WO2001041931A3 - Dispositifs microfluidiques multicouches pour reactions de substances a analyser - Google Patents

Dispositifs microfluidiques multicouches pour reactions de substances a analyser Download PDF

Info

Publication number
WO2001041931A3
WO2001041931A3 PCT/US2000/033499 US0033499W WO0141931A3 WO 2001041931 A3 WO2001041931 A3 WO 2001041931A3 US 0033499 W US0033499 W US 0033499W WO 0141931 A3 WO0141931 A3 WO 0141931A3
Authority
WO
WIPO (PCT)
Prior art keywords
devices
microfluidic devices
multilayered microfluidic
fabricated
reactions
Prior art date
Application number
PCT/US2000/033499
Other languages
English (en)
Other versions
WO2001041931A9 (fr
WO2001041931A2 (fr
Inventor
Cynthia G Briscoe
Jeremy W Burdon
Tony Chan
Barbara Foley
Piotr Grodzinski
George Hawkins
Rong-Fong Huang
Peter Kahn
Robert Marrero
Mark W Mcgarry
Todd Tuggle
Huinan Yu
Original Assignee
Motorola Inc
Cynthia G Briscoe
Jeremy W Burdon
Tony Chan
Barbara Foley
Piotr Grodzinski
George Hawkins
Huang Rong Fong
Peter Kahn
Robert Marrero
Mark W Mcgarry
Todd Tuggle
Huinan Yu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/458,534 external-priority patent/US6642046B1/en
Priority claimed from US09/460,283 external-priority patent/US6527890B1/en
Application filed by Motorola Inc, Cynthia G Briscoe, Jeremy W Burdon, Tony Chan, Barbara Foley, Piotr Grodzinski, George Hawkins, Huang Rong Fong, Peter Kahn, Robert Marrero, Mark W Mcgarry, Todd Tuggle, Huinan Yu filed Critical Motorola Inc
Priority to EP00984156A priority Critical patent/EP1237655A2/fr
Priority to CA002393690A priority patent/CA2393690A1/fr
Priority to US10/149,318 priority patent/US20040043479A1/en
Priority to JP2001543266A priority patent/JP2003517591A/ja
Priority to AU20827/01A priority patent/AU2082701A/en
Publication of WO2001041931A2 publication Critical patent/WO2001041931A2/fr
Publication of WO2001041931A3 publication Critical patent/WO2001041931A3/fr
Publication of WO2001041931A9 publication Critical patent/WO2001041931A9/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G01N30/6069—Construction of the column body with compartments or bed substructure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6095—Micromachined or nanomachined, e.g. micro- or nanosize
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00495—Means for heating or cooling the reaction vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00783—Laminate assemblies, i.e. the reactor comprising a stack of plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00819—Materials of construction
    • B01J2219/00824—Ceramic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00851—Additional features
    • B01J2219/00858—Aspects relating to the size of the reactor
    • B01J2219/0086—Dimensions of the flow channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00851—Additional features
    • B01J2219/00858—Aspects relating to the size of the reactor
    • B01J2219/00862—Dimensions of the reaction cavity itself
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00873—Heat exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00891—Feeding or evacuation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00905—Separation
    • B01J2219/00916—Separation by chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1883—Means for temperature control using thermal insulation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0418—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electro-osmotic flow [EOF]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F28—HEAT EXCHANGE IN GENERAL
    • F28F—DETAILS OF HEAT-EXCHANGE AND HEAT-TRANSFER APPARATUS, OF GENERAL APPLICATION
    • F28F2260/00—Heat exchangers or heat exchange elements having special size, e.g. microstructures
    • F28F2260/02—Heat exchangers or heat exchange elements having special size, e.g. microstructures having microchannels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N2030/0095—Separation specially adapted for use outside laboratory, e.g. field sampling, portable equipments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/025—Gas chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/524—Physical parameters structural properties
    • G01N2030/528—Monolithic sorbent material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00158—Elements containing microarrays, i.e. "biochip"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks

Abstract

L'invention concerne des procédés et des appareils servant à réaliser des analyses, notamment des dispositifs microfluidiques. Selon des aspects préférés, les dispositifs sont fabriqués au moyen d'une technique multicouches de céramique destinée à constituer des dispositifs dans lesquels on peut réaliser des réactions moléculaires parallèles, régulées indépendamment, telles que des réactions d'amplification d'acides nucléiques comprenant la réaction en chaîne de la polymérase (PCR). En outre, les dispositifs peuvent inclure et comprendre des micro chromatographes en phase gazeuse, fabriqués de manière similaire à partir de céramique.
PCT/US2000/033499 1999-12-09 2000-12-11 Dispositifs microfluidiques multicouches pour reactions de substances a analyser WO2001041931A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP00984156A EP1237655A2 (fr) 1999-12-09 2000-12-11 Dispositifs microfluidiques multicouches pour reactions de substances a analyser
CA002393690A CA2393690A1 (fr) 1999-12-09 2000-12-11 Dispositifs microfluidiques multicouches pour reactions de substances a analyser
US10/149,318 US20040043479A1 (en) 2000-12-11 2000-12-11 Multilayerd microfluidic devices for analyte reactions
JP2001543266A JP2003517591A (ja) 1999-12-09 2000-12-11 分析試料の反応を行うための多層微量流体デバイス
AU20827/01A AU2082701A (en) 1999-12-09 2000-12-11 Multilayered microfluidic devices for analyte reactions

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US09/458,534 US6642046B1 (en) 1999-12-09 1999-12-09 Method and apparatus for performing biological reactions on a substrate surface
US09/460,281 1999-12-09
US09/460,283 US6527890B1 (en) 1998-10-09 1999-12-09 Multilayered ceramic micro-gas chromatograph and method for making the same
US09/458,534 1999-12-09
US09/460,281 US6544734B1 (en) 1998-10-09 1999-12-09 Multilayered microfluidic DNA analysis system and method
US09/460,283 1999-12-09
US46632599A 1999-12-17 1999-12-17
US09/466,325 1999-12-17

Publications (3)

Publication Number Publication Date
WO2001041931A2 WO2001041931A2 (fr) 2001-06-14
WO2001041931A3 true WO2001041931A3 (fr) 2002-01-03
WO2001041931A9 WO2001041931A9 (fr) 2002-05-30

Family

ID=27504051

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/033499 WO2001041931A2 (fr) 1999-12-09 2000-12-11 Dispositifs microfluidiques multicouches pour reactions de substances a analyser

Country Status (5)

Country Link
EP (1) EP1237655A2 (fr)
JP (1) JP2003517591A (fr)
AU (1) AU2082701A (fr)
CA (1) CA2393690A1 (fr)
WO (1) WO2001041931A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106164651A (zh) * 2014-01-29 2016-11-23 Bg研究有限公司 用于热循环生物化学操作的装置和方法

