WO2015192328A1 - Appareil d'isolation d'extrait et son procédé de fonctionnement - Google Patents

Appareil d'isolation d'extrait et son procédé de fonctionnement Download PDF

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Publication number
WO2015192328A1
WO2015192328A1 PCT/CN2014/080126 CN2014080126W WO2015192328A1 WO 2015192328 A1 WO2015192328 A1 WO 2015192328A1 CN 2014080126 W CN2014080126 W CN 2014080126W WO 2015192328 A1 WO2015192328 A1 WO 2015192328A1
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WO
WIPO (PCT)
Prior art keywords
liquid
adsorption
separation
reaction
disc
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PCT/CN2014/080126
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English (en)
Chinese (zh)
Inventor
王海
王光亮
翁彦雯
黄修涛
Original Assignee
深圳迈瑞生物医疗电子股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 深圳迈瑞生物医疗电子股份有限公司 filed Critical 深圳迈瑞生物医疗电子股份有限公司
Priority to PCT/CN2014/080126 priority Critical patent/WO2015192328A1/fr
Priority to CN201910679522.1A priority patent/CN110488029B/zh
Priority to CN201480074414.7A priority patent/CN105940305B/zh
Publication of WO2015192328A1 publication Critical patent/WO2015192328A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations

Definitions

  • the present application relates to the field of purification or concentration technology, and in particular to an extract separation device and a method of its operation.
  • the extract separation method has a wide range of applications in the field of purifying or concentrating biological substances.
  • the magnetic separation technique is usually used to extract the components of interest from the solution.
  • the basic principle is to use a small particle surface with a magnetic material to pass a certain treatment. It can adsorb the required substances, then discard the waste liquid after adsorption and enrichment by magnets, and clean the enriched magnetic beads to further remove impurities and other unwanted substances, and the biological material of interest can be adsorbed on the magnetic beads. Retaining, finally, the biological material enriched in the magnetic beads is released into the desired solution system under certain conditions.
  • Figure 1 shows a basic magnetic separation process. The steps listed are:
  • Step 1 The beads are combined.
  • the magnetic bead solution is added to the solution to be purified, and the component of interest which needs to be purified or concentrated is adsorbed onto the surface of the magnetic bead.
  • the strips are the components of interest, the blocks are impurities, and the spheres are magnetic beads.
  • Step 2 Magnetic beads adsorption and cleaning separation.
  • the magnetic beads are gathered together by an external magnetic field, thereby facilitating cleaning and removing impurities;
  • Step 3 Elution release, the cleaned magnetic beads are re-added to the desired solution to release the interesting components from the magnetic beads, or directly for subsequent testing.
  • the magnetic separation device is mainly used to complete step 2, and generally includes a liquid injection mechanism for injecting a cleaning liquid into a reaction container containing a solution for completing magnetic bead bonding, a liquid absorption mechanism for drawing waste liquid from the reaction container, and magnetic properties.
  • the mechanism and the moving mechanism are for moving the reaction container between the liquid injection mechanism and the liquid suction mechanism.
  • the reaction vessel can be removed after it has been cleaned and separated.
  • the commonly used magnetic separation device comprises a plurality of liquid absorption positions and a plurality of liquid injection positions, a magnetic field is set at the liquid absorption position, and there is no magnetic field at the liquid injection position, and each liquid absorption position is placed in a reaction container containing the reaction solution.
