MX349121B - Produccion recombinante de esteviol glucosidos. - Google Patents

Produccion recombinante de esteviol glucosidos.

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Publication number
MX349121B
MX349121B MX2012014016A MX2012014016A MX349121B MX 349121 B MX349121 B MX 349121B MX 2012014016 A MX2012014016 A MX 2012014016A MX 2012014016 A MX2012014016 A MX 2012014016A MX 349121 B MX349121 B MX 349121B
Authority
MX
Mexico
Prior art keywords
steviol glycosides
steviol
recombinant production
plants
plant cells
Prior art date
Application number
MX2012014016A
Other languages
English (en)
Other versions
MX2012014016A (es
Inventor
M Hicks Paula
M Kishore Ganesh
Motion Michael
Hansen Jorgen
Houghton-Larsen Jens
Halkjaer Hansen Esben
Dalgaard Mikkelsen Michael
Tavares Sabina
Blom Charlotte
Original Assignee
Evolva Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Inc filed Critical Evolva Inc
Publication of MX2012014016A publication Critical patent/MX2012014016A/es
Publication of MX349121B publication Critical patent/MX349121B/es

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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Seasonings (AREA)

Abstract

La presente invención se refiere a un método para transferir una porción de azúcar adicional a una posición C2´de una glucosa en un esteviol glicósido, dicho método está caracterizado porque comprende poner en contacto dicho esteviol glicósido con un polipéptido recombinante capaz de la 1, 2 glicosilación del C2´ de la 13-O-glucosa, 19-O-glucosa, o ambos 13-O-glucosa y 19-O-glucosa de un esteviol glicósido y un UDP-azúcar bajo condiciones de reacción adecuadas para la transferencia de dicha porción de azúcar adicional a dicho esteviol glicósido, en donde el esteviol glicósido es esteviol-13-O-glucósido, rubusósido, esteviósido, o rebaudiósido A, y en donde un esteviósido, rebaudiósido E, rebaudiósido D, esteviol-1,2 biósido, esteviol-1,2-xilobiósido, esteviol-1,2-ramnobiósido, un isómero del mismo, y/o una composición de esteviol glicósido del mismo se produce después de la transferencia de la porción de azúcar adicional.
MX2012014016A 2010-06-02 2011-06-02 Produccion recombinante de esteviol glucosidos. MX349121B (es)

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