WO2010121465A1 - 诱导多能性干细胞快速高效产生的新型无血清培养基以及使用其的方法 - Google Patents

诱导多能性干细胞快速高效产生的新型无血清培养基以及使用其的方法 Download PDF

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WO2010121465A1
WO2010121465A1 PCT/CN2009/074358 CN2009074358W WO2010121465A1 WO 2010121465 A1 WO2010121465 A1 WO 2010121465A1 CN 2009074358 W CN2009074358 W CN 2009074358W WO 2010121465 A1 WO2010121465 A1 WO 2010121465A1
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medium
cells
cell
ips
concentration
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裴端卿
陈捷凯
刘晶
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中国科学院广州生物医药与健康研究院
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Priority to DK09843569.6T priority Critical patent/DK2423302T3/en
Priority to ES09843569.6T priority patent/ES2548883T3/es
Priority to US13/265,553 priority patent/US20120100568A1/en
Priority to JP2012506306A priority patent/JP2012524525A/ja
Priority to EP09843569.6A priority patent/EP2423302B8/en
Publication of WO2010121465A1 publication Critical patent/WO2010121465A1/zh

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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0696Artificially induced pluripotent stem cells, e.g. iPS
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    • C12N2500/00Specific components of cell culture medium
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20Cytokines; Chemokines
    • C12N2501/23Interleukins [IL]
    • C12N2501/235Leukemia inhibitory factor [LIF]

Definitions

  • Novel serum-free medium for inducing rapid and efficient production of pluripotent stem cells and methods for using same
  • the present invention relates to a rapid and efficient reprogramming of somatic cells into pluripotent stem cells
  • the present invention relates to the use of said medium for inducing pluripotent stem cells, and a method for screening compounds, especially high-throughput screening of compounds.
  • Stem cells are the initial source of the human body and its various tissue cells. Its most prominent biological characteristics are its ability to self-renew and proliferate, as well as the potential for multi-directional differentiation. Stem cells are classified into adult stem cells (emmatic stem cells) and embryonic stem cells (ES cells) according to different sources.
  • adult stem cells include those found in adult tissues such as bone marrow mesenchymal stem cells, pancreatic stem cells, and neural stem cells.
  • hES cells are used as seed cells, which can provide a large amount of materials for clinical transplantation of cells, tissues or organs.
  • Specific tissue cell types can be obtained by controlling the differentiation environment of hES cells and transfecting key molecular genes that can promote the differentiation of ES cells. These cells are used for transplantation and will bring new hopes for the treatment of diseases such as diabetes, Parkinson's disease, spinal cord injury, leukemia, myocardial injury, renal failure, and cirrhosis.
  • HES cell research has been faced with many problems and controversies, including the following Aspects: (1) The source of donor oocytes is difficult, and hES cells are less efficient. Furthermore, the immaturity of SCNT technology will inevitably require further consumption of human oocytes, so its source is difficult to guarantee. (2) Immunological rejection, unless the SCNT technique is used, the patient still has immune rejection in various cells and tissues differentiated from hES cells. (3) hES cells are tumorigenic and may develop into tumors after transplantation into the recipient's body. Even if SCNT technology is used, suicide genes are added to transplanted cells, etc., it may not be a good solution to this problem. (Reubinoff BE et al.
  • Embryonic stem cell lines from human blastocysts somatic differentiation in vitro. Nat Biotechnol 2000; 18: 399-404; Richards M et al, Bongso A. Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic Stem cells. Nat Biotechnol 2002; 20:933-936; Burdon T et al, cell cycle and pluripotency in embryonic stem cells. Trends Cell Biol 2002; 12: 432-438).
  • (4) Maintain hES risk in vitro Nakagawa M, et al., N, Yamanaka S. Generation of induced pluripotentstem cells without Myc from mouse and human fibroblasts. Nat Biotechnol 2008; 26: 101-106).
  • lentiviral transfection techniques may have similar risks.
  • Mouse fibroblast or adult mouse tail skin FBxl5+ pluripotent stem cell line obtained from mouse fibroblasts under the condition of mouse ES cells, which is very similar to mouse ES cells in terms of cell morphology, growth characteristics, surface markers, and formation of teratomas.
  • induced pluripotent stem cells DNA methylation and formation of chimeric animals
  • iPS cells ( Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006; 126:663-676).
  • Nanog+ iPS cell line which can form 3 germ layer tissues not only in cell morphology, growth characteristics, marker expression, but also under transplantation into mouse skin.
  • the teratoma of cell structure and the like are very similar to mouse ES cells, and are almost completely similar to mouse ES cells in terms of DNA methylation pattern, gene expression, chromatin state, formation of chimeric animals, and the like.
  • the study also found that reactivation of the proto-oncogene c-Myc is the cause of tumor formation in chimeric animals; the above four genes transfected were not expressed in iPS cells, indicating that these genes only play a role in the induction process.
  • iPS cells maintain a pluripotent state is the expression of endogenous transcription factors such as Nanog (OKita K, Ichisaka T et al, germline-competent induced pluripotent stem cells. Nature 2007; 448: 313-317).
  • endogenous transcription factors such as Nanog (OKita K, Ichisaka T et al, germline-competent induced pluripotent stem cells. Nature 2007; 448: 313-317).
  • Nanog endogenous transcription factors
  • iPS cells are derived from direct remodeling of lineage-shaped somatic cells without the discovery of reverse transcription. Integration of the virus into specific genetic loci is associated with nuclear remodeling (Aoi T et al, Generation of Pluripotent Stem Cells from Adult Mouse Liver and Stomach Cells. Science 2008).
  • iPS cells were successfully obtained.
  • Primary human fibroblast-like synovium Cells and cell lines derived from neonatal fibroblasts can also be reconstituted into iPS cells.
  • Such iPS cells are similar to hES cells in terms of cell morphology, proliferative capacity, surface antigen markers, gene expression, epigenetic status of pluripotent stem cell-specific genes, telomerase activity, etc., and when cultured in vitro and Different types of cells in three germ layers can be differentiated in teratoma formation in mice ( Takahashi K et al., Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
  • the medium used in the current iPS induction system requires serum, and in addition to batch-to-batch instability, there are a large number of undetermined components and the concentration of each component often varies greatly, making the serum have a research mechanism. A natural disadvantage. For the above reasons, applicants wish to study whether they can induce iPS with serum-free medium.
  • WO 9830679 reports a serum replacement agent (KnockOut Serum Replacement, KOSR), and a serum-free embryonic stem cell culture medium using the serum replacement agent, however, this medium cannot support the proliferation and formation of iPS.
  • KOSR KeratOut Serum Replacement
  • a general method for inducing pluripotent stem cells has an induction efficiency of about 0.01-0.5% in fetal bovine serum on feeder cells for about 14 days (see Qin, D., Li, W., Zhang, J. & Pei, D. Direct generation of ES-like cells from unmodified mouse embryonic fibroblasts by Oct4/Sox2/Myc/Klf4. Cell research 17, 959-962 (2007), Takahashi, K. & Yamanaka , S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676 (2006), Meissner, A., Wernig, M. & Jaenisch, R.
  • the present invention provides a serum-free medium capable of inducing pluripotent stem cells in a feeder-free cell condition more efficiently and rapidly than in the prior art.
  • the invention provides a serum-free medium for culturing cells comprising a base medium, a serum replacement additive, one or more tyrosine kinases, and optionally other ingredients.
  • the serum-free medium according to the present invention further comprises leukemia inhibitory factor (LIF).
  • LIF leukemia inhibitory factor
  • the present invention also relates to a method of inducing pluripotency, wherein in addition to the step of (a) - (e) above, the method further comprises introducing a reporter gene into the somatic cell, and first passing the gene before the step (c) Indicates the production of pluripotent stem cells and detects their production efficiency.
  • the invention also relates to the use of a serum-free medium according to the invention for rapid and efficient induction of somatic cell reprogramming into pluripotent stem cells.
  • the invention also relates to the use of a medium according to the invention for screening compounds, in particular high throughput screening compounds, using induced pluripotent stem cells in an iPS system.
  • Figure 1 MEF infected with 4 factors (4F, Oct4, Sox2, Klf4, and c-Myc) could not grow in mKSR medium and could not generate iPS colonies.
  • the control was cultured in serum medium using known classical methods.
  • Oct4-GFP positive cells were performed at different time points on MEF cells infected with 4 factors (4F, Oct4, Sox2, Klf4, and c-Myc) or 3 factor infection (3F, Oct4, Sox2, and Klf4) FACS analysis.
  • Serum-free medium The induced pluripotent stem cell (iPS) line obtained from iPS-SF1 is pluripotent.
  • the iPS cell line cultured by iPS-SF1 after infection has a prominent clone morphology similar to that of typical mouse stem cells, and strongly expresses Oct4-GFP indicating pluripotency.
  • the iPS cell line cultured by iPS-SF1 after infection can form a chimeric mouse after injection into the embryo, and the donor embryo is a white ICR mouse. The black hair on the mouse indicates that chimerism has occurred.
  • the iPS-SF1 culture-established iPS cell line has the ability to participate in normal development in vivo.
  • Figure 4 Percentage of Oct4-GFP positive cells measured on the 7th day after infection, as described above, with 4 factor infection, under 8 different culture conditions. Set the value of the iPS-SF1 sample to 1. The four bars on the left indicate the positive effects of the three supplements added to the iPS-SFl.
  • Mock medium was supplemented with 10% KOSR in DMEM.
  • the four bars on the right are marked separately from The negative effects of each component in iPS-SFl.
  • Serum is a strong inhibitor of reprogramming.
  • FIG. 7 Nanog promoter methylation analysis of Oct4/Sox2/Klf4 three-factor mediated iPS.
  • Three-factor fibroblasts were cultured in mES (serum-containing medium) and iPS-SF1, and samples from the second and sixth days after infection were subjected to heavy sulfate sequencing for analysis of methylation of Nanog promoter.
  • mES serum-containing medium
  • iPS-SF1 heavy sulfate sequencing for analysis of methylation of Nanog promoter.
  • chalk represents a pair of CpG nucleotides that are not methylated
  • sputum represents a pair of CpG nucleotides that are methylated.
  • Figure 8 Effect of different growth factors (including tyrosine kinase and estrogen) in reprogramming.
  • the growth factors in the figure were added to the growth factor-free iPS-SF1, respectively, and Oct4-GFP was analyzed on the 7th day after infection.
  • E2 is estrogen and FBS medium is a negative control.
  • n 2.
  • the error bar is s.d.
  • Figure 9 Representative FACS plot of infected MEF cultured in different media.
  • the signal from the PE channel was used as a control for autofluorescence.
  • iPS-SF1 significantly improved the Oct4-GFP group on the 7th day after infection in the 4F and 3 factor-infected MEF.
  • (a) cell includes plural numbers of cells, including mixtures thereof.
  • iPS cells induced pluripotent stem cells
  • basal medium refers to any medium capable of supporting cell growth.