Families Citing this family (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6572830B1 (en) 1998-10-09 2003-06-03 Motorola, Inc. Integrated multilayered microfludic devices and methods for making the same
US6637463B1 (en) 1998-10-13 2003-10-28 Biomicro Systems, Inc. Multi-channel microfluidic system design with balanced fluid flow distribution
US6601613B2 (en) 1998-10-13 2003-08-05 Biomicro Systems, Inc. Fluid circuit components based upon passive fluid dynamics
US6591852B1 (en) 1998-10-13 2003-07-15 Biomicro Systems, Inc. Fluid circuit components based upon passive fluid dynamics
WO2002025268A1 (fr) * 2000-09-25 2002-03-28 Gl Sciences Incorporated Procede et dispositif servant a recueillir et a concentrer un echantillon
US6787339B1 (en) 2000-10-02 2004-09-07 Motorola, Inc. Microfluidic devices having embedded metal conductors and methods of fabricating said devices
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US6852287B2 (en) * 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
DE60109292T2 (de) * 2001-10-03 2006-04-13 C.S.E.M. Centre Suisse D'electronique Et De Microtechnique S.A. Kombinierte Vorrichtung zur Behandlung biologischer Mikromatrizen
US7338760B2 (en) * 2001-10-26 2008-03-04 Ntu Ventures Private Limited Sample preparation integrated chip
US20030138819A1 (en) * 2001-10-26 2003-07-24 Haiqing Gong Method for detecting disease
KR100459896B1 (ko) * 2002-03-06 2004-12-04 삼성전자주식회사 Ｐｃｒ 칩을 구동하기 위한 온도 제어 방법 및 그 장치
KR100450818B1 (ko) * 2002-03-09 2004-10-01 삼성전자주식회사 다챔버 ｐｃｒ 칩
JP3891131B2 (ja) * 2002-03-29 2007-03-14 カシオ計算機株式会社 化学反応装置及び電源システム
TW592830B (en) 2002-03-29 2004-06-21 Casio Computer Co Ltd Chemical reaction apparatus and power supply system
WO2003106032A1 (fr) * 2002-06-14 2003-12-24 Axaron Bioscience Ag Chambre d'hybridation
US20030235521A1 (en) * 2002-06-21 2003-12-25 Shea Laurence R. Array assay devices and methods of using the same
JP4380977B2 (ja) * 2002-10-28 2009-12-09 東洋紡績株式会社 表面プラズモン共鳴測定用フローセル
CN1217188C (zh) * 2002-12-05 2005-08-31 清华大学 微型气相色谱柱、气相色谱***以及在样品中分析组分的方法
US6955777B2 (en) * 2003-01-07 2005-10-18 International Business Machines Corporation Method of forming a plate for dispensing chemicals
EP1641563B1 (fr) * 2003-05-23 2018-08-29 Bio-Rad Laboratories, Inc. Controle de temperature localise pour ensemble spatial de milieux reactionnels
JP4758891B2 (ja) 2003-06-06 2011-08-31 マイクロニクス， インコーポレイテッド 微小流体デバイス上の加熱、冷却および熱サイクリングのためのシステムおよび方法
US7648835B2 (en) 2003-06-06 2010-01-19 Micronics, Inc. System and method for heating, cooling and heat cycling on microfluidic device
JP2005024316A (ja) * 2003-06-30 2005-01-27 Kyocera Corp マイクロ化学チップおよびその製造方法
WO2005011867A2 (fr) 2003-07-31 2005-02-10 Handylab, Inc. Traitement d'echantillons contenant des particules
US7341834B2 (en) 2003-12-15 2008-03-11 Geneohn Sciences, Inc. Multiplexed electrochemical detection system and method
GB2416030B (en) * 2004-01-28 2008-07-23 Norchip As A diagnostic system for carrying out a nucleic acid sequence amplification and detection process
JP2007523351A (ja) * 2004-02-17 2007-08-16 カリフォルニア インスティチュート オブ テクノロジー オンチップ温度制御型液体クロマトグラフィー方法およびデバイス
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US7887750B2 (en) * 2004-05-05 2011-02-15 Bayer Healthcare Llc Analytical systems, devices, and cartridges therefor
US20090191096A1 (en) * 2004-07-29 2009-07-30 Kyocera Corporation Microchemical Chip
US7645423B2 (en) 2004-08-20 2010-01-12 International Business Machines Corporation Optical micro plugs for multichannel and multilayer pharmaceutical device
JP4574369B2 (ja) * 2004-08-26 2010-11-04 京セラ株式会社 配線基板およびその製造方法
US7402616B2 (en) 2004-09-30 2008-07-22 Lifescan, Inc. Fusible conductive ink for use in manufacturing microfluidic analytical systems
