell number after thawing and culturing for NHDF (upper panel) and ADMSC (lower panel), respectively.
- the thawing method D group did not impair the cell growth ability, and showed slightly higher total cell numbers and viable cell numbers (average value) than the thawing method A group.
- the thawing method D is capable of converting a cryopreserved homogeneous cell population (for example, an established cell line) to at least as effective as the conventional thawing method A (live cells). (Number and proliferation ability).
- the present invention can provide safe and useful transplantation treatment materials, and thus can be suitably used for regenerative medicine such as transplantation treatment.
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Abstract
Description
本発明の一態様に係る加温方法は、生体試料の加温方法であって、熱媒体が収容された熱媒体収容容器に、生体試料が収容された生体試料収容容器を収容する収容工程と、前記熱媒体が前記熱媒体収容容器の外に漏れないように前記熱媒体収容容器の前記熱媒体の入れ口を閉鎖する閉鎖工程と、前記熱媒体の入れ口が閉鎖した前記熱媒体収容容器内の前記熱媒体を運動させる運動工程と、を含む。
収容工程において、熱媒体が収容された熱媒体収容容器に、生体試料が収容された生体試料収容容器を収容する。本明細書において「容器AにBを収容する」は、(場合1)容器Aの内部に空間を有している容器Aの、当該空間にBを入れること、又は(場合2)容器Aの外表面と接するBを、容器Aによって包むことを意味する。(場合2)は、容器Aが変形可能な材質によって作製されていることを、さらに意味している。以下では、収容工程を、(場合1)を例に詳述する。
熱媒体収容容器は、内部に熱媒体を収容するものであり、熱媒体の入れ口となる開口部を有している。熱媒体収容容器の一例について図1を参照して説明する。図1は、本発明の一態様に係る加温方法で用いる加温容器を示す模式図である。加温容器1は、熱媒体収容容器2を有している。熱媒体収容容器2には、熱媒体4を注入するための開口部3が設けられている。また、熱媒体収容容器2には生体試料が収容された生体試料収容容器5が収容されている。図1は、後述する閉鎖工程において開口部3を蓋6により閉鎖した状態を例として示している。
上述の通り、熱媒体収容容器の一態様は、その外表面に生体試料収容容器を収容する(場合2)。(場合2)では、より正確には、熱媒体収容容器は生体試料収容容器を包む。したがって、(場合2)の熱媒体収容容器は、柔軟な材質によって作製されている容器である。
ここで、生体試料と熱媒体との熱交換により、熱媒体にどの程度の温度変化が生じるのかに基づいて、下記のとおり、熱媒体の量、熱媒体収容容器の容量をオーダーエスティメーションすることができる。このようなオーダーエスティメーションの結果に基づき、熱媒体の種類、量、温度、熱媒体収容容器の容積等を設計してもよい。
生体試料は固体であるため、その比熱は、氷の比熱(2.1kJ K^-1kg^-1)に近似すると考えられる。従って、当該過程においては、
(熱エネルギー)=(質量)×(比熱)×(温度変化)・・・(式1)
に従って、生体試料は熱媒体から
Q1[kJ]=0.001×2.1×80=0.168・・・(式2)
の熱を受け取ることになる。この結果、熱媒体は、Q1[kJ]の熱を奪われることとなり、
(温度変化)=(熱エネルギー)/((質量)×(比熱))・・・(式3)
