WO2014156379A1 - データ処理装置、光学検出システム、データ処理方法及びデータ処理プログラム - Google Patents
データ処理装置、光学検出システム、データ処理方法及びデータ処理プログラム Download PDFInfo
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- WO2014156379A1 WO2014156379A1 PCT/JP2014/053889 JP2014053889W WO2014156379A1 WO 2014156379 A1 WO2014156379 A1 WO 2014156379A1 JP 2014053889 W JP2014053889 W JP 2014053889W WO 2014156379 A1 WO2014156379 A1 WO 2014156379A1
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0162—Arrangements or apparatus for facilitating the optical investigation using microprocessors for control of a sequence of operations, e.g. test, powering, switching, processing
- G01N2021/0175—Arrangements or apparatus for facilitating the optical investigation using microprocessors for control of a sequence of operations, e.g. test, powering, switching, processing for selecting operating means
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N2021/1748—Comparative step being essential in the method
- G01N2021/1751—Constructive features therefore, e.g. using two measurement cells
- G01N2021/1753—Constructive features therefore, e.g. using two measurement cells and using two light sources
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N2021/178—Methods for obtaining spatial resolution of the property being measured
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N2021/1789—Time resolved
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- G—PHYSICS
- G01—MEASURING; TESTING
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6419—Excitation at two or more wavelengths
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
- G01N21/274—Calibration, base line adjustment, drift correction
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G—PHYSICS
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- G01N2201/00—Features of devices classified in G01N21/00
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N2201/04—Batch operation; multisample devices
- G01N2201/0484—Computer controlled
Definitions
- the present technology relates to a data processing device, an optical detection system, a data processing method, and a data processing program. More specifically, the present invention relates to a technique for specifying an analysis target range corresponding to a storage area in which a detection target is stored from light intensity distribution data.
- a device that optically detects a detection target In a device that optically detects a detection target, light is emitted from a light source toward an area in which the detection target is accommodated, and the detection target is detected by detecting light transmitted through the area or light emission from the detection target. Is done. Further, when it is desired to detect a plurality of detection targets included in a sample at once using the above-described optical detection apparatus, a plurality of lights having different wavelengths may be used in accordance with the optical characteristics of each detection target.
- a sample including a detection target is often stored in a container suitable for optical detection.
- a microplate or a microchip in which a plurality of holes called wells are formed has been conventionally used as a container for storing a sample.
- a plurality of regions for accommodating a sample are provided, such as a microplate and a microchip, in order to optically detect the detection target, light from the light source is irradiated to each region. There is a need.
- a plurality of optical systems such as a light source may be provided in the optical detection device.
- the optical detection device may be configured such that either the optical system such as the light source or the region in which the sample is accommodated moves and the relative position of the region with respect to the optical system can be changed.
- a light source or the like can cross between the regions, and light from the light source can be irradiated to a plurality of regions in order.
- Patent Document 1 discloses a “biological sample optical scanning device that identifies a biological sample labeled with a fluorescent substance by scanning light on a sample chip on which a large number of biological samples are arranged”.
- the biological sample optical scanning device when a sample chip on which a biological sample is arranged is set on a rotary table, light is swirled with respect to a sample chip that rotates while moving linearly, and a fluorescent substance is detected. The attached biological sample can be detected.
- the apparatus described in Patent Document 1 can irradiate light sequentially to a plurality of areas, and continuously detect a detection target in a sample accommodated in the plurality of areas.
- a series of measurement data such as a series of light intensity data obtained by such continuous light detection
- the main object of the present technology is to provide a data processing apparatus and the like that can accurately specify a range corresponding to a sample storage area in a series of light intensity data.
- the present technology emits the first light intensity distribution data obtained based on the light emitted from the first light source toward the detection region and the second light source toward the detection region.
- a data determination unit that specifies an analysis target range corresponding to a storage area in which the detection target is stored, and an operation mode of the data determination unit are selected
- a mode selection unit wherein the data selection unit is configured to specify the analysis target range from each of the first light intensity distribution data and the second light intensity distribution data. 1 mode and a second mode in which the data determination unit identifies the analysis target range from the second light intensity distribution data based on information on the analysis target range of the first light intensity distribution data.
- the first light intensity distribution data and the second light intensity distribution data are obtained from the light emitted from the first light source and the second light source whose relative positions with respect to the accommodation area change. May be.
- the data processing apparatus includes an input unit to which identification information relating to the specification of the analysis target range is input. When the identification information is input to the input unit, the mode selection unit is configured to input the second mode. May be selected.
- the input unit may be an RF tag or barcode reader.
- the mode selection unit determines whether each value obtained from the first light intensity distribution data and the second light intensity distribution data exceeds a predetermined value, and each of the values is When it is determined that the predetermined value is exceeded, the first mode may be selected.
- the mode selection unit determines that the value obtained from the first light intensity distribution data exceeds the predetermined value, and the value obtained from the second light intensity distribution data does not exceed the predetermined value. In this case, the second mode may be selected.
- the predetermined value may be a value for light intensity, or a value for time or distance.
- the first light intensity distribution data and the second light intensity distribution data may be data acquired in a state where the detection target is not accommodated in the accommodation area.
- the mode selection unit determines whether each value obtained from the first light intensity distribution data and the second light intensity distribution data is less than a predetermined value, and each of the values is When it is determined that the predetermined value is not reached, the first mode may be selected.
- the mode selection unit determines that the value obtained from the first light intensity distribution data is less than the predetermined value, and the value obtained from the second light intensity distribution data is equal to or greater than the predetermined value.
- the second mode may be selected.
- the data determination unit is based on correction information regarding the position of the analysis target range in the second light intensity distribution data with respect to the position of the analysis target range in the first light intensity distribution data.
- the analysis target range of the second light intensity distribution data can be specified.
- the correction information may be a fixed value determined in advance between the first light source and the second light source, and the fixed value is a distance between the first light source and the second light source.
- the fixed value may be based on a difference in time at which each of the first light source and the second light source passes through a predetermined point. .
- the present technology also provides a first light source and a second light source that emit light toward the detection region, and a first light source that is obtained based on the light emitted from the first light source toward the detection region.
- the analysis object For each of the second light intensity distribution data obtained based on the light intensity distribution data and the light emitted from the second light source toward the detection area, the analysis object corresponding to the storage area in which the detection target is stored
- a data processing unit including a data determination unit that specifies a range and a mode selection unit that selects an operation mode of the data determination unit, wherein the data selection unit includes the first data determination unit, The first mode for specifying the analysis target range from each of the light intensity distribution data and the second light intensity distribution data, and the data determination unit relates to the analysis target range of the first light intensity distribution data.
- the first light source and the second light source may be provided in one unit and configured to be movable together.
- Each of the first light source and the second light source may be arranged in a plurality of units, and the units may be configured to be movable independently of each other.
- the data determination unit further emits the first light intensity distribution data obtained based on the light emitted from the first light source toward the detection region and the second light source toward the detection region.
- the mode selection unit is configured so that the data determination unit The first mode for specifying the analysis target range from each of the first light intensity distribution data and the second light intensity distribution data, and the data determination unit, wherein the data determination unit includes the first light intensity distribution data.
- a data processing method for selecting either one of the second modes for specifying the analysis target range from the second light intensity distribution data based on the information on the analysis target range.
- the data determination unit emits the first light intensity distribution data obtained based on the light emitted from the first light source toward the detection region and the second light source toward the detection region.
- Each of the second light intensity distribution data obtained based on the emitted light includes a function of specifying an analysis target range corresponding to a storage area in which the detection target is stored
- the mode selection unit includes the data determination unit The first mode for specifying the analysis target range from each of the first light intensity distribution data and the second light intensity distribution data, and the data determination unit, wherein the data determination unit includes the first light intensity distribution data.