JP2006133220A (ja) * 2004-10-04 2006-05-25 Fuji Photo Film Co Ltd 試料供給方法および試料供給装置
WO2006065598A2 (fr) * 2004-12-13 2006-06-22 Geneohm Sciences, Inc. Cartouches fluidiques utilisees dans la detection electrochimique de l'adn
DE102005004075B4 (de) * 2005-01-28 2008-04-03 Umicore Ag & Co. Kg Keramischer Mikroreaktor
JP2006223126A (ja) * 2005-02-15 2006-08-31 Shimadzu Corp 反応容器、遺伝子多型検出方法及び装置、並びに診断方法及び装置
JP4714475B2 (ja) * 2005-02-15 2011-06-29 株式会社島津製作所 反応容器、遺伝子多型検出方法及び装置
EP1887363A4 (fr) * 2005-04-01 2012-08-22 Konica Minolta Med & Graphic Micro système d analyse globale, puce d examen et procédé d examen
JP4917765B2 (ja) * 2005-06-17 2012-04-18 凸版印刷株式会社 Ｐｃｒ反応用容器
JP4870946B2 (ja) * 2005-06-17 2012-02-08 凸版印刷株式会社 反応容器および反応方法
DE102005029809B4 (de) * 2005-06-27 2007-04-26 Siemens Ag Vorrichtung und Verfahren zur Aufbereitung einer Probe für eine Analyse und Vorrichtung und Verfahren zur Analyse einer Probe
DE102005031920B4 (de) * 2005-07-07 2007-12-20 Isotopen Technologien München AG Vorrichtung und Verfahren zur Herstellung einer geringen Menge einer radioaktiven Substanzverbindung
JP2007064793A (ja) * 2005-08-31 2007-03-15 Thk Co Ltd Ｄｎａマイクロアレイチップに対する溶液供給装置および溶液供給方法
EP1940543B1 (fr) 2005-09-29 2012-03-07 Siemens Medical Solutions USA, Inc. Puce microfluidique capable de synthetiser des molecules radiomarquees a une echelle adaptee a l'imagerie humaine par tomographie par emission de positrons
JP2007132704A (ja) * 2005-11-08 2007-05-31 Tosoh Quartz Corp ガラス製マイクロチップベースプレート、ガラス製マイクロチップベースプレートの製造方法及びマイクロチップ
JP5009532B2 (ja) * 2006-01-20 2012-08-22 凸版印刷株式会社 断熱容器及び反応容器ユニット
JP2007189960A (ja) * 2006-01-20 2007-08-02 Toppan Printing Co Ltd 反応容器
JP2007189962A (ja) * 2006-01-20 2007-08-02 Toppan Printing Co Ltd 反応容器
JP2007189964A (ja) * 2006-01-20 2007-08-02 Toppan Printing Co Ltd 検査基板
US7740806B2 (en) 2006-02-28 2010-06-22 International Business Machines Corporation Ceramic microarray spotting device for bioassay printing
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
DK3088083T3 (en) 2006-03-24 2018-11-26 Handylab Inc Method of carrying out PCR down a multi-track cartridge
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
AU2007254191B2 (en) * 2006-05-16 2013-01-10 Fluidigm Corporation PCR-free sample preparation and detection systems for high speed biologic analysis and identification
GB0610432D0 (en) * 2006-05-26 2006-07-05 B G Res Ltd Performance issues in use of vessels for biological applications
US8173071B2 (en) 2006-08-29 2012-05-08 International Business Machines Corporation Micro-fluidic test apparatus and method
EP2091647A2 (fr) 2006-11-14 2009-08-26 Handylab, Inc. Système microfluidique utilisé pour amplifier et détecter des polynucléotides en parallèle
WO2008091694A2 (fr) 2007-01-23 2008-07-31 Siemens Medical Solutions Usa, Inc. Système microfluidique entièrement automatisé pour réaliser la synthèse de biomarqueurs radiomarqués pour tomographie par émission de positons
JP2008180677A (ja) 2007-01-26 2008-08-07 Konica Minolta Medical & Graphic Inc マイクロチップ検査システム、マイクロチップ検査装置及びプログラム
US20080241844A1 (en) * 2007-02-06 2008-10-02 Network Biosystems, Inc. Devices and Methods for the Performance of Miniaturized In Vitro Assays
CN101541962A (zh) * 2007-03-23 2009-09-23 株式会社东芝 核酸检测盒以及核酸检测装置
CA2682734C (fr) 2007-04-04 2017-11-07 Network Biosystems, Inc. Plate-formes microfluidiques en plastique de detection et de separation
US8071035B2 (en) 2007-04-12 2011-12-06 Siemens Medical Solutions Usa, Inc. Microfluidic radiosynthesis system for positron emission tomography biomarkers