  • the basal medium provides standard inorganic salts such as rheology, iron, magnesium, calcium, and potassium, as well as vitamins, glucose, buffer systems, and key amino acids.
  • Basal media that can be used in the present invention include, but are not limited to, Dulbecco's Modified Eagle's Medium (DMEM), Minimal Essential Medium (MEM), Basal Medium Eagle (BME), RPMI 1640, F-10, F-12, a Minimal Essential Medium In a (aMEM), Glasgow's Minimal Essential Medium (G-MEM), and Iscove's Modified Dulbecco's Medium 0 selected embodiment, the medium is a mixture of DMEM and F12 (1:1) or DMEM with high glucose. In a more preferred embodiment, the basal medium is high in glucose (eg 4.5 g/L of DMEM.
  • generation serum additive refers to an additive which is used in a cell culture to be added to a basal medium to partially or completely replace serum in supporting the growth and growth of cells, generally including insulin and a metalloproteinase. , trace elements, vitamins and other factors, these factors are generally not included in the basal medium, but are provided by serum commonly used in cultured cells.
  • the serum additive comprises at least one or more of the following components that support cell growth: one or more insulin and insulin substitutes, one or more transmetalloproteins and transmetalloprotein substitutes, one or more trace elements, one Or a plurality of vitamins, one or more amino acids, one or more hormones and hormone-like compounds, serum albumin or serum albumin substitutes, and one or more lipids and the like.
  • a variety of commercial surrogate serum additives are known, such as KonckOut Serum Replacement ⁇ KOSR), N2, B27, Insulin-Transferrin-Selenium Supplement (ITS), G5, etc., which are readily available to those skilled in the art. These additives have a well-defined composition, so the concentration of each component can be determined according to the proportion of the medium in the medium.
  • the surrogate additive used herein is a mixed additive obtained by mixing KOSR, N2 and/or B27 in a certain ratio, more preferably, the concentration of KOSR in the final medium is from 5% to 20%, and the concentration of N2 is from 0. From % to 1%, the concentration of B27 is between 0% and 2%; the most preferred solution is a concentration of 10% KOSR in the final medium and a concentration of 0.5% N2.
  • the receptor tyrosine kinase used in the present invention includes all of the receptor tyrosine kinase-like growth factors that have been identified and will be identified in the future with receptor tyrosine kinase properties.
  • the receptor tyrosine kinase is selected from the group consisting of bFGF, EGF, IGF2 or VEGF.
  • the receptor tyrosine kinase is bFGF.
  • concentration of the added receptor tyrosine kinase sufficient to maintain cell growth, depending on the type of cell to be cultured, among other conditions, preferably between about 3-20 ng/ml.
  • the culture medium of the invention may comprise about 5 ng/mL of bFGF, 10 ng/ml of EGF, 25 ng/ml of IGF2, and/or 10 ng/ml of VEGF. In a most preferred embodiment, the medium of the invention comprises Approximately 5 ng/mL of bFGF.
  • LIF refers to a leukemia inhibitory factor which is a growth factor commonly added in cultured stem cells known in the art.
  • concentration of LIF added to the medium may be between 500 U/mL and 2000 U/mL, more preferably between 700 U/mL and 1400 U/ml, and most preferably at about 1000 U/ml.
  • the serum-free medium according to the present invention is prepared by conventional techniques known to those skilled in the art, such as A LABORATORY MANUAL and ANIMAL CELL CULTURE (edited by RI Freshney, (1987)); W. French Anderson et al., HANDBOOK OF STEM Techniques and conditions for mixing serum preparation as described in CELLS.
  • embryonic cell as used herein is a concept relative to “germ cells” and “embryonic stem cells”, which are produced by “embryonic stem cells” and are no longer pluripotent, but have a specific function.
  • a cell which is produced by the differentiation of “embryonic stem cells” or the development of inner cell masses, which no longer has pluripotency, generally has specific functions, and is generally located in the embryonic stage from the developmental stage (specifically fertilized in mice) The fetal rats or adult rats after 3.5 days) are generally taken from the germ cells and their sources (such as spermatogonial stem cells, genital stem cells, etc.) that may be pluripotent.
  • the somatic cells used herein are preferably derived from a mammal, more preferably from a human, monkey, dog, cat, rat or mouse, and most preferably from a mouse.
  • the somatic cells herein may be any type of somatic cells in the body, preferably fibroblasts or meningeal cells.
  • stem cell pluripotency factor that induces somatic cell reprogramming is a factor that is critical for the maintenance of stem cell pluripotency. By introducing the factor into somatic cells, it is possible to induce somatic cell reprogramming into embryonic stem cells under certain conditions. . So far, many literatures have already smashed a number of such factors that can be used for reprogramming, see, for example, Qin, D., Li, W., Zhang, J. & Pei, D. Direct generation of ES-like cells from Unmodered mouse embryonic Fibroblasts by Oct4/Sox2/Myc/Klf4. Cell research 17, 959-962 (2007), Okita, K., Ichisaka, T.
  • said pluripotency factor comprises Oct4, Sox2 (optionally, Soxl), C-myc (optionally L-Myc or N-Myc), and Klf4 (optionally, Klf5 or Klf2), Esrrb, Nanog. and Lin28.
  • the above pluripotency factor may be based on the cell to be introduced but from any source, preferably a mouse pluripotency factor and a variant thereof, such as Sox2, NCBI accession number NM 011443.3 (mouse contains SRY-box gene 2 Oct4, NCBI accession number is NM-013633.2 (mouse POU domain, class 5, transcription factor 1 (Pou5fl)); Klf4, NCBI accession number is ⁇ _010637 ⁇ 2 (mouse Kruppel-like factor 4); c -Myc, NCBI accession number NM-010849.4 (myelocytomatosis oncogene (c-Myc)); Soxl, NCBI accession number NM-009233.3, (mouse contains SRY-box of gene 1) Klf2, NCBI accession number NM 008452.2, (mouse Kruppel-like factor 2 (lung)); Klf5, NCBI accession number ⁇ 009769.4, (mouse Kruppel-like
  • C-Myc can also be replaced by its mutant L-myc (accession number NM 008506.2, mouse v-myc myeloma virus love gene homolog lv, lung cancer-derived (avian) ((Mycll)) Or N-Myc (accession number NM-008709.3, mouse v-myc ⁇ cell tumor virus-associated oncogene, neuroblastoma-derived (avian) (Mycn).
  • L-myc accession number NM 008506.2
  • N-Myc accession number NM-008709.3
  • mouse v-myc ⁇ cell tumor virus-associated oncogene neuroblastoma-derived (avian) (Mycn).
  • induced reprogramming refers to the process of dedifferentiating somatic cells into pluripotent stem cells.
  • the somatic cell is dedifferentiated into a pluripotent stem cell by introducing a pluripotency factor cDNA required to maintain stem cell pluripotency into a somatic cell ( Takahashi K, Yamanaka S. CelL 2006; 126: 663-676; Wernig M , Meissner A, Foreman R, et al, ature. 2007; 448: 318-324; Yu J, Vodyanik MA, Smuga-Otto K et al. Science. 2007; 318: 1917-1920).
  • the pluripotency factor comprises Oct4, Sox2 (optionally, Soxl), C-myc (optionally L-Myc or N-Myc), Klf4 (optionally, Klf5 or Klf2) One or more of Nano, and Nano28.
  • the method of introducing the stem cell pluripotency factor cDNA into a somatic cell can be a variety of techniques well known to those skilled in the art, including viral infection, lipofection, transposon-mediated insertional expression, transmembrane protein, drug Various methods of transferring DNA into cells, such as induction, electroporation, and particle bombardment.
  • the transfection is carried out using a viral vector comprising cDNA, which comprises a plurality of viral vectors such as a lentiviral vector, a retroviral vector, and the like.
  • a retroviral vector e.g., a pMX vector
  • a retroviral vector is preferred, as described in the Examples.
  • condition suitable for cell growth are conventional stem cell culture conditions in the art, and include modifications, culture methods, and cultures that are suitable for each specific cell line, but do not affect the basic properties of the cells. See W. French Anderson et al. , HANDBOOK OF STEM CELLS, Volume 2.
  • the reporter gene described herein refers to a stage capable of indicating that a cell has been transformed into an embryonic stem cell by external induction, including the use of transgene or homologous recombination to join a sequence expressing a fluorescent protein or resistance, which is specific for embryonic stem cells.
  • the expression of this fluorescent protein or resistance gene can be activated when the cell reaches the state of the embryonic stem cell, so that the cell has certain traits that can be detected (such as emitting green light) ) is distinguished from other cells that have not been reprogrammed to this state.
  • Reporter genes commonly used in the art include green fluorescent proteins, resistance genes such as ampicillin resistance genes, and the like.
  • reporter genes suitable for various embodiments depending on the culture conditions and use of the cells. For example, Young II Yeom et al., Germline regulatory element of Oct-4 specific for the totipotent cycle of embryonal cells, Development 122,000-000 (1996), Printed in Great Britain, The company of Biologists Limited 1996, 881-894 Shin-ya Hatano et al., Pluripotential competence of cells associated with Nanog activation, Mechanisms of Development 122 (2005), 67-79. ⁇
  • Methods for detecting cellular pluripotency described herein are well known to those skilled in the art, see, for example, Yamanaka, S. Strategies and new developments in the generation of patient-specific pluripotent stem cells.
  • the methods include identifying expression of a pluripotency molecular marker, detection of methylation status of the cells, formation of embryo EBs, formation of teratomas, and administration of induced pluripotent stem cells to form chimeric mice and the like.
  • chimeric mice are practiced by the "chimeric mouse” technique well known to those of ordinary skill in the art. It refers to injecting embryonic stem cells or iPS cells obtained by the technique of the present invention into mouse embryos, so that they are mixed with embryonic cells injected into the embryos of the mice, and grow together in the uterus of the surrogate mothers.
  • the whole body of the whole body after the birth of the mouse is composed of two kinds of embryonic cells, such as a mosaic puzzle.
  • Such a mouse is called a chimeric mouse (Evans MJ, et al; The ability of EK cell to form chimeras after Selection of clones in G418 and some observation on the intergration of retroviral vector proviral DNA into EK cells [M]; Cold Spring Harbor Symposia on Quantitative Biology; 1985; Xian MW, Wu BY, Hu XL, Shang KG, Wu HL, 1996 Construction of chimeric mice of ES cells by microinjection method. Hereditas (Beijing) 18 (1): 7-10 (Chinese)). The ability to form chimeric mice with iPS is the most direct and critical evidence for testing whether iPS has similar properties to embryonic stem cells.
  • screening compound refers to the screening of a compound that affects the iPS process and induction by detecting the characteristics of the reporter gene or the cell itself in a given library of compounds; Known compounds that induce some to all of the factors required for the iPS process.
