WO2005019460A3 - Promotoren zur expression von genen in tagetes - Google Patents

Promotoren zur expression von genen in tagetes Download PDF

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Publication number
WO2005019460A3
WO2005019460A3 PCT/EP2004/008624 EP2004008624W WO2005019460A3 WO 2005019460 A3 WO2005019460 A3 WO 2005019460A3 EP 2004008624 W EP2004008624 W EP 2004008624W WO 2005019460 A3 WO2005019460 A3 WO 2005019460A3
Authority
WO
WIPO (PCT)
Prior art keywords
expression
tagetes
promoter
genes
promoters
Prior art date
Application number
PCT/EP2004/008624
Other languages
English (en)
French (fr)
Other versions
WO2005019460A2 (de
Inventor
Matt Sauer
Christel Renate Schopfer
Ralf Flachmann
Karin Herbers
Irene Kunze
Martin Klebsattel
Thomas Luck
Dirk Voeste
Angelika-Maria Pfeiffer
Original Assignee
Sungene Gmbh & Co Kgaa
Matt Sauer
Christel Renate Schopfer
Ralf Flachmann
Karin Herbers
Irene Kunze
Martin Klebsattel
Thomas Luck
Dirk Voeste
Angelika-Maria Pfeiffer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP2003/009106 external-priority patent/WO2004018694A2/de
Priority claimed from PCT/EP2003/009101 external-priority patent/WO2004018688A1/de
Application filed by Sungene Gmbh & Co Kgaa, Matt Sauer, Christel Renate Schopfer, Ralf Flachmann, Karin Herbers, Irene Kunze, Martin Klebsattel, Thomas Luck, Dirk Voeste, Angelika-Maria Pfeiffer filed Critical Sungene Gmbh & Co Kgaa
Priority to EP04763695A priority Critical patent/EP1658371A2/de
Priority to US10/568,741 priority patent/US20060162020A1/en
Publication of WO2005019460A2 publication Critical patent/WO2005019460A2/de
Publication of WO2005019460A3 publication Critical patent/WO2005019460A3/de
Priority to IL173780A priority patent/IL173780A0/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Die vorliegende Erfindung betrifft die Verwendung von Promotoren ausgewählt aus der Gruppe A) EPSPS Promotor, B) B-Gene Promotor, C) PDS Promotor und D) CHRC Promotor zur Expression, vorzugsweise zur blütenspezifischen Expression von Genen in Pflanzen der Gattung Tagetes, die genetisch veränderten Pflanzen der Gattung Tagetes sowie ein Verfahren zur Herstellung von biosynthetischen Produkten durch Kultivierung der genetisch veränderten Pflanzen.
PCT/EP2004/008624 2003-08-18 2004-07-31 Promotoren zur expression von genen in tagetes WO2005019460A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP04763695A EP1658371A2 (de) 2003-08-18 2004-07-31 Promotoren zur expression von genen in tagetes
US10/568,741 US20060162020A1 (en) 2003-08-18 2004-07-31 Promoters for the expression of genes in tagetes
IL173780A IL173780A0 (en) 2003-08-18 2006-02-16 Promoters for the expression of genes in tagetes

Applications Claiming Priority (14)

Application Number Priority Date Filing Date Title
EPPCT/EP/03/09109 2003-08-18
EPPCT/EP/03/09107 2003-08-18
EPPCT/EP/03/09102 2003-08-18
PCT/EP2003/009106 WO2004018694A2 (de) 2002-08-20 2003-08-18 Verfahren zur herstellung von ketocarotinoiden in genetisch veränderten organismen
PCT/EP2003/009101 WO2004018688A1 (de) 2002-08-20 2003-08-18 VERFAHREN ZUR HERSTELLUNG VON β-CAROTINOIDEN
EPPCT/EP/03/09105 2003-08-18
PCT/EP2003/009109 WO2004017749A2 (de) 2002-08-20 2003-08-18 Verwendung von astaxanthinhaltigen pflanzen oder pflanzenteilen der gattung tagetes als futtermittel
PCT/EP2003/009102 WO2004018693A2 (de) 2002-08-20 2003-08-18 Verfahren zur herstellung von ketocarotinoiden in blütenblättern von pflanzen
PCT/EP2003/009105 WO2004018385A2 (de) 2002-08-20 2003-08-18 Verfahren zur herstellung von zeaxanthin und/oder dessen biosynthetischen zwischen- und/oder folgeprodukten
EPPCT/EP/03/09101 2003-08-18
PCT/EP2003/009107 WO2004018695A2 (de) 2002-08-20 2003-08-18 Verfahren zur herstellung von ketocarotinoiden in früchten von pflanzen
EPPCT/EP/03/09106 2003-08-18
DE102004007623.5 2004-02-17
DE102004007623A DE102004007623A1 (de) 2004-02-17 2004-02-17 Promotoren zur Expression von Genen in Tagetes