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8324372B2 (en) 2007-07-13 2012-12-04 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
WO2009018473A1 (fr) 2007-07-31 2009-02-05 Micronics, Inc. Système de récupération d'écouvillon sanitaire, dispositif d'analyse microfluidique et procédés pour des analyses de diagnostic
GB0715170D0 (en) 2007-08-03 2007-09-12 Enigma Diagnostics Ltd Reaction vessel
GB0715171D0 (en) 2007-08-03 2007-09-12 Enigma Diagnostics Ltd Sample processor
US20120082985A1 (en) * 2007-08-09 2012-04-05 Frederic Zenhausern Sensing And Identifying Biological Samples On Microfluidic Devices
PE20090965A1 (es) 2007-10-12 2009-07-13 Bigtec Private Ltd Micro chip
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
EP2401398B1 (fr) * 2009-02-27 2017-02-08 Koninklijke Philips N.V. Sélection génomique et séquençage utilisant des microporteurs codés
JP2010213649A (ja) * 2009-03-18 2010-09-30 Seiko Epson Corp 生体試料反応容器及び生体試料反応方法
US9114395B2 (en) 2009-04-15 2015-08-25 Koninklijke Philips N.V. Cartridge for an amplification process
EP2443254A2 (fr) 2009-06-15 2012-04-25 NetBio, Inc. Procédés perfectionnés pour une quantification d'adn à des fins médicolégales
JP5306134B2 (ja) * 2009-09-28 2013-10-02 京セラ株式会社 送液装置
US8685749B2 (en) * 2009-12-02 2014-04-01 Whatman International Limited Methods and systems for processing samples on porous substrates
JP5935696B2 (ja) * 2010-11-22 2016-06-15 国立大学法人北海道大学 ポータブルな液体クロマトグラフ及び液体クロマトグラフィー
BR112013026451B1 (pt) 2011-04-15 2021-02-09 Becton, Dickinson And Company sistema e método para realizar ensaios de diagnóstico molecular em várias amostras em paralelo e simultaneamente amplificação em tempo real em pluralidade de câmaras de reação de amplificação
NZ735051A (en) 2011-05-12 2022-11-25 Netbio Inc Methods and compositions for rapid multiplex amplification of str loci
CN103403541A (zh) * 2011-08-04 2013-11-20 株式会社堀场Stec 平板型毛细管柱、毛细管柱单元以及使用其的色谱仪
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
JP6117217B2 (ja) 2011-09-30 2017-04-19 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ ユニット化された試薬ストリップ
EP2773892B1 (fr) 2011-11-04 2020-10-07 Handylab, Inc. Dispositif de préparation d'échantillons de polynucléotides
US10822644B2 (en) 2012-02-03 2020-11-03 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
WO2014127806A1 (fr) * 2013-02-19 2014-08-28 Daiken Medical Co., Ltd. Kit d'amplification d'adn
WO2014127805A1 (fr) * 2013-02-19 2014-08-28 Daiken Medical Co., Ltd. Méthode de diagnostic de pathogène
WO2014127804A1 (fr) * 2013-02-19 2014-08-28 Daiken Medical Co., Ltd. Système de diagnostic d'agents pathogènes
WO2015106356A1 (fr) * 2014-01-17 2015-07-23 Coastal Genomics Inc. Cassettes à utiliser lors d'analyses électrophorétiques parallèles automatisées et leurs procédés de fabrication et d'utilisation
US9845499B2 (en) * 2016-04-04 2017-12-19 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification
US11285478B2 (en) 2016-04-04 2022-03-29 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification
WO2018094091A1 (fr) 2016-11-17 2018-05-24 Combinati Incorporated Procédés et systèmes d'analyse et de quantification d'acide nucléique
CN109298734A (zh) * 2018-09-07 2019-02-01 上海出入境检验检疫局工业品与原材料检测技术中心 智能温控检测设备的控制方法
CN109736778B (zh) * 2019-03-01 2023-11-21 中国石油大学(北京) 井眼水化变形红外监测装置及方法
CN113000079B (zh) * 2020-06-02 2023-09-22 山东大学 一种重金属离子检测电化学微流控传感芯片及其制备方法
US20210402396A1 (en) * 2020-06-30 2021-12-30 Samsung Electronics Co., Ltd. Microfluidic chip, and apparatus and method for detecting biomolecules
CN117836043A (zh) * 2021-08-30 2024-04-05 株式会社大赛璐 微流体设备及其制造方法
WO2023229010A1 (fr) * 2022-05-26 2023-11-30 キッコーマン株式会社 Procede et dispositif de detection de micro-organismes