  • Those skilled in the art have developed methods for screening compounds using the iPS process, see, for example, Yan Shi et al, Induction of Pluripotent Stem Cells from Mouse Embryonic Fibroblasts by Oct4 and Klf4 with Small-Molecule Compounds. Cell Stem Cell 3, 568-574 , November 6, 2008.
  • Mouse embryonic fibroblasts are derived from the embryo of the Oct-GFP transgene allele and the hemizygous el3.5 of the Rosa26 allele, and cultured in the following fibroblast culture medium: high glucose DMEM supplemented with 10% FBS, L-Glutamine and NEAA. Both iPS and ES cells were cultured on MEF feeder layers in serum-containing medium (mES) and serum-free medium (mKSR). The names of the various media in this article and the components are shown in Table 1. MEF feeder cells were inactivated by mitomycin C.
  • composition culture mES mKSR FB-SF1 iPS-SFl
  • Retroviral vectors containing DNA of mouse Oct4, Sox2, Klf4 and c-Myc were purchased from Addgene. Viral production and infection according to the prior art (Qin, D., Li, W., Zhang, J. & Pei, D. Direct generation of ES-like cells from unmodified mouse embryonic fibroblasts by Oct4/Sox2/Myc/Klf4 Cell research 17, 959-962 (2007); Qin, D. et al. Mouse meningiocytes express Sox2 and yield high efficiency of chimeras after nuclear reprogramming with exogenous factors. J Biol Chem 283, 33730-33735 (2008). These plasmids were transfected into PlatE cells by conventional methods.
  • the viral supernatant was then collected and filtered for 48 hours to infect MEF supplemented with 1,5-dimercapto-1,5-diazaundecylphosphonium bromide. Repeat the same steps the next day. The day the virus supernatant was removed was defined as 0 days after infection.
  • the primary method for quantitative reprogramming efficiency is FACS analysis of Oct4-GFP positive cells. Based on the results of the timing process, infected MEF cells were trypsinized on days 7 and 9 post-infection and then analyzed by FACSCalibur. GFP-positive cells are gated by a control signal from the PE channel, with a minimum of 15,000 events recorded. Cells infected with pMXs-FLAG served as a negative control. To confirm the efficiency of the FACS analysis, GFP-positive colonies on day 14 post-infection were counted directly under a fluorescence microscope.
  • the four-factor virus was mixed in a 1:1:1:1 mixture (each 1 ml) and infected into a total of 35,000 fibroblasts in one well of a six-well plate, and at 37 degrees, 5% C0 2 was cultured in mKSR and mES medium, respectively.
  • Example 2 Application of iPS-SF1 medium can greatly improve the efficiency of inducing iPS
  • transfected cells cultured in iPS-SF1 medium can be found, and embryo-like stem cell clones with strong expression of Oct4-GFP are expressed on the 6th day after transfection.
  • the clones formed in the traditional medium did not show fluorescence (Fig. 2C).
  • the induced iPS cell line has a cell morphology similar to that of embryonic stem cells.
  • fibroblasts were infected with 3 factors, and then cultured in iPS-SF1 medium, and on the 12th to 14th day after infection, the representative clones were expressed according to the clonal morphology and fluorescence.
  • a stable iPS cell line was formed after stable passage.
  • the left panel is a normal embryonic stem cell
  • the right panel the above iPS cell line. These cell lines are very similar in morphology to embryonic stem cells and strongly express the green fluorescence of Oct4-GFP.
  • the embryos for injection were taken from four-week-old super-discharged ICR female mice (white), and caged with the same strain of male rats. The embryos were collected from the uterus and fallopian tubes when the plug was 3.5 d (0.5 d on the same day). Paraffin oil was cultured in M 16 culture droplets in a 37 C5 % C02 incubator.
  • the iPS cells (i.e., the iPS cell line obtained above) were replaced with fresh culture medium 3 h before the injection, and trypsinized to prepare a single cell suspension for use. Transfer the appropriate amount of iPS cell suspension and embryonic embryo into the M2 injection droplet, and fix the embryo with the needle holding needle under the microinjection system.
  • the IPS cells were aspirated with an injection needle, and the needle was injected from the trophoblast site far away from the inner cell mass, and 10 to 12 cells were injected into each of the sputum embryos. After the injection, the embryo cavity disappeared, and it was placed in the culture medium for 1 - 3 h in the incubator. After the embryo cavity was restored, it was transplanted into the uterus of the pseudo-pregnant rat who was seen for 2.5 d.
  • Figure 3-B shows chimeric mice constructed using the iPS obtained by the method of the present invention, wherein the non-chimeric mice are pure white, and the chimeric mice are derived from the injected OG2/Rosa26 mice because the injected iPS cells are derived from black.
  • the black and white body color distribution suggests that the iPS cells obtained by the method of the present invention can participate in normal in vivo development and form chimeric mice, suggesting that these cells are not different in development from embryonic stem cells.
  • Example 4 Acceleration of the ubiquitin gene promoter dethiolation in the iPS induction process of the present invention
  • Oct4 in the cultured medium and iPS-SFl mES medium of the present invention Sox2, Klf4 infection three-factor (prepared as described above) obtaining a cell line genomic DNA (700n g), by exposing it to a 50.6% A mixture of sodium hydrogen sulfite (Sigma S-1516) and 10 mM hydroquinone (Sigma H-9003) was used overnight for bisulfite modification.
  • the promoter region of Nanog was amplified by PCR using the primer set described in Table 2. The PCR product was cloned into a flat pMD8-T vector ( Takara), propagated in DH5a, and sequenced.
  • the pluripotency gene promoter is dethiolated.
  • MEF was infected with Oct1, Sox2, Klf4 virus 1:1:1, and cultured in traditional mES medium and in the medium iPS-SF1 of the present invention, collected 2 days and 6 days after infection.
  • Nanog was amplified and sequenced by a sequencing company known in the art (for example, Invitrogen), and the sequencing results were used to analyze the thiolation state of the Nanog promoter during iPS.
  • the MEF after 3-factor infection slowly demethylation of the Nanog promoter in DES (D2: 33%, D6: 41.7 %), while in the medium of the invention (iPS-SFl) In the case of D2, it has reached 66.7 % demethylation state and continues. At D6, the demethylation level has reached 80.9 %, indicating that the fragment detected in the Nanog promoter has been Almost activated, and the activation of Nanog, a typical stem cell pluripotency factor, signals the induction of iPS.
  • the iPS-SF1 medium of the present invention can not only successfully induce reprogramming of iPS, and the induced reprogramming can avoid infection by using the carcinogenic c-Myc gene, and the efficiency in reprogramming.
  • the rate of demethylation is much higher than the existing mES medium.
  • Example 5 Effect of components in the medium of the invention on iPS reprogramming efficiency
  • the LIF, N2 and bFGF in the iPS-SF1 medium listed in Table 1 were respectively deleted, or in DMEM + 10% KOSR (ie, without growth factors, and containing NEAA, L-Glutamine, etc., hereinafter referred to as
  • the effects of LIF, N2, and bFGF on the iPS reprogramming efficiency of MEF cells transfected with 4 factors were measured by adding N2 and bFGF respectively to the concentrations corresponding to iPS-SF1 in bKSR) medium.
  • the meaning of each mark in Figure 4 is as follows:
  • iPS-SFl—LIF iPS-SFl without LIF
  • iPS-SFl -N2 no N2 iPS-SFl
  • iPS-SFl -bFGF iPS-SFl without bFGF
  • Example 6 Inhibition of serum on iPS reprogramming cultured in iPS-SF1 medium As shown in Fig. 5, when 2%, 4%, 6%, 8%, and 10% of serum were added to the iPS-SF1 medium, respectively, the serum reprogrammed the iPS unlike the known prior art. The efficiency is suppressed up to 96%.
  • Example 7 The effect of other tyrosine kinases on the medium of the present invention leads to the iPS reprogramming process.
  • the blank control is iPS-SF1 without bFGF.
  • the experimental group added various receptor tyrosine kinases (including basic fibroblast growth factor bFGF, epidermal growth factor EGF, vascular endothelial growth factor VEGF) in blank medium (ie iPS-SF1 without bFGF). Insulin-like growth factor II IGF2, and androdiol, serum medium (FBS) were used as negative controls for this experiment. As shown in Figure 8, in addition to bFGF, IGF2, VEGF, and estrogen were induced for the iPS reprogramming process.
  • Equivalent effect Example 8 Screening compounds during iPS in iPS-SF1 medium
  • 20,000 MEF cells were seeded in each well of a 12-well plate, the medium was iPS-SF1, and then virus infection was carried out as described above to transfect 4 factors.
  • the various compounds as shown in Figure 6 were separately added to the medium at the following concentrations: ⁇ 0325901 (1 ⁇ ), CHIR99021 (3 M), ⁇ 5402 (2 ⁇ , EMD biosciences), ⁇ -27632 (10 ⁇ ), vitamin ⁇ (25 ⁇ , Sigma), vitamin ⁇ (1 ⁇ , Sigma), ⁇ 83-01 (0.5 ⁇ , EMD), 8 ⁇ 203580 (2 ⁇ ),
  • VPA (lmM, EMD), JAK inhibitor (0.3 ⁇ , EMD), 5aza-DC (l M, Sigma),
  • TSA and VPA can significantly enhance the efficiency of iPS reprogramming (100%).
  • a compound which is advantageous for self-renewal of mouse embryonic stem cells is reported, for example.
  • SU5402 reduces the efficiency of iPS reprogramming.

