MX2023008931A - Complejos de transposoma ligados a superficie de complejo. - Google Patents
Complejos de transposoma ligados a superficie de complejo.Info
- Publication number
- MX2023008931A MX2023008931A MX2023008931A MX2023008931A MX2023008931A MX 2023008931 A MX2023008931 A MX 2023008931A MX 2023008931 A MX2023008931 A MX 2023008931A MX 2023008931 A MX2023008931 A MX 2023008931A MX 2023008931 A MX2023008931 A MX 2023008931A
- Authority
- MX
- Mexico
- Prior art keywords
- transposome complexes
- complex surface
- compositions
- bound transposome
- methods
- Prior art date
Links
- 238000000034 method Methods 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 150000007523 nucleic acids Chemical group 0.000 abstract 2
- 238000012408 PCR amplification Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/507—Recombinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
La presente descripción se relaciona con métodos, composiciones, y kits para generar una genoteca de fragmentos de ácido nucleico marcados sin usar la amplificación por PCR, que incluye métodos y composiciones para la fragmentación y marcación de ácidos nucleicos (p. ej., ADN) usando complejos de transposoma inmovilizados en soporte sólido.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962791509P | 2019-01-11 | 2019-01-11 | |
US201962840610P | 2019-04-30 | 2019-04-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2023008931A true MX2023008931A (es) | 2023-08-10 |
Family
ID=69172787
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2021003625A MX2021003625A (es) | 2019-01-11 | 2020-01-10 | Complejos de transposoma ligados a superficie de complejo. |
MX2023008931A MX2023008931A (es) | 2019-01-11 | 2021-03-26 | Complejos de transposoma ligados a superficie de complejo. |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2021003625A MX2021003625A (es) | 2019-01-11 | 2020-01-10 | Complejos de transposoma ligados a superficie de complejo. |
Country Status (12)
Country | Link |
---|---|
US (3) | US11685946B2 (es) |
EP (1) | EP3908672A1 (es) |
JP (1) | JP2022516821A (es) |
KR (1) | KR20210114918A (es) |
CN (1) | CN112930405A (es) |
AU (1) | AU2020206443A1 (es) |
BR (1) | BR112021006038A2 (es) |
CA (1) | CA3114732A1 (es) |
IL (1) | IL281664B2 (es) |
MX (2) | MX2021003625A (es) |
SG (1) | SG11202102918WA (es) |
WO (1) | WO2020144373A1 (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20230041725A (ko) * | 2020-08-06 | 2023-03-24 | 일루미나, 인코포레이티드 | 비드-연결된 트랜스포좀을 사용한 rna 및 dna 시퀀싱 라이브러리의 제작 |
CN112795563A (zh) * | 2021-03-23 | 2021-05-14 | 上海欣百诺生物科技有限公司 | 生物素化的转座体在回收CUT&Tag或ATAC-seq产物中的用途及方法 |
MX2023010814A (es) * | 2021-03-29 | 2023-09-27 | Illumina Inc | Metodos mejorados de preparacion de genotecas. |
MX2023011218A (es) | 2021-03-31 | 2023-10-02 | Illumina Inc | Métodos de preparación de genotecas de secuenciación de tagmentación direccional usando tecnología basada en transposón con identificadores moleculares únicos para la corrección de errores. |
WO2022216133A1 (ko) * | 2021-04-09 | 2022-10-13 | 주식회사 셀레믹스 | 간소화된 차세대 염기서열 분석 라이브러리 전처리 방법 |
WO2023225095A1 (en) * | 2022-05-18 | 2023-11-23 | Illumina Cambridge Limited | Preparation of size-controlled nucleic acid fragments |
WO2024024583A1 (ja) * | 2022-07-25 | 2024-02-01 | 東洋紡株式会社 | 次世代シーケンサー解析等のための断片化及びタグメント化方法 |
CN116515977B (zh) * | 2023-06-28 | 2024-04-16 | 浙江大学 | 基于单端接头转座酶的单细胞基因组测序试剂盒和方法 |
Family Cites Families (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0450060A1 (en) | 1989-10-26 | 1991-10-09 | Sri International | Dna sequencing |
WO2002004680A2 (en) | 2000-07-07 | 2002-01-17 | Visigen Biotechnologies, Inc. | Real-time sequence determination |
AU2002227156A1 (en) | 2000-12-01 | 2002-06-11 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
EP1530578B1 (en) | 2002-08-23 | 2013-03-13 | Illumina Cambridge Limited | Modified nucleotides for polynucleotide sequencing |