- a data processing program for causing a computer to realize a function of selecting any one of the second modes for specifying the analysis target range from the second light intensity distribution data based on the information on the analysis target range. Also it provides.
- This technology provides a data processing apparatus and the like that can accurately specify a range corresponding to a sample storage area in a series of light intensity data.
- FIG. 1 is a block diagram of an optical detection system according to a first embodiment of the present technology.
- a and B are diagrams illustrating an example of an accommodation area and a detection area in the optical detection system. It is a schematic diagram which shows the structural example of the light source in the optical detection system which concerns on 1st Embodiment.
- FIGS. 8A to 8C are schematic views illustrating a configuration example of a light source in the optical detection system according to the first embodiment. It is a figure which shows the outline
- transformation embodiment of 1st Embodiment. It is a figure showing an example of light intensity distribution data in a data processing method concerning this art. 10 is a flowchart for describing selection of an operation mode in the data processing method according to the present technology.
- a and B are diagrams showing a predetermined value ⁇ 1 and a predetermined value ⁇ 2 in the data processing method according to the present technology.
- a and B are diagrams illustrating an example of light intensity distribution data in the data processing method according to the present technology.
- 6 is a flowchart for explaining a first mode in the data processing method according to the present technology.
- a and B are diagrams for explaining identification of the analysis target range in the first mode.
- 12 is a flowchart for explaining a second mode in the data processing method according to the present technology. It is a figure for demonstrating specification of the analysis object range in the 2nd mode. It is a mimetic diagram showing an example of composition of a light source in an optical detection system concerning a 2nd embodiment of this art. It is a figure which shows the correction information in the optical detection system which concerns on 2nd Embodiment.
- FIG. 1 is a block diagram of an optical detection system A1 according to the first embodiment of the present technology.
- the optical detection system A1 is a system for optically detecting a detection target.
- the detection target is any object that can be optically detected by irradiating light emitted from light sources (first light source 211 and second light source 212) described later. Any of these may be used.
- the detection target include nucleic acids such as DNA and RNA, peptides and proteins, cells, microparticles such as bio-related microparticles and industrial particles, and the like.
- the amplified nucleic acid chain in the nucleic acid amplification reaction can also be detected.
- this label may be the detection target.
- the optical detection system A1 is preferably used for a sample containing two or more types of detection targets because light having different wavelengths is emitted from light sources (first light source and second light source) described later. It is done.
- a sample is, for example, a nucleic acid amplification reaction solution, and the detection target is an amplified nucleic acid chain.
- one detection target included in the sample is a nucleic acid that is unknown whether it is included in the sample, and the other detection target is a nucleic acid that has been confirmed in advance to be included in the sample.
- Each amplified nucleic acid chain is detected using the optical detection system A1 using a fluorescently labeled probe that specifically binds to each of the amplified nucleic acid chains using these nucleic acids as templates.
- the success or failure of the nucleic acid amplification reaction can be determined by detecting the amplification of the nucleic acid that has been confirmed to be contained in the sample in advance. Then, after confirming that the nucleic acid amplification reaction has been performed, the presence or absence of the nucleic acid that is unknown in the sample can be determined.
- the optical detection system A1 includes at least a first light source 211, a second light source 212, a data determination unit 11, and a mode selection unit. 12 is provided. Each configuration of the optical detection system A1 will be described in order.
- the two light sources are the first light source 211 and the second light source 212, but the optical detection system A1 may be provided with three or more light sources. .
- the first light source 211 and the second light source 212 receive light for detecting a detection target (see FIG. 1, arrow light L11, arrow light L12), and a storage area in which the detection target is stored. It is the structure for irradiating toward the detection area containing.
- the first light source 211 and the second light source 212 are light sources that can emit light having different wavelengths.
- the first light source 211 and the second light source 212 can be appropriately selected from those used as known light sources such as a laser light source, an LED light source, a mercury lamp, and a tungsten lamp.
- the accommodation region is a space in which a detection target or a sample including the detection target is stored.
- This accommodation region is, for example, a well provided in the microchip or the inner space of the microtube.
- the detection region is a region including the accommodation region, and corresponds to a region irradiated with the light L11 and L12 emitted from the first light source 211 and the second light source 212. Further, the detection area corresponds to the entire area from which light L21 and L22 detected by the detection unit 3 described later is emitted.
- FIG. 2 is a schematic diagram of the microchip M.
- FIG. 2A is a top view of the microchip M
- FIG. 2B is a cross-sectional view taken along the line PP shown in FIG. 2A.
- each well is a storage region W1, W2, W3 that stores the detection target.
- the area including the accommodation areas W1, W2, and W3 is the detection area D.
- the number of accommodation areas is not particularly limited as long as the detection area D is provided including the accommodation areas W1, W2, and W3.
- the area of the detection region D is preferably larger than the total area of the accommodation regions W1, W2, and W3.
- FIG. 3 is a schematic diagram showing a configuration example of the first light source 211 and the second light source 212 in the optical detection system A1.
- the first light source 211 and the second light source 212 are provided in one unit 21.
- the unit 21 is configured to be movable in the direction indicated by the arrow X1.
- the first light source 211 and the second light source 212 are provided in one unit 21. Therefore, the first light source 211 and the second light source 212 scan the light L11 and L12 by irradiating the light L11 and L12 toward the detection region D while changing the relative position with respect to the detection region D.
- the plurality of storage areas W1, W2, and W3 can be continuously irradiated with light.
- the relative positions of the first light source 211 and the second light source 212 and the detection region D may be changed by moving the microchip M in the direction indicated by the arrow X2 instead of the unit 21.
- FIG. 4 further shows an example of the configuration of the first light source 211 and the second light source 212.
- first light source 211 and the second light source 212 shown in FIGS. 4A to 4C similarly to the configuration of the light source (the first light source 211 and the second light source 212) shown in FIG. are provided with a first light source 211 and a second light source 212.
- the unit 21 rotates around the center of the microchip M formed in a substantially circular shape when viewed from the top, so that the first light source 211 and the second light source 212 are relative to the detection region D surrounded by the broken line.
- the position changes (see arrow X1).
- the microchip M formed in a substantially circular shape when viewed from above is rotated around the axis of the microchip M, whereby the first light source 211 and the second light source 211 for the detection region D surrounded by the broken line
- the relative position of the light source 212 changes (see arrow X2).
- FIG. 4C when the unit 21 rotates about the center of the unit 21, the relative positions of the first light source 211 and the second light source 212 change with respect to the detection region D (see arrow X3).
- the optical detection system A1 including the first light source 211 and the second light source 212 described above may include a configuration provided in a known optical detection device such as an objective lens or a beam splitter.
- the detection unit 3 detects light emitted from the detection region D by irradiation of light from the first light source 211 and the second light source 212 to the detection region D (FIG. 1, arrow lights L21 and 22). It is a configuration. If the detection part 3 can detect the lights L21 and L22 emitted from the detection area D, the structure will not be specifically limited. For example, an area imaging element such as a CCD or CMOS element, a PMT (photomultiplier tube), a photodiode, or the like can be used as the detection unit 3.
- an area imaging element such as a CCD or CMOS element, a PMT (photomultiplier tube), a photodiode, or the like can be used as the detection unit 3.
- the detection part 3 you may be comprised so that both the light L21 and the light L22 may be detected with one detection part 3, and the some detection part 3 detects the light L21 and the light L22, respectively. It may be configured. Further, the detection unit 3 may be configured such that the relative position with respect to the detection region D changes in the optical detection system A1 similarly to the first light source 211 and the second light source 212.
- the data processing device 1 includes a data determination unit 11, a mode selection unit 12, an input unit 13, a CPU 14, a memory 15, a hard disk 16, and the like.