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Description

诱导多能性干细胞快速高效产生的新型无血清培养基以及使用其的方 法 一. 技术领域
本发明涉及一种能够快速有效地将体细胞诱导重编程为多能性干细胞
( induced Pluripotent stem cell, iPS )的无血清培养基, 以及使用该无血清 培养基在无需饲养层 ( feeder )的条件下诱导重编程体细胞的方法, 其中 所有诱导重编程的速度以及效率均大大提高。 此外, 本发明还涉及所述培 养基用于诱导多能性干细胞的用途, 以及用于篩选化合物, 尤其是化合物 的高通量筛选的方法 二. 发明背景
干细胞 (stem cells)是人体及其各种组织细胞的初始来源,其最显著的 生物学特征是既有自我更新和不断增殖的能力, 又有多向分化的潜能。 干 细胞根据不同的来源分为成体干细胞 (somaticstem cells)和胚胎干细胞 (embryonic stem cells,ES细胞)。 成体干细胞包括骨髓间充质干细胞、 胰 腺干细胞、 神经干细胞等成体组织中存在的。
1981年, ES细胞的分离和培养首先在小鼠中获得成功,是至今研究最 广泛、 最成熟的干细胞体系。 随后,牛、 羊等大动物的 ES细胞分离和培 相继获得成功。
hES 细胞研究的应用前景主要是移植治疗,在组织工程学领域中以 hES 细胞作为种子细胞,可为临床上细胞、 组织或器官的移植治疗提供大 量的材料。 通过控制 hES细胞分化培养环境、转染能够促进 ES细胞定向 分化的关键分子基因等体外诱导分化策略,可获得特异性的组织细胞类 型。 这类细胞用于移植治疗,将给糖尿病、 帕金森氏病、 脊髓损伤、 白血 病、 心肌损伤、 肾衰竭、 肝硬化等疾病的治疗带来新的希望。
一直以来, hES 细胞研究面临着许多难题和争议,主要包括以下几个 方面:(1)供体***的来源困难, hES细胞建系效率低。 此夕卜, SCNT技 术的不成熟必将需要进一步耗费更多的人类***,故而其来源难以得 到保证。 (2)免疫排斥反应,除非采用 SCNT技术,否则患者对 hES细胞分 化而来的各种细胞和组织仍然存在免疫排斥反应。(3)hES细胞具有成瘤性, 移植到受体的体内后有发展为肿瘤的可能性, 即使采用 SCNT技术、 给移 植细胞设置***基因等应对措施, 也不一定能够很好地解决这个问题 ( Reubinoff BE等人. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro。 Nat Biotechnol 2000; 18: 399-404; Richards M 等人, Bongso A. Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol 2002; 20:933-936; Burdon T等人, cell cycle and pluripotency in embryonic stem cells. Trends Cell Biol 2002; 12: 432-438 )。 (4)体外保持 hES风险 (Nakagawa M,等人, N, Yamanaka S. Generation of induced pluripotentstem cells without Myc from mouse and human fibroblasts. Nat Biotechnol 2008; 26: 101-106)。 同样,慢病毒转染技术可 能也存在类似的风险。
为避开 hES细胞和治疗性克隆研究的伦理学争论,需要找到一种替代 途径,以便将人类的体细胞直接转化为多潜能干细胞,为患者提供 "个性 化" 的自体干细胞。 2003年, Gurdon研究小组发现,将已完全分化的小 物细胞核的分化标志物丧失, 而哺乳动物干细胞中最具特征性的标志物 Oct4则呈高表达,提示哺乳动物细胞核可直接被两栖动物***核泡所 重构从而表达 Oct4 ( Byrne JA等人, uclei of adult mammalian somatic cells are directly reprogrammed to oct-4 stem cell gene expression by amphibian oocytes. Curr Biol 2003;13: 1206-1213 ) 。
2006年, 日本京都大学 Yamanaka研究小组采用体外基因转染技术, 从 24个因子中筛选出 Oct4、 Sox2、 c-Myc、 Klf4等 4个转录因子,通过逆 转录病毒将上述 4个转录因子导入胚胎小鼠成纤维细胞或成年小鼠尾部皮 肤成纤维细胞,在小鼠 ES细胞的培养条件下获得了 Fbxl5+的多潜能干细 胞系,该细胞系在细胞形态、 生长特性、 表面 标志物、 形成畸胎瘤等方面 与小鼠 ES细胞非常相似, 而在基因表达讲、 DNA甲基化方式及形 成嵌合 体动物方面却不同于小鼠 ES细胞,故将其命名为诱导的多能性干细胞
( iPS细胞 ) ( Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006; 126:663-676 ) 。
2007-07, Yamanaka研究小组进一步用 Nanog代替 Fbxl5进行筛选, 得到了 Nanog+的 iPS 细胞系,该 iPS细胞不仅在细胞形态、生长特性、标 志物表达、移植到小鼠皮下可形成包含 3个胚层组织细胞结构的畸胎瘤等 方面与小鼠 ES细胞非常相似, 而且在 DNA甲基化方式、基因表达傅、染 色质状态、 形成嵌合体动物等方面也与小鼠 ES细胞几乎完全相似。 此外, 研究还发现重新激活原癌基因 c-Myc是嵌合体动物出现肿瘤形成的原因; 而转染的上述 4个基因在 iPS细胞中并没有表达,表明这些基因只在诱导 过程中起作用, iPS细胞保持多潜能性状态的原因是内源性转录因子, 如 Nanog等基因的表达 ( Okita K, Ichisaka T等人, germline-competent induced pluripotent stem cells. Nature 2007; 448: 313-317 )。 同时独立发 表的另一篇来自美国科学家的研究论文同样证实了上述 4个转录因子足以 使小鼠成纤维细胞在体外诱导重构成为类似小鼠 ES细胞的 iPS细胞
( Wernig M等人, In vitro reprogramming of fibroblasts into a
pluripotent ES cell-like state. Nature 2007; 448: 318-324 ) 。
新近报道了小鼠肝细胞和胃上皮细胞同样也可被重构成为 iPS细胞, 遗传学细胞讲系示踪分析显示, iPS细胞源自谱系定型的体细胞的直接重构, 而未发现逆转录病毒整合到特定的基因位点与细胞核重构相关 (Aoi T等 人, Generation of Pluripotent Stem Cells from Adult Mouse Liver and Stomach Cells. Science 2008)。
还有研究者利用相同的技术,将上述同样的 4个转录因子导入到人类 皮肤成纤维细胞中,也成功获得了 iPS细胞。 原代人类成纤维细胞样滑膜 细胞和源自新生儿成纤维细胞的细胞系同样也可被重构成为 iPS细胞。 这 类 iPS细胞在细胞形态、 增殖能力、 表面抗原标志、 基因表达讲、 多潜能 干细胞特异性基因的 表观遗传学状态、 端粒酶活性等方面与 hES细胞相 似,并且在体外培养时和在小鼠体内畸胎瘤形成中均可分化为 3个胚层的 不同细胞类型 ( Takahashi K等人, Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007; 131: 861-872 )。 与 此同时,威斯康辛大学 Thomson研究小组也报道了成功诱导胎儿成纤维细 胞转化为具有 hES细胞基^ 征的人类 iPS细胞,所不同的是他们使用慢 病毒作为载体,并在 14个候选基因中选择 了 Oct4、 Sox2、 Nanog、 Lin28 等 4个基因进行转导( Yu J等人, Induced pluripotent stem cell lines derived from human somatic cells. Science 2007; 318: 1917-1920 ) 。
Park IH等人, Reprogramming of human somatic cells to
pluripotency with defined factors. Nature 2008; 451: 141-146利用来自胎 儿、 新生儿及成人的皮肤或肺部的原代成纤维细胞,其中包括来自 1名健 康男性皮肤活检得到的成纤维细胞,采用 Yamanaka研究小组的策略也获 得了相同的结果。他们还发现 Oct4和 Sox2在诱导重构为 iPS 细胞过程中 是必需的,正是这两个转录因子维持了人类 iPS细胞的多潜能性, 而 Klf4 和 c-Myc的 作用是改变染色质的结构,从而有利于 Oct4和 Sox2的结合, 以提高诱导的效率。 此夕卜,这项研究的重要意义在于将取自皮肤活检的成 纤维细胞诱导为 iPS细胞。 上述研究表明,从活检人类皮肤组织中提取体 细胞后进行诱导以制备患者特异性的干细胞是可行的, 因而有望克服细胞 移植治疗中存在的免疫排斥反应。 鉴于导入 c-Myc基因可使嵌合体小鼠 的肿瘤发生率高达 20%,可能会阻碍其未来的临床应用( Okita K, Ichisaka T, Yamanaka S. Generation of germline-competent induced pluripotent stem cells. Nature 2007; 448: 313-317 )。 因此, Yamanaka研究小组新近才艮 it, 小鼠和人类皮肤成纤维细胞转染 c-Myc以外的其余 3个基因,在调整 培养奈件后也可得到 iPS细胞。 去除 c-Myc基因尽管可使未来临床应用的 安全性显著提高,但形成 iPS细胞的效率却明显降低。 ( Nakagawa M,等 人, Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat Biotechnol 2008; 26: 101-106 )。
然而, 尽管已经开发了大量涉及 iPS的方法, 但是由于 iPS目前存在 病毒作为基因载体, 效率低, 使用癌基因 C - Myc等问题, 最理想的 方案是通过药物诱导体细胞直接变化成为 iPS, 在化学成份确定的培养基 中进行该过程和相继的分化过程,从而得到完全安全的治疗用细胞。然而, 仅仅通过现有的知识来预测某种药物能够取代某一因子是不可能的, 最佳 方法是高通量筛选, 高通量筛选需要高效稳定的 iPS诱导***, 高效是为 了减少孔径, 在同样成本下提高通量; 稳定是为了消除批次之间差异, 如 果筛选上百万种药物, 可重复性是艮重要的。
然而, 现在的 iPS诱导***中所用的培养基均需要血清, 而血清除了 批次间不稳定外, 还存在大量无法确定的成份且各个成分浓度经常有大幅 度变化, 使得血清在研究机理方面有着天生的劣势。 鉴于以上原因, 申请 人希望研究是否能用无血清培养基诱导 iPS。
WO9830679报道了一种血清替代剂( KnockOut Serum Replacement, KOSR ) , 以及使用该血清替代剂的无血清胚胎干细胞培养基, 但是, 这 种培养基无法支持 iPS的增殖和形成。
