BR0316510A - Vetor de expressão, processo para a preparação de produtos gênicos heterólogos e processo de seleção para células recombinantes de alta produção - Google Patents
Vetor de expressão, processo para a preparação de produtos gênicos heterólogos e processo de seleção para células recombinantes de alta produçãoInfo
- Publication number
- BR0316510A BR0316510A BR0316510-8A BR0316510A BR0316510A BR 0316510 A BR0316510 A BR 0316510A BR 0316510 A BR0316510 A BR 0316510A BR 0316510 A BR0316510 A BR 0316510A
- Authority
- BR
- Brazil
- Prior art keywords
- expression vector
- preparation
- cells
- heterologous gene
- recombinant cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
"VETOR DE EXPRESSãO, PROCESSO PARA A PREPARAçãO DE PRODUTOS GêNICOS HETERóLOGOS E PROCESSO DE SELEçãO PARA CéLULAS RECOMBINANTES DE ALTA PRODUçãO". Um vetor de expressão para células eucarióticas que abrange um gene que codifica uma proteína de interesse, em combinação funcional com um promotor ubiquitina/S27a de hamster e um gene que codifica uma proteína fluorescente. De preferência, o vetor de expressão contém, ainda, um gene marcador da seleção amplificável. São descritas, ainda, células hospedeiras transfectadas com o vetor de expressão, de preferência, células de mamíferos, processos para a preparação de produtos gênicos heterólogos e um processo para a seleção de células de alta produção.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10256083A DE10256083A1 (de) | 2002-11-29 | 2002-11-29 | Expressionsvektor, Verfahren zur Herstellung von heterologen Genprodukten und Selektionsverfahren für hochproduzierende rekombinante Zellen |
PCT/EP2003/013225 WO2004050879A1 (de) | 2002-11-29 | 2003-11-25 | Expressionsvektor, verfahren zur herstellung von heterologen genprodukten und selektionsverfahren für hochproduzierende rekombinante zellen |
Publications (1)
Publication Number | Publication Date |
---|---|
BR0316510A true BR0316510A (pt) | 2005-10-04 |
Family
ID=32403676
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR0316510-8A Expired - Fee Related BR0316510A (pt) | 2002-11-29 | 2003-11-25 | Vetor de expressão, processo para a preparação de produtos gênicos heterólogos e processo de seleção para células recombinantes de alta produção |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP1567648A1 (pt) |
JP (1) | JP2006507829A (pt) |
KR (1) | KR100820035B1 (pt) |
CN (1) | CN1717486A (pt) |
AR (1) | AR042244A1 (pt) |
AU (1) | AU2003292102A1 (pt) |
BR (1) | BR0316510A (pt) |
CA (1) | CA2507714A1 (pt) |
DE (1) | DE10256083A1 (pt) |
MX (1) | MXPA05005482A (pt) |
TW (1) | TWI321152B (pt) |
WO (1) | WO2004050879A1 (pt) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080124760A1 (en) * | 2006-07-26 | 2008-05-29 | Barbara Enenkel | Regulatory Nucleic Acid Elements |
AU2008339985C1 (en) | 2007-12-21 | 2012-12-20 | Novartis Ag | Mammalian expression vector |
MX2011009025A (es) | 2009-02-27 | 2011-09-28 | Novartis Ag | Sistema de vector de expresion que comprende dos marcadores de seleccion. |
KR101239495B1 (ko) * | 2011-01-21 | 2013-03-05 | 경상대학교산학협력단 | αΑ-크리스탈린 유전자를 발현하는 재조합 아데노바이러스 및 이를 이용한 망막혈관 질환의 유전자 치료 |
EP2716756B1 (en) | 2011-05-25 | 2021-12-08 | National University Corporation Okayama University | Reic-expressing adenovirus vector for use in treating cancer |
FR2981946B1 (fr) * | 2011-10-28 | 2015-02-20 | Lfb Biotechnologies | Unites de transcription et leur utilisation dans des vecteurs d'expression (yb2/0) |