に従って、温度変化を起こすと考えられる。熱媒体は液体であるため、その比熱は、水の比熱(4.2kJ K^-1kg^-1)に近似すると考えられる。したがって、
ΔT1[K]=(-0.168)/(0.04×4.2)=-1.0・・・(式4)
の温度変化が熱媒体に生じる。
生体試料が固体から液体に変化する過程では、融解熱に相当する熱エネルギーを熱媒体から得る必要があり、必要な熱エネルギーは、
(熱エネルギー)=(質量)×(融解熱)・・・(式5)
により算出される。融解熱は、氷の融解熱(335kJ kg^-1)に近似すると考えられるため、この過程において、生体試料は熱媒体から
Q2[kJ]=(0.001)×(335)=0.335・・・(式6)
の熱を受け取ることになる。したがって熱媒体は、Q2の熱を奪われることになる。この結果、熱媒体は、上記式4と同様に、
ΔT2[K]=(-0.335)/(0.04×4.2)=-1.99・・・(式7)の温度変化が熱媒体に生じる。
熱量保存の法則に従えば、温度T、質量M、比熱Cの流体と、温度T’、質量M’、比熱C’の流体とを接触させた場合の平衡温度T∞は、相変化が起きないと仮定した場合、
T∞=(M×C×T+M’×C’×T’)/(M×C+M’×C’)・・・(式8)
により求められる。ここで、生体試料と熱媒体とが同一の比熱を有すると近似できるため、絶対零度をT0[℃]と表記すると0℃=-T0[K]となり、上記式8は以下のように簡略化することができる。
=(M×(T[℃]+T0[K])+M’×(T’[℃]+T0[K]))/(M+M’)
=(M×T[℃]+M’×T’[℃])/(M+M’)+T0[K]・・・(式8’)
したがって、さらに生体試料の過程iiiにおける初期温度が0℃であれば、
T∞[℃]=(M×T[℃]+M’×T’[℃])/(M+M’)
=(M[g]×T[℃])/(M[g]+M’[g])・・・(式8’’)となり、この過程の平衡温度は、
T∞[℃]=(21×40+0×1)/(40+1)=20.5・・・(式8’’’)となる。したがって、この過程において、熱媒体の温度変化ΔT3は、
ΔT3[K]=20.5-21=-0.5・・・(式9)
となる。また、この過程において、生体試料は、式1と同様に、
Q3[kJ]=0.001×4.2×20.5=0.086・・・(式10)
の熱を熱媒体から受け取ることになり、すなわち熱媒体はQ3[kJ]の熱を奪われることになる。この程度の温度変化であれば、熱の変化も微小であるため、外部環境との熱交換も発生し得る実使用時には無視できる温度変化である。
閉鎖工程において、収容工程後、熱媒体が熱媒体収容容器の外に漏れないように熱媒体収容容器の熱媒体の入れ口を閉鎖することが好ましい。例えば、熱媒体収容容器の熱媒体の入れ口を蓋で覆うことによって閉鎖することができる。熱媒体収容容器の熱媒体の入れ口を閉鎖することで、位置規定部等が、熱媒体の熱媒体収容容器からの流出を封止する機能を備えない(例えば後述の図5参照)形態や、そもそも位置規定部を備えない(例えば図1参照)形態においても、後述する運動工程において熱媒体収容容器ごと運動させた際にも熱媒体の入れ口から熱媒体及び生体試料収容容器が飛び出さず、作業者及び作業場の汚染を防ぐことができる。
運動工程において、熱媒体の入れ口が閉鎖した前記熱媒体収容容器ごと運動させることが好ましい。運動工程においては、熱媒体収容容器内で熱媒体が対流し、熱媒体収容容器内を熱媒体が循環するように、生体試料収容容器を装着した状態で熱媒体収容容器ごと運動させる。これにより、熱媒体と生体試料との(生体試料収容容器を介した)熱交換がより効率よく行なわれ、生体試料をより短時間で加温することができる。
本発明の他の一態様に係る加温容器は、生体試料の加温容器であって、生体試料が収容される生体試料収容容器を収容し、かつ熱媒体が内部に収容される、熱媒体収容容器と、当該熱媒体収容容器に設けられた、前記生体試料収容容器の位置を規定するための位置規定部とを備えている。
具体例1の熱媒体収容容器は、例えば、柔軟な材料によって作製されている袋である。熱媒体は、当該袋の内部に封入されている。生体試料収容容器は、任意の位置で折りたたまれた袋に挟まれる(袋の外表面に接した状態で、当該袋に収容される)。袋には予め熱媒体が充填されており、かつ袋の入れ口は生体試料収容容器と接する前に予め閉鎖されている。つまり、袋は、その内部に生体試料収容容器を収容せずに、入れ口を閉鎖することによって作製される。ビニールやポリ袋をフイルム等の原材料からから袋状の構造加工する工程や、ものを詰めた袋を閉じる工程等で汎用的に使われる手法で作製可能であるため作製や熱媒体の充填が容易である。
