SU611598A3 - Способ подсчета лейкоцитов - Google Patents
Способ подсчета лейкоцитовInfo
- Publication number
- SU611598A3 SU611598A3 SU711711809A SU1711809A SU611598A3 SU 611598 A3 SU611598 A3 SU 611598A3 SU 711711809 A SU711711809 A SU 711711809A SU 1711809 A SU1711809 A SU 1711809A SU 611598 A3 SU611598 A3 SU 611598A3
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- solution
- cells
- staining
- reagent
- hemolysis
- Prior art date
Links
- 238000000034 method Methods 0.000 title description 4
- 239000000243 solution Substances 0.000 description 15
- 210000004027 cell Anatomy 0.000 description 11
- 239000003153 chemical reaction reagent Substances 0.000 description 11
- 238000010186 staining Methods 0.000 description 11
- 210000000265 leukocyte Anatomy 0.000 description 9
- 206010018910 Haemolysis Diseases 0.000 description 8
- 230000008588 hemolysis Effects 0.000 description 8
- 239000000758 substrate Substances 0.000 description 7
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 230000000093 cytochemical effect Effects 0.000 description 5
- 150000002148 esters Chemical class 0.000 description 5
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 4
- 239000000975 dye Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- XIVJNRXPRQKFRZ-UHFFFAOYSA-N naphthalen-1-yl butanoate Chemical compound C1=CC=C2C(OC(=O)CCC)=CC=CC2=C1 XIVJNRXPRQKFRZ-UHFFFAOYSA-N 0.000 description 4
- 210000000440 neutrophil Anatomy 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- ZTQSAGDEMFDKMZ-UHFFFAOYSA-N Butyraldehyde Chemical compound CCCC=O ZTQSAGDEMFDKMZ-UHFFFAOYSA-N 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000003992 Peroxidases Human genes 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 210000000601 blood cell Anatomy 0.000 description 3
- 210000001124 body fluid Anatomy 0.000 description 3
- 239000010839 body fluid Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 3
- 210000001616 monocyte Anatomy 0.000 description 3
- 230000001376 precipitating effect Effects 0.000 description 3
- PRPINYUDVPFIRX-UHFFFAOYSA-M 1-naphthaleneacetate Chemical compound C1=CC=C2C(CC(=O)[O-])=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-M 0.000 description 2
- DDYBAABYDNWVPI-UHFFFAOYSA-N 4-chloronaphthalene-1-carbaldehyde Chemical compound C1=CC=C2C(Cl)=CC=C(C=O)C2=C1 DDYBAABYDNWVPI-UHFFFAOYSA-N 0.000 description 2
- 239000005725 8-Hydroxyquinoline Substances 0.000 description 2
- IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical compound CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 description 2
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 2
- 108010054147 Hemoglobins Proteins 0.000 description 2
- JBOPQACSHPPKEP-UHFFFAOYSA-N Indoxyl acetate Chemical compound C1=CC=C2C(OC(=O)C)=CNC2=C1 JBOPQACSHPPKEP-UHFFFAOYSA-N 0.000 description 2
- NBBJYMSMWIIQGU-UHFFFAOYSA-N Propionic aldehyde Chemical compound CCC=O NBBJYMSMWIIQGU-UHFFFAOYSA-N 0.000 description 2
- 150000001299 aldehydes Chemical class 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000003979 eosinophil Anatomy 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 239000000834 fixative Substances 0.000 description 2
- 230000002949 hemolytic effect Effects 0.000 description 2
- PCKPVGOLPKLUHR-UHFFFAOYSA-N indoxyl Chemical group C1=CC=C2C(O)=CNC2=C1 PCKPVGOLPKLUHR-UHFFFAOYSA-N 0.000 description 2
- -1 naphthyl chloroacetate Chemical compound 0.000 description 2
- 229960003540 oxyquinoline Drugs 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 150000002978 peroxides Chemical class 0.000 description 2
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 2
- RRQYJINTUHWNHW-UHFFFAOYSA-N 1-ethoxy-2-(2-ethoxyethoxy)ethane Chemical compound CCOCCOCCOCC RRQYJINTUHWNHW-UHFFFAOYSA-N 0.000 description 1
- KJCVRFUGPWSIIH-UHFFFAOYSA-N 1-naphthol Chemical compound C1=CC=C2C(O)=CC=CC2=C1 KJCVRFUGPWSIIH-UHFFFAOYSA-N 0.000 description 1
- DPUSLOQBAPOARC-UHFFFAOYSA-N 1h-indol-3-yl butanoate Chemical compound C1=CC=C2C(OC(=O)CCC)=CNC2=C1 DPUSLOQBAPOARC-UHFFFAOYSA-N 0.000 description 1
- 206010053567 Coagulopathies Diseases 0.000 description 1
- 241001352366 Leucoma Species 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940089960 chloroacetate Drugs 0.000 description 1
- 230000035602 clotting Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000001268 conjugating effect Effects 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 150000002170 ethers Chemical class 0.000 description 1