  • the plurality of liquid suction mechanisms respectively perform parallel liquid suction operations on the plurality of reaction containers, and after the liquid absorption is completed, the reaction containers are moved to the corresponding liquid injection positions, and then the plurality of liquid injection mechanisms respectively perform parallel operation on the plurality of reaction containers. The injection operation. Summary of the invention
  • an extract separation device comprising:
  • the separating disc is a rotatable ring mechanism, and the separating disc is provided with a plurality of reaction cups arranged in at least one row for carrying a reaction container along an annular space, the separating disc Having at least one liquid absorption position and at least one liquid injection position along the rotation path thereof;
  • the adsorption mechanism, the adsorption mechanism is a ring mechanism, and the adsorption mechanism is provided with one or more adsorbable extract binding carriers along the ring shape a component, and at least one vacancy position on the adsorption mechanism that does not adsorb the '1'component;
  • a liquid filling mechanism for performing a liquid filling operation at a liquid filling position
  • the moving mechanism for driving the separation disc and the suction mechanism can be independently rotated and stopped, and the vacancy of the moving mechanism driving the adsorption mechanism follows the reaction cup movement without the adsorption force.
  • an extract separating apparatus comprising:
  • the separating disc is a rotatable ring mechanism, and the separating disc is provided with a plurality of reaction cups arranged in at least one row for carrying the reaction container along the annular interval, and the separating disc is distributed along the rotation path thereof Having at least one pipetting position and at least one liquid filling position;
  • the adsorption mechanism is an annular mechanism, and the adsorption mechanism is provided with one or more adsorption components capable of adsorbing the extract binding carrier along the ring shape, and at least one of the adsorption mechanisms is not adsorbed. Vacancy position of the part;
  • a liquid filling mechanism for performing a liquid filling operation at a liquid filling position
  • the movement mechanism drives the vacancy of the adsorption mechanism to follow the reaction cup of the reaction vessel Bit synchronization moves.
  • a method for magnetic separation using a magnetic separation device comprising an annular separation disk for carrying a reaction vessel and an annular adsorption mechanism
  • the separation disc is provided with a plurality of reaction cups arranged in at least one row for carrying the reaction container along the annular space, and the separation disc is distributed along the rotation path thereof with at least one liquid absorption position and at least one liquid injection position, the adsorption
  • the mechanism is provided with one or more adsorbing members capable of adsorbing the extract binding carrier along the ring shape, and at least one vacancy position without the adsorptive member is disposed on the adsorption mechanism; the method comprises: the separating disk drives the reaction container Rotating and stopping for operation, the vacancy position of the moving mechanism driving the adsorption mechanism moves with a position that does not need to provide an adsorption force to the extract binding carrier, and the opposite direction is stopped during the stop of the separation disk to drive the reaction container.
  • an extract separating apparatus comprising:
  • the separation disc is a rotatable ring mechanism, and the separation disc is spaced along the ring Having a plurality of reaction cups arranged in at least one row for carrying the reaction vessel, the separation disc having at least one liquid absorption position and at least one liquid injection position distributed along the rotation path thereof;
  • the adsorption mechanism is an annular mechanism, and the adsorption mechanism is provided with one or more adsorption components capable of adsorbing the extract binding carrier along the ring shape, and at least one of the adsorption mechanisms is not adsorbed. Vacancy position of the part;
  • the moving mechanism for driving the separation disc and the suction mechanism can be independently rotated and stopped.
  • an extract separating apparatus comprising:
  • the separating disc is a rotatable ring mechanism, and the separating disc is provided with a plurality of reaction cups arranged in at least one row for carrying the reaction container and a flow tube for carrying the waste liquid a flow tube position, the flow tube position being adjacent to the corresponding reaction cup position, the separation disc being distributed along the rotation path thereof with at least one liquid absorption position and at least one liquid injection position;
  • the adsorption mechanism is an annular mechanism, and the adsorption mechanism is provided with one or more adsorptive members that can adsorb the extract-binding carrier along the ring shape, and the position distribution of the adsorptive member is at least compared with the suction on the separation disk.