JP2007525571A (ja) | 2004-01-07 | 2007-09-06 | ソレクサ リミテッド | 修飾分子アレイ |
JP2008513782A (ja) | 2004-09-17 | 2008-05-01 | パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド | 分子解析のための装置及び方法 |
GB0427236D0 (en) | 2004-12-13 | 2005-01-12 | Solexa Ltd | Improved method of nucleotide detection |
US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
CN101460953B (zh) | 2006-03-31 | 2012-05-30 | 索雷克萨公司 | 用于合成分析的序列的***和装置 |
AU2007309504B2 (en) | 2006-10-23 | 2012-09-13 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
WO2012106546A2 (en) * | 2011-02-02 | 2012-08-09 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
US9644199B2 (en) | 2012-10-01 | 2017-05-09 | Agilent Technologies, Inc. | Immobilized transposase complexes for DNA fragmentation and tagging |
US9683230B2 (en) * | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
EP3626866B1 (en) | 2013-08-19 | 2021-03-24 | Abbott Molecular Inc. | Next-generation sequencing libraries |
TWI682997B (zh) * | 2014-04-15 | 2020-01-21 | 美商伊路米納有限公司 | 用於改善***序列傾向及增加dna輸入耐受度之經修飾的轉位酶 |
WO2016037361A1 (zh) * | 2014-09-12 | 2016-03-17 | 深圳华大基因科技有限公司 | 试剂盒及其在核酸测序中的用途 |
WO2016061517A2 (en) | 2014-10-17 | 2016-04-21 | Illumina Cambridge Limited | Contiguity preserving transposition |
WO2016189331A1 (en) | 2015-05-28 | 2016-12-01 | Illumina Cambridge Limited | Surface-based tagmentation |
US20170283864A1 (en) * | 2016-03-31 | 2017-10-05 | Agilent Technologies, Inc. | Use of transposase and y adapters to fragment and tag dna |
WO2018013710A1 (en) * | 2016-07-12 | 2018-01-18 | F. Hoffman-La Roche Ag | Primer extension target enrichment |
IL263118B2 (en) * | 2017-02-21 | 2023-12-01 | Illumina Inc | Tegumentation using fixed transpososomes with linkers |
SG11201910070PA (en) | 2017-05-08 | 2019-11-28 | Illumina Inc | Universal short adapters for indexing of polynucleotide samples |
US20180340169A1 (en) * | 2017-05-26 | 2018-11-29 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
EP3704247B1 (en) * | 2017-11-02 | 2023-01-04 | Bio-Rad Laboratories, Inc. | Transposase-based genomic analysis |
US11739367B2 (en) * | 2017-11-08 | 2023-08-29 | Twinstrand Biosciences, Inc. | Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters |
-
2020
- 2020-01-10 IL IL281664A patent/IL281664B2/en unknown
- 2020-01-10 SG SG11202102918WA patent/SG11202102918WA/en unknown
- 2020-01-10 US US16/739,739 patent/US11685946B2/en active Active
- 2020-01-10 EP EP20700876.4A patent/EP3908672A1/en active Pending
- 2020-01-10 CA CA3114732A patent/CA3114732A1/en active Pending
- 2020-01-10 KR KR1020217009366A patent/KR20210114918A/ko unknown
- 2020-01-10 AU AU2020206443A patent/AU2020206443A1/en active Pending
- 2020-01-10 BR BR112021006038-3A patent/BR112021006038A2/pt unknown
- 2020-01-10 CN CN202080005545.5A patent/CN112930405A/zh active Pending
- 2020-01-10 JP JP2021517266A patent/JP2022516821A/ja active Pending
- 2020-01-10 MX MX2021003625A patent/MX2021003625A/es unknown
- 2020-01-10 WO PCT/EP2020/050612 patent/WO2020144373A1/en unknown
-
2021
- 2021-03-26 MX MX2023008931A patent/MX2023008931A/es unknown
- 2021-05-03 US US17/306,592 patent/US11306348B2/en active Active
-
2023
- 2023-05-12 US US18/316,575 patent/US20230340568A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
IL281664A (en) | 2021-05-31 |
SG11202102918WA (en) | 2021-04-29 |
CN112930405A (zh) | 2021-06-08 |
IL281664B1 (en) | 2024-03-01 |
CA3114732A1 (en) | 2020-07-16 |
EP3908672A1 (en) | 2021-11-17 |
US11306348B2 (en) | 2022-04-19 |
BR112021006038A2 (pt) | 2021-10-26 |
US11685946B2 (en) | 2023-06-27 |
IL281664B2 (en) | 2024-07-01 |
AU2020206443A1 (en) | 2021-04-15 |
JP2022516821A (ja) | 2022-03-03 |
US20200224246A1 (en) | 2020-07-16 |
US20230340568A1 (en) | 2023-10-26 |
MX2021003625A (es) | 2021-05-27 |
WO2020144373A1 (en) | 2020-07-16 |
US20210269856A1 (en) | 2021-09-02 |
KR20210114918A (ko) | 2021-09-24 |
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