ATE362529T1 - Gewinnung von plasmiden in einem wässrigen zweiphasensystem - Google Patents

Gewinnung von plasmiden in einem wässrigen zweiphasensystem

Info

Publication number
ATE362529T1
ATE362529T1 AT03736399T AT03736399T ATE362529T1 AT E362529 T1 ATE362529 T1 AT E362529T1 AT 03736399 T AT03736399 T AT 03736399T AT 03736399 T AT03736399 T AT 03736399T AT E362529 T1 ATE362529 T1 AT E362529T1
Authority
AT
Austria
Prior art keywords
aqueous
polymer
plasmid dna
phase system
temperature
Prior art date
Application number
AT03736399T
Other languages
English (en)
Inventor
Cecilia Kepka
Jenny Rhodin
Folke Tjerneld
Original Assignee
Ge Healthcare Bio Sciences Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ge Healthcare Bio Sciences Ab filed Critical Ge Healthcare Bio Sciences Ab
Application granted granted Critical
Publication of ATE362529T1 publication Critical patent/ATE362529T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
AT03736399T 2002-08-27 2003-06-17 Gewinnung von plasmiden in einem wässrigen zweiphasensystem ATE362529T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE0202552A SE0202552D0 (sv) 2002-08-27 2002-08-27 Recovery of plasmids in an aqueous two-phase system

Publications (1)

Publication Number Publication Date
ATE362529T1 true ATE362529T1 (de) 2007-06-15

Family

ID=20288830

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03736399T ATE362529T1 (de) 2002-08-27 2003-06-17 Gewinnung von plasmiden in einem wässrigen zweiphasensystem

Country Status (8)

Country Link
US (1) US7553658B2 (de)
EP (1) EP1532243B1 (de)
JP (1) JP4699757B2 (de)
AT (1) ATE362529T1 (de)
AU (1) AU2003237740A1 (de)
DE (1) DE60313870D1 (de)
SE (1) SE0202552D0 (de)
WO (1) WO2004020629A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT105438B (pt) * 2010-12-14 2014-08-14 Univ Do Minho Isolamento de dna plasmídico em sistemas de duas fases aquosas utilizando polímeros neutros derivatizados com grupos catiónicos

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8569477B2 (en) * 2005-02-11 2013-10-29 Life Technologies As Method for isolating nucleic acids comprising the use of ethylene glycol multimers
EP1762616A1 (de) * 2005-09-13 2007-03-14 Qiagen GmbH Verfahren zur selektiven Anreicherung von doppelsträngiger DNA aus Nukleinsäuregemischen
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US20100267933A1 (en) 2006-12-21 2010-10-21 Moya Wilson Purification of proteins
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
JP2010530462A (ja) * 2007-06-19 2010-09-09 ジーイー・ヘルスケア・バイオ−サイエンシズ・アーベー ポリマー２相システムおよびその使用
WO2009151514A1 (en) 2008-06-11 2009-12-17 Millipore Corporation Stirred tank bioreactor
ES2749232T3 (es) 2008-12-16 2020-03-19 Emd Millipore Corp Reactor de tanque agitado y procedimiento
SG10201804385YA (en) 2010-05-17 2018-06-28 Emd Millipore Corp Stimulus responsive polymers for the purification of biomolecules
US9169379B2 (en) 2013-07-15 2015-10-27 King Fahd University Of Petroleum And Minerals Aqueous two-phase systems
US11366111B2 (en) 2017-03-28 2022-06-21 Phase Scientific International, Ltd. Method for accurate diagnosis of a disease targeting biomarkers in liquid biopsy
CN114632349A (zh) 2017-06-01 2022-06-17 相达生物科技美国有限公司 用于多孔材料中双水相分离的相分离行为改性剂
WO2019046563A1 (en) 2017-09-01 2019-03-07 Phase Diagnostics, Inc. METHOD AND DEVICE FOR USING AQUEOUS BIPHASE SYSTEMS (ATPS) TO IMPROVE THE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS
US11332796B2 (en) * 2018-01-19 2022-05-17 Phase Scientific International, Ltd. Composition and method for concentration and enrichment of nucleic acids
CN111867721A (zh) 2018-01-19 2020-10-30 相达生物科技国际有限公司 使用固-液相***分离和纯化核酸的方法
US11479765B2 (en) 2018-01-19 2022-10-25 Phase Scientific International, Ltd. Method of isolating exosomes using encapsulation and aqueous micellar system
CN112375109B (zh) * 2020-11-20 2023-03-28 华东理工大学 利用烷基酚聚氧乙烯聚氧丙烯醚npe-108分离纯化螺旋霉素
WO2023174022A1 (en) * 2022-03-16 2023-09-21 The University Of Hong Kong Synergistic control and dynamic assembly of viscoelastic networks and biomolecular condensates by aqueous liquid-liquid phase separation and liquid-solid phase separation (aqll-ls ps2)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0942935A1 (de) * 1996-09-11 1999-09-22 Pharmacia & Upjohn Aktiebolag Verfahren für die reinigung von apolipoproteinen und zusammensetzung zur verwendung in diesem verfahren.
SE9603304D0 (sv) * 1996-09-11 1996-09-11 Pharmacia & Upjohn Ab Process for purifying a compound