ES2689136T3 (es) | 2013-07-31 | 2018-11-08 | Novartis Ag | Nuevos vectores de selección y métodos de selección de células hospedadoras eucariotas |
CN104404072B (zh) * | 2014-11-24 | 2018-04-27 | 浙江大学 | 用标记基因预测外源基因表达量及筛选转基因家蚕的方法 |
CN106442685B (zh) * | 2016-10-25 | 2019-03-26 | 山东大学 | 一种快速低成本筛选蛋白质表达条件的方法 |
KR20210030949A (ko) * | 2018-07-13 | 2021-03-18 | 론자 리미티드 | 내인성 단백질의 수준을 감소시켜 생물학적 생성물의 생산을 개선하는 방법 |
WO2020227206A1 (en) * | 2019-05-07 | 2020-11-12 | Amgen Inc. | Vectors and expression systems for producing recombinant proteins |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5179017A (en) * | 1980-02-25 | 1993-01-12 | The Trustees Of Columbia University In The City Of New York | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
US4656134A (en) * | 1982-01-11 | 1987-04-07 | Board Of Trustees Of Leland Stanford Jr. University | Gene amplification in eukaryotic cells |
EP0173552B1 (en) | 1984-08-24 | 1991-10-09 | The Upjohn Company | Recombinant dna compounds and the expression of polypeptides such as tpa |
US5491084A (en) * | 1993-09-10 | 1996-02-13 | The Trustees Of Columbia University In The City Of New York | Uses of green-fluorescent protein |
WO1995007463A1 (en) * | 1993-09-10 | 1995-03-16 | The Trustees Of Columbia University In The City Of New York | Uses of green fluorescent protein |
DE19539493A1 (de) * | 1995-10-24 | 1997-04-30 | Thomae Gmbh Dr K | Starker homologer Promotor aus Hamster |
WO1998032869A1 (en) * | 1997-01-29 | 1998-07-30 | Neurosearch A/S | Expression vectors and methods for in vivo expression of therapeutic polypeptides |
DK1196566T3 (da) * | 1999-07-12 | 2006-06-06 | Genentech Inc | Ekspressionsvektorer og -metoder |
EP1258255A1 (en) * | 2001-05-18 | 2002-11-20 | Boehringer Ingelheim International GmbH | Conjugates of an antibody to CD44 and a maytansinoid |
-
2002
- 2002-11-29 DE DE10256083A patent/DE10256083A1/de not_active Ceased
-
2003
- 2003-11-25 JP JP2004556182A patent/JP2006507829A/ja active Pending
- 2003-11-25 WO PCT/EP2003/013225 patent/WO2004050879A1/de active Application Filing
- 2003-11-25 AU AU2003292102A patent/AU2003292102A1/en not_active Abandoned
- 2003-11-25 MX MXPA05005482A patent/MXPA05005482A/es not_active Application Discontinuation
- 2003-11-25 CN CNA2003801044261A patent/CN1717486A/zh active Pending
- 2003-11-25 KR KR1020057009693A patent/KR100820035B1/ko not_active IP Right Cessation
- 2003-11-25 BR BR0316510-8A patent/BR0316510A/pt not_active Expired - Fee Related
- 2003-11-25 CA CA002507714A patent/CA2507714A1/en not_active Abandoned
- 2003-11-25 EP EP03767642A patent/EP1567648A1/de not_active Withdrawn
- 2003-11-26 TW TW092133164A patent/TWI321152B/zh not_active IP Right Cessation
- 2003-11-28 AR ARP030104395A patent/AR042244A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
CN1717486A (zh) | 2006-01-04 |
DE10256083A1 (de) | 2004-08-12 |
AU2003292102A1 (en) | 2004-06-23 |
MXPA05005482A (es) | 2005-07-25 |
EP1567648A1 (de) | 2005-08-31 |
WO2004050879A1 (de) | 2004-06-17 |
TWI321152B (en) | 2010-03-01 |
KR100820035B1 (ko) | 2008-04-10 |
TW200502388A (en) | 2005-01-16 |
CA2507714A1 (en) | 2004-06-17 |
JP2006507829A (ja) | 2006-03-09 |
KR20050085203A (ko) | 2005-08-29 |
AR042244A1 (es) | 2005-06-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B08F | Application fees: application dismissed [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 5A,6A E 7A ANUIDADES. |
|
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2073 DE 28/09/2010. |