  • the liquid position corresponds to the position, and at least one vacancy position without the adsorption component is disposed on the adsorption mechanism, the position distribution of the vacancy position corresponds to the liquid injection position; and the liquid suction mechanism for performing the liquid absorption operation at the liquid absorption position;
  • a liquid filling mechanism for performing a liquid filling operation at a liquid filling position
  • an extract separating apparatus comprising:
  • the separating disc is a rotatable ring mechanism, and the separating disc is provided with a plurality of reaction cups arranged in at least one row for carrying the reaction container and a flow tube for carrying the waste liquid a flow tube position, the flow tube position being adjacent to a corresponding reaction cup position, the separation disk being distributed along the rotation path thereof with at least one liquid absorption position, at least one liquid injection position and at least one mixed hook position, the note The liquid position and the mixed hook position are adjacent;
  • the adsorption mechanism is an annular mechanism, and the adsorption mechanism is provided with one or more adsorptive members that can adsorb the extract-binding carrier along the ring shape, and the position distribution of the adsorptive member is at least compared with the suction on the separation disk.
  • the liquid position corresponds to the position, and at least one vacancy position without the adsorption component is disposed on the adsorption mechanism, and the position distribution of the vacancy position corresponds to at least one of the liquid injection position and the mixing position;
  • a pipetting mechanism for performing a pipetting operation at the pipetting position
  • a liquid filling mechanism for performing a liquid filling operation at a liquid filling position
  • Figure 1 is a basic magnetic separation process
  • FIG. 2 is a schematic structural view of an extract separating device in the first embodiment of the present application
  • FIG. 3 is a flow chart of extract separation in a working cycle in the first embodiment of the present application
  • FIG. 4 is a schematic structural view of a flow pipe in the second embodiment of the present application
  • FIG. 5 is a schematic view showing the separation of the extract in one working cycle in the second embodiment of the present application; and FIG. 6 is a schematic structural view of the extract separating device in the third embodiment of the present application.
  • the embodiment of the present application is intended to provide an extract separating device for performing a flow operation of a single reaction vessel as a processing unit, wherein at least one column of reaction cups for carrying a reaction vessel is distributed on the extract separating device, along the The rotation path is distributed with a plurality of liquid absorption positions and at least one liquid injection position.
  • a predetermined operation corresponding to the operation position is performed to the reaction container stopped at the liquid absorption position or the liquid injection position.
  • different operations have different requirements for the adsorption capacity of the extract-binding carrier. Some operations (or time periods) require adsorption, while others do not require adsorption. Have. Therefore, during the rotation or stop of the reaction vessel, the vacancy position at which the moving mechanism drives the adsorption mechanism without the adsorption force moves without providing an adsorption force position to the extract-binding carrier.
  • the extract separating apparatus 10 includes an adsorption mechanism 1 1 , a separation disc 12 , a liquid suction mechanism 13 , a liquid injection mechanism 14 , and a moving mechanism 15 .
  • the separating disc 12 is a ring mechanism, and the ring mechanism can be cyclically rotated, and the rotating path can be circular, elliptical, various polygonal or irregular closed shapes, and the separating disc is provided with a plurality of ring-shaped intervals for carrying the reaction.
  • the reaction cup position 121 of the container 1 22 and the plurality of reaction cup positions 1 21 are arranged in at least one column.
  • the separation disc 12 is also distributed along its rotational path with at least one liquid-absorbent position and at least one liquid-filling position.
  • the so-called liquid suction position is a position at which the reaction container 122 which is aspirating operation is stopped when the separation disk is stopped for operation, and the liquid suction mechanism 13 performs a liquid suction operation on the reaction container stopped at the liquid absorption position.
  • the so-called liquid filling position is a position at which the reaction container 122 in which the liquid filling operation is stopped when the separation disk is stopped for operation, and the liquid filling mechanism 14 performs a liquid filling operation on the reaction container stopped at the liquid filling position.
  • the liquid absorption position and the liquid injection position may each have one or more. In a specific embodiment, at least part of the liquid absorption position and the liquid injection position are alternately set, and the interval between the liquid absorption position and the liquid injection position is thousands of reaction cup positions.
  • the liquid filling position is set after a plurality of reaction cup positions 121 are separated from each other, and the liquid filling position is set after a plurality of reaction cup positions 121.