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT105438B (pt) * 2010-12-14 2014-08-14 Univ Do Minho Isolamento de dna plasmídico em sistemas de duas fases aquosas utilizando polímeros neutros derivatizados com grupos catiónicos

Also Published As

Publication number Publication date
SE0202552D0 (sv) 2002-08-27
US7553658B2 (en) 2009-06-30
AU2003237740A1 (en) 2004-03-19
JP2005537004A (ja) 2005-12-08
EP1532243B1 (de) 2007-05-16
WO2004020629A1 (en) 2004-03-11
EP1532243A1 (de) 2005-05-25
US20060166349A1 (en) 2006-07-27
JP4699757B2 (ja) 2011-06-15
DE60313870D1 (de) 2007-06-28

Similar Documents

Publication Publication Date Title
ATE362529T1 (de) Gewinnung von plasmiden in einem wässrigen zweiphasensystem
Subramanian Sensitive separation procedure for Escherichia coli ribosomal proteins and the resolution of high‐molecular‐weight components
EA201990861A1 (ru) Рнк-направляемые модифицирующие нуклеиновые кислоты ферменты и способы их применения
DE69942459D1 (de) Isolierte nukleinsäuremoleküle die t-zellen induzierbare faktoren kodieren (tif), kodierte proteine und ihre anwendung
AR031912A2 (es) Subtilasa modificada, acido nucleico, vector, celula microbiana y metodo para producirla
DK1341808T3 (da) Syntetiske nucleinsyremolekylesammensætninger og fremgangsmåder til fremstilling
ATE518886T1 (de) Verfahren zur änderung der bindungsspezifität von plasmaproteinen durch oxidations- reduktionsreaktionen
ATE500323T1 (de) Subtilisin-variante
EA200702156A1 (ru) Применение белков в качестве деэмульгаторов
WO2004036189A3 (en) Methods for separating molecules
WO2004057017A3 (en) Detection of small nucleic acids
Chong et al. Identification and Characterization of the Sulfolobus s olfataricus P2 Proteome
ATE378426T1 (de) Neue sequenz um die expression einer nukleinsäure zu erhöhen
WO2002010393A3 (en) Isolated nucleic acid molecules which encode t cell inducible factors, or interleukin-21, the proteins encoded, and uses thereof
WO2004056986A3 (en) Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
ATE411975T1 (de) Verfahren zur aufreinigung von diacerein
BR9810650A (pt) Vetor para expressão de proteìna heteróloga e métodos para extrair proteìna recombinante e para purificar insulina recombinante isolada
ATE369442T1 (de) In vitro evolution von nukleinsäuren und kodierten polypeptiden
WO2000040757A3 (en) Method of identifying nucleic acids
TW200506067A (en) Method of detecting target nucleotide sequence, detection target structure to be used in embodying the method, process for producing the same and assay kit for detecting target nucleotide sequence
Holck et al. DNA-and RNA-binding proteins of chromatin from Escherichia coli
WO2002012543A3 (de) Zweifarbiges fluorimetrisches proteaseassay
WO2005076766A3 (en) Transient production of pharmaceutically important proteins in plants
DE69919568D1 (de) Verfahren und zusammensetzungen zur trennung von biologischen makromolekülen
PT1322753E (pt) Novas redutases do acido 2,5-diceto-l-gluconico e metodos de utilizacao

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties