WO1997046673A3 - Inhibition of hiv-1 replication by antisense rna expression - Google Patents

Inhibition of hiv-1 replication by antisense rna expression Download PDF

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Publication number
WO1997046673A3
WO1997046673A3 PCT/EP1997/002952 EP9702952W WO9746673A3 WO 1997046673 A3 WO1997046673 A3 WO 1997046673A3 EP 9702952 W EP9702952 W EP 9702952W WO 9746673 A3 WO9746673 A3 WO 9746673A3
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WO
WIPO (PCT)
Prior art keywords
hiv
replication
inhibition
antisense rna
rna expression
Prior art date
Application number
PCT/EP1997/002952
Other languages
French (fr)
Other versions
WO1997046673A2 (en
Inventor
Ernst Boehnlein
Sonia Escaich
Heini Ilves
Gabor Veres
Original Assignee
Ciba Geigy Ag
Systemix Inc
Ernst Boehnlein
Sonia Escaich
Heini Ilves
Gabor Veres
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy Ag, Systemix Inc, Ernst Boehnlein, Sonia Escaich, Heini Ilves, Gabor Veres filed Critical Ciba Geigy Ag
Priority to NZ333190A priority Critical patent/NZ333190A/en
Priority to JP10500237A priority patent/JP2001502884A/en
Priority to EP97928151A priority patent/EP0914423A2/en
Priority to AU32558/97A priority patent/AU717233B2/en
Priority to IL12703897A priority patent/IL127038A0/en
Priority to US09/194,300 priority patent/US6776986B1/en
Publication of WO1997046673A2 publication Critical patent/WO1997046673A2/en
Publication of WO1997046673A3 publication Critical patent/WO1997046673A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C12N15/1132—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses against retroviridae, e.g. HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
    • C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Public Health (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • AIDS & HIV (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Novel antisense sequences to the unspliced or single spliced portions of mRNA transcript from HIV-1 provirus, optionally co-expressed with an inhibitory transdominant mutant HIV-1 protein, are found to be useful in the treatment of HIV-1 infection.
PCT/EP1997/002952 1996-06-06 1997-06-06 Inhibition of hiv-1 replication by antisense rna expression WO1997046673A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
NZ333190A NZ333190A (en) 1996-06-06 1997-06-06 Inhibition of HIV-1 replication by antisense RNA expression using transduced hematopoietic cells
JP10500237A JP2001502884A (en) 1996-06-06 1997-06-06 Inhibition of HIV-1 replication by antisense RNA expression
EP97928151A EP0914423A2 (en) 1996-06-06 1997-06-06 Inhibition of hiv-1 replication by antisense rna expression
AU32558/97A AU717233B2 (en) 1996-06-06 1997-06-06 Inhibition of HIV-1 replication by antisense RNA expression
IL12703897A IL127038A0 (en) 1996-06-06 1997-06-06 Inhibition of hiv-1 replication by antisense rna expression
US09/194,300 US6776986B1 (en) 1996-06-06 1997-06-06 Inhibition of HIV-1 replication by antisense RNA expression

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1923296P 1996-06-06 1996-06-06
US60/019,232 1996-06-06

Publications (2)

Publication Number Publication Date
WO1997046673A2 WO1997046673A2 (en) 1997-12-11
WO1997046673A3 true WO1997046673A3 (en) 1998-01-15

Family

ID=21792127

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1997/002952 WO1997046673A2 (en) 1996-06-06 1997-06-06 Inhibition of hiv-1 replication by antisense rna expression

Country Status (7)

Country Link
EP (1) EP0914423A2 (en)
JP (1) JP2001502884A (en)
AU (1) AU717233B2 (en)
CA (1) CA2254819A1 (en)
IL (1) IL127038A0 (en)
NZ (1) NZ333190A (en)
WO (1) WO1997046673A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6776986B1 (en) 1996-06-06 2004-08-17 Novartis Ag Inhibition of HIV-1 replication by antisense RNA expression
AU7104200A (en) * 2000-09-01 2002-03-22 Gen Probe Inc Amplification of hiv-1 sequences for detection of sequences associated with drug-resistance mutations
US6582920B2 (en) 2000-09-01 2003-06-24 Gen-Probe Incorporated Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations
JP5461459B2 (en) * 2011-03-01 2014-04-02 ジェン−プローブ・インコーポレーテッド Amplification of HIV-1 sequences for detection of sequences associated with drug resistance mutations
JP5461460B2 (en) * 2011-03-01 2014-04-02 ジェン−プローブ・インコーポレーテッド Amplification of HIV-1 sequences for detection of sequences associated with drug resistance mutations
JP2012105648A (en) * 2011-12-12 2012-06-07 Gen Probe Inc Amplification of hiv-1 sequence for sequence detection associated with drug resistant mutation
US9932364B2 (en) 2013-08-26 2018-04-03 The Royal Institution For The Advancement Of Learning/Mcgill University Antisense-based small RNA agents targeting the Gag open reading frame of HIV-1 RNA

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990014427A2 (en) * 1989-05-25 1990-11-29 Sandoz Ltd Multivalent repressor of gene function
EP0598935A1 (en) * 1992-11-24 1994-06-01 Bayer Ag Expression vectors and their use to produce HIV-resistant human cells for therapeutic use
EP0612844A2 (en) * 1993-02-25 1994-08-31 Ortho Pharmaceutical Corporation Expression constructs containing HIV inhibitory antisense and other nucleotide sequences, retroviral vectors and recombinant retroviruses containing same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990014427A2 (en) * 1989-05-25 1990-11-29 Sandoz Ltd Multivalent repressor of gene function
EP0598935A1 (en) * 1992-11-24 1994-06-01 Bayer Ag Expression vectors and their use to produce HIV-resistant human cells for therapeutic use
EP0612844A2 (en) * 1993-02-25 1994-08-31 Ortho Pharmaceutical Corporation Expression constructs containing HIV inhibitory antisense and other nucleotide sequences, retroviral vectors and recombinant retroviruses containing same

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
RENNEISEN K ET AL: "INHIBITION OF EXPRESSION OF HUMAN IMMUNODEFICIENCY VIRUS-1 IN VITRO BY ANTIBODY-TARGETED LIPOSOMES CONTAINING ANTISENSE RNA TO THE ENV REGION", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 265, no. 27, 25 September 1990 (1990-09-25), pages 16337 - 16342, XP000148392 *
VANDENDRIESSCHE, T. ET AL.: "Inhibition of clinical human immunodeficiency virus (HIV) type 1 isolates in primary CD4+ T lymphocytes by retroviral vectors expressing anti-HIV genes", JOURNAL OF VIROLOGY., vol. 69, July 1995 (1995-07-01), AMERICAN SOCIETY FOR MICROBIOLOGY US, pages 4045 - 4052, XP002046632 *
WOFFENDIN, C. ET AL.: "Expression of a protective gene prolongs survival of T cells in human immunodeficiency virus-infected patients", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 93, April 1996 (1996-04-01), WASHINGTON US, pages 2889 - 2894, XP002046633 *

Also Published As

Publication number Publication date
NZ333190A (en) 2001-03-30
EP0914423A2 (en) 1999-05-12
JP2001502884A (en) 2001-03-06
CA2254819A1 (en) 1997-12-11
WO1997046673A2 (en) 1997-12-11
AU717233B2 (en) 2000-03-23
AU3255897A (en) 1998-01-05
IL127038A0 (en) 1999-09-22

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