SE0202552D0 - Recovery of plasmids in an aqueous two-phase system - Google Patents

Recovery of plasmids in an aqueous two-phase system

Info

Publication number
SE0202552D0
SE0202552D0 SE0202552A SE0202552A SE0202552D0 SE 0202552 D0 SE0202552 D0 SE 0202552D0 SE 0202552 A SE0202552 A SE 0202552A SE 0202552 A SE0202552 A SE 0202552A SE 0202552 D0 SE0202552 D0 SE 0202552D0
Authority
SE
Sweden
Prior art keywords
polymer
plasmid dna
aqueous
plasmids
recovery
Prior art date
Application number
SE0202552A
Other languages
English (en)
Inventor
Cecilia Kepka
Folke Tjerneld
Jenny Rhodin
Original Assignee
Amersham Biosciences Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Ab filed Critical Amersham Biosciences Ab
Priority to SE0202552A priority Critical patent/SE0202552D0/sv
Publication of SE0202552D0 publication Critical patent/SE0202552D0/sv
Priority to PCT/SE2003/001016 priority patent/WO2004020629A1/en
Priority to DE60313870T priority patent/DE60313870D1/de
Priority to AT03736399T priority patent/ATE362529T1/de
Priority to JP2004532487A priority patent/JP4699757B2/ja
Priority to EP03736399A priority patent/EP1532243B1/en
Priority to AU2003237740A priority patent/AU2003237740A1/en
Priority to US10/526,089 priority patent/US7553658B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
SE0202552A 2002-08-27 2002-08-27 Recovery of plasmids in an aqueous two-phase system SE0202552D0 (sv)

Priority Applications (8)

Application Number Priority Date Filing Date Title
SE0202552A SE0202552D0 (sv) 2002-08-27 2002-08-27 Recovery of plasmids in an aqueous two-phase system
PCT/SE2003/001016 WO2004020629A1 (en) 2002-08-27 2003-06-17 Recovery of plasmids in an aqueous two-phase system
DE60313870T DE60313870D1 (de) 2002-08-27 2003-06-17 Gewinnung von plasmiden in einem wässrigen zweiphasensystem
AT03736399T ATE362529T1 (de) 2002-08-27 2003-06-17 Gewinnung von plasmiden in einem wässrigen zweiphasensystem
JP2004532487A JP4699757B2 (ja) 2002-08-27 2003-06-17 水性二相系でのプラスミドの回収
EP03736399A EP1532243B1 (en) 2002-08-27 2003-06-17 Recovery of plasmids in an aqueous two-phase system
AU2003237740A AU2003237740A1 (en) 2002-08-27 2003-06-17 Recovery of plasmids in an aqueous two-phase system
US10/526,089 US7553658B2 (en) 2002-08-27 2003-06-17 Recovery of plasmids in an aqueous two-phase system

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE0202552A SE0202552D0 (sv) 2002-08-27 2002-08-27 Recovery of plasmids in an aqueous two-phase system

Publications (1)

Publication Number Publication Date
SE0202552D0 true SE0202552D0 (sv) 2002-08-27

Family

ID=20288830

Family Applications (1)

Application Number Title Priority Date Filing Date
SE0202552A SE0202552D0 (sv) 2002-08-27 2002-08-27 Recovery of plasmids in an aqueous two-phase system

Country Status (8)

Country Link
US (1) US7553658B2 (sv)
EP (1) EP1532243B1 (sv)
JP (1) JP4699757B2 (sv)
AT (1) ATE362529T1 (sv)
AU (1) AU2003237740A1 (sv)
DE (1) DE60313870D1 (sv)
SE (1) SE0202552D0 (sv)
WO (1) WO2004020629A1 (sv)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2597482A1 (en) * 2005-02-11 2006-08-17 Invitrogen Dynal As Method for isolating nucleic acids comprising the use of ethylene glycol multimers
EP1762616A1 (de) * 2005-09-13 2007-03-14 Qiagen GmbH Verfahren zur selektiven Anreicherung von doppelsträngiger DNA aus Nukleinsäuregemischen
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
WO2008079280A1 (en) 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
JP2010530462A (ja) * 2007-06-19 2010-09-09 ジーイー・ヘルスケア・バイオ−サイエンシズ・アーベー ポリマー２相システムおよびその使用
US8999702B2 (en) 2008-06-11 2015-04-07 Emd Millipore Corporation Stirred tank bioreactor
CN105037535A (zh) 2008-12-16 2015-11-11 Emd密理博公司 搅拌槽反应器及方法
EP2571903B1 (en) 2010-05-17 2019-09-04 EMD Millipore Corporation Stimulus responsive polymers for the purification of biomolecules
PT105438B (pt) * 2010-12-14 2014-08-14 Univ Do Minho Isolamento de dna plasmídico em sistemas de duas fases aquosas utilizando polímeros neutros derivatizados com grupos catiónicos
US9169379B2 (en) 2013-07-15 2015-10-27 King Fahd University Of Petroleum And Minerals Aqueous two-phase systems
ES2954376T3 (es) 2017-03-28 2023-11-21 Phase Scient International Ltd Método para diagnosticar de manera precisa una enfermedad mediante biomarcadores de interés en una biopsia líquida
CN114632349A (zh) 2017-06-01 2022-06-17 相达生物科技美国有限公司 用于多孔材料中双水相分离的相分离行为改性剂
US11609232B2 (en) 2017-09-01 2023-03-21 Phase Diagnostics, Inc. Method and device of using aqueous two-phase systems (ATPS) for enhancing diagnostics for sexually transmitted infections
CN111867721A (zh) 2018-01-19 2020-10-30 相达生物科技国际有限公司 使用固-液相***分离和纯化核酸的方法
US11332796B2 (en) * 2018-01-19 2022-05-17 Phase Scientific International, Ltd. Composition and method for concentration and enrichment of nucleic acids
WO2019144016A1 (en) 2018-01-19 2019-07-25 Yin To Chiu Method of isolating exosomes using encapsulation and aqueous micellar system
CN112375109B (zh) * 2020-11-20 2023-03-28 华东理工大学 利用烷基酚聚氧乙烯聚氧丙烯醚npe-108分离纯化螺旋霉素
WO2023174022A1 (en) * 2022-03-16 2023-09-21 The University Of Hong Kong Synergistic control and dynamic assembly of viscoelastic networks and biomolecular condensates by aqueous liquid-liquid phase separation and liquid-solid phase separation (aqll-ls ps2)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9603304D0 (sv) * 1996-09-11 1996-09-11 Pharmacia & Upjohn Ab Process for purifying a compound
CA2277199A1 (en) 1996-09-11 1998-03-19 Hans Ageland A process for purifying apolipoproteins and a composition for use in the process

Also Published As

Publication number Publication date
DE60313870D1 (de) 2007-06-28
US7553658B2 (en) 2009-06-30
EP1532243A1 (en) 2005-05-25
WO2004020629A1 (en) 2004-03-11
AU2003237740A1 (en) 2004-03-19
JP4699757B2 (ja) 2011-06-15
ATE362529T1 (de) 2007-06-15
EP1532243B1 (en) 2007-05-16
US20060166349A1 (en) 2006-07-27
JP2005537004A (ja) 2005-12-08

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