MX344268B - Proceso para purificacion de proteinas. - Google Patents

Proceso para purificacion de proteinas.

Info

Publication number
MX344268B
MX344268B MX2013004091A MX2013004091A MX344268B MX 344268 B MX344268 B MX 344268B MX 2013004091 A MX2013004091 A MX 2013004091A MX 2013004091 A MX2013004091 A MX 2013004091A MX 344268 B MX344268 B MX 344268B
Authority
MX
Mexico
Prior art keywords
proteins
purification
processes
sample
protein
Prior art date
Application number
MX2013004091A
Other languages
English (en)
Other versions
MX2013004091A (es
Inventor
K Hickman Robert
Wang Chen
Original Assignee
Abbvie Inc *
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbvie Inc * filed Critical Abbvie Inc *
Publication of MX2013004091A publication Critical patent/MX2013004091A/es
Publication of MX344268B publication Critical patent/MX344268B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/12—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the preparation of the feed
    • B01D15/125—Pre-filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/30—Partition chromatography
    • B01D15/305—Hydrophilic interaction chromatography [HILIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847—Multimodal interactions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Water Supply & Treatment (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

La presente invención se refiere a un método para purificar una proteína. El método involucra proporcionar una muestra conteniendo la proteína, procesar la muestra a través de una resma de cromatografía de captura, inactivar virus en la muestra y procesar a través de al menos un filtro de profundidad y membrana de intercambio iónico.
MX2013004091A 2010-10-11 2011-10-11 Proceso para purificacion de proteinas. MX344268B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39176210P 2010-10-11 2010-10-11
PCT/US2011/055691 WO2012051147A1 (en) 2010-10-11 2011-10-11 Processes for purification of proteins

Publications (2)

Publication Number Publication Date
MX2013004091A MX2013004091A (es) 2013-06-07
MX344268B true MX344268B (es) 2016-12-09

Family

ID=45938674

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013004091A MX344268B (es) 2010-10-11 2011-10-11 Proceso para purificacion de proteinas.

Country Status (15)

Country Link
US (1) US20120264920A1 (es)
EP (1) EP2627425A4 (es)
JP (1) JP6023715B2 (es)
KR (1) KR20130142128A (es)
CN (1) CN103379949B (es)
AU (2) AU2011316730B2 (es)
BR (1) BR112013008738B1 (es)
CA (1) CA2813747A1 (es)
IL (1) IL225650A0 (es)
MX (1) MX344268B (es)
NZ (1) NZ608943A (es)
RU (1) RU2610667C2 (es)
SG (2) SG10201508401TA (es)
TW (1) TW201221641A (es)
WO (1) WO2012051147A1 (es)

Families Citing this family (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201702922VA (en) * 2008-10-20 2017-06-29 Abbvie Inc Isolation and purification of antibodies using protein a affinity chromatography
US9029517B2 (en) 2010-07-30 2015-05-12 Emd Millipore Corporation Chromatography media and method
US9062106B2 (en) 2011-04-27 2015-06-23 Abbvie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
EP2768532A4 (en) 2011-10-18 2015-03-11 Coherus Biosciences Inc FORMANTS OF STANOLPT STABILIZED WITH SODIUM CHLORIDE
HRP20230065T1 (hr) 2011-10-28 2023-03-03 Prothena Biosciences Limited Humanizirana protutijela koja prepoznaju alfa-sinuklein
EP2773439A4 (en) * 2011-10-31 2015-07-01 Merck Sharp & Dohme CHROMATOGRAPHY METHOD FOR DECOMPOSING HETEROGENEOUS ANTIBODY AGGREGATES
SG11201404321YA (en) 2012-01-27 2014-08-28 Neotope Biosciences Ltd Humanized antibodies that recognize alpha-synuclein
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9150645B2 (en) 2012-04-20 2015-10-06 Abbvie, Inc. Cell culture methods to reduce acidic species
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
WO2013177118A2 (en) * 2012-05-21 2013-11-28 Abbvie Inc. Novel purification of non-human antibodies using protein a affinity chromatography
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
US9809799B2 (en) * 2012-06-29 2017-11-07 Emd Millipore Corporation Methods for inactivating viruses during a protein purification process
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
KR20150043523A (ko) 2012-09-02 2015-04-22 애브비 인코포레이티드 단백질 불균일성의 제어 방법
CN110051823A (zh) * 2012-09-11 2019-07-26 科荣生生物科学公司 高纯度和优异产量的正确折叠的依那西普
UA118441C2 (uk) 2012-10-08 2019-01-25 Протена Біосаєнсиз Лімітед Антитіло, що розпізнає альфа-синуклеїн
CA2905010A1 (en) 2013-03-12 2014-09-18 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
CN104236983A (zh) * 2013-06-14 2014-12-24 中国科学院大连化学物理研究所 溶液样品中含长烷基链的离子液体的去除方法
AR096713A1 (es) * 2013-06-25 2016-01-27 Cadila Healthcare Ltd Proceso de purificación para anticuerpos monoclonales
US10513555B2 (en) * 2013-07-04 2019-12-24 Prothena Biosciences Limited Antibody formulations and methods
CN105339388B (zh) * 2013-07-12 2021-06-15 Emd密理博公司 确定利用活性炭从包含靶蛋白的样品去除病毒的方法
AU2014310255B2 (en) * 2013-08-21 2017-02-16 Cadila Healthcare Limited Purification process for PTH
EP3052640A2 (en) 2013-10-04 2016-08-10 AbbVie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
WO2015057910A1 (en) 2013-10-16 2015-04-23 Oncobiologics, Inc. Buffer formulations for enhanced antibody stability
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
CN105980047B (zh) 2013-12-12 2019-01-04 Emd密理博公司 使用含丙烯酰胺的过滤器分离蛋白
MY181081A (en) * 2013-12-27 2020-12-17 Chugai Pharmaceutical Co Ltd Method for purifying antibody having low isoelectric point
WO2015133972A1 (en) * 2014-03-07 2015-09-11 Agency For Science, Technology And Research Apparatus and methods for fractionation of biological products
EP3116891B1 (en) * 2014-03-10 2020-02-12 Richter Gedeon Nyrt. Immunoglobulin purification using pre-cleaning steps
CN105017418B (zh) * 2014-03-27 2021-02-23 上海药明康德新药开发有限公司 单克隆抗体纯化工艺方法
WO2016036508A1 (en) 2014-09-02 2016-03-10 Emd Millipore Corporation High surface area fiber media with nano-fibrillated surface features
KR102162753B1 (ko) * 2014-12-08 2020-10-07 이엠디 밀리포어 코포레이션 혼합층 이온 교환 흡착제
EP4212546A1 (en) * 2014-12-10 2023-07-19 OPKO Biologics Ltd. Methods of producing long acting ctp-modified polypeptides
US20160176921A1 (en) * 2014-12-22 2016-06-23 Alexion Pharmaceuticals, Inc. Methods of purifying recombinant proteins
WO2016118707A1 (en) 2015-01-21 2016-07-28 Oncobiologics, Inc. Modulation of charge variants in a monoclonal antibody composition
EP3015542A1 (de) 2015-05-07 2016-05-04 Bayer Technology Services GmbH Modulare anlage und verfahren zur kontinuierlichen, keimreduzierten produktion und/oder aufbereitung eines produktes
WO2016207328A1 (en) * 2015-06-24 2016-12-29 Glycotope Gmbh PROCESS FOR THE PURIFICATION OF γ-CARBOXYLATED POLYPEPTIDES
WO2017027861A1 (en) 2015-08-13 2017-02-16 Amgen Inc. Charged depth filtration of antigen-binding proteins
MX2018009341A (es) 2016-02-03 2019-05-15 Oncobiologics Inc Formulaciones tamponadas para una mayor estabilidad de anticuerpos.
US10416137B2 (en) * 2016-09-07 2019-09-17 Board Of Regents, University Of Texas System Electrodialytic capillary suppressor for suppressed conductometric ion chromatography
CN106749660B (zh) * 2016-12-27 2020-02-14 嘉和生物药业有限公司 单克隆抗体下游纯化过程中有效去除宿主蛋白的方法
WO2018140887A1 (en) * 2017-01-30 2018-08-02 Regeneron Pharmaceuticals, Inc. Method for reducing bioburden in chromatography
AU2018326458A1 (en) * 2017-08-30 2020-02-20 Ares Trading S.A. Method for purifying proteins
IL311002A (en) * 2017-12-21 2024-04-01 Genzyme Corp Methods for improved removal of impurities during protein A chromatography
CN108059650A (zh) * 2018-01-05 2018-05-22 上海药明生物技术有限公司 低pH病毒灭活工艺中的纯化方法
WO2019183334A1 (en) 2018-03-21 2019-09-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
MX2020010018A (es) 2018-03-26 2020-10-14 Boehringer Ingelheim Animal Health Usa Inc Metodo para producir una composicion inmunogenica.
EP3546475A1 (en) * 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
US10792618B2 (en) * 2018-06-19 2020-10-06 Sartorius Stedim Biotech Gmbh Particle separation and/or purification of a fluid
JP7397874B2 (ja) 2018-08-30 2023-12-13 エイチシーダブリュー バイオロジックス インコーポレイテッド 多鎖キメラポリペプチドおよびその使用
JP7407822B2 (ja) 2018-08-30 2024-01-04 エイチシーダブリュー バイオロジックス インコーポレイテッド 単鎖キメラポリペプチドおよびその使用
CN109369776B (zh) * 2018-11-09 2020-12-25 杭州奕安济世生物药业有限公司 一种蛋白纯化***及方法
MX2022001166A (es) * 2019-08-01 2022-02-23 Regeneron Pharma Metodo para inactivacion viral.
AU2021208632A1 (en) * 2020-01-17 2022-08-04 Regeneron Pharmaceuticals, Inc. Hydrophobic interaction chromatography for viral clearance
US20210277054A1 (en) * 2020-02-11 2021-09-09 HCW Biologics, Inc. Chromatography resin and uses thereof
CN111440226B (zh) * 2020-05-27 2022-06-03 杭州奕安济世生物药业有限公司 蛋白纯化的***和方法
US12024545B2 (en) 2020-06-01 2024-07-02 HCW Biologics, Inc. Methods of treating aging-related disorders
WO2022072899A1 (en) * 2020-10-02 2022-04-07 Regeneron Pharmaceuticals, Inc. Continuous virus retentive filtration
CN114181300A (zh) * 2021-12-20 2022-03-15 方坦思(上海)生物医药有限公司 一种高纯度单克隆抗体的制备方法
CN114409765A (zh) * 2021-12-23 2022-04-29 苏州创胜医药集团有限公司 抗体的纯化方法
CN114133446A (zh) * 2021-12-31 2022-03-04 方坦思(上海)生物医药有限公司 单克隆抗体的纯化方法
CN117736324B (zh) * 2022-09-22 2024-06-28 北京东方百泰生物科技股份有限公司 一种抗Siglec-15单克隆抗体的纯化方法
WO2024059235A2 (en) * 2022-09-16 2024-03-21 Amgen Inc. A method for harvesting products from perfusion cultures
US20240230645A9 (en) * 2022-10-21 2024-07-11 Waters Technologies Corporation Protein capture from raw cell culture using protein a affixed in open tubular and annular helically coiled tubes
WO2024096505A1 (ko) * 2022-10-31 2024-05-10 삼성바이오에피스 주식회사 친화성 크로마토그래피를 통해 불순물을 제거하는 방법

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5118796A (en) * 1987-12-09 1992-06-02 Centocor, Incorporated Efficient large-scale purification of immunoglobulins and derivatives
US5506127A (en) * 1994-09-21 1996-04-09 Proba; Zbigniew Therapeutic grade thrombin produced by chromatography
KR20010034554A (ko) * 1998-03-03 2001-04-25 레이몬드, 엠. 위티 치료제로서의 ｃｄ147 결합 분자
ES2198913T5 (es) * 1998-05-06 2013-11-18 Genentech, Inc. Purificación de proteínas mediante cromatografía de intercambio iónico
SE9802213D0 (sv) * 1998-06-18 1998-06-18 Amersham Pharm Biotech Ab A method for the removal/purification of serum albumins and means for use in the method
US6214221B1 (en) * 1999-02-22 2001-04-10 Henry B. Kopf Method and apparatus for purification of biological substances
US6652853B2 (en) * 2001-03-08 2003-11-25 Ludwig Institute For Cancer Research Method for treating cancer using A33 specific antibodies and chemotherapeutic agents
EP1401870A4 (en) * 2001-05-24 2006-04-19 Human Genome Sciences ANTIBODIES AGAINST TUMOR NECROSIS FACTOR DELTA (APRIL)
AU2003262258A1 (en) * 2002-08-30 2004-03-29 Arkray, Inc. Method of purifying protein and glucose dehydrogenase
US7777006B2 (en) * 2002-12-31 2010-08-17 Csl Behring L.L.C. Method for purification of alpha-1-antitrypsin
KR20060033870A (ko) * 2003-06-25 2006-04-20 파멕사 에이/에스 Ｈｅｒ-2 변이체의 정제
WO2005082937A2 (en) * 2004-02-27 2005-09-09 Octapharma Ag A method of providing a purified, virus safe antibody preparation
TWI391399B (zh) * 2005-05-25 2013-04-01 Hoffmann La Roche 測定溶離多肽之鹽濃度之方法
CA2632519A1 (en) * 2005-12-06 2007-07-05 Amgen Inc. Polishing steps used in multi-step protein purification processes
BRPI0709726A2 (pt) * 2006-04-05 2011-07-26 Abbott Biotechnology Ltd. purificaÇço de anticorpo
EP2069387A4 (en) * 2006-06-14 2011-02-02 Glaxosmithkline Llc METHODS OF PURIFYING ANTIBODIES USING HYDROXYAPATITE CERAMIC
NZ574626A (en) * 2006-08-28 2011-12-22 Ares Trading Sa Process for removing free Fc-moieties from fluids comprising Fc-containing proteins using ion exchange chromatography
WO2008073620A2 (en) * 2006-11-02 2008-06-19 Neose Technologies, Inc. Manufacturing process for the production of polypeptides expressed in insect cell-lines
US7691980B2 (en) * 2007-01-09 2010-04-06 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
US20080214795A1 (en) * 2007-02-14 2008-09-04 Amgen Inc. Method of isolating antibodies by precipitation
US8188242B2 (en) * 2008-04-08 2012-05-29 Bio-Rad Laboratories, Inc. Chromatography purification of antibodies
WO2010043703A1 (en) * 2008-10-17 2010-04-22 Dsm Ip Assets B.V. Removal of host cell proteins
NZ603619A (en) * 2008-10-20 2014-05-30 Abbvie Inc Antibodies that bind to il-12 and methods of purifying the same
SG10201702922VA (en) * 2008-10-20 2017-06-29 Abbvie Inc Isolation and purification of antibodies using protein a affinity chromatography
AU2009307737B2 (en) * 2008-10-20 2015-07-23 Abbvie Inc. Viral inactivation during purification of antibodies
KR20110071011A (ko) * 2008-10-20 2011-06-27 아보트 러보러터리즈 Ｉｌ-18에 결합하는 항체 및 이의 정제 방법
CN102395597A (zh) * 2009-03-11 2012-03-28 惠氏有限责任公司 纯化小模块免疫药物蛋白的方法
GB0910591D0 (en) * 2009-06-19 2009-07-29 Immunobiology Ltd Method for the purification of protein complexes
AU2010275764A1 (en) * 2009-07-21 2012-03-15 Transgene Sa Enzymatic composition for the digestion of chicken embryos

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IL225650A0 (en) 2013-06-27
MX2013004091A (es) 2013-06-07
BR112013008738A2 (pt) 2015-10-06
KR20130142128A (ko) 2013-12-27
RU2013120948A (ru) 2014-11-20
NZ608943A (en) 2015-04-24
AU2011316730B2 (en) 2015-12-10
AU2016201535A1 (en) 2016-03-31
JP2013539787A (ja) 2013-10-28
SG10201508401TA (en) 2015-11-27
EP2627425A1 (en) 2013-08-21
CA2813747A1 (en) 2012-04-19
EP2627425A4 (en) 2014-11-05
CN103379949A (zh) 2013-10-30
WO2012051147A1 (en) 2012-04-19
US20120264920A1 (en) 2012-10-18
BR112013008738B1 (pt) 2017-12-19
RU2610667C2 (ru) 2017-02-14
JP6023715B2 (ja) 2016-11-09
SG189872A1 (en) 2013-06-28
AU2011316730A1 (en) 2013-05-02
TW201221641A (en) 2012-06-01

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