- the data determination unit 11 specifies the analysis target range corresponding to the storage area in which the detection target of the light intensity distribution data is stored.
- the light intensity distribution data is data obtained based on the light emitted toward the detection region D from each of the first light source 211 and the second light source 212 described above.
- the light intensity distribution data and the specification of the analysis target range in the light intensity distribution data will be described later.
- the input unit 13 reads identification information related to specifying the analysis target range, which will be described later.
- this identification information is recorded on, for example, a barcode B (see FIG. 2 again) or an RF tag attached on the microchip M
- the input unit 12 is used as a barcode or RF tag reader.
- the identification information may be directly input to the input unit 13 by the user, and the input unit 13 may be provided with a keyboard or the like so that the user can input the identification information.
- the CPU 14 comprehensively controls each component provided in the data processing apparatus 1. For example, the CPU 14 selects the operation mode of the data determination unit 11 by the mode selection unit 12 described later, and the light intensity by the data determination unit 11. A program that comprehensively controls the identification of the analysis target range in the distribution data is executed.
- the memory 15 is used as a work area of the CPU 14 and temporarily stores light intensity distribution data based on the light detected by the detection unit 3.
- the hard disk 16 stores, for example, measurement data of light detected by the detection unit 3 described above, light intensity distribution data based on the detected light, correction information described later, and the like.
- a computer program for realizing these functions can be created and installed in a personal computer or the like.
- Such a computer program may be stored in a storage medium such as a magnetic disk, an optical disk, a magneto-optical disk, or a flash memory, and may be distributed via a network.
- FIG. 5 shows a configuration of an optical detection system A11 according to a modified embodiment of the first embodiment.
- the data processing device 1 and the detector 3 may be directly connected as shown in FIG. 1, but are connected via the network 4 as shown in FIG. Also good.
- information stored in the hard disk 16 described above may be stored in the server 5.
- the data determination unit 11 and the mode selection unit 12 obtain information from the server 5 via the network 4.
- FIG. 6 is an example of light intensity distribution data stored in the hard disk 16 or the like described above.
- the upper side of FIG. 6 shows the first light intensity distribution data obtained based on the light emitted from the first light source 211 toward the detection region D, and the lower side of FIG.
- region D are shown.
- the light intensity distribution data is a series of light detected by the detection unit 3 along with the scanning of the light emitted from the first light source 211 and the second light source 212 described above.
- Intensity data (light intensity data).
- the light intensity distribution data is data indicating the light intensity distribution in the detection region D.
- the detected light intensity is plotted on the vertical axis, and the detected time is plotted on the horizontal axis.
- the light intensity distribution may be plotted with respect to the detection time as shown in FIG. 6, or may be plotted with respect to the distance the unit 21 or the microchip M has moved. You may plot against the length from one to the other.
- each light intensity distribution data includes a portion corresponding to the light emitted from the accommodation areas W1, W2, and W3 in which the detection target is accommodated. That is, the first light intensity distribution data includes an analysis target range R11 corresponding to the storage area W1, an analysis target range R12 corresponding to the storage area W2, and an analysis target range R13 corresponding to the storage area W3.
- the second light intensity distribution data includes an analysis target range R21 corresponding to the storage area W1, an analysis target range R22 corresponding to the storage area W2, and an analysis target range R23 corresponding to the storage area W3.
- the analysis target ranges R11, R12, R13, R21, R22, and R23 correspond to ranges in which light intensity data derived from the detection target can be included. That is, the analysis target ranges R11, R12, R13, R21, R22, and R23 are portions to be analyzed in the light intensity distribution data for detection of the detection target, quantification of the detection target, and the like.
- the first light intensity distribution data and the second light intensity distribution data described above may be acquired in a state where the detection target is not accommodated in the accommodating areas W1, W2, and W3.
- the degree of attenuation with respect to the intensity of the light L11, L12 at the time of emission after the light L11, L12 emitted from each of the first light source 211 and the second light source 212 passes through the detection region D.
- the light intensity distribution data does not include light derived from the detection target, the light intensity and the like are more uniform between the accommodation areas W1, W2, and W3. For this reason, in the data processing method to be described later, the analysis target ranges R11, R12, R13, R21, R22, and R23 corresponding to the plurality of accommodation areas W1, W2, and W3 included in the light intensity distribution data are specified under the same conditions. Easy to do. Therefore, the light intensity distribution data acquired in a state where the detection target is not stored in the storage areas W1, W2, and W3 is the analysis target ranges R11, R12, R13, R21, R22, and R23 by the data processing method according to the present technology. It is suitably used for specifying.
- the information on the analysis target ranges R11, R12, R13, R21, R22, and R23 of the first light intensity distribution data and the second light intensity distribution data is obtained as the light intensity distribution data obtained after the detection target is accommodated.
- the lights L11 and L12 may be excitation light for the detection target, and light having a wavelength corresponding to the absorption wavelength of the detection target. There may be, and it is not specifically limited.
- the data processing method according to the present technology includes a step in which the mode selection unit 12 selects one of a first mode and a second mode, which will be described later, as the operation mode of the data determination unit 11. Further, the data processing method according to the present technology includes a step in which the data determination unit 11 specifies the analysis target ranges R11, R12, R13, R21, R22, and R23 in the light intensity distribution data according to the selected operation mode. Including.
- the flowchart shown in FIG. 7 shows each process in the selection of the operation mode by the mode selection unit 12.
- the mode selection unit 12 may confirm whether or not the identification information is input in step S0.
- the mode selection unit 12 selects the second mode (step S1-5).
- the mode selection unit 12 performs step S1-1.
- step S1-1 the mode selection unit 12 determines whether each value obtained from the first light intensity distribution data and the second light intensity distribution data exceeds a predetermined value.
- the value obtained from the light intensity distribution data is, for example, the intensity of detection light or the detection time.
- the predetermined value is a value set in advance. This predetermined value may be a value for light intensity, for example.
- the predetermined value can be a value for time or distance.
- FIG. 8 shows an example of predetermined values in the data processing method according to the present technology.
- the predetermined value ⁇ 1 is a predetermined value with respect to the detected light intensity (light intensity).
- the predetermined value ⁇ 2 is a predetermined value for the time (time t1 to time t2) when the light intensity exceeding the predetermined value ⁇ 1 is continuously detected.
- one predetermined value such as a predetermined value ⁇ 1 may be used as the predetermined value.
- a plurality of predetermined values such as the predetermined value ⁇ 1 and the predetermined value ⁇ 2 may be used in combination.
- FIG. 8A shows an example in which the value obtained from the light intensity distribution data exceeds the predetermined value ⁇ 1 and the predetermined value ⁇ 2. In this case, the mode selection unit 12 determines that the value obtained from the light intensity distribution data exceeds a predetermined value.
- FIG. 8B shows an example in which the value obtained from the light intensity distribution data does not exceed the predetermined value ⁇ 1 and the predetermined value ⁇ 2. In this case, the mode selection unit 12 determines that the value obtained from the light intensity distribution data does not exceed the predetermined value.
- step S1-2 the mode selection unit 12 sets a value obtained from each of the first light intensity distribution data and the second light intensity distribution data to a predetermined value based on the determination result of step S1-1. If it exceeds (Yes), the first mode is selected (step S1-3). The first mode will be described later.
- step S1-4 the mode selection unit 12 determines that the value obtained from the first light intensity distribution data exceeds the predetermined value based on the determination result of step S1-1, and the value obtained from the second light intensity distribution data. If the predetermined value does not exceed the predetermined value (Yes), the second mode is selected (step S1-5).
- the first light intensity distribution data is not particularly limited as long as it is data obtained from light emitted from either of the two light sources provided in the optical detection system A1. Therefore, the mode selection unit 12 selects the second mode when the value obtained from any one of the light intensity distribution data exceeds a predetermined value.