  • the number of pipetting positions is greater than the number of liquid filling positions, so that in the direction of rotation of the separation disk 12, the liquid suction position and the liquid filling position are alternately set first, and then the remaining liquid suction positions are set. In order to absorb the waste liquid more thoroughly after washing.
  • the amount of the aspiration position and the position of the infusion can be set according to the specific needs of the extract.
  • a plurality of reaction cup positions 121 are arranged in a row.
  • a plurality of reaction cup positions 121 may be arranged in two or three columns as needed, and each column may have a respective suction.
  • the liquid position and the injection position can also share the aspiration position and/or the injection position.
  • the separating disc 12 is further provided with a plurality of rings for carrying the load.
  • the flow tube position 123 of the flow tube of the waste liquid is adjacent to the corresponding reaction cup position of the flow tube position 123.
  • the flow tube is used to hold the waste liquid aspirating mechanism 1 3 The waste liquid sucked from the reaction container corresponding to the flow tube.
  • the pipetting mechanism 13 is for performing a pipetting operation at the pipetting position.
  • the pipetting mechanism 13 is located above the pipetting position, and the pipetting operation is performed by the up and down movement; in other embodiments, the pipetting The mechanism 13 may not be located above the liquid suction position but in the vicinity of the liquid suction position, the liquid suction head is moved to the upper position of the liquid suction position by rotation, and then the liquid suction operation is performed by the up and down movement.
  • the number of the liquid suction mechanisms 13 is the same as the number of liquid suction positions, and each of the liquid suction mechanisms 13 has its corresponding liquid suction position.
  • the function of the aspiration operation is to take the waste liquid from the reaction vessel, leaving the carrier-bound extract and the binding carrier.
  • the waste liquid may be the waste liquid generated after the addition of the reagent, or may be the waste liquid generated after the addition of the cleaning liquid. .
  • the liquid injection mechanism 14 is configured to perform a liquid injection operation at the liquid injection position.
  • the liquid injection mechanism 14 is located above the liquid injection position, and the liquid injection operation is performed by the up and down movement; in other embodiments, the liquid injection mechanism 14 Instead of being located above the liquid injection position, in the vicinity of the liquid injection position, the liquid injection head is moved to the upper position of the liquid injection position by rotation, and then the liquid injection operation is performed by the up and down movement.
  • the number of the liquid filling mechanisms 13 is the same as the number of the liquid filling positions, and each of the liquid filling mechanisms 14 has its corresponding liquid filling position.
  • the function of the liquid injection operation is to inject a cleaning liquid into the reaction vessel to wash the impurities adsorbed on the reaction vessel wall or the binding carrier into the solution.
  • the adsorption mechanism 11 is also a ring mechanism, and the adsorption mechanism 11 is provided with a plurality of adsorption members 111 that can adsorb the extract-binding carrier along the ring shape, and at least one of the adsorption mechanisms 11 is provided.
  • Vacancy bit 112 of the adsorbent component corresponds to the reaction cup position 121 on the separation disc 12, and the vacancy position 112 vacates at least one of the reaction cup positions without an adsorbing member.
  • the extract-binding carrier is a magnetic carrier, such as a magnetic bead
  • the adsorptive member 111 is a magnetic member such as a magnet.
  • the separation disc 12 and the adsorption mechanism 11 are annular mechanisms in which the inner and outer phases are nested.
  • the adsorption mechanism 11 may be on the outer side of the separation disc 12 or on the inner side of the separation disc 12, and has magnetic components.
  • the position corresponds to a magnetic field on the reaction cup position on the separation disc 12, and if there is a magnetic carrier adsorbed with the extract in the reaction vessel on the reaction cup position, the magnetic carrier is subjected to the magnetic force of the magnetic member, moving and adhering to the reaction
  • the container is adjacent to one side of the magnetic member; the position of the vacancy 112 having no magnetic member on the adsorption mechanism 11 corresponds to the absence of a magnetic field on the reaction cup position on the separation tray 12, and the reaction container on the reaction cup has an adsorbed extract even
  • the magnetic carrier is also not attracted by the magnetic force of the magnetic member to the side of the reaction container adjacent to the adsorption mechanism 11.