迄今为止, 尚未有文献报道可以在小鼠体细胞, 特别是容易取材的成 纤维细胞中全程无血清诱导 iPSe
此外, 在现有的技术中, 一般的诱导多能性干细胞的方法在胎牛血清 中在饲养层细胞上在大约 14天内, 诱导效率为大约 0.01-0.5% (参见 Qin, D., Li, W., Zhang, J. & Pei, D. Direct generation of ES-like cells from unmodified mouse embryonic fibroblasts by Oct4/Sox2/Myc/Klf4. Cell research 17, 959-962 (2007)、 Takahashi, K. & Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676 (2006)、 Meissner, A., Wernig, M. & Jaenisch, R. Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells. Nature biotechnology 25, 1177-1181 (2007)、 Takahashi, K.等人 Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-872 (2007)、 Yamanaka, S. Strategies and new developments in the generation of atient-specific pluripotent stem cells. Cell Stem Cell 1, 39-49 (2007). )
因此, 本发明提供了可以比现有技术更高效快速的在无饲养层细胞的 条件下诱导多能性干细胞的无血清培养基。 三. 发明内容
在第一个方面中, 本发明提供了培养细胞的无血清培养基, 其包括基 础培养基、代血清添加剂、一种或多种酪氨酸激酶, 以及任选地其它成分。
另外, 根据本发明的无血清培养基还包含白血病抑制因子(LIF )。 在本发明的另一个方面中, 提供了使用上述无血清培养 体细胞高 效率诱导多能性干细胞的方法, 其包括以下步骤:
( a ) 将一个或多个干细胞多能性因子导入体细胞;
( b ) 用本申请的无血清培养基, 在适合于细胞生长的条件下, 培 养(a ) 中经导入的体细胞, 以将体细胞诱导重编程为多能性 干细胞;
( c ) 检测并且分析经诱导的细胞的多能性;
( d ) 挑出具有多能性的经诱导的多能性干细胞的单克隆;
( e ) 在适于胚胎干细胞生长的条件下培养( d ) 中的单克隆细胞。 本发明还涉及诱导多能性的方法, 其中除了包含上述(a ) - ( e )的步 骤外, 还另外包括将报告基因导入体细胞, 并且在步骤( c )之前首先通过 才艮告基因来指示多能性干细胞的产生以及检测其产生效率。
在本发明的另一个方面中, 本发明还涉及根据本发明的无血清培养基 快速高效诱导体细胞重编程为多能性干细胞的用途。
最后, 本发明还涉及根据本发明的培养基用于在 iPS***中使用经诱 导的多能性干细胞筛选化合物, 尤其是高通量筛选化合物的用途。 四. 附图说明
图 1: 用 4个因子感染(4F, Oct4、 Sox2、 Klf4和 c-Myc )的 MEF 不能在 mKSR培养基中生长, 且不能生成 iPS集落, 对照为用已知的经典 方法培养在血清培养基( mES ) 中的 iPS细胞。 标尺, lOOuM
图 2: 无血清 iPS-SFl比有血清的干细胞培养基能更好地支持 iPS的 形成。
a. MEF在 FBS培养基、 iPS-SFl培养基中的生长曲线( n=3 ) 。 b. 在用 4因子感染(4F, Oct4、 Sox2、 Klf4和 c-Myc )或 3因 子感染(3F, Oct4、 Sox2和 Klf4 )的 MEF细胞上在不同的时间点上 进行 Oct4-GFP阳性细胞的 FACS分析。被感染的细胞分别培养在 mES 培养基和 iPS-SFl培养基中。 N=2, 误差条指示为 s.d。
c. 用 4 因子感染的 MEF 细胞分别培养在 mES培养基以及 iPS-SFl培养基中, 六天后可见指示细胞重编程至多能性的 Oct4-GFP 仅在 iPS-SFl所培养的细胞中表达,并且形成克隆形态,而培养在经典 的血清培养基 mES则不表达 Oct4-GFP。 标尺, lOOuM
图 3.无血清培养基 iPS-SFl得到的诱导多能性干细胞 ( iPS )系具有多 能性。
a. 感染后由 iPS-SFl培养得到的 iPS细胞系具有与典型小鼠干 细胞相似的突起克隆形态, 并强烈表达指示多能性的 Oct4-GFP。
b. 感染后由 iPS-SFl培养得到的 iPS细胞系可以在注射到嚢胚 后形成嵌合体小鼠,供体嚢胚为白色 ICR小鼠, 小鼠身上的 黑色毛发提示已经发生嵌合, 证明 iPS-SFl培养建立的 iPS 细胞系具有参与体内正常发育的能力。
图 4.在 8种不同的培养条件下, 在感染后第 7天, 如上所述, 用 4因 子感染时测量 Oct4-GFP阳性细胞的百分比。将 iPS-SFl样品的值设定 为 1。 左边四条指示了 3种补充物分别添加到 iPS-SFl中的积极作用。
Mock培养基时补充有 10%KOSR的 DMEM。 右边 4条标明分别从 iPS-SFl中消除每种组分后的消极作用。
图 5:血清是重编程的强抑制剂。对于用 4因子感染的 MEF细胞在感 染后笫 7天分析 Oct4-GFP阳性细胞的百分比。 将血清以不同浓度补充到 iPS-SFl中。 N=2, 误差条指示为 s.d。
图 6: iPS-SFl作为筛选工具。 选择了常见^ ff号通路的靶向化合物。 虚线显示了其值被设定为 1的对照样品的值。 n=2, 误差条指示为 s.d。
图 7: Oct4/Sox2/Klf4三因子介导的 iPS过程中的 Nanog启动子甲基 化分析。 感染了三因子的成纤维细胞分别培养在 mES (含血清培养基)及 iPS-SFl 中, 取感染后第二天和第六天的样品进行重硫酸盐测序法分析 Nanog启动子的甲基化状态, 白圏代表未被甲基化的 CpG核苷酸对,而黑 圏表示被甲基化的 CpG核苷酸对。
图 8: 不同生长因子(包括酪氨酸激酶和*** )在重编程中的效果。 分别向无生长因子的 iPS-SFl中添加图中的生长因子, 并且在感染后第 7 天分析 Oct4-GFP。其中 E2是***, FBS培养基作用为阴性对照。 n=2。 误差条为 s.d.
图 9: 培养在不同培养基中的感染的 MEF的代表性 FACS图。 来自 PE通道的信号被用作为自发荧光的对照。其中 iPS-SFl在 4因子和 3因子 感染的 MEF中在感染后第 7天均显著改善了 Oct4-GFP群。 具体实施方案
定义和技术
除非另外指明, 本发明的实践将使用分子生物学、 微生物学、 细胞生 物学、 免疫学和重组 DNA的传统技术, 其属于本领域技术范围。 参见例 如, Sambrook, Fritsch和 Maniatis,分子克隆实驗指南, 笫 3版(2002); CURRENT PROTOCOLS IN MOLECULAR BIOLOGY (F. M. Ausubel 等人编著 (1987)); 丛书 METHODS IN ENZYMOLOGY (Academic Press, Inc.): PCR 2: A PRACTICAL APPROACH (M.J. MacPherson, B.D. Hames 和 G.R. Taylor编著, (1995)), Harlow和 Lane 编著, (1988) ANTIBODIES , A LABORATORY MANUAL and ANIMAL CELL CULTURE (R.I. Freshne 编著, (1987)); W. French Anderson等人, HANDBOOK OF STEM CELLS, 卷 2。
除非另外说明, 本文中所用的术语均具有本领域技术人员常规理解的 含义, 为了便于理解本发明, 将本文中使用的一些术语进行了下述定义。
在说明书和权利要求书中使用的, 单数型 "一个", 和"这个"包括复数 参考, 除非上下文另有清楚的表述。 例如, 术语"(一个)细胞,,包括复数 的细胞, 包括其混合物。
所有的数字标识, 例如 pH、 温度、 时间、 浓度和分子量, 包括范围, 都是近似值。 要了解, 虽然不总是明确的叙述所有的数字标识之前都加上 术语"约,,。 也要了解, 虽然不总是明确的叙述, 本文中描述的试剂仅仅是 示例, 其等价物是本领域已知的。
本文所述的 "诱导的多能性干细胞(iPS 细胞) " 是这样的细胞, 其 来源是体细胞通过诱导体细胞重编程的干细胞多能性因子体外诱导变化而 成, 其在 ES细胞培养条件下, 与 ES细胞在细胞形态、 生长特性、表面标 志物表达、 移植到皮下可形成包含 3个胚层组织细胞结构的畸胎瘤等方面 与小鼠 ES细胞非常相似, 而且在 DNA甲基化方式、基因表达借、 染色质 状态、 形成嵌合体动物等方面也与小鼠 ES细胞几乎完全相似。
本发明所用的术语 "基础培养基" 是指任何能够支持细胞生长的培养 基。 基础培养基提供了标准的无机盐例如辞、 铁、 镁、 钙、 和钾, 以及维 生素、 葡萄糖、 緩冲***以及关鍵的氨基酸。 可以用于本发明的基础培养 基包括但不限于 Dulbecco's Modified Eagle's Medium ( DMEM )、 Minimal Essential Medium ( MEM )、 Basal Medium Eagle (BME), RPMI 1640、 F-10、 F-12、 a Minimal Essential Medium ( aMEM )、 Glasgow's Minimal Essential Medium (G-MEM)、 以及 Iscove's Modified Dulbecco's Medium 0 选的实施方案中, 培养基是 DMEM与 F12的混合物(1:1 )或高葡萄 糖的 DMEM。在更优选的实施方案中,基础培养基是具有高葡萄糖(例如 4.5g/L )的 DMEM。
本发明所用的术语 "代血清添加剂" 是指在细胞培养中, 用于加入到 基础培养基中, 以部分或全部替代血清在支持细胞生存生长作用方面作用 的添加剂, 一般包括胰岛素、 转金属蛋白、 微量元素、 维生素等因子, 这 些因子一般并不包含于基础培养基中, 而是由通常培养细胞使用的血清所 提供。 代血清添加剂包含支持细胞生长的至少一种或多种以下组分: 一个 或多个胰岛素及胰岛素替代物、 一个或多个转金属蛋白及转金属蛋白替代 物、 一个或多个微量元素、 一个或多个维生素、 一个或多个氨基酸、 一个 或多个激素及类激素化合物、 血清白蛋白或血清白蛋白替代物、 以及一个 或多个脂类等。 已知多种商业化的代血清添加剂, 例如 KonckOut Serum Replacement^ KOSR ), N2, B27, Insulin-Transferrin-Selenium Supplement (ITS), G5等, 这是本领域技术人员容易获得的。 这些添加剂具有成份明 确的特点, 故而可以按照其在培养基中所占比例确定其各组分的浓度。
本领域技术人员可以根据现有技术, 根据所要培养的细胞类型以及其 它方面容易地配置代血清添加剂。 优选地, 本文中所用的代血清添加剂由 KOSR、 N2和 /或 B27按一定比例混合得到的混合添加剂, 更优选地, 最 终培养基中 KOSR的浓度从 5 % - 20 % , N2的浓度从 0 %到 1 %, B27的 浓度在 0 % - 2 %之间; 最优选的方案是在最终培养基中 KOSR的浓度为 10 %, N2的浓度为 0.5 %。