- the mode selection unit 12 selects the operation mode without End mode selection. This means an error end.
- the data determination unit 11 may include the function of selecting the operation mode of the mode selection unit 12 described above.
- the first light intensity distribution data and the second light intensity distribution data are determined based on the wavelengths of the light L11, L12, whether the detection target is stored in the storage areas W1, W2, W3, and the like.
- R12, R13, R21, R22, and R23 may have a high light intensity as shown in FIG. 8 or a low value as shown in FIG. 9 with respect to other regions of the light intensity distribution data.
- FIG. 9 is an example of the first light intensity distribution data (FIG. 9A) and the second light intensity distribution data (FIG. 9B) obtained based on the absorbance of the lights L11 and L12.
- the analysis target ranges R11, R12, R13, R21, R22, and R23 have lower light intensity than other areas of the data.
- the mode selection unit 12 uses the predetermined value ⁇ 1 for the light intensity
- the first light intensity distribution data and the second light intensity distribution data It is determined whether or not each value obtained from the light intensity distribution data is less than a predetermined value ⁇ 1.
- the predetermined value ⁇ 1 is as described above.
- the mode selection unit 12 determines that none of these values is less than the predetermined value ⁇ 1, the mode selection unit 12 selects the first mode. Further, when the mode selection unit 12 determines that the value obtained from the first light intensity distribution data is less than the predetermined value ⁇ 1, and the value obtained from the second light intensity distribution data is equal to or greater than the predetermined value ⁇ 1, Select the second mode.
- the mode selection unit 12 selects the second mode.
- the data determination unit 11 includes accommodation areas W1, W2, and W3 in which detection targets of light intensity distribution data are accommodated.
- the analysis object ranges R11, R12, R13, R21, R22, and R23 corresponding to are specified.
- the flowchart shown in FIG. 10 shows each step in the first mode in the data processing method according to the present technology.
- the data determination unit 11 specifies the analysis target ranges R11, R12, and R13 from the first light intensity distribution data (step S2-1). Furthermore, the data determination unit 11 specifies analysis target ranges R21, R22, and R23 from the second light intensity distribution data (step S-2). That is, in the first mode, the data determination unit 11 does not use the information on the analysis target ranges R11, R12, and R13 of the first light intensity distribution data, and the analysis target range for the second light intensity distribution data. R21, R22, R23 are specified.
- the data determination unit 11 can also use the predetermined values ⁇ 1 and ⁇ 2 described above. Further, the predetermined values ⁇ 1 and ⁇ 2 may be the same as the values used in the mode selection by the mode selection unit 12 (see FIG. 7). Further, in addition to the predetermined values ⁇ 1 and ⁇ 2, for example, the light intensity distribution data may be plotted as shown in FIG. 6 and the analysis target range may be specified from the waveform.
- FIG. 11A shows an example of step S2-1.
- FIG. 11B shows an example of step S2-2.
- the data determination unit 11 selects a range (time t1 to time t2, time t5 to time t6, time t9 to time t10) exceeding the predetermined value ⁇ 1 of the first light intensity distribution data as the analysis target range R11, These are specified as R12 and R13 (FIG. 11A).
- the data determination unit 11 determines a range exceeding the predetermined value ⁇ 1 (time t3 to time t4, time t7 to time t8, time t11 to time t12) based on the predetermined value ⁇ 1.
- the analysis target ranges are R21, R22, and R23 (FIG. 11B).
- region made into analysis object range R11, R12, R13, R21, R22, R23 has low light intensity with respect to the other area
- a range less than ⁇ 1 can be set as the analysis target range.
- the predetermined value ⁇ 1 for the first light intensity distribution data and the predetermined value ⁇ 1 for the second light intensity distribution data are the characteristics of the first light source 211 and the characteristics of the second light source 212. It is preferable to use different values in consideration of the difference between the two. It should be noted that the same value can be used for both the first light intensity distribution data and the second light intensity distribution data for the predetermined value ⁇ 1.
- the predetermined value ⁇ 2 with respect to time or distance since the predetermined value ⁇ 2 is a value corresponding to the size of the accommodation areas W1, W2, and W3, the predetermined value ⁇ 2 with respect to the first light intensity distribution data and the second It is preferable to use the same value as the predetermined value ⁇ 2 for the light intensity distribution data.
- a different value may be used as the predetermined value ⁇ 2 for each of the first light intensity distribution data and the second light intensity distribution data.
- the predetermined value ⁇ 1 for specifying the analysis target ranges R11, R12, R13, R21, R22, R23 may be one. Further, similarly to the mode selection unit 12, the data determination unit 11 may use a plurality of predetermined values ⁇ 1, ⁇ 2 in order to specify the analysis target ranges R11, R12, R13, R21, R22, R23 (FIG. 8B). reference).
- FIG. 12 shows each step of the second mode in the data processing method according to the present technology.
- the data determination unit 11 specifies the analysis target ranges R11, R12, and R13 from the first light intensity distribution data in the same manner as in the step S2-1 in the first mode (step S3- 1).
- the data determination unit 11 is based on the analysis target range of the first light intensity distribution data.
- Analysis target ranges R21, R22, and R23 are specified for the second light intensity distribution data (step S3-2).
- the data determination unit 11 performs the analysis target ranges R21, R22, R23 in the second light intensity distribution data with respect to the positions of the analysis target ranges R11, R12, R13 in the first light intensity distribution data.
- the analysis target ranges R21, R22, and R23 of the second light intensity distribution data can also be specified based on the correction information regarding the position of.
- the correction information is preferably a fixed value determined in advance between the first light source 211 and the second light source 212.
- the fixed value is, for example, the distance between the first light source 211 and the second light source 212, the speed at which the relative position changes, and the like.
- the unit 21 shown in FIG. 13 is provided with a first light source 211 and a second light source 212, and the distance d between them is a fixed value.
- the unit 21 moves in the direction indicated by the arrow X1, and each of the light emitted from the first light source 211 and the second light source 212 is sequentially irradiated onto the accommodation areas W1, W2, and W3 (in FIG. 13, the accommodation areas W1, W2). , W3 is not shown). Therefore, the start position of the analysis target range R11 in the first light intensity distribution data corresponding to the storage area W1 and the start position of the analysis target range R21 in the second light intensity distribution data corresponding to the storage area W1. There is a time difference ( ⁇ t) between them.
- the time difference ( ⁇ t) is a value that can be calculated from the distance d between the light sources and the moving speed of the unit 21. Therefore, the fixed value based on the distance between the first light source 211 and the second light source 212 and the speed at which the relative position with respect to the detection region changes is specified as the analysis target ranges R21, R22, and R23 in the second light intensity distribution data. Can be used as correction information. select.
- the optical detection system A1 When the optical detection system A1 is provided with three or more light sources and the value obtained from the plurality of light intensity distribution data exceeds a predetermined value, the plurality of original values obtained from the values determined to exceed the predetermined value are obtained. It is preferable to select reference light intensity distribution data from the light intensity distribution data. Regarding the selection of the reference, for example, a plurality of predetermined values for light intensity, time, etc. may be used, and the light intensity distribution data determined to exceed the most predetermined value may be used as the reference. In addition, the data determination unit 11 may have this reference selection function. Further, in the case of the light source configuration described above, even if the second mode is selected, the data determination unit 11 determines each of the plurality of light intensity distribution data determined to exceed a predetermined value. The analysis target range may be specified without using the above-described method described later.
- the identification information related to specifying the analysis target range may include, for example, information about the first light source 211. According to the information about the first light source 211, the data determination unit 11 determines, for example, which wavelength light source emits the first light source 211.
- the above identification information may include, for example, the above-described predetermined value and correction information.
- the data determination unit 11 converts the light intensity distribution data (first light intensity distribution data) obtained from the light emitted from the light source (first light source 211) determined by the identification information in step S3-1.
- the analysis target range can be specified based on a predetermined value recorded in the identification information.
- the data determination unit 11 receives from another light source (second light source) based on the information regarding the analysis target range obtained in step S3-1 and the correction information recorded in the identification information.
- the analysis target range can be specified for the light intensity distribution data (second light intensity distribution) obtained from the emitted light.
- detection is performed for each of the first light intensity distribution data and the second light intensity distribution data obtained by the light emitted from each of the first light source 211 and the second light source 212.
- the analysis target range corresponding to the area in which the target is accommodated is specified.
- the mode selection unit 12 selects one of the following two modes.
- the data determination unit 11 specifies the analysis target ranges R11, R12, R13, R21, R22, and R23 based on predetermined values for the first light intensity distribution data and the second light intensity distribution data. To do.
- the analysis target ranges R21, R22, R23 with respect to the second light intensity distribution data based on the information about the analysis target ranges R11, R12, R13 of the first light intensity distribution data. Is identified.
- the optical characteristics of the materials constituting the housing regions W1, W2, and W3 such as the microchip M may be different.
- conditions at the time of detection of the detection target by the optical detection system A1 such as temperature and humidity, deviation of the optical axis accompanying use, etc. It may be difficult to specify the analysis target range with high accuracy. In such a case, it is preferable to specify the analysis target ranges R11, 12, 13, 21, 22, and 23 independently for each of the light intensity distribution data obtained by irradiating light from a plurality of light sources. Therefore, the first mode is preferable.
- the optical detection system A1 when a light source that cannot obtain sufficient light intensity for specifying the analysis target ranges R21, R22, and R23 is provided in the optical detection system A1, the light intensity distribution obtained by the light emitted from the light source.
- the second mode is preferable.
- the mode selection unit 12 can select an optimal operation mode. For this reason, the analysis object ranges R11, R12, R13, R21, R22, and R23 in the light intensity distribution data can be specified with higher accuracy.
- the analysis target ranges R21, R22, and R23 are specified for the second light intensity distribution data. become unable.
- the data determination unit 11 selects the analysis target range R21 from the light intensity distribution data by selecting the second mode. , R22, R23 can be specified.
- FIG. 14 is a schematic diagram of the first light source 211 and the second light source 212 in the optical detection system A2 according to the second embodiment of the present technology.
- the configuration other than the units 21a and 21b is the same as that in the first embodiment.
- the same components as those in the first embodiment are denoted by the same reference numerals, and description of overlapping portions is omitted.
- the first light source 211 and the second light source 212 are provided in the unit 21a and the unit 21b, respectively.
- the first light source 211 and the second light source 212 may be disposed in the plurality of units 21a and 21b. Further, since these units 21a and 21b can move independently from each other, the relative positions of the first light source 211 and the second light source 212 with respect to the detection region D change independently of each other (FIG. 14). , See arrows X1 and X2. Detection region D is not shown in FIG.
- the correction information may be a fixed value based on the difference in time at which each of the first light source 211 and the second light source 212 passes a predetermined point (reference point). This is because the difference between the start positions of the analysis target ranges R11 and R21 corresponding to the same accommodation area W1 in the light intensity distribution data is the time t1 when the first light source 211 passes the reference point and the reference of the second light source 212. This is because it is based on the difference from the time t2 passing the point.
- the data determination unit 11 may use correction information based on the difference between the movement start positions of the plurality of units 21a and 21b. Further, the optical detection system A2 may be provided with position sensors for the units 21a and 21b, and the data determination unit 11 may use correction information based on position information obtained from the position sensors.
- the first light source 211 and the second light source 212 are configured such that reflected light from the detection region D based on the emitted light (light L11, light L12) from each enters the detection unit 3. It is preferable to be configured not to emit light. In order to prevent the reflected light from entering, for example, the light (lights L11 and L12) is simultaneously transmitted from the two light sources to the detection region D by shifting the light emission timings of the first light source 211 and the second light source 212. You may be comprised so that it may not radiate
- each of the first light source 211 and the second light source 212 is disposed in the plurality of units 21a and 21b. For this reason, the first light source 211 and the second light source 212 are moved independently, and the light L11 from the first light source 211 and the light L12 from the second light source 212 are simultaneously changed to different storage areas W1, It is also possible to irradiate W2 and W3. Therefore, it is possible to shorten the time required for acquiring the light intensity distribution data by the optical detection system A2. Further, in the optical detection system A2, when the type of the light source (the first light source 211, the second light source 212) is changed or added, only the necessary light source can be changed or added. The cost required for this is low. The same applies to the maintenance of the light sources (the first light source 211 and the second light source 212), for example. Other effects of the optical detection system A2 are the same as those of the optical detection system A1 according to the first embodiment.
- this technique can also take the following structures. (1) Obtained based on the first light intensity distribution data obtained based on the light emitted from the first light source toward the detection area and the light emitted from the second light source toward the detection area. For each of the second light intensity distribution data, a data determination unit that specifies an analysis target range corresponding to a storage area in which a detection target is stored, and a mode selection unit that selects an operation mode of the data determination unit.
- the mode selection unit includes a first mode in which the data determination unit specifies the analysis target range from each of the first light intensity distribution data and the second light intensity distribution data; The data determination unit selects any one of the second modes in which the analysis target range is specified from the second light intensity distribution data based on information on the analysis target range of the first light intensity distribution data.
- the data processing device to select.
- the first light intensity distribution data and the second light intensity distribution data are obtained from the light emitted from the first light source and the second light source whose relative positions with respect to the accommodation area change.
- the data processing apparatus according to (1) above.
- An input unit for inputting identification information relating to the specification of the analysis target range is provided, and when the identification information is input to the input unit, the mode selection unit selects the second mode.
- the data processing device according to (1) or (2) above.
- the data processing apparatus according to (3), wherein the input unit is an RF tag or barcode reader.
- the mode selection unit determines whether or not each value obtained from the first light intensity distribution data and the second light intensity distribution data exceeds a predetermined value. 5.
- the data processing apparatus according to any one of (1) to (4), wherein when it is determined that the first exceeds the predetermined value, the first mode is selected.
- the mode selection unit the value obtained from the first light intensity distribution data exceeds the predetermined value, and the value obtained from the second light intensity distribution data does not exceed the predetermined value.
- the data processing apparatus according to (5), wherein the second mode is selected when it is determined.
- the data processing device (7) The data processing device according to (5) or (6), wherein the predetermined value is a value with respect to light intensity.
- the data processing device (8) The data processing device according to (5) or (6), wherein the predetermined value is a value with respect to time or distance.
- the mode selection unit determines whether or not each value obtained from the first light intensity distribution data and the second light intensity distribution data is less than a predetermined value.
- the data processing apparatus according to (9), wherein when it is determined that none of the values is less than the predetermined value, the first mode is selected.
- the mode selection unit In the mode selection unit, the value obtained from the first light intensity distribution data is less than the predetermined value, and the value obtained from the second light intensity distribution data is not less than the predetermined value.
- the data determination unit corrects the position of the analysis target range in the second light intensity distribution data with respect to the position of the analysis target range in the first light intensity distribution data.
- the data processing apparatus according to (2) wherein the analysis target range of the second light intensity distribution data is specified based on information.
- the correction information is a fixed value determined in advance between the first light source and the second light source.
- the data processing device (14) The data processing device according to (13), wherein the fixed value is based on a distance between the first light source and the second light source and a speed at which the relative position changes. (15) The data processing device according to (13), wherein the fixed value is based on a difference in time at which each of the first light source and the second light source passes through a predetermined point. (16) The first light intensity distribution obtained based on the first light source and the second light source that emit light toward the detection region, and the light emitted from the first light source toward the detection region.
