NO841993L - Immunologiske metoder for paavisning av maligne celler med metastatisk celleaktivitet, samt preparater for utfoerelse av slike metoder - Google Patents

Immunologiske metoder for paavisning av maligne celler med metastatisk celleaktivitet, samt preparater for utfoerelse av slike metoder

Info

Publication number: NO841993L
Authority: NO; Norway
Prior art keywords: type; antibody; antigen; collagenase; enzyme
Prior art date: 1983-05-19

Application number

NO841993A

Other languages

English (en)

Norwegian (no)

Inventor

Karl Tryggvason

Lance Allen Liotta

Original Assignee

Karl Tryggvason

Liotta Lance A

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-05-19

Filing date

1984-05-18

Publication date

1984-11-20

1984-05-18 Application filed by Karl Tryggvason, Liotta Lance A filed Critical Karl Tryggvason

1984-11-20 Publication of NO841993L publication Critical patent/NO841993L/no

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6489—Metalloendopeptidases (3.4.24)
- C12N9/6491—Matrix metalloproteases [MMP's], e.g. interstitial collagenase (3.4.24.7); Stromelysins (3.4.24.17; 3.2.1.22); Matrilysin (3.4.24.23)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
- G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
- G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
- G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
- G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
- G01N2333/96486—Metalloendopeptidases (3.4.24)
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2474/00—Immunochemical assays or immunoassays characterised by detection mode or means of detection
- G01N2474/20—Immunohistochemistry assay
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/811—Test for named disease, body condition or organ function
- Y10S436/813—Cancer

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Biochemistry (AREA)
Immunology (AREA)
General Health & Medical Sciences (AREA)
Molecular Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Wood Science & Technology (AREA)
Biophysics (AREA)
Bioinformatics & Cheminformatics (AREA)
Medicinal Chemistry (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Analytical Chemistry (AREA)
Physics & Mathematics (AREA)
Cell Biology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Enzymes And Modification Thereof (AREA)

NO841993A 1983-05-19 1984-05-18 Immunologiske metoder for paavisning av maligne celler med metastatisk celleaktivitet, samt preparater for utfoerelse av slike metoder NO841993L (no)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US06/496,230 US4677058A (en)	1983-05-19	1983-05-19	Detecting malignant cells with monoclonal antibodies specific to type IV collagenase enzyme

Publications (1)

Publication Number	Publication Date
NO841993L true NO841993L (no)	1984-11-20

Family

ID=23971775

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO841993A NO841993L (no)	1983-05-19	1984-05-18	Immunologiske metoder for paavisning av maligne celler med metastatisk celleaktivitet, samt preparater for utfoerelse av slike metoder

Country Status (26)

Country	Link
US (1)	US4677058A (de)
JP (1)	JPS602187A (de)
AU (1)	AU2808784A (de)
BE (1)	BE899663A (de)
CA (1)	CA1230052A (de)
CH (1)	CH668838A5 (de)
DD (1)	DD251208A5 (de)
DE (1)	DE3418625A1 (de)
DK (1)	DK243184A (de)
ES (1)	ES8605904A1 (de)
FI (1)	FI841897A (de)
FR (1)	FR2546302B1 (de)
GB (1)	GB2142030B (de)
HU (1)	HU195004B (de)
IL (1)	IL71802A0 (de)
IT (1)	IT1175842B (de)
LU (1)	LU85366A1 (de)
NL (1)	NL8401616A (de)
NO (1)	NO841993L (de)
NZ (1)	NZ208108A (de)
PL (1)	PL247745A1 (de)
PT (1)	PT78610B (de)
RO (1)	RO92427A (de)
SE (1)	SE8402703L (de)
YU (1)	YU86984A (de)
ZA (1)	ZA843539B (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6294172B1 (en)	1983-08-12	2001-09-25	Dade Behring Marburg Gmbh	Monoclonal antibodies with specificity for membrane-associated antigens
US4859581A (en) *	1986-03-10	1989-08-22	Board Of Regents, The University Of Texas System	Endoglycosidase assay
US4923818A (en) *	1987-09-04	1990-05-08	Washington University	DNA clone of human type IV collagenase
GB2209526B (en) *	1987-09-04	1992-06-03	Univ Washington	Dna clone of human type iv collagenase
US4876332A (en) *	1987-10-08	1989-10-24	Regents Of The Univeristy Of Minnesota	Polypeptides with type IV collagen activity
US5280106A (en) *	1988-05-20	1994-01-18	Liotta Lance A	Matrix metalloproteinase peptides: role in diagnosis and therapy
US5030555A (en) *	1988-09-12	1991-07-09	University Of Florida	Membrane-strip reagent serodiagnostic apparatus and method
DE3909799A1 (de) *	1989-03-24	1990-09-27	Behringwerke Ag	Monoklonale antikoerper (mak) gegen tumorassoziierte antigene, ihre herstellung und verwendung
US4992537A (en) *	1989-05-15	1991-02-12	Washington University	cDNA encoding 92-kDA type IV collagenase
CA2067797C (en) *	1989-10-18	1997-03-04	Charles R. King	Production and use of human nm23 protein and antibodies therefor
JPH0734014B2 (ja) *	1990-01-26	1995-04-12	アメリカ合衆国	コラーゲナーゼを定量的に測定するための方法
WO1992020820A1 (en) *	1991-05-15	1992-11-26	Vanderbilt University	Method to determine metastatic potential of tumor cells
US5324634A (en) *	1992-03-31	1994-06-28	The Research Foundation Of State University Of New York	Diagnostic tests measuring gelatinase/inhibitor complexes for detection of aggressive and metastatic cancer
JP2673929B2 (ja) *	1993-04-12	1997-11-05	富士薬品工業株式会社	ヒト間質型コラゲナーゼと阻害剤との複合体の免疫学的定量法および臨床診断への応用
EP1789195B1 (de) *	2004-08-26	2010-10-27	Life Technologies Corporation	Elektrobenetzende abgabevorrichtungen und dazugehörige verfahren