本发明所用的受体酪氣酸激酶包括所有的已经鉴定出的以及将来即将 鉴定出的具有受体酪氨酸激酶性质的所有受体酪氨酸激酶类生长因子。 优 选地, 受体酪氨酸激酶选自 bFGF、 EGF、 IGF2或 VEGF。 最优选地, 受 体酪氨酸激酶为 bFGF。 本领域技术人员可以根据所要培养的细胞种类以 及其它条件容易地确定所添加的受体酪氨酸激酶的浓度, 使之足以维持细 胞生长, 所述浓度优选在大约 3-20ng/ml之间, 更优选在大约 5-15ng/ml 之间,最优选在于大约 7ng/mL。在一个优选的实施方案中,本发明的培养 基中可以包含大约 5ng/mL的 bFGF、 lOng/ml的 EGF、 25ng/ml的 IGF2, 和 /或 10ng/ml的 VEGF。 在最优选的实施方案中, 本发明的培养基中包含 大约 5ng/mL的 bFGF。
本文所用的 LIF是指白血病抑制因子, 它是本领域已知的培养干细胞 中通常添加的生长因子。 本领域技术人员能够根据具体条件调节具体培养 基中所用到的 LIF的浓度。在本发明的培养基中,优选培养基中添加的 LIF 的浓度可以在 500U/mL - 2000U/mL之间, 更优选 700U/mL-1400U/ml之 间, 最优选在于大约 1000U/ml。
本领域的技术人员已知为了能够有利于细胞的生长, 任选地还可以在 培养基中添加其它组分。 本领域技术人员已知根据所培养的细胞以及其它 来选择需要在具体培养基中添加的其它组分,例如 L-谷氨酰胺, EAA MEM, 以及 2-疏基乙醇等。
根据本发明的无血清培养基是通过本领域技术人员已知的常规技术制 备的, 如 A LABORATORY MANUAL and ANIMAL CELL CULTURE (R.I. Freshney编著,(1987)); W. French Anderson等人, HANDBOOK OF STEM CELLS中所述的混合配制血清的技术和条件。
本文中所用的术语 "体细胞"是相对于 "生殖细胞" 以及 "胚胎干细 胞" 而言的概念, 其是由 "胚胎干细胞" 分化产生的不再具有多能性, 而 是具有某一具体功能的细胞, 其是由 "胚胎干细胞" 分化或内细胞团继续 发育产生的不再具备多能性, 一般具有具体功能的细胞, 其一般从发育阶 段位于嚢胚期(在小鼠中具体为受精后 3.5天)之后的胎鼠或成鼠取材, 取材时一般避免取到可能具有多能性的生殖细胞及其来源(如精原干细胞、 生殖嵴干细胞等)。 本文中所用的体细胞优选来源于哺乳动物, 更优选来 源于人、 猴、 狗、 猫、 大鼠或小鼠, 最优选地, 来源于小鼠。 本文中的体 细胞可以是机体内任何类型的体细胞, 优选为成纤维细胞或脑膜细胞。
本文所述的 "诱导体细胞重编程的干细胞多能性因子" 为对于干细胞 多能性维持关键的因子, 通过向体细胞中导入所述因子可以在一定条件下 诱导体细胞重编程为胚胎干细胞。 迄今为止众多文献已经才艮道了多个可以 用于重编程的这样的因子, 参见例如 Qin, D., Li, W., Zhang, J. & Pei, D. Direct generation of ES-like cells from unmodiiled mouse embryonic fibroblasts by Oct4/Sox2/Myc/Klf4. Cell research 17, 959-962 (2007)、 Okita, K., Ichisaka, T. & Yamanaka, S. Generation of germline-competent induced pluripotent stem cells. Nature 448, 313-317 (2007)、 Wernig, M.等 人 In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature 448, 318-324 (2007) , Yamanaka, S. Strategies and new developments in the generation of patient-specific pluripotent stem cells. Cell Stem Cell 1, 39-49 (2007)等等。本领域技术人员已知多种可以用于这样 的干细胞多能性因子。 优选地, 所述的多能性因子包括 Oct4、 Sox2 (任选 地, Soxl ) 、 C-myc (任选地 L-Myc或 N-Myc ), 以及 Klf4 (任选地, Klf5或 Klf2 )、 Esrrb、 Nanog. 以及 Lin28。 上述多能性因子可以根据所 要导入的细胞而是任何来源的, 优选为小鼠的多能性因子以及其变体, 如 Sox2, NCBI登录号为 NM 011443.3 (小鼠包含 SRY-框的基因 2 ); Oct4, NCBI登录号为 NM— 013633.2 (小鼠 POU 结构域, 5 类, 转录因子 1 (Pou5fl) ) ; Klf4, NCBI登录号为 ΝΜ_010637·2 (小鼠 Kruppel-样因子 4) ) ; c-Myc , NCBI登录号为 NM— 010849.4 (小鼠髓细胞瘤病癌基因 ( myelocytomatosis oncogene ) (c-Myc) ) ; Soxl, NCBI 登录号为 NM— 009233.3, (小鼠包含基因 1 的 SRY-框); Klf2, NCBI登录号为 NM 008452.2, (小鼠 Kruppel-样因子 2(肺) ); Klf5, NCBI登录号为 匪 009769.4, (小鼠 Kruppel-样因子 5 ) ; Nanog , NCBI登录号为 NM 028016;或者 Lin28, NCBI登录号为 NM 145833。 C-Myc还可以被换 为其突变体 L-myc (登录号为 NM 008506.2,小鼠 v-myc髓细胞瘤病病毒 爱基因同源物 lv, 肺癌来源的 (禽类) ( (Mycll) ) );或者 N-Myc (登录号 为 NM— 008709.3, 小鼠 v-myc髄细胞瘤病病毒相关癌基因,神经母细胞瘤 衍生的 (禽类)(Mycn)。
本文所述的术语 "诱导重编程" (有时也仅被筒化为 "诱导" )是指 将体细胞去分化为多能性干细胞的过程。 优选地, 通过将维持干细胞多能 性所需的多能性因子 cDNA导入体细胞可以诱导体细胞去分化成为多能性 干细胞 ( Takahashi K, Yamanaka S. CelL 2006;126:663-676; Wernig M, Meissner A, Foreman R,等人, ature. 2007;448:318-324; Yu J, Vodyanik MA, Smuga-Otto K等人 Science. 2007;318:1917-1920 )。 其中, 优选地, 所述的多能性因子包括 Oct4、 Sox2 (任选地, Soxl ) 、 C-myc (任选地 L-Myc或 N-Myc ) 、 Klf4 (任选地, Klf5或 Klf2 ) 、 Nanog, 以及 Lin28 中的一个或多个。
将所述干细胞多能性因子 cDNA导入体细胞的方法可以是本领域技术 人员熟知的多种技术, 包括病毒感染、 脂质体转染、 转座子介导的***表 达、 穿膜蛋白、 药物诱导、 电穿孔、 粒子轰击等各种将 DNA转入细胞的 方法。 优选地, 使用包含 cDNA的病毒载体进行转染, 所述病毒载体包括 慢病毒载体、逆转录病毒栽体等多种病毒载体。优选地逆转录病毒载体(例 如 pMX载体) , 如实施例中所述的。
本文所述的 "适宜细胞生长的条件"是本领域的常规干细胞培养条件, 并且包括一些适宜各个具体细胞系, 但是不影响细胞基本性质的修饰, 培 养方法以及培养 ^参见 W. French Anderson等人, HANDBOOK OF STEM CELLS, 卷 2。
本文所述的报告基因是指能够指示细胞通过外加诱导已经转变为类似 胚胎干细胞的阶段, 包括利用转基因或同源重组手段加入一段表达荧光蛋 白或者抗性的序列, 这段序列处在胚胎干细胞特异表达的一些基因的启动 子的控制下, 故而可以在细胞到达胚胎干细胞状态时激活这段荧光蛋白或 抗性基因的表达, 从而使这个细胞具有某些可以被检测的性状特征(如发 出绿光)而区别于其他未重编程至该状态的细胞。 本领域技术常用的报告 基因包括绿色荧光蛋白, 抗性基因例如氨苄青霉素抗性基因等。 本领域的 技术人员可以根据细胞的培养条件和用途选择适合于各种实施方案的报告 基因。参考例如 Young II Yeom等人, Germline regulatory element of Oct-4 specific for the totipotent cycle of embryonal cells, Development 122,000-000(1996), Printed in Great Britain, The company of Biologists Limited 1996, 881-894 Shin-ya Hatano等人, Pluripotential competence of cells associated with Nanog activation, Mechanisms of Development 122(2005), 67-79。 ―
本文所述的检测细胞多能性的方法是本领域技术人员熟知的, 参见例 如 Yamanaka, S. Strategies and new developments in the generation of patient-specific pluripotent stem cells. Cell Stem Cell 1, 39-49 (2007)等。 所 述方法包括鉴定多能性分子标记的表达、 细胞的甲基化状态检测、 胚胎小 体 EB的形成、 畸胎瘤的形成以及施用经诱导的多能性干细胞形成嵌合鼠 等等。
本文所述的 "嵌合鼠" 是通 领域普通技术人员熟知的 "嵌合鼠" 技术实施的。 是指将胚胎干细胞或通过本文技术获得的 iPS细胞注射入小 鼠嚢胚中, 使得其与所注射入小鼠的嚢胚中的胚胎细胞混合, 共同在*** 母鼠中的子宫内生长发育, 小鼠出生后全身上下的各个组织即由两种胚胎 细胞共同混合组成,如马赛克拼图样,这样的小鼠被称为嵌合鼠(Evans M J,等人; The ability of EK cell to form chimeras after selection of clones in G418 and some observation on the intergration of retroviral vector proviral DNA into EK cells [M];Cold Spring Harbor Symposia on Quantitative Biology; 1985年; Xian MW, Wu BY, Hu XL, Shang KG, Wu HL, 1996. Construction of chimeric mice of ES cells by microinjection method. Hereditas (北京) 18 (1): 7-10 (中文))。使用 iPS能否形成嵌合鼠是 检验 iPS是否与胚胎干细胞具有类似性质的最直接和最关键的证据。