袋の他の例として、当該袋の外表面に窪みが形成されている袋が挙げられる。当該窪みは、当該袋の外表面に設けられている開口部、当該袋の内部に向かって伸びている中空部、および当該開口部の反対側で当該中空部を閉じている底部を有している。つまり、当該窪みは、生体試料収容容器を収容し得る形状である。柔軟な材料によって作製されている袋に窪みを設けることは、極めて容易である。したがって、具体例1の袋及び具体例2の袋は、窪みの有無を除いて、同じ利点を有している。
図3の1030は、加温容器20に生体試料収容容器21を収容する前の状態を示しており、図3の1031は、加温容器20に生体試料収容容器21を収容した後の状態を示している。図3の1030に示すように、熱媒体収容容器23には熱媒体25が収容されており、生体試料収容容器21には生体試料24が収容されている。生体試料収容容器21の開口部上には、生体試料収容容器21の熱媒体収容容器23内での位置を固定する固定部22が設けられている。
図4の1040は、加温容器30に生体試料収容容器31を格納する前の状態を示しており、図4の1041は、加温容器30に生体試料収容容器31を格納した後の状態を示している。図4の1040に示すように、熱媒体収容容器33には熱媒体35が収容されており、生体試料収容容器31には生体試料34が収容されている。加温容器30は、ドーナツ状の固定部36を備えた点において加温容器20と異なっている。
図5の1050は、加温容器40に生体試料収容容器41を格納する前の状態を示しており、図5の1051は、加温容器40に生体試料収容容器41を格納した後の状態を示している。図5の1050に示すように、熱媒体収容容器43には熱媒体45が収容されており、生体試料収容容器41には生体試料44が収容されて蓋42におり封止されている。加温容器40は、熱媒体収容容器43内に当接部材48を備えた点において加温容器20と異なっている。
図6の1060は、加温容器50に生体試料収容容器51を格納する前の状態を示しており、図6の1061は、加温容器50に生体試料収容容器51を格納した後の状態を示している。図6の1060に示すように、熱媒体収容容器53には熱媒体55が収容されており、生体試料収容容器51には生体試料54が収容されている。加温容器50は、カバー52を備えた点において加温容器20と異なっている。
図7の1070は、加温容器60に生体試料収容容器61を格納する前の状態を示しており、図7の1071は、加温容器60に生体試料収容容器61を格納した後の状態を示している。図7の1070に示すように、熱媒体収容容器63には熱媒体65が収容されており、生体試料収容容器61には生体試料64が収容されている。加温容器60は、位置規定部68を備えた点において加温容器20と異なっている。
本発明の一態様に係る生体試料を加温するためのキットは、熱媒体及び生体試料収容容器を収容するための熱媒体収容容器を備えている。前記熱媒体収容容器は、前記生体試料収容容器の位置を規定するための位置規定部を有しており、前記生体試料収容容器は生体試料を収容するためのものである。
本発明の好ましい一態様に係る生体試料を加温するためのキットは、熱媒体を収容するための加温容器と、生体試料を収容するためのものであり、前記加温容器に収容可能な生体試料収容容器と、を備えている。生体試料を加温するためのキットは、さらに、生体試料を洗浄するための洗浄液を備えていてもよく、また、生体試料を加温するためのキットは、洗浄した生体試料を保管する保管液を備えていてもよい。
本発明の一態様に係る加温方法における加温条件を模擬的な環境において評価した。説明の便宜上、本発明の加温方法を「Tube in Tube法」と称する。模擬的な環境として、細胞ではなく、1mLの凍結保存用溶液を凍結した凍結試料を生体試料として用いて、加温条件を評価した。凍結保存用溶液の組成は、90%(v/v)STK(登録商標)2(サイトカインフリー)+10%(v/v)DMSO(Wako 031-24051)である。加温容器として、位置規定部を有さない遠沈管を用いた。1mLの凍結保存用溶液を生体試料収容容器としての凍結ベッセル(クライオジェニックバイアル:WHEATON,Cat.W985865)中に収容し、-80℃のディープフリーザー下で凍結した。凍結ベッセルを、表1に示す温度及び量の熱媒体が収容された遠沈管(50mL 遠沈管:住友ベークライト株式会社Cat.MS-56501)に格納し、それぞれ表1に示す方法で熱媒体を循環させ、凍結ベッセル内の生体試料を加温して解凍した。
位置規定部を有する加温容器を用いて、本発明の一態様に係る加温方法を模擬的な環境において評価した。まず、位置規定部を有する加温容器を以下に示すように準備した。図10に熱媒体収容容器及び生体試料収容容器の一例を示す。図10に示すように、熱媒体収容容器となるチューブ91と、位置規定部を構成するための樹脂膜93と、蓋94と、生体試料収容容器として凍結ベッセル92を用いた。なお、チューブ91として、50mLの遠沈管を用いた。
in Tube法により生体試料を加温して解凍したところ、解凍時間にバラつきが生じた(170秒~220秒)。この解凍時間は、表1の条件4の解凍時間の1.1倍~1.4倍に相当し、解凍時間が長くなった。また、全体で1分間程度のバラつきがあること自体も問題であった。このように、解凍特性の再現性に課題があることが分かった。そこで、さらに検討を重ねた結果、樹脂膜93と凍結ベッセル92との間に空気層がある場合や、樹脂膜93にたるみがある場合に解凍時間が長くなることが分かった。
前十字靭帯再建術などで余剰となった滑膜組織が、適切な倫理審査を経て患者同意の下に、医療機関から提供された。提供された滑膜組織の湿重量を測定し、ゲンタマイシン(日医工)含有DMEM(SIGMA) 10mLが入った遠沈管に移し、洗浄した。さらに、新たなゲンタマイシン含有DMEM 10mLが入った遠沈管に移しもう一度、洗浄した後、容器上に取り出した。洗浄後の滑膜組織は滅菌済ハサミを使用して5mm以下の組織片となるよう、容器上で細切し、さらゲンタマイシン含有DMEMで懸濁し、続いて滑膜組織片を50mL遠沈管中に回収した。その後、室温、1500rpm、5分間、遠心分離を行い、上清を除去した。
増殖したMSCをPBS(Phosphate buffered saline、カルシウム及びマグネシウムフリー、PBS(-)、株式会社細胞科学研究所)で1回洗浄した後、細胞剥離剤TrypLE Select CTS(Thermo Fisher
Scientific Inc.)で剥離、回収して洗浄培地(DMEM, Sigma)にて懸濁した後、遠心分離用のチューブに移し、室温で5分間、1500rpmにて遠心し、ペレットダウンした後に上清を除去した。
増殖したMSC(3継代目:P3)をPBS(-)で1回洗浄した後、細胞剥離剤TrypLE Select CTSで細胞をディッシュから剥離、回収してDMEMにて懸濁した後、遠心分離用のチューブに移し、室温で5分間1500rpmにて遠心分離し、ペレットダウンした。ペレットダウンされたシングルセル状態の細胞を再び洗浄培地にて懸濁し、トリパンブルー染色により細胞数をカウントした。
MSC(5継代目:P5)をPBS(-)で1回洗浄した後、細胞剥離剤TrypLE Select CTSで剥離、回収してDMEMにて懸濁した後、遠心分離用のチューブに移す。室温で5分間、1500rpmで遠心分離し、ペレットダウンし上清を除去した細胞をDMEMにて懸濁する。再び室温で5分間、1500rpmで遠心分離し、ペレットダウンし、上清を除去した細胞をDMEMにて懸濁した後、セルストレイナーを通し細胞を均一化する。さらに室温で5分間、1500rpmで遠心分離し、ペレットダウンし、上清を除去した細胞をSTK(登録商標)2により懸濁し、トリパンブルー染色により細胞数をカウントした。
登録商標)1の細胞塊を得た。
6well plate gMSC(登録商標)1をPBS(-)で2回洗浄した後、280U/ml コラゲナーゼ/50% TrypLE select溶液 1mL入った15mL遠沈管に移し、37℃で消化を行った。10分毎に10回転倒混和を行い、完全に塊が消えるまで消化し、トリパンブルー染色により総細胞数と生細胞数を測定した。280U/ml コラゲナーゼ/50% TrypLE select溶液の組成は以下の通り:コラゲナーゼ:Worthington biochemical corporation, Cat. LS004154 Lot :44D14883,TrypLE select: Thermo Fisher Scientific Inc., Cat.A12859-01 Lot:1905779,DMEM:Sigma, Cat. D6046, Lot: RNBG0276。