  • the adsorption mechanism 11 is located at the bottom of the separation disc 12, and when the position having the magnetic member corresponds to the reaction cup position on the separation disc 12, the magnetic carrier is subjected to the magnetic force of the magnetic member, moving and adhering to the reaction container Near the bottom of the magnetic part.
  • the vacancy 112 on the adsorption mechanism 11 may be provided only one, and the adsorption member 111 may have only one annular adsorption member; when the separation disk 12 rotates
  • the separation disk 12 rotates
  • the vacancy 112 on the adsorption mechanism 11 can also be A plurality of correspondingly arranged, and preferably the number of spaced cups between adjacent vacancies 112 is equal to the number of spaced cups between the injection locations. In order to allow the plurality of vacancies 112 to simultaneously follow a plurality of reaction cup positions that do not require adsorption.
  • the moving mechanism 15 is used to drive the separation disc 12 and the adsorption mechanism can be independently rotated and stopped.
  • the moving mechanism 15 drives the vacancy 112 of the adsorption mechanism 11 to follow the reaction vessel.
  • the reaction cup moves.
  • different operations have different requirements on the adsorption capacity of the extract-binding carrier, and sometimes an adsorption force is required, but sometimes no adsorption force is required, so the moving mechanism 15 drives the vacancy 112 of the adsorption mechanism 11 to follow the adsorption-free force.
  • the reaction cup position where the reaction vessel is located moves synchronously, so that the extract-binding carrier in the reaction vessel is not subjected to the adsorption force during this period, and the extract-binding carrier is suspended in the solution, which is advantageous for combining more extracts and cleaning.
  • the extract binds impurities on the carrier.
  • the vacancy of the moving mechanism driving the adsorption mechanism follows the reaction cup position of the reaction vessel to move synchronously. , thereby making the mention in the reaction vessel
  • the material-binding carrier is not subjected to adsorption force during the mixing stage after the addition of the cleaning solution.
  • the moving mechanism 15 Before the moving mechanism 15 drives the vacancy 1 1 2 of the adsorption mechanism 11 to follow the reaction cup of the reaction vessel to start synchronous movement, it is possible that the vacancy 1 12 of the adsorption mechanism 1 1 reacts with the unwanted adsorption force on the separation disc.
  • the moving mechanism 15 first drives the suction mechanism to rotate relative to the separation disc, so that the vacancy position is rotated to the position of the reaction container where the adsorption force is not required on the separation disc.
  • the mobile mechanism control process is as follows:
  • the moving mechanism drives the separation disc to stop for the liquid filling operation
  • the moving mechanism drives the adsorption mechanism to rotate relative to the separation disc, so that the vacancy position is rotated to the position corresponding to the liquid injection position, and during the injection from the reaction container to the mixing hook,
  • the vacancy of the moving mechanism driving the adsorption mechanism follows the reaction cup position of the reaction vessel to move synchronously.
  • the moving mechanism drives the adsorption mechanism to rotate relative to the separation disc, and transports the adsorption component to a position corresponding to the reaction cup position of the reaction container in which the end of the mixing operation is performed, and synchronizes with the reaction cup position. mobile.
  • the mixing operation is performed at the position of the mixing hook, and the position of the mixing hook is located on the rotation path of the separation disc, and the mixing position may be independent of the position of aspiration and liquid injection, or may not be located on the rotation path of the separation disc.
  • at least one of the liquid suction positions is used as a mixed hook position, and the liquid suction mechanism performs a mixed hook operation after the liquid injection by a continuous suction operation.
  • the separation method of the extract includes a sequential washing step and a separation step.