- 239000008098 formaldehyde solution Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical class CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 150000001451 organic peroxides Chemical class 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000001044 red dye Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- SWGJCIMEBVHMTA-UHFFFAOYSA-K trisodium;6-oxido-4-sulfo-5-[(4-sulfonatonaphthalen-1-yl)diazenyl]naphthalene-2-sulfonate Chemical compound [Na+].[Na+].[Na+].C1=CC=C2C(N=NC3=C4C(=CC(=CC4=CC=C3O)S([O-])(=O)=O)S([O-])(=O)=O)=CC=C(S([O-])(=O)=O)C2=C1 SWGJCIMEBVHMTA-UHFFFAOYSA-K 0.000 description 1
- HGBOYTHUEUWSSQ-UHFFFAOYSA-N valeric aldehyde Natural products CCCCC=O HGBOYTHUEUWSSQ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/808—Optical sensing apparatus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/11—Automated chemical analysis
- Y10T436/117497—Automated chemical analysis with a continuously flowing sample or carrier stream
- Y10T436/118339—Automated chemical analysis with a continuously flowing sample or carrier stream with formation of a segmented stream
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Ecology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US8533370A | 1970-10-30 | 1970-10-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
SU611598A3 true SU611598A3 (ru) | 1978-06-15 |
Family
ID=22190904
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU711711809A SU611598A3 (ru) | 1970-10-30 | 1971-10-29 | Способ подсчета лейкоцитов |
Country Status (12)
Country | Link |
---|---|
US (1) | US3741875A (de) |
JP (2) | JPS576932B1 (de) |
AU (1) | AU460469B2 (de) |
BE (1) | BE774311A (de) |
CA (1) | CA960947A (de) |
DE (1) | DE2153479B2 (de) |
FR (1) | FR2113336A5 (de) |
GB (1) | GB1331568A (de) |
IT (1) | IT961518B (de) |
NL (1) | NL172463C (de) |
SE (1) | SE372417B (de) |
SU (1) | SU611598A3 (de) |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2125699B2 (de) * | 1971-05-25 | 1973-05-17 | Phywe AG, 3400 Gottingen | Praeparation von zellen zur messung von dns |
US4049381A (en) * | 1976-03-23 | 1977-09-20 | Technicon Instruments Corporation | Apparatus and method of fluid sample analysis |
US4099917A (en) * | 1977-07-21 | 1978-07-11 | Technicon Instruments Corporation | Process for preparing a cell suspension from blood for discrimination of white blood cells and platelets from other blood particles |
DE2826965A1 (de) * | 1978-06-20 | 1980-01-17 | Boehringer Mannheim Gmbh | Diagnostisches mittel zum nachweis von leukozyten in koerperfluessigkeiten und dafuer geeignete chromogene |
US4581223A (en) * | 1980-03-12 | 1986-04-08 | Lawrence Kass | Individual leukocyte determination by means of differential metachromatic dye sorption |
US4492752A (en) * | 1982-09-03 | 1985-01-08 | Ortho Diagnostics Systems Inc. | Method for discriminating between unstained and absorbing dye stained cells |
CA1254134A (en) * | 1984-03-28 | 1989-05-16 | Mount Sinai School Of Medicine Of The City University Of New York | Specific binding flow cytometry method |
US4654312A (en) * | 1984-05-14 | 1987-03-31 | Becton, Dickinson And Company | Lysing agent for analysis of peripheral blood cells |
US4751179A (en) * | 1984-05-31 | 1988-06-14 | Coulter Electronics, Inc. | Method and reagents for differential determination of four populations of leukocytes in blood |
AU602129B2 (en) * | 1985-09-06 | 1990-10-04 | Technicon Instruments Corportion | Method for the determination of a differential white blood cell count |
US4801549A (en) * | 1985-09-06 | 1989-01-31 | Technicon Instruments Corporation | Method for the determination of a differential white blood cell count |
US4978624A (en) * | 1985-09-06 | 1990-12-18 | Technicon Instruments Corporation | Reagent for the determination of a differential white blood cell count |
US5389549A (en) * | 1987-05-29 | 1995-02-14 | Toa Medical Electronics Co., Ltd. | Method for classifying leukocytes and a reagent used therefor |
AU3182289A (en) * | 1988-02-10 | 1989-09-22 | Nygene Corporation | Process for producing biochemicals |
US5316725A (en) * | 1991-06-07 | 1994-05-31 | Edward Lawrence Carver, Jr. | Reagent system for the improved determination of white blood cell subpopulations |
US5262329A (en) * | 1991-06-13 | 1993-11-16 | Carver Jr Edward L | Method for improved multiple species blood analysis |
US6046019A (en) * | 1991-07-09 | 2000-04-04 | Goumeniouk; Alexander P. | Diagnostic kits and methods for making granulocyte cell counts |
JP3359921B2 (ja) * | 1992-02-24 | 2002-12-24 | クールター インターナショナル コーポレイション | 血液組成物用懸濁媒体及びその使用方法 |
US6509192B1 (en) * | 1992-02-24 | 2003-01-21 | Coulter International Corp. | Quality control method |
DE69329274T2 (de) * | 1992-02-24 | 2001-04-12 | Coulter Corp | Hämatologisches Kontrollprodukt mit Leukozytenanalogen; und Methoden zu ihrer Herstellung und Anwendung |
US6362003B1 (en) | 1992-02-24 | 2002-03-26 | Coulter Corporation | Hematological reference control composition containing leukocyte analogs, methods of making, and uses thereof |
US6812032B1 (en) * | 1993-01-21 | 2004-11-02 | Cdc Technologies, Inc. | Apparatus and method for making a plurality of reagent mixtures and analyzing particle distributions of the reagent mixtures |
US5599501A (en) * | 1994-11-10 | 1997-02-04 | Ciba Corning Diagnostics Corp. | Incubation chamber |
WO1996034283A1 (en) * | 1995-04-27 | 1996-10-31 | Hematronix, Inc. | Lytic system utilizing propionic acid for leukocytes differentiation |
US5639630A (en) * | 1995-05-16 | 1997-06-17 | Bayer Corporation | Method and reagent composition for performing leukocyte differential counts on fresh and aged whole blood samples, based on intrinsic peroxidase activity of leukocytes |
US5843608A (en) * | 1995-06-08 | 1998-12-01 | Coulter International Corp. | Reagent and method for differential determination of leukocytes in blood |
US5686308A (en) * | 1995-06-08 | 1997-11-11 | Coulter Corporation | Reagent and method for differential determination of leukocytes in blood |
US6146901A (en) * | 1997-06-16 | 2000-11-14 | Hematronix, Inc. | Composition for manipulating optical and electrical properties of particles to achieve target values for such properties and methods for using the composition |
US6759246B1 (en) | 2001-11-30 | 2004-07-06 | Research & Diagnostic Systems, Inc. | Hematology control composition including lymphocyte analogs and method for preparation and use |
CA2428740A1 (en) | 2002-05-20 | 2003-11-20 | Bayer Corporation | Automated method and reagent therefor for assaying body fluid samples such as cerebrospinal fluid (csf) |
EP2525222A1 (de) | 2011-05-17 | 2012-11-21 | Markus M. Heiss | Anfängliche relative Lymphozytenzahl als vorhersagbarer Biomarker |
DE212017000208U1 (de) | 2016-09-27 | 2019-04-08 | Novelis, Inc. | System für das berührungslose Spannen eines Metallstreifens |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2807416A (en) * | 1953-07-13 | 1957-09-24 | Ohio Commw Eng Co | Device for automatically counting blood cells |
DE1200577B (de) * | 1962-05-29 | 1965-09-09 | Habil Hans Meyer Doering Dr Me | Anordnung zum Zaehlen der in einem stroemungs-faehigen und lichtdurchlaessigen Medium mitgefuehrten, die Helligkeit eines auf sie gerichteten Lichtstrahles beeinflussenden Teilchen |
US3413464A (en) * | 1965-04-29 | 1968-11-26 | Ibm | Method for measuring the nucleic acid in biological cells after enhancement in an acidic solution |
US3523733A (en) * | 1966-01-05 | 1970-08-11 | Technicon Corp | Method and apparatus for particle counting |
US3427135A (en) * | 1966-07-11 | 1969-02-11 | Technicon Instr | Hematology apparatus |
-
1970
- 1970-10-30 US US00085333A patent/US3741875A/en not_active Expired - Lifetime
-
1971
- 1971-10-21 CA CA125,753A patent/CA960947A/en not_active Expired
- 1971-10-21 AU AU34847/71A patent/AU460469B2/en not_active Expired
- 1971-10-22 BE BE774311A patent/BE774311A/xx not_active IP Right Cessation
- 1971-10-26 GB GB4971771A patent/GB1331568A/en not_active Expired
- 1971-10-27 SE SE7113658A patent/SE372417B/xx unknown
- 1971-10-27 DE DE2153479A patent/DE2153479B2/de active Granted
- 1971-10-28 IT IT30496/71A patent/IT961518B/it active
- 1971-10-28 FR FR7138709A patent/FR2113336A5/fr not_active Expired
- 1971-10-29 SU SU711711809A patent/SU611598A3/ru active
- 1971-10-29 JP JP8567171A patent/JPS576932B1/ja active Pending
- 1971-11-01 NL NLAANVRAGE7114995,A patent/NL172463C/xx not_active IP Right Cessation
-
1980
- 1980-07-03 JP JP9002780A patent/JPS56163455A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
BE774311A (fr) | 1972-04-24 |
JPS576932B1 (de) | 1982-02-08 |
SE372417B (de) | 1974-12-23 |
FR2113336A5 (de) | 1972-06-23 |
DE2153479B2 (de) | 1975-06-05 |
NL172463C (nl) | 1983-09-01 |
AU460469B2 (en) | 1975-04-24 |
US3741875A (en) | 1973-06-26 |
IT961518B (it) | 1973-12-10 |
NL7114995A (de) | 1972-05-03 |
JPS56163455A (en) | 1981-12-16 |
GB1331568A (en) | 1973-09-26 |
AU3484771A (en) | 1973-05-03 |
CA960947A (en) | 1975-01-14 |
DE2153479A1 (de) | 1972-05-04 |
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