MXPA05008231A - Vector de alta expresion para celulas animales. - Google Patents

Vector de alta expresion para celulas animales.

Info

Publication number
MXPA05008231A
MXPA05008231A MXPA05008231A MXPA05008231A MXPA05008231A MX PA05008231 A MXPA05008231 A MX PA05008231A MX PA05008231 A MXPA05008231 A MX PA05008231A MX PA05008231 A MXPA05008231 A MX PA05008231A MX PA05008231 A MXPA05008231 A MX PA05008231A
Authority
MX
Mexico
Prior art keywords
cells
overexpression vector
animal
animal cell
promoter
Prior art date
Application number
MXPA05008231A
Other languages
English (en)
Inventor
Tetsuo Nakamura
Original Assignee
Japan Immuno Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Immuno Inc filed Critical Japan Immuno Inc
Publication of MXPA05008231A publication Critical patent/MXPA05008231A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un objeto de la presente invencion consiste en proveer un vector de expresion que permita obtener con facilidad cepas con alto nivel de produccion mediante una celula de mamifero, y especialmente una celula de ovario de hamster chino (celula CHO) como huesped. La presente invencion provee un vector de expresion que induce alta productividad de proteinas geneticamente recombinantes en celulas de huesped animal, que comprende un promotor inducible de alta expresion, un sitio de clonacion multiple para la insercion del gen y una secuencia de senal de poliadenilacion, en este orden corriente arriba, y que ademas comprende corriente abajo un gen resistente al farmaco que carece de un promotor capaz de actuar en celulas animales.
MXPA05008231A 2003-02-03 2004-02-03 Vector de alta expresion para celulas animales. MXPA05008231A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003025677 2003-02-03
PCT/JP2004/001066 WO2004070030A1 (ja) 2003-02-03 2004-02-03 動物細胞用高発現ベクター

Publications (1)

Publication Number Publication Date
MXPA05008231A true MXPA05008231A (es) 2006-01-17

Family

ID=32844112

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA05008231A MXPA05008231A (es) 2003-02-03 2004-02-03 Vector de alta expresion para celulas animales.

Country Status (10)

Country Link
EP (1) EP1591523A4 (es)
JP (1) JPWO2004070030A1 (es)
CN (1) CN1771324A (es)
AR (1) AR043017A1 (es)
BR (1) BRPI0406995A (es)
CA (1) CA2514941A1 (es)
MX (1) MXPA05008231A (es)
TN (1) TNSN05187A1 (es)
WO (1) WO2004070030A1 (es)
ZA (1) ZA200506196B (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2716766A1 (en) * 2008-02-27 2009-09-03 Fuso Pharmaceutical Industries, Ltd. Expression vector for mass production of foreign gene-derived protein using animal cell and use thereof
JP5396071B2 (ja) 2008-12-05 2014-01-22 独立行政法人海洋研究開発機構 細胞の増殖を抑制する方法
KR101655492B1 (ko) * 2008-12-22 2016-09-07 국립대학법인 홋가이도 다이가쿠 동물세포를 사용해서 외래유전자 유래 단백질을 대량으로 생산하기 위한 발현 벡터, 및 그의 이용
US9212367B2 (en) 2009-08-06 2015-12-15 Cmc Icos Biologics, Inc. Methods for improving recombinant protein expression
EP2598636A4 (en) * 2010-07-26 2014-03-05 Adv Biolog Inc VECTORS AND METHODS FOR EXPRESSION OF RECOMBINANT PROTEINS
CN102559734B (zh) * 2010-12-24 2014-10-29 神州细胞工程有限公司 一种可用于外源基因表达的载体及细胞株筛选方法
CN104694568B (zh) * 2013-12-10 2018-03-09 深圳先进技术研究院 动物细胞用表达载体、其制备方法及应用
WO2016130583A1 (en) 2015-02-13 2016-08-18 The Board Of Trustees Of The Leland Stanford Junior University Non-silencing selectable marker genes and methods of use
EA201992001A1 (ru) 2017-03-17 2020-01-24 Адверум Байотекнолоджис, Инк. Композиции и способы повышения экспрессии генов

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ210501A (en) * 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
AU582288B2 (en) * 1986-03-07 1989-03-16 Damon Biotech Inc. Vector and method for achieving high level expression in eukaryotic cells
CA1339413C (en) * 1988-06-24 1997-09-02 Junichi Miyazaki Exogenous gene expression vector containing chick .beta.-actin gene promoter
AU7444191A (en) * 1990-02-28 1991-09-18 Schering Corporation Mammalian expression vectors
JPH04218383A (ja) * 1990-05-14 1992-08-07 Green Cross Corp:The プラスミド、形質転換された動物細胞及び異種蛋白質の製造方法
CA2078721A1 (en) * 1991-09-24 1993-03-25 Hiroshi Yonemura Process for preparing human coagulation factor viii protein complex
WO1996023890A1 (fr) * 1995-02-03 1996-08-08 Asahi Glass Company Ltd. Gene de signal de secretion et vecteur d'expression comprenant ce signal
JP3582965B2 (ja) * 1996-08-23 2004-10-27 株式会社イムノ・ジャパン 哺乳動物細胞用発現ベクター

Also Published As

Publication number Publication date
BRPI0406995A (pt) 2006-01-10
JPWO2004070030A1 (ja) 2006-05-25
EP1591523A1 (en) 2005-11-02
CN1771324A (zh) 2006-05-10
WO2004070030A1 (ja) 2004-08-19
AR043017A1 (es) 2005-07-13
CA2514941A1 (en) 2004-08-19
TNSN05187A1 (en) 2007-06-11
EP1591523A4 (en) 2007-07-11
ZA200506196B (en) 2006-10-25

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Legal Events

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