DK3207134T3 - Kontiguitetsbevarende transposition - Google Patents

Kontiguitetsbevarende transposition Download PDF

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Publication number
DK3207134T3
DK3207134T3 DK15797490.8T DK15797490T DK3207134T3 DK 3207134 T3 DK3207134 T3 DK 3207134T3 DK 15797490 T DK15797490 T DK 15797490T DK 3207134 T3 DK3207134 T3 DK 3207134T3
Authority
DK
Denmark
Prior art keywords
contiguity
conversing
transposition
conversing transposition
contiguity conversing
Prior art date
Application number
DK15797490.8T
Other languages
English (en)
Inventor
Frank J Steemers
Fan Zhang
Jason Richard Betley
Niall Anthony Gormley
Wouter Meuleman
Jacqueline Weir
Avgousta Ioannou
Gareth Jenkins
Rosamond Jackson
Natalie Morrell
Dmitry K Pokholok
Steven J Norberg
Amirali Kia
Igor Goryshin
Rigo Pantoja
Kevin L Gunderson
Molly He
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Priority claimed from PCT/US2015/056040 external-priority patent/WO2016061517A2/en
Application granted granted Critical
Publication of DK3207134T3 publication Critical patent/DK3207134T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK15797490.8T 2014-10-17 2015-10-16 Kontiguitetsbevarende transposition DK3207134T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462065544P 2014-10-17 2014-10-17
US201562157396P 2015-05-05 2015-05-05
PCT/US2015/056040 WO2016061517A2 (en) 2014-10-17 2015-10-16 Contiguity preserving transposition

Publications (1)

Publication Number Publication Date
DK3207134T3 true DK3207134T3 (da) 2019-09-23

Family

ID=54601997

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15797490.8T DK3207134T3 (da) 2014-10-17 2015-10-16 Kontiguitetsbevarende transposition

Country Status (6)

Country Link
EP (2) EP3636757A1 (da)
CN (2) CN107969137B (da)
DK (1) DK3207134T3 (da)
ES (1) ES2745814T3 (da)
LT (1) LT3207134T (da)
PT (1) PT3207134T (da)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变***及构建方法
US11773441B2 (en) * 2018-05-03 2023-10-03 Becton, Dickinson And Company High throughput multiomics sample analysis
WO2020061903A1 (zh) * 2018-09-27 2020-04-02 深圳华大生命科学研究院 测序文库的构建方法和得到的测序文库及测序方法
EP3856903A4 (en) 2018-09-27 2022-07-27 Grail, LLC METHYLATION MARKER AND TARGETED METHYLATION PROBE PANEL
CN112867801A (zh) * 2018-11-30 2021-05-28 Illumina公司 使用单一测定分析多种分析物
EP3997223A1 (en) * 2019-07-12 2022-05-18 Illumina Cambridge Limited Compositions and methods for preparing nucleic acid sequencing libraries using crispr/cas9 immobilized on a solid support
KR20220107934A (ko) * 2019-12-02 2022-08-02 일루미나 케임브리지 리미티드 게놈 dna의 증폭된 인접성 보존 라이브러리 단편들의 시간-기반 클러스터 이미징
IL294909A (en) 2020-02-13 2022-09-01 Zymergen Inc A metagenomic library and natural product discovery platform
WO2021232184A1 (zh) * 2020-05-18 2021-11-25 深圳华大智造科技有限公司 标签化的转座复合体及其在高通量测序中的应用
MX2023004461A (es) * 2020-10-21 2023-05-03 Illumina Inc Plantillas de secuenciacion que comprenden multiples insertos y composiciones y metodos para mejorar la productividad de secuenciacion.
CN112795563A (zh) * 2021-03-23 2021-05-14 上海欣百诺生物科技有限公司 生物素化的转座体在回收CUT&Tag或ATAC-seq产物中的用途及方法
WO2023225519A1 (en) 2022-05-17 2023-11-23 10X Genomics, Inc. Modified transposons, compositions and uses thereof
CN115747301B (zh) * 2022-08-01 2023-12-22 深圳赛陆医疗科技有限公司 一种测序文库的构建方法、构建测序文库的试剂盒及基因测序方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
EP1994180A4 (en) * 2006-02-24 2009-11-25 Callida Genomics Inc GENOME SEQUENCING ON DNA ARRAYS WITH HIGH THROUGHPUT
EP3272879B1 (en) * 2008-10-24 2019-08-07 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
KR20130101031A (ko) * 2010-08-27 2013-09-12 제넨테크, 인크. 핵산 포획 및 서열분석을 위한 방법
ES2731460T3 (es) * 2010-10-08 2019-11-15 Harvard College Alto rendimiento de células individuales con código de barra
CA2821299C (en) * 2010-11-05 2019-02-12 Frank J. Steemers Linking sequence reads using paired code tags
US8829171B2 (en) * 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
CA2821559C (en) * 2011-01-28 2017-01-31 Illumina, Inc. Oligonucleotide replacement for di-tagged and directional libraries
EP2670894B1 (en) * 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
WO2012108864A1 (en) 2011-02-08 2012-08-16 Illumina, Inc. Selective enrichment of nucleic acids
NO2694769T3 (da) * 2012-03-06 2018-03-03
AU2013232131B2 (en) 2012-03-13 2018-09-20 Integrated Dna Technologies, Inc. Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
US9644199B2 (en) * 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
JP6222639B2 (ja) * 2013-03-07 2017-11-01 積水メディカル株式会社 メチル化dnaの検出方法
ES2887177T3 (es) * 2013-03-13 2021-12-22 Illumina Inc Método de preparación de una biblioteca de secuenciación de ácidos nucleicos
GB2546833B (en) * 2013-08-28 2018-04-18 Cellular Res Inc Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels
SG11201605344YA (en) * 2013-12-30 2016-07-28 Atreca Inc Analysis of nucleic acids associated with single cells using nucleic acid barcodes

Also Published As

Publication number Publication date
EP3207134A2 (en) 2017-08-23
EP3207134B1 (en) 2019-07-03
CN114045282A (zh) 2022-02-15
CN107969137A (zh) 2018-04-27
LT3207134T (lt) 2019-09-10
ES2745814T3 (es) 2020-03-03
PT3207134T (pt) 2019-09-17
EP3636757A1 (en) 2020-04-15
CN107969137B (zh) 2021-10-26

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