MX2023004461A - Plantillas de secuenciacion que comprenden multiples insertos y composiciones y metodos para mejorar la productividad de secuenciacion. - Google Patents

Plantillas de secuenciacion que comprenden multiples insertos y composiciones y metodos para mejorar la productividad de secuenciacion.

Info

Publication number
MX2023004461A
MX2023004461A MX2023004461A MX2023004461A MX2023004461A MX 2023004461 A MX2023004461 A MX 2023004461A MX 2023004461 A MX2023004461 A MX 2023004461A MX 2023004461 A MX2023004461 A MX 2023004461A MX 2023004461 A MX2023004461 A MX 2023004461A
Authority
MX
Mexico
Prior art keywords
sequencing
methods
templates
compositions
multiple inserts
Prior art date
Application number
MX2023004461A
Other languages
English (en)
Inventor
Jonathan Mark Boutell
Yir- Shyuan WU
Tarun Khurana
Niall Anthony Gormley
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2023004461A publication Critical patent/MX2023004461A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/101—Crosslinking agents, e.g. psoralen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

En la presente descripción se describe un polinucleótido para usarse como plantilla de secuenciación que comprende múltiples insertos. También se describe en la presente descripción un método para generar y usar estos polinucleótidos y métodos de uso de tales plantillas, que incluyen análisis de información de contigüidad. Además, las plantillas de secuenciación que comprenden una secuencia de inserto y una copia de una secuencia de inserto pueden usarse para corregir errores aleatorios generados durante la secuenciación o amplificación o para identificar el daño a nucleobases u otra mutación que conduce a apareamiento de bases no canónico en un ácido nucleico bicatenario. También se describen en la presente descripción métodos para realizar el análisis de metilación.
MX2023004461A 2020-10-21 2021-10-20 Plantillas de secuenciacion que comprenden multiples insertos y composiciones y metodos para mejorar la productividad de secuenciacion. MX2023004461A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063094422P 2020-10-21 2020-10-21
US202163256040P 2021-10-15 2021-10-15
PCT/US2021/055878 WO2022087150A2 (en) 2020-10-21 2021-10-20 Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput

Publications (1)

Publication Number Publication Date
MX2023004461A true MX2023004461A (es) 2023-05-03

Family

ID=78622058

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023004461A MX2023004461A (es) 2020-10-21 2021-10-20 Plantillas de secuenciacion que comprenden multiples insertos y composiciones y metodos para mejorar la productividad de secuenciacion.

Country Status (10)

Country Link
US (1) US20230407388A1 (es)
EP (1) EP4232600A2 (es)
JP (1) JP2023547366A (es)
KR (1) KR20230091116A (es)
CN (1) CN116438319A (es)
AU (1) AU2021366658A1 (es)
CA (1) CA3198842A1 (es)
IL (1) IL302207A (es)
MX (1) MX2023004461A (es)
WO (1) WO2022087150A2 (es)

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WO2023168300A1 (en) * 2022-03-01 2023-09-07 Guardant Health, Inc. Methods for analyzing cytosine methylation and hydroxymethylation
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WO2024061799A1 (en) 2022-09-19 2024-03-28 Illumina, Inc. Deformable polymers comprising immobilised primers

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Publication number Publication date
JP2023547366A (ja) 2023-11-10
WO2022087150A2 (en) 2022-04-28
US20230407388A1 (en) 2023-12-21
AU2021366658A1 (en) 2023-06-22
CA3198842A1 (en) 2022-04-28
KR20230091116A (ko) 2023-06-22
AU2021366658A9 (en) 2024-05-02
EP4232600A2 (en) 2023-08-30
WO2022087150A3 (en) 2022-06-30
IL302207A (en) 2023-06-01
CN116438319A (zh) 2023-07-14

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