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
SE343949B (de) *	1966-06-02	1972-03-20	Pharmacia Ab
DE2363854C3 (de) *	1973-12-21	1980-01-03	Behringwerke Ag, 3550 Marburg	Substrat zur Proteinasebestimmung sowie dessen Herstellung
US4201763A (en) *	1975-10-09	1980-05-06	Bio-Rad Laboratories, Inc.	Solid phase immunofluorescent assay method
FR2515046B1 (fr) *	1981-10-27	1985-11-29	Hybritech Inc	Anticorps monoclonaux marques par un radionucleide et leur application a la visualisation d'une tumeur
US4424278A (en) *	1981-11-16	1984-01-03	Research Corporation	Cancer detection procedure using an acyl carrier protein
US4443367A (en) *	1982-04-08	1984-04-17	Monsanto Company	Peptide and peptolide substrates for mammalian collagenase

1983
- 1983-05-19 US US06/496,230 patent/US4677058A/en not_active Expired - Lifetime
1984
- 1984-05-09 NZ NZ208108A patent/NZ208108A/en unknown
- 1984-05-10 IL IL71802A patent/IL71802A0/xx unknown
- 1984-05-10 ZA ZA843539A patent/ZA843539B/xx unknown
- 1984-05-10 GB GB08411959A patent/GB2142030B/en not_active Expired
- 1984-05-11 FI FI841897A patent/FI841897A/fi not_active Application Discontinuation
- 1984-05-11 CA CA000454194A patent/CA1230052A/en not_active Expired
- 1984-05-15 BE BE0/212938A patent/BE899663A/fr not_active IP Right Cessation
- 1984-05-16 AU AU28087/84A patent/AU2808784A/en not_active Abandoned
- 1984-05-17 DK DK243184A patent/DK243184A/da not_active Application Discontinuation
- 1984-05-17 LU LU85366A patent/LU85366A1/fr unknown
- 1984-05-17 RO RO84114584A patent/RO92427A/ro unknown
- 1984-05-18 NL NL8401616A patent/NL8401616A/nl not_active Application Discontinuation
- 1984-05-18 PT PT78610A patent/PT78610B/pt unknown
- 1984-05-18 DE DE19843418625 patent/DE3418625A1/de not_active Withdrawn
- 1984-05-18 HU HU841936A patent/HU195004B/hu unknown
- 1984-05-18 PL PL24774584A patent/PL247745A1/xx unknown
- 1984-05-18 IT IT20998/84A patent/IT1175842B/it active
- 1984-05-18 ES ES532597A patent/ES8605904A1/es not_active Expired
- 1984-05-18 DD DD84263171A patent/DD251208A5/de unknown
- 1984-05-18 YU YU00869/84A patent/YU86984A/xx unknown
- 1984-05-18 SE SE8402703A patent/SE8402703L/xx not_active Application Discontinuation
- 1984-05-18 NO NO841993A patent/NO841993L/no unknown
- 1984-05-18 FR FR8407747A patent/FR2546302B1/fr not_active Expired
- 1984-05-18 CH CH2471/84A patent/CH668838A5/de not_active IP Right Cessation
- 1984-05-18 JP JP59101622A patent/JPS602187A/ja active Pending