6well plate gMSC(登録商標)1をPBS(-)で2回洗浄した後、凍結保存用溶液1mLの入った2mL クライオバイアルに移し、-80℃で自然凍結を行った。凍結保存用溶液の組成は、90%(v/v)STK(登録商標)2(サイトカインフリー)+10%(v/v)DMSO(Wako 031-24051)である。
-80℃で1週間保存した後、フリーザーから凍結バイアルを取り出し、後述する加温実験に供した。加温による解凍後のgMSC(登録商標)1をDMEMにより洗浄し、280U/ml コラゲナーゼ/50% TrypLE select溶液により消化を行い、トリパンブルー染色により総細胞数と生細胞数を測定した。
上記2-5でシングルセル化されたgMSC(登録商標)1をDMEMで2回洗浄後、6well plateに5,000cells/cm2で再播種を行い、5日目の総細胞数及び生細胞数を測定した。
Tube in Tube法(非接触ではない)の効果を評価するために、同一方法で作製・凍結保存したgMSC(登録商標)1において、加温方法による細胞性能を比較した。本実施例においては加温方法として、「Tube in Tube法(以下の「解凍法B」参照)、37℃ウォーターバス法(以下の「解凍法A」参照)、及び37℃ヒートブロック法(以下の「解凍法C」参照)による解凍を行った。なお、37℃ヒートブロック法とは、ストレックス社製のヒートブロック装置(型番SY-1)を用い、37℃に設定して解凍する方法である。なお、gMSC(登録商標)1は3次元細胞塊であるため、細胞数を測定するためには上記2-5に記載のように細胞塊をシングルセルサスペンジョンにまで消化・分解する必要がある。このため、「凍結前の状態の細胞」を意味する対照群として、凍結操作を行わずに直ちに上記2-5に従って細胞数をカウントした群(以下の「非凍結群」参照)を別途用意した。実験の詳細を以下に示す。
上記2-1に基づいて樹立した3株(株が異なればドナーが異なる)のMSC(株1、株2、株3)を、上記2-2~2-4の方法に従ってgMSC(登録商標)1に加工した。各株由来のgMSC(登録商標)1を、それぞれ、非凍結群、解凍法A群、解凍法B群、解凍法C群の4群(したがって3株×4群の合計12群が存在する)に群わけした。それぞれの株において、サンプルサイズは、非凍結群(N=3)、解凍法A群(N=3)、解凍法B群(N=3)、解凍法C群(N=3)であった。
解凍法A~C群のgMSC(登録商標)1は、いずれも上記2-6に従って凍結保存した後、各群について以下の加温方法によって解凍した:
・解凍法A群:37℃のウォーターバスを用いて2.5分間解凍
・解凍法B群:Tube in Tube法により、22℃(室温)、約60rpmで3分間転倒混和を行い解凍
・解凍法C群:37℃に設定したヒートブロックで4.5分間解凍。
株1における解凍結果を図16に示し、株2における解凍結果を図17に示し、株3における解凍結果を図18に示した。図16~18に示す棒グラフの項目軸は、左から「非凍結群」、「解凍法A群」、「解凍法B群」、「解凍法C群」に対応している。また、それぞれの項目において、左側の白いバーは「gMSC(登録商標)1の一個(1drop)当たりの総細胞数」を示し、右側の黒いバーは「gMSC(登録商標)1の一個当たりの生細胞数」を示している。また、それぞれのバーにおけるエラーバーは、標準偏差を表している。
一般に、凍結・解凍直後の細胞は一時的に増殖能が低下することが知られている。そこで、上記の凍結・解凍を経たgMSC(登録商標)1から回収した細胞において、解凍直後の1継代の増殖能(立ち上がり)を比較した。具体的には、上記の凍結・解凍及び2-5に従ってシングルセルサスペンジョンにまで消化・分解した上記解凍法A~C群のそれぞれの細胞を、6wellプレート上に再播種し、一定期間単層培養した後の総細胞数及び生細胞数を比較した。
非接触Tube in Tube法の効果を評価するために、同一方法で作製・凍結保存したgMSC(登録商標)1に対する加温方法による細胞性能を比較した。本実施例においては、加温方法として、「非接触Tube in Tube法(以下の「解凍法D」参照)、37℃ウォーターバス法(以下の「解凍法A」参照)よる解凍をおこなった。