本文所述的 "筛选化合物" 是指在一个指定的化合物库中, 通过对报 告基因或者细胞本身特性的检测, 来筛选 1.对 iPS过程和诱导 ^有影响 的化合物; 2.能够取代目前已知的诱导 iPS过程所需的某个至全部因子的 化合物。 本领域技术人员已经开发了利用 iPS过程来筛选化合物的方法, 参见例如 Yan Shi等人, Induction of Pluripotent Stem Cells from Mouse Embryonic Fibroblasts by Oct4 and Klf4 with Small-Molecule Compounds. Cell Stem Cell 3, 568-574, 2008年 11月 6日。
本文所述的 "高通量" 筛选方法是本领域技术人员已知的, 是指利用 自动化的仪器设备和较小的样品使用量, 在较短的时间周期内可以实施对 一个大量文库中每个个体在特定的实验模型中所起作用的筛选。参见例如,
Nil Emre, 等人, A chemical approach to stem cell biology, Current Opinion in Chemical Biology 2007, 11:252-258。 在本方法中, 是用于在大 数量级化合物库、 天然产物库中针对 iPS过程进行高通量筛选。 实施例
下列实施例举例了发明人的标准实验室实践, 用于示例本发明的模式, 而 不应将本发明理解为限定于这些实施例的范围。 这些实施例。 根据本文公开和 本领域技术人员的普遍水平, 技术人员将理解下列仅用于示例, 可以在不超过 本发明的范围内进行各种变动、 修饰和改造。 其中所涉及的技术, 除非特别说 明, 均是本领域技术人员熟知的分子生物学、 细胞生物学、 生物化学等各个领 域的常规技术。
本发明中所用技^ L述:
除了特别说明, 本文中提及的各种物质均来自英杰生命科学公司
( Invitrogen )
细胞培养:
小鼠胚胎成纤维细胞来自 Oct-GFP转基因等位基因和 Rosa26等位基因的 半合子的 el3.5的胚, 并且培养在下述成纤维细胞培养基中: 高葡萄糖 DMEM, 补充有 10%FBS, L-谷氨酰胺和 NEAA。 iPS和 ES细胞均在包括血清的培养基 ( mES )和无血清的培养基 ( mKSR )中的 MEF饲养层上培养。 各种培养基在 本文中的名称以及组分洋见表 1。 MEF饲养层细胞由丝裂霉素 C灭活。
表 1: 所用的培养基
组成 培养 mES mKSR FB-SF1 iPS-SFl
基名
基础培养 高葡萄糖 Knock Out DMEM/F12(1:1) 高 葡萄糖 基 DMEM DMEM DMEM
血清或代 15%FBS 10%KOSR 10%KOSR 10%KOSR 血清添加 0.5% N2 0.5% N2
生长因子 1000U/mL lOOOU/mL lOOOU/mL LIF lOOOU/mL LIF LIF 5ng/mL bFGF LIF
( Millipore 5ng/mL 公司) bFGF
其它组分 2mM L-谷 2mM L-谷 2mM L-谷氨酰 2mM L-谷
氨酰胺 氨酰胺 胺 氨酰胺
1/100 1/100 1/100 NEAA 1/100
NEAA NEAA MEM NEAA
MEM MEM 青霉素 /链霉素 MEM
O.lmM O.lmM O.lmM
2-巯基乙醇 2-巯基乙醇 2-絲乙醇
ImM 丙酮 青霉素 /链 青霉素 /链 義 霉素 霉素
青霉素 /链
霉 素
( Hyclone
公司)
反转录病毒生产以及产生 iPS细胞
从 Addgene公司购置包含小鼠 Oct4、 Sox2、 Klf4和 c-Myc的 DNA的反转 录病毒载体(pMXs )。 按照现有技术进行病毒的产生和感染(Qin, D., Li, W., Zhang, J. & Pei, D. Direct generation of ES-like cells from unmodified mouse embryonic fibroblasts by Oct4/Sox2/Myc/Klf4. Cell research 17, 959-962 (2007); Qin, D.等人 Mouse meningiocytes express Sox2 and yield high efficiency of chimeras after nuclear reprogramming with exogenous factors. J Biol Chem 283, 33730-33735 (2008). )简言之, 利用常规方法将这些质粒转染到 PlatE细胞中。 然后收集病毒上清液并且过滤 48小时,以感染 MEF,其中补充有 1,5-二曱基 -1,5- 二氮十一亚曱基聚曱溴化物。 在第二天重复相同的步骤。 移除病毒上清液的当 天被定义为感染后 0天。 将病毒感染后(即已经转染了 Oct4、 Sox2、 Klf4和 / 或 c-Myc, 下文中, 如无特殊说明, 3因子感染代表用 Oct4、 Sox2、 Klf4感染, 4因子感染代表用 Oct4、 Sox2、 Klf4和 c-Myc感染)的成纤维细胞培养在本发 明的培养基中, 在感染后 10-15天挑取 iPS集落, 这 于 Oct-GFP (即在荧 光显微镜下发射荧光的集落)和典型的 ES形态来 ^^的。 随后如 ES细胞样延 展和保持^^的集落(如上所述(^11,0.,1^,\¥.,21131^, & ?61,0, 2007; 以及 Qin, D.等人, 2008 )。
重编程效率的定量
用于定量重编程效率的主要方法是 Oct4-GFP阳性细胞的 FACS分析。 基 于计时过程的结果,在感染后第 7天和第 9天用胰蛋白酶消化经感染的 MEF细 胞, 然后通过 FACSCalibur分析。 GFP阳性的细胞收到来自 PE通道的控制信 号的门控,最少记录 15000个事件。用 pMXs-FLAG感染的细胞作为阴性对照。 为了证实由 FACS分析的效率, 直接在荧光显微镜下对感染后第 14天的 GFP- 阳性集落计数。
iPS细胞的表征:
进行碱性磷酸酶和免疫荧光染色(如上所述 Qin, D., Li, W., Zhang, J. & Pei, D, 2007; 以及 Qin,D.等人, 2008 )。使用下列一抗: 小鼠抗 -Oct4 ( Santa Cruz 公司)、 小鼠抗 -SSEA1 (Abeam公司), 小鼠抗 -Nanog (Abeam公司)。 实施例 1: 传统的 mKSR培养基不能维持用 4个因子转染的 MEF的生长, 同时也不能诱导形成 iPS
如前所述, 将四因子的病毒以 1:1:1:1混合(每种 lml )后感染到六孔板的 一孔中共计 3.5万个成纤维细胞中, 并且在 37度, 5 % C02分别培养在 mKSR 和 mES培养基中。
如图 1所示, 在 mKSR中培养的 4因子转染的成纤维细胞生长迟緩, 在 12 天时依然没有形成多能性细胞的细胞集落形态, 即 mKSR培养基不能够诱导和 产生 iPS集落。 实施例 2: 应用 iPS-SFl培养基能够极大地提高诱导 iPS的效率
A.分别将 MEF培养在 FBS和 iPS-SFl培养基中, 其生长曲线几乎一致, 表明 iPS-SFl对于 MEF的培养无影响,相对的,在 mKSR中 MEF的生长基本 停止。 (图 2A )
在分别用 3个因子(Oct4、 Klf4和 Sox2 )或者 4个因子(cMyc、 Oct4、 Klf4和 Sox2 )转染到成纤维细胞后, 将所述经转染的细胞分别培养在 iPS-SFl 培养基中, 在转染后第 2、 5和 7天如前所述测定 GFP阳性细胞的效率, 观察 到无论是用 4个因子还是用 3个因子转染的细胞, 均表现出高效率(图 2B ), 在第 7天甚至均达到 18%,相对于传统方法(图示 mES对照)有非常大的提高。
相应的,在这一过程中,采用荧光显微镜直接观察,也可以发现采用 iPS-SFl 培养基培养的经转染细胞,在转染后第 6天即有强表达 Oct4-GFP的类胚胎干细 胞克隆出现, 而在传统培养基中形成的克隆则没有荧光表达(图 2C )。
同样,如图 9所示,在第 7天分别收获用 3因子和 4因子感染的 MEF细胞 后, 对其进行 FACS效率分析, 表明在 iPS-SFl中, 经感染的 MEF细胞的 iPS 重编程效率 (如上文所述, 即用 Oct4-GFP阳性细胞占总细胞的百分比表示)远大 于在常规的 mES培养基中 (iPS-SFl vs mES: 3因子: 0.07% vs 17.82%; 4因 子: 0.10 % vsl5.07% )。 实施例 3: iPS-SFl诱导的 iPS具有多能性。
A经诱导得到的 iPS细胞系具有与胚胎干细胞相似的细胞形态
如上所述, 用 3因子感染成纤维细胞, 并之后将其一直在 iPS-SFl培养基 中培养, 在感染后第 12 - 14天, 根据克隆形态及荧光表达 有代表意义的克 隆团, 经过数代的稳定传代后形成均匀的 iPS细胞系。
如图 2A所示, 左图是正常的胚胎干细胞, 右图: 上述 iPS细胞系。 这些细 胞系形态与胚胎干细胞非常相似, 强烈表达 Oct4-GFP的绿色荧光。
B嵌合鼠的形成 经诱导得到的 iPS细胞系具有多能性
构建嵌合鼠
注射用嚢胚取自四周龄超排 ICR母小鼠(白色), 与同品系公鼠合笼, 见栓 3.5 d (当日见栓为 0.5 d) 时从子宫和输卵管中收集胚胎, 移入上覆石 蜡油的 M 16培养液滴内, 于 37 C5 % C02 孵箱内培养。
注射前 3h将嵌合用 iPS细胞(即上文中所得到的 iPS细胞系)更换 新鲜培养液,胰酶消化后制成单细胞悬液备用。 将适量 iPS 细胞悬液和嚢 胚期胚胎移入 M2 注射液滴内,在显微注射***下, 用持卵针固定嚢胚, 用注射针吸取 IPS 细胞,从远离内细胞团的滋养层部位进针,每个嚢胚注 射细胞 10 - 12 个。注射后嚢胚腔消失, 置于培养液中于孵箱中培养 1 - 3 h , 待嚢胚腔恢复后,移入见栓 2.5 d 的假孕母鼠子宫中进行培育。
图 3-B显示了使用本发明方法获得的 iPS构建的嵌合鼠, 其中非嵌合 小鼠为纯白色, 嵌合小鼠由于注射的 iPS细胞来源于黑色的 OG2/Rosa26 小鼠, 故呈现黑色与白色相间分布的体色, 提示经本发明方法获得的 iPS 细胞可以参与正常的体内发育并形成嵌合体小鼠, 提示这些细胞在发育上与胚 胎干细胞没有差异。 实施例 4: 本发明培养基对于 iPS诱导过程中多能性基因启动子去曱基化的 加速
将培养在 mES培养基和本发明 iPS-SFl培养基中的经 Oct4、 Sox2、 Klf4三 因子感染(如上所述制备)的细胞系中获取基因组 DNA(700ng), 通过将其暴露 于 50.6%的亚疏酸氢钠 ( Sigma S-1516 )和 10mM氢醌( Sigma H-9003 )的混 合物过夜来进行亚硫酸氢盐修饰。通过 PCR使用表 2中所述的引物组对 Nanog 的启动子区域进行扩增。 将 PCR产物克隆到平 pMD8-T载体( Takara公司) 中, 在 DH5a中增殖, 并且测序。
表 2
Figure imgf000021_0001
在 iPS诱导过程中,最终需要激活内源表达的多能性基因从而自发地维持 iPS 自我更新而不依赖于外源表达, 因此伴随重编程, 多能性基因的启动子存在去 曱基化的过程, 本例用 Oct4、 Sox2、 Klf4三种病毒 1:1:1感染 MEF, 并分别培 养在传统的 mES培养基中及本发明培养基 iPS-SFl中, 在感染后 2天和 6天收 取细胞样本, 提取基因组 DNA, 将 DNA酶切后用重硫酸盐处理过夜, 使得未 甲基化的 CpG ^基对中的 C (胞嘧啶)变化为 U (尿嘧啶), 之后用巢式 PCR 扩增出 Nanog的启动子, 交由本领域已知的测序公司测序(例如 Invitrogen公 司), »测序结果分析 iPS过程中 Nanog启动子的曱基化变化状态。