- the analysis target range corresponding to the storage region in which the detection target is stored is specified
- a data processing device having a mode selection unit that selects an operation mode of the data determination unit, wherein the data determination unit includes the first light intensity distribution.
- the second Optical detection system for selecting the second mode for specifying the analyzed range of the light intensity distribution data, either.
- the first mode for specifying the analysis target range from each of the first light intensity distribution data and the second light intensity distribution data, and the data determination unit includes the analysis target range of the first light intensity distribution data.
- a function for specifying the analysis target range corresponding to the storage area in which the detection target is stored is included, and the mode selection unit includes the data determination unit, The first mode for specifying the analysis target range from each of the first light intensity distribution data and the second light intensity distribution data, and the data determination unit includes the analysis target range of the first light intensity distribution data.
- A1, A2, A11 Optical detection system
- B Bar code
- D Detection area
- M Microchip
- R11, R12, R13, R21, R22, R23 Analysis target range
- W1, W2, W3 Containment area
- 1 data processing device
- 11 data determination unit
- 12 mode selection unit
- 13 input unit
- 14 CPU
- 15 memory
- 16 hard disk
- 2 light source
- 21, 21a, 21b unit
- 212 second light source
- 3 detection unit
- 4 network
- 5 server
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Abstract
Description
前記第1の光強度分布データ及び前記第2の光強度分布データは、前記収容領域との相対位置が変化する前記第1の光源及び第2の光源から出射された光によって得られるものであってもよい。
前記データ処理装置は、前記解析対象範囲の特定に関する識別情報が入力される入力部を有し、該入力部に前記識別情報が入力された場合には、前記モード選択部は前記第2のモードを選択してもよい。
前記入力部は、RFタグ又はバーコードの読み取り機とすることもできる。
前記モード選択部は、前記第1の光強度分布データ及び前記第2の光強度分布データから得られる各々の値が所定値を超えるか否かを判定し、前記各々の値の何れもが前記所定値を超えていると判定した場合、前記第1のモードを選択してもよい。
前記モード選択部は、前記第1の光強度分布データから得られる前記値が前記所定値を超え、前記第2の光強度分布データから得られる前記値が前記所定値を超えていないと判定した場合、前記第2のモードを選択してもよい。
前記所定値は、光強度に対する値や、時間又は距離に対する値とすることができる。
前記第1の光強度分布データ及び第2の光強度分布データは、前記検出対象が前記収容領域に収容されていない状態で取得されたデータであってもよい。
前記モード選択部は、前記第1の光強度分布データ及び前記第2の光強度分布データから得られる各々の値が所定値に満たないか否かを判定し、前記各々の値の何れもが前記所定値に満たないと判定した場合、前記第1のモードを選択してもよい。
前記モード選択部は、前記第1の光強度分布データから得られる前記値が前記所定値に満たず、前記第2の光強度分布データから得られる前記値が前記所定値以上であると判定した場合、前記第2のモードを選択してもよい。
前記データ判定部は、前記第2のモードにおいて、前記第1の光強度分布データにおける前記解析対象範囲の位置に対する、前記第2の光強度分布データにおける前記解析対象範囲の位置に関する補正情報に基づき、前記第2の光強度分布データの前記解析対象範囲を特定することもできる。
前記補正情報は、前記第1の光源及び前記第2の光源の間で予め定められた固定値であってもよく、前記固定値は、前記第1の光源と第2の光源の間の距離、及び前記相対位置が変化する速度に基づいていてもよく、前記固定値は、前記第1の光源及び前記第2の光源の各々が所定の地点を通過する時刻の差に基づいていてもよい。
前記第1の光源及び前記第2の光源は、一のユニットに備えられ、一体で移動可能に構成されていてもよい。
また、前記第1の光源及び前記第2の光源の各々は、複数のユニットに配設され、該ユニットは、互いに独立して移動可能に構成されていてもよい。
本技術の第1実施形態に係る光学検出システムについて、図1~図4を参照しながら説明する。図1は、本技術の第1実施形態に係る光学検出システムA1のブロック図である。光学検出システムA1は、検出対象を光学的に検出するためのシステムである。光学検出システムA1において、検出対象とは、後述する光源(第1の光源211と第2の光源212)から出射される光を照射することによって、光学的に検出することができるものであれば、何れであってもよい。検出対象としては、例えば、DNA、RNA等の核酸、ペプチドやタンパク質、細胞、生体関連微小粒子や工業用粒子等の微小粒子、などが挙げられる。また、核酸については、核酸増幅反応における増幅核酸鎖を検出対象とすることもできる。さらに、これらの検出対象に蛍光などの標識が付されている場合には、この標識を検出対象としてもよい。
図1に示すように、光学検出システムA1は、少なくとも、第1の光源211と第2の光源212と、データ判定部11とモード選択部12とを有するデータ処理装置1と、を備える。光学検出システムA1の各構成について、順に説明する。
第1の光源211及び第2の光源212は、光学検出システムA1において、検出対象を検出するための光(図1、矢印光L11,矢印光L12参照)を、検出対象が収容される収容領域を含む検出領域に向けて照射するための構成である。第1の光源211及び第2の光源212は、互いに異なる波長の光を出射可能な光源である。第1の光源211及び第2の光源212には、例えば、レーザ光源、LED光源、水銀ランプ、タングステンランプ等の公知の光源として用いられるものの中から適宜選択することができる。
検出部3は、第1の光源211と第2の光源212からの検出領域Dへの光の照射によって検出領域Dから出射される光(図1、矢印光L21,22)を検出するための構成である。検出部3は、検出領域Dから出射される光L21,L22を検出することが可能であれば、その構成は、特に限定されない。例えば、検出部3として、CCDやCMOS素子等のエリア撮像素子、PMT(光電子倍増管)、フォトダイオード等を用いることができる。検出部3については、一つの検出部3で光L21と光L22の両方を検出するように構成されていてもよく、複数の検出部3が、各々、光L21と光L22を検出するように構成されていてもよい。また、検出部3は、光学検出システムA1において、第1の光源211や第2の光源212と同様に、検出領域Dに対して相対位置が変化するように構成されていてもよい。
