RU2019108582A - КЛЕТКИ SC-β И КОМПОЗИЦИИ И СПОСОБЫ ДЛЯ ИХ СОЗДАНИЯ - Google Patents

КЛЕТКИ SC-β И КОМПОЗИЦИИ И СПОСОБЫ ДЛЯ ИХ СОЗДАНИЯ Download PDF

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RU2019108582A
RU2019108582A RU2019108582A RU2019108582A RU2019108582A RU 2019108582 A RU2019108582 A RU 2019108582A RU 2019108582 A RU2019108582 A RU 2019108582A RU 2019108582 A RU2019108582 A RU 2019108582A RU 2019108582 A RU2019108582 A RU 2019108582A
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inhibitor
signaling pathway
bmp signaling
activator
culture medium
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Куинн П. ПЕТЕРСОН
Фелиция Джей. ПАЙЛИУКА
Дуглас А. МЕЛТОН
Джеффри Р. МИЛЛМАН
Майкл Сарис СЕГЕЛ
Мадс ГЮРТЛЕР
Алиреза РЕЗАНИЯ
Бенджамин ФРАЕР
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Президент Энд Феллоус Оф Гарвард Колледж
Янссен Биотех, Инк.
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Claims (12)

1. Способ дифференцировки PDX1-позитивных панкреатических клеток-предшественников в NKX6.1-позитивные панкреатические клетки-предшественники с помощью культуральной среды, содержащей a) KGF и b) SANT1, причем культуральная среда практически не содержит активатор протеинкиназы С (PKC).
2. Способ по п. 1, отличающийся тем, что активатором PKC является форбол-12,13-дибутират (PdBu) или ТРВ.
3. Способ по п. 1, отличающийся тем, что активатором PKC является PdBu.
4. Способ по п. 1, отличающийся тем, что активатором PCK является ТРВ.
5. Способ по п. 1, дополнительно включающий использование ретиноевой кислоты (RA).
6. Способ дифференцировки PDX1-позитивных панкреатических клеток-предшественников в NKX6.1-позитивные панкреатические клетки-предшественники с помощью культуральной среды, содержащей a) KGF и b) SANT1, причем культуральная среда практически не содержит ингибитор сигнального пути BMP.
7. Способ по п. 6, отличающийся тем, что ингибитором сигнального пути BMP является ноггин, хордин, гремлин и фоллистатин, а также LDN 193189.
8. Способ по п. 6, отличающийся тем, что ингибитором сигнального пути BMP является ноггин.
9. Способ по п. 6, отличающийся тем, что ингибитором сигнального пути BMP является хордин.
10. Способ по п. 6, отличающийся тем, что ингибитором сигнального пути BMP является гремлин.
11. Способ по п. 6, отличающийся тем, что ингибитором сигнального пути BMP является фоллистатин.
12. Способ по п. 6, отличающийся тем, что ингибитором сигнального пути BMP является LDN 193189.
RU2019108582A 2013-06-11 2014-06-11 КЛЕТКИ SC-β И КОМПОЗИЦИИ И СПОСОБЫ ДЛЯ ИХ СОЗДАНИЯ RU2772585C2 (ru)

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US201361833898P 2013-06-11 2013-06-11
US61/833,898 2013-06-11
US201461972212P 2014-03-28 2014-03-28
US61/972,212 2014-03-28

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KR20230135180A (ko) 2023-09-22
MX2019003616A (es) 2019-08-01
US20200385681A1 (en) 2020-12-10
JP2016532436A (ja) 2016-10-20
US20150240212A1 (en) 2015-08-27
US20190169575A1 (en) 2019-06-06
KR20220106230A (ko) 2022-07-28
AU2020203127A1 (en) 2020-06-04
BR112015030946A8 (pt) 2020-01-28
GB2531952B (en) 2017-02-08
EP3008170A1 (en) 2016-04-20
AU2020203127C1 (en) 2023-12-21
US20220162562A1 (en) 2022-05-26
JP6893527B2 (ja) 2021-06-23
KR102631225B1 (ko) 2024-01-31
AU2014284677B2 (en) 2020-03-12
AU2020289721A1 (en) 2021-01-21
GB2557508A9 (en) 2018-09-05
KR20240014630A (ko) 2024-02-01
US10655106B2 (en) 2020-05-19
GB2557508A (en) 2018-06-20
WO2014201167A1 (en) 2014-12-18
CN106414718A (zh) 2017-02-15
AU2020203127B2 (en) 2023-06-22
KR102361924B1 (ko) 2022-02-14
KR20220025151A (ko) 2022-03-03
CN105579577A (zh) 2016-05-11
KR20210156337A (ko) 2021-12-24
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