SG10201807572PA - Robust antibody purification - Google Patents

Robust antibody purification

Info

Publication number
SG10201807572PA
SG10201807572PA SG10201807572PA SG10201807572PA SG10201807572PA SG 10201807572P A SG10201807572P A SG 10201807572PA SG 10201807572P A SG10201807572P A SG 10201807572PA SG 10201807572P A SG10201807572P A SG 10201807572PA SG 10201807572P A SG10201807572P A SG 10201807572PA
Authority
SG
Singapore
Prior art keywords
antibody purification
robust antibody
robust
hcps
separation
Prior art date
Application number
SG10201807572PA
Inventor
Romas Skudas
Matthias Joehnck
Bianca Edelmann
Simon Braun
Mikhail Kozlov
Matthew Stone
Kevin Galipeau
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Publication of SG10201807572PA publication Critical patent/SG10201807572PA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)

Abstract

ROBUST ANTIBODY PURIFICATION The present invention refers to a method for the separation of host cell proteins (HCPs), antibody fragments and low molecular weight substances from solutions containing antibodies. (No suitable figure)
SG10201807572PA 2014-03-04 2015-02-18 Robust antibody purification SG10201807572PA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201461947761P 2014-03-04 2014-03-04

Publications (1)

Publication Number Publication Date
SG10201807572PA true SG10201807572PA (en) 2018-10-30

Family

ID=52589333

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201607341SA SG11201607341SA (en) 2014-03-04 2015-02-18 Robust antibody purification
SG10201807572PA SG10201807572PA (en) 2014-03-04 2015-02-18 Robust antibody purification

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201607341SA SG11201607341SA (en) 2014-03-04 2015-02-18 Robust antibody purification

Country Status (10)

Country Link
US (1) US10457720B2 (en)
EP (1) EP3114455B1 (en)
JP (2) JP7037275B2 (en)
KR (1) KR102405382B1 (en)
CN (1) CN106103477B (en)
CA (1) CA2942134C (en)
DK (1) DK3114455T3 (en)
ES (1) ES2784638T3 (en)
SG (2) SG11201607341SA (en)
WO (1) WO2015131978A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6845139B2 (en) * 2014-12-15 2021-03-17 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングＭｅｒｃｋ Ｐａｔｅｎｔ Ｇｅｓｅｌｌｓｃｈａｆｔ ｍｉｔ ｂｅｓｃｈｒａｅｎｋｔｅｒ Ｈａｆｔｕｎｇ Capture target molecules from crude solution
US11186858B1 (en) 2016-03-15 2021-11-30 Fresenius Kabi Deutschland Gmbh Methods for increasing biosimilarity
JP7068316B2 (en) * 2016-09-15 2022-05-16 クラヴェゴ ゲーエムベーハー アンド コー カーゲー Utilization of polymer mesh for purification of macromolecules
CN106674403A (en) * 2017-01-03 2017-05-17 宏葵生物（中国）有限公司 Preparation method of polystyrene microspheres for enhancing immunoturbidimetry and application thereof
CN111770931B (en) * 2018-02-20 2023-08-25 高丽大学校产学协力团 Multi-column for separating exosomes and exosome separation method
JP7404689B2 (en) * 2018-08-06 2023-12-26 東ソー株式会社 Antibody adsorbent with immobilized Fc-binding protein and antibody separation method using the same
WO2022260091A1 (en) * 2021-06-10 2022-12-15 三菱ケミカル株式会社 Synthetic adsorbent, antibody purification method, and antibody production method
AU2022317368A1 (en) * 2021-07-29 2024-02-22 Csl Behring Ag Method of purifying immunoglobulin g and uses thereof