上記2-1に基づいて樹立した1株のMSCを、上記3-1と同様に、上記2-2~2-4の方法に従ってgMSC(登録商標)1に加工した。これらのgMSC(登録商標)1を、それぞれ、非凍結群、解凍法A群、解凍法D群の3群に群わけした。サンプルサイズは、非凍結群(N=3)、解凍法A群(N=3)、解凍法D群(N=3)であった。
解凍法A又はD群に割り当てたgMSC(登録商標)1は、いずれも上記2-6に従って凍結した後、各群について以下の加温方法によって解凍した:
・解凍法A群:37℃のウォーターバスを用いて2.5分間解凍
・解凍法D群:非接触Tube in Tube法により、22℃(室温)、約60rpmで3分間転倒混和を行い解凍。
図22は解凍後の総細胞数及び生細胞数を示している。図22の凡例は、図16~21と概ね同様であるが、プロットエリア中の二本の横線のうち、実線は解凍法D群の総細胞数(平均値)を示し、点線は解凍法D群の生細胞数(平均値)を示している。図22に示した結果から、非接触Tube in Tube法(解凍法D群)で解凍した場合、37℃ウォーターバス法(解凍法A群)と比較して、総細胞数及び生細胞数のいずれも同等、あるいはそれ以上であることが分かった。
一般に、凍結・解凍直後の細胞は一時的に増殖能が低下することが知られている。そこで、上記の凍結・解凍を経たgMSC(登録商標)1から回収した細胞を1継代培養した際の増殖能(立ち上がり)を比較した。具体的には、上記の凍結・解凍及び2-5に従ってシングルセルサスペンジョンにまで消化・分解した上記解凍法A群及びD群の細胞それぞれを、6wellプレート上に再播種し、一定期間単層培養した後の総細胞数及び生細胞数を比較した。
この項目では、gMSC(登録商標)1の代わりに、均質な細胞集団(ヒト皮膚線維芽細胞及びヒト脂肪由来MSC)を用いて、解凍法Dの有効性をさらに評価した結果を説明する。
適切な倫理審査を経て患者同意の下に、関係医療機関から提供された脂肪組織の湿重量を測定し、10μg/mLゲンタマイシン(日医工株式会社)を含有するDMEM(SIGMA、D6046)10mLが入った遠沈管に移し、洗浄した。さらに、新たなゲンタマイシン含有DMEM 10mLが入った遠沈管に移し、もう一度洗浄した後、容器上に取り出した。洗浄後の脂肪組織は滅菌済ハサミを使用して5mm以下の組織片となるよう、細切し、0.4%コラゲナーゼ溶液(Worthington Biochemical Corporation)により37℃で1.5時間、消化を行った後、100μmメッシュ(Greiner Bio-One International GmbH)でろ過し、新たな50mL遠沈管に回収した。遠心分離し、上清を除去し、細胞をSTK(登録商標)1(初代MSC樹立用無血清培地、株式会社DSファーマバイオメディカル)に懸濁した。細胞懸濁液の一部を0.4%トリパンブルー(Thermo Fisher Scientific Inc.)で染色し、生細胞数及び死細胞数をカウントした。5000cells/cm2(培養皿表面積)の播種密度に細胞懸濁液をSTK(登録商標)1で希釈し、150cm2dish(住友ベークライト株式会社)上に播種し、CO2濃度5%、37℃で14日間培養を行った(5日目、8日目、11日目に培地交換を実施した)。項目2-2および2-3の記載と同様の操作にてヒト脂肪由来MSC(A31、P4、以下「ADMSC」と記載する)を培養した。
前述のADMSCはSTK(登録商標)2培地で、株式会社ロンザジャパンから入手した、ヒト皮膚線維芽細胞(NHDF、P14、以下「NHDF」と記載する)は10%FBSを含むDMEM培地で、それぞれCO2濃度5%、37℃で培養した。回収(剥離及び洗浄)後の細胞懸濁液を、1mLの細胞保存用溶液を入れた2mLクライオバイアルに移した。ADMSC用の細胞保存用溶液は、CELLBANKER2(日本全薬工業株式会社)であり、NHDF用の細胞保存用溶液は、CellBanker1(日本全薬工業株式会社)である。各細胞懸濁液を-80℃で凍結させ、凍結から1週間後に、解凍法Aによって解凍した細胞集団である解凍法A群(N=3)及び解凍法Dによって解凍した細胞集団である解凍法D群(N=3)を得た。「解凍法A」及び「解凍法D」の詳細は、項目3-1に記載の通りである。
図24は、解凍後の総細胞数及び生細胞数を、NHDF(上パネル)及びADMSC(下パネル)のそれぞれについて示している。図24に示す通り、解凍法D群は、解凍法A群と比べて、総細胞数及び生細胞数の低下をほとんど示さなかった。
