WO2003092361A3 - Temporal seed promoters for expressing genes in plants - Google Patents

Temporal seed promoters for expressing genes in plants Download PDF

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Publication number
WO2003092361A3
WO2003092361A3 PCT/US2003/013847 US0313847W WO03092361A3 WO 2003092361 A3 WO2003092361 A3 WO 2003092361A3 US 0313847 W US0313847 W US 0313847W WO 03092361 A3 WO03092361 A3 WO 03092361A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
present
expressing genes
seed promoters
temporal
Prior art date
Application number
PCT/US2003/013847
Other languages
French (fr)
Other versions
WO2003092361A2 (en
Inventor
Qi Wang
Lisa M Weaver
Tim N Oulmassov
Jeffrey Ahrens
Patrice Dubois
Jeffery Q Shen
Original Assignee
Monsanto Technology Llc
Qi Wang
Lisa M Weaver
Tim N Oulmassov
Jeffrey Ahrens
Patrice Dubois
Jeffery Q Shen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc, Qi Wang, Lisa M Weaver, Tim N Oulmassov, Jeffrey Ahrens, Patrice Dubois, Jeffery Q Shen filed Critical Monsanto Technology Llc
Priority to CA2483782A priority Critical patent/CA2483782C/en
Priority to AU2003225283A priority patent/AU2003225283A1/en
Priority to CN038157748A priority patent/CN1728937B/en
Priority to EP03722003A priority patent/EP1549132A4/en
Priority to BR0309869-9A priority patent/BR0309869A/en
Publication of WO2003092361A2 publication Critical patent/WO2003092361A2/en
Publication of WO2003092361A3 publication Critical patent/WO2003092361A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to the field of plant genetic engineering. More specifically, the present invention relates to seed specific gene expression during a defined period of embryogenesis. The present invention provides promoters capable of transcribing heterologous nucleic acid sequences in seeds, and methods of modifying, producing, and using the same.
PCT/US2003/013847 2002-05-03 2003-05-05 Temporal seed promoters for expressing genes in plants WO2003092361A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA2483782A CA2483782C (en) 2002-05-03 2003-05-05 Temporal seed promoters for expressing genes in plants
AU2003225283A AU2003225283A1 (en) 2002-05-03 2003-05-05 Temporal seed promoters for expressing genes in plants
CN038157748A CN1728937B (en) 2002-05-03 2003-05-05 Temporal seed promoters for expressing genes in plants
EP03722003A EP1549132A4 (en) 2002-05-03 2003-05-05 Temporal seed promoters for expressing genes in plants
BR0309869-9A BR0309869A (en) 2002-05-03 2003-05-05 Seed time promoters for plant gene expression

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US37724702P 2002-05-03 2002-05-03
US60/377,247 2002-05-03
US10/429,555 US7179959B2 (en) 2002-05-03 2003-05-05 Temporal seed promoters for expressing genes in plants
US10/429,555 2003-05-05

Publications (2)

Publication Number Publication Date
WO2003092361A2 WO2003092361A2 (en) 2003-11-13
WO2003092361A3 true WO2003092361A3 (en) 2004-09-02

Family

ID=29406787

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/013847 WO2003092361A2 (en) 2002-05-03 2003-05-05 Temporal seed promoters for expressing genes in plants

Country Status (7)

Country Link
US (5) US7179959B2 (en)
EP (1) EP1549132A4 (en)
CN (1) CN1728937B (en)
AU (1) AU2003225283A1 (en)
BR (1) BR0309869A (en)
CA (1) CA2483782C (en)
WO (1) WO2003092361A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1728937B (en) * 2002-05-03 2011-03-30 孟山都技术公司 Temporal seed promoters for expressing genes in plants
US9238818B2 (en) * 2004-04-20 2016-01-19 Syngenta Participations Ag Methods and genetic constructs for modification of lignin composition of corn cobs
CN101914530B (en) * 2004-04-20 2014-06-04 辛根塔参与股份公司 Regulatory sequences for expressing gene products in plant reproductive tissue
MX2007004231A (en) 2004-10-08 2007-06-14 Dow Agrosciences Llc Certain plants with no saturate or reduced saturate levels of fatty acids in seeds, and oil derived from the seeds.
US20090031440A1 (en) 2005-02-26 2009-01-29 Basf Plant Science Gmbh Expression Cassettes for Seed-Preferential Expression in Plants
WO2007008546A2 (en) * 2005-07-08 2007-01-18 Renessen Llc High tryptophan soybean meal
CN101792761B (en) * 2009-10-23 2013-06-19 武汉大学 Induction-enhanced tissue specific promoter and application thereof
BR102012008162B1 (en) * 2012-04-09 2018-08-14 Empresa Brasileira De Pesquisa Agropecuária - Embrapa compositions and methods for modifying the expression of genes of interest
CN104480110B (en) * 2013-08-30 2017-03-29 中国农业科学院生物技术研究所 Corn tissue's specificity promoter and its application
CN104350911A (en) * 2014-10-22 2015-02-18 安徽科技学院 Method for interplanting leaf mustard for root with wheat
CN107099532B (en) * 2017-06-05 2019-12-13 中国农业科学院油料作物研究所 cabbage type rape embryo specific promoter pBnaA09g21960D and application thereof
CN108738668B (en) * 2018-06-26 2023-09-15 山东省农业科学院生物技术研究中心 Fresh dendrobium candidum strip harvester
CN109306352B (en) * 2018-11-09 2021-04-30 中国热带农业科学院三亚研究院 Papaya U6 promoter gene and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6127600A (en) * 1995-05-31 2000-10-03 Pioneer Hi-Bred International, Inc. Methods of increasing accumulation of essential amino acids in seeds
US6198021B1 (en) * 1993-05-28 2001-03-06 Long Ashton Research Station GA 20-oxidase gene sequences