Also Published As

Publication number	Publication date
FI841897A0 (fi)	1984-05-11
FR2546302B1 (fr)	1988-03-04
IL71802A0 (en)	1984-09-30
PT78610B (en)	1986-07-15
YU86984A (en)	1991-01-28
NL8401616A (nl)	1984-12-17
JPS602187A (ja)	1985-01-08
AU2808784A (en)	1984-11-22
GB2142030B (en)	1987-02-04
SE8402703D0 (sv)	1984-05-18
FR2546302A1 (fr)	1984-11-23
CH668838A5 (de)	1989-01-31
ES532597A0 (es)	1986-04-01
RO92427A (ro)	1987-09-30
IT8420998A0 (it)	1984-05-18
PT78610A (en)	1984-06-01
HUT34261A (en)	1985-02-28
DD251208A5 (de)	1987-11-04
NZ208108A (en)	1988-04-29
DK243184A (da)	1984-11-20
BE899663A (fr)	1984-08-31
GB2142030A (en)	1985-01-09
FI841897A (fi)	1984-11-20
IT1175842B (it)	1987-07-15
ES8605904A1 (es)	1986-04-01
DK243184D0 (da)	1984-05-17
HU195004B (en)	1988-03-28
CA1230052A (en)	1987-12-08
PL247745A1 (en)	1985-08-27
ZA843539B (en)	1985-07-31
SE8402703L (sv)	1984-11-20
DE3418625A1 (de)	1984-11-22
LU85366A1 (fr)	1984-11-19
GB8411959D0 (en)	1984-06-13
IT8420998A1 (it)	1985-11-18
US4677058A (en)	1987-06-30

Publication	Publication Date	Title
Zamarron et al.	1990	Monoclonal antibodies specific for a conformationally altered state of fibrinogen
US5912158A (en)	1999-06-15	Prostate specific antigen (PSA)-proteinase inhibitor complexes
Schuppan et al.	1986	Radioimmunoassay for the carboxy-terminal cross-linking domain of type IV (basement membrane) procollagen in body fluids. Characterization and application to collagen type IV metabolism in fibrotic liver disease.
NO841993L (no)	1984-11-20	Immunologiske metoder for paavisning av maligne celler med metastatisk celleaktivitet, samt preparater for utfoerelse av slike metoder
EP0344134A2 (de)	1989-11-29	Monoklonaler Antikörper, spezifisch für eine in transformierten Zellen exprimierte Fibronectin-Sequenz, Hybridoma, das diesen Antikörper erzeugt und Verwendung dieses monoklonalen Antikörpers zur Diagnose von Tumoren
US5767247A (en)	1998-06-16	Anti-annexin-V monoclonal antibodies, and preparation and use thereof
Scully et al.	1988	AIDS-related Kaposi's sarcoma displays differential expression of endothelial surface antigens.
ES2328306T3 (es)	2009-11-11	Anticuerpo monospecifico reactivo con friginogeno y fibrinopeptido b.
US20150219638A1 (en)	2015-08-06	Pivka-ii measurement method, measurement reagent, and measurement kit
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AU2006213411B2 (en)	2010-09-23	Antibody for assay of ADAMTS13 activity and method of activity assay
US4816400A (en)	1989-03-28	Basement membrane collagen degrading enzyme and method of purifying same
KR910008637B1 (ko)	1991-10-19	심근 미오신 중사슬에 대한 단일클론항체
US4808528A (en)	1989-02-28	Antibody composition for the detection of malignant cells with metastatic cell activity
Jemmerson et al.	1982	Convenient selection of monoclonal antibodies to isoenzymes of placental alkaline phosphatase using the catalytic activity of the antigen in enzyme-antigen immunoassay
US20080305499A1 (en)	2008-12-11	Anti-Synoviolin Antibody
Chinni et al.	1998	Cathepsin D antigenic epitopes identified by the humoral responses of ovarian cancer patients
US5635596A (en)	1997-06-03	Peptides derived from the pS2 protein
Chao et al.	1985	Characterization of monoclonal and polyclonal antibodies to human tissue kallikrein.
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Itami et al.	1986	Monoclonal antibodies to the β-andrenergic receptor: Modulation of catecholamine-sensitive adenylate cyclase by the antibody
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