Publications (2)

Publication Number Publication Date
WO2005019460A2 WO2005019460A2 (de) 2005-03-03
WO2005019460A3 true WO2005019460A3 (de) 2005-07-21

Family

ID=34801928

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/008624 WO2005019460A2 (de) 2003-08-18 2004-07-31 Promotoren zur expression von genen in tagetes

Country Status (4)

Country Link
US (1) US20060162020A1 (de)
DE (1) DE102004007623A1 (de)
IL (1) IL173780A0 (de)
WO (1) WO2005019460A2 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA05001944A (es) * 2002-08-20 2005-06-22 Sungene Gmbh & Co Kgaa Procedimiento para elaborar cetocarotenoides en frutos de plantas.
EP2133424A1 (de) * 2004-11-29 2009-12-16 Kirin Beer Kabushiki Kaisha Peptidtransport zu den Chromoplasten in Blütenblättern und Verfahren zur Bildung einer Pflanze mit gelblichen Blütenblättern damit
DE102005021365A1 (de) * 2005-05-04 2006-11-09 Sungene Gmbh & Co. Kgaa Transgene Expressionskassetten zur Expression von Nukleinsäuren in Blütengeweben von Pflanzen
WO2007032568A1 (en) * 2005-09-15 2007-03-22 Korea Advanced Institute Of Science And Technology Method for improving organisms using flux scanning based on enforced objective flux
EP2032705A2 (de) * 2006-06-13 2009-03-11 BASF Plant Science GmbH Verwendung eines plastid-lipid associated protein-promotors (pap-promotors) zur heterologen genexpression
US20100021962A1 (en) * 2006-06-13 2010-01-28 Basf Plant Science Gmbh Use of A Plastid-Lipid Associated Protein Promoter (PAP Promoter) For Heterologous Gene Expression
WO2008058948A1 (en) * 2006-11-14 2008-05-22 Basf Plant Science Gmbh Plastid-lipid-associated protein promoters for the production of keto-carotenoids in tagetes
WO2008058946A1 (en) * 2006-11-15 2008-05-22 Basf Plant Science Gmbh New ketolases for the production of ketocarotenoids in tagetes
US8222388B2 (en) * 2006-11-22 2012-07-17 Ceres, Inc. Broadly expressing regulatory regions
US9150877B2 (en) * 2007-09-27 2015-10-06 Dow Agrosciences Llc Construct and method for expressing transgenes using a Brassica bidirectional constitutive promoter
EP2199399A1 (de) 2008-12-17 2010-06-23 BASF Plant Science GmbH Ketokarotinoidproduktion in Pflanzen
WO2010079032A1 (en) * 2008-12-17 2010-07-15 Basf Plant Science Gmbh Production of ketocarotenoids in plants
CA2786697C (en) 2010-01-14 2018-10-30 Stanislaw Flasinski Plant regulatory elements and uses thereof
EP3630943A4 (de) 2017-06-01 2021-06-09 Knipbio, Inc. Heterologe karotinoidproduktion in mikroorganismen
WO2024018037A1 (en) * 2022-07-20 2024-01-25 Bioinnova S.R.L.S. Microalgae expressing glp-2 and uses thereof
WO2024018036A1 (en) * 2022-07-20 2024-01-25 Bioinnova S.R.L.S. Microalgae expressing biologically active products

