RU2021123421A - Автоматизированные способы обработки клеток, модули, инструменты и системы - Google Patents
Автоматизированные способы обработки клеток, модули, инструменты и системы Download PDFInfo
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- 210000004027 cell Anatomy 0.000 claims 26
- 238000001890 transfection Methods 0.000 claims 12
- 210000004962 mammalian cell Anatomy 0.000 claims 8
- 239000013604 expression vector Substances 0.000 claims 6
- 239000012530 fluid Substances 0.000 claims 6
- 238000011084 recovery Methods 0.000 claims 4
- 230000009466 transformation Effects 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 239000013598 vector Substances 0.000 claims 2
- 239000013603 viral vector Substances 0.000 claims 2
- 238000001638 lipofection Methods 0.000 claims 1
- 238000000520 microinjection Methods 0.000 claims 1
- 238000010361 transduction Methods 0.000 claims 1
- 230000026683 transduction Effects 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
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Claims (22)
1. Автоматизированный автономный многомодульный инструмент для редактирования клеток для осуществления направляемого нуклеазой редактирования в клетках млекопитающих, включающий:
корпус, выполненный с возможностью размещения всех или некоторых модулей; емкость, выполненную с возможностью приема клеток млекопитающих; одну или более емкостей, выполненных с возможностью приема векторов экспрессии, где векторы экспрессии остов вирусного вектора и редактирующую кассету; модуль для выращивания клеток для трансфекции; модуль для трансфекции, выполненный с возможностью введения векторов в клетки млекопитающих; модуль для направляемого нуклеазой редактирования, выполненный с возможностью для введенных векторов экспрессии редактировать нуклеиновые кислоты в клетках млекопитающих; процессор, выполненный с возможностью обеспечения работы автоматизированного многомодульного инструмента для редактирования клеток, основываясь на вводе данных пользователем и/или выборе пользователем предварительно подготовленного скрипта; и автоматизированную систему подачи жидкости для перемещения жидкостей из емкости с клетками в модуль для выращивания, из модуля для выращивания в модуль для трансформации и из модуля для трансформации в модуль для направляемого нуклеазой редактирования без участия пользователя.
2. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 1, где автоматизированная система подачи жидкости содержит сиппер или дозатор.
3. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 1, где модуль для направляемого нуклеазой редактирования является также модулем восстановления.
4. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 1, дополнительно включающий модуль восстановления, отдельный от модуля редактирования.
5. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 1, где модуль для выращивания отделен от модуля для трансфекции.
6. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 1, где модуль для выращивания и модуль для трансфекции являются одним и тем же модулем.
7. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 1, дополнительно содержащий модуль для отбора.
8. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 7, где модуль для отбора является тем же модулем, что и модуль для направляемого нуклеазой редактирования.
9. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 1, где модуль для трансфекции обеспечивает условия для вирусной трансдукции.
10. Автоматизированный автономный многомодульный инструмент для редактирования клеток для осуществления направляемого нуклеазой редактирования в клетках млекопитающих, включающий:
корпус, выполненный с возможностью размещения всех или некоторых модулей; емкость, выполненную с возможностью приема клеток млекопитающих; одну или более емкостей, выполненных с возможностью приема векторов экспрессии, где векторы экспрессии содержат остов вирусного вектора и редактирующую кассету; модуль для выращивания клеток для трансфекции; модуль для трансфекции, выполненный с возможностью введения векторов в клетки млекопитающих; модуль для направляемого нуклеазой редактирования, выполненный с возможностью для введенных векторов экспрессии редактировать нуклеиновые кислоты в клетках млекопитающих; процессор, выполненный с возможностью обеспечения работы автоматизированного многомодульного инструмента для редактирования клеток, основываясь на вводе данных пользователем и/или выборе пользователем предварительно подготовленного скрипта; и автоматизированную систему подачи жидкости для перемещения жидкостей из емкости с клетками в модуль для выращивания, из модуля для выращивания в модуль для трансформации и из модуля для трансформации в модуль для направляемого нуклеазой редактирования без участия пользователя.
11. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, где автоматизированная система подачи жидкости содержит сиппер или дозатор.
12. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, где модуль для направляемого нуклеазой редактирования является также модулем восстановления.
13. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, дополнительно включающий модуль восстановления, отдельный от модуля редактирования.
14. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, где модуль для выращивания отделен от модуля для трансфекции.
15. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, где модуль для выращивания и модуль для трансфекции являются одним и тем же модулем.
16. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, дополнительно содержащий модуль для отбора.
17. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 16, где модуль для отбора является тем же модулем, что и модуль для направляемого нуклеазой редактирования.
18. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, где модуль для трансфекции обеспечивает условия для липофекции.
19. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, где модуль для трансфекции обеспечивает условия для магнитофекции.
20. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, где модуль для трансфекции обеспечивает условия для микроинъекции.
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US201762567697P | 2017-10-03 | 2017-10-03 | |
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US201862648130P | 2018-03-26 | 2018-03-26 | |
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US201862649731P | 2018-03-29 | 2018-03-29 | |
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US201862657651P | 2018-04-13 | 2018-04-13 | |
US201862657654P | 2018-04-13 | 2018-04-13 | |
US62/657,654 | 2018-04-13 | ||
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US62/671,385 | 2018-05-14 | ||
US62/689,068 | 2018-06-20 | ||
US201862689068P | 2018-06-23 | 2018-06-23 |
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RU2020103731A RU2755308C2 (ru) | 2017-06-30 | 2018-06-30 | Автоматизированный многомодульный инструмент для редактирования клеток (варианты) |
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RU2020103731A RU2755308C2 (ru) | 2017-06-30 | 2018-06-30 | Автоматизированный многомодульный инструмент для редактирования клеток (варианты) |
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2020
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2021
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2022
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