GB1331568A - Process for obtaining a differential white blood cell count - Google Patents

Process for obtaining a differential white blood cell count

Info

Publication number
GB1331568A
GB1331568A GB4971771A GB4971771A GB1331568A GB 1331568 A GB1331568 A GB 1331568A GB 4971771 A GB4971771 A GB 4971771A GB 4971771 A GB4971771 A GB 4971771A GB 1331568 A GB1331568 A GB 1331568A
Authority
GB
United Kingdom
Prior art keywords
white blood
cell
cells
detecting
ester
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4971771A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mount Sinai Hospital Research Foundation Inc
Original Assignee
Mount Sinai Hospital Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mount Sinai Hospital Research Foundation Inc filed Critical Mount Sinai Hospital Research Foundation Inc
Publication of GB1331568A publication Critical patent/GB1331568A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/305—Fixative compositions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/808—Optical sensing apparatus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/117497—Automated chemical analysis with a continuously flowing sample or carrier stream
    • Y10T436/118339—Automated chemical analysis with a continuously flowing sample or carrier stream with formation of a segmented stream

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Ecology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1331568 Detecting white blood cells MOUNT SINAI RESEARCH FOUNDATION 26 Oct 1971 [30 Oct 1970] 49717/71 Heading B1X A process for detecting a type of white blood cell comprises adding a cytological fixing agent to a solution containing white blood cells to kill the white blood cells and to immobilise catalytic enzymes within the cells without seriously damaging their catalytic properties and without precipitating soluble components present in the solution, adding a cytochemical substrate to the solution containing the killed cells to enzymatically stain the type of white blood cell, and detecting the stained cell. The stain-forming substance added depends upon what type of cell it is desired to detect; the reaction is a consequence of the different enzymes present in different types of cell. Eosinophils and neutrophils may be stained with 4-chloro-1-naphthol and monocytes with a naphthyl ester, indexyl ester or 8-hydroquinoline ester. The process is applicable to blood, spinal fluid 'and bone marrow.
GB4971771A 1970-10-30 1971-10-26 Process for obtaining a differential white blood cell count Expired GB1331568A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US8533370A 1970-10-30 1970-10-30

Publications (1)

Publication Number Publication Date
GB1331568A true GB1331568A (en) 1973-09-26

Family

ID=22190904

Family Applications (1)

Application Number Title Priority Date Filing Date
GB4971771A Expired GB1331568A (en) 1970-10-30 1971-10-26 Process for obtaining a differential white blood cell count

Country Status (12)

Country Link
US (1) US3741875A (en)
JP (2) JPS576932B1 (en)
AU (1) AU460469B2 (en)
BE (1) BE774311A (en)
CA (1) CA960947A (en)
DE (1) DE2153479B2 (en)
FR (1) FR2113336A5 (en)
GB (1) GB1331568A (en)
IT (1) IT961518B (en)
NL (1) NL172463C (en)
SE (1) SE372417B (en)
SU (1) SU611598A3 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4581223A (en) * 1980-03-12 1986-04-08 Lawrence Kass Individual leukocyte determination by means of differential metachromatic dye sorption

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2125699B2 (en) * 1971-05-25 1973-05-17 Phywe AG, 3400 Gottingen PREPARATION OF CELLS FOR THE MEASUREMENT OF DNA
US4049381A (en) * 1976-03-23 1977-09-20 Technicon Instruments Corporation Apparatus and method of fluid sample analysis
US4099917A (en) * 1977-07-21 1978-07-11 Technicon Instruments Corporation Process for preparing a cell suspension from blood for discrimination of white blood cells and platelets from other blood particles
DE2826965A1 (en) * 1978-06-20 1980-01-17 Boehringer Mannheim Gmbh DIAGNOSTIC AGENT FOR DETECTING LEUCOCYTES IN BODY LIQUIDS AND CHROMOGENES SUITABLE FOR THIS
US4492752A (en) * 1982-09-03 1985-01-08 Ortho Diagnostics Systems Inc. Method for discriminating between unstained and absorbing dye stained cells
CA1254134A (en) * 1984-03-28 1989-05-16 Mount Sinai School Of Medicine Of The City University Of New York Specific binding flow cytometry method
US4654312A (en) * 1984-05-14 1987-03-31 Becton, Dickinson And Company Lysing agent for analysis of peripheral blood cells
US4751179A (en) * 1984-05-31 1988-06-14 Coulter Electronics, Inc. Method and reagents for differential determination of four populations of leukocytes in blood
AU602129B2 (en) * 1985-09-06 1990-10-04 Technicon Instruments Corportion Method for the determination of a differential white blood cell count
US4978624A (en) * 1985-09-06 1990-12-18 Technicon Instruments Corporation Reagent for the determination of a differential white blood cell count
US4801549A (en) * 1985-09-06 1989-01-31 Technicon Instruments Corporation Method for the determination of a differential white blood cell count
US5389549A (en) * 1987-05-29 1995-02-14 Toa Medical Electronics Co., Ltd. Method for classifying leukocytes and a reagent used therefor
AU3182289A (en) * 1988-02-10 1989-09-22 Nygene Corporation Process for producing biochemicals
US5316725A (en) * 1991-06-07 1994-05-31 Edward Lawrence Carver, Jr. Reagent system for the improved determination of white blood cell subpopulations
US5262329A (en) * 1991-06-13 1993-11-16 Carver Jr Edward L Method for improved multiple species blood analysis
US6046019A (en) * 1991-07-09 2000-04-04 Goumeniouk; Alexander P. Diagnostic kits and methods for making granulocyte cell counts
DE69328772T2 (en) * 1992-02-24 2001-02-15 Coulter Corp SUSPENSION MEDIA FOR HEMATOLIC COMPOSITIONS AND METHOD FOR THEIR USE
AU665413B2 (en) * 1992-02-24 1996-01-04 Coulter International Corporation Hematology control composition for leukocyte analogs; and methods for their preparation and use
US6362003B1 (en) 1992-02-24 2002-03-26 Coulter Corporation Hematological reference control composition containing leukocyte analogs, methods of making, and uses thereof
US6509192B1 (en) 1992-02-24 2003-01-21 Coulter International Corp. Quality control method
US6812032B1 (en) * 1993-01-21 2004-11-02 Cdc Technologies, Inc. Apparatus and method for making a plurality of reagent mixtures and analyzing particle distributions of the reagent mixtures
US5599501A (en) * 1994-11-10 1997-02-04 Ciba Corning Diagnostics Corp. Incubation chamber
WO1996034283A1 (en) * 1995-04-27 1996-10-31 Hematronix, Inc. Lytic system utilizing propionic acid for leukocytes differentiation
US5639630A (en) * 1995-05-16 1997-06-17 Bayer Corporation Method and reagent composition for performing leukocyte differential counts on fresh and aged whole blood samples, based on intrinsic peroxidase activity of leukocytes
US5686308A (en) * 1995-06-08 1997-11-11 Coulter Corporation Reagent and method for differential determination of leukocytes in blood
US5843608A (en) * 1995-06-08 1998-12-01 Coulter International Corp. Reagent and method for differential determination of leukocytes in blood
US6146901A (en) * 1997-06-16 2000-11-14 Hematronix, Inc. Composition for manipulating optical and electrical properties of particles to achieve target values for such properties and methods for using the composition
US6759246B1 (en) 2001-11-30 2004-07-06 Research & Diagnostic Systems, Inc. Hematology control composition including lymphocyte analogs and method for preparation and use
CA2428740A1 (en) * 2002-05-20 2003-11-20 Bayer Corporation Automated method and reagent therefor for assaying body fluid samples such as cerebrospinal fluid (csf)
EP2525222A1 (en) 2011-05-17 2012-11-21 Markus M. Heiss Initial relative lymphocyte count as predictive biomarker
BR112018015294B1 (en) 2016-09-27 2022-07-12 Novelis Inc METHOD FOR FILLETING A METALLIC SUBSTRATE IN A LAMINATION UNIT AND SYSTEM FOR FILLETING A METALLIC SUBSTRATE IN A LAMINATION UNIT

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2807416A (en) * 1953-07-13 1957-09-24 Ohio Commw Eng Co Device for automatically counting blood cells
DE1200577B (en) * 1962-05-29 1965-09-09 Habil Hans Meyer Doering Dr Me Arrangement for counting the particles carried in a flow-capable and translucent medium that influence the brightness of a light beam directed at them
US3413464A (en) * 1965-04-29 1968-11-26 Ibm Method for measuring the nucleic acid in biological cells after enhancement in an acidic solution
US3523733A (en) * 1966-01-05 1970-08-11 Technicon Corp Method and apparatus for particle counting
US3427135A (en) * 1966-07-11 1969-02-11 Technicon Instr Hematology apparatus

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4581223A (en) * 1980-03-12 1986-04-08 Lawrence Kass Individual leukocyte determination by means of differential metachromatic dye sorption

Also Published As

Publication number Publication date
JPS56163455A (en) 1981-12-16
BE774311A (en) 1972-04-24
DE2153479B2 (en) 1975-06-05
AU460469B2 (en) 1975-04-24
CA960947A (en) 1975-01-14
US3741875A (en) 1973-06-26
AU3484771A (en) 1973-05-03
NL172463C (en) 1983-09-01
NL7114995A (en) 1972-05-03
DE2153479A1 (en) 1972-05-04
SU611598A3 (en) 1978-06-15
JPS576932B1 (en) 1982-02-08
FR2113336A5 (en) 1972-06-23
IT961518B (en) 1973-12-10
SE372417B (en) 1974-12-23

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PE20 Patent expired after termination of 20 years