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4382124B1 (en) 1958-07-18 1994-10-04 Rohm & Haas Process for preparing macroreticular resins, copolymers and products of said process
JPS5798504A (en) 1980-12-11 1982-06-18 Mitsubishi Monsanto Chem Co Production of vinyl polymer
JP2534979B2 (en) 1984-12-27 1996-09-18 東ソー株式会社 Filler for liquid chromatography
JPS61141704A (en) 1985-11-11 1986-06-28 Mitsubishi Kasei Vinyl Co Production of vinyl polymer
US6251996B1 (en) * 1998-12-09 2001-06-26 The Dow Chemical Company Gel-type copolymer bead and ion-exchange resins made therefrom
DE60102327T3 (en) 2000-08-11 2012-07-05 Rohm And Haas Co. Polymer Absorbents and Related Manufacturing Process
US20060234226A1 (en) * 2002-04-26 2006-10-19 Fahner Robert L Non-affinity purification of proteins
DE602004026343D1 (en) * 2003-10-24 2010-05-12 Amgen Inc PROCESS FOR PURIFYING PROTEINS IN A FLOW FRACTION FROM CHROMATOGRAPHY WITH HYDROPHOBIC INTERACTIONS
CN1927899A (en) * 2006-09-01 2007-03-14 烟台硕德新材料有限公司 Preparation method of micron monodisperse copolymerization microsphere
US20130131318A1 (en) 2010-02-12 2013-05-23 Diderik Reinder Kremer Single unit antibody purification
JP6292718B2 (en) 2011-07-01 2018-03-14 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Method for separating monomeric polypeptides from aggregated polypeptides
US9096648B2 (en) * 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
PL2970378T3 (en) * 2013-03-15 2021-12-06 Biogen Ma Inc. Hydrophobic interaction protein chromatography under no-salt conditions
US8946395B1 (en) * 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
JP6845139B2 (en) * 2014-12-15 2021-03-17 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングＭｅｒｃｋ Ｐａｔｅｎｔ Ｇｅｓｅｌｌｓｃｈａｆｔ ｍｉｔ ｂｅｓｃｈｒａｅｎｋｔｅｒ Ｈａｆｔｕｎｇ Capture target molecules from crude solution

Also Published As

Publication number Publication date
CA2942134A1 (en) 2015-09-11
SG11201607341SA (en) 2016-10-28
US20170073394A1 (en) 2017-03-16
WO2015131978A1 (en) 2015-09-11
EP3114455A1 (en) 2017-01-11
CN106103477B (en) 2021-08-17
KR102405382B1 (en) 2022-06-03
US10457720B2 (en) 2019-10-29
KR20160128411A (en) 2016-11-07
ES2784638T3 (en) 2020-09-29
JP2020128373A (en) 2020-08-27
JP7037275B2 (en) 2022-03-16
JP2017509621A (en) 2017-04-06
EP3114455B1 (en) 2020-01-15
CN106103477A (en) 2016-11-09
JP7153041B2 (en) 2022-10-13
CA2942134C (en) 2023-08-15
DK3114455T3 (en) 2020-04-06

Similar Documents

Publication Publication Date Title
SG10201807572PA (en) Robust antibody purification
CY1124921T1 (en) METHODS FOR THE EXTRACTION AND PURIFICATION OF NON-DENATHERED PROTEINS
PH12017501115A1 (en) Anti-myostatin antibodies, polypeptides containing variant fc regions, and method of use
WO2014165082A3 (en) Antibodies and methods of detection
WO2014200910A3 (en) Bioinformatic processes for determination of peptide binding
MX2016016886A (en) Anti-axl antibodies.
NZ727020A (en) Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
EA201491837A1 (en) CHEMICAL ANTIGENOUS RECEPTORS AIMED AT ANTIGEN OF B-CELL MATURATION
WO2015195453A3 (en) Methods for increasing the capacity of flow-through processes
MX2016010542A (en) Ultrafiltration unit for continuous buffer or media exchange from a protein solution.
PH12016500275B1 (en) Antibodies
MX2017005661A (en) Mammalian cells expressing cytomegalovirus antigens.
PH12021550521A1 (en) Antibodies that potently neutralize rabies virus and other lyssaviruses and uses thereof
MX2017008096A (en) Pestivirus.
MX2017013333A (en) Method for protein purification.
MX2017007381A (en) Binding members for human c-maf.
EP3162895A4 (en) Improved fc-binding protein, method for producing said protein, antibody adsorbent using said protein, and method for separating antibody using said adsorbent
TW201613981A (en) Compositions and methods for the diagnosis of rheumatoid arthritis
EA201792366A1 (en) METHOD FOR SEPARATION OF PROTEIN AND OTHER IMPURITIES AND CAPSULAR POLYSACCHARIDES OF MICROORGANISMS
EP3397066A4 (en) Process for separating proteins from biomass materials
MX2016013584A (en) Porcine parvovirus.
EP3092002A4 (en) Method of purifying monoclonal antibodies
EP3362464A4 (en) Methods for extracting proteins from blood plasma
WO2016075546A3 (en) Antibodies that neutralize ebola virus and uses thereof
WO2013138259A3 (en) Polypeptides for treating and/or limiting influenza infection