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5589136A (en) * 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
EP0870541A2 (fr) * 1997-04-11 1998-10-14 Eastman Kodak Company Installation micro-chimique en céramique
WO1998050154A1 (fr) * 1997-05-08 1998-11-12 University Of Minnesota Systeme de test genetique a micropuce integree
US5939312A (en) * 1995-05-24 1999-08-17 Biometra Biomedizinische Analytik Gmbh Miniaturized multi-chamber thermocycler

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5939312A (en) * 1995-05-24 1999-08-17 Biometra Biomedizinische Analytik Gmbh Miniaturized multi-chamber thermocycler
US5589136A (en) * 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
EP0870541A2 (fr) * 1997-04-11 1998-10-14 Eastman Kodak Company Installation micro-chimique en céramique
WO1998050154A1 (fr) * 1997-05-08 1998-11-12 University Of Minnesota Systeme de test genetique a micropuce integree

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106164651A (zh) * 2014-01-29 2016-11-23 Bg研究有限公司 用于热循环生物化学操作的装置和方法

Also Published As

Publication number Publication date
EP1237655A2 (fr) 2002-09-11
WO2001041931A9 (fr) 2002-05-30
CA2393690A1 (fr) 2001-06-14
WO2001041931A2 (fr) 2001-06-14
JP2003517591A (ja) 2003-05-27
AU2082701A (en) 2001-06-18

Similar Documents

Publication Publication Date Title
WO2001041931A3 (fr) Dispositifs microfluidiques multicouches pour reactions de substances a analyser
WO2007096702A3 (fr) Procédés d'amplification multiple par recombinase-polymérase
WO2005118773A3 (fr) Appareil et procedes pour effectuer des analyses multiplex
WO2002036832A3 (fr) Atropisomeres de teintures fluorescentes de xanthene asymetrique; methodes de sequençage d'adn et d'analyse de fragments d'adn
WO2006047777A3 (fr) Reactions d'amplification d'acide nucleique a stades multiples en systeme ferme
WO2003056030A3 (fr) Procedes et systemes pour le sequençage d'acides nucleiques
WO2005068628A3 (fr) Procedes d'isolation d'un acide nucleique et trousses utilisant un materiau en phase solide
WO2003004690A3 (fr) Methode utilisant un filtre poreux pour isoler en parallele des micro-reactions chimiques independantes
SG145758A1 (en) Apparatus for processing a fluid sample
AU2261001A (en) Helicobacter pylori antigens in blood
EP1256630A3 (fr) Réaction de chaíne polymérase séléctive de l'ADN de laquelle les bases de séquence sont totallement inconnus
WO2007024798A3 (fr) Dispositif, systeme et procede utilisant des volumes discrets de fluides non miscibles
WO2005116257A3 (fr) Sequences courtes specifiques repetees en tandem et methodes d'utilisation
ATE395136T1 (de) Vorrichtungen zur durchfuehrung von miniaturisierten in vitro amplifizierungsassays
HK1127091A1 (en) Droplet-based pyrosequencing
DK2080813T3 (da) Nukleinsyreligander, som binder til og inhiberer DNA-polymeraser
IL131966A0 (en) An in vitro method for construction of a dna library
ATE400663T1 (de) Rekombinase-polymerase-amplifikation
EP1350841A3 (fr) ADN polymérase thermostable ou thermoactif ayant une activité 3' - 5' exonucléase atténuée
DK1676117T3 (da) Simplificeret forarbejdning af væv
WO2003040395A3 (fr) Nucleotides universels pour analyse d'acides nucleiques
WO2005054431A3 (fr) Procede permettant d'isoler des micro-reactions chimiques paralleles independantes au moyen d'un filtre poreux
EP2149614A8 (fr) Amorces pour la détection de Mycobacterium tuberculosis
AU2470395A (en) Materials and methods for the detection of (mycobacterium tuberculosis)
WO2000050564A3 (fr) Procede d'extraction d'adn de prelevements seches

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

AK Designated states

Kind code of ref document: C2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

COP Corrected version of pamphlet

Free format text: PAGES 1/29-29/29, DRAWINGS, REPLACED BY NEW PAGES 1/26-26/26; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2001 543266

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 2393690

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 20827/01

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2000984156

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2000984156

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 10149318

Country of ref document: US

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWW Wipo information: withdrawn in national office

Ref document number: 2000984156

Country of ref document: EP