WO2004077007A3 - Detection, quantification and composition for cytochrome c released from mitochondria - Google Patents

Detection, quantification and composition for cytochrome c released from mitochondria Download PDF

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Publication number
WO2004077007A3
WO2004077007A3 PCT/BR2004/000022 BR2004000022W WO2004077007A3 WO 2004077007 A3 WO2004077007 A3 WO 2004077007A3 BR 2004000022 W BR2004000022 W BR 2004000022W WO 2004077007 A3 WO2004077007 A3 WO 2004077007A3
Authority
WO
WIPO (PCT)
Prior art keywords
cytochrome
released
mitochondria
detection
composition
Prior art date
Application number
PCT/BR2004/000022
Other languages
French (fr)
Other versions
WO2004077007A2 (en
Inventor
Claudia Barbosa Ladeira Campos
Ricardo G Cosso
Roger F Castilho
Hagai Rottenberg
Anibal E Vercesi
Original Assignee
Fapesp Fundacao De Amparo A Pe
Claudia Barbosa Ladeira Campos
Ricardo G Cosso
Roger F Castilho
Hagai Rottenberg
Anibal E Vercesi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fapesp Fundacao De Amparo A Pe, Claudia Barbosa Ladeira Campos, Ricardo G Cosso, Roger F Castilho, Hagai Rottenberg, Anibal E Vercesi filed Critical Fapesp Fundacao De Amparo A Pe
Publication of WO2004077007A2 publication Critical patent/WO2004077007A2/en
Publication of WO2004077007A3 publication Critical patent/WO2004077007A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G01N33/5735—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes co-enzymes or co-factors, e.g. NAD, ATP
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/795—Porphyrin- or corrin-ring-containing peptides
    • G01N2333/80—Cytochromes

Abstract

A method to quantify a cytochrome c released from mitochondria during cellul death comprising selective permeabilization of the plasma membrane and cytochrome c immunodetection is described. The present invention also regards a composition to detect cytochrome c released from mitochondria, a kit for detection of cytochrome c released from mitochondria, as well as the use of a composition for detection of cytochrome c released from mitochondria.
PCT/BR2004/000022 2003-02-28 2004-02-26 Detection, quantification and composition for cytochrome c released from mitochondria WO2004077007A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BRPI0300660A BRPI0300660B8 (en) 2003-02-28 2003-02-28 method to quantify mitochondrial release of cytochrome c and kit to detect mitochondrial release of cytochrome c
BRPI0300660-3 2003-02-28

Publications (2)

Publication Number Publication Date
WO2004077007A2 WO2004077007A2 (en) 2004-09-10
WO2004077007A3 true WO2004077007A3 (en) 2004-10-14

Family

ID=28679778

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BR2004/000022 WO2004077007A2 (en) 2003-02-28 2004-02-26 Detection, quantification and composition for cytochrome c released from mitochondria

Country Status (2)

Country Link
BR (1) BRPI0300660B8 (en)
WO (1) WO2004077007A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2970205B1 (en) 2013-03-14 2019-05-08 Tolero Pharmaceuticals, Inc. Jak2 and alk2 inhibitors and methods for their use
CN112512597A (en) 2018-07-26 2021-03-16 大日本住友制药肿瘤公司 Methods for treating diseases associated with aberrant ACVR1 expression and ACVR1 inhibitors useful therefor

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CASTEDO M. ET AL.: "Quantitation of mitochondrial alternations associated with apoptosis", J. IMMUNOL. METHODS, vol. 265, no. 1-2, 2002, pages 39 - 47, XP004366298 *
DUAN S. ET AL.: "Mitochondrial outer membrane permeability change and hypersensitivity to digitonin early in staurosporine-induced apoptosis", J. BIOL. CHEM., vol. 278, no. 2, 2003, pages 1346 - 1353, XP002904393 *
GOTTLIEB R.A., GRANVILLE D.J.: "Analyzing mitochondrial changes during apoptosis", METHODS, vol. 26, no. 4, 2002, pages 341 - 347, XP002904394 *
HAJEK P. ET AL.: "Rate-limiting step preceding cytochrome c release in cell primed fror Fas-mediated apoptosis revealed by analysis of cellular mosaicism of respiratory changes", J. BIOL. CHEM., vol. 276, no. 1, 2001, pages 606 - 615, XP002904392 *

Also Published As

Publication number Publication date
BRPI0300660B1 (en) 2017-02-21
BR0300660A (en) 2003-10-14
BRPI0300660B8 (en) 2021-07-27
WO2004077007A2 (en) 2004-09-10

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