  • the cleaning steps include:
  • Step 30 a first pipetting step, the separating disk drives the reaction container to rotate, and when the separating disk stops for performing the liquid suction operation, the liquid suction mechanism performs a liquid suction operation on the reaction container stopped in the reaction cup position of the liquid suction position;
  • the adsorptive component of the adsorption mechanism corresponds to the reaction cup position in which the reaction vessel to be aspirated, provides an adsorption force for the extract-binding carrier in the reaction vessel, and the moving mechanism drives the adsorption mechanism and the separation The disc rotates and stops synchronously.
  • Step 31 The liquid filling step, when the separation tray drives the reaction container to stop for the liquid injection operation, the liquid injection mechanism performs a liquid injection operation on the reaction container stopped at the liquid injection position.
  • the moving mechanism drives the suction mechanism to rotate relative to the separation disc, so that the vacancy position is rotated to a position corresponding to the liquid injection position, and the liquid injection mechanism performs a liquid injection operation on the reaction container stopped at the liquid injection position.
  • Step 32 The step of mixing the hook, the moving mechanism drives the separation disc and the adsorption mechanism to rotate synchronously.
  • the separation tray drives the reaction container to stop for the mixed hook operation
  • the reaction container of the reaction cup position stopped at the mixed hook position is performed.
  • the moving mechanism drives the adsorption mechanism The rotation relative to the separation disc is caused to rotate the adsorption 'ti part to a position corresponding to the reaction cup position of the reaction vessel in which the mixing is completed.
  • Step 33 the separating step comprises a second pipetting step, the separating disk drives the reaction vessel to rotate, and the adsorption component of the adsorption mechanism corresponds to the reaction cup position of the reaction vessel to be aspirated, and provides the extract binding carrier in the reaction vessel.
  • the adsorption force, and the adsorption mechanism rotates synchronously with the separation disc.
  • the liquid suction mechanism performs a liquid suction operation for the separation purpose of the reaction container in the reaction cup position stopped at the liquid absorption position.
  • the adsorption mechanism does not rotate relative to the separation disc, and it is not necessary to consider whether the vacancy position is rotated to a position corresponding to the liquid injection position, and the adsorption mechanism is driven to be separated from the separation in the mixing step.
  • the rotation of the disc rotates the vacancy position to a position corresponding to the position of the mixed hook.
  • the moving mechanism drives the suction mechanism to perform the rotation relative to the separation disc again, so that the adsorption member is rotated to the reaction container with the completion of the hook.
  • the location corresponding to the reaction cup position Example 2:
  • the liquid suction mechanism In the process of separating the extract by the above-mentioned extract separating device, the liquid suction mechanism needs to operate different reaction containers. To avoid the common liquid suction head (also called the T ip head), the liquid suction mechanism adopts a disposable liquid suction head. This requires the liquid suction mechanism to constantly replace the new liquid suction head, resulting in waste of the liquid suction head.
  • the flow tube is designed as a structure capable of carrying a disposable liquid suction head, as shown in FIG. 4, the flow tube 40 includes at least two chambers.
  • a chamber 41 is provided for the disposable liquid suction head 42 of the reaction vessel corresponding to the flow tube, and the other chamber 43 is for holding the waste liquid.
  • the solution for aspirating the disposable liquid suction head during the magnetic separation of the extract is:
  • a separation disc 12 is used to carry a reaction vessel 122 that requires magnetic separation, and a flow tube 124 for use therewith.
  • the flow tube 124 functions to carry the liquid absorption Tip and store the waste liquid;
  • the liquid suction mechanism 13 and the liquid injection mechanism 14 disposed above the operation track of the reaction container, wherein the liquid absorption mechanism is arranged in total, and the liquid injection mechanism is arranged in a total of one.
  • the liquid suction mechanism needs to be loaded with T ip before the reaction cup can be sucked.