データ処理装置1は、図1に示すように、データ判定部11、モード選択部12、入力部13、CPU14、メモリ15、ハードディスク16などで構成されている。
データ判定部11は、光強度分布データの検出対象が収容される収容領域に対応する解析対象範囲を特定する。光強度分布データは、上述した第1の光源211及び第2の光源212の各々から検出領域Dへ向けて出射された光に基づいて得られるデータである。光強度分布データと、光強度分布データにおける解析対象範囲の特定については、後述する。
入力部13は、後述する解析対象範囲の特定に関する識別情報を読み取る。この識別情報が、例えばマイクロチップM上に付されたバーコードB(図2再度参照)やRFタグに記録されている場合には、入力部12を、バーコードやRFタグの読み取り機とすることもできる。また、識別情報は、ユーザが直接入力部13へ入力してもよく、入力部13にはユーザが識別情報を入力できるようにキーボードなどが備えられていてもよい。
CPU14は、データ処理装置1に設けられた各構成を統括的に制御するものであり、例えば、後述するモード選択部12によるデータ判定部11の動作モードの選択や、データ判定部11による光強度分布データにおける解析対象範囲の特定を統括的に制御するプログラムを実行する。
メモリ15は、CPU14の作業領域として用いられ、検出部3で検出された光に基づく光強度分布データなどを一時的に記憶する。
ハードディスク16は、例えば、上述した検出部3において検出された光の測定データや検出された光に基づく光強度分布データ、後述する補正情報などが記憶される。
本技術の第1実施形態に係る光学検出システムA1を用いて、データを処理する方法について説明する。先ず、本技術に係るデータ処理方法における光強度分布データについて、図6を参照しながら説明する。なお、以下のデータ処理方法の説明においては、便宜的に、図2に示すマイクロチップMを用いた場合を例に説明する。
図6は、上述したハードディスク16などに記憶されている光強度分布データの一例である。図6の上側は、第1の光源211から検出領域Dに向けて出射された光に基づいて得られる第1の光強度分布データを示し、図6の下側は、第2の光源212から検出領域Dに向けて出射された光に基づいて得られる第2の光強度分布データを示す。
次に、フローチャート(図7、図10、図12)を参照しながら本技術に係るデータ処理方法について説明する。本技術に係るデータ処理方法は、モード選択部12が、データ判定部11の動作モードについて、後述する第1のモードと第2のモードの何れか一方を選択する工程を含む。また、本技術に係るデータ処理方法は、データ判定部11が、選択された動作モードに応じて、光強度分布データにおいて解析対象範囲R11,R12,R13,R21,R22,R23を特定する工程を含む。
図7に示すフローチャートは、モード選択部12による動作モードの選択における各工程を示す。上述した光検出システムA1に入力部13が備えられている場合には、モード選択部12は、工程S0において、識別情報の入力の有無を確認してもよい。入力部13へ解析対象範囲の特定に関する識別情報が入力された場合には、モード選択部12は、第2モードを選択する(工程S1-5)。一方、識別情報が入力されなかった場合には、モード選択部12は、工程S1-1を行う。
上述したモード選択部12によって選択された動作モード(第1のモード又は第2のモード)に応じて、データ判定部11は光強度分布データの検出対象が収容される収容領域W1,W2,W3に対応する解析対象範囲R11,R12,R13,R21,R22,R23を特定する。
図10に示すフローチャートは、本技術に係るデータ処理方法における第1のモードの各工程を示す。第1のモードにおいてデータ判定部11は、第1の光強度分布データから解析対象範囲R11,R12,R13を特定する(工程S2-1)。さらに、データ判定部11は、第2の光強度分布データから、解析対象範囲R21,R22,R23を特定する(工程S-2)。即ち、第1のモードでは、データ判定部11は、第1の光強度分布データの解析対象範囲R11,R12,R13に関する情報を用いることなく、第2の光強度分布データに対して解析対象範囲R21,R22,R23を特定する。
図12に示すフローチャートは、本技術に係るデータ処理方法における第2のモードの各工程を示す。データ判定部11は、第2のモードにおいても、第1のモードの工程S2-1と同様に、第1の光強度分布データから、解析対象範囲R11,R12,R13を特定する(工程S3-1)。
選択する。
上述したように、入力部13へ解析対象範囲の特定に関する識別情報が入力された場合にも、モード選択部12は第2のモードを選択し、データ判定部11は図12に示す各工程によって解析対象範囲を特定する。
図14は、本技術の第2実施形態に係る光学検出システムA2における第1の光源211と第2の光源212の模式図である。光学検出システムA2において、ユニット21a,21b以外の構成は、第1実施形態と同一である。第1実施形態と同一の構成については、同一の符号を付し、重複する部分の説明は省略する。
(1)第1の光源から検出領域に向けて出射された光に基づいて得られる第1の光強度分布データと第2の光源から前記検出領域に向けて出射された光に基づいて得られる第2の光強度分布データの各々について、検出対象が収容される収容領域に対応する解析対象範囲を特定するデータ判定部と、該データ判定部の動作モードを選択するモード選択部と、を有し、前記モード選択部は、前記データ判定部が、前記第1の光強度分布データ及び前記第2の光強度分布データの各々から、前記解析対象範囲を特定する第1のモードと、
前記データ判定部が、前記第1の光強度分布データの前記解析対象範囲に関する情報に基づき、前記第2の光強度分布データから前記解析対象範囲を特定する第2のモードの、何れか一方を選択するデータ処理装置。
(2)前記第1の光強度分布データ及び前記第2の光強度分布データは、前記収容領域との相対位置が変化する前記第1の光源及び第2の光源から出射された光によって得られる上記(1)に記載のデータ処理装置。
(3)前記解析対象範囲の特定に関する識別情報が入力される入力部を有し、該入力部に前記識別情報が入力された場合には、前記モード選択部は前記第2のモードを選択する上記(1)又は(2)に記載のデータ処理装置。
(4)前記入力部は、RFタグ又はバーコードの読み取り機である上記(3)に記載のデータ処理装置。
(5)前記モード選択部は、前記第1の光強度分布データ及び前記第2の光強度分布データから得られる各々の値が所定値を超えるか否かを判定し、前記各々の値の何れもが前記所定値を超えていると判定した場合、前記第1のモードを選択する上記(1)~(4)のいずれかに記載のデータ処理装置。
(6)前記モード選択部は、前記第1の光強度分布データから得られる前記値が前記所定値を超え、前記第2の光強度分布データから得られる前記値が前記所定値を超えていないと判定した場合、前記第2のモードを選択する上記(5)に記載のデータ処理装置。
(7)前記所定値は、光強度に対する値である上記(5)又は(6)に記載のデータ処理装置。
(8)前記所定値は、時間又は距離に対する値である上記(5)又は(6)に記載のデータ処理装置。
(9)前記第1の光強度分布データ及び第2の光強度分布データは、前記検出対象が前記収容領域に収容されていない状態で取得されたデータである上記(1)に記載のデータ処理装置。
(10)前記モード選択部は、前記第1の光強度分布データ及び前記第2の光強度分布データから得られる各々の値が所定値に満たないか否かを判定し、前記各々の値の何れもが前記所定値に満たないと判定した場合、前記第1のモードを選択する上記(9)に記載のデータ処理装置。
(11)前記モード選択部は、前記第1の光強度分布データから得られる前記値が前記所定値に満たず、前記第2の光強度分布データから得られる前記値が前記所定値以上であると判定した場合、前記第2のモードを選択する上記(10)に記載のデータ処理装置。
(12)前記データ判定部は、前記第2のモードにおいて、前記第1の光強度分布データにおける前記解析対象範囲の位置に対する、前記第2の光強度分布データにおける前記解析対象範囲の位置に関する補正情報に基づき、前記第2の光強度分布データの前記解析対象範囲を特定する上記(2)に記載のデータ処理装置。
(13)前記補正情報は、前記第1の光源及び前記第2の光源の間で予め定められた固定値である上記(12)に記載のデータ処理装置。
(14)前記固定値は、前記第1の光源と第2の光源の間の距離、及び前記相対位置が変化する速度に基づく上記(13)に記載のデータ処理装置。
(15)前記固定値は、前記第1の光源及び前記第2の光源の各々が所定の地点を通過する時刻の差に基づく上記(13)に記載のデータ処理装置。