分取した残りの各細胞懸濁液に含まれている細胞の増殖能を評価した。各細胞懸濁液にDMEMを加え、細胞を洗浄した後に、細胞を遠心分離した。遠心分離されたNHDF(解凍法A群及び解凍法D群の両方)は、10%FBSを含むDMEM培地で再懸濁した。遠心分離されたADMSC(解凍法A群及び解凍法D群の両方)は、STK(登録商標)2培地で再懸濁した。解凍法A群及び解凍法D群の再懸濁したNHDF及びADMSCを、5×104細胞/ウェルで6ウェルプレートに播種した。NHDFを播種した6ウェルプレートを、5%CO2、37℃の培養条件に5日間おいた。ADMSCを播種した6ウェルプレートを、5%CO2、37℃の培養条件に7日間おいた。
2 熱媒体収容容器
3 開口部(入れ口)
4 熱媒体
5 生体試料収容容器
11 樹脂膜(位置規定部)
Claims (18)
- 熱媒体が収容された熱媒体収容容器に、生体試料が収容された生体試料収容容器を収容する収容工程と、
前記熱媒体が前記熱媒体収容容器の外に漏れないように前記熱媒体収容容器の前記熱媒体の入れ口を閉鎖する閉鎖工程と、
前記熱媒体の入れ口が閉鎖した前記熱媒体収容容器内の前記熱媒体を運動させる運動工程と、
を含む生体試料の加温方法。 - 前記閉鎖工程が、前記収容工程後に実施され、前記運動工程は、前記熱媒体収容容器を運動させることによって実施される、請求項1に記載の生体試料の加温方法。
- 前記運動工程は、前記熱媒体収容容器を手で把持して振ることにより行う、請求項1又は2に記載の生体試料の加温方法。
- 前記運動工程を、前記熱媒体が20℃以上、40℃以下の状態で行う、請求項1から3のいずれか1項に記載の生体試料の加温方法。
- 前記運動工程を、前記熱媒体が20℃以上、27℃以下の状態で行う、請求項4に記載の生体試料の加温方法。
- 前記生体試料が、細胞、細胞塊、組織及び組織片からなる群より選ばれる少なくとも一つである、請求項1から5のいずれか1項に記載の生体試料の加温方法。
- 前記熱媒体が、水、等張液、及び抗菌剤を溶解した水から成る群より選ばれる少なくとも1種である、請求項1から6のいずれか1項に記載の生体試料の加温方法。
- 生体試料が収容される生体試料収容容器を収容し、かつ熱媒体が内部に収容される、熱媒体収容容器と、
当該熱媒体収容容器に設けられた、前記生体試料収容容器の位置を規定するための位置規定部とを備えた、生体試料の加温容器。 - 前記位置規定部は、当該熱媒体収容容器内に設けられている、請求項8に記載の生体試料の加温容器。
- 当該熱媒体収容容器は袋状であり、前記位置規定部は、当該熱媒体収容容器の外表面であり、袋状の前記熱媒体収容容器を折りたたんで前記生体試料収容容器を挟むことで、生体試料収容容器を収容し、かつ、位置を規定するものである、請求項8に記載の生体試料の加温容器。
- 前記位置規定部が樹脂膜である、請求項8に記載の生体試料の加温容器。
- 前記熱媒体収容容器は、一端が閉鎖され、他端が開口している筒状構造であり、開口している当該他端を閉鎖するための蓋をさらに備えた、請求項8又は11に記載の生体試料の加温容器。
- 前記筒状構造の長さ方向に垂直な断面の直径が5mm以上、200mm以下である、請求項12に記載の生体試料の加温容器。
- 内部に発熱体を有さない、請求項8から13のいずれか1項に記載の生体試料の加温容器。
- 熱媒体及び生体試料収容容器を収容するための熱媒体収容容器を備え、
前記熱媒体収容容器は、前記生体試料収容容器の位置を規定するための位置規定部を有しており、前記生体試料収容容器は生体試料を収容するためのものである、
生体試料を加温するためのキット。 - 前記生体試料収容容器には、低温保存された生体試料が予め収容されており、
前記生体試料の低温状態を維持するための保冷手段をさらに備えた、請求項15に記載の生体試料を加温するためのキット。 - 生体試料を洗浄するための洗浄液をさらに備えた、請求項15又は16に記載の生体試料を加温するためのキット。
- 前記生体試料収容容器には、生体試料が予め収容されており、
前記生体試料収容容器は、密封された容器内に滅菌されて収容されている、請求項15から17のいずれか1項に記載の生体試料を加温するためのキット。
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