如图 7所示, 3因子感染后的 MEF, 在 mES中緩慢地进行着 Nanog启动子 的去曱基化(D2: 33%, D6: 41.7 % ), 而在本发明培养基( iPS-SFl ) 中则进 行得极为迅速, 在 D2就已经达到 66.7 %的去曱基化状态, 并且持续进行, 在 D6时去甲基化水平已经达到 80.9 % ,表示 Nanog启动子中被检测的该片段已经 基本被激活,而 Nanog这一典型的干细胞多能性因子的激活标志着 iPS被诱导。 通过上述实施例可以看出, 本发明的 iPS-SFl培养基不仅能够成功诱 导 iPS的重编程, 并且该诱导重编程可以避免使用有致癌作用的 c-Myc基 因进行感染, 而且在重编程的效率、 去曱基化的速度都远高于现有的 mES 培养基。 实施例 5: 本发明的培养基中各组分对于 iPS重编程效率的影响
将表 1中所列出的 iPS-SFl培养基中的 LIF、 N2和 bFGF分别缺失, 或者 在 DMEM+10 % KOSR (即不含生长因子, 同时含有 NEAA, L-Glutamine等成 份, 下文称为 bKSR )培养基中分别以与 iPS-SFl相当的浓度分别再加入 N2和 bFGF, 测 LIF、 N2、 bFGF对于用 4因子转染的 MEF细胞的 iPS重编程 效率的影响。 图 4中各个标记的含义如下:
Mock: bKSR
Mock+LIF: bKSR+LIF
Mock+N2: bKSR-LIF +N2
Mock+bFGF: bKSR-LIF +bFGF
iPS-SFl— LIF:无 LIF的 iPS-SFl
iPS-SFl -N2: 无 N2的 iPS-SFl
iPS-SFl -bFGF: 无 bFGF的 iPS-SFl
由此可见, bFGF对于 iPS重编程效率的影响甚至大于本领域已知的 LIF。 实施例 6: 血清对于用 iPS-SFl培养基培养的 iPS重编程的抑制作用 如图 5所示, 当将 iPS-SFl培养基中分别加入 2%、 4%、 6%、 8%和 10% 的血清时, 与已知的现有技术所不同的, 血清将 iPS重编程的效率抑制了最高 达 96%。 实施例 7: 其它酪氨酸激酶对于本发明培养基的影响 导 iPS重编程过程起作用, 将 4因子的病毒感染 MEF后, 换成以下培养基, 其 中空白对照是不含 bFGF的 iPS-SFl,实验组为在空白培养基(即不含 bFGF的 iPS-SFl ) 中添加了各种受体酪氨酸激酶(包括碱性成纤维生长因子 bFGF, 表 皮生长因子 EGF,血管内皮生长因子 VEGF,***二 IGF2, 以及 雄二醇, 血清培养基(FBS )作为该实验的阴性对照。 如图 8所示, 除了 bFGF外, 、 IGF2、 VEGF以及 ***均对于 iPS重编程过程的诱导有相当的作用 实施例 8: 在 iPS-SFl培养基中的 iPS过程中筛选化合物
将 20, 000个 MEF细胞种植在 12孔平板的每孔中, 培养基为 iPS-SFl, 然后如上所述进行病毒感染, 以转染 4个因子。 将如图 6所示的各种化合物以 下述浓度分别添加到培养基中: ΡΟ0325901(1μΜ)、 CHIR99021(3 M)、 δυ5402(2μΜ, EMDbiosciences)、 Υ-27632(10μΜ)、维生素 Ε(25μΜ, Sigma公司)、 维生素 Α(1μΜ, Sigma 公司)、 Α83-01(0.5μΜ, EMD)、 8Β203580(2μΜ) ,
Dorspmorphin(3 M, Sigma公司)、 ΡΡΓ-α(ΙΟμΜ), TSA(20nM, Sigma公司)、
VPA(lmM, EMD)、 JAK抑制剂(0.3μΜ, EMD)、 5aza-DC(l M, Sigma公司)、
ΒΙΧ01294(1μΜ), Bayk8644(2 M, EMD公司)。
在感染后第 7天如上所述收集细胞用于 FACS分析, 测定相对的 GFP效率。 可将 TSA和 VPA能够显著增强 iPS重编程的效率( 100% )。而在使用本发明的 培养基的条件下, 原来报道有利于小鼠胚胎干细胞自我更新的化合物例如
PD0325901. CHIR99021. SU5402降低了 iPS重编程的效率。

Claims

权 利 要 求 书
1.诱导多能性干细胞的无血清培养基,其包含基础培养基、代血清添
2.权利要求 1所述的无血清培养基,其还包含白血病抑制因子 ( LIF )。
3.权利要求 1 所述的培养基, 其中基础培养基包括但不限于 Dulbecco's Modified Eagle's Medium (DMEM), Minimal Essential Medium (MEM), Basal Medium Eagle (BME), F-10, F-12, RPMI 1640, Glasgow's Minimal Essential Medium (GMEM), aMinimal Essential Medium (aMEM), Iscove's Modified Dulbecco's Medium和 M199。
4.权利要求 1所述的培养基,其中代血清添加剂包含支持细胞生长的 至少一种或多种以下组分: 一个或多个胰岛素及胰岛素替代物、一个或多 个转金属蛋白及转金属蛋白替代物、一个或多个微量元素、一个或多个维 生素、一个或多个氨基酸、一个或多个激素及类激素化合物、血清白蛋白 或血清白蛋白替代物、 以及一个或多个脂类。
5.权利要求 1-4 所述的培养基, 其中代血清添加剂选自 KonckOut Serum Replacement ( KOSR ) , N2, B27, Insulin-Transferrin-Selenium Supplement (ITS)和 /或 G5。
6.权利要求 5 所述的培养基, 其中代血清添加剂是由 KnockOut Serum Replacement ( KOSR )、 N2和 /或 B27所调配而成的
7.权利要求 6所述的培养基, 其中在最终培养基中 KOSR的浓度在 5%-20%之间。
8.权利要求 6或 7所述的培养基, 其中在最终培养基中 的浓度在 0%-1%之间。
9.权利要求 6-8 中任一项所述的培养基, 其中在最终培养基中 B27 的浓度在 0%-2%之间。
10. 权利要求 6所述的培养基,其中在最终培养基中 KOSR的浓度 在 5%-20%之间、 的浓度在 0%-1%之间, 以及 B27的浓度在 0%-2% 之间。
11. 权利要求 5所述的培养基, 其中代血清添加剂是由 KOSR和 N2所调配而成的。
12. 权利要求 11所述的培养基, 其中在最终培养基中 KOSR的浓 度为 5%-20%, N2的浓度为 0%-1%。
13. 权利要求 12所述的培养基, 其中在最终培养基中 KOSR的浓 度为大约 10%, N2的浓度为大约 0.5%。
14. 权利要求 1至 13中任一项所述的培养基,其中酪氨酸激酶选自 bFGF、 EGF、 IGF2或 VEGF中的至少一种。
15. 权利要求 14所述的培养基, 其中酪氨酸激酶为 bFGF。
16. 权利要求 15所述的培养基,其中在最终培养基中 bFGF的浓度 在 3-20ng/mL之间。
17. 权利要求 15所述的培养基,其中在最终培养基中 bFGF的浓度 在 5-15ng/mL之间.
18. 权利要求 17所述的培养基,其中在最终培养基中 bFGF的浓度 为大约 5ng/mL或大约 7ng/mL。
19. 权利要求 14所述的培养基, 其中酪氨酸激酶为 EGF。
20. 权利要求 19所述的培养基, 其中在最终培养基中 EGF的浓度 为大约 10ng/mL。
21. 权利要求 14所述的培养基, 其中酪氨酸激酶为 IGF2。
22. 权利要求 21所述的培养基, 其中在最终培养基中 IGF2的浓度 为大约 25ng/mL。
23. 权利要求 14所述的培养基, 其中酪氨酸激酶为 VEGF。
24. 权利要求 23所述的培养基, 其中在最终培养基中 VEGF的浓 度为大约 10ng/mL。
25. 权利要求 1至 24中任一项所述的培养基,其中所述培养 ^£包 含适于细胞生长的其它组分。
26. 权利要求 25所述的培养基, 其中其它组 ^^自 L-谷氨酰胺、 NEAA MEM, 丙酮酸钠和 2-巯基乙醇。
27. 从体细胞高效率诱导多能性干细胞的方法, 其包括
( a ) 将一个或多个干细胞多能性因子导入体细胞;
( ) 用权利要求 1-26中任一项所述的培养基,在适合于细 胞生长的条件下, 培养(a ) 中经导入的体细胞, 以诱导体细胞成 为多能性干细胞;
( c ) 检测并且分析经诱导的细胞的多能性;
( d ) 挑出具有多能性的经诱导的多能性干细胞的单克隆;
( e ) 在适于胚胎干细胞生长的条件下在胚胎干细胞培养 基中培养 ) 中的单克隆细胞。
28. 权利要求 27所述的方法,其中转录因子选自 Oct4、 Sox2、 Klf4、 c-Myc、 Nanog、 Esrrb和 Lin28。
29. 权利要求 25的方法, 其中导入的方法选自病毒感染、转染、转 座子介导的***表达、 穿膜蛋白或药物诱导。
30. 权利要求 29所述的方法,其中病毒感染为使用逆转录病毒或慢 病毒。
31. 权利要求 27的方法, 其中所述体细胞来源于哺乳动物。
32. 权利要求 31的方法, 其中所述体细胞来源于人、 猴、 猪、 狗、 猫、 大鼠或小鼠。
33. 权利要求 32的方法, 其中所述体细胞来源于小鼠。
34. 权利要求 27-33中任一项的方法, 其中所述体细胞为成纤维细 胞或者脑膜细胞。
35. 权利要求 27-34中任一项的方法, 其中检测细胞多能性的方法 包括鉴定多能性分子标记的表达、 细胞的 DNA甲基化状态检测、 胚胎小 体 EB的形成、畸胎瘤的形成或使用经诱导的多能性干细胞的嵌合鼠的形 成。
36. 权利要求 1-26中任一项所述的培养基用于高效率诱导体细胞生 成多能性干细胞的用途。
37. 权利要求 36的用途, 其中所述体细胞来源于哺乳动物。
38. 权利要求 37的用途, 其中所述体细胞来源于人、 猴、 狗、 猫、 大鼠或小鼠。
39. 权利要求 38的用途, 其中所述体细胞来源于小鼠。
40. 权利要求 36-39中任一项的用途, 其中所述体细胞为成纤维细 胞或者脑膜细胞。
41. 筛选化合物的方法, 包括:
( a ) 将一个或多个干细胞多能性因子导入体细胞;
( ) 用权利要求 1-26中任一项所述的培养基,在适合于细 胞生长的条件下, 培养(a ) 中经导入的体细胞, 以诱导体细胞成 为多能性干细胞;
( c ) 检测并且分析经诱导的细胞的多能性;
( d ) 挑出具有多能性的经诱导的多能性干细胞的单克隆;
( e ) 在适于胚胎干细胞生长的条件下在胚胎干细胞培养 基中培养 ) 中的单克隆细胞。
( f ) 应用 (d ) 中所培养的经诱导的多能性干细胞筛选化 合物。
42. 权利要求 41的方法, 其中 (f ) 中的筛选化合物为高通量筛选 化合物。
43. 权利要求 41或 42的方法, 其中所述体细胞来源于哺乳动物。
44. 权利要求 43的方法, 其中所述体细胞来源于人、 猴、 狗、 猫、 大鼠或小鼠。
45. 权利要求 44的方法, 其中所述体细胞来源于小鼠。
46. 权利要求 41-45中任一项的方法, 其中所述体细胞为成纤维细 胞或者脑膜细胞。
47. 权利要求 41-46中任一项的方法,其中检测细胞多能性的方法包括 鉴定多能性分子标记的表达、 细胞的甲基化状态检测、胚胎小体 EB的形 成、 畸胎瘤的形成或使用经诱导的多能性干细胞的嵌合鼠的形成。
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