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Publication number Priority date Publication date Assignee Title
US6288304B1 (en) * 1991-02-22 2001-09-11 Sembiosys Genetics Inc. Expression of somatotropin in plant seeds
US5965793A (en) 1995-09-20 1999-10-12 Monsanto Company, Inc. Strong early seed-specific gene regulatory region
US5981841A (en) * 1996-08-30 1999-11-09 Monsanto Company Early seed 5' regulatory sequence
ES2138924B1 (en) 1998-01-23 2000-10-01 Consejo Superior Investigacion PROMOTER AND REGULATORY SEQUENCES OF HA DS10 G1: A READ SUNFLOWER GENE EXCLUSIVELY EXPRESSED IN SEEDS SINCE THE RIPENING PHASE.
US7008664B1 (en) 1998-06-11 2006-03-07 E. I. Du Pont De Nemours And Company Method for improving the carcass quality of an animal
WO2001011061A2 (en) * 1999-08-04 2001-02-15 The University Of British Columbia Regulation of embryonic transcription in plants
DE60028053T2 (en) * 1999-08-27 2006-12-21 SemBioSys Genetics, Inc., Calgary SEED-SPECIFIC FLOAT PROMOTER (LINUM USITATISSIMUM)
CA2446258C (en) * 2001-05-04 2013-02-12 Renessen Llc Transgenic high tryptophan plants
CN1728937B (en) 2002-05-03 2011-03-30 孟山都技术公司 Temporal seed promoters for expressing genes in plants

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6198021B1 (en) * 1993-05-28 2001-03-06 Long Ashton Research Station GA 20-oxidase gene sequences
US6127600A (en) * 1995-05-31 2000-10-03 Pioneer Hi-Bred International, Inc. Methods of increasing accumulation of essential amino acids in seeds

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CALVO E.S. ET AL: "Cloning, mapping, and analyses of expression of the Em-like gene family in soybean [Glycine max (L). Merr.]", THEORETICAL AND APPLIED GENETICS, vol. 94, no. 8, 1997, pages 957 - 967, XP002978340 *
DATABASE GENBANK [online] 19 March 1998 (1998-03-19), XP002992852, Database accession no. (Y12089) *
DATABASE GENBANK [online] 27 April 1993 (1993-04-27), XP002992851, Database accession no. (M97285) *
DATABASE GENBANK [online] 27 April 1993 (1993-04-27), XP002992853, Database accession no. (K00821) *
See also references of EP1549132A4 *

Also Published As

Publication number Publication date
US20100077504A1 (en) 2010-03-25
US7615680B2 (en) 2009-11-10
US20120317679A1 (en) 2012-12-13
AU2003225283A1 (en) 2003-11-17
CA2483782C (en) 2013-07-16
CA2483782A1 (en) 2003-11-13
US8581044B2 (en) 2013-11-12
US20040016024A1 (en) 2004-01-22
CN1728937A (en) 2006-02-01
AU2003225283A8 (en) 2003-11-17
US20070180570A1 (en) 2007-08-02
US8193413B2 (en) 2012-06-05
US9303267B2 (en) 2016-04-05
WO2003092361A2 (en) 2003-11-13
EP1549132A2 (en) 2005-07-06
BR0309869A (en) 2005-04-26
CN1728937B (en) 2011-03-30
EP1549132A4 (en) 2005-12-14
US20140143911A1 (en) 2014-05-22
US7179959B2 (en) 2007-02-20

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