Citations (14)

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WO1998014465A1 (en) * 1996-10-01 1998-04-09 Colorado State University, Through Its Agent Colorado State University Research Foundation 1-aminocyclopropane-1-carboxylate synthase genes from pelargonium and rosa to control ethylene levels in geraniums and roses
WO1998024300A1 (en) * 1996-12-05 1998-06-11 Yissum Research Development Company Of The Hebrew University Of Jerusalem Carotenoid-associated proteins useful for high carotenoid accumulation and production in plants and other organisms
WO1999061399A1 (en) * 1998-05-26 1999-12-02 The University Of Maryland Genes encoding epsilon lycopene cyclase and method for producing bicyclic epsilon carotene
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DE10238980A1 (de) * 2002-08-20 2004-03-04 Sungene Gmbh & Co. Kgaa Verfahren zur Herstellung von Ketocarotinoiden in Blütenblättern von Pflanzen
DE10238978A1 (de) * 2002-08-20 2004-03-04 Sungene Gmbh & Co. Kgaa Verfahren zur Herstellung von Ketocarotinoiden in Früchten von Pflanzen
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EP0524910A2 (de) * 1991-07-25 1993-01-27 Sandoz Ltd. Modulation der Blütenfarbe
WO1998014465A1 (en) * 1996-10-01 1998-04-09 Colorado State University, Through Its Agent Colorado State University Research Foundation 1-aminocyclopropane-1-carboxylate synthase genes from pelargonium and rosa to control ethylene levels in geraniums and roses
WO1998024300A1 (en) * 1996-12-05 1998-06-11 Yissum Research Development Company Of The Hebrew University Of Jerusalem Carotenoid-associated proteins useful for high carotenoid accumulation and production in plants and other organisms
WO1999061399A1 (en) * 1998-05-26 1999-12-02 The University Of Maryland Genes encoding epsilon lycopene cyclase and method for producing bicyclic epsilon carotene
WO2000008920A1 (en) * 1998-08-14 2000-02-24 Yissum Research And Development Company Of The Hebrew University Of Jerusalem Polynucleotides controlling the expression of and coding for gene b in tomato
WO2000032788A2 (en) * 1998-11-30 2000-06-08 Chr. Hansen A/S Method for regulating carotenoid biosynthesis in marigolds
WO2000066747A1 (en) * 1999-04-29 2000-11-09 Syngenta Limited Herbicide resistant plants
WO2002061050A2 (en) * 2001-01-12 2002-08-08 University Of Maryland, College Park Methods for determining ring number in carotenoids by lycopene epsilon-cyclases and uses thereof
EP1323825A2 (de) * 2001-11-09 2003-07-02 Enea Ente Per Le Nuove Tecnologie, L'energia E L'ambiente Rekombinante Pflanzen und DNA Konstrukte
DE10238979A1 (de) * 2002-08-20 2004-02-26 Sungene Gmbh & Co. Kgaa Verfahren zur Herstellung von Zeaxanthin und/oder dessen biosynthetischen Zwischen- und/oder Folgeprodukten
DE10238980A1 (de) * 2002-08-20 2004-03-04 Sungene Gmbh & Co. Kgaa Verfahren zur Herstellung von Ketocarotinoiden in Blütenblättern von Pflanzen
DE10238978A1 (de) * 2002-08-20 2004-03-04 Sungene Gmbh & Co. Kgaa Verfahren zur Herstellung von Ketocarotinoiden in Früchten von Pflanzen
WO2004027069A1 (de) * 2002-08-20 2004-04-01 Sungene Gmbh & Co. Kgaa Transgene expressionskassetten zur expression von nukleinsäuren in der pflanzlichen blüte
DE10253112A1 (de) * 2002-11-13 2004-06-03 Sungene Gmbh & Co. Kgaa Verfahren zur Herstellung von Ketocarotinoiden in genetisch veränderten Organismen

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Also Published As

Publication number Publication date
US20060162020A1 (en) 2006-07-20
WO2005019460A2 (de) 2005-03-03
DE102004007623A1 (de) 2005-08-25
IL173780A0 (en) 2006-07-05

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