  • a liquid magnetic field ring is disposed on the outer side of the magnetic separation disk and is rotatable concentrically with the magnetic separation disk. The magnetic field ring is fixedly mounted with a magnet for adsorbing the magnetic beads in the reaction cup.
  • the magnetic separation discs are arranged in a total of 36 positions for carrying the reaction cup and the flow tube, respectively, wherein the reaction cup and the waste tube are separated into the magnetic separation disc.
  • the reaction cup and the flow tube were taken from the magnetic separation plate by the instrument.
  • the position is placed in the magnetic separation disk one after the other, and then as the magnetic separation disk rotates forward, in the process, the corresponding outer magnetic field ring position of the reaction cup has a magnetic field, so that the magnetic bead cup is adsorbed to the side of the reaction cup;
  • the liquid suction mechanism moves downward to load the Tip in the flow tube, and then lifts up;
  • the liquid suction mechanism descends to suck the liquid in the reaction cup and then lifts up; then the magnetic separation disc continues to advance to a position, so that the flow tube is located on the magnetic separation disc At position 9, then the aspiration mechanism moves downward to drain the waste and unload Ti p.
  • the magnetic separation disk since the position of the waste liquid in the flow pipe is inconsistent with the position of the unloading Ti p, the magnetic separation disk needs to be moved again to the position where the Tip is unloaded after the waste liquid is discharged, and of course, the design of the flow tube can be made The position is kept the same, and the step of moving the magnetic separation disc is not required. So far, the first-stage aspiration movement of the magnetic separation is completed.
  • the magnetic separation disk and the magnetic field ring of the outer ring thereof are kept in synchronization or stopped, so that the reaction cup is always in the magnetic field adsorption state.
  • the magnetic separation disc continues to advance to three positions, so that the reaction cup enters the 11th position on the magnetic separation disc, that is, below the liquid injection mechanism, and the magnetic field ring occurs during the advancement of the magnetic separation disc.
  • the relative motion makes the part of the magnetic field ring without the magnetic field corresponding to the reaction cup.
  • the liquid injection mechanism injects the cleaning liquid into the reaction cup without the magnetic field adsorption, and then the magnetic separation plate retreats back to the three positions so that the flow tube returns to the first In the first-stage aspiration position, the aspirating mechanism is lowered to load the Tip, and then the magnetic separation disc continues to retreat to a position, so that the reaction cup is located below the first-stage aspiration position, and the liquid suction mechanism descends to suck and discharge the cleaning liquid and the magnetic beads in the reaction cup.
  • the magnetic separation disc is further advanced to a position, so that the flow tube is located under the liquid suction mechanism to unload the Tip, and the first stage of the magnetic separation and the mixing action are completed, and the magnetic separation disk and the magnetic field ring are completed in the process.
  • the synchronous movement and stopping are maintained at all times, so the reaction cup is always in a state of no magnetic field adsorption, and the magnetic beads are resuspended in the cleaning liquid.
  • the magnetic field ring is re-rotated relative to the magnetic separation disc so that the reaction cup is in a magnetic field, and the magnetic field adsorption of the next step is continued.
  • the second-order magnetic separation aspiration and injection mixing operation is repeated with the above process, and then the third stage only performs the liquid absorption operation, and does not perform the liquid injection operation, sucking the liquid in the reaction cup, the fourth order is the same as the third
  • the steps are repeated, and only the liquid is absorbed by the liquid to absorb the residual liquid at the bottom of the cup. At this point, the entire magnetic separation operation is completed. Then the reaction cup and the flow tube continue to move back to position 36 with the magnetic separation disc, and the reaction cup and the flow tube are taken away by the instrument for subsequent operations.
  • the above process is designed for a specific extraction characteristic. According to the difference in the degree of cleanliness required for extraction and the difference in residual liquid, the number of times of aspirating and injecting can be increased or decreased, even in the end, the need for subsequent injection testing is increased.
  • the other reaction liquid components are all possible deformation schemes.
  • the separation disc can also be provided with only one liquid absorption position and one liquid injection position along its rotation path.