(16)検出領域に向けて光を出射する第1の光源及び第2の光源と、前記第1の光源から前記検出領域に向けて出射された光に基づいて得られる第1の光強度分布データと前記第2の光源から前記検出領域に向けて出射された光に基づいて得られる第2の光強度分布データの各々について、検出対象が収容される収容領域に対応する解析対象範囲を特定するデータ判定部と、該データ判定部の動作モードを選択するモード選択部と、を有するデータ処理装置と、を備え、該モード選択部は、前記データ判定部が、前記第1の光強度分布データ及び前記第2の光強度分布データの各々から、前記解析対象範囲を特定する第1のモードと、前記データ判定部が、前記第1の光強度分布データの前記解析対象範囲に関する情報に基づき、前記第2の光強度分布データから前記解析対象範囲を特定する第2のモードの、何れか一方を選択する光学検出システム。
(17)前記第1の光源及び前記第2の光源は、一のユニットに備えられ、一体で移動可能に構成されている上記(16)に記載の光学検出システム。
(18)前記第1の光源及び前記第2の光源の各々は、複数のユニットに配設され、該ユニットは、互いに独立して移動可能に構成されている上記(16)に記載の光学検出システム。
(19)データ判定部が、第1の光源から検出領域に向けて出射された光に基づいて得られる第1の光強度分布データと第2の光源から前記検出領域に向けて出射された光に基づいて得られる第2の光強度分布データの各々について、検出対象が収容される収容領域に対応する解析対象範囲を特定することを含み、モード選択部は、前記データ判定部が、前記第1の光強度分布データ及び第2の光強度分布データの各々から、前記解析対象範囲を特定する第1のモードと、前記データ判定部が、前記第1の光強度分布データの前記解析対象範囲に関する情報に基づき、前記第2の光強度分布データから前記解析対象範囲を特定する第2のモードの、何れか一方を選択するデータ処理方法。
(20)データ判定部が、第1の光源から検出領域に向けて出射された光に基づいて得られる第1の光強度分布データと第2の光源から前記検出領域に向けて出射された光に基づいて得られる第2の光強度分布データの各々について、検出対象が収容される収容領域に対応する解析対象範囲を特定する機能を含み、モード選択部は、前記データ判定部が、前記第1の光強度分布データ及び第2の光強度分布データの各々から、前記解析対象範囲を特定する第1のモードと、前記データ判定部が、前記第1の光強度分布データの前記解析対象範囲に関する情報に基づき、前記第2の光強度分布データから前記解析対象範囲を特定する第2のモードの、何れか一方を選択する機能をコンピュータに実現させるためのデータ処理プログラム。
Claims (20)
- 第1の光源から検出領域に向けて出射された光に基づいて得られる第1の光強度分布データと第2の光源から前記検出領域に向けて出射された光に基づいて得られる第2の光強度分布データの各々について、検出対象が収容される収容領域に対応する解析対象範囲を特定するデータ判定部と、
該データ判定部の動作モードを選択するモード選択部と、を有し、
前記モード選択部は、前記データ判定部が、前記第1の光強度分布データ及び前記第2の光強度分布データの各々から、前記解析対象範囲を特定する第1のモードと、
前記データ判定部が、前記第1の光強度分布データの前記解析対象範囲に関する情報に基づき、前記第2の光強度分布データから前記解析対象範囲を特定する第2のモードの、何れか一方を選択する
データ処理装置。 - 前記第1の光強度分布データ及び前記第2の光強度分布データは、前記収容領域との相対位置が変化する前記第1の光源及び第2の光源から出射された光によって得られる
請求項1に記載のデータ処理装置。 - 前記解析対象範囲の特定に関する識別情報が入力される入力部を有し、該入力部に前記識別情報が入力された場合には、前記モード選択部は前記第2のモードを選択する
請求項1に記載のデータ処理装置。 - 前記入力部は、RFタグ又はバーコードの読み取り機である
請求項3に記載のデータ処理装置。 - 前記モード選択部は、前記第1の光強度分布データ及び前記第2の光強度分布データから得られる各々の値が所定値を超えるか否かを判定し、前記各々の値の何れもが前記所定値を超えていると判定した場合、前記第1のモードを選択する
請求項1に記載のデータ処理装置。 - 前記モード選択部は、前記第1の光強度分布データから得られる前記値が前記所定値を超え、前記第2の光強度分布データから得られる前記値が前記所定値を超えていないと判定した場合、前記第2のモードを選択する
請求項5に記載のデータ処理装置。 - 前記所定値は、光強度に対する値である
請求項5に記載のデータ処理装置。 - 前記所定値は、時間又は距離に対する値である
請求項5に記載のデータ処理装置。 - 前記第1の光強度分布データ及び第2の光強度分布データは、前記検出対象が前記収容領域に収容されていない状態で取得されたデータである
請求項1に記載のデータ処理装置。 - 前記モード選択部は、前記第1の光強度分布データ及び前記第2の光強度分布データから得られる各々の値が所定値に満たないか否かを判定し、前記各々の値の何れもが前記所定値に満たないと判定した場合、前記第1のモードを選択する
請求項9に記載のデータ処理装置。 - 前記モード選択部は、前記第1の光強度分布データから得られる前記値が前記所定値に満たず、前記第2の光強度分布データから得られる前記値が前記所定値以上であると判定した場合、前記第2のモードを選択する
請求項10に記載のデータ処理装置。 - 前記データ判定部は、前記第2のモードにおいて、前記第1の光強度分布データにおける前記解析対象範囲の位置に対する、前記第2の光強度分布データにおける前記解析対象範囲の位置に関する補正情報に基づき、前記第2の光強度分布データの前記解析対象範囲を特定する
請求項2に記載のデータ処理装置。 - 前記補正情報は、前記第1の光源及び前記第2の光源の間で予め定められた固定値である
請求項12に記載のデータ処理装置。 - 前記固定値は、前記第1の光源と第2の光源の間の距離、及び前記相対位置が変化する速度に基づく
請求項13に記載のデータ処理装置。 - 前記固定値は、前記第1の光源及び前記第2の光源の各々が所定の地点を通過する時刻の差に基づく
請求項13に記載のデータ処理装置。 - 検出領域に向けて光を出射する第1の光源及び第2の光源と、
前記第1の光源から前記検出領域に向けて出射された光に基づいて得られる第1の光強度分布データと前記第2の光源から前記検出領域に向けて出射された光に基づいて得られる第2の光強度分布データの各々について、検出対象が収容される収容領域に対応する解析対象範囲を特定するデータ判定部と、
該データ判定部の動作モードを選択するモード選択部と、を有するデータ処理装置と、を備え、
該モード選択部は、前記データ判定部が、前記第1の光強度分布データ及び前記第2の光強度分布データの各々から、前記解析対象範囲を特定する第1のモードと、
前記データ判定部が、前記第1の光強度分布データの前記解析対象範囲に関する情報に基づき、前記第2の光強度分布データから前記解析対象範囲を特定する第2のモードの、何れか一方を選択する
光学検出システム。 - 前記第1の光源及び前記第2の光源は、一のユニットに備えられ、一体で移動可能に構成されている
請求項16に記載の光学検出システム。 - 前記第1の光源及び前記第2の光源の各々は、複数のユニットに配設され、該ユニットは、互いに独立して移動可能に構成されている
請求項16に記載の光学検出システム。 - データ判定部が、第1の光源から検出領域に向けて出射された光に基づいて得られる第1の光強度分布データと第2の光源から前記検出領域に向けて出射された光に基づいて得られる第2の光強度分布データの各々について、検出対象が収容される収容領域に対応する解析対象範囲を特定することを含み、
モード選択部は、前記データ判定部が、前記第1の光強度分布データ及び第2の光強度分布データの各々から、前記解析対象範囲を特定する第1のモードと、
前記データ判定部が、前記第1の光強度分布データの前記解析対象範囲に関する情報に基づき、前記第2の光強度分布データから前記解析対象範囲を特定する第2のモードの、何れか一方を選択する
データ処理方法。 - データ判定部が、第1の光源から検出領域に向けて出射された光に基づいて得られる第1の光強度分布データと第2の光源から前記検出領域に向けて出射された光に基づいて得られる第2の光強度分布データの各々について、検出対象が収容される収容領域に対応する解析対象範囲を特定する機能を含み、
モード選択部は、前記データ判定部が、前記第1の光強度分布データ及び第2の光強度分布データの各々から、前記解析対象範囲を特定する第1のモードと、
前記データ判定部が、前記第1の光強度分布データの前記解析対象範囲に関する情報に基づき、前記第2の光強度分布データから前記解析対象範囲を特定する第2のモードの、何れか一方を選択する機能を
コンピュータに実現させるためのデータ処理プログラム。
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