  • one working cycle is: first, the reaction container is transported to the liquid absorption position to perform the suction liquid waste. Operate, then transfer the reaction vessel to the fill level The injection liquid is injected into the reaction container, and finally the reaction container is transported to the liquid absorption position to perform the operation of sucking the waste liquid.
  • Example 3
  • the separation disc is a rotatable ring mechanism for driving the separation disc to rotate and stop.
  • the adsorption mechanism is a fixed non-rotating annular structure, and the adsorption mechanism is provided with a plurality of adsorption components capable of adsorbing the extract-binding carrier along the ring shape, and the position distribution of the adsorption component corresponds at least to the liquid absorption position on the separation disk.
  • the adsorption mechanism is provided with at least one vacancy position without an adsorption component, and the position distribution of the vacancy position corresponds to at least one of the liquid injection position and the mixing position, and the position distribution of the vacancy position and the injection position and the mixing position When they are all corresponding, the injection position and the mixing position are adjacent.
  • the separation process of the extract will be described below by taking magnetic separation as an example.
  • a rotatable magnetic separation disk 60 is provided with a plurality of reaction cups on the upper surface for carrying the reaction cup 61 requiring magnetic separation, and a magnetic field structure is arranged at a fixed position of the outer ring of the magnetic separation disk.
  • the magnetic member 62 is configured to adsorb magnetic beads in the reaction cup, and a liquid injection mechanism 63 and a liquid suction mechanism 64 that are movable up and down are disposed above the magnetic separation disk.
  • the magnetic field structure also includes a void location 65 having no magnetic components, and the void location 65 corresponds to the liquid injection location.
  • the reaction cup is placed in the magnetic separation disk, and the magnetic beads in the reaction cup are adsorbed to the side of the reaction cup by the magnetic field outside the magnetic separation disk, and then the reaction cup rotates forward with the magnetic separation disk, moving magnetic
  • the separation disc drives the reaction cup to continue moving forward, the reaction cup reaches below the liquid injection mechanism, and the liquid injection mechanism moves downward to inject the cleaning liquid into the reaction cup and mixes it. There is no magnetic field distribution outside the reaction cup below the liquid injection mechanism.
  • the magnetic beads are resuspended in the cleaning solution. After that, the magnetic separation disc drives the reaction cup to move forward to the next liquid suction mechanism.

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Abstract

L'invention porte sur un appareil d'isolation d'extrait et sur son procédé de fonctionnement ; l'appareil d'isolation d'extrait comprend un mécanisme d'adsorption (11), un disque de séparation (12), un mécanisme de pipetage (13), un mécanisme d'injection (14), et un mécanisme de mouvement (15) ; le disque de séparation est un mécanisme annulaire rotatif, et le long de ce dernier sont disposées une pluralité de positions de cuvette de réaction (121) espacées par intervalles ; le long de la trajectoire de rotation du disque de séparation sont réparties une pluralité de positions de pipetage et au moins une position d'injection ; le mécanisme d'adsorption est également un mécanisme annulaire ; le mécanisme d'adsorption comporte une pluralité de constituants adsorptifs (111) le long de sa périphérie, et ledit mécanisme d'adsorption comporte au moins une position vacante (112) n'ayant pas de constituant adsorptif ; le mécanisme de mouvement entraîne le disque de séparation et le mécanisme d'adsorption à tourner et à s'arrêter indépendamment, et la position vacante se déplace avec la position ne nécessitant pas de force adsorptive. Le disque de séparation et le mécanisme d'adsorption sont entraînés de façon à tourner et à s'arrêter indépendamment, de telle sorte que le mécanisme adsorptif peut fournir une force adsorptive ou ne pas fournir de force adsorptive, en fonction des exigences.
PCT/CN2014/080126 2014-06-17 2014-06-17 Appareil d'isolation d'extrait et son procédé de fonctionnement WO2